CY1117558T1 - Μεθοδος για καλλιεργεια κυτταρων για παραγωγη ουσιων - Google Patents

Μεθοδος για καλλιεργεια κυτταρων για παραγωγη ουσιων

Info

Publication number
CY1117558T1
CY1117558T1 CY20161100413T CY161100413T CY1117558T1 CY 1117558 T1 CY1117558 T1 CY 1117558T1 CY 20161100413 T CY20161100413 T CY 20161100413T CY 161100413 T CY161100413 T CY 161100413T CY 1117558 T1 CY1117558 T1 CY 1117558T1
Authority
CY
Cyprus
Prior art keywords
glucose
limitation
dgl
qglc
substances
Prior art date
Application number
CY20161100413T
Other languages
English (en)
Inventor
Ruth Essers
Jochen Gaetgens
Thomas Link
Thomas Noll
Kerstin Skopnik
Christian Wandrey
Original Assignee
F. Hoffmann-La Roche Ag
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by F. Hoffmann-La Roche Ag filed Critical F. Hoffmann-La Roche Ag
Publication of CY1117558T1 publication Critical patent/CY1117558T1/el

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0018—Culture media for cell or tissue culture
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/705—Receptors; Cell surface antigens; Cell surface determinants
    • C07K14/70596—Molecules with a "CD"-designation not provided for elsewhere
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/30—Non-immunoglobulin-derived peptide or protein having an immunoglobulin constant or Fc region, or a fragment thereof, attached thereto
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/30—Organic components
    • C12N2500/34—Sugars

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Cell Biology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Medicinal Chemistry (AREA)
  • Biophysics (AREA)
  • Toxicology (AREA)
  • Immunology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

Η εφεύρεση αφορά μέθοδο για καλλιέργεια κυττάρων με σκοπό την παραγωγή ουσιών. Σύμφωνα με την εφεύρεση η καλλιέργεια μίας κυτταρικής σειράς που παράγει ουσίες προχωρά με παράλληλη παροχή θρεπτικού μέσου κατά τρόπο ώστε στο διάλυμα καλλιέργειας να αποκαθίσταται περιορισμός γλυκόζης. Ο βαθμός του περιορισμού γλυκόζης DGL = qGLc/qGlcmax (qGIc = στιγμιαίος παρατηρούμενος, ειδικός ρυθμός κατανάλωσης γλυκόζης· qGlcmax = μέγιστος γνωστός, για τα κύτταρα αυτά, ειδικός ρυθμός κατανάλωσης γλυκόζης). Ο DGL βρίσκεται εντός των ορίων 0 και 1, όπου 0 σημαίνει απόλυτο περιορισμό και 1 σημαίνει απουσία περιορισμού ή απόλυτη περίσσεια γλυκόζης. Σύμφωνα με την εφεύρεση ο DGL είναι μεγαλύτερος ή ίσος με τον DLG που οδηγεί σε αποκλειστική συντήρηση του κυττάρου και ≤ 0,5.
CY20161100413T 2002-11-27 2016-05-13 Μεθοδος για καλλιεργεια κυτταρων για παραγωγη ουσιων CY1117558T1 (el)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE10255508A DE10255508A1 (de) 2002-11-27 2002-11-27 Verfahren zur Kultivierung von Zellen zur Produktion von Substanzen
EP10152393.4A EP2226381B1 (de) 2002-11-27 2003-11-07 Verfahren zur kultivierung von zellen zur produktion von substanzen

Publications (1)

Publication Number Publication Date
CY1117558T1 true CY1117558T1 (el) 2017-04-26

Family

ID=32318751

Family Applications (2)

Application Number Title Priority Date Filing Date
CY20101100518T CY1110267T1 (el) 2002-11-27 2010-06-11 Μεθοδος για την καλλιεργεια κυτταρων για την παραγωγη ουσιων
CY20161100413T CY1117558T1 (el) 2002-11-27 2016-05-13 Μεθοδος για καλλιεργεια κυτταρων για παραγωγη ουσιων

Family Applications Before (1)

Application Number Title Priority Date Filing Date
CY20101100518T CY1110267T1 (el) 2002-11-27 2010-06-11 Μεθοδος για την καλλιεργεια κυτταρων για την παραγωγη ουσιων

Country Status (12)

Country Link
US (2) US20060127975A1 (el)
EP (3) EP2275530B1 (el)
JP (2) JP4469283B2 (el)
AT (1) ATE462013T1 (el)
CY (2) CY1110267T1 (el)
DE (2) DE10255508A1 (el)
DK (2) DK2226381T3 (el)
ES (3) ES2567161T3 (el)
HU (1) HUE028862T2 (el)
PT (1) PT1585810E (el)
SI (3) SI1585810T1 (el)
WO (1) WO2004048556A1 (el)

Families Citing this family (15)

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ES2382488T3 (es) 1997-03-21 2012-06-08 Chugai Seiyaku Kabushiki Kaisha Un agente preventivo o terapéutico para enfermedades mediadas por células t sensibilizadas que comprende un antagonista de il-6 como ingrediente activo
UA80091C2 (en) 2001-04-02 2007-08-27 Chugai Pharmaceutical Co Ltd Remedies for infant chronic arthritis-relating diseases and still's disease which contain an interleukin-6 (il-6) antagonist
GB2401040A (en) * 2003-04-28 2004-11-03 Chugai Pharmaceutical Co Ltd Method for treating interleukin-6 related diseases
EP1623019B2 (en) * 2003-05-15 2017-01-25 Wyeth LLC Restricted glucose feed for animal cell culture
PE20091174A1 (es) 2007-12-27 2009-08-03 Chugai Pharmaceutical Co Ltd Formulacion liquida con contenido de alta concentracion de anticuerpo
DE102008002210A1 (de) * 2008-06-04 2009-12-10 Evonik Degussa Gmbh Verfahren zur fermentativen Herstellung von Erythropoietin
AU2016210748B2 (en) * 2009-10-26 2018-03-01 Chugai Seiyaku Kabushiki Kaisha Method for the production of a glycosylated immunoglobulin
AU2015203309B2 (en) * 2009-10-26 2016-05-05 Chugai Seiyaku Kabushiki Kaisha Method for the production of a glycosylated immunoglobulin
PL2493922T3 (pl) * 2009-10-26 2017-07-31 F.Hoffmann-La Roche Ag Sposób wytwarzania glikozylowanych immunoglobulin
TWI537386B (zh) * 2009-12-22 2016-06-11 Ｄｓｍ智慧財產有限公司 由發酵法獲得的６－胺己酸製備己內醯胺
EP4029881A1 (en) 2010-11-08 2022-07-20 F. Hoffmann-La Roche AG Subcutaneously administered anti-il-6 receptor antibody
BR112015032960B1 (pt) 2013-07-04 2021-01-05 F. Hoffmann-La Roche Ag imunoensaio suprimido por interferência para detectar anticorpos anti-fármaco em amostras de soro
EP3119876A1 (en) 2014-03-19 2017-01-25 Pfizer Inc. Method of cell culture
WO2017051347A2 (en) 2015-09-23 2017-03-30 Pfizer Inc. Cells and method of cell culture
US20220073942A1 (en) 2018-12-06 2022-03-10 Pfizer Inc. Cells with reduced inhibitor production and methods of use thereof

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4657863A (en) * 1982-07-02 1987-04-14 Celanese Corporation Stabilization of a mutant microorganism population
JPH066054B2 (ja) * 1987-10-15 1994-01-26 帝人株式会社 動物細胞の培養方法
EP0527760B1 (en) * 1990-04-03 1995-07-19 Genentech, Inc. Methods and compositions for vaccination against hiv
GB9022545D0 (en) * 1990-10-17 1990-11-28 Wellcome Found Culture medium
US5252216A (en) * 1992-03-24 1993-10-12 Smithkline Beecham Corporation Protein purification
WO1993022448A1 (en) * 1992-05-01 1993-11-11 Teijin Limited Fed batch culture method for protein secreting cells
GB9506249D0 (en) * 1995-03-27 1995-05-17 Karobio Ab Media for insect cell cultures
US6156570A (en) 1997-03-20 2000-12-05 Regents Of The University Of Minnesota Process for the continuous culture of cells
US6338964B1 (en) * 1999-05-07 2002-01-15 Bayer Corporation Process and medium for mammalian cell culture under low dissolved carbon dioxide concentration

Also Published As

Publication number Publication date
SI2275530T1 (sl) 2019-11-29
PT1585810E (pt) 2010-05-05
US20060127975A1 (en) 2006-06-15
SI1585810T1 (sl) 2010-06-30
DE50312554D1 (de) 2010-05-06
EP2226381A1 (de) 2010-09-08
ES2567161T3 (es) 2016-04-20
JP2010110331A (ja) 2010-05-20
EP2275530A3 (de) 2012-05-23
EP1585810B1 (de) 2010-03-24
EP2275530B1 (de) 2019-08-07
ATE462013T1 (de) 2010-04-15
EP2275530A2 (de) 2011-01-19
DK2226381T3 (en) 2016-04-11
JP4469283B2 (ja) 2010-05-26
EP1585810A1 (de) 2005-10-19
DE10255508A1 (de) 2004-06-17
ES2340377T3 (es) 2010-06-02
EP2226381B1 (de) 2016-03-09
SI2226381T1 (sl) 2016-05-31
US20140329277A1 (en) 2014-11-06
DK1585810T3 (da) 2010-06-07
HUE028862T2 (en) 2017-01-30
JP5221573B2 (ja) 2013-06-26
WO2004048556A1 (de) 2004-06-10
CY1110267T1 (el) 2015-01-14
ES2749217T3 (es) 2020-03-19
JP2006507821A (ja) 2006-03-09

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