CY1107557T1 - Μετασχηματισμενοι μικροοργανισμοι με βελτιωμενες ιδιοτητες - Google Patents

Μετασχηματισμενοι μικροοργανισμοι με βελτιωμενες ιδιοτητες

Info

Publication number
CY1107557T1
CY1107557T1 CY20071100228T CY071100228T CY1107557T1 CY 1107557 T1 CY1107557 T1 CY 1107557T1 CY 20071100228 T CY20071100228 T CY 20071100228T CY 071100228 T CY071100228 T CY 071100228T CY 1107557 T1 CY1107557 T1 CY 1107557T1
Authority
CY
Cyprus
Prior art keywords
substrates
organizations
improved properties
relates
products
Prior art date
Application number
CY20071100228T
Other languages
English (en)
Inventor
Aristos Aristidou
John Londesborough
Merja Penttilä
Peter Richard
Laura Ruohonen
Hans SÖDERLUND
Anita Teleman
Mervi Toivari
Original Assignee
Valtion Teknillinen Tutkimuskeskus
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Valtion Teknillinen Tutkimuskeskus filed Critical Valtion Teknillinen Tutkimuskeskus
Publication of CY1107557T1 publication Critical patent/CY1107557T1/el

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0012—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7)
    • C12N9/0014—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7) acting on the CH-NH2 group of donors (1.4)
    • C12N9/0016—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7) acting on the CH-NH2 group of donors (1.4) with NAD or NADP as acceptor (1.4.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0006—Oxidoreductases (1.) acting on CH-OH groups as donors (1.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1025—Acyltransferases (2.3)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1205—Phosphotransferases with an alcohol group as acceptor (2.7.1), e.g. protein kinases
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P13/00—Preparation of nitrogen-containing organic compounds
    • C12P13/04—Alpha- or beta- amino acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P13/00—Preparation of nitrogen-containing organic compounds
    • C12P13/04—Alpha- or beta- amino acids
    • C12P13/08—Lysine; Diaminopimelic acid; Threonine; Valine
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
    • C12P7/06—Ethanol, i.e. non-beverage
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
    • C12P7/18—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic polyhydric
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/62—Carboxylic acid esters
    • C12P7/625—Polyesters of hydroxy carboxylic acids
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00—Technologies for the production of fuel of non-fossil origin
    • Y02E50/10—Biofuels, e.g. bio-diesel

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)
  • Prevention Of Electric Corrosion (AREA)

Abstract

Η παρούσα εφεύρεση αναφέρεται στη γενετική τεχνητή διαμόρφωση μικροοργανισμών παραγωγής που χρησιμοποιούνται στην βιοτεχνολογία για τη βελτίωση των ικανοτήτων τους έτσι ώστε να παράγουν χρήσιμα προϊόντα πιο αποτελεσματικά. Οι μικροοργανισμοί εκφράζουν τουλάχιστον ένα ένζυμο το οποίο προκαλεί τη λειτουργική σύζευξη της οξείδωσης και αναγωγής των υποστρωμάτων με δυο αντιδράσεις δεϋδρογενάσης συνδεδεμένης με νουκλεοτίδιο πυριδίνης με διαφορετικές ειδικότητες για τα ζεύγη συνενζύμων NAD/NADH και NADP/NADPH και κατ' αυτόν τον τρόπο διευκολύνεται η μεταφορά ηλεκτρονίων ανάμεσα στα δυο ζεύγη συνενζύμων μέσω των αναφερθέντων υποστρωμάτων. Ειδικότερα η εφεύρεση αναφέρεται στην αύξηση των αποδόσεων προϊόντων όπως αιθανόλη ή αμινοξέα από πηγές άνθρακα και αζώτου όπως βιομάζα που περιέχει εξόζες, πεντόζες ή τα πολυμερή αυτών.
CY20071100228T 1998-03-11 2007-02-20 Μετασχηματισμενοι μικροοργανισμοι με βελτιωμενες ιδιοτητες CY1107557T1 (el)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
FI980551A FI980551A (fi) 1998-03-11 1998-03-11 Transformoidut mikro-organismit, joilla on parannettuja ominaisuuksia
EP99907641A EP0981600B1 (en) 1998-03-11 1999-03-11 Transformed microorganisms with improved properties

Publications (1)

Publication Number Publication Date
CY1107557T1 true CY1107557T1 (el) 2013-03-13

Family

ID=8551228

Family Applications (1)

Application Number Title Priority Date Filing Date
CY20071100228T CY1107557T1 (el) 1998-03-11 2007-02-20 Μετασχηματισμενοι μικροοργανισμοι με βελτιωμενες ιδιοτητες

Country Status (13)

Country Link
US (1) US7091014B1 (el)
EP (1) EP0981600B1 (el)
JP (1) JP4573365B2 (el)
AT (1) ATE348144T1 (el)
AU (1) AU756211B2 (el)
CA (1) CA2289023A1 (el)
CY (1) CY1107557T1 (el)
DE (1) DE69934366T2 (el)
DK (1) DK0981600T3 (el)
ES (1) ES2274619T3 (el)
FI (1) FI980551A (el)
PT (1) PT981600E (el)
WO (1) WO1999046363A1 (el)

Families Citing this family (22)

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US7226761B2 (en) 1992-11-05 2007-06-05 Danisco Sweeteners Oy Manufacture of five-carbon sugars and sugar alcohols
WO2000003021A2 (en) * 1998-07-10 2000-01-20 Jens Nielsen Metabolically engineered microbial cell comprising a modified redox activity
DE19907347A1 (de) 1999-02-20 2000-08-24 Degussa Verfahren zur fermentativen Herstellung von L-Aminosäuren unter Verwendung coryneformer Bakterien
ATE263246T1 (de) * 1999-02-20 2004-04-15 Degussa Verfahren zur fermentativen herstellung von l- aminosäuren unter verwendung coryneformer bakterien
JP3965821B2 (ja) * 1999-03-09 2007-08-29 味の素株式会社 Ｌ−リジンの製造法
JP4380029B2 (ja) * 2000-07-05 2009-12-09 味の素株式会社 微生物を利用した物質の製造法
FI20010308A0 (fi) * 2001-02-16 2001-02-16 Valtion Teknillinen Sienen käsitteleminen geneettisesti siten, että se pystyy käyttämään L-arabinoosia
FI20012091A0 (fi) * 2001-10-29 2001-10-29 Valtion Teknillinen Sienimikro-organismi, jolla on parantunut suorituskyky bioteknisessä prosesseissa
DE10240603A1 (de) * 2002-09-03 2004-03-11 Degussa Ag Verwendung von Malat-Dehydrogenase zur NADH-Regenerierung
DK1664318T3 (da) * 2004-01-30 2010-01-25 Ajinomoto Kk L-aminosyre-producerende mikroorganisme og fremgangsmåde til fremstilling af L-aminosyre
US8003367B2 (en) * 2004-03-16 2011-08-23 Ajinomoto Co., Inc. Method for producing L-amino acids by fermentation using bacteria having enhanced expression of xylose utilization genes
PL2236613T3 (pl) * 2004-07-15 2015-11-30 Dsm Ip Assets Bv Biochemiczna synteza 1,4-butanodiaminy
US20080293101A1 (en) * 2006-07-27 2008-11-27 Peters Matthew W Engineered microorganisms for increasing product yield in biotransformations, related methods and systems
CN101978067A (zh) 2008-01-25 2011-02-16 诺维信公司 在醛脱氢酶存在下产生发酵产物
EP2255004B1 (en) * 2008-02-20 2014-04-30 Nagarjuna Fertilizers And Chemicals Limited Genetically transformed microorganism for biomass fermentation
WO2009158627A2 (en) 2008-06-27 2009-12-30 Edeniq, Inc. Cellulosic protein expression in yeast
US20100143997A1 (en) 2008-10-31 2010-06-10 Thomas Buelter Engineered microorganisms capable of producing target compounds under anaerobic conditions
US8198057B2 (en) * 2009-06-08 2012-06-12 Alternative Green Technologies, Llc Ethanol production by fermentation of chinese tallow tree
US11319558B2 (en) * 2009-10-28 2022-05-03 Province Brands Pichia stipitis strain, methods of using the same, and method of isolating a pichia stipitis strain
WO2011055626A1 (en) * 2009-11-06 2011-05-12 Semiconductor Energy Laboratory Co., Ltd. Semiconductor device
CN103314101B (zh) 2010-11-03 2016-05-18 加利福尼亚大学董事会 通过蛋白质生物质发酵的重组微生物的生物燃料和化学生产
CN113717872A (zh) * 2021-09-27 2021-11-30 河北省微生物研究所有限公司 一种筛选高表达fad-gdh重组毕赤酵母菌株的方法

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US4511656A (en) * 1981-05-15 1985-04-16 Purdue Research Foundation Direct fermentation of D-xylose to ethanol by a xylose-fermenting yeast mutant
US5487989A (en) * 1988-08-31 1996-01-30 Bioenergy International, L.C. Ethanol production by recombinant hosts
US5424202A (en) * 1988-08-31 1995-06-13 The University Of Florida Ethanol production by recombinant hosts
FI901771A (fi) * 1990-04-06 1991-10-07 Valtion Teknillinen Anvaendning av xylos hos hybridjaest.
CA2175042C (en) 1993-10-28 2002-05-28 Hiroyuki Kojima Method for production of substances using microorganisms with an increased productivity for nadph
US5789210A (en) * 1993-11-08 1998-08-04 Purdue Research Foundation Recombinant yeasts for effective fermentation of glucose and xylose
IN191596B (el) 1996-05-06 2003-12-06 Purdue Research Foundation

Also Published As

Publication number Publication date
JP2001525682A (ja) 2001-12-11
ATE348144T1 (de) 2007-01-15
WO1999046363A1 (en) 1999-09-16
AU2730399A (en) 1999-09-27
JP4573365B2 (ja) 2010-11-04
FI980551A (fi) 1999-09-12
ES2274619T3 (es) 2007-05-16
CA2289023A1 (en) 1999-09-16
PT981600E (pt) 2007-02-28
DE69934366T2 (de) 2007-10-25
AU756211B2 (en) 2003-01-09
EP0981600A1 (en) 2000-03-01
US7091014B1 (en) 2006-08-15
FI980551A0 (fi) 1998-03-11
DK0981600T3 (da) 2007-02-26
EP0981600B1 (en) 2006-12-13
DE69934366D1 (de) 2007-01-25

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