CS223685B1 - Method of electrophoretic separation of the mixture of anorganic ions by means of the n-/methylphosphone/iminodivinegar acid - Google Patents
Method of electrophoretic separation of the mixture of anorganic ions by means of the n-/methylphosphone/iminodivinegar acid Download PDFInfo
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- CS223685B1 CS223685B1 CS817671A CS767181A CS223685B1 CS 223685 B1 CS223685 B1 CS 223685B1 CS 817671 A CS817671 A CS 817671A CS 767181 A CS767181 A CS 767181A CS 223685 B1 CS223685 B1 CS 223685B1
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Abstract
Vynález spadá do odboru analytických separačných metod. Rieši spósob elektroforeticke] separácie zmesi anorganických iontov pomocou kyseliny N-(metylfosfónovej)- iminodioctovej. Postupuje sa tým spósobom, že zmes katiónov zo skupiny Fe(III)—Cu— i —Ni—Co—Mn—Mg a Mn—Fe(III)—La—Ce nanesená na papieri, s výhodou vo formě 0,05 M roztoku jednotlivých katiónov sa e/ lektroforeticky dělí pri potenciálovom spá de 15 V/cm a teplote 20 °C v základnom e·- lektrolyte, tvorenom roztokom kyseliny N-(metylfosfónovej)iminodioctovej, s výhodou 0,02 M, upravenej na pH 2,9 sústavou tlmivých roztokov.The invention is within the scope of analytical separation methods. Resolved electrophoretic] separating the mixture of inorganic ions with N- (methylphosphonic) acid - iminodiacetic. This is the way that a mixture of cations of the Fe (III) - Cu group i —Ni — Co — Mn — Mg and Mn — Fe (III) —La — Ce deposited on paper, preferably in a mold The 0.05 M solution of the individual cations is e / lectrophoretically divides at potential sleep 15 V / cm and 20 ° C in the base e · - electrolyte, consisting of a solution of N- (methylphosphonic) iminodiacetic acid, preferably 0.02 M, adjusted to pH 2.9 with a buffer system solutions.
Description
Vynález spadá do odboru analytických separačných metod. Rieši spósob elektroforeticke] separácie zmesi anorganických iontov pomocou kyseliny N-( metylf osfónovej)iminodioctovej. Postupuje sa tým spósobom, že zmes katiónov zo skupiny Fe(III)—Cu— i —Ni—Co—Mn—Mg a Mn—Fe(III)— La—Ce nanesená na papieri, s výhodou vo formě 0,05 M roztoku jednotlivých katiónov sa e/ lektroforeticky dělí pri potenciálovom spáde 15 V/cm a teplote 20 °C v základnom e·lektrolyte, tvorenom roztokom kyseliny N-(metylf osf ónove j) iminodioctovej, s výhodou 0,02 M, upravenej na pH 2,9 sústavou tlmivých roztokov.The invention falls within the field of analytical separation methods. It solves the method of electrophoretic separation of a mixture of inorganic ions using N- (methylphosphonic) iminodiacetic acid. The procedure is such that a mixture of cations of the group Fe (III) - Cu - Ni - Co - Mn - Mg and Mn - Fe (III) - La - Ce deposited on paper, preferably in the form of a 0.05 M solution the individual cations are electrophoretically separated at a potential drop of 15 V / cm and a temperature of 20 ° C in a basic electrolyte consisting of a solution of N- (methylphosphonium) iminodiacetic acid, preferably 0.02 M, adjusted to pH 2, 9 system of buffers.
Vynález sa týká sposobu elektroforetickej separácie zmesi anorganických iontov pomocou kyseliny N-(metylf osf óinovejj-iminodioctovej H2O3P—CH2—N (CH2—COOH )2.The present invention relates to a method of electrophoretic separation of a mixture of inorganic ions with N- (methylphosphinino-iminodiacetic acid) H 2 O 3 P-CH 2 -N (CH 2 -COOH) 2 .
V súčasnosti je známe velké množstvo komplexotvorných činidiel, ktoré nie vždy uspokojujú požiadavky selektivnej chelatácie fyzikálně chemicky příbuzných katiónov. Niektoré z nich sa používajú aj pre eloktroforetické delenie zmesi anorganických iontov [Jokl. V., Undentch M., Majer J., J. Chromatog. 26/1, 208 (1967) a Maslovska J., Orzelouski R.: Chem. Anal., 21, 21(1), 219 (1976)], ako například kyselina hydroxy-etyl-iminodioctová. Nevýhodou ich použitia je však dlhý čas potřebný na rozdelenie jednotlivých zložiek zmesi anorganických ionov.A large number of complexing agents are currently known which do not always satisfy the selective chelation requirements of physically chemically related cations. Some of them are also used for electrophoretic separation of a mixture of inorganic ions [Jokl. V., Undentch M., Majer J., J. Chromatog. 26/1, 208 (1967) and Maslovska J., Orzelouski R. Chem. Anal., 21, 21 (1), 219 (1976)], such as hydroxyethyl iminodiacetic acid. However, their disadvantage is the long time required to separate the individual components of the inorganic ion mixture.
Uvedený nedostatok je odstránený u sposobu elektroforetickej separácie zmesi anorganických iontov pomocou kyseliny N-( metylf osf ónove j) iminodioctovej, podía vynálezu, ktorého podstata je, že zmes katiónov zo skupiny Fe(III)—Cu—Ni—Co—Mn—Mg a Mn— —Fe(III)—La—Ce nanesená na papieri, s výhodou vo formě roztoku s koncentráciou jednotlivých katiónov 0,05 M sa elektroforeticky. dělí pri potenciálovom spáde 15 V/cm a teplote 20 °C v základnom elektrolyte, tvorenom roztokom kyseliny N-( metylf osf ónovej iminodioctovej s výhodou 0,02 M, upravenej na pH 2,9 sústavou tlmivých roztokov.This drawback is overcome by the method of electrophoretic separation of a mixture of inorganic ions with N- (methylphosphonic) iminodiacetic acid, according to the invention, which is based on a mixture of cations of the group Fe (III) -Cu-Ni-Co-Mn-Mg and Mn-Fe (III) -La-Ce deposited on paper, preferably in the form of a solution with a single cation concentration of 0.05 M, is electrophoretically applied. It separates at a potential drop of 15 V / cm and a temperature of 20 ° C in the base electrolyte consisting of a solution of N- (methylphosphonium iminodiacetic acid, preferably 0.02 M, adjusted to pH 2.9 with a buffer system).
Výhodou postupu podía vynálezu je, že umožňuje selektívne delenie uvedených zmesi katiónov za poměrně krátký čas. Spósobom podía vynálezu sa dělí s velmi dobrým ofektom zmes Co—Mn—Mg. Výhodou komplexotvorného činidla je aj jeho dobrá rozpustnost vo vodě, a to aj v kyslom prostředí. Skvrny všetkých zložiek dělených zmesi sú ostro ohraničené a takto detegrovateíné.An advantage of the process according to the invention is that it allows the selective separation of said cation mixtures in a relatively short time. According to the process of the invention, a mixture of Co-Mn-Mg is separated with a very good off-rate. The advantage of the complexing agent is its good solubility in water, even under acidic conditions. The stains of all components of the divided mixture are sharply delimited and thus detectable.
Postup podía vynálezu je demonštrovaný na príkladoch prevedenia a obrázku.The process of the invention is demonstrated by way of example and figure.
Příklad 1 ! - nExample 1! - n
Na chromatografický papier Whatman č. 2 rozmerov 14 x 23 cm navlhčený základným elektrolytom tvořeným 0,02 M roztokom kyseliny N-( metylf osf ónove j) iminodioctovej (iontová sila I = 0,1) upraveným na pH 2,9 pomocou tlmivého roztoku bola nanesená zmes katiónov Fe(III)—Cu—Ni— —Co—Mn—Mg, upravená tak, aby výsledná koncentrácia zložie«k bola 0,05 M. Elektroforéza bola uskutočnená vo vodou temperovanej aparatúre bez elektrodových nádobiek pri potenciálovom spáde 15 V/cm, pri teplote 20 °C, počas 60 minút. Po skončení bola detekcia uskutočnená běžnými detekčnými činidlami. Vzdialenosti jednotlivých zložiek dělených zmesi sú zobrazené na obr.On Whatman No. 2 chromatography paper, 14 x 23 cm, moistened with a 0.02 M basic electrolyte consisting of N- (methylphosphonium) iminodiacetic acid (ionic strength I = 0.1) adjusted to pH 2.9 with buffer was deposited mixture of Fe (III) - Cu - Ni - Co - Mn - Mg cations, adjusted to a final composition concentration k of 0.05 M. Electrophoresis was performed in a water tempered apparatus without electrode vessels at a potential drop of 15 V / cm, at 20 ° C, for 60 minutes. After completion, detection was performed with conventional detection reagents. The distances of the individual components of the divided mixture are shown in FIG.
Příklad 2Example 2
Delonie zmesi Mn—Fe(III)—La—Ce, postupom podía příkladu 1 je znázorněné na obr. 1.The delony of the Mn-Fe (III) -La-Ce mixture, as described in Example 1, is shown in Figure 1.
Claims (1)
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Application Number | Priority Date | Filing Date | Title |
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CS817671A CS223685B1 (en) | 1980-05-04 | 1981-10-20 | Method of electrophoretic separation of the mixture of anorganic ions by means of the n-/methylphosphone/iminodivinegar acid |
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CS307280A CS223658B1 (en) | 1980-05-04 | 1980-05-04 | Method of electrostatic separation of the mixture of anorganic ions by means of the n-/methylphosphone/iminodiacetic acid |
CS817671A CS223685B1 (en) | 1980-05-04 | 1981-10-20 | Method of electrophoretic separation of the mixture of anorganic ions by means of the n-/methylphosphone/iminodivinegar acid |
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CS223685B1 true CS223685B1 (en) | 1983-11-25 |
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CS307280A CS223658B1 (en) | 1980-05-04 | 1980-05-04 | Method of electrostatic separation of the mixture of anorganic ions by means of the n-/methylphosphone/iminodiacetic acid |
CS817669A CS223683B1 (en) | 1980-05-04 | 1981-10-20 | Method of electrophoretic separation of the mixture of anorganic ions by means of the glycine-n,n-bis/methylphosphone acid |
CS817670A CS223684B1 (en) | 1980-05-04 | 1981-10-20 | Method of electrophoretic separation of mixture of anorganic ions by means of the glycine-n,n-bis/methylphosphone/acid |
CS817671A CS223685B1 (en) | 1980-05-04 | 1981-10-20 | Method of electrophoretic separation of the mixture of anorganic ions by means of the n-/methylphosphone/iminodivinegar acid |
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CS307280A CS223658B1 (en) | 1980-05-04 | 1980-05-04 | Method of electrostatic separation of the mixture of anorganic ions by means of the n-/methylphosphone/iminodiacetic acid |
CS817669A CS223683B1 (en) | 1980-05-04 | 1981-10-20 | Method of electrophoretic separation of the mixture of anorganic ions by means of the glycine-n,n-bis/methylphosphone acid |
CS817670A CS223684B1 (en) | 1980-05-04 | 1981-10-20 | Method of electrophoretic separation of mixture of anorganic ions by means of the glycine-n,n-bis/methylphosphone/acid |
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1980
- 1980-05-04 CS CS307280A patent/CS223658B1/en unknown
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1981
- 1981-10-20 CS CS817669A patent/CS223683B1/en unknown
- 1981-10-20 CS CS817670A patent/CS223684B1/en unknown
- 1981-10-20 CS CS817671A patent/CS223685B1/en unknown
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CS223684B1 (en) | 1983-11-25 |
CS223683B1 (en) | 1983-11-25 |
CS223658B1 (en) | 1983-11-25 |
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