CO4930292A1 - Proteinas cry 1c modificada para la expresion en plantas de 8-endotoxinas activas contra lepidopteros, composiciones que comprenden dichas proteinas y metodo para producirla - Google Patents

Proteinas cry 1c modificada para la expresion en plantas de 8-endotoxinas activas contra lepidopteros, composiciones que comprenden dichas proteinas y metodo para producirla

Info

Publication number
CO4930292A1
CO4930292A1 CO97069522A CO97069522A CO4930292A1 CO 4930292 A1 CO4930292 A1 CO 4930292A1 CO 97069522 A CO97069522 A CO 97069522A CO 97069522 A CO97069522 A CO 97069522A CO 4930292 A1 CO4930292 A1 CO 4930292A1
Authority
CO
Colombia
Prior art keywords
seq
polypeptide
proteins
amino acid
produce
Prior art date
Application number
CO97069522A
Other languages
English (en)
Inventor
James A Baum
Gilmer Amy Jelen
Light Anne-Marie Mettus
Original Assignee
Ecogen Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Ecogen Inc filed Critical Ecogen Inc
Publication of CO4930292A1 publication Critical patent/CO4930292A1/es

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C12N1/205—Bacterial isolates
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B41—PRINTING; LINING MACHINES; TYPEWRITERS; STAMPS
    • B41M—PRINTING, DUPLICATING, MARKING, OR COPYING PROCESSES; COLOUR PRINTING
    • B41M5/00—Duplicating or marking methods; Sheet materials for use therein
    • B41M5/26—Thermography ; Marking by high energetic means, e.g. laser otherwise than by burning, and characterised by the material used
    • B41M5/382—Contact thermal transfer or sublimation processes
    • B41M5/392—Additives, other than colour forming substances, dyes or pigments, e.g. sensitisers, transfer promoting agents
    • B41M5/395—Macromolecular additives, e.g. binders
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/32—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Bacillus (G)
    • C07K14/325—Bacillus thuringiensis crystal peptides, i.e. delta-endotoxins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8279—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance
    • C12N15/8286—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance for insect resistance
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C12R2001/07—Bacillus
    • C12R2001/075—Bacillus thuringiensis
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
    • Y02A40/146—Genetically Modified [GMO] plants, e.g. transgenic plants
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S428/00—Stock material or miscellaneous articles
    • Y10S428/913—Material designed to be responsive to temperature, light, moisture
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S428/00—Stock material or miscellaneous articles
    • Y10S428/914—Transfer or decalcomania
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T428/00—Stock material or miscellaneous articles
    • Y10T428/31504—Composite [nonstructural laminate]
    • Y10T428/31855—Of addition polymer from unsaturated monomers

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Microbiology (AREA)
  • Pest Control & Pesticides (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Virology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Insects & Arthropods (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Cell Biology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Plant Pathology (AREA)
  • Optics & Photonics (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

Un segmento de ácidos nucleicos aislado que codifica unpolipéptido, CARACTERIZADO PORQUE comprende la secuencia de aminoácidos de la SEQ ID N°. 2, SEQ ID N°. 4, SEQ ID N°. 6, SEQ ID N°. 8, SEQ ID N°. 10 o SEQ ID N°. 12.Una composición, CARACTERIZADA PORQUE contiene un polipéptido aislado que comprende la secuencia de aminoácidos de la SEQ ID N°. 2, SEQ ID N°. 4, SEQ ID N°. 8. SEQ ID N°. 10 o SEQ ID N°. 12. 5Un método para mutar un polipéptido Cryl de acuerdo con cualquiera de las reivindicaciones precedentes para aumentar la actividad insecticida de dicho polipéptido contra un insecto Lepidóptero, CARACTERIZADO PORQUE dicho método comprende los siguientes pasos:(a) predecir en dicho polipéptido una secuencia contigua de aminoácidos que codifican una región de bucle entre a-hélices adyacentes del dominio 1 o entre una a-hélice del dominio 1 y una cadena ß del domino 2; (b) mutar uno o más de dichos residuos de aminoácidos en dicha secuencia contigua de aminoácidos con el fin de producir una población de polipéptidos que posea una o más regiones de bucle alteradas.(c) probar la actividad insecticida contra dicho insecto Lepidóptero de dicha población de polipéptidos, e (d) identificar en dicha población un polipéptido que posea una mayor actividad insecticida contra dicho insecto Lepidóptero.
CO97069522A 1996-11-27 1997-11-27 Proteinas cry 1c modificada para la expresion en plantas de 8-endotoxinas activas contra lepidopteros, composiciones que comprenden dichas proteinas y metodo para producirla CO4930292A1 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US08/757,536 US5942664A (en) 1996-11-27 1996-11-27 Bacillus thuringiensis Cry1C compositions toxic to lepidopteran insects and methods for making Cry1C mutants

Publications (1)

Publication Number Publication Date
CO4930292A1 true CO4930292A1 (es) 2000-06-27

Family

ID=25048194

Family Applications (1)

Application Number Title Priority Date Filing Date
CO97069522A CO4930292A1 (es) 1996-11-27 1997-11-27 Proteinas cry 1c modificada para la expresion en plantas de 8-endotoxinas activas contra lepidopteros, composiciones que comprenden dichas proteinas y metodo para producirla

Country Status (17)

Country Link
US (11) US5942664A (es)
EP (1) EP0942929A1 (es)
JP (1) JP2001506490A (es)
CN (1) CN1245502A (es)
AP (1) AP9901542A0 (es)
AR (1) AR010662A1 (es)
AU (1) AU745431B2 (es)
BR (1) BR9713555A (es)
CA (1) CA2272847A1 (es)
CO (1) CO4930292A1 (es)
EA (1) EA199900503A1 (es)
ID (1) ID23695A (es)
IL (1) IL130082A0 (es)
OA (1) OA11258A (es)
TR (1) TR199901179T2 (es)
WO (1) WO1998023641A1 (es)
ZA (1) ZA9710431B (es)

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