CO2022003888A2 - Sistemas y métodos de uso y regeneración de cromatografía - Google Patents
Sistemas y métodos de uso y regeneración de cromatografíaInfo
- Publication number
- CO2022003888A2 CO2022003888A2 CONC2022/0003888A CO2022003888A CO2022003888A2 CO 2022003888 A2 CO2022003888 A2 CO 2022003888A2 CO 2022003888 A CO2022003888 A CO 2022003888A CO 2022003888 A2 CO2022003888 A2 CO 2022003888A2
- Authority
- CO
- Colombia
- Prior art keywords
- alkaline solution
- hydrophobic interaction
- regeneration
- chromatography
- systems
- Prior art date
Links
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/26—Conditioning of the fluid carrier; Flow patterns
- G01N30/38—Flow patterns
- G01N30/40—Flow patterns using back flushing
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
- B01J20/30—Processes for preparing, regenerating, or reactivating
- B01J20/34—Regenerating or reactivating
- B01J20/345—Regenerating or reactivating using a particular desorbing compound or mixture
- B01J20/3475—Regenerating or reactivating using a particular desorbing compound or mixture in the liquid phase
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
- B01D15/08—Selective adsorption, e.g. chromatography
- B01D15/10—Selective adsorption, e.g. chromatography characterised by constructional or operational features
- B01D15/20—Selective adsorption, e.g. chromatography characterised by constructional or operational features relating to the conditioning of the sorbent material
- B01D15/203—Equilibration or regeneration
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
- B01D15/08—Selective adsorption, e.g. chromatography
- B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
- B01D15/32—Bonded phase chromatography
- B01D15/325—Reversed phase
- B01D15/327—Reversed phase with hydrophobic interaction
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/16—Extraction; Separation; Purification by chromatography
- C07K1/20—Partition-, reverse-phase or hydrophobic interaction chromatography
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
- C07K16/06—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies from serum
- C07K16/065—Purification, fragmentation
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/50—Conditioning of the sorbent material or stationary liquid
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/88—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/96—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation using ion-exchange
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J2220/00—Aspects relating to sorbent materials
- B01J2220/50—Aspects relating to the use of sorbent or filter aid materials
- B01J2220/58—Use in a single column
Landscapes
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Immunology (AREA)
- Pathology (AREA)
- General Physics & Mathematics (AREA)
- Physics & Mathematics (AREA)
- Organic Chemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Biophysics (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Molecular Biology (AREA)
- Medicinal Chemistry (AREA)
- Genetics & Genomics (AREA)
- Peptides Or Proteins (AREA)
- Treatment Of Liquids With Adsorbents In General (AREA)
- Solid-Sorbent Or Filter-Aiding Compositions (AREA)
Abstract
Aspectos de la presente divulgación se refieren a un método de regeneración de una columna de cromatografía de interacción hidrofóbica a la que se ha aplicado una masa de carga, comprendiendo el método pasar uno o más volúmenes de columna de una solución alcalina a través de medios de interacción hidrofóbica dentro de la columna, donde la solución alcalina presenta un pH de entre aproximadamente 10 y aproximadamente 14, y una conductividad de entre 0,5 mS/cm y aproximadamente 10 mS/cm, donde se elimina el material unido a los medios de interacción hidrofóbica. En algunos casos, la solución alcalina puede incluir hidróxido de sodio a una concentración de entre, por ejemplo, aproximadamente 0,1 mM y 10 mM.
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US201962905033P | 2019-09-24 | 2019-09-24 | |
US202062958899P | 2020-01-09 | 2020-01-09 | |
PCT/US2020/052243 WO2021061790A1 (en) | 2019-09-24 | 2020-09-23 | Systems and methods for chromatography use and regeneration |
Publications (1)
Publication Number | Publication Date |
---|---|
CO2022003888A2 true CO2022003888A2 (es) | 2022-04-19 |
Family
ID=72896066
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CONC2022/0003888A CO2022003888A2 (es) | 2019-09-24 | 2022-03-30 | Sistemas y métodos de uso y regeneración de cromatografía |
Country Status (13)
Country | Link |
---|---|
US (1) | US20220323937A1 (es) |
EP (1) | EP4034870A1 (es) |
JP (1) | JP2022548394A (es) |
KR (1) | KR20220066393A (es) |
CN (1) | CN114667450A (es) |
AU (1) | AU2020352547A1 (es) |
BR (1) | BR112022004885A2 (es) |
CA (2) | CA3150234C (es) |
CL (1) | CL2023003544A1 (es) |
CO (1) | CO2022003888A2 (es) |
IL (1) | IL291020A (es) |
MX (1) | MX2022003438A (es) |
WO (1) | WO2021061790A1 (es) |
Families Citing this family (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2023191827A1 (en) * | 2022-03-28 | 2023-10-05 | President And Fellows Of Harvard College | High efficiency purification of divergent aav serotypes using aavx affinity chromatography |
WO2024102939A1 (en) * | 2022-11-09 | 2024-05-16 | Genzyme Corporation | Composition and methods for cleaning |
Family Cites Families (36)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US7087411B2 (en) | 1999-06-08 | 2006-08-08 | Regeneron Pharmaceuticals, Inc. | Fusion protein capable of binding VEGF |
SG106672A1 (en) | 2002-03-08 | 2004-10-29 | Asml Netherlands Bv | Mask for use in lithography, method of making a mask, lithographic apparatus, and device manufacturing method |
ES2398076T3 (es) | 2006-06-02 | 2013-03-13 | Regeneron Pharmaceuticals, Inc. | Anticuerpos de alta afinidad contra el receptor de IL-6 humano |
US7608693B2 (en) | 2006-10-02 | 2009-10-27 | Regeneron Pharmaceuticals, Inc. | High affinity human antibodies to human IL-4 receptor |
CN101809037B (zh) | 2007-07-31 | 2014-01-15 | 瑞泽恩制药公司 | 人cd20的人抗体及其使用方法 |
JP5498025B2 (ja) * | 2008-03-13 | 2014-05-21 | 公益財団法人相模中央化学研究所 | 新規なチアゾール誘導体固定化マトリックス、及びその製造方法 |
JO3672B1 (ar) | 2008-12-15 | 2020-08-27 | Regeneron Pharma | أجسام مضادة بشرية عالية التفاعل الكيماوي بالنسبة لإنزيم سبتيليسين كنفرتيز بروبروتين / كيكسين نوع 9 (pcsk9). |
JO3417B1 (ar) | 2010-01-08 | 2019-10-20 | Regeneron Pharma | الصيغ المستقرة التي تحتوي على الأجسام المضادة لمضاد مستقبل( interleukin-6 (il-6r |
JO3340B1 (ar) | 2010-05-26 | 2019-03-13 | Regeneron Pharma | مضادات حيوية لـعامل تمايز النمو 8 البشري |
AR083044A1 (es) | 2010-09-27 | 2013-01-30 | Regeneron Pharma | Anticuerpos anti-cd48 y usos de los mismos |
EA028945B1 (ru) | 2010-10-06 | 2018-01-31 | Ридженерон Фармасьютикалз, Инк. | СТАБИЛЬНЫЕ ЖИДКИЕ ФАРМАЦЕВТИЧЕСКИЕ КОМПОЗИЦИИ, СОДЕРЖАЩИЕ АНТИТЕЛА К АЛЬФА РЕЦЕПТОРУ ЧЕЛОВЕЧЕСКОГО ИНТЕРЛЕЙКИНА-4 (hIL-4Rα) |
JO3756B1 (ar) | 2010-11-23 | 2021-01-31 | Regeneron Pharma | اجسام مضادة بشرية لمستقبلات الجلوكاجون |
SG186552A1 (en) * | 2011-06-08 | 2013-01-30 | Emd Millipore Corp | Chromatography matrices including novel staphylococcus aureus protein a based ligands |
AR087329A1 (es) | 2011-06-17 | 2014-03-19 | Regeneron Pharma | Anticuerpos humanos contra proteina 3 de tipo angiopoietina humana |
LT2780368T (lt) | 2011-11-14 | 2018-03-12 | Regeneron Pharmaceuticals, Inc. | Kompozicijos ir būdai raumenų masės padidinimui ir raumenų sustiprinimui, specifiškai antagonizuojant gdf8 ir (arba) aktiviną a |
EA033387B1 (ru) | 2012-01-23 | 2019-10-31 | Regeneron Pharma | СТАБИЛИЗИРОВАННЫЕ СОСТАВЫ, СОДЕРЖАЩИЕ АНТИТЕЛА ПРОТИВ Ang-2 |
US9067990B2 (en) * | 2013-03-14 | 2015-06-30 | Abbvie, Inc. | Protein purification using displacement chromatography |
JO3820B1 (ar) | 2012-05-03 | 2021-01-31 | Regeneron Pharma | أجسام مضادة بشرية لـ fel d1وطرق لاستخدامها |
TWI641619B (zh) | 2012-06-25 | 2018-11-21 | 美商再生元醫藥公司 | 抗-egfr抗體及其用途 |
AU2013302925B2 (en) | 2012-08-13 | 2018-07-05 | Regeneron Pharmaceuticals, Inc. | Anti-PCSK9 antibodies with pH-dependent binding characteristics |
JOP20200236A1 (ar) | 2012-09-21 | 2017-06-16 | Regeneron Pharma | الأجسام المضادة لمضاد cd3 وجزيئات ربط الأنتيجين ثنائية التحديد التي تربط cd3 وcd20 واستخداماتها |
JO3405B1 (ar) | 2013-01-09 | 2019-10-20 | Regeneron Pharma | الأجسام المضادة لمضاد مستقبل عامل النمو المشتق من الصفائح الدموية - بيتا واستخداماتها |
JO3532B1 (ar) | 2013-03-13 | 2020-07-05 | Regeneron Pharma | الأجسام المضادة لمضاد انترلوكين-33 واستعمالاتها |
TWI659968B (zh) | 2013-03-14 | 2019-05-21 | 再生元醫藥公司 | 針對呼吸道融合病毒f蛋白質的人類抗體及其使用方法 |
US9637535B2 (en) | 2013-03-15 | 2017-05-02 | Regeneron Pharmaceuticals, Inc. | IL-33 antagonists and uses thereof |
TWI641620B (zh) | 2013-08-21 | 2018-11-21 | 再生元醫藥公司 | 抗-prlr抗體及其用途 |
JP6602765B2 (ja) * | 2013-09-05 | 2019-11-06 | ジェネンテック, インコーポレイテッド | クロマトグラフィー再使用のための方法 |
TWI681969B (zh) | 2014-01-23 | 2020-01-11 | 美商再生元醫藥公司 | 針對pd-1的人類抗體 |
TWI680138B (zh) | 2014-01-23 | 2019-12-21 | 美商再生元醫藥公司 | 抗pd-l1之人類抗體 |
MA39313B1 (fr) | 2014-03-11 | 2018-12-31 | Regeneron Pharma | Anticorps anti-egfrviii et leurs utilisations dans le traitement de divers cancers |
TWI754319B (zh) | 2014-03-19 | 2022-02-01 | 美商再生元醫藥公司 | 用於腫瘤治療之方法及抗體組成物 |
AU2015256299C1 (en) | 2014-05-05 | 2022-01-06 | Regeneron Pharmaceuticals, Inc. | Humanized C5 and C3 animals |
KR20170062466A (ko) | 2014-09-16 | 2017-06-07 | 리제너론 파마슈티칼스 인코포레이티드 | 항-글루카곤 항체 및 그것의 사용 |
AU2016233557B2 (en) * | 2015-03-13 | 2021-06-24 | Bristol-Myers Squibb Company | Use of alkaline washes during chromatography to remove impurities |
CN104892734B (zh) * | 2015-04-30 | 2019-09-13 | 中国科学院过程工程研究所 | 疏水作用层析提纯***灭活病毒抗原的方法 |
PL3500586T3 (pl) * | 2016-10-17 | 2021-09-27 | Enzene Biosciences Ltd. | Sposób ciągły zmniejszania heterogeniczności białka terapeutycznego |
-
2020
- 2020-09-23 EP EP20792783.1A patent/EP4034870A1/en active Pending
- 2020-09-23 BR BR112022004885A patent/BR112022004885A2/pt unknown
- 2020-09-23 WO PCT/US2020/052243 patent/WO2021061790A1/en unknown
- 2020-09-23 JP JP2022518215A patent/JP2022548394A/ja active Pending
- 2020-09-23 CA CA3150234A patent/CA3150234C/en active Active
- 2020-09-23 CA CA3216345A patent/CA3216345A1/en active Pending
- 2020-09-23 US US17/753,915 patent/US20220323937A1/en active Pending
- 2020-09-23 MX MX2022003438A patent/MX2022003438A/es unknown
- 2020-09-23 CN CN202080074771.9A patent/CN114667450A/zh active Pending
- 2020-09-23 AU AU2020352547A patent/AU2020352547A1/en active Pending
- 2020-09-23 KR KR1020227013765A patent/KR20220066393A/ko unknown
-
2022
- 2022-03-01 IL IL291020A patent/IL291020A/en unknown
- 2022-03-30 CO CONC2022/0003888A patent/CO2022003888A2/es unknown
-
2023
- 2023-11-28 CL CL2023003544A patent/CL2023003544A1/es unknown
Also Published As
Publication number | Publication date |
---|---|
MX2022003438A (es) | 2022-04-19 |
CL2023003544A1 (es) | 2024-04-26 |
CA3216345A1 (en) | 2021-04-01 |
CN114667450A (zh) | 2022-06-24 |
IL291020A (en) | 2022-05-01 |
BR112022004885A2 (pt) | 2022-06-07 |
JP2022548394A (ja) | 2022-11-18 |
EP4034870A1 (en) | 2022-08-03 |
KR20220066393A (ko) | 2022-05-24 |
CA3150234A1 (en) | 2021-04-01 |
AU2020352547A1 (en) | 2022-03-31 |
CA3150234C (en) | 2023-12-12 |
WO2021061790A1 (en) | 2021-04-01 |
US20220323937A1 (en) | 2022-10-13 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
CL2023003544A1 (es) | Sistemas y métodos de uso y regeneración de cromatografía | |
CR8998A (es) | PRODUCCION DE TNFR-Ig Y FUSION DE PROTEINA | |
ES2162621T3 (es) | Dispositivo y procedimiento para detectar analitos. | |
ES2045132T3 (es) | Sensor de dioxido de carbono. | |
DE60303072D1 (de) | Reaktionslösung für Tintenstrahldruckaufzeichnung, Vorrichtung und Tintenstrahlaufzeichnungsverfahren, die diese Lösung verwenden | |
CR8997A (es) | Produccion de polipeptidos | |
DK0431456T3 (da) | Anvendelse af et tungtopløseligt salt af en heteropolysyre til bestemmelse af en analyt, tilsvarende fremgangsmåde til bestemmelsen samt hertil egnet middel | |
SE9801287D0 (sv) | Incorporation of active substances in carrier matrixes | |
ATE227337T1 (de) | Trenn- und nachweisverfahren für spermatozoide | |
ES2125435T3 (es) | Sistema conservador destinado para soluciones para lentes de contacto. | |
BR0313739A (pt) | Método para minimizar a possibilidade de resultados falso-positivos e falso-negativos na detecção de pequenas quantidades de lìquido amniótico em secreção vaginal e dispositivo para realizar o método | |
BR0000068A (pt) | Sistema e método para iniciar a operação de um sistema de computador | |
BRPI0414270A (pt) | dispositivo de remoção de fluxo contìnuo e sistema utilizando o dito dispositivo | |
DK90683D0 (da) | Fremgangsmade til udvinding af insulin fra et insulin-protaminkompleks | |
AR011557A1 (es) | Un medio de cultivo y metodos para detectar estafilococos | |
DK1329461T3 (da) | Fremgangsmåde til fjernelse af humane serum-albumin-polymerer | |
ES2179500T3 (es) | Recipiente para tinta configurado para uso con un dispositivo de impresion que tiene un sistema detector del agotamiento de la tinta. | |
ATE534031T1 (de) | Verfahren und vorrichtung für die chemische regenerierung mit verschiebung | |
AR063751A1 (es) | Metodos para evaluar las velocidades de difusion de sistemas de administracion de farmacos oculares | |
FR2253615A1 (es) | ||
JPS52129582A (en) | Flaw detector | |
DE69221153T2 (de) | Kationselektive Elektroden | |
ES2077357T3 (es) | Separacion de particulas de sulfuro de hierro a partir de soluciones de alcanolaminas. | |
AR047461A1 (es) | Formacion, purificacion y uso de n-formilolanzapina | |
UY25071A1 (es) | Composiciones solubilizadas de sertralina |