CN2833595Y - Colloid gold test paper for quick detection of metalaxyl residue - Google Patents
Colloid gold test paper for quick detection of metalaxyl residue Download PDFInfo
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- CN2833595Y CN2833595Y CN 200520106172 CN200520106172U CN2833595Y CN 2833595 Y CN2833595 Y CN 2833595Y CN 200520106172 CN200520106172 CN 200520106172 CN 200520106172 U CN200520106172 U CN 200520106172U CN 2833595 Y CN2833595 Y CN 2833595Y
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- metalaxyl
- test paper
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Abstract
The utility model relates to a piece of colloidal gold test paper for rapidly detecting metalaxyl residues, which is composed of a bottom plate, a water absorbing plate, a nitrocellulose film, a metalaxyl monoclonal antibody gold label pad and a sample liquid absorbing layer, wherein the middle part of the bottom plate is provided with the nitrocellulose film. A metalaxyl-BSA bond test line and a polyclonal antibody control line are arranged on the nitrocellulose film. The end head at one end of the bottom is provided with the water absorbing plate, and the end head at the other end is provided with the sample liquid absorbing layer. Both ends of the nitrocellulose film are respectively and mutually overlapped and connected with the water absorbing plate and the metalaxyl monoclonal antibody gold label pad. The sample liquid absorbing layer is pressed on the metalaxyl monoclonal antibody gold label pad. The test paper is made through the immunization compete method of colloidal gold. The immunization colloidal gold method is used for detecting whether the residual quantity of metalaxyl in fruit vegetable is overproof or not. The method has the advantages of rapidity, sensitivity, convenient operation and low cost. The utility model does not need to be detected by professionals, and the organic unification of complicated theory and simple operation can be really achieved. Simultaneously, the utility model has the characteristics of convenient preservation and long effective period.
Description
Technical field
The present invention relates to a kind of fruits and vegetables and raw grain based food metalaxyl residue detection test paper and detection method thereof of being used for, belong to the colloid gold immune detection range.
Background technology
Because the vegetable growth phase is short and disease and pest is comparatively serious, thereby, in the growing vegetables process, usually need the continuous several times dispenser.And many vegetables need repeatedly be gathered, gather and the time interval of dispenser short, cause when gathering the persticide residue in the vegetables often higher, be easy to surpass state specified standards.And persticide residue exceeds standard and can have a strong impact on level of human health in the vegetables, has become the food pollution source that can not be ignored.Government and each functional department extremely pay close attention to the residues of pesticides problem of food.But because the resistance to the action of a drug of the toxicity of agricultural chemicals and insect forms a vicious cycle, forbid thoroughly comprehensively that therefore the use of high highly toxic pesticide will have long process, even in using, low-toxin farm chemicals comprehensively, the pernicious poisoning of agricultural chemicals still takes place.Therefore new requirement and higher standard have all been proposed all many-sides such as the object of analyzing and testing, kind, quantity, scope, index.
Metalaxyl (metalaxyl; D; L-N-(2; the 6-3,5-dimethylphenyl)-N-(2 '-methoxyl acetyl) methyl lactamine); belonging to phenylamide, is a kind of systemic fungicide as protection and therapeutic action, can and be transmitted to inside plants by seed, root, stem, leaf absorption; be applicable to prevention and treatment, be mainly used under the various weather conditions of control by the fungus-caused disease of Peronosporales by air and soil belt fungus diseases.As the late blight of potato, downy mildew of garpe, tobacco downy mildew, hop downy mildew and downy mildew of lettuce.Though metalaxyl is not high to people and animals' toxicity, absorption sterilization in belonging to, the situation that exceeds standard of the residual quantity seasonality in food and vegetables is more, and poisoning often has generation.In view of the foregoing, metalaxyl has been listed in the important detected object of residues of pesticides.
At present, the analytical approach of detection residues of pesticides mainly is dependence, vapor-phase chromatography (GC) or gas chromatography and mass spectrum (MS) coupling method (GC/MS)." enzyme inhibition rate method+spectrophotometric method " has been listed in national proposed standard (GB/T 5009.199-2003).This method is quick, sensitive, easy and simple to handle and with low cost, can realize quick primary dcreening operation, but the restriction of examined scope and precision.And vapor-phase chromatography (GC) can realize the quantitative test of precision but speed is slower, and it is higher to detect cost.In a word, these method ubiquities the sample pretreatment process complexity, sensitivity be subjected to sample purification, step such as concentrate influence very big, consuming time, checkout equipment costliness, and require to have technical professional and long analytical cycle.
Therefore, utilize the competition law principle, on the basis of traditional immunodetection, introduced the collaurum Fast Detection Technique, develop metalaxyl collaurum competition law and detect test paper.This method has fast, sensitive, easy and simple to handle, cost is low, need not the professional detects, and really reaches the organic unity of complicated principle and ease of Use, have simultaneously preserve convenient, the characteristics that the term of validity is long.
Summary of the invention
The present invention is directed to some problems of above-mentioned existence, provide a kind of being applicable to detect the collaurum mensuration test paper whether metalaxyl exceeds standard.
For solving the problems of the technologies described above, the present invention is achieved by the following technical solutions:
Detecting test paper base plate middle part at metalaxyl residue is nitrocellulose filter, a test wire and a sheep anti mouse polyclonal antibody control line are arranged on the nitrocellulose filter, in base plate one end termination is water accepting layer, other end termination is the sample liquid-adsorption layer, the nitrocellulose filter two ends overlap mutually with metalaxyl monoclonal antibody gold mark pad with water accepting layer respectively and are connected (the overlapping coupling part is in 1-2 millimeter scope), are pressed with the sample liquid-adsorption layer on metalaxyl monoclonal antibody gold mark pad.Combined method about base plate, nitrocellulose filter, thieving paper, collaurum mark pad has been known technology.Wanhuapuman Bioengineering Co Ltd obtained the traditional Chinese medicines word authentication code that competition law detects the morphine test paper in 1998: the accurate word S19990038 of traditional Chinese medicines number, and with launch products.
The control line that metalaxyl residue detects test paper is formed by sheep anti mouse polyclonal antibody bag, and test wire is to wrap quilt by metalaxyl-BSA.The sample liquid-adsorption layer end of the colloid gold test paper that detects metalaxyl residue is put into Juice, because the capillarity sample will move along test strips water accepting layer end, when moving to metalaxyl monoclonal antibody gold mark pad, metalaxyl in the sample and metalaxyl monoclonal antibody gold mark probe generation specific bond, when moving to when being fixed with metalaxyl-BSA and connecting the thing test wire, lose it and combine owing to metalaxyl in the antibody in the metalaxyl monoclonal antibody gold mark pad and the sample preferentially is combined into compound with metalaxyl-BSA, therefore its collaurum can not be stranded on the test wire, the test wire place does not have red line to show, promptly has only a red control line positive; If do not have metalaxyl in the opposite sample, antibody in the metalaxyl monoclonal antibody gold mark pad moves on the test wire, the metalaxyl monoclonal antibody will connect thing generation specific bond with metalaxyl-BSA, collaurum is stranded on the test wire, promptly two red line are negative, immune competition law principle that Here it is.Metalaxyl content just is inversely proportional in the shade of test wire and the sample.Carry out the transition to feminine gender from the positive, because the metalaxyl content difference of surveying, the color of test wire is also different, forms a red color gradient difference, detects the OD value with this principle, thereby infers the amount of actual metalaxyl that test wire reacts.
It is 50ng/ml that the limit of identification of test wire can reach metalaxyl content.The garden stuff pesticide residue examination criteria metalaxyl content that the detected value of test wire is set at country's promulgation is that 0.5mg/kg or granule fruit Detecting Pesticide standard metalaxyl content are that 1mg/kg or raw grain Detecting Pesticide standard metalaxyl content are 0.05mg/kg.
It detects more than the metalaxyl setting detected level to make test paper with competition law, and it is positive that a red line appears in the view window place; It is negative to set the two red lines of the following appearance of detected level; Be that fuzzy hacures are boundary value at the test wire place when setting detected level, thereby draw judgement.
When moving to sheep anti mouse polyclonal antibody control line, no matter have or not metalaxyl in the sample, the gold mark probe of mark all can combine delay with the sheep anti mouse polyclonal antibody that has configured, and it is red that control line is shown.Therefore control line does not have colour band and produces that then the representative operation is wrong, during detection the sample liquid level surpass the MAX line or test paper expired.
Because adopt technique scheme, the colloid gold test paper of a kind of fast detecting metalaxyl residue provided by the present invention has such beneficial effect, promptly high specificity is highly sensitive, easily stores, need not the technical skill personnel operation, and readability as a result.
Description of drawings
Fig. 1 is the main TV structure figure of the colloid gold test paper of a kind of fast detecting metalaxyl residue of the present invention.
Fig. 2 is the side-looking structural drawing of invention figure one.
Fig. 3 shows positive findings figure for detecting.
Fig. 4 shows negative findings figure for detecting.
Among the figure 1, water sucting plate, 2, nitrocellulose filter, 3, sheep anti mouse polyclonal antibody control line, 4, test wire, 5, monoclonal antibody gold mark pad, 6, the sample liquid-adsorption layer, 7, base plate, 8, the MAX line.
Specific embodiment
1. utilizing chemical method that the sub-metalaxyl of aliquot is connected synthetic immunogen with big molecule BSA is metalaxyl-BSA.
2. metalaxyl MONOCLONAL ANTIBODIES SPECIFIC FOR
(1) with the immune BALB/c mouse of the metalaxyl-BSA of preparation.Set up the knurl strain with the SP20 Fusion of Cells and screen, get oncocyte and be injected in the BALB/c mouse abdominal cavity, make it produce ascites.
(2) extract the screening of mouse ascites purifying, the monoclonal antibody that obtains to combine with the metalaxyl molecule simultaneously is used for colloid gold label.
The preparation of collaurum and with the combining of metalaxyl monoclonal antibody
(1) get distilled water and add an amount of gold chloride magnetic agitation and be warmed to 90～95 ℃, add an amount of citric acid three and receive and continue heated and stirred to seething with excitement 5 minutes, it is standby to keep in Dark Place after the cooling.
(2) the collaurum liquid of metalaxyl labeling of monoclonal antibody, on the adsorbing fiber material, dry back is standby.
4. film-making machine system film: utilize computer control transmission speed, guarantee that the antibody amount of bag quilt on the per unit film equates.
5. test strips combination: press the known technology combination.
6. using method: the sample liquid-adsorption layer end of test paper is put into Juice (liquid level must not surpass MAX line chart 8), take out test paper after 1 minute and keep flat, because kapillary and syphonic effect sample will move observations in the time of 5 minutes along test strips water accepting layer end.
7. the result judges: detect more than the metalaxyl concentration 0.5mg/kg, it is positive that a red line appears in the view window place; 0.5mg/kg the two red lines of following appearance are negative; 0.5mg/kg the time be that fuzzy hacures are boundary value at the test wire place, this boundary value is the vegetable pesticide residue examination criteria 0.5mg/g of country's promulgation.Thereby draw judgement.
Claims (8)
1. the colloid gold test paper of a fast detecting metalaxyl residue is characterized in that being made up of base plate (7), water sucting plate (1), nitrocellulose filter (2), metalaxyl monoclonal antibody gold mark pad (5), sample liquid-adsorption layer (6); The base plate middle part is a nitrocellulose filter, a test wire (4) and a polyclonal antibody control line (3) are arranged on the nitrocellulose filter, base plate one end termination is a water sucting plate, other end termination is the sample liquid-adsorption layer, the nitrocellulose filter two ends overlap mutually with metalaxyl monoclonal antibody gold mark pad with water sucting plate respectively and are connected, and are pressed with the sample liquid-adsorption layer on metalaxyl monoclonal antibody gold mark pad.
2. the colloid gold test paper of a kind of fast detecting metalaxyl residue according to claim 1 is characterized in that control line is to be made into by sheep anti mouse polyclonal antibody bag.
3. the colloid gold test paper of a kind of fast detecting metalaxyl residue according to claim 1 is characterized in that test wire is to be made into by metalaxyl-BSA bag.
4. the colloid gold test paper of a kind of fast detecting metalaxyl residue according to claim 1 is characterized in that gold mark pad is as colloid gold label by the metalaxyl monoclonal antibody.
5. according to the colloid gold test paper of the described a kind of fast detecting metalaxyl residue of claim 1, the garden stuff pesticide residue examination criteria metalaxyl content that the detected value that it is characterized in that test wire is set at country's promulgation is 0.5mg/kg.
6. according to the colloid gold test paper of the described a kind of fast detecting metalaxyl residue of claim 1, the granule fruit Detecting Pesticide standard metalaxyl content that the detected value that it is characterized in that test wire is set at country's promulgation is 1mg/kg.
7. according to the colloid gold test paper of the described a kind of fast detecting metalaxyl residue of claim 1, the raw grain Detecting Pesticide standard metalaxyl content that the detected value that it is characterized in that test wire is set at country's promulgation is 0.05mg/kg.
8. according to the colloid gold test paper of the described a kind of fast detecting metalaxyl residue of claim 1, it is 50ng/ml that the detected value that it is characterized in that test wire is set at limit of identification metalaxyl content.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
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| CN 200520106172 CN2833595Y (en) | 2005-08-24 | 2005-08-24 | Colloid gold test paper for quick detection of metalaxyl residue |
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| Application Number | Priority Date | Filing Date | Title |
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| CN 200520106172 CN2833595Y (en) | 2005-08-24 | 2005-08-24 | Colloid gold test paper for quick detection of metalaxyl residue |
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| CN2833595Y true CN2833595Y (en) | 2006-11-01 |
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| CN 200520106172 Expired - Fee Related CN2833595Y (en) | 2005-08-24 | 2005-08-24 | Colloid gold test paper for quick detection of metalaxyl residue |
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101949930A (en) * | 2010-09-13 | 2011-01-19 | 无锡市金坤生物工程有限公司 | Disc for fast detecting mycotoxin indexes |
| CN105158472A (en) * | 2015-08-26 | 2015-12-16 | 贵州勤邦食品安全科学技术有限公司 | Enzyme linked immunosorbent assay kit for detecting metalaxyl residues and using method |
| CN105259351A (en) * | 2015-08-26 | 2016-01-20 | 贵州勤邦食品安全科学技术有限公司 | Test paper for detecting metalaxyl residue and application thereof |
| CN105388286A (en) * | 2015-12-23 | 2016-03-09 | 中国烟草总公司郑州烟草研究院 | Test strip for detecting iprodione and preparation method and application of test strip |
| CN108802395A (en) * | 2018-06-20 | 2018-11-13 | 广州质量监督检测研究院 | Detect the detection card and its preparation method and application of Triclosan |
| CN109061150A (en) * | 2018-09-21 | 2018-12-21 | 中国烟草总公司郑州烟草研究院 | A kind of time-resolved fluoroimmunoassay chromatograph test strip and its preparation method and application detecting metalaxyl |
-
2005
- 2005-08-24 CN CN 200520106172 patent/CN2833595Y/en not_active Expired - Fee Related
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101949930A (en) * | 2010-09-13 | 2011-01-19 | 无锡市金坤生物工程有限公司 | Disc for fast detecting mycotoxin indexes |
| CN105158472A (en) * | 2015-08-26 | 2015-12-16 | 贵州勤邦食品安全科学技术有限公司 | Enzyme linked immunosorbent assay kit for detecting metalaxyl residues and using method |
| CN105259351A (en) * | 2015-08-26 | 2016-01-20 | 贵州勤邦食品安全科学技术有限公司 | Test paper for detecting metalaxyl residue and application thereof |
| CN105388286A (en) * | 2015-12-23 | 2016-03-09 | 中国烟草总公司郑州烟草研究院 | Test strip for detecting iprodione and preparation method and application of test strip |
| CN105388286B (en) * | 2015-12-23 | 2017-07-07 | 中国烟草总公司郑州烟草研究院 | A kind of test strips for detecting iprodione and its preparation method and application |
| CN108802395A (en) * | 2018-06-20 | 2018-11-13 | 广州质量监督检测研究院 | Detect the detection card and its preparation method and application of Triclosan |
| CN109061150A (en) * | 2018-09-21 | 2018-12-21 | 中国烟草总公司郑州烟草研究院 | A kind of time-resolved fluoroimmunoassay chromatograph test strip and its preparation method and application detecting metalaxyl |
| CN109061150B (en) * | 2018-09-21 | 2021-07-23 | 中国烟草总公司郑州烟草研究院 | Time-resolved fluorescence immunochromatographic test strip for detecting metalaxyl and preparation method and application thereof |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| EE01 | Entry into force of recordation of patent licensing contract |
Assignee: Beijing Wanhua Bioengineering Co., Ltd. Assignor: Wan Jicheng Contract fulfillment period: 2009.10.22 to 2014.10.22 Contract record no.: 2009110000260 Denomination of utility model: Colloid gold test paper for quick detection of metalaxyl residue Granted publication date: 20061101 License type: Exclusive license Record date: 20091030 |
|
| LIC | Patent licence contract for exploitation submitted for record |
Free format text: EXCLUSIVE LICENSE; TIME LIMIT OF IMPLEMENTING CONTACT: 2009.10.22 TO 2014.10.22; CHANGE OF CONTRACT Name of requester: BEIJING WANHUA BIOLOGY ENGINEERING CO., LTD. Effective date: 20091030 |
|
| C17 | Cessation of patent right | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20061101 Termination date: 20100824 |