CN2801788Y

CN2801788Y - Core rolling type rotary bottle device used for culturing anchorage-dependent cell

Info

Publication number: CN2801788Y
Application number: CN 200520083237
Authority: CN
Inventors: 韩毅鹏; 张潇文
Original assignee: Individual
Current assignee: Individual
Priority date: 2005-05-18
Filing date: 2005-05-18
Publication date: 2006-08-02
Anticipated expiration: 2015-05-18

Abstract

The utility model relates to a novel rotary bottle culture apparatus used for raising the adherent culture productivity of an animal cell. A rolling core of which the wall is provided with holes and the interior is provided with a solid phase carrier is added in the culture rotary bottle, wherein the solid phase carrier in the rolling core is composed of glass, ceramic and cellulose or composite materials. When the rolling core is put in the rotary bottle to make the cell culture, along with the rotation of the rotary bottle, the rolling core also rotate in the bottle. The cultured cells not only can grow on the bottle wall of the rotary bottle but also can grow by means of the surface of the carrier in the rolling core, therefore the bigger adherent area can be provided to culturing cell of the traditional rotary bottle.

Description

A kind of novel core formula rolling bottle device that rolls that is used for the attached cell cultivation

Technical field:

The utility model relates to a kind of novel rolling bottle culture apparatus that is used to improve zooblast adherent culture productive rate.

Background technology:

Rolling bottle (roller bottle) culture technique (also claim " rolling a bottle technology ") is meant and adopts the cylindrical culturing bottle system of rolling to cultivate anchorage dependent animal cell.Rolling bottle is cultivated and can be utilized almost whole inner wall surface of bottle to carry out cell cultures.Although the time in office only has the internal surface area of 15-20% to be covered by nutrient solution, the rotation of bottle makes cell alternately contact with nutrient solution, air.This mode has reduced the volume of required substratum, and bigger headspace is kept enough oxygen needs in the utilization bottle.Because the rolling bottle culture technique is a kind of many procedures systems, therefore exist labour intensity big, unit volume provides the surface-area of cell growth little, and it is low by volume to calculate cell yield, manpower and material service efficiency are not high, monitor and the condition of controling environment such as is restricted at shortcoming.But, since its simple in structure, less investment, technology maturation, and favorable reproducibility amplify characteristics such as just increasing rolling bottle quantity simply, so the rolling bottle system is still the technological method that production firm still widely adopts at present in scale production.For fully improving the production efficiency of rolling bottle system, some researchs had all once been carried out at the internal surface area that increases rolling bottle both at home and abroad.The United States Patent (USP) of having reported at present has US4962033, US5101013, US5527705; Chinese patent has CN2106834u and CN2040101u.The technical scheme of having developed mainly contains following several: (1) spiral rolling bottle (SpiraCel); The Spiracel rolling bottle that Bibby Sterilin company uses is a kind of helix poly vinylbenzene rolling bottle, and the even substep of cell in spiral is extremely important during use, otherwise can cause growth very uneven, and output also can be very low.Owing to can only see the cell of spiral outside during microscopy, might create a false impression if cell distribution is inhomogeneous.(2) Glass tubing Bellco-Corbeil culture systems; This is to be filled with by the isolating parallel cuvette of siloxanes stripper loop to form in rolling bottle.By liquid storage bottle perfusion culture liquid in the culturing bottle of forward and reverse 360 degree rotations that hocket.(3) the ImmobaSil rolling bottle of the ESRB rolling bottle of BibbySterilin (Corning) company or Ashby Scientific and Integra Biosciences.This is to have adopted the surperficial rolling bottle that fluctuating is arranged, in the hope of can increase the surface-area of cultivating under same diameter.Above-mentioned these products must adopt special manufacture craft production, cost of manufacture height not only, and if need in producing to reuse, then must carry out loaded down with trivial details cleaning and disinfecting steps.In addition, for the manufacturer that adopts traditional rolling bottle (roller bottle), if be replaced with above-mentioned these rolling bottles, just mean that original a large amount of bottle will all eliminate, this is undoubtedly huge waste.

The technical scheme that the utility model adopted is in order effectively to increase the adherent surface-area of rolling bottle (roller bottle), in existing culturing bottle, increase porose on the wall, in the core that rolls of solid phase carrier is housed.The carrier that rolls in-core is made of glass microsphere or synthetic materials, is placed on when carrying out cell cultures in the rolling bottle when rolling core, along with the rotation of rolling bottle, rolls core and also rolls in bottle.Not only can on the bottle wall of rolling bottle, be grown by cultured cells, also can grow simultaneously by means of the carrier surface that rolls in-core portion.Rolling bottle with roll core and constituted the novel core formula of the rolling rolling bottle culture apparatus of a cover.The utility model can be under the condition that does not change original basic culture device and technology, for cell provides bigger adherent area.Because the core that rolls of the utility model rolling bottle device is made simply, uses easily, and is with low cost, can reuse.After adopting this device to improve, not only can not cause a large amount of discarded of original culturing bottle, original production technique is not needed to do big adjustment yet, just can effectively increase the cultivation productive rate of attached cell.

Description of drawings:

The utility model is described in further detail below in conjunction with drawings and Examples.

Fig. 1 is the principle schematic of novel rolling bottle device

Fig. 2 is the structural representation that rolls core

Implementation step:

In Fig. 1, the utility model rolling bottle device mainly is by traditional cultivation rolling bottle (roller bottle) (1) and rolls core (2) formation.

In embodiment illustrated in fig. 2, roll core (2) and form by termination sieve cap (3), body (4) and solid phase carrier (5).On the body that rolls core (2) (4) outer wall, be covered with the irregular aperture of 0.1-5 millimeter, the maximum outside diameter that rolls termination sieve cap (3) of core two ends d should be greater than the external diameter of body (4), and making to roll only has end regions contact rolling bottle (1) inwall when core (2) rolls in rolling bottle (1).The middle part meshed portion of body can not contact a bottle wall.Roll the length of the total length outline of core (2) less than the vertical wall section of rolling bottle (1), the diameter of termination, two ends largest portion should be less than rolling bottle (1) bottleneck diameter, makes to roll core (2) and can be smoothly put into rolling bottle (1) or take out from bottleneck.During use, pack into greater than the solid phase carrier of cinclides in rolling core (2), the material of solid phase carrier is (but be not limited to be) glass, pottery, Mierocrystalline cellulose, chitosan, dextran, gelatin or various macromolecule polymeric material.After pre-treatment and sterilization, will roll core and put into rolling bottle from the rolling bottle bottleneck, when rolling bottle rolled, rolling core also can be along with rolling together in bottle.Add nutrient solution and cell kind, put and carry out the rolling bottle cultivation on the Rotary Machine, can be utilized the inwall of rolling bottle and the solid phase carrier that rolls in-core to carry out adherent growth by cultured cells simultaneously.

Claims

1, a kind of novel core formula rolling bottle culture apparatus that rolls that is used to improve zooblast adherent culture productive rate, it is characterized in that: be made of the rolling bottle (1) and the core (2) that rolls that solid phase carrier is housed, the surface of solid phase carriers and the rolling bottle inwall that roll in the core can provide adherent surface-area for cell cultures simultaneously.

2, a kind of device according to claim 1 is characterized in that: roll core (2) and be made up of termination sieve cap (3), body (4), solid phase carrier (5).

3, a kind of device according to claim 1, it is characterized in that: the aperture that on the body that rolls core (2) (4) outer wall, is covered with the 0.1-5 mm dia, roll the external diameter of termination sieve cap (3) maximum outside diameter at core two ends greater than body (4), the total length that rolls core (2) is long less than the straight wall wall of rolling bottle (1), and the diameter of termination, two ends largest portion is less than rolling bottle (1) bottleneck diameter.

4, a kind of device according to claim 1 is characterized in that: the solid support material of packing in rolling core (2) is granular glass or other macromolecule polymeric material of diameter greater than body (4) cinclides.