CN2636229Y - Magnetic field device for molecular lighting analysis - Google Patents
Magnetic field device for molecular lighting analysis Download PDFInfo
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- CN2636229Y CN2636229Y CN 03272395 CN03272395U CN2636229Y CN 2636229 Y CN2636229 Y CN 2636229Y CN 03272395 CN03272395 CN 03272395 CN 03272395 U CN03272395 U CN 03272395U CN 2636229 Y CN2636229 Y CN 2636229Y
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Abstract
The utility model relates to a magnetic field device, in particular to a magnetic device for molecular luminescence analysis. The utility model is provided with a base, a sample cell holder, a sample cell room, a magnet, a magnet bracket, a guide rail and a guide rail bracket. Wherein, the sample cell holder is provided on the base and the sample cell room is arranged over the sample cell holder; an incident light port and an emergent light port are provided in the sample cell room; a couple of magnets are arranged on the magnet bracket; the sample cell room is positioned between the magnets; the magnet bracket is positioned on the guide rail which is arranged on the guide rail bracket and the guide rail bracket is positioned on the base. The utility mode realizes the simultaneous and rapid detection of the multicomponent polycyclic aromatic hydrocarbon and is not only beneficial for the forming of the phosphor but also can restrain the effect of the quenching factor on the room temperature phosphorescence to a certain extent when studying the effect of the magnetic field effect in the ordered medium system on the room temperature spectrum. Besides, the utility model provides a very effective research method for the influence of the field detection magnetic field effect on the in vivo molecular and revealing the essence of the biomagnetic effect especially when applied in the research fields such as biological system and life science.
Description
(1) technical field
The utility model relates to a kind of magnetic field device, especially a kind of magnetic field device that is mainly used in the mulecular luminescence analysis.
(2) background technology
Because the mulecular luminescence analytic approach has highly sensitive advantage, the application in life science, environmental science, commercial production more and more widely.In the mulecular luminescence analysis, often run into the interference of the scattered light of solvent, analysis is had remarkable influence, become the key constraints of mulecular luminescence sensitivity of analytical method.Synchronous fluorimetry is because adopted the synchronous scanning technology to make the selectivity of method be able to further raising, but the interference of solvent scattered light is still the big obstacle that detection sensitivity further improves.Polarization, low temperature and ultrasonic jet flow technology can help to suppress scattered light, but polarization technology can not be eliminated the interference of scattered light fluctuation; Though cryogenic technique and ultrasonic jet flow technology make the selectivity of method that improvement be arranged greatly, instrument and operate all more complicated.
(3) summary of the invention
The purpose of this utility model is to provide a kind of externally-applied magnetic field effect (External magnetic field Effects, be called for short MFE) is introduced the mulecular luminescence analysis field, to improve sensitivity that original mulecular luminescence analyzes and magnetic field device optionally.
For the convenience that does not change the original accessory of instrument structure and use, the utility model is according to the design feature of fluorospectrophotometer sample camera bellows, designed to be convenient to permanent magnetic field effect is imposed on magnetic field device on the on-the-spot sample.
The utility model is provided with base, sample cell frame, sample cell chamber, magnet, magnet carrier, guide rail and rail brackets.Sample cell is set up on the base, is used to be provided with the sample cell chamber; Sample cell frame top is located in the sample cell chamber, is used to place sample cell and sample, offers incident light mouth and emergent light mouth in the sample cell chamber, preferably establishes aquaporin on the sample cell chamber, and the passway connects the recirculated water conduit, is used to control the sample cell experimental temperature; The magnet that pair of magnet is formed is located on the magnet carrier, and the sample cell chamber is between magnet; Magnet carrier is located on the guide rail, can move along guide rail, and guide rail is located on the rail brackets.Front end at guide rail is preferably established block, is used to limit the shift position of magnet.
During use, at first the utility model is put into the camera bellows of fluorospectrophotometer, put into the sample chamber after again the sample of required mensuration being changed over to sample cell, measure; If desired sample is magnetized, magnet is pushed into the block front end by guide rail, can measure.
Can suppress effectively the to resonate fluctuation of solvent scattered light in the synchronous scanning process of the externally-applied magnetic field effect of utilizing this device to provide, magnetic effect-resonance synchronous fluorimetry, magnetic effect-polarization-resonance synchronous fluorimetry have been set up according to this, and when measuring when said method is used for the polycomponent palycyclic aromatic, the sensitivity of method and selectivity are greatly improved.And these methods are simple, make originally the job simplification that can only just can finish by complex instrument.Palycyclic aromatic is a ubiquitous organic contaminant in the environment, because of it has carcinogenic, teratogenesis, mutagenic " three cause " effect gets more and more people's extensive concerning.Fast detecting when the externally-applied magnetic field effect of utilizing this device to provide has realized many components palycyclic aromatic has positive meaning.
When utilizing magnetic field effect that this device provides magnetic field effect be to the influencing of room temperature phosphorimetry spectrum in research ordered media system, find that magnetic field effect not only helps the formation of phosphor, and can suppress of the influence of quencher factor to a certain extent its room temperature phosphorimetry.When particularly this device is used for research field such as living things system, life science, detects magnetic field effect for the scene and provide a kind of very effective research means living body molecule influence, the essence that discloses biomagnetic.
This device has following characteristics:
A. former instrument is not done any change, in can on-the-spotly magnetic effect be introduced the mulecular luminescence analysis and detect;
B. take very conveniently, and cost is very cheap, so this device has certain value of popularizing;
C. realized the execute-in-place of magnetic field effect, this research to biomagnetism has great significance;
D. possess circulating water device, can be used for controlling the temperature of experiment.
(4) description of drawings
Fig. 1 is the structural drawing of the utility model embodiment.
Fig. 2 is the front view of Fig. 1.
Fig. 3 is the rear view of Fig. 1.
Fig. 4 is a pyrene (10 in the cyclohexane solution
-6Mol/L) emission spectrum.Wherein spectral line a is for using former instrument annex gained, and spectral line b is for using the utility model gained.
Fig. 5 is the spectrum of ethanol.Wherein spectral line a is the resonance synchronous fluorescent spectrum, and spectral line b is magnetic effect-resonance synchronous fluorescent spectrum.
Fig. 6 is the spectrum of En, perylene mixed liquor.Wherein spectral line a is the resonance synchronous fluorescent spectrum, and spectral line b is magnetic effect-resonance synchronous fluorescent spectrum.
Fig. 7 is fluorenes, acenaphthene, anthracene, the benzo [spectrum of a] Bi, perylene mixed liquor.Wherein spectral line a is the resonance synchronous fluorescent spectrum, and spectral line b is magnetic effect-polarization-resonance synchronous fluorescent spectrum.
(5) embodiment
Following examples will be formed structure of the present utility model in conjunction with the accompanying drawings and give prominence to effect and do detailed explanation.
Shown in Fig. 1～3, the utility model is provided with base 1, sample cell frame 2, sample cell chamber 3, magnet 4, magnet carrier 5, guide rail 6 and rail brackets 9.Base is separately fixed on 3 the outstanding screws in instrument camera bellows bottom, can take off from former instrument easily.The sample cell frame is made with the plastics of black, suitable sample cell is applied magnetic field and is fixed on the base.Sample cell frame top is located in the sample cell chamber, is used to place sample cell and sample, offers incident light mouth 32 and emergent light mouth 31 in sample cell chamber 3, makes the centre of its center in light path.On the sample chamber, add the conduit 7 that can lead to recirculated water, for use in the temperature of control experiment.Pair of magnet 4 is located on the magnet carrier 5, and sample cell chamber 3 is between magnet 4; Magnet carrier 5 is located on the guide rail 6, can move along guide rail, and guide rail is located on the rail brackets 9, so that the sample cell chamber is in the central authorities of two block permanent magnets.Front end at guide rail is preferably established block 8, is used to limit the shift position of magnet.Said guide rail can adopt two dovetail groove guide rails, is convenient to the slip of magnet.It is that 270mT, specification are that the permanent magnet of 1cm * 2cm * 4cm (high * wide * long) is formed that used magnet adopts 2 field intensity, and two blocks of magnet does not have magnet carrier 5 that the carbon-free iron that prolongs magnetic makes by one to be supported and link and be linked to be the loop.So not only can increase the intensity in its magnetic field that forms, can play certain magnetic screening action simultaneously, and reduce of the influence of used magnetic field as far as possible surrounding environment.Magnetic field intensity can be regulated by regulating two distances between the magnet.Following magnet posts a slice slide plate, and whole magnet can slidably reciprocate on guide rail by it.
The position of magnet when the block 8 of the front end setting of guide rail is used for fixing experiment, with guarantee added magnetic field intensity good reappearance arranged.
In order to investigate performance of the present utility model, under identical instrument condition, be under the condition of 320nm, to have measured 10 in excitation wavelength with former instrument annex and the utility model respectively
-6The fluorescence spectrum of the cyclohexane solution of mol/L pyrene is referring to Fig. 4 (horizontal ordinate is a wavelength X, and unit is nm, and ordinate is relative intensity I).
As seen from Figure 4, the spectral line characteristic basically identical of collection of illustrative plates a and b, just the relative intensity of collection of illustrative plates b is a little less than collection of illustrative plates a.By the utility model gained fluorescence intensity a little less than the fluorescence intensity that is obtained with former instrument annex, because luminous flux of the present utility model causes less than former instrument annex.Because fluorescence intensity itself is exactly a relative value, the problem of luminous flux size can be regulated by the sensitivity of instrument detecting device.So the utility model is the fluorescence spectral characteristic of image study object fully really.
Referring to Fig. 5, the externally-applied magnetic field effect that provides can suppress organic solvent scattered light and fluctuation thereof effectively, and when not having the action of a magnetic field as seen from Figure 5, the fluctuation of the resonance synchronous fluorescent spectrum of ethanol is very strong; And after under the action of a magnetic field ethanol being carried out the magnetization of certain hour, fluctuation of its resonance synchronous fluorescent spectrum is suppressed greatly.
Investigated the inhibiting effect of magnetic field effect to common twenties kinds of organic solvent scattered lights and fluctuation.According to organic solvent scattered light and fluctuation with and by the situation that magnetic field effect suppresses, above-mentioned twenties kinds of organic solvents are divided three classes: scattered light and fluctuation are big, are suppressed effect remarkable (98% of its scattered light and fluctuation is suppressed during as ethanol medium); Scattered light and fluctuation are general, and it is general to be suppressed effect; Scattered light and fluctuate for a short time, it is not remarkable to be suppressed effect.
Magnetic effect-resonance synchronous fluorimetry, magnetic effect-polarization-resonance synchronous fluorimetry have been set up with the externally-applied magnetic field effect that the utility model provides.When synchronous fluorimetry detects multiple measured object at the same time, be the selectivity that obtains, often select less Δ λ value, at this moment, the serious interference of solvent scattered light.Therefore the resonance synchronous fluorimetry is difficult to realize.And the interference of magnetic field effect when can be used for suppressing the fluctuation of solvent scattered light the resonance synchronous fluorescence being analyzed.Set up magnetic field effect-resonance synchronous fluorimetry in view of the above.
With En, perylene mixed solution is the model thing, has investigated the analytical characteristics of magnetic effect-resonance synchronous fluorimetry.The result shows that magnetic effect-resonance synchronous fluorimetry makes the fluorescence peak of En, perylene be able to good separation (referring to Fig. 6).And when not having the action of a magnetic field, the resonance synchronous fluorescence signal of En, perylene is almost covered by solvent scattered light noise, and its fluorescence intensity can't accurately be measured.Further experimental result shows, when magnetic effect-resonance synchronous fluorimetry was measured En, perylene simultaneously, the relative standard deviation that the relative standard deviation of mensuration anthracene is 2.3%, Ce is Dinged perylene was 5.2%; The detectability of En, perylene is respectively 0.14ng/mL and 0.027ng/mL, and the more original method of sensitivity is improved tens times.
Magnetic effect can suppress the interference of solvent scattered light fluctuation, polarization technology can suppress the interference of scattered light from the scattered light aggregate level, set up magnetic effect-polarization-resonance synchronous fluorimetry again with the externally-applied magnetic field effect that this device provides, obtained better analysis result.
With , En, perylene mixed solution is the model thing, has investigated the analytical characteristics of magnetic effect-polarization-resonance synchronous fluorimetry.The result shows when magnetic effect-polarization-resonance synchronous fluorimetry is measured , En, perylene simultaneously, and the relative standard deviation that the relative standard deviation of measuring anthracene is 2.0%, Ce is Dinged perylene is 2.7%; The detectability of En, perylene is respectively 0.074ng/mL and 0.0039ng/mL, and its analytical performance has further improvement than magnetic effect-resonance synchronous fluorimetry.Simultaneously, this method is successfully used to fluorenes, acenaphthene, anthracene, [a] Bi, perylene five components are fast detecting simultaneously, has obtained satisfied result (referring to Fig. 7) for benzo.
Above-mentioned experimental result shows that the externally-applied magnetic field effect that the utility model provides can be used for improving sensitivity and the selectivity that mulecular luminescence is analyzed.
Carry out the research of relevant mulecular luminescence mechanism with the utility model.Experimental result shows magnetic field effect, and (β-CD)/(α-BrNp) fluorescence spectrum and room temperature phosphorimetry (RTP) spectrum of system are all influential for alpha-bromonaphthalene to beta-schardinger dextrin-.For this reason, studied the mechanism of action between β-CD, α-BrNp, solubility fatty alcohol and magnetic field effect the mechanism of action with fluorescence method, fluorescent polarized light spectrometry, absorption spectroscopy and RTP method to its influence.Proposed to form 2:2 between β-CD, α-BrNp, solubility fatty alcohol: the neodoxy of 2 overlapping inclusion complexs and magnetic field effect help suppressing the cancellation effect of dissolved oxygen DO, the neodoxy of increase S1 → T1 transition probability.
According to the interaction between β-CD, α-BrNp, solubility fatty alcohol is this experimental phenomena of long response time, can set up dynamics RTP method, and uses it for analyzing organic substance and investigate the influence of magnetic field effect to them as a research means.The result shows that magnetic field effect can improve the analytical performance of dynamics RTP method.
Above-mentioned new, interesting experimental phenomena not only provides new evidence for magnetic field effect can be used for the mulecular luminescence analysis field, and the research that forms mechanism for some supramolecular systems simultaneously provides new means, method.
In addition, magnetic field effect (or electromagnetic radiation) also is the problem that we are concerned about from the beginning to the influence of biosome and relevant therewith biomagnetism, the relevant issues of magnetobiology, and fluorescence polarization method is the strong means of these relevant issues of research.We have investigated the influence of magnetic field effect to the fluorescence polarization spectrum of glycerine medium Zhong perylene with fluorescence polarization method for this reason.The result shows that the degree of polarization of perylene Xia used magnetic field intensity increases 13%.
The acquisition of The above results not only has important significance for theories, has more realistic meaning simultaneously.Magnetic field effect not only provides new means for studying relevant photochemical reaction mechanism to some photoinduced electronics, influential this result of charge transfer process, also provides new thinking for research, more synthetic photochromicss, components and parts.Magnetic field effect is to influential this experimental result of fluorescence polarization spectrum of glycerine medium Zhong perylene, is research magnetic therapy medicine, the device mechanism of action in diseases prevention, in curing the disease, for the work place study magnetic field effect to the influence of living body molecule provide may with new research means.
Claims (5)
1, is used for the magnetic field device that mulecular luminescence is analyzed, it is characterized in that being provided with base, sample cell frame, sample cell chamber, magnet, magnet carrier, guide rail and rail brackets; Sample cell is set up on the base, and sample cell frame top is located in the sample cell chamber, offers incident light mouth and emergent light mouth in the sample cell chamber, and pair of magnet is located on the magnet carrier, and the sample cell chamber is between magnet; Magnet carrier is located on the guide rail, and guide rail is located on the rail brackets, and rail brackets is located on the base.
2, the magnetic field device that is used for the mulecular luminescence analysis as claimed in claim 1 is characterized in that establishing block at the front end of guide rail.
3, the magnetic field device that is used for the mulecular luminescence analysis as claimed in claim 1 is characterized in that establishing aquaporin on the sample cell chamber, and the passway connects the recirculated water conduit.
4, the magnetic field device that is used for the mulecular luminescence analysis as claimed in claim 1 is characterized in that said guide rail adopts two dovetail groove guide rails.
5, as claimed in claim 1ly be used for the magnetic field device that mulecular luminescence is analyzed, it is characterized in that said magnet adopts 2 block permanent magnets to form, two blocks of magnet does not have magnet carrier that the carbon-free iron that prolongs magnetic makes by one to be supported and links and be linked to be the loop.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 03272395 CN2636229Y (en) | 2003-07-10 | 2003-07-10 | Magnetic field device for molecular lighting analysis |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 03272395 CN2636229Y (en) | 2003-07-10 | 2003-07-10 | Magnetic field device for molecular lighting analysis |
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| Publication Number | Publication Date |
|---|---|
| CN2636229Y true CN2636229Y (en) | 2004-08-25 |
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ID=34301068
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 03272395 Expired - Fee Related CN2636229Y (en) | 2003-07-10 | 2003-07-10 | Magnetic field device for molecular lighting analysis |
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| Country | Link |
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| CN (1) | CN2636229Y (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104568863A (en) * | 2013-10-28 | 2015-04-29 | 南开大学 | Magnetic-particle fluorescence aggregation detecting device |
| CN110153121A (en) * | 2019-06-21 | 2019-08-23 | 苏州长光华医生物医学工程有限公司 | It is a kind of for cleaning the magnetic particle adsorbent equipment and its cleaning equipment of reaction cup |
| CN110907411A (en) * | 2019-11-20 | 2020-03-24 | 华南理工大学 | Steady-state luminous magnetic field effect tester |
-
2003
- 2003-07-10 CN CN 03272395 patent/CN2636229Y/en not_active Expired - Fee Related
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104568863A (en) * | 2013-10-28 | 2015-04-29 | 南开大学 | Magnetic-particle fluorescence aggregation detecting device |
| CN110153121A (en) * | 2019-06-21 | 2019-08-23 | 苏州长光华医生物医学工程有限公司 | It is a kind of for cleaning the magnetic particle adsorbent equipment and its cleaning equipment of reaction cup |
| CN110907411A (en) * | 2019-11-20 | 2020-03-24 | 华南理工大学 | Steady-state luminous magnetic field effect tester |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C19 | Lapse of patent right due to non-payment of the annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |