CN2625889Y - A quantitative determination instrument for chroma of immunity detecting test strip - Google Patents
A quantitative determination instrument for chroma of immunity detecting test strip Download PDFInfo
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- CN2625889Y CN2625889Y CN 03249212 CN03249212U CN2625889Y CN 2625889 Y CN2625889 Y CN 2625889Y CN 03249212 CN03249212 CN 03249212 CN 03249212 U CN03249212 U CN 03249212U CN 2625889 Y CN2625889 Y CN 2625889Y
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Abstract
The utility model relates to a quantitive chroma detector for immunity detection litmus that pertains to medical equipments field, consisting of a photodetector, a fixing slot of a litmus strip, a housing, a circuit for handling photoelectrocity signals and displaying results; the photodetector comprises a lighting device and two photosensitive devices which are symmetrically arrayed at both sides thereof, and the lighting device and photosensitive devices aims at the fixing slot of the litmus strip, the circuit of the photodetector comprises a signal magnifying circuit, a signal integration circuit, a modulus transition circuit and a displaying device. The utility model has the advantages of direct and precise detecting results, compact machine structure and simple operating, which needs not to debug when in use.
Description
Technical field: the utility model relates to immunity test strip bar colourity detection by quantitative instrument, belongs to medical instruments field.
Background technology: utilize the various quick diagnosis of immunochromatography single stage method principle and labelled antibody or antigen preparation or detect test paper,, in clinical examination, obtained to use comparatively widely as gold mark detection test paper.The detection test paper that is used for clinical examination at present on the international market has over one hundred kind, comprises many aspects such as early pregnancy, ovulation, venereal disease, early-stage cancer, various hepatitis, acquired immune deficiency syndrome (AIDS) and drugs.Adopt the mark test paper to do clinical examination, have directly perceived, quick, easy and simple to handle, economic dispatch characteristics, many products also can be directly used in family or individual people detection, as very early pregnancy and ovulation tests.These characteristics of immunity chromatography detection test paper have greatly made things convenient for the user, also make quick detection test paper become industry with fastest developing speed in the biomedicine.But such test paper can only qualitatively judge by means of having or not color or shade to change basically at present, and artificial subjective factor is big, owing to can not carry out quantitative measurement the range of application of test paper is very limited simultaneously.With ovulation test paper (interstitialcellstimulating hormone (ICSH) (LH) detects test paper) is example, qualitative detection can only detect one roughly the onset of ovulation and whether ovulation is arranged, in fact the quantitative measurement of LH all has very important use in hormone secretion detection, medical diagnosis on disease and treatment, also must adopt in these quantitative or semiquantitative detections at present as enzyme and exempt from, puts and exempt from or method such as photochemistry is carried out.These methods not only cost an arm and a leg, and it is loaded down with trivial details to detect step, also needs the instrument of professional and particular arrangement just can carry out, and have therefore seriously limited carrying out and using of these test items, have hindered the raising of medical detection level.
The interpretation as a result that detects at present test paper is by naked eyes basically, has or not colour band to occur after detection or the depth that colour band occurs is come judged result according to test strips.This determination methods can only reach the requirement of carrying out qualitative determination, and getting sth into one's head property is strong, therefore may be just inapplicable to diagnosing significant project to only reaching quantitative measurement.
From the document Investigation result, though existing scholar and R﹠D institution have noticed the importance of mark test paper detection by quantitative problem, and have carried out some preliminary discussions, still there is not ripe scheme, more there is not ready-made product.The quantitative interpretation of mark test paper belongs to colourity discrimination technology field, and this respect technology that exists has colorimeter and bar code reading machine at present, but these two technology all can not be directly used in the detection by quantitative of immune chromatography test paper.The bar code reading machine can only be judged having or not of striped, can not discern the depth of striped, therefore can not be used for quantitatively.Colorimeter can be discerned the depth of striped, but is difficult to adapt to the test condition of immunochromatography mark test paper.There is the paper substrate color distortion in the mill in the mark test paper, mostly is in moistening and variable condition in using the mensuration state, makes the adjustment of colorimeter and calibration very difficult like this, and it is accurate therefore to be difficult to quantitative measurement, to reach the accuracy requirement of mensuration.Existing in addition colorimeter is complex structure mostly, operates loaded down with trivial detailsly, and the layman is difficult to finish, and therefore is difficult to promote.The instrument that also has some to cooperate test strip to use on the market occurs, and as blood glucose meter and ten detectors of urine, these instruments all can only carry out tentatively qualitative determination, can not carry out accurate reading.From document Investigation, there are many researchers to have been noted that the importance of mark quick detection test paper quantitative measurement as a result, but substantially propose to solve the method or the instrument of quantitative measurement or solution quantitative interpretation.
Summary of the invention: the purpose of this utility model is to overcome above-mentioned prior art defective, invent the two probe of a kind of employing automatic tracking and compensating mode, make the detector of designed manufacturing can follow the wetness conditions of vitta paper substrate and paper slip automatically, interpretation goes out the aberration of vitta, realize the detection by quantitative of mark paper slip, quantitatively or sxemiquantitative reading machine or detector, solve qualitative, the quantitative measurement problem of marker detection test strips, its design concept science, easy and simple to handle, measure accurate, cheap a kind of qualitatively immunity test strip bar of test paper colourity detection by quantitative instrument.
The technical solution of the utility model: this immunity test strip bar colourity detection by quantitative instrument by photodetection head (2), test strips locating slot (3), casing (1), Photoelectric Signal Processing and as a result display circuit form.Photodetection head, Photoelectric Signal Processing and as a result display circuit evenly place in the casing, the test strips locating slot is fixed on casing outer surface, corresponding casing place offers and checks aperture (4) with it.The photodetection head by a luminescent device and two respectively the symmetric offset spread photosensitive device that is 2045 ° of angles (β) in its both sides and with it form, luminescent device and photosensitive device are all aimed at the detection aperture (4) on the test strips locating slot place casing, the light that luminescent device is sent is radiated on the detection test paper by detecting aperture, and the reflected light that detects test paper simultaneously can be absorbed by photosensitive device; Luminescent device can be ordinary monochromatic light source light-emitting components such as light emitting diode or laser diode, and its effect is the paper slip that produces the rayed mark of special frequency spectrum; Photosensitive device can be normal optical photosensitive elements such as photodiode, photoresistance, and its effect is the reflected light that acceptance reflects through mark paper slip detection line, and light signal is converted into electric signal.The test strips locating slot is the sulculus that plastic or other material is made, and fixing (methods such as available glue or screw) have the position of checking aperture at casing outer surface, for inserting and fixing tested paper slip.
The peak wavelength of the spectrum of the utility model luminescent device and test strips colour band color be suitable, two optical receiver receiving spectrum wavelength and colour band spectral wavelength are suitable.Photoelectric Signal Processing and as a result display circuit comprise signal amplification circuit, signal integration circuit, analog to digital conversion circuit, display circuit and power circuit; Signal amplification circuit has two operational amplifiers (common operational amplifier) and respective peripheral electronic component to form, signal integration circuit, analog to digital conversion circuit, display circuit also are made up of operational amplifier (common operational amplifier) and respective peripheral electronic component, power circuit is made up of common power conversion integrated package and respective peripheral electronic component, and display device can be common light emitting diode, liquid crystal digital or pointer instrument.Two photosensitive devices are gone into end with two operational amplifiers of signal amplification circuit respectively and are linked to each other, the signal amplification circuit output terminal is gone into end through resistance and signal integration circuit computing amplifier and is linked to each other, and the signal integration circuit output end is gone into end through resistance with analog to digital conversion circuit or display circuit and linked to each other.
The various marker detection test paper that utilize immunochromatography single stage method principle design to produce, be to be fixed in a certain zone of diffusion barrier behind special monoclonal antibody or the label incorporation of markings such as antigen and collaurum earlier, when the sample end immersion of test strips or after sample area splashes into sample liquid, because capillarity, sample liquid will drive labels such as collaurum and move forward, when moving to the p-wire zone, with the corresponding antigens or the antibody of labelled antibody or antigen generation specific bond another antibody in tested line zone is discerned in the sample, the specific bond of " sandwich style " takes place, so stop, labels such as collaurum will demonstrate the color of respective markers, in certain sample concentration scope, in the depth of shown color and the sample liquid the concentration of the antigen of measuring or antibody be directly proportional.Like this, can provide the content of tested antigen in the sample or antibody by the depth of quantitative measurement vitta colourity.
Reading the optical principle that vitta colourity taked is: when a monochromic beam shines an opaque body surface, during as the p-wire colour band zone of marker detection test paper, light can be absorbed and reflect by body surface.Catoptrical intensity I along a certain direction
R1With incident intensity I
0Between the pass be:
I
R1=α(1-a-γ)I
0
In the formula a be object to this monochromatic absorption coefficient, γ is a transmission coefficient, α is a undetermined constant.
When monochromatic light shone the color development area of test strip, the label particles that a part is accumulated on the vitta absorbed, absorbed photon number N
1iBe directly proportional with this regional label particles number N.
N
1i=βq(T)N
N is the label particles number in the unit volume.
By relatively impinging upon the reflected light I on the paper substrate
R1With the reflected light I that impinges upon on the vitta
R2Poor, just can measure the label particles number with antigen or antibodies indirectly, pass through logical calculated again, just can predict the concentration content of determined antigen in the sample or antibody.
The luminescent device emission light beam irradiates of the utility model by photodetection head (1) is on the paper slip to be tested, make reflected light I on the test strips paper substrate simultaneously
R1With the reflected light I on the vitta
R2Received by two photosensitive devices respectively, thereby obtain the colourity light signal of test paper to be measured, this signal through signal amplification circuit amplify with shaping after, to carry out logical process by the signal integration circuit, make signal correctly reflect the concentration of antigen in the sample or antibody, by analog to digital conversion circuit the photosignal of nonumericization is converted into numerical signal again, finally demonstrates test result (the concentration content of determined antigen or antibody in the sample) in modes such as sound, luminous or numerical value by display device.
The physical process of the whole mensuration of the utility model is as follows:
The logical process of light source → test strips → reflected light → reflected light reception → photoelectricity conversion → photosignal → result shows.
The utility model immunity chromatography detection test paper colourity detection by quantitative instrument is owing to adopt the automatic tracking and compensating technology, make the detector of designed manufacturing can follow the wetness conditions of vitta paper substrate and paper slip automatically, interpretation goes out the aberration of vitta, realizes the detection by quantitative of mark paper slip.It has measurement result directly, accurately, and compact integral structure is operated simple and easyly, need not carry out advantages such as any debugging during use, is a kind of novel suitable immunity test strip bar colourity detection by quantitative instrument.
Description of drawings: be described in detail below in conjunction with embodiment, but the utility model technology contents is not limited to accompanying drawing.
Fig. 1 is the synoptic diagram of the utility model immunity test strip bar colourity detection by quantitative instrument;
Fig. 2 is the utility model photodetection hair optical device and photosensitive device block position synoptic diagram; The centre position be luminescent device, the position, both sides be photosensitive device;
Fig. 3 is the utility model qualitative detection circuit diagram;
Fig. 4 is the utility model half-quantitative detection circuit diagram;
Embodiment: embodiment 1: as shown in Figure 1, 2, 3, this immunity test strip bar colourity detection by quantitative instrument (very early pregnancy-HCG detects test paper qualitative detection instrument) by photodetection 2, test strips locating slot 3, casing 1, Photoelectric Signal Processing and as a result display circuit form.Photodetection head, Photoelectric Signal Processing and as a result display circuit evenly place in the casing, the test strips locating slot is fixed on casing outer surface, corresponding with it casing place offers the inspection aperture.The photodetection head is by a light emitting diode D
1And two respectively symmetric offset spread in its both sides and be the photodiode D of 20 ° of angles with it
2, D
3Form, light emitting diode and photodiode are all aimed at the detection aperture (4) on the test strips locating slot place casing, and the test strips locating slot is the plastics sulculus, are fixed on casing outer surface with glue and have the position of checking aperture, for inserting and fixing tested paper slip.(λ=630nm) is suitable with test strips colour band color, the suitable (λ=630nm) of two optical receiver receiving spectrum wavelength and colour band spectral wavelength for the peak wavelength of the spectrum of luminescent device.
Photoelectric Signal Processing and as a result display circuit comprise signal amplification circuit, signal integration circuit, display circuit and power circuit.Signal amplification circuit is by two operational amplifier IC
1, IC
2(LM741) and corresponding resistor R
1~R
9Form signal integration circuit computing amplifier IC
3(LM741) and corresponding resistor R
11, R
12Form display circuit operational amplifier IC
4(LM741) and corresponding resistor R
13~R
15, redness and green LED D
4, D
5(display device) formed, and power circuit is by the power conversion ic chip
5(ME501) and respective peripheral component resistance, electric capacity, inductance, diode form.Photosensitive photodiode D
2, D
3Respectively with signal amplification circuit IC
1, IC
2Go into end and link to each other, signal amplification circuit goes out end through resistance R
11, R
12With signal integration circuit computing amplifier IC
3Go into end and link to each other, the signal integration circuit goes out end through resistance R
13With display circuit IC
4Link to each other.IC
1, IC
2And IC
3Be LM741.
When the luminous irradiation test strips of D1, the emission light of paper substrate and paper slip receives through D2, D3 respectively and becomes electric signal and enter operational amplifier IC
1With IC
2, two signals through amplifying are through resistance R
11-R
12Entering signal integrated circuit operational amplifier IC
3, at IC
3In compare, integrate back input display circuit operational amplifier IC
4, at IC
4In compare with preset reference value (negative, positive critical value of electrical signals), signal outputs to red light emitting diodes D during greater than reference value
4, red light is bright, and signal outputs to green LED D during less than reference value
4, green light is bright, thereby finishes qualitative test.
This detector shows test results by dichromatic LED, cooperates very early pregnancy (HCG) to detect test paper and uses, and concrete using method is:
1, gets very early pregnancy (HCG) and detect one on test paper, pick sample, then test strips is inserted the strip insertion groove of above-mentioned detector, fix by the operation requirement.
2, press power switch, energized, the instrument portable lamp is bright, instrument work, instrument self-clocking simultaneously.When timing during to 5 minutes schedule times, instrument display display result, red light is bright, and measurement result is positive, and green light is bright, and measurement result is negative.
Photoelectric Signal Processing and as a result display circuit comprise signal amplification circuit, signal integration circuit, analog to digital conversion circuit, display circuit and power circuit.Signal amplification circuit is by two operational amplifier IC
1, IC
2(LM741) and corresponding resistor R
1~R
10, capacitor C
1, C
2Form, the signal integration circuit is by operational amplifier IC
3(LM741) and corresponding resistor R
11~R
12, capacitor C
7Form, analog to digital conversion circuit is by ic chip
4(LM3914) and corresponding resistor R
13, R
14, R
15Form, display circuit is made up of 10 red light emitting diodes that link to each other with IC4 (LM3914) output terminal, and power circuit is by the power conversion ic chip
5(ME501), IC
6(ME7660) and respective peripheral component resistance, electric capacity, inductance, diode form.Photoresistance D
2, D
3Respectively with signal amplification circuit IC
1, IC
2Go into end and link to each other, signal amplification circuit goes out end through resistance R
9, R
10With signal integration circuit computing amplifier IC
3Go into end and link to each other, the signal integration circuit goes out end and analog to digital conversion circuit by ic chip
4Going into end links to each other.
When the luminous irradiation test strips of D1, the emission light of paper substrate and paper slip receives through D2, D3 respectively and becomes electric signal and enter operational amplifier IC
1With IC
2, two signals after the amplification are through resistance R
9, R
10Entering signal integrated circuit operational amplifier IC
3, at IC
3In compare, integrate back input analog to digital conversion circuit IC
4, compare with preset reference value (the electric signal sizes values corresponding), again from IC with variable concentrations
4Different port outputs to corresponding red light emitting diodes, indicates corresponding concentration value, thereby finishes the semidefinite property testing.IC
1, IC
2And IC
3Be LM741.
This detector cooperates lutropin-LH half-quantitative detection test paper to use, by the different numbers of elements that light emitting diode matrix is lighted, and the different magnitudes that show test results.10 red light emitting diodes represent respectively that LH concentration is 10,20,30,40,50,60,70,80,90, the different values of 100mIU/ml, and when the concentration of LH in the sample during in the respective concentration value, corresponding light emitting diode will be glittering.Concrete using method is:
1, gets one on lutropin (LH) half-quantitative detection test paper, pick sample, then test strips is inserted the strip insertion groove of above-mentioned detector, fix by the operation requirement.Press power switch, energized, the instrument portable lamp is bright, instrument work, instrument self-clocking simultaneously.When timing during to 15 minutes schedule times, the 3rd display lamp of instrument is glittering, and its corresponding reading in lower end is 30mIU/ml, shows that the concentration of LH should be 30mIU/ml ± 2.5mIU/ml in the sample.
2, get one on lutropin (LH) half-quantitative detection test paper, pick sample, then test strips is inserted the strip insertion groove of above-mentioned detector, fix by the operation requirement.Press power switch, energized, the instrument portable lamp is bright, instrument work, instrument self-clocking simultaneously.When timing during to 8 minutes schedule times, second and the 3rd display lamp of instrument is simultaneously glittering, second corresponding reading of display lamp is 20mIU/ml, and the 3rd the corresponding reading of display lamp is 30mIU/ml, shows that the concentration of LH should be 25mIU/ml ± 2.5mIU/ml in the sample.
Embodiment 3: shown in Fig. 1,2,4, immunity test strip bar colourity detection by quantitative instrument (lutropin-LH half-quantitative detection instrument) by photodetection 2, test strips locating slot 3, casing 1, Photoelectric Signal Processing and as a result display circuit form.Photodetection head, Photoelectric Signal Processing and as a result display circuit evenly place in the casing, the test strips locating slot is fixed on casing outer surface, corresponding with it casing place offers the inspection aperture.The photodetection head by a laser diode and two respectively symmetric offset spread in its both sides and the photodiode that is 30 ° of angles with it form, laser diode and photodiode are all aimed at the detection aperture on the test strips locating slot place casing; The test strips insertion groove is the sulculus that aluminium alloy is made, and is screwed at casing outer surface and has the position of checking aperture, for inserting and fixing tested paper slip.(λ=680nm) is suitable with test strips colour band color, the suitable (λ=680nm) of two optical receiver receiving spectrum wavelength and colour band spectral wavelength for the peak wavelength of the spectrum of luminescent device.
Photoelectric Signal Processing and as a result display circuit comprise signal amplification circuit, signal integration circuit, display circuit and power circuit.Signal amplification circuit is by two operational amplifier IC
1, IC
2(LM741) and corresponding resistor R
1~R
10, capacitor C
1, C
2Form, the signal integration circuit is by operational amplifier IC
3(LM741) and corresponding resistor R
11~R
13, capacitor C
7Form, display circuit is and IC
3The liquid crystal digital display that output terminal links to each other, power circuit is by the power conversion ic chip
5(ME501), IC
6(ME7660) and respective peripheral component resistance, electric capacity, inductance, diode form.Photoresistance D
2, D
3Respectively with signal amplification circuit IC
1, IC
2Go into end and link to each other, signal amplification circuit goes out end through resistance and signal integration circuit computing amplifier IC
3Going into end links to each other.
When the luminous irradiation test strips of D1, the emission light of paper substrate and paper slip receives through D2, D3 respectively and becomes electric signal and enter operational amplifier IC
1With IC
2, two signals after the amplification are through resistance R
9, R
10Entering signal integrated circuit operational amplifier IC
3, at IC
3In output to liquid crystal digital display after comparing, integrating, show corresponding concentration value, thereby finish qualitative test.
This detector cooperates the quantitative or half-quantitative detection test paper use of lutropin-LH, directly shows the result of mensuration by liquid crystal digital display.Concrete using method is:
1, gets one of lutropin-LH detection by quantitative test paper, pick sample, then test strips is inserted the strip insertion groove of above-mentioned detector, fix by the operation requirement.
2, press power switch, energized, the instrument portable lamp is bright, instrument work, instrument self-clocking simultaneously.When timing during to 10 minutes schedule times, instrument display shows reading 33.6, so the concentration of LH should be 33.6mIU/ml in the sample.
The utility model can be tested the various immunochromatography mark test paper of difformity and colourity by circuit conditioning preset signals reference value.By changing the accuracy class of circuit, cooperates different displays, can produce the series of tests instrument that is applicable to qualitative, sxemiquantitative, quantitative different accuracy grade.
The 1-casing; The 2-detecting head; 3-test paper locating slot; 4-checks aperture; β-luminescent device and two branches The 20-45 ° of angle that other symmetric arrays forms in its both sides and with it.
Claims (5)
1, a kind of immunity test strip bar colourity detection by quantitative instrument, it is characterized in that it by photodetection head (2), test paper locating slot (3), casing (1), Photoelectric Signal Processing and as a result display circuit form, photodetection head (2), Photoelectric Signal Processing and as a result display circuit all place in the casing, test strips locating slot (3) is fixed on casing (1) outside surface, and Dui Ying casing (1) locates to offer inspection aperture (4) with it.
2, a kind of immunity test strip bar colourity detection by quantitative instrument according to claim 1, it is characterized in that photodetection head (2) by a luminescent device and two respectively the symmetric offset spread photosensitive device that is 20-45 ° of angle (β) in its both sides and with it form, luminescent device and photosensitive device are all aimed at the detection aperture (4) on the test strips locating slot place casing.
3, a kind of immunity test strip bar colourity detection by quantitative instrument according to claim 1 and 2, it is characterized in that luminescent device spectrum peak wavelength and test strips colour band color is suitable, two optical receiver receiving spectrum wavelength and colour band spectral wavelength are suitable.
4, a kind of immunity test strip bar colourity detection by quantitative instrument according to claim 1 and 2, it is characterized in that Photoelectric Signal Processing and as a result display circuit comprise signal amplification circuit, the signal integration circuit, analog to digital conversion circuit, display circuit and power circuit, signal amplification circuit is made up of two operational amplifiers and respective peripheral electronic component, the signal integration circuit, analog to digital conversion circuit, display circuit also is made up of operational amplifier and respective peripheral electronic component, two photosensitive devices are gone into end with two operational amplifiers of signal amplification circuit respectively and are linked to each other, the signal amplification circuit output terminal is gone into end through resistance and signal integration circuit computing amplifier and is linked to each other, and the signal integration circuit output end is gone into end through resistance with analog to digital conversion circuit or display circuit and linked to each other.
5, a kind of immunity test strip bar colourity detection by quantitative instrument according to claim 4, it is characterized in that luminescent device spectrum peak wavelength and test strips colour band color is suitable, two optical receiver receiving spectrum wavelength and colour band spectral wavelength are suitable.
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CN 03249212 CN2625889Y (en) | 2003-06-23 | 2003-06-23 | A quantitative determination instrument for chroma of immunity detecting test strip |
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CN 03249212 CN2625889Y (en) | 2003-06-23 | 2003-06-23 | A quantitative determination instrument for chroma of immunity detecting test strip |
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Cited By (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN100342233C (en) * | 2003-06-23 | 2007-10-10 | 昆明云大生物技术有限公司 | Immunity detection and test paper scrip chroma quantitative determination instrument and detection method employed thereby |
WO2013041003A1 (en) * | 2011-09-21 | 2013-03-28 | 艾康生物技术(杭州)有限公司 | Biochemical analyzer and tester, and test method |
CN107561076A (en) * | 2017-07-28 | 2018-01-09 | 武汉市安友泽瑞科技有限公司 | The low cost optical electricity detecting system and method judged for the line color depth |
-
2003
- 2003-06-23 CN CN 03249212 patent/CN2625889Y/en not_active Expired - Lifetime
Cited By (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN100342233C (en) * | 2003-06-23 | 2007-10-10 | 昆明云大生物技术有限公司 | Immunity detection and test paper scrip chroma quantitative determination instrument and detection method employed thereby |
WO2013041003A1 (en) * | 2011-09-21 | 2013-03-28 | 艾康生物技术(杭州)有限公司 | Biochemical analyzer and tester, and test method |
CN107561076A (en) * | 2017-07-28 | 2018-01-09 | 武汉市安友泽瑞科技有限公司 | The low cost optical electricity detecting system and method judged for the line color depth |
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---|---|---|---|
C14 | Grant of patent or utility model | ||
GR01 | Patent grant | ||
AV01 | Patent right actively abandoned |
Effective date of abandoning: 20030623 |
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C25 | Abandonment of patent right or utility model to avoid double patenting |