CN2543066Y - Protein chip of covalent stationary biomolecule - Google Patents
Protein chip of covalent stationary biomolecule Download PDFInfo
- Publication number
- CN2543066Y CN2543066Y CN 02234988 CN02234988U CN2543066Y CN 2543066 Y CN2543066 Y CN 2543066Y CN 02234988 CN02234988 CN 02234988 CN 02234988 U CN02234988 U CN 02234988U CN 2543066 Y CN2543066 Y CN 2543066Y
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- CN
- China
- Prior art keywords
- chip
- protein
- aglucon
- covalent
- modified layer
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- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
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Abstract
The utility model relates to a protein chip which is covalent binding to ligand molecular on a solid surface. The chip comprises a solid substrate, a modified layer and a ligand inductive film, wherein, as the modified layer, the chlorosilane polyethylene glycol derivatives are respectively connected with the solid substrate and the ligand inductive film by a covalent bond. The protein chip of the utility model can effectively enhance the stability of the ligand molecular and inhibit the solid surface from adsorbing non-target protein, thereby reducing false positive result. The chip can be widely used in detection of all kinds of bimolecular such as antibody, antigen, receptor, ligand and DNA segment.
Description
Technical field
The utility model relates to the biological detection protein-chip, particularly a kind of can be on solid surface Covalent Immobilization aglucon molecule, and the effective protein-chip of the physisorption of Profilin matter molecule on chip surface.
Background technology
Protein-chip is a new technology utilizing aglucon molecule fixing on the chip surface to come the detection of biological molecule.Quality that the aglucon molecule is fixed on the protein-chip surface and chip surface are to influence the key factor that protein-chip detects to the absorption of other non-target protein.At present, the method that is used for fixing the aglucon molecule is physisorphtion mostly, as ELISA, and the fixing means that adopts in the detection methods such as RIA.Be fixed on the poor stability of the aglucon molecule on the chip surface by physisorphtion, easily come off, and easily cause the aglucon molecule degeneration, lose biologically active.In addition, use physisorphtion fixedly easily to adsorb non-target protein on the protein-chip surface of aglucon molecule, cause false positive results.
Summary of the invention
The purpose of this utility model is, overcome above-mentioned by the physisorption method fixedly the protein-chip of aglucon molecule bring aglucon molecule instability, easily come off from chip, the shortcoming of changeableness, and in order to eliminate the easily non-target protein absorption of generation of chip surface, and problems such as false positive testing result occur, thereby provide the protein-chip of covalent bond aglucon molecule on a kind of solid surface
The purpose of this utility model is to realize like this.
The protein-chip of the covalent bond aglucon molecule that the utility model provides comprises solid substrate, modified layer and aglucon sensor film three parts; Wherein modified layer is between solid substrate and aglucon sensor film, and modified layer is connected with the aglucon sensor film with solid substrate respectively by covalent bond.
Wherein said solid substrate is semiconductor material (as silicon chip, germanium wafer etc.), metal, glass, plastics or solid composite material (is the solid composite material of metal film, deielectric-coating, chemical films or biological chemistry film as the surface).
The aglucon sensor film is formed by the biomolecule that is used to detect, as various antibody, antigen, acceptor, part, dna fragmentation etc.
The modified layer of protein-chip is made of chloro-silicane polyethylene derivative.
Chloro-silicane polyethylene derivative molecule Cl
aSi (CH
2)
m(OCH
2CH
2)
nOX is by chlorosilane, alkane, and polyglycol, end group X four parts constitute.The a of chlorosilane part can be 1,2 or 3, i.e. a chlorosilane (ClSi-), dichlorosilane (Cl
2Si-) or trichlorosilane (Cl
3Si-).The polymerization degree n of polyglycol can select n between 3-8 between 3 to 50 usually.Chlorosilane part easily with solid surface on hydroxyl reaction generation silicon oxygen bond, thereby this compound covalently is fixed on the solid surface.Having of paraffin section benefits the orderly arrangement from the teeth outwards of chloro-silicane polyethylene derivative molecule.The water wettability of peg molecule, flexibility and electric neutrality be the physisorption of Profilin matter molecule on chip surface effectively.According to whether being used for Covalent Immobilization aglucon molecule, end group X is divided into two classes: a class is the group that can be used for fixing the aglucon molecule, as carboxyl, amino, sulfydryl, aldehyde radical etc., because these groups are easy and chlorosilane generation chemical reaction, so before being used to fixedly the aglucon molecule, taked different chemoproections according to different groups; Another kind of is inertia group, be not used for Covalent Immobilization aglucon molecule, as methyl, ethyl etc., the physical absorption effect of derivant Profilin matter molecule that has this class inert terminal group is better than the derivant that has the reactive terminal group, but inertia group can't activate in conjunction with the aglucon molecule.Therefore, that uses that proper ratio mixes has reactive group and has the protein-chip surface of the polyethyleneglycol derivative modification of inertia group, not only can Covalent Immobilization aglucon molecule, and can realize that farthest Profilin matter molecule is in the lip-deep physisorption of protein-chip.
The protein-chip that the utility model provides can be used for the aspects such as detection in life science biomolecule detection, clinical disease diagnosis and the biological industry production run.
Advantage of the present utility model:
1. the protein-chip that provides of the utility model can Covalent Immobilization aglucon molecule, makes it stable, difficult drop-off.
2. the protein-chip that provides of the utility model can keep the aglucon molecular biological activity, not the changeableness inactivation.
3. the protein-chip that provides of the utility model can suppress the physisorption of non-target protein molecule, avoids occurring
The false positive testing result.
Description of drawings
Fig. 1 is the protein-chip structural representation of the covalent bond aglucon molecule of embodiment 1 of the present utility model;
Drawing is described as follows:
1: solid substrate 2: modified layer 3: the aglucon sensor film
Embodiment
Embodiment 1
Press Fig. 1 preparation at the fixing protein-chip of immunoglobulin G while (IgG) antibody (antiIgG) of silicon chip surface.This protein-chip structure comprises as shown in Figure 1: the polished silicon slice that the integrated circuit that 0.5mm is thick is used is as solid substrate 1, covalently bound modified layer 2 on silicon chip 1, and modified layer 2 is by chloro-silicane polyethylene derivative Cl (CH
3)
2Si (CH
2)
11(OCH
2CH
2)
3OCH
2COOCH
2CH
3And Cl (CH
3)
2Si (CH
2)
11(OCH
2CH
2)
3OCH
2CH
3Constitute.Covalently bound aglucon sensor film 3 on the modified layer 2, this aglucon sensor film are immunoglobulin G while antibody (antiIgG).
The directed fixedly protein-chip of antiIgG in the present embodiment is immersed in the solution to be measured,, will combine, form compound molecule with the antiIgG specificity of chip aglucon sensor film if contain IgG in the solution.Whether rete changes, and can learn methods such as imaging method, surface plasma resonance by ellipse polarisation and detect.
At the fixing protein-chip of actrapid monotard's antibody (antiinsulin) of silicon chip surface.
This protein-chip comprises: the polished silicon slice that the integrated circuit that 0.5mm is thick is used, surface be the silica coating that generates of nature as fixed substrate 1, covalently bound modified layer 2 on silicon chip 1, it is by chloro-silicane polyethylene derivative Cl
2CH
3Si (CH
2)
9(OCH
2CH
2)
4OCH
2COOCH
2CH
3And Cl
2CH
3Si (CH
2)
9(OCH
2CH
2)
3O CH
3Constitute, covalently bound aglucon sensor film 3 on the modified layer 2, this aglucon sensor film are actrapid monotard's antibody (antiinsulin).
Embodiment 3
At the fixing protein-chip of human interleukin 6 (IL-6) of glass sheet surface.This protein-chip comprises: the thick polished glass sheet of 0.5mm is as fixed substrate 1, covalently bound modified layer 2 on silicon chip 1, and it is by chloro-silicane polyethylene derivative Cl
3Si (CH
2)
12(OCH
2CH
2)
5O NH
4(chemoproection of reactive group amino) and Cl
3Si (CH
2)
12(OCH
2CH
2)
3O CH
3Constitute, covalently bound aglucon sensor film 3 on the modified layer 2, this aglucon sensor film are human interleukin 6 (IL-6).
Embodiment 4
At the fixing protein-chip of human serum albumins (HSA) of the silicon chip surface of gold-plated film.
This protein-chip comprises: the silicon chip of the gold-plated film that 0.5mm is thick is as fixed substrate 1, covalently bound modified layer 2 on silicon chip 1, and it is by chloro-silicane polyethylene derivative Cl
3Si (CH
2)
12(OCH
2CH
2)
5OH (chemoproection of reactive group hydroxyl) and Cl
3Si (CH
2)
12(OCH
2CH
2)
3O CH
3Constitute, covalently bound aglucon sensor film 3 on the modified layer 2, this aglucon sensor film are human serum albumins (HSA).
Claims (6)
1. the protein-chip of a covalent fixing biomolecular, comprise solid substrate and aglucon sensor film, it is characterized in that: also comprise one deck modified layer between solid substrate and aglucon sensor film, modified layer is connected with the aglucon sensor film with solid substrate respectively by covalent bond.
2. by the protein-chip of the described covalent fixing biomolecular of claim 1, it is characterized in that described solid substrate is the solid composite material of semiconductor material, metal, glass, plastics or surface coating.
3. by the protein-chip of the described covalent fixing biomolecular of claim 2, it is characterized in that the solid composite material of described surface coating is: the surface is the solid composite material of metal film, deielectric-coating, chemical films or biological chemistry film.
4. by the protein-chip of the described covalent fixing biomolecular of claim 1, it is characterized in that described aglucon sensor film is to be made of the biomolecule that is used to detect.
5. by the protein-chip of the described covalent fixing biomolecular of claim 1, it is characterized in that described modified layer is by chloro-silicane polyethylene derivative Cl
aSi (CH
2)
m(OCH
2CH
2)
nOX and Cl
aSi (CH
2)
m(OCH
2CH
2)
nOCH
3Constitute, wherein a is 1,2 or 3, and the degree of polymerization can be any positive integer between 5 to 20, and n can be any positive integer between 3 to 50, and the chemical group X that is used for fixing the aglucon molecule is carboxyl, amino, sulfydryl or aldehyde radical.
6. by the protein-chip of the described covalent fixing biomolecular of claim 5, it is characterized in that n is any positive integer between 3 to 8, m is any positive integer between 8 to 12.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 02234988 CN2543066Y (en) | 2002-05-29 | 2002-05-29 | Protein chip of covalent stationary biomolecule |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 02234988 CN2543066Y (en) | 2002-05-29 | 2002-05-29 | Protein chip of covalent stationary biomolecule |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN2543066Y true CN2543066Y (en) | 2003-04-02 |
Family
ID=33709630
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 02234988 Expired - Lifetime CN2543066Y (en) | 2002-05-29 | 2002-05-29 | Protein chip of covalent stationary biomolecule |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN2543066Y (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101544807B (en) * | 2009-04-30 | 2012-05-30 | 中国科学技术大学 | Epoxy anti-fouling material and preparation method and use of same |
| CN102640000A (en) * | 2009-09-15 | 2012-08-15 | 国立鲁昂大学 | Improved method for a highly sensitive detection and quantification of biomolecules using secondary ion mass spectrometry (SIMS) |
-
2002
- 2002-05-29 CN CN 02234988 patent/CN2543066Y/en not_active Expired - Lifetime
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101544807B (en) * | 2009-04-30 | 2012-05-30 | 中国科学技术大学 | Epoxy anti-fouling material and preparation method and use of same |
| CN102640000A (en) * | 2009-09-15 | 2012-08-15 | 国立鲁昂大学 | Improved method for a highly sensitive detection and quantification of biomolecules using secondary ion mass spectrometry (SIMS) |
| CN102640000B (en) * | 2009-09-15 | 2016-01-20 | 碧欧西蒙斯科技公司 | Adopt improving one's methods of secondary ion mass spectrometry (SIMS) high-sensitivity detection and quantitative biomolecule |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| AV01 | Patent right actively abandoned |
Effective date of abandoning: 20050921 |
|
| AV01 | Patent right actively abandoned |
Effective date of abandoning: 20050921 |
|
| C25 | Abandonment of patent right or utility model to avoid double patenting |