CN2413783Y - Polyacrylamide gel horizontal cataphoresis bath - Google Patents
Polyacrylamide gel horizontal cataphoresis bath Download PDFInfo
- Publication number
- CN2413783Y CN2413783Y CN 99238245 CN99238245U CN2413783Y CN 2413783 Y CN2413783 Y CN 2413783Y CN 99238245 CN99238245 CN 99238245 CN 99238245 U CN99238245 U CN 99238245U CN 2413783 Y CN2413783 Y CN 2413783Y
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- cover plate
- polyacrylamide gel
- gel
- horizontal
- utility
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Abstract
The utility model relates to a polyacrylamide gel cataphoresis tank, particularly a horizontal gel-making tank body. After two shimming strips and a cover plate are added to the interior of a common horizontal cataphoresis gel-making tank body, the smooth and uniform polyacrylamide gel amine which is suitable for horizontal electrophoresis can be made. The thickness of the polyacrylamide gel prepared by the utility model can be changed by exchange of the shimming strips with different thicknesses; a narrow gap on the transverse direction of the cover plate is convenient for pouring the gel and placing a sample hole comb, and exhausting holes on four corners of the cover plate are favorable to air elimination in the gel-making process. When the utility model is applied, the smooth and uniform polyacrylamide gel which has consistent thickness and is suitable for the horizontal electrophoresis can be made.
Description
The utility model relates to a kind of polyacrylamide gel horizontal strip electrophoresis groove, particularly horizontal glue cell body.
Polyacrylamide gel electrophoresis is one of main separation and purification means of biomacromolecule materials such as protein, nucleic acid.Polyacrylamide gel electrophoresis technology at present commonly used is the method that Davis in 1964 and Orstein are used for serum analysis.This method is the alkaline system of a high pH (pH 8-9), and promptly whole electrophoresis process is finished in high pH value alkaline system.This method only is used for serum analysis albumen at first, found afterwards that it also was suitable for separating and purifying of other acidity and neutral protein matter, and the protein in the biomass cells all is acidity or neutral protein matter usually, therefore in this alkaline system, can both be dissociated into negatively charged ion and anode moves, thereby obtain separating and purifying, thereby this method uses till today always, and becomes the classical way of biomacromolecule materials such as separation and purification protein and nucleic acid.
Polyacrylamide gel electrophoresis is realized by electrophoresis chamber.Electrophoresis chamber can be divided into horizontal strip electrophoresis groove and vertical electrophoresis groove, be used for polyacrylamide and have only the vertical electrophoresis groove at present by electrophoretic electrophoresis chamber, major cause has following 2 points: the one, and the protein in the cell mostly is acidity or neutral greatly as previously mentioned, utilization vertical electrophoresis groove substantially can be with they separation and purification, thereby seldom consider to use the horizontal strip electrophoresis groove in the past; The 2nd, because the singularity of polyacrylamide gel, adopt existing horizontal strip electrophoresis groove can not prepare the gel of smooth homogeneous, and the smooth homogeneous of gel is the prerequisite of polypropylene amide gel electrophoresis, thereby adopts existing horizontal strip electrophoresis groove can not implement polyacrylamide gel electrophoresis.
Along with going deep into of scientific research, find biomass cells, particularly also exist a lot of basic proteins in some biotechnology mycetocyte, and be that acidity, neutrality and basic protein are present in the same cell jointly under many circumstances, adopt these three kinds of protein of polyacrylamide gel electrophoresis technology separation and purification simultaneously as desire, existing vertical electrophoresis groove or horizontal strip electrophoresis groove can not realize that all a kind of novel horizontal strip electrophoresis groove must be arranged.
The purpose of this utility model is that a kind of horizontal strip electrophoresis groove that is suitable for polyacrylamide gel electrophoresis, particularly horizontal glue cell body will be provided.
The utility model is achieved in that the filler strip of placing two length, thickness unanimity in the left and right sides of the bottom land longitudinal direction of common horizontal strip electrophoresis groove glue cell body, a cover plate is placed on the filler strip again, and the size of the size of cover plate and glue cell body matches.A horizontal narrow slit is arranged on the cover plate, supply injecting glue and put the sample well comb to use; In the position near four angles of cover plate a venting hole is arranged respectively, exhaust is used during for injecting glue.Cover plate can make the smooth homogeneous of the gel of preparation, and filler strip can be guaranteed made gel thicknesses unanimity, but also can change filler strip as required, with the gel of preparation different thickness.
The utility model only needs to add two filler strips and a cover plate in common horizontal strip electrophoresis groove glue cell body, so structure is simple especially.Simultaneously the thickness of gel can obtain adjusting by changing filler strip, and the eliminating of the air when helping glue of the venting hole on the cover plate, the trouble of emptying gas when having avoided glue.
Concrete structure of the present utility model is provided by following embodiment and accompanying drawing thereof.
Fig. 1-Fig. 4 is respectively the vertical view according to basic view, transverse cross-sectional view, longitudinal sectional drawing and the cover plate of the glue cell body that the utility model proposes.
The details and the working condition of the concrete device that provides according to the utility model are provided in detail below in conjunction with these figure.This device comprises a glue cell body (1), and a cover plate (2) is arranged in the cell body, two filler strips (3) and two baffle plates (4).During glue filler strip (3) is put into glue cell body (1), cover plate (2) is positioned on the filler strip (3), two baffle plates (4) place the two ends of cell body respectively, then glue is injected in the space that is formed by glue cell body (1), cover plate (2) and baffle plate (4) by the narrow slit (5) on the cover plate (2), the venting hole (6) at four angles of cover plate plays scavenging action when injecting glue.After glue is filled with, sample well comb (7) is carefully inserted in the narrow slit (5), what so just can make smooth homogeneous is suitable for the electrophoretic polyacrylamide gel of horizontal strip electrophoresis groove.
Claims (3)
1. polyacrylamide gel horizontal strip electrophoresis groove, particularly horizontal glue slot device, this device comprise a glue
Cell body (1) has a cover plate (2) in the cell body, two filler strips (3) and two baffle plates (4) is characterized in that filler strip
(3) place in the glue cell body (1), cover plate (2) is positioned on the filler strip (3), and two baffle plates (4) place cell body respectively
Two ends.
2. device according to claim 1 is characterized in that on four angles of cover plate (2) four venting holes being arranged respectively
(6)。
3. device according to claim 1 is characterized in that a horizontal narrow slit (5) is arranged on the cover plate (2).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 99238245 CN2413783Y (en) | 1999-08-05 | 1999-08-05 | Polyacrylamide gel horizontal cataphoresis bath |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 99238245 CN2413783Y (en) | 1999-08-05 | 1999-08-05 | Polyacrylamide gel horizontal cataphoresis bath |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN2413783Y true CN2413783Y (en) | 2001-01-10 |
Family
ID=34025720
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 99238245 Expired - Fee Related CN2413783Y (en) | 1999-08-05 | 1999-08-05 | Polyacrylamide gel horizontal cataphoresis bath |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN2413783Y (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101184991B (en) * | 2005-04-29 | 2011-12-21 | 贝克顿·迪金森公司 | Method for electrophoresis involving parallel and simultaneous separation |
-
1999
- 1999-08-05 CN CN 99238245 patent/CN2413783Y/en not_active Expired - Fee Related
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101184991B (en) * | 2005-04-29 | 2011-12-21 | 贝克顿·迪金森公司 | Method for electrophoresis involving parallel and simultaneous separation |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C19 | Lapse of patent right due to non-payment of the annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |