CN2394917Y - Rotary centrifugal column for nucleic acid - Google Patents
Rotary centrifugal column for nucleic acid Download PDFInfo
- Publication number
- CN2394917Y CN2394917Y CN 99225683 CN99225683U CN2394917Y CN 2394917 Y CN2394917 Y CN 2394917Y CN 99225683 CN99225683 CN 99225683 CN 99225683 U CN99225683 U CN 99225683U CN 2394917 Y CN2394917 Y CN 2394917Y
- Authority
- CN
- China
- Prior art keywords
- film
- nucleic acid
- column
- cylinder
- rotary centrifugal
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
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- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
The utility model relates to a purified molecular biology product for nucleic acid, which can be used for a plurality of researches and clinical detection for the molecular biology. The rotary centrifugal column comprises a column body, a gasket, a film and a shim, wherein, the column body is hollow and the gasket, the film and the shim are orderly arranged inside the column from top to bottom; when the rotary centrifugal column is used, the column body is inserted on a liquid-collecting pipe and the film generally adopts one to three layers. The utility model has the advantages of simple structure, convenient operation, high speed and high product purity, and is an ideal appliance for modern genetic engineering.
Description
The utility model relate to a kind of nucleic acid (DNA and RNA) purifying the molecular biology product, can be directly used in multiple molecular biology research and clinical detection.
Traditional nucleic acid purification method such as DNA extraction mainly adopt the cell pyrolysis liquid lysing cell, discharge nucleus, behind protease K digesting histone, released dna, use the method for phenol extraction and alcohol precipitation at last.Adopt the said extracted method, complex steps, length consuming time, extraction yield is low, and repeatability is relatively poor, needs through the resolution of precipitate step some reagent also be corrosive (as phenol).
The purpose of this utility model is to avoid the defective of prior art and a kind of easy and simple to handle, rapid, nucleic acid purifying rotating centrifugal post that purification degree is high is provided.
Goal of the invention of the present utility model is achieved by the following scheme: this rotating centrifugal post inserts the cylinder in adapter when comprising adapter and use, cylinder hollow is provided with packing ring, film, pad from top to down successively in the cylinder.
Described cylinder bottom can be funnel-form, and packing ring, film, pad are located at the bottom inlet place.
Described film can be made up of the 1-3 layer.
The utility model adopts rotating centrifugal column technology purifying nucleic acid (DNA or RNA), and is easy and simple to handle, rapid, the purification degree height, and also this centrifugal rod structure is simple, and cost is low, is the desirable articles for use of modern genetic engineering.
Description of drawings:
Fig. 1 is a structural representation of the present utility model
Shown in Figure 1, the nucleic acid purifying rotating centrifugal post inserts the cylinder 1 in adapter 5 when comprising adapter 5 and use, cylinder 1 hollow, and its bottom is funnel-form, and the bottom inlet place is placed with packing ring 2, film 3, pad 4 from top to down successively, and the three cooperates one.Cylinder 1, packing ring 2 are all made with the ABS material, and film is adsorption of DNA or RNA optionally, according to different requirements, in the cylinder 1 multilayer film can be housed, and can adopt the 1-3 tunic usually.The external diameter of cylinder 1 cooperates with the internal diameter of adapter 5 under a stable condition, high-speed motion that can be by whizzer with solution from cylinder 1 in going into adapter 5, nucleic acid (DNA or RNA) then is adsorbed on the film 3.
When sample after pre-treatment, add in the centrifugal post, by centrifugal, DNA or RNA under certain condition can be by efficiently, be adsorbed on the film specifically, and most impurity all can by film by centrifugal to adapter.Remove through the centrifugal trace impurity (things such as divalent cation and some foreign proteins) that will remain on the film of twice flushing again.Wash highly purified DNA or RNA with less salt neutral buffered liquid at last.The utility model can be from multiple mark bodies such as blood, urine, tissue juice, ight soil, tissue highly purified DNA of rapid extraction or RNA, can be directly used in multiple molecular biology research and clinical detection.Mainly comprise: microorganism detection, virus detect, inherited disease detects, cancer research, forensic analysis, endonuclease reaction, HLA somatotype, RT reaction, rflp analysis, determined dna sequence, transgene clone screening.
Claims (3)
1. a nucleic acid purifying rotating centrifugal post inserts the cylinder (1) in adapter (5) when comprising adapter (5) and use, and it is characterized in that: cylinder (1) hollow is provided with packing ring (2), film (3), pad (4) from top to down successively in the cylinder (1).
2. rotating centrifugal post as claimed in claim 1 is characterized in that: described cylinder (1) bottom is funnel-form, and packing ring (2), film (3), pad (4) are located at the bottom inlet place.
3. rotating centrifugal post as claimed in claim 1 is characterized in that: described film (3) is made up of the 1-3 layer.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 99225683 CN2394917Y (en) | 1999-02-03 | 1999-02-03 | Rotary centrifugal column for nucleic acid |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 99225683 CN2394917Y (en) | 1999-02-03 | 1999-02-03 | Rotary centrifugal column for nucleic acid |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN2394917Y true CN2394917Y (en) | 2000-09-06 |
Family
ID=34015507
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 99225683 Expired - Lifetime CN2394917Y (en) | 1999-02-03 | 1999-02-03 | Rotary centrifugal column for nucleic acid |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN2394917Y (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109207340A (en) * | 2017-06-30 | 2019-01-15 | 开启基因股份有限公司 | The fixing pipe and nucleic acid extraction component of nucleic acid extraction component |
| WO2020211637A1 (en) * | 2019-04-19 | 2020-10-22 | 安徽森芃生物科技有限责任公司 | Method for purifying and concentrating dna in forensic samples using selective filtration column |
-
1999
- 1999-02-03 CN CN 99225683 patent/CN2394917Y/en not_active Expired - Lifetime
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109207340A (en) * | 2017-06-30 | 2019-01-15 | 开启基因股份有限公司 | The fixing pipe and nucleic acid extraction component of nucleic acid extraction component |
| WO2020211637A1 (en) * | 2019-04-19 | 2020-10-22 | 安徽森芃生物科技有限责任公司 | Method for purifying and concentrating dna in forensic samples using selective filtration column |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C17 | Cessation of patent right | ||
| CX01 | Expiry of patent term |