CN221281047U - Automatic pretreatment system for reagent - Google Patents

Abstract

The application discloses an automatic reagent pretreatment system, which comprises a material storage component, a motion carrying component, a moving line component and an automatic sample adding component, wherein corresponding materials and reagents are stored by the material storage component, and the transfer of a micro-pore plate component and the transfer of a sample sucking plate are automatically completed by the motion carrying component; the micro-pore plate assembly can be placed on a supporting moving platform of the moving line assembly, is driven by the linear moving module to linearly move, sucks samples through a sample sucking plate when moving to the sample sucking platform, and heats and dries when moving to the drying platform; when the micro-pore plate assembly is positioned at the sample dripping station, the sample adding gun absorbs the reagent from the reagent placing bin and drips the reagent to the micro-pore plate assembly; the transfer, sample adding, drying and sample sucking processes required by the whole pretreatment process are realized by mechanical automation, so that the automation degree of pretreatment operation is improved, and the injury to operators can be avoided.

Description

Automatic pretreatment system for reagent

Technical Field

The application relates to the technical field of mechanical arm sample adding, in particular to an automatic reagent pretreatment system.

Background

In various scientific fields such as biomedicine, chemical industry and the like, pretreatment operations such as sample loading, sample drying, liquid sample suction and discarding of a plurality of points and the like are involved. At present, a pipettor is generally used for sample feeding, natural drying and water absorption paper for sample absorption and rejection and manual transfer, so that the treatment time is long and the working efficiency is low; moreover, due to uneven technical level of operators, the whole sample treatment process lacks standardized and normalized operation management, and the repeatability and consistency of the manual flow treatment of the samples cannot be ensured; more importantly, many samples contain toxic and corrosive components, are easy to corrode skin and stimulate respiratory systems, and have potential safety hazards.

In view of the above technical problems, how to improve the automation degree of the pretreatment operation is a technical problem to be solved by those skilled in the art.

Disclosure of utility model

The application aims to provide an automatic reagent pretreatment system which can automatically complete pretreatment operation and improve automation level.

To achieve the above object, the present application provides an automatic reagent pretreatment system comprising:

The material storage assembly comprises a suction head placing bin, a micro-pore plate storing bin, a suction plate storing bin, a waste bin and a reagent placing bin;

the moving and carrying assembly comprises a carrying mechanical arm and clamping jaws, the carrying mechanical arm drives the clamping jaws to move, and the clamping jaws can clamp the micro-pore plate assembly from the micro-pore plate storage bin, clamp the suction plate from the suction plate storage bin and transfer the suction plate to the waste bin;

The mobile line assembly comprises a linear mobile module, a supporting mobile platform, a sample sucking platform and a drying platform, wherein the linear mobile module can drive the supporting mobile platform to linearly move, the supporting mobile platform further drives the micro-pore plate assembly to pass through the sample sucking platform and the drying platform, the sample sucking platform sucks samples through a sample sucking plate, and the drying platform heats and dries;

The automatic sample adding assembly comprises a sample adding mechanical arm and a sample adding gun, wherein the sample adding mechanical arm drives the sample adding gun to move, gun suction heads are obtained from the suction head placing bin, and reagents are sucked from the reagent placing bin and added to the micro-pore plate assembly at the sample dropping station in a dropwise manner.

Preferably, the drying platform further comprises:

The drying bracket is arranged on the linear movement module;

a mounting housing secured to the drying rack;

A fan fixed inside the installation housing for blowing an air flow toward the support moving platform;

The heating plate is fixed in the installation shell and is used for heating air flow formed by the fan;

And the temperature fuse is used for high-temperature protection.

Preferably, the sample absorbing platform further comprises:

The sample suction bracket is used for placing a sample suction plate;

the sample sucking and jacking assembly is positioned below the sample sucking support and can penetrate through the support moving platform to jack up the micro-pore plate assembly to be in contact with the sample sucking plate.

Preferably, the sample sucking and lifting assembly comprises a lifting motor, a lifting screw rod, a lifting guide rail and a lifting plate, wherein the lifting motor drives the lifting screw rod to rotate so as to drive the lifting plate to vertically move along the lifting guide rail;

wherein, the upper surface of jacking board sets up guide pin and location magnet.

Preferably, the microplate assembly comprises a carrier and a planar microplate, and a stepped groove for clamping the planar microplate is formed in the upper surface of the carrier;

The upper surface of the supporting moving platform is provided with a conical guide column for guiding the micro-pore plate assembly.

Preferably, the carrier is provided with a fixed magnet, a positioning steel sheet and a guide groove, the positioning magnet can be in magnetic attraction fit with the positioning steel sheet, and the guide pin is in plug-in positioning fit with the guide groove;

And the supporting mobile platform is provided with a fixed steel sheet matched with the fixed magnet.

Preferably, the carrier is provided with a first hand avoiding position for taking out the planar microwell plate and a second hand avoiding position for clamping by the clamping jaw.

Preferably, the moving line assembly further comprises a secondary positioning assembly for positioning the micro-porous plate assembly, the secondary positioning assembly comprises a secondary positioning mounting plate, a linear guide rail, a limiting plate, a top block mounting plate, a top block, a guide screw and a spring, the linear guide rail is mounted on the secondary positioning mounting plate, the top block mounting plate can translate along the linear guide rail, and the top block is detachably mounted on the top block mounting plate; the guide screw is slidably assembled on the limiting plate, and the spring is used for applying elastic force to the top block mounting plate;

The top block is provided with at least two conical blocks which are matched with the guide conical grooves arranged on the micro-pore plate assembly for insertion and positioning.

Preferably, the microplate storage bin and the suction plate storage bin are respectively provided with a feeding jacking assembly, and the feeding jacking assemblies are used for jacking up layer by layer.

Preferably, the handling mechanical arm and the sample loading mechanical arm are respectively slidably mounted on a moving sliding table, and the moving sliding table is used for driving the handling mechanical arm and the sample loading mechanical arm to move along an X axis.

Preferably, the sampling mechanical arm comprises a Y-axis sampling assembly and a Z-axis sampling assembly, wherein the Y-axis sampling assembly is used for driving the Z-axis sampling assembly to move along a Y axis;

The Z-axis sample adding assemblies are arranged in two groups, and each Z-axis sample adding assembly correspondingly drives one sample adding gun to move along the Z axis.

Preferably, the carrying mechanical arm comprises a Y-axis carrying assembly and a Z-axis carrying assembly, wherein the Y-axis carrying assembly is used for driving the Z-axis carrying assembly to move along a Y-axis;

The clamping jaw is rotatably installed in the Z-axis carrying assembly, and the Z-axis carrying assembly is used for driving the clamping jaw to move along the Z axis and can rotate around the Z axis.

Preferably, the handling mechanical arm further comprises a pressing plate installed on the Z-axis handling assembly, and the Z-axis handling assembly drives the pressing plate to move along the Z-axis so as to press on a sample suction plate placed on the sample suction platform.

Compared with the background technology, the automatic reagent pretreatment system stores corresponding materials and reagents through the material storage component, and automatically completes the transfer of the micro-pore plate component and the transfer of the suction plate through the motion carrying component; the micro-pore plate assembly can be placed on a supporting moving platform of the moving line assembly, is driven by the linear moving module to linearly move, sucks samples through a sample sucking plate when moving to the sample sucking platform, and heats and dries when moving to the drying platform; when the micro-pore plate assembly is positioned at the sample dripping station, the sample adding gun absorbs the reagent from the reagent placing bin and drips the reagent to the micro-pore plate assembly; the transfer, sample adding, drying and sample sucking processes required by the whole pretreatment process are realized by mechanical automation, so that the automation degree of pretreatment operation is improved.

Drawings

In order to more clearly illustrate the embodiments of the present application or the technical solutions in the prior art, the drawings that are required to be used in the embodiments or the description of the prior art will be briefly described below, and it is obvious that the drawings in the following description are only embodiments of the present application, and that other drawings can be obtained according to the provided drawings without inventive effort for a person skilled in the art.

FIG. 1 is a schematic axial view of an overall structure of an automatic reagent pretreatment system according to an embodiment of the present application;

FIG. 2 is a front view of the overall structure of an automated reagent pretreatment system according to an embodiment of the present application;

FIG. 3 is a top view of the overall structure of an automated reagent pretreatment system according to an embodiment of the present application;

FIG. 4 is an isometric view of the overall structure of the moving wire assembly;

FIG. 5 is an isometric view of a linear motion module and a support motion platform;

FIG. 6 is an isometric view of the overall structure of the drying deck;

FIG. 7 is an overall structural elevation view of the drying deck;

FIG. 8 is an exploded view of the drying deck;

FIG. 9 is an isometric view of the overall structure of the sample ejection assembly;

FIG. 10 is an overall structural elevation view of the sample ejection assembly;

FIG. 11 is a top view of the overall construction of the sample ejection assembly;

FIG. 12 is a schematic cross-sectional view of a sample ejection assembly;

FIG. 13 is a schematic view of the overall structure of a microplate assembly;

FIG. 14 is an exploded view of the carrier;

FIG. 15 is a cross-sectional view of a carrier;

FIG. 16 is an exploded view of the secondary positioning assembly;

FIG. 17 is an isometric view of the overall structure of the secondary positioning assembly;

FIG. 18 is a schematic diagram of the automated loading assembly;

fig. 19 is a schematic view of the structure of the motion-carrying assembly.

In the figure: 1. the device comprises a material storage assembly 11, a suction head placing bin 12, a micro-pore plate storing bin 13, a suction plate storing bin 14, a waste bin 15, a reagent placing bin 16 and a reagent bottle;

2. A motion carrying assembly 21, a carrying mechanical arm 211, a Y-axis carrying assembly 212, a Z-axis carrying assembly 213, a pressing plate 22 and a clamping jaw;

3. The moving wire assembly 31, the linear moving module 32, the supporting moving platform 321, the conical guide post 33, the sample sucking platform 331, the sample sucking support 332, the sample sucking and lifting assembly 3321, the lifting motor 3322, the lifting screw 3323, the lifting guide rail 3324, the lifting plate 3325, the guide pins 3326, the positioning magnet 34, the drying platform 341, the drying support 342, the mounting housing 343, the fan 344, the heating plate 345, the temperature fuse 35, the secondary positioning assembly 351, the secondary positioning mounting plate 352, the linear guide 353, the limiting plate 354, the top block mounting plate 355, the top block 356, the guide screw 357, the spring 358 and the conical block;

4. An automatic sample adding component 41, a sample adding mechanical arm 411, a Y-axis sample adding component 412, a Z-axis sample adding component 42 and a sample adding gun;

5. Microplate assembly 51, carrier 511, fixed magnet 512, positioning steel sheet 513, guide groove 514, first hand-avoiding position 515, second hand-avoiding position 516, guide cone groove 52, planar microplate;

6. And moving the sliding table.

Detailed Description

The following description of the embodiments of the present application will be made clearly and completely with reference to the accompanying drawings, in which it is apparent that the embodiments described are only some embodiments of the present application, but not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the application without making any inventive effort, are intended to be within the scope of the application.

The present application will be further described in detail below with reference to the drawings and detailed description for the purpose of enabling those skilled in the art to better understand the aspects of the present application.

It should be noted that, in the present embodiment, the orientation or positional relationship indicated by "upper", "lower", "front", "rear", etc. is based on the orientation or positional relationship shown in the drawings, and is merely for convenience of describing the present application and simplifying the description, and does not indicate or imply that the device or element to be referred to must have a specific orientation, be configured and operated in a specific orientation, and therefore should not be construed as limiting the present application.

As shown in fig. 1 to 5, in the present embodiment, an automatic reagent pretreatment system is provided, which comprises a material storage component 1, a motion handling component 2, a moving line component 3 and an automatic sample adding component 4, wherein the material storage component 1 is used for storing materials and reagents, and comprises a suction head placing bin 11, a micro-pore plate placing bin 12, a suction plate placing bin 13, a waste bin 14 and a reagent placing bin 15, the suction head placing bin 11 is used for placing suction heads in an array, a sample adding gun 42 of the automatic sample adding component 4 can take out a disposable suction head from the suction head placing bin 11, and the operation of dripping liquid drops is completed by sucking the reagents through the suction head. The microplate storage bin 12 is used for accommodating unused microplate assemblies 5 therein, the upper surface of the microplate assemblies 5 can bear droplets, and the droplets are dripped into the microplate assemblies 5 through the sample application gun 42 to form arrayed droplets. The unused suction plate is placed in the suction plate storage bin 13, the suction plate is a flat consumable with micro-liquid suction capability, can suck liquid samples at a plurality of points at the same time, is a disposable consumable, and avoids cross contamination of the samples caused by repeated use of the consumable; the suction plate can absorb liquid when contacting the upper surface of the microplate assembly 5, and absorb the liquid on the microplate assembly 5. The waste bin 14 is used for storing used materials, and can store used suction heads and suction plates for unified collection treatment. The reagent placing bin 15 is used for placing a reagent to be used, and when the reagent is dripped into the micro-pore plate assembly 5, the suction head sucks the corresponding reagent from the reagent placing bin 15. Bottled reagent is placed in the reagent placing bin, and after the bottled reagent is configured by an operator, the bottled reagent is placed in the reagent placing bin.

The moving handling assembly 2 comprises a handling mechanical arm 21 and clamping jaws 22, wherein the handling mechanical arm 21 can drive the clamping jaws 22 to move so that the clamping jaws 22 move between different positions; the carrying mechanical arm 21 drives the clamping jaw 22 to clamp the micro-pore plate assembly 5 from the micro-pore plate storage bin 12, the micro-pore plate assembly 5 is placed on the supporting moving platform 32, the supporting moving platform 32 drives the micro-pore plate assembly 5 to linearly move, and the micro-pore plate assembly 5 is transferred to different stations to perform corresponding operations. The carrying mechanical arm 21 drives the clamping jaw 22 to clamp the suction template from the suction template storage bin 13, the suction template is placed on the suction platform 33, and when the micro-pore plate assembly 5 reaches a station corresponding to the suction platform 33, liquid drops on the micro-pore plate assembly 5 are sucked by the suction template. The handling mechanical arm 21 drives the clamping jaw 22 to transfer the suction sample plate to the waste bin 14, and after the suction sample plate is completed, the suction sample plate is disposable, and the suction sample plate is transferred to the waste bin 14 for collection. The operations described above with respect to the motion-carrying assembly 2 are only some specific embodiments, and the motion-carrying assembly 2 can perform actions not only in this regard, but also in other operations, and the above-described operations with respect to the motion-carrying assembly 2 are not limited to the only ones.

The moving line assembly 3 comprises a linear moving module 31, a supporting moving platform 32, a sample sucking platform 33 and a drying platform 34, wherein the linear moving module 31 comprises a guide rail and a driving assembly, and the driving assembly can adopt different forms such as a belt, a screw rod and the like; the support moving platform 32 is slidably mounted on the guide rail of the linear moving module 31, the linear moving module 31 can drive the support moving platform 32 to linearly move, the sample sucking platform 33 and the drying platform 34 are fixedly arranged, the sample sucking platform 33 and the drying platform 34 are located on the moving path of the support moving platform 32, and the support moving platform 32 drives the micro-pore plate assembly 5 to pass through the sample sucking platform 33 and the drying platform 34. When the sample is required to be sucked into the micro-pore plate assembly 5, the micro-pore plate assembly 5 stops at a station corresponding to the sample sucking platform 33, the sample sucking plate is placed on the sample sucking platform 33, the sample sucking plate contacts with the upper surface of the micro-pore plate assembly 5, and liquid drops on the upper surface of the micro-pore plate assembly 5 are sucked through the sample sucking plate. When the micro-pore plate assembly 5 needs to be dried, the micro-pore plate assembly 5 stops at a station corresponding to the drying platform 34, and the micro-pore plate assembly 5 is dried and heated through the drying platform 34, so that the drying process of the micro-pore plate assembly 5 is accelerated.

The automatic sample adding assembly 4 is used for completing the operation of dripping liquid reagent, and comprises a sample adding mechanical arm 41 and a sample adding gun 42, wherein the sample adding mechanical arm 41 drives the sample adding gun 42 to move, so that the sample adding gun 42 moves to different positions, and the suction head is driven to transfer between the reagent placing bin 15 and the micro-pore plate assembly 5, so that the reagent is dripped into the micro-pore plate assembly 5. When the reagent is required to be added dropwise, the linear moving module 31 drives the supporting moving platform 32 to move to the sample dropping station, the sample adding mechanical arm 41 drives the sample adding gun 42 to obtain gun suction heads from the suction head placing bin 11, the reagent is sucked from the reagent placing bin 15 and is dripped to the micro-pore plate assembly 5 positioned at the sample dropping station, and a plurality of liquid drops can be dripped on the upper surface of the micro-pore plate assembly 5 by sucking the primary sample through the suction heads, so that the liquid drops form array arrangement.

In summary, the automatic reagent pretreatment system of the present application uses the motion carrying assembly 2 to complete the transfer operation of the microplate assembly 5 and the suction plate, uses the motion line assembly 3 to carry and transfer the microplate assembly 5, performs pretreatment processes such as reagent dripping, suction, drying, etc. on the microplate assembly 5 at different stations, and automatically completes the transfer of the microplate assembly 5; the automatic sample adding assembly 4 is utilized to automatically complete the dripping process during the reagent dripping, so that the automation degree of pretreatment operation is improved, and the potential safety hazard caused by skin corrosion and respiratory system irritation due to toxic and corrosive components can be reduced as no manual participation is needed in the operation process.

In order to further optimize the above scheme, the drying platform 34 of the present application further includes a drying bracket 341, a mounting housing 342, a fan 343, a heating plate 344, a temperature fuse 345, and other structures, and referring to fig. 6 to 8, the drying bracket 341 is mounted on the linear movement module 31, the drying bracket 341 is used for providing a support, the drying bracket 341 is in a "door" frame, and the linear movement module 31 drives the supporting movement platform 32 and the micro-pore plate assembly 5 to pass under the drying bracket 341. The installation housing 342 is fixed to the drying bracket 341, the fan 343 and the heating sheet 344 are installed inside the installation housing 342, and the surface of the installation housing 342 is provided with a through hole for circulating air flow; a fan 343 is fixed in the installation housing 342 for blowing air flow toward the support moving platform 32, and when the fan 343 rotates, wind pressure is generated to form air flow toward the microplate assembly 5 to be dried; the heating sheet 344 is fixed inside the mounting housing 342, and is used for heating the air flow formed by the fan 343, so that the temperature of the air flow is increased, and the drying process is accelerated. The temperature fuse 345 is used for high temperature protection, and stops heating when the temperature is too high.

Further, the sample sucking platform 33 further comprises a sample sucking support 331 and a sample sucking jacking component 332, the sample sucking support 331 is used for placing a sample sucking plate, the sample sucking support 331 is a 'door' -shaped frame, a step groove for placing the sample sucking plate is formed in the upper surface of the sample sucking support 331, and the sample sucking plate is placed in the step groove to achieve positioning. The sample sucking and jacking assembly 332 is located below the sample sucking support 331, and the sample sucking and jacking assembly 332 can penetrate through the supporting and moving platform 32 to jack up the micro-pore plate assembly 5 upwards to contact the sample sucking plate, and when sample sucking is needed, the micro-pore plate assembly 5 is jacked up by the sample sucking and jacking assembly 332, so that the micro-pore plate assembly 5 contacts the sample sucking plate to realize sample sucking.

Specifically, referring to fig. 9 to 12, the sample ejection assembly 332 includes an ejection motor 3321, an ejection screw 3322, an ejection guide rail 3323, and an ejection plate 3324, where the ejection motor 3321 drives the ejection screw 3322 to rotate, and the ejection guide rail 3323 provides a guiding function for the ejection plate 3324, and the ejection screw 3322 drives the ejection plate 3324 to move vertically along the ejection guide rail 3323. The screw rod structure provided in this embodiment is only a preferred solution, and other vertical driving structures may be adopted.

Further, the upper surface of the jacking plate 3324 is provided with guide pins 3325 and positioning magnets 3326, at least two guide pins 3325 are arranged, the guide pins 3325 protrude out of the upper surface of the jacking plate 3324, and the guide pins 3325 can be matched with guide grooves 513 arranged on the lower surface of the microplate assembly 5 for insertion and positioning. The positioning magnet 3326 is used for adsorbing the positioning steel sheet 512 arranged on the lower surface of the micro-pore plate assembly 5 when lifting upwards, and plays a certain role in fixing.

Specifically, referring to fig. 8 to 10, the microplate assembly 5 includes a carrier 51 and a planar microplate 52, and a stepped groove for clamping the planar microplate 52 is provided on an upper surface of the carrier 51, and the stepped groove can exactly accommodate the planar microplate 52 and play a role in positioning the planar microplate 52 circumferentially. The upper surface that supports mobile platform 32 sets up the toper guide post 321 that is used for leading microplates subassembly 5, the diameter of toper guide post 321 from the top down increases gradually, toper guide post 321 sets up the round under the general circumstances, every side of microplates subassembly 5 corresponds two toper guide posts 321 respectively, also set up eight toper guide posts 321 in total, when microplates subassembly 5 from the top down place, toper guide post 321 can allow certain error, contact toper guide post 321 when microplates subassembly 5 whereabouts, further adjust the position of microplates subassembly 5, make microplates subassembly 5 place in supporting mobile platform 32 more accurately.

Further, the carrier 51 is provided with a fixed magnet 511, a positioning steel sheet 512 and a guiding groove 513, wherein the positioning steel sheet 512 and the guiding groove 513 are arranged on the lower surface of the carrier 51, and at least two fixed magnets 511 are arranged; preferably, a plurality of grooves are arranged on the carrier 51, each groove is correspondingly provided with a fixed magnet 511, and a stainless steel sheet is packaged to shield the fixed magnet 511. The positioning magnet 3326 arranged on the jacking plate 3324 can be magnetically matched with the positioning steel sheet 512, the guide pin 3325 is matched with the guide groove 513 for insertion positioning, the guide pin 3325 is a conical projection, the guide groove 513 is a conical groove, when the jacking plate 3324 moves upwards and contacts the upper surface of the carrier 51, the guide pin 3325 is inserted into the guide groove 513 to realize alignment, and the positioning magnet 3326 is magnetically matched with the positioning steel sheet 512. The fixed steel sheet matched with the fixed magnet 511 is arranged on the supporting mobile platform 32, so that the carrier 51 is always clung to the upper surface of the supporting mobile platform 32 in the moving process of the supporting mobile platform 32, and the shaking phenomenon is prevented.

Preferably, the carrier 51 is provided with a first hand-avoiding position 514 for taking out the planar microplate 52 and a second hand-avoiding position 515 for clamping by the clamping jaw 22, wherein the first hand-avoiding position 514 is a notch disposed on at least one side of the carrier 51, and it should be noted that the first hand-avoiding position 514 is generally located at a middle position of the side of the carrier 51, but is not excluded from being disposed at other positions. The first hand avoiding position 514 has no physical structure, so when the planar micro-plate 52 needs to be removed from the carrier 51, one finger passes through the first hand avoiding position 514 to contact the lower surface of the planar micro-plate 52, and the other finger presses on the upper surface of the planar micro-plate 52, so that the planar micro-plate 52 can be removed from the carrier 51. The second keeps away the position 515 and sets up two at least to the second keeps away the position 515 relatively and sets up, and the second keeps away the position 515 can be the ladder groove, also can be the breach, and two clamping jaws 22 can contact a second respectively keeps away the position 515 to centre gripping carrier 51 makes the centre gripping of clamping jaw 22 more stable through two second keeps away the position 515, conveniently takes when carrier 51 places at desktop or plane.

Still further, the moving wire assembly 3 further includes a secondary positioning assembly 35 for positioning the micro-porous plate assembly 5, and as shown in fig. 11 and 12, the secondary positioning assembly 35 includes a secondary positioning mounting plate 351, a linear guide 352, a limiting plate 353, a top block mounting plate 354, a top block 355, a guide screw 356, and a spring 357; the secondary positioning component 35 is used for realizing secondary positioning on the micro-pore plate component 5, the primary positioning process is realized by the conical guide post 321 arranged on the supporting moving platform 32, the secondary positioning is realized by the secondary positioning component 35, and the micro-pore plate component 5 contacts with the secondary positioning component 35 when reaching the sample dripping station. The secondary positioning mounting plate 351 plays a role in fixing, the secondary positioning mounting plate 351 is fixed at the end part of the linear movement module 31, the linear guide rail 352 is mounted on the upper surface of the secondary positioning mounting plate 351, the linear guide rail 352 plays a role in guiding, and the length direction of the linear guide rail 352 is parallel to the length direction of the linear movement module 31; the top block mounting plate 354 is translatable along the linear guide 352, the direction of movement of the top block mounting plate 354 being parallel to the direction of movement of the microplate assembly 5. The ejector block 355 is detachably mounted on the ejector block mounting plate 354, and the ejector block 355 and the ejector block mounting plate 354 are designed in a split mode, so that when the ejector block 355 is worn, replacement of the ejector block 355 can be conveniently performed. The limiting plate 353 is vertically fixed on the upper surface of the secondary positioning mounting plate 351, a guide through hole for sliding and assembling the guide screw 356 is arranged on the limiting plate 353, the guide screw 356 is slidably assembled on the guide through hole of the limiting plate 353, the spring 357 is used for applying elastic force to the top block mounting plate 354, the spring 357 can be sleeved on the periphery of the guide screw 356, and the spring 357 generates elastic force between the top block mounting plate 354 and the limiting plate 353. The kicking block 355 sets up two at least and the guide cone groove 516 cooperation cartridge that sets up on the microplates subassembly 5 and fix a position toper piece 358, toper piece 358 protrusion in the lateral wall of kicking block 355, toper piece 358 orientation microplates subassembly 5, when support moving platform 32 drive microplates subassembly 5 and remove to arrive the sample station of dripping towards secondary positioning subassembly 35, microplates subassembly 5 contact kicking block 355, and the guide cone groove 516 of microplates subassembly 5 matches the grafting with toper piece 358 and realizes the secondary positioning. When the micro-pore plate assembly 5 impacts the conical block 358, the spring 357 is compressed, and the function of the spring 357 is that when the micro-pore plate assembly 5 is propped up, a certain compression amount exists, so that the conical block 358 on the top block 355 can be effectively guaranteed to be closely attached to the two inwards-sunken guide conical grooves 516 on the micro-pore plate assembly 5, and the guide precision is guaranteed. When the microplate assembly 5 is moved away from the top block, the spring 357 springs back the top block mounting plate 354 to its original position, acting as a stop for the original position of the top block mounting plate.

Further, the utility model is provided with a feeding jacking component in the micropore plate storage bin 12 and the suction plate storage bin 13 respectively, and the feeding jacking component is used for jacking up layer by layer. The micropore plate storage bin 12 and the suction plate storage bin 13 are respectively cylindrical cavities, the micropore plate component 5 is stacked in the inner cavity of the micropore plate storage bin 12, and the suction plate is stacked in the suction plate storage bin 13; the inner cavities of the micro-pore plate storage bin 12 and the suction plate storage bin 13 are respectively provided with a feeding jacking component, the feeding jacking component can realize vertical driving, and when the uppermost micro-pore plate component 5 or suction plate is taken out, the feeding jacking component jacks up one layer so as to be convenient for continuously taking out the micro-pore plate component or suction plate. The jacking height, namely the lifting height of the micro-pore plate assembly 5, can be adjusted by setting parameters, so that the liquid quantity of the sample sucked by the micro-liquid can be adjusted, and all samples can be ensured to be sucked uniformly; through placing the microplate assembly 5 on the sample sucking platform 33, the sample is sucked by the mode that the microplate assembly 5 below rises, so that the working face (namely sucking standard) for sucking the sample is the upper surface of the sample sucking platform 33 when the sample is sucked every time, and the problem of abnormal sample sucking caused by the deviation of the thickness of a trace sample sucking plate can be avoided.

Further, the carrying mechanical arm 21 and the sampling mechanical arm 41 are respectively and slidably mounted on the moving sliding table 6, the moving sliding table 6 is used for driving the carrying mechanical arm 21 and the sampling mechanical arm 41 to move along the X axis, the moving sliding table 6 comprises a guide rail, a driving assembly and two groups of moving platforms, the guide rail and the driving assembly cooperate to drive the two groups of moving platforms, the driving direction is parallel to the driving direction of the linear moving module 31, the carrying mechanical arm 21 and the sampling mechanical arm 41 are respectively mounted on one group of moving platforms, the carrying mechanical arm 21 and the sampling mechanical arm 41 are respectively moved along the X axis, the two groups of moving platforms are respectively arranged on the guide rail, and the two groups of moving platforms do not affect each other in the moving process.

Further, as shown in fig. 18, the sampling mechanical arm 41 includes a Y-axis sampling assembly 411 and a Z-axis sampling assembly 412, where the Y-axis sampling assembly 411 is mounted on the moving sliding table 6 and can move along the X-axis, and the Y-axis sampling assembly 411 is used to drive the Z-axis sampling assembly 412 to move along the Y-axis; the two groups of the Z-axis sample adding assemblies 412 are arranged, each Z-axis sample adding assembly 412 correspondingly drives one sample adding gun 42 to move along the Z axis, the two sample adding guns 42 respectively and independently act, and two liquid drops can be dripped on the micro-pore plate assembly 5 at one time, so that the efficiency of dripping samples is improved. The specific configurations of the Y-axis loading assembly 411 and the Z-axis loading assembly 412 are not limited as long as a linear driving structure can be realized, and will not be described in detail herein.

Further, as shown in fig. 19, the handling robot 21 includes a Y-axis handling unit 211 and a Z-axis handling unit 212, the Y-axis handling unit 211 is mounted on the moving table 6 and can move along the X-axis, and the Y-axis handling unit 211 is used for driving the Z-axis handling unit 212 to move along the Y-axis; jaw 22 is rotatably mounted to Z-axis handling assembly 212, and Z-axis handling assembly 212 is configured to drive jaw 22 to move along the Z-axis, and jaw 22 is rotatable about the Z-axis, and jaw 22 is rotatable to different angles, in coordination with movement in three different directions, XYZ, and jaw 22 moves between microplate storage bin 12, pipetting plate storage bin 13, waste bin 14, support movement platform 32, and the like, thereby transferring the microplate assembly and the pipetting plate.

Still further, the handling robot 21 further includes a pressing plate 213 mounted on the Z-axis handling component 212, the Z-axis handling component 212 drives the pressing plate 213 to move along the Z-axis, when a sample is required to be sucked, the clamping jaw 22 places the sample sucking plate on the sample sucking platform 33, the supporting moving platform 32 is moved to the lower side of the sample sucking platform 33 and stopped by the linear moving module 31, the lifting plate 3324 of the sample sucking lifting component 332 moves upwards to lift the micro-pore plate component 5 placed on the supporting moving platform 32 upwards, the upper surface of the micro-pore plate component 5 contacts with the upper surface of the sample sucking plate, and the upper surface of the sample sucking plate is applied with downward pressure by the pressing plate 213, so that the micro-pore plate component 5 is pressed on the sample sucking plate placed on the sample sucking platform 33, the sample sucking plate is fully sucked to remove the liquid drops on the upper surface of the micro-pore plate component 5, and the sample sucking plate is transferred to the waste bin 14 by the clamping jaw 22 after the sample sucking is completed.

By arranging the parts in the embodiment, the processes of reagent dripping, sample sucking, drying and the like in the pretreatment process can be automatically completed, the automation degree of pretreatment operation is improved, and the harm caused by contact of operators with the reagent can be avoided.

The principles and embodiments of the present application have been described herein with reference to specific examples, the description of which is intended only to facilitate an understanding of the method of the present application and its core ideas. It should be noted that it will be apparent to those skilled in the art that various modifications and adaptations of the application can be made without departing from the principles of the application and these modifications and adaptations are intended to be within the scope of the application as defined in the following claims.

Claims (13)

1. An automated reagent pretreatment system, comprising:

The material storage assembly comprises a suction head placing bin, a micro-pore plate storing bin, a suction plate storing bin, a waste bin and a reagent placing bin;

the moving and carrying assembly comprises a carrying mechanical arm and clamping jaws, the carrying mechanical arm drives the clamping jaws to move, and the clamping jaws can clamp the micro-pore plate assembly from the micro-pore plate storage bin, clamp the suction plate from the suction plate storage bin and transfer the suction plate to the waste bin;

The mobile line assembly comprises a linear mobile module, a supporting mobile platform, a sample sucking platform and a drying platform, wherein the linear mobile module can drive the supporting mobile platform to linearly move, the supporting mobile platform further drives the micro-pore plate assembly to pass through the sample sucking platform and the drying platform, the sample sucking platform sucks samples through a sample sucking plate, and the drying platform heats and dries;

The automatic sample adding assembly comprises a sample adding mechanical arm and a sample adding gun, wherein the sample adding mechanical arm drives the sample adding gun to move, gun suction heads are obtained from the suction head placing bin, and reagents are sucked from the reagent placing bin and added to the micro-pore plate assembly at a sample dropping station in a dropwise manner.

2. The automated reagent pretreatment system of claim 1, wherein the drying deck further comprises:

The drying bracket is arranged on the linear movement module;

a mounting housing secured to the drying rack;

A fan fixed inside the installation housing for blowing an air flow toward the support moving platform;

The heating plate is fixed in the installation shell and is used for heating air flow formed by the fan;

And the temperature fuse is used for high-temperature protection.

3. The automated reagent pretreatment system of claim 1, wherein the sample platform further comprises:

The sample suction bracket is used for placing a sample suction plate;

the sample sucking and jacking assembly is positioned below the sample sucking support and can penetrate through the support moving platform to jack up the micro-pore plate assembly to be in contact with the sample sucking plate.

4. The automated reagent pretreatment system of claim 3, wherein the sample-sucking jacking assembly comprises a jacking motor, a jacking screw rod, a jacking guide rail and a jacking plate, wherein the jacking motor drives the jacking screw rod to rotate, and further drives the jacking plate to vertically move along the jacking guide rail;

wherein, the upper surface of jacking board sets up guide pin and location magnet.

5. The automated reagent pretreatment system of claim 4, wherein the microplate assembly comprises a carrier and a planar microplate, an upper surface of the carrier being provided with a stepped slot for clamping the planar microplate;

The upper surface of the supporting moving platform is provided with a conical guide column for guiding the micro-pore plate assembly.

6. The automatic reagent pretreatment system according to claim 5, wherein the carrier is provided with a fixed magnet, a positioning steel sheet and a guide groove, the positioning magnet can be magnetically matched with the positioning steel sheet, and the guide pin is matched with the guide groove for insertion positioning;

And the supporting mobile platform is provided with a fixed steel sheet matched with the fixed magnet.

7. The automated reagent pretreatment system of claim 6, wherein the carrier is configured to provide a first hand-avoidance position for removal of the planar microplate and a second hand-avoidance position for gripping by the jaws.

8. The automated reagent pretreatment system of claim 1, wherein the mobile line assembly further comprises a secondary positioning assembly for positioning the microplate assembly, the secondary positioning assembly comprising a secondary positioning mounting plate, a linear guide rail, a limiting plate, a top block mounting plate, a top block, a guide screw, and a spring, the linear guide rail being mounted to the secondary positioning mounting plate, the top block mounting plate being translatable along the linear guide rail, the top block being removably mounted to the top block mounting plate; the guide screw is slidably assembled on the limiting plate, and the spring is used for applying elastic force to the top block mounting plate;

The top block is provided with at least two conical blocks which are matched with the guide conical grooves arranged on the micro-pore plate assembly for insertion and positioning.

9. The automated reagent pretreatment system of claim 1, wherein the microplate storage bin and the pipetting plate storage bin are each provided with a feed jacking assembly for jacking up layer by layer.

10. The automated reagent pretreatment system of claim 1, wherein the handling robot and the loading robot are slidably mounted on a moving slide, respectively, the moving slide being configured to drive the handling robot and the loading robot to move along the X-axis.

11. The automated reagent pretreatment system of claim 10, wherein the sampling robot comprises a Y-axis loading assembly and a Z-axis loading assembly, the Y-axis loading assembly configured to drive the Z-axis loading assembly to move along the Y-axis;

The Z-axis sample adding assemblies are arranged in two groups, and each Z-axis sample adding assembly correspondingly drives one sample adding gun to move along the Z axis.

12. The automated reagent pretreatment system of claim 10, wherein the handling robot comprises a Y-axis handling assembly and a Z-axis handling assembly, the Y-axis handling assembly configured to drive the Z-axis handling assembly to move along a Y-axis;

The clamping jaw is rotatably installed in the Z-axis carrying assembly, and the Z-axis carrying assembly is used for driving the clamping jaw to move along the Z axis and can rotate around the Z axis.

13. The automated reagent pretreatment system of claim 12, wherein the handling robot further comprises a platen mounted to the Z-axis handling assembly, the Z-axis handling assembly driving the platen to move along the Z-axis to press against a sample plate placed on the sample platform.