CN221274048U - PCR kit for detecting avian infectious bronchitis virus - Google Patents
PCR kit for detecting avian infectious bronchitis virus Download PDFInfo
- Publication number
- CN221274048U CN221274048U CN202323454567.XU CN202323454567U CN221274048U CN 221274048 U CN221274048 U CN 221274048U CN 202323454567 U CN202323454567 U CN 202323454567U CN 221274048 U CN221274048 U CN 221274048U
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- China
- Prior art keywords
- kit
- fixedly arranged
- refrigerating
- infectious bronchitis
- avian infectious
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- 241000711450 Infectious bronchitis virus Species 0.000 title claims abstract description 20
- 238000009004 PCR Kit Methods 0.000 title claims description 18
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 19
- 238000005057 refrigeration Methods 0.000 claims abstract description 17
- 230000007246 mechanism Effects 0.000 claims abstract description 8
- 238000005070 sampling Methods 0.000 claims abstract description 6
- 229920001495 poly(sodium acrylate) polymer Polymers 0.000 claims description 9
- NNMHYFLPFNGQFZ-UHFFFAOYSA-M sodium polyacrylate Chemical compound [Na+].[O-]C(=O)C=C NNMHYFLPFNGQFZ-UHFFFAOYSA-M 0.000 claims description 9
- 238000004321 preservation Methods 0.000 claims description 8
- 238000001514 detection method Methods 0.000 abstract description 6
- 238000003752 polymerase chain reaction Methods 0.000 abstract description 6
- 206010011409 Cross infection Diseases 0.000 abstract description 4
- 206010029803 Nosocomial infection Diseases 0.000 abstract description 4
- 238000000034 method Methods 0.000 description 5
- 159000000000 sodium salts Chemical group 0.000 description 5
- 238000007689 inspection Methods 0.000 description 4
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 3
- 230000009471 action Effects 0.000 description 3
- 238000006243 chemical reaction Methods 0.000 description 2
- 239000003153 chemical reaction reagent Substances 0.000 description 2
- 238000009413 insulation Methods 0.000 description 2
- 230000008569 process Effects 0.000 description 2
- 239000000243 solution Substances 0.000 description 2
- 230000004075 alteration Effects 0.000 description 1
- 208000022362 bacterial infectious disease Diseases 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 210000004556 brain Anatomy 0.000 description 1
- 238000001816 cooling Methods 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 210000003608 fece Anatomy 0.000 description 1
- 210000004072 lung Anatomy 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 210000004165 myocardium Anatomy 0.000 description 1
- 238000004806 packaging method and process Methods 0.000 description 1
- 210000002976 pectoralis muscle Anatomy 0.000 description 1
- 239000002504 physiological saline solution Substances 0.000 description 1
- 230000002035 prolonged effect Effects 0.000 description 1
- 210000002345 respiratory system Anatomy 0.000 description 1
- 230000028327 secretion Effects 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
Landscapes
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
The utility model discloses a PCR (polymerase chain reaction) kit for detecting avian infectious bronchitis virus, which comprises a kit, wherein a mounting frame is fixedly arranged in the kit, a plurality of storage tanks are uniformly and fixedly arranged in the mounting frame, a storage pipe is arranged in the storage tank, a refrigerating mechanism for keeping sampling temperature is fixedly arranged at the lower end of the kit, the refrigerating mechanism comprises a refrigerating bin, a through groove, a water bag, a screw rod, a thimble and a storage bag, the lower end of the kit is fixedly provided with the refrigerating bin, the through groove is arranged between the upper end of the inside of the refrigerating bin and the kit, and the water bag is fixedly arranged at the lower end of the inside of the design box. The utility model can automatically refrigerate after each sampling is completed; the refrigerating operation is convenient and the cost is low; is disposable, and is not reused after the use is completed; compared with the traditional refrigeration equipment, the cross infection is not worried about; thereby ensuring the accuracy of detection.
Description
Technical Field
The utility model relates to the technical field of PCR (polymerase chain reaction) kits, in particular to a PCR kit for detecting avian infectious bronchitis viruses.
Background
The PCR detection method has extremely important significance in the aspects of rapidly diagnosing bacterial infectious diseases clinically and the like.
In the prior art, the detection of avian infectious bronchitis viruses is also commonly performed by a PCR detection method; the basic operation is as follows; collecting sample to kill or kill fowl, taking laryngotracheae, brain, pectoral muscle, cardiac muscle and lung, taking respiratory tract secretion or excreta from the fowl to be detected, storing in 50% glycerol physiological saline at about 8 deg.C, and laboratory detecting.
In the prior art, the sample is required to be stored at a low temperature after being collected; the PCR kit in the prior art has single function in use and can only play a simple packaging function; the special heat preservation equipment or the special ice box is required to be additionally used for heat preservation operation every time when heat preservation is required, and the ice box or the heat preservation equipment is required to be disinfected every time in order to prevent cross infection every time; there is therefore a need for an improved PCR kit for detecting avian infectious bronchitis virus.
Disclosure of utility model
The utility model aims to provide a PCR kit for detecting avian infectious bronchitis virus, so as to solve the problems in the background art.
In order to achieve the above purpose, the present utility model provides the following technical solutions: the PCR kit for detecting the avian infectious bronchitis virus comprises a kit, wherein a mounting frame is fixedly arranged in the kit, a plurality of storage tanks are uniformly and fixedly arranged in the mounting frame, a storage pipe is arranged in the storage tank, a refrigerating mechanism for keeping sampling temperature is fixedly arranged at the lower end of the kit, the refrigerating mechanism comprises a refrigerating bin, a through tank, a water bag, a screw rod, a thimble and a storage bag, the lower end of the kit is fixedly provided with the refrigerating bin, the through tank is arranged between the upper end of the inside of the refrigerating bin and the kit, and the water bag is fixedly arranged at the lower end of the inside of the design box; the lower end of the inside of the refrigerating bin is provided with a screw through movable screwing of threads, the upper end of the screw is fixedly provided with a thimble, and both sides of the inside of the refrigerating bin are fixedly provided with storage bags.
Preferably, the screw lower extreme is fixedly provided with twists the movable block, can conveniently rotate the screw rod through twisting the movable block.
Preferably, a horn mouth is arranged in the middle of the lower end of the refrigeration bin; the finger of a user can be conveniently inserted into the horn mouth and then the screw rod is rotated.
Preferably, a top cover is fixedly arranged on the outer side surface of the upper end of the kit; the top cover can be fixed at the upper end of the kit in an interference connection mode; or can be fixed by a buckle and the like.
Preferably, sodium polyacrylate is stored in the storage bag, small holes are formed in the outer side surface of the storage bag, and the sodium polyacrylate is sodium salt and can generate chemical reaction when contacting with water; so that heat can be absorbed to lower the temperature of the whole device.
Preferably, the outer side surface of the kit is provided with a thermal insulation sleeve.
Compared with the prior art, the utility model has the beneficial effects that:
1. The utility model can cool down autonomously; meanwhile, the device is not reused after disposable use is completed; compared with the traditional refrigeration equipment, the cross infection is not worried about; thereby ensuring the accuracy of detection.
2. The utility model has convenient refrigeration operation, and can automatically perform refrigeration only by rotating the screw rod to puncture the ice bag; the method is simple and safe without any operation difficulty; meanwhile, the device has extremely low refrigeration cost, and the sodium polyacrylate and water contact refrigeration is a common refrigeration means of a disposable ice bag in the prior art; can be mass-produced; is convenient for popularization and use.
Drawings
FIG. 1 is a schematic diagram of the overall structure of a PCR kit for detecting avian infectious bronchitis virus according to the present utility model;
FIG. 2 is a bottom view of a PCR kit for detecting avian infectious bronchitis virus according to the present utility model;
FIG. 3 is a reservoir mounting view of a PCR kit for detecting avian infectious bronchitis virus according to the present utility model;
FIG. 4 is a cross-sectional view of a kit of the PCR kit for detecting avian infectious bronchitis virus according to the present utility model.
In the figure: 1. a kit; 2. a mounting frame; 3. a storage tank; 4. a storage tube; 5. a refrigerating bin; 6. a through groove; 7. a water bag; 8. a screw; 9. a thimble; 10. a storage bag; 11. twisting the block; 12. a horn mouth; 13. and a top cover.
Detailed Description
The following description of the embodiments of the present utility model will be made clearly and completely with reference to the accompanying drawings, in which it is apparent that the embodiments described are only some embodiments of the present utility model, but not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the utility model without making any inventive effort, are intended to be within the scope of the utility model.
Referring to fig. 1-4, the present utility model provides a technical solution: the PCR kit 1 for detecting avian infectious bronchitis viruses comprises a kit 1, wherein a mounting frame 2 is fixedly arranged inside the kit 1, a plurality of storage tanks 3 are uniformly and fixedly arranged inside the mounting frame 2, a storage pipe 4 is arranged inside the storage tanks 3, a refrigerating mechanism for keeping sampling temperature is fixedly arranged at the lower end of the kit 1, the refrigerating mechanism comprises a refrigerating bin 5, a through groove 6, a water bag 7, a screw rod 8, a thimble 9 and a storage bag 10, the refrigerating bin 5 is fixedly arranged at the lower end of the kit 1, a through groove 6 is arranged between the upper end inside the refrigerating bin 5 and the kit 1, and a water bag 7 is fixedly arranged at the lower end inside the design box; the lower end of the inside of the refrigerating bin 5 is provided with a screw rod 8 through movable screwing of threads, the upper end of the screw rod 8 is fixedly provided with a thimble 9, and both sides of the inside of the refrigerating bin 5 are fixedly provided with storage bags 10.
The screw rod 8 is fixedly provided with a screwing block 11 at the lower end, and the screw rod 8 can be conveniently rotated by screwing the block 11;
The middle part of the lower end of the refrigeration bin 5 is provided with a horn mouth 12; the finger of a user can be conveniently inserted into the horn mouth 12 and then the screw rod 8 is rotated;
A top cover 13 is fixedly arranged on the outer side surface of the upper end of the kit 1; the top cover 13 can be fixed at the upper end of the kit 1 in an interference connection manner; the fixing can also be realized by means of a buckle and the like;
Sodium polyacrylate is stored in the storage bag 10, small holes are formed in the outer side surface of the storage bag 10, and the sodium polyacrylate is sodium salt and can generate chemical reaction when contacting with water; thereby absorbing heat to reduce the temperature of the whole device;
The outer side surface of the kit 1 is provided with a heat preservation sleeve; the device utilizes the sodium salt and water refrigeration principle; the continuous refrigeration time is not very long; insulation sleeves are used for isolating heat exchange; so that the device can maintain stability below 10 ℃ for about two hours and three hours; this time is sufficient for inspection; if the refrigerating time is insufficient, a kit 1 can be prepared; the sodium salt and water refrigeration cost is extremely low; the design box can be recycled and melted for producing other materials; so that the use cost is not increased too much; or the reagent kit 1 and the refrigeration bin 5 can be made into a double-layer sandwich type according to the requirement; the heat preservation effect can also be improved.
Working principle: when the device is used, the kit 1 of the device can store the storage tube 4 by utilizing the storage tank 3; and a sampling sample is stored in the storage tube 4; storing a sample and a reagent for detecting avian infectious bronchitis virus; the inspection can be performed; the storage tube 4 should be stored at low temperature during inspection; typically about 2 to 10 degrees; at this time, the device does not need to be put into an ice chest or a refrigerating device; the screw rod 8 can be rotated through threads, so that the screw rod 8 moves upwards, and the water bag 7 can be pierced through the thimble 9; so that the water in the water bag 7 enters the refrigerating bin 5 through the through groove 6; and is brought into contact with sodium salt (sodium polyacrylate) inside the storage bag 10 through the storage bag 10; the sodium polyacrylate will react chemically when in contact with water; so that the heat can be absorbed to reduce the temperature of the whole device, and the contact refrigeration of sodium polyacrylate and water is also a common refrigeration means of the disposable ice bag in the prior art; the cooling time can be prolonged by matching the shell of the kit 1 of the device and the heat preservation layer on the outer side; so that the device has enough time for inspection; the device can automatically cool down, and the refrigerating operation is convenient and the cost is low; meanwhile, the device is not reused after disposable use is completed; compared with the traditional repeated refrigeration equipment, the cross infection is not worried about; thereby ensuring the accuracy of detection.
It is noted that relational terms such as first and second, and the like are used solely to distinguish one entity or action from another entity or action without necessarily requiring or implying any actual such relationship or order between such entities or actions. Moreover, the terms "comprises," "comprising," or any other variation thereof, are intended to cover a non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements does not include only those elements but may include other elements not expressly listed or inherent to such process, method, article, or apparatus.
Although embodiments of the present utility model have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made therein without departing from the principles and spirit of the utility model, the scope of which is defined in the appended claims and their equivalents.
Claims (6)
1. A PCR kit for detecting avian infectious bronchitis virus, comprising a kit (1), characterized in that: the kit comprises a kit body, and is characterized in that a mounting frame (2) is fixedly arranged inside the kit body, a plurality of storage tanks (3) are uniformly and fixedly arranged inside the mounting frame (2), a storage pipe (4) is arranged inside the storage tanks (3), a refrigerating mechanism for keeping sampling temperature is fixedly arranged at the lower end of the kit body, the refrigerating mechanism comprises a refrigerating bin (5), a through groove (6), a water bag (7), a screw (8), a thimble (9) and a storage bag (10), the refrigerating bin (5) is fixedly arranged at the lower end of the kit body, a through groove (6) is formed between the upper end inside the refrigerating bin (5) and the kit body (1), and the water bag (7) is fixedly arranged at the lower end inside the kit body; the inner lower end of the refrigerating bin (5) is movably screwed with a screw (8) through threads, a thimble (9) is fixedly arranged at the upper end of the screw (8), and storage bags (10) are fixedly arranged on two sides of the inner part of the refrigerating bin (5).
2. The PCR kit for detecting avian infectious bronchitis virus according to claim 1, wherein: the lower end of the screw rod (8) is fixedly provided with a screwing block (11).
3. The PCR kit for detecting avian infectious bronchitis virus according to claim 1, wherein: the middle part of the lower end of the refrigeration bin (5) is provided with a horn mouth (12).
4. The PCR kit for detecting avian infectious bronchitis virus according to claim 1, wherein: the surface of the outer side of the upper end of the kit (1) is fixedly provided with a top cover (13).
5. The PCR kit for detecting avian infectious bronchitis virus according to claim 1, wherein: sodium polyacrylate is stored in the storage bag (10), and small holes are formed in the outer side surface of the storage bag (10).
6. The PCR kit for detecting avian infectious bronchitis virus according to claim 1, wherein: the outer side surface of the kit (1) is provided with a heat preservation sleeve.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202323454567.XU CN221274048U (en) | 2023-12-19 | 2023-12-19 | PCR kit for detecting avian infectious bronchitis virus |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202323454567.XU CN221274048U (en) | 2023-12-19 | 2023-12-19 | PCR kit for detecting avian infectious bronchitis virus |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN221274048U true CN221274048U (en) | 2024-07-05 |
Family
ID=91705957
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202323454567.XU Active CN221274048U (en) | 2023-12-19 | 2023-12-19 | PCR kit for detecting avian infectious bronchitis virus |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN221274048U (en) |
-
2023
- 2023-12-19 CN CN202323454567.XU patent/CN221274048U/en active Active
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