CN220677876U - Automatic change micro-fluidic chip leak protection liquid priming device - Google Patents

Abstract

The utility model discloses an automatic microfluidic chip leakage-proof liquid injection device, which relates to the technical field related to microfluidic chips and comprises an injection mechanism and a driving mechanism; the injection mechanism comprises a liquid inlet pipe, an inner sealing ring, a pipe clamping sleeve, a connecting seat, an inner sealing ring and the like; the driving mechanism is used for controlling the injection mechanism to move and rotate so that the pipe clamping sleeve is rotationally inserted into and removed from the guide channel of the connecting seat in a threaded connection mode, and the pipe clamping sleeve in an inserted state is elastically clamped between the seat inner sealing ring and the seat inner sealing ring; therefore, when the liquid leakage prevention device is applied, the pipe clamping sleeve and the guide channel can be in threaded connection to realize the position positioning of each sealing ring, so that the sealing rings in the sleeve, the pipe clamping sleeve and the sealing rings in the seat can be kept in tight contact, the liquid leakage condition is avoided, and the problem that liquid is difficult to leak out in the prior art is practically solved.

Description

Automatic change micro-fluidic chip leak protection liquid priming device

Technical Field

The utility model relates to the technical field related to microfluidic chips, in particular to an automatic microfluidic chip leakage-proof liquid injection device.

Background

The microfluidic chip technology integrates basic operation units of sample preparation, reaction, separation, detection and the like in chemical, biological and medical analysis processes on a micron-scale chip, and automatically completes the whole analysis process, and the technology has been widely researched and applied in the fields of biology, chemistry, medicine and the like.

At present, a microfluidic chip is quite common in a microfluidic laboratory, however, the microfluidic chip still has a technical pain point, namely the problem of liquid leakage at the joint between the chip and a liquid inlet pipe, and the problem of liquid leakage at the liquid inlet usually occurs due to the fact that a pipeline at the joint cannot be well matched with the liquid inlet, and the pipeline of the microfluidic chip has higher hydraulic pressure when cell sample liquid is injected. The measure about the leakage prevention of the microfluidic chip is to coat AB glue on the joint of the liquid inlet and the liquid inlet pipe of the microfluidic chip for sealing, but the leakage prevention cannot be realized because the AB glue cannot continuously fill the gap between the pipeline and the hole when the liquid pressure of the injection is high due to the characteristic of the AB glue, and the leakage prevention method is disposable if the AB glue is used for realizing the leakage prevention, so that unnecessary waste is caused. If the microfluidic chip cannot realize liquid leakage prevention, a large amount of sample liquid is wasted, and the advantage of small demand for the sample liquid of the microfluidic chip cannot be reflected.

Therefore, developing a technical scheme capable of realizing liquid inlet leakage prevention has become a technical problem to be solved urgently.

Disclosure of Invention

The utility model aims to provide an automatic microfluidic chip leakage-proof liquid injection device so as to solve the problem that liquid is difficult to leak in the prior art.

In order to solve the technical problems, the utility model provides an automatic microfluidic chip leakage-proof liquid injection device, which comprises an injection mechanism and a driving mechanism; the injection mechanism comprises a liquid inlet pipe, an inner sleeve sealing ring, a pipe clamping sleeve, a connecting seat and an inner seat sealing ring; the liquid inlet pipe penetrates through the pipe clamping sleeve; the sleeve inner sealing ring is sleeved outside the liquid inlet pipe, the sleeve inner sealing ring is arranged at one end of the pipe clamping sleeve for insertion operation, and the sleeve inner sealing ring is elastically clamped between the pipe clamping sleeve and the liquid inlet pipe; the outer peripheral wall of the pipe clamping sleeve is provided with external threads; the connecting seat is provided with a guide channel which penetrates through the connecting seat, and the inner peripheral wall of the guide channel is provided with internal threads; the seat inner sealing ring is arranged in the axial direction of the guide channel, and is arranged adjacent to the outlet of the guide channel; the driving mechanism is used for controlling the injection mechanism to move and rotate so that the pipe clamping sleeve is rotationally inserted into and removed from the guide channel in a threaded connection mode, and the inserted state of the pipe clamping sleeve is elastically clamped between the seat inner sealing ring and the sleeve inner sealing ring.

In one embodiment, the guide channel comprises a thread section and a narrowing section which are communicated with each other, the inner peripheral wall of the thread section is provided with the internal thread, and the inner diameter of the narrowing section changes in a narrowing way in the direction from the thread section to the seat inner sealing ring; the clamping pipe sleeve comprises a connecting section and a clamping section which are mutually communicated; the outer peripheral wall of the connecting section is provided with the external thread; the inner diameter of the clamping section is narrowed and changed along the direction that the clamping sleeve is inserted into the guide channel, and the inner sealing ring in the sleeve is elastically clamped in the clamping section; and the narrowing of the narrowing is greater than the narrowing of the clamping section.

In one embodiment, the inner diameter of the seat inner seal varies narrowly in a direction from the guide channel toward the seat inner seal.

In one embodiment, the seat inner sealing ring comprises a reducing section and a constant diameter section which are communicated with each other; the diameter-variable section is communicated with the guide channel, and the inner diameter of the diameter-variable section is narrowed and changed in the direction from the guide channel to the constant diameter section; the inner diameter of the constant diameter section is constant.

In one embodiment, the sleeve inner sealing ring comprises a thick-wall section and a thin-wall section which are communicated with each other; the wall thickness of the thick-wall section is larger than that of the thin-wall section, and the thick-wall section is elastically clamped between the pipe clamping sleeve and the liquid inlet pipe; the thin-wall section extends out of the port of the clip tube sleeve for insertion operation.

In one embodiment, the clamping section is provided with at least one sleeve through groove, and the sleeve through groove extends between two ports of the clamping section.

In one embodiment, the drive mechanism includes a moving unit and a rotating unit; the moving unit is used for driving the rotating unit and the injection mechanism to synchronously translate and lift; the rotating unit clamps the peripheral wall of the pipe clamping sleeve in an interference fit mode, and is used for controlling the pipe clamping sleeve to rotate.

In one embodiment, the rotary unit includes a rotary motor, a transmission gear, and a flat key; the rotating motor is used for controlling the rotation of the transmission gear by utilizing gear transmission; the transmission gear is sleeved outside the pipe clamping sleeve, and a mounting groove is formed in the inner wall, facing the pipe clamping sleeve, of the transmission gear; the flat key is arranged in the mounting groove and clamped between the transmission gear and the pipe clamping sleeve.

In one embodiment, the device further comprises a clamping mechanism, wherein the clamping mechanism comprises clamping blocks, guide posts and air cylinders, the two clamping blocks are respectively arranged on two opposite sides of the connecting seat, the two clamping blocks are respectively inserted into the connecting seat by utilizing the guide posts in a sliding mode, two telescopic arms of the two air cylinders are respectively connected with the two clamping blocks, and the air cylinders are used for driving the two clamping blocks to move oppositely and separately.

The beneficial effects of the utility model are as follows:

when the infusion device is used, the driving mechanism is used for controlling the injection mechanism to move and rotate, so that the pipe clamping sleeve is rotationally inserted into and removed from the guide channel in a threaded connection mode, and the pipe clamping sleeve in an inserted state is elastically clamped between the seat inner sealing ring and the seat inner sealing ring, so that after the infusion is carried out on the liquid inlet pipe, the pipe clamping sleeve and the guide channel can realize the position positioning of each sealing ring by using the threaded connection, the inner sealing ring, the pipe clamping sleeve and the seat inner sealing ring can be kept in tight abutting connection, the occurrence of liquid leakage is avoided, and the problem that liquid inlet is difficult to leak prevention in the prior art is practically solved.

Drawings

In order to more clearly illustrate the technical solutions of the present utility model, the drawings that are needed in the embodiments will be briefly described below, and it is obvious that the drawings in the following description are only some embodiments of the present utility model, and that other drawings can be obtained according to these drawings without inventive effort for a person skilled in the art.

FIG. 1 is a schematic diagram of a structure provided by an embodiment of the present utility model;

FIG. 2 is a schematic view of the partially disassembled structure of FIG. 1;

FIG. 3 is a schematic view of the partially enlarged structure of FIG. 2;

FIG. 4 is a schematic illustration of the clamping structure of FIG. 1 in a disassembled configuration;

FIG. 5 is a schematic cross-sectional view of the application of FIG. 1;

FIG. 6 is an enlarged schematic view of the structure of FIG. 5;

fig. 7 is a schematic view of the jacket sleeve structure of fig. 1.

The reference numerals are as follows:

10. an injection mechanism; 11. a liquid inlet pipe; 12. a sealing ring is sleeved in the sleeve; 121. a thick-walled segment; 122. a thin wall section; 13. clamping the pipe sleeve; 131. an external thread; 132. a connection section; 133. a clamping section; 134. sleeving a through groove; 14. a connecting seat; 141. a guide channel; 142. an internal thread; 143. a threaded section; 144. a narrowing section; 15. a seal ring in the seat; 151. a reducing section; 152. a constant diameter section;

20. a driving mechanism; 21. a mobile unit; 22. a rotating unit; 221. a rotating electric machine; 222. a transmission gear; 223. a flat key; 224. a power gear;

30. a clamping mechanism; 31. clamping blocks; 32. a cylinder; 33. a guide post;

40. and a microfluidic chip.

Detailed Description

The technical solutions in the embodiments of the present utility model will be clearly and completely described below with reference to the accompanying drawings in the embodiments of the present utility model.

The utility model provides an automatic microfluidic chip liquid leakage prevention liquid injection device, which is implemented as shown in fig. 1 to 7 and comprises an injection mechanism 10 and a driving mechanism 20.

Regarding the injection mechanism 10, the main function of the injection mechanism is to perform the injection operation on the microfluidic chip 40, and in order to ensure good leakage-proof performance during the injection operation, the injection mechanism 10 includes a liquid inlet pipe 11, an inner sealing ring 12, a clamping sleeve 13, a connecting seat 14, and an inner sealing ring 15.

As shown in fig. 5, the liquid inlet pipe 11 is in a cylindrical long pipe shape, the inside of the liquid inlet pipe is hollow to realize liquid infusion, when the liquid inlet pipe 11 is installed, the liquid inlet pipe 11 penetrates through the pipe clamping sleeve 13, and the liquid outlet end of the liquid inlet pipe 11 penetrates out of the pipe clamping sleeve 13, so that the liquid outlet end of the liquid inlet pipe 11 penetrates through each sealing ring and then is communicated with the liquid inlet end of the microfluidic chip 40; the liquid outlet end of the liquid inlet pipe 11 is in a bevel structure, so that the liquid inlet pipe 11 can better penetrate through each sealing ring.

As shown in fig. 5, the overall shape of the inner seal ring 12 is substantially tubular, the inner seal ring 12 is sleeved outside the liquid inlet pipe 11, the inner seal ring 12 is arranged at one end of the pipe clamping sleeve 13 for insertion operation, and the inner seal ring 12 is elastically clamped between the pipe clamping sleeve 13 and the liquid inlet pipe 11; for example, in the illustrated direction, the clip tube housing 13 is inserted by its lower end, so that the in-housing seal ring 12 is placed in the space surrounded by the lower end of the clip tube housing 13, and the in-housing seal ring 12 pushes the lower end of the clip tube housing 13 to expand outwards without being affected by other external forces.

As shown in fig. 5, the jacket sleeve 13 is generally long tubular in shape, and hollow in the inside thereof so that the liquid inlet pipe 11 is inserted therein, and the outer peripheral wall of the jacket sleeve 13 is provided with external threads 131, and the external threads 131 are arranged in a sufficient range extending in the length direction of the jacket sleeve 13.

As shown in fig. 4 and 5, the upper part of the connecting seat 14 is cylindrical, the lower part is rectangular, the connecting seat 14 is provided with a guide channel 141 penetrating the connecting seat, and the inner peripheral wall of the guide channel is provided with an internal thread 142; specifically, in the illustrated orientation, the guide channel 141 extends through the connecting seat 14 from top to bottom, and the internal thread 142 is disposed in the cylindrical structure of the connecting seat 14.

As shown in fig. 4 and 5, the overall shape of the in-seat seal ring 15 is substantially pipe-shaped and annular, the in-seat seal ring 15 is provided in the axial direction of the guide passage 141, and the in-seat seal ring 15 is disposed adjacent to the outlet of the guide passage 141; specifically, in the direction shown in the drawing, the lower end of the guide channel 141 is its own outlet, and the lower portion of the connecting seat 14 is provided with a corresponding groove, so that the seal ring 15 in the seat can be disposed in the groove to achieve fixation.

And the driving mechanism 20 is mainly used for controlling the movement and rotation of the injection mechanism 10 so that the pipe clamping sleeve 13 is rotationally inserted into and removed from the guide channel 141 in a threaded connection manner, and the pipe clamping sleeve 13 in an inserted state is elastically clamped between the seat inner sealing ring 15 and the seat inner sealing ring 12.

As shown in fig. 1 and 5, in order to realize automatic alignment of the liquid inlet tube 11 and the microfluidic chip 40, the driving mechanism 20 is provided in this embodiment and includes a moving unit 21 and a rotating unit 22; the moving unit 21 is used for driving the rotating unit 22 and the injection mechanism 10 to synchronously translate and lift; the rotating unit 22 clamps the outer peripheral wall of the pipe clamping sleeve 13 in an interference fit mode, and the rotating unit 22 is used for controlling the pipe clamping sleeve 13 to rotate.

As shown in fig. 1, the moving unit 21 is configured to move the rotating unit 22 and the injection mechanism 10 forward, backward, leftward, rightward, upward, and downward, and is driven by a screw drive in three directions XYZ.

As shown in fig. 1 to 3, the above-mentioned rotating unit 22, the rotating unit 22 at this time includes a rotating motor 221, a transmission gear 222, and a flat key 223; the rotary motor 221 is used for controlling the rotation of the transmission gear 222 by gear transmission; the transmission gear 222 is sleeved outside the pipe clamping sleeve 13, and the inner wall of the transmission gear 222 facing the pipe clamping sleeve 13 is provided with a mounting groove; the flat key 223 is arranged in the mounting groove, and the flat key 223 is clamped between the transmission gear 222 and the pipe clamping sleeve 13.

Specifically, at this time, the output shaft of the rotating motor 221 is horizontally arranged and is provided with a power gear 224 coaxially arranged with the output shaft, and the transmission gear 222 is meshed with the power gear 224 in an axially vertical arrangement manner, so that power transmission and rotation direction conversion between the power gear 224 and the transmission gear 222 are realized; of course, the gear transmission mode is not limited to this, and a gear set or other compound transmission structure including gear transmission can be selectively arranged according to the requirement.

The transmission gear 222 of this embodiment penetrates along the axial direction thereof, so that the transmission gear 222 can be sleeved outside the pipe clamping sleeve 13, and at this time, the interference fit between the rotation unit 22 and the pipe clamping sleeve 13 is mainly realized by using the flat key 223; after selecting the flat key 223 with the size and specification meeting the requirements, the flat key 223 can ensure that the pipe clamping sleeve 13 can be stably clamped in the transmission gear 222, and as the main clamping force is generated by the contact between the flat key 223 and the pipe clamping sleeve 13, once the pipe clamping sleeve 13 starts to be in threaded connection with the guide conduction, the acting force generated by the threaded connection can also cause the pipe clamping sleeve 13 to overcome the original clamping force between the flat key 223 so as to realize the up-and-down movement of the pipe clamping sleeve 13, namely the threaded connection control between the pipe clamping sleeve 13 and the guide channel 141 is realized.

It should be further noted that, to ensure that the liquid inlet tube 11 can accurately infuse the microfluidic chip 40, it should generally be ensured that the microfluidic chip 40 and the liquid inlet tube 11 can be kept in a relatively stable state, and other related clamping structures may be used to clamp the microfluidic chip 40.

The embodiment realizes the integration of the clamping function, as shown in fig. 1, 2, 4 and 5, the clamping mechanism 30 for clamping and fixing the microfluidic chip 40 is arranged on the connecting seat 14, the clamping mechanism 30 comprises clamping blocks 31 and air cylinders 32, anti-slip patterns are arranged on the clamping surfaces of the two clamping blocks 31, the two clamping blocks 31 are respectively arranged on two opposite sides of the connecting seat 14, the two clamping blocks 31 are respectively inserted into the connecting seat 14 in a sliding manner by using guide posts 33, and two telescopic arms of the air cylinders 32 are respectively connected with the two clamping blocks 31, so that the two clamping blocks 31 are driven to move towards and away from each other, namely, the clamping of the microfluidic chip 40 is realized by using the opposite movement, and the fixing of the microfluidic chip 40 is released by using the opposite movement, so that the stable alignment transfusion of the microfluidic chip 40 and the injection mechanism 10 is realized.

The main structural principles of the components of this embodiment are known from the foregoing, and for a better understanding of the application principles of the present utility model, the following description will be provided in connection with specific application procedures, specifically including the following operation steps:

in the first step, when it is detected by infrared ranging that the connection pad 14 is in contact with the microfluidic chip 40, the movement of the moving unit 21 is stopped. If the priming port of the priming device does not successfully enter the priming port of the microfluidic chip 40, the mobile unit 21 immediately stops operating and gives an alarm to prevent the chip and priming device from being damaged. If the docking is successful, the driving jaws clamp the microfluidic chip 40.

In the second step, after the priming device is assembled and fixed to the microfluidic chip 40, the rotation unit 22 starts to automatically operate, so as to drive the pipe clamping sleeve 13 to rotate, the threads of the pipe clamping sleeve 13 contact with the threads of the connecting seat 14 and start to be screwed, and when the screwing degree is higher, the sealing degree is stronger, so that the effect of adjusting the sealing degree is achieved.

And thirdly, screwing the pipe clamping sleeve 13 into the connecting seat 14, and sealing the inner sealing ring 12 of the clamping sleeve at the lower end of the pipe clamping sleeve 13 to achieve the sealing between the liquid inlet pipe 11 and the pipe clamping sleeve 13 by utilizing the different taper of the contact surface of the connecting seat 14 and the pipe clamping sleeve 13. The outer surface of the lower end of the pipe clamping sleeve 13 is contacted and extruded with the seat inner sealing ring 15 to achieve the sealing state of the pipe clamping sleeve 13 and the microfluidic chip 40, so that interference fit is formed. The system is annotated liquid and will be adopted branch gradient and annotate liquid, in order to prevent that the degree of screwing is too high, leads to pressing from both sides the life decline of pipe box 13, and the system adjusts the degree of screwing and can reach a fixed gradient earlier to control the syringe pump simultaneously and increase the annotate liquid power, when detecting that annotate liquid pressure is too big, there is the weeping risk, the system automatically increases the degree of screwing of pressing from both sides pipe box 13 and connecting seat 14, reaches the second gradient. In order not to damage the jacket sleeve 13, a total of 3 gradients are provided. When the third gradient cannot bear the condition that liquid leakage occurs, the operation of the injection pump can be automatically stopped, liquid injection is stopped, the injection device enters a protection locking state, the combination state of the liquid injection device and the micro-fluidic chip 40 is maintained, and the locking state can not be released until the pressure of the inner cavity at the joint of the liquid injection port is reduced.

Fourth, after the system detects that the liquid injection is finished, the system can reserve a period of time and then perform a disassembling and washing process after the liquid pressure in the liquid injection port is reduced, so that the sample liquid is prevented from leaking to pollute the environment when the device is lifted. The rotary unit 22 then automatically rotates in the reverse direction to unscrew the jacket sleeve 13, and stops pressing the sleeve inner seal ring 12 and the seat inner seal ring 15. The clamping mechanism 30 automatically stops clamping the chip, and when the chip is detected not to be in contact with the surface of the clamping piece any more, the moving unit 21 starts to operate, the whole liquid injection device is separated from the chip, and the liquid leakage prevention device can be quickly disassembled, and the chip and the liquid injection device are guaranteed to be completely separated.

In order to enhance the sealing and leakage preventing performance, as shown in fig. 5, the guiding channel 141 is provided with a thread section 143 and a narrowing section 144 which are mutually communicated, the inner peripheral wall of the thread section 143 is provided with an internal thread 142, and the inner diameter of the narrowing section 144 changes narrowly in the direction from the thread section 143 to the seat inner sealing ring 15; the clamping sleeve 13 comprises a connecting section 132 and a clamping section 133 which are communicated with each other; the outer peripheral wall of the connecting section 132 is provided with external threads 131; the inner diameter of the clamping section 133 changes narrowly along the direction of inserting the clamping sleeve 13 into the guide channel 141, and the inner sealing ring 12 in the sleeve is elastically clamped in the clamping section 133; and the narrowing of the narrowing section 144 is greater than the narrowing of the clamping section 133.

In application, since the narrowing width of the narrowing section 144 is larger than that of the clamping section 133, as the pipe clamping sleeve 13 continuously goes deep into the guide channel 141, the narrowing section 144 will contact with the clamping section 133 and gradually strengthen the inward pushing of the clamping section 133, so that the clamping force of the clamping section 133 to the sealing ring 12 in the sleeve is enhanced, and the purpose of enhancing the sealing and leakage preventing performance is achieved.

As shown in fig. 5 and 6, in this embodiment, the inside diameter of the seat inner seal 15 changes narrowly in the direction from the guide passage 141 toward the seat inner seal 15.

After the arrangement mode is adopted, when the depth of the pipe clamping sleeve 13 is deeper, the clamping force between the seat inner sealing ring 15 and the sleeve inner sealing ring 12 is stronger, so that the tightness can be gradually improved; when the pipe clamping sleeve 13 starts to separate, the clamping force between the seat inner sealing ring 15 and the sleeve inner sealing ring 12 is weakened, namely the pipe clamping sleeve 13 is also convenient to separate.

As shown in fig. 5 and 6, the seal ring 15 in the seat of this embodiment includes a reducing section 151 and a constant diameter section 152 which are connected to each other; the variable diameter section 151 is communicated with the guide channel 141, and the inner diameter of the variable diameter section 151 is narrowed and changed in the direction from the guide channel 141 to the constant diameter section 152; the constant diameter section 152 has a constant inner diameter.

After the arrangement, the constant diameter section 152 can be used to increase the contact area, so as to further improve the sealing effect.

As shown in fig. 5 and 6, the sleeve inner seal ring 12 according to this embodiment includes a thick-wall section 121 and a thin-wall section 122 that are connected to each other; the wall thickness of the thick-wall section 121 is larger than that of the thin-wall section 122, and the thick-wall section 121 is elastically clamped between the pipe clamping sleeve 13 and the liquid inlet pipe 11; the thin-walled segment 122 extends beyond the port of the collet sleeve 13 for insertion operations.

With this arrangement, the sealing between the jacket sleeve 13 and the inlet pipe 11 can be enhanced by the thick-walled segment 121, and the sealing region of the two can be extended to the outside of the jacket sleeve 13 by the thin-walled segment 122.

As shown in fig. 5 to 7, in this embodiment, at least one sleeve through slot 134 is provided on the holding section 133, and the sleeve through slot 134 extends between two ports of the holding section 133.

After the arrangement mode is adopted, the inner sealing ring 12 is convenient to install in the clamping sleeve 13, and the clamping section 133 is more convenient to narrow and deform, so that the tightness is further enhanced.

While the foregoing is directed to the preferred embodiments of the present utility model, it will be appreciated by those skilled in the art that changes and modifications may be made without departing from the principles of the utility model, such changes and modifications are also intended to be within the scope of the utility model.

Claims (9)

1. An automatic microfluidic chip leakage-proof liquid injection device is characterized in that,

comprises an injection mechanism and a driving mechanism;

the injection mechanism comprises a liquid inlet pipe, an inner sleeve sealing ring, a pipe clamping sleeve, a connecting seat and an inner seat sealing ring;

the liquid inlet pipe penetrates through the pipe clamping sleeve; the sleeve inner sealing ring is sleeved outside the liquid inlet pipe, the sleeve inner sealing ring is arranged at one end of the pipe clamping sleeve for insertion operation, and the sleeve inner sealing ring is elastically clamped between the pipe clamping sleeve and the liquid inlet pipe; the outer peripheral wall of the pipe clamping sleeve is provided with external threads; the connecting seat is provided with a guide channel which penetrates through the connecting seat, and the inner peripheral wall of the guide channel is provided with internal threads; the seat inner sealing ring is arranged in the axial direction of the guide channel, and is arranged adjacent to the outlet of the guide channel;

the driving mechanism is used for controlling the injection mechanism to move and rotate so that the pipe clamping sleeve is rotationally inserted into and removed from the guide channel in a threaded connection mode, and the inserted state of the pipe clamping sleeve is elastically clamped between the seat inner sealing ring and the sleeve inner sealing ring.

2. The automated microfluidic chip leakage prevention liquid injection device according to claim 1, wherein,

the guide channel comprises a thread section and a narrowing section which are mutually communicated, the inner peripheral wall of the thread section is provided with the internal thread, and the inner diameter of the narrowing section is narrowed and changed in the direction from the thread section to the seat inner sealing ring;

the clamping pipe sleeve comprises a connecting section and a clamping section which are mutually communicated; the outer peripheral wall of the connecting section is provided with the external thread; the inner diameter of the clamping section is narrowed and changed along the direction that the clamping sleeve is inserted into the guide channel, and the inner sealing ring in the sleeve is elastically clamped in the clamping section;

and the narrowing of the narrowing is greater than the narrowing of the clamping section.

3. The automated microfluidic chip leakage prevention liquid injection device according to claim 2, wherein an inner diameter of the in-seat seal ring changes narrowly in a direction from the guide channel toward the in-seat seal ring.

4. The automated microfluidic chip leakage prevention liquid injection device according to claim 3, wherein,

the seal ring in the seat comprises a reducing section and a constant diameter section which are communicated with each other;

the diameter-variable section is communicated with the guide channel, and the inner diameter of the diameter-variable section is narrowed and changed in the direction from the guide channel to the constant diameter section;

the inner diameter of the constant diameter section is constant.

5. The automated microfluidic chip leakage prevention liquid injection device according to claim 4, wherein,

the sleeve inner sealing ring comprises a thick-wall section and a thin-wall section which are communicated with each other;

the wall thickness of the thick-wall section is larger than that of the thin-wall section, and the thick-wall section is elastically clamped between the pipe clamping sleeve and the liquid inlet pipe;

the thin-wall section extends out of the port of the clip tube sleeve for insertion operation.

6. The automated microfluidic chip leakage prevention liquid injection device according to claim 2, wherein the clamping section is provided with at least one through sleeve groove, and the through sleeve groove extends between two ports of the clamping section.

7. The automated microfluidic chip leakage prevention liquid injection device according to claim 1, wherein,

the driving mechanism comprises a moving unit and a rotating unit;

the moving unit is used for driving the rotating unit and the injection mechanism to synchronously translate and lift;

the rotating unit clamps the peripheral wall of the pipe clamping sleeve in an interference fit mode, and is used for controlling the pipe clamping sleeve to rotate.

8. The automated microfluidic chip leakage prevention liquid injection device according to claim 7, wherein,

the rotating unit comprises a rotating motor, a transmission gear and a flat key;

the rotating motor is used for controlling the rotation of the transmission gear by utilizing gear transmission;

the transmission gear is sleeved outside the pipe clamping sleeve, and a mounting groove is formed in the inner wall, facing the pipe clamping sleeve, of the transmission gear;

the flat key is arranged in the mounting groove and clamped between the transmission gear and the pipe clamping sleeve.

9. The automated microfluidic chip leakage prevention and injection device according to any one of claims 1 to 8, further comprising a clamping mechanism, wherein the clamping mechanism comprises clamping blocks, guide posts and air cylinders, the clamping blocks are respectively arranged on two opposite sides of the connecting seat, the clamping blocks are respectively inserted into the connecting seat by utilizing the guide posts in a sliding manner, two telescopic arms of the air cylinders are respectively connected with the clamping blocks, and the air cylinders are used for driving the clamping blocks to move in opposite directions and in opposite directions.