CN220618963U - Car-T cell culture detection kit - Google Patents
Car-T cell culture detection kit Download PDFInfo
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- CN220618963U CN220618963U CN202322161304.3U CN202322161304U CN220618963U CN 220618963 U CN220618963 U CN 220618963U CN 202322161304 U CN202322161304 U CN 202322161304U CN 220618963 U CN220618963 U CN 220618963U
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- 238000004113 cell culture Methods 0.000 title claims abstract description 25
- 238000001514 detection method Methods 0.000 title abstract description 11
- 238000012360 testing method Methods 0.000 claims abstract description 69
- 239000003153 chemical reaction reagent Substances 0.000 claims abstract description 26
- 238000007789 sealing Methods 0.000 claims abstract description 26
- ATJFFYVFTNAWJD-UHFFFAOYSA-N Tin Chemical compound [Sn] ATJFFYVFTNAWJD-UHFFFAOYSA-N 0.000 claims abstract description 20
- 239000001963 growth medium Substances 0.000 claims abstract description 10
- 238000005192 partition Methods 0.000 claims abstract description 4
- 238000003149 assay kit Methods 0.000 claims description 8
- 239000007788 liquid Substances 0.000 claims description 8
- 238000012258 culturing Methods 0.000 claims description 7
- 239000003795 chemical substances by application Substances 0.000 claims description 4
- 239000012528 membrane Substances 0.000 claims description 4
- 238000011010 flushing procedure Methods 0.000 claims description 3
- 239000003086 colorant Substances 0.000 claims description 2
- 210000004027 cell Anatomy 0.000 description 15
- 238000002474 experimental method Methods 0.000 description 6
- 239000012578 cell culture reagent Substances 0.000 description 4
- 238000000034 method Methods 0.000 description 3
- 102100037850 Interferon gamma Human genes 0.000 description 2
- 108010074328 Interferon-gamma Proteins 0.000 description 2
- 210000001744 T-lymphocyte Anatomy 0.000 description 2
- 238000000338 in vitro Methods 0.000 description 2
- 108090000623 proteins and genes Proteins 0.000 description 2
- 108091007741 Chimeric antigen receptor T cells Proteins 0.000 description 1
- 238000008157 ELISA kit Methods 0.000 description 1
- 206010028980 Neoplasm Diseases 0.000 description 1
- 230000004075 alteration Effects 0.000 description 1
- 239000000427 antigen Substances 0.000 description 1
- 102000036639 antigens Human genes 0.000 description 1
- 108091007433 antigens Proteins 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000004663 cell proliferation Effects 0.000 description 1
- 238000004140 cleaning Methods 0.000 description 1
- 238000010276 construction Methods 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 102000037865 fusion proteins Human genes 0.000 description 1
- 108020001507 fusion proteins Proteins 0.000 description 1
- 239000012535 impurity Substances 0.000 description 1
- 230000003834 intracellular effect Effects 0.000 description 1
- 239000003550 marker Substances 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 238000010186 staining Methods 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 230000026683 transduction Effects 0.000 description 1
- 238000010361 transduction Methods 0.000 description 1
- 238000012546 transfer Methods 0.000 description 1
- 210000004881 tumor cell Anatomy 0.000 description 1
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- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The utility model relates to the technical field of cell culture kits, and discloses a Car-T cell culture detection kit which comprises the following components: the box body is hinged with a box cover on the outer wall of one side of the box body, a culture bin is arranged on one side of the interior of the box body, a test bin is arranged on the other side of the interior of the box body through a partition board, and a support is arranged in the culture bin and the test bin, and a culture tube is embedded on the upper surface of the middle of the support; the culture kit is fixed on the inner surface of one side of the culture bin, and a culture reagent set consisting of a culture medium, a protein transporter, a cell rupture fixing solution and a corresponding antibody is filled in the culture kit. The Car-T cell culture detection kit is provided with the sealing tinfoil, and is used for sealing and preserving the culture reagent set and the test reagent set when the culture reagent set and the test reagent set are not opened, so that a worker can conveniently culture and test the Car-T cell culture detection kit in the world step by step, the functionality and the applicability are better, and the operation convenience is higher.
Description
Technical Field
The utility model relates to the technical field of cell culture kits, in particular to a Car-T cell culture detection kit.
Background
Chimeric antigen receptor T cells are T cells which can target specific tumor-associated antigens and antibody binding regions, are coupled with the CD 3-zeta chain or the intracellular part of FcεRIgamma to form a chimeric protein in vitro, are transfected by a gene transduction method, specifically target and kill tumor cells, and are a 'recoded' T cell which needs to be cultured in vitro in a large quantity and is returned to the body of a patient after being detected to be qualified.
Through searching, a paper ELISA kit for detecting IFN-gamma release of the Car-T cells, which has the publication number of CN205861688U, comprises a box body, wherein a standard tube, a treatment liquid tube and a cleaning liquid tube are sequentially arranged in the inner cavity of the box body from left to right, and are used for accurately detecting the IFN-gamma release of the Car-T cells.
Disclosure of Invention
The utility model aims to provide a Car-T cell culture detection kit for solving the problems in the background technology.
In order to achieve the above purpose, the present utility model provides the following technical solutions: a Car-T cell culture assay kit comprising:
the box body is hinged with a box cover on the outer wall of one side of the box body, a culture bin is arranged on one side of the interior of the box body, a test bin is arranged on the other side of the interior of the box body through a partition plate, supports are arranged in the culture bin and the test bin, finger holes are formed in the upper surfaces of the two sides of the supports, and a culture tube is embedded in the upper surface of the middle of the support;
the culture kit is fixed on the inner surface of one side of the culture bin, a culture reagent set consisting of a culture medium, a protein transfer agent, a cell rupture membrane fixing liquid and a corresponding antibody is filled in the culture kit, a test kit is fixed on the inner surface of one side of the test bin, a test reagent set consisting of the culture medium, the cell rupture membrane fixing liquid, a flushing agent and a coloring agent is filled in the test kit, and sealing tinfoil is paved on the upper surfaces of the culture kit and the test kit.
Preferably, the cultivating bin and the testing bin are symmetrically arranged about the central axis of the box body, the bracket is matched with the external dimensions of the cultivating bin and the testing bin, and the finger holes are symmetrically arranged about the central axis of the bracket.
Preferably, the sealing tinfoil forms a sealing structure between the culture kit and the culture kit, forms a sealing structure between the test kit and the test kit, and forms a detachable structure between the sealing tinfoil and the culture kit and the test kit.
Preferably, the four side inner walls of cultivate storehouse and test storehouse all are fixed with the fixture block, the lid is close to the internal surface fixation of box body one end has the lens hood, the lens hood is kept away from the one end outer wall cover of lid and is equipped with the rubber ring.
Preferably, the fixture blocks are symmetrically arranged on the central axis positions of the culture bin and the test bin, the support is connected with the culture bin and the test bin in a clamping way through the fixture blocks, and the light shield is connected with the culture tube in a sealing way through the support.
Preferably, a buckle is fixed on the outer wall of one side of the box cover, a fixing bolt is installed on the outer wall of one side of the box body close to the buckle, and a buckle plate is sleeved at the joint of the fixing bolt and the box body.
Preferably, the buckle plate is connected with the box body in a rotating way through a fixing bolt, and the box cover is connected with the box body in a buckling way through the buckle plate and the buckle.
Compared with the prior art, the utility model has the beneficial effects that:
the Car-T cell culture detection kit is provided with the sealing tinfoil, and is used for sealing and preserving the culture reagent set and the test reagent set when the culture reagent set and the test reagent set are not opened, so that a worker can conveniently culture and test the Car-T cell culture detection kit in the world step by step, the functionality and the applicability are better, and the operation convenience is higher.
Drawings
FIG. 1 is a schematic view showing the structure of the box cover in an opened state;
FIG. 2 is a schematic view of a portion of a cartridge body in a rack removed state according to the present utility model;
FIG. 3 is a schematic view of a front view of a bracket portion of the present utility model;
FIG. 4 is a schematic diagram of a portion of a kit of the present utility model;
fig. 5 is an enlarged view of the buckle and the buckle disassembly state according to the present utility model.
In the figure: 1. a case body; 2. a box cover; 3. a culture bin; 4. a test bin; 5. a bracket; 6. finger holes; 7. a culture tube; 8. a culture kit; 9. a test kit; 10. sealing the tin foil; 11. culturing a reagent group; 12. a test reagent set; 13. a clamping block; 14. a light shield; 15. a rubber ring; 16. a buckle; 17. a fixing bolt; 18. the buckle plate.
Detailed Description
The following description of the embodiments of the present utility model will be made clearly and completely with reference to the accompanying drawings, in which it is apparent that the embodiments described are only some embodiments of the present utility model, but not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the utility model without making any inventive effort, are intended to be within the scope of the utility model.
The present utility model provides a detection kit for Car-T cell culture by improvement, please refer to fig. 1-2 and 4, comprising: the cell culture device comprises a box body 1, wherein a box cover 2 is hinged to the outer wall of one side of the box body 1, a culture bin 3 is arranged on one side of the interior of the box body 1, a test bin 4 is arranged on the other side of the interior of the box body 1 through a partition plate, a support 5 is symmetrically arranged in the culture bin 3 and the test bin 4 about the central axis of the box body 1, the support 5 is matched with the external dimensions of the culture bin 3 and the test bin 4, finger holes 6 are formed in the upper surfaces of two sides of the support 5, the finger holes 6 are symmetrically arranged about the central axis of the support 5, a worker conveniently takes and places the support 5 from the culture bin 3 or the test bin 4, a culture tube 7 is embedded in the upper surface of the middle of the support 5, a culture reagent kit 8 is fixed on the inner surface of one side of the culture bin 3, a culture reagent kit 11 composed of a culture medium, a protein transporter, a cell rupture fixing solution and a corresponding antibody is fixed on the inner surface of one side of the test bin 4, a test reagent kit 9 is filled with the culture medium, a cell rupture fixing solution, a test reagent 12 composed of a cell rupture fixing solution and a dye is filled in the inner surface of the test kit 9, and a test reagent kit 12 is used for improving the cell culture reagent set 12 and the cell culture reagent 1 in the test reagent set and the test reagent kit 1; the sealed tinfoil 10 has all been laid to culture kit 8 and test kit 9 upper surface, sealed tinfoil 10 constitutes seal structure through between culture kit 8 and the culture kit group 11, and sealed tinfoil 10 constitutes seal structure through between test kit 9 and the test kit group 12, and constitute detachable construction between sealed tinfoil 10 and culture kit 8 and the test kit 9, sealed tinfoil 10 that sets up is used for carrying out sealed save to culture kit group 11 and test kit group 12 when culture kit 8 and test kit 9 are unopened, the staff of being convenient for uses this world and step by step to cultivate and test the operation, the functionality and suitability are better, the simple operation is higher.
Referring to FIGS. 1-3, a test kit for culturing Car-T cells, comprising: clamping blocks 13 are fixed on the four inner walls of the culture bin 3 and the test bin 4, the clamping blocks 13 are symmetrically arranged on the central axis positions of the culture bin 3 and the test bin 4, the bracket 5 is connected with the culture bin 3 and the test bin 4 in a clamping way through the clamping blocks 13, a light shield 14 is fixed on the inner surface of one end of the box body 1, which is close to the box cover 2, a sealing structure is formed between the light shield 14 and the culture tube 7 through the bracket 5, the light shield 14 is used for providing secondary sealing and shading effects for the upper end of the culture tube 7, the sealing performance of the culture tube 7 when being closed is improved, and the interference of the external environment to the culture tube 7 is reduced; the outer wall of one end of the light shield 14 far away from the box cover 2 is sleeved with a rubber ring 15, and the rubber ring 15 is used for improving the sealing performance of the light shield 14 on the opening end of the culture tube 7 when the light shield 14 covers the upper end of the culture tube 7, so that impurities and the like are further reduced and enter the light shield 14.
Referring to FIGS. 1-2 and 5, a test kit for culturing a Car-T cell, comprising: the buckle 16 is fixed on one side outer wall of the box cover 2, the fixing bolt 17 is installed on one side outer wall of the box body 1 close to the buckle 16, the buckle 18 is sleeved at the joint of the fixing bolt 17 and the box body 1, the buckle 18 is connected with the box body 1 in a rotating way through the fixing bolt 17, the box cover 2 is connected with the box body 1 in a buckling way through the buckle 18 and the buckle 16, the buckle 18 and the buckle 16 are used for fastening the box body 1 and the box cover 2, and the phenomenon that the box cover 2 accidentally opens in the transferring or transporting process to lead to the sliding out of the culture tube 7 and the bracket 5 is prevented.
Working principle: for the Car-T cell culture detection kit, firstly, a worker dials the buckle plate 18 from the buckle 16 on the box cover 2, the buckle plate 18 rotates at the joint of the fixing bolt 17 and the box body 1 to release the box cover 2, after the box cover 2 is opened, the worker respectively uses experimental materials in the culture bin 3 and the test bin 4 according to an experimental process, a culture tube 7 for culturing Car-T cells is arranged in the culture bin 3 through the support 5, after the operator takes out the support 5 through the finger hole 6, the Car-T cells can be cultured by using the culture reagent group 11 which is contained in the culture medium and the culture reagent box 8 through the sealing tinfoil 10, the culture medium, the protein transporter, the cell rupture fixing liquid and the corresponding antibody are respectively used for cell proliferation, when the target cells correspond to the antibody cell culture, and then the test operation is needed, the worker takes out the support 5 in the test bin 4, the cells in the test reagent group 12 which are contained in the culture tube 7 are tested by the practical test reagent box 9, the culture medium, the cell rupture fixing liquid, the flushing and the staining agent are respectively used for cell culture, the cell culture marker and the cell culture reagent are respectively, finally the cell culture reagent are arranged on the support 7 through the sealing ring 2 and the support 5, and the light shielding ring is further arranged in the experiment bin 2 and the experiment box cover 2 is closed through the sealing ring 13 and the sealing ring 13, and the experiment opening is further arranged in the support 2 and the support 7 is closed, and the experiment opening is further covered by the support 2, and the experiment opening is opened and the experiment end is covered by the support 2.
Although embodiments of the present utility model have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made therein without departing from the principles and spirit of the utility model, the scope of which is defined in the appended claims and their equivalents.
Claims (7)
1. A Car-T cell culture assay kit comprising:
the novel multifunctional incubator comprises an incubator body (1), wherein a case cover (2) is hinged to the outer wall of one side of the incubator body (1), a culturing bin (3) is arranged on one side of the inside of the incubator body (1), a testing bin (4) is arranged on the other side of the inside of the incubator body (1) through a partition plate, a bracket (5) is arranged in each of the culturing bin (3) and the testing bin (4), finger holes (6) are formed in the upper surfaces of two sides of the bracket (5), and a culturing tube (7) is embedded in the upper surface of the middle of the bracket (5);
the culture kit (8) is fixed on one side inner surface of the culture bin (3), a culture reagent group (11) consisting of a culture medium, a protein transporter, a cell rupture membrane fixing liquid and a corresponding antibody is filled in the culture kit (8), a test kit (9) is fixed on one side inner surface of the test bin (4), a test reagent group (12) consisting of the culture medium, the cell rupture membrane fixing liquid, a flushing agent and a coloring agent is filled in the test kit (9), and sealing tinfoil (10) is paved on the upper surfaces of the culture kit (8) and the test kit (9).
2. The Car-T cell culture assay kit of claim 1, wherein: the culture bin (3) and the test bin (4) are symmetrically arranged on the central axis of the box body (1), the support (5) is matched with the outline dimensions of the culture bin (3) and the test bin (4), and the finger holes (6) are symmetrically arranged on the central axis of the support (5).
3. The Car-T cell culture assay kit of claim 1, wherein: the sealing tinfoil (10) forms a sealing structure between the culture kit (8) and the culture kit (11), the sealing tinfoil (10) forms a sealing structure between the test kit (9) and the test kit (12), and a detachable structure is formed between the sealing tinfoil (10) and the culture kit (8) and the test kit (9).
4. The Car-T cell culture assay kit of claim 1, wherein: the four-side inner walls of the culture bin (3) and the test bin (4) are respectively fixed with a clamping block (13), the inner surface of the box cover (2) close to one end of the box body (1) is fixedly provided with a light shield (14), and the outer wall of one end of the light shield (14) far away from the box cover (2) is sleeved with a rubber ring (15).
5. The Car-T cell culture assay kit of claim 4, wherein: the fixture blocks (13) are symmetrically arranged on the central axis positions of the culture bin (3) and the test bin (4), the support (5) is connected with the culture bin (3) and the test bin (4) in a clamping mode through the fixture blocks (13), and the light shield (14) is of a sealing structure with the culture tube (7) through the support (5).
6. The Car-T cell culture assay kit of claim 1, wherein: the box comprises a box body and is characterized in that a buckle (16) is fixed on the outer wall of one side of the box cover (2), a fixing bolt (17) is installed on the outer wall of one side, close to the buckle (16), of the box body (1), and a buckle plate (18) is sleeved at the joint of the fixing bolt (17) and the box body (1).
7. The Car-T cell culture assay kit of claim 6, wherein: the buckle plate (18) is connected with the box body (1) in a rotating way through the fixing bolt (17), and the box cover (2) is connected with the box body (1) in a buckling way through the buckle plate (18) and the buckle (16).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202322161304.3U CN220618963U (en) | 2023-08-11 | 2023-08-11 | Car-T cell culture detection kit |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202322161304.3U CN220618963U (en) | 2023-08-11 | 2023-08-11 | Car-T cell culture detection kit |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN220618963U true CN220618963U (en) | 2024-03-19 |
Family
ID=90212485
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202322161304.3U Active CN220618963U (en) | 2023-08-11 | 2023-08-11 | Car-T cell culture detection kit |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN220618963U (en) |
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2023
- 2023-08-11 CN CN202322161304.3U patent/CN220618963U/en active Active
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