CN220550183U - Matrix type temperature control auxiliary heating device for PCR instrument and PCR instrument - Google Patents
Matrix type temperature control auxiliary heating device for PCR instrument and PCR instrument Download PDFInfo
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- CN220550183U CN220550183U CN202322092173.8U CN202322092173U CN220550183U CN 220550183 U CN220550183 U CN 220550183U CN 202322092173 U CN202322092173 U CN 202322092173U CN 220550183 U CN220550183 U CN 220550183U
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- 238000010438 heat treatment Methods 0.000 title claims abstract description 335
- 239000011159 matrix material Substances 0.000 title claims abstract description 36
- 238000012546 transfer Methods 0.000 abstract description 6
- 230000008859 change Effects 0.000 abstract description 3
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- 238000000034 method Methods 0.000 description 13
- 230000008569 process Effects 0.000 description 13
- 238000010586 diagram Methods 0.000 description 8
- 230000004543 DNA replication Effects 0.000 description 6
- 238000001514 detection method Methods 0.000 description 6
- 238000000338 in vitro Methods 0.000 description 5
- 238000004925 denaturation Methods 0.000 description 4
- 230000036425 denaturation Effects 0.000 description 4
- 238000006243 chemical reaction Methods 0.000 description 3
- 230000000295 complement effect Effects 0.000 description 3
- 230000000694 effects Effects 0.000 description 3
- 230000017525 heat dissipation Effects 0.000 description 3
- 239000000463 material Substances 0.000 description 3
- 238000004153 renaturation Methods 0.000 description 3
- 239000004020 conductor Substances 0.000 description 2
- 238000001816 cooling Methods 0.000 description 2
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- 239000002184 metal Substances 0.000 description 1
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- 230000004048 modification Effects 0.000 description 1
- 238000003199 nucleic acid amplification method Methods 0.000 description 1
- 230000003287 optical effect Effects 0.000 description 1
- 238000003752 polymerase chain reaction Methods 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 230000010076 replication Effects 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L7/00—Heating or cooling apparatus; Heat insulating devices
- B01L7/52—Heating or cooling apparatus; Heat insulating devices with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples
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Abstract
The utility model discloses a matrix type temperature control auxiliary heating device for a PCR instrument and the PCR instrument, wherein the matrix type temperature control auxiliary heating device for the PCR instrument comprises a temperature control module, an auxiliary heating module, a control module and a temperature sensor, wherein a containing groove on the temperature control module is used for placing a sample tube, so that the temperature of the sample tube can be regulated in the containing groove, the control module is used for controlling the temperature of the temperature control module, and then the control module can control the temperature of a column body; offer the through-hole that is matrix distribution on the auxiliary heating module for during the matrix control by temperature change auxiliary heating device installation of PCR appearance sample pipe can run through the through-hole and stretch into the holding tank, when the heater heats, can heat the cylinder through heat transfer, can play auxiliary heating's purpose, thereby make the inside temperature of a plurality of cylinders more even, and then can make a plurality of sample pipes temperature homogeneity at the same moment better, finally make the accuracy of sample pipe testing result better.
Description
Technical Field
The utility model relates to the technical field of biological detection, in particular to a matrix type temperature control auxiliary heating device for a PCR instrument and the PCR instrument.
Background
The PCR instrument, i.e., the polymerase chain reaction analyzer, can be divided into two general classes from the module composition, one class consisting of only the temperature control device and the other class consisting of the temperature control device and the optical detection device. The main function of the temperature control device is to provide a proper temperature environment for the in-vitro DNA replication of a sample to be tested, specifically, the in-vitro DNA replication process of the sample to be tested comprises that DNA is denatured at high temperature, double chains are opened to become single chains, at low temperature, the single chains of the DNA are combined with primers in a sample tube to be tested according to the base complementary pairing principle, and at the optimal reaction temperature of polymerase, the DNA is replicated by means of the polymerase, in the process, the temperature control device can well control the temperature between the denaturation temperature, the renaturation temperature and the extension temperature, so that the sample to be tested can complete in-vitro DNA replication, and the trace DNA can be greatly increased by repeating the three reaction steps for a plurality of times. In the execution process of the three steps, the temperature of the sample to be detected needs to be accurately controlled, otherwise, the research result will deviate, and even the detection fails.
When the existing PCR instrument heats up or cools down the sample to be detected, in the actual operation process, a large temperature difference exists among a plurality of samples to be detected, and therefore the temperature uniformity among the samples to be detected at the same time is poor. Therefore, how to improve the temperature uniformity performance among a plurality of samples to be tested in the detection process is a problem to be solved.
Disclosure of Invention
The utility model aims to provide a matrix type temperature control auxiliary heating device for a PCR instrument and the PCR instrument, which solve the problem of poor temperature uniformity.
In order to achieve the above purpose, the present utility model provides a technical solution:
a matrix-type temperature-controlled auxiliary heating device for a PCR instrument, comprising:
the temperature control module comprises a plurality of columns, and a containing groove for placing the sample tube is formed in each column so that the temperature of the sample tube can be adjusted in the containing groove;
the auxiliary heating module is connected with the temperature control module, a plurality of through holes distributed in a matrix form are formed in the auxiliary heating module, the column penetrates through the through holes, a plurality of transverse heating wires and a plurality of longitudinal heating wires are further arranged on the auxiliary heating module, the transverse heating wires are positioned on one side of one row of the through holes, one transverse heating wire corresponds to one row of the through holes, the longitudinal heating wires are positioned on one side of one row of the through holes, one longitudinal heating wire corresponds to one row of the through holes, the transverse heating wires and the longitudinal heating wires are staggered to form intersecting points, heaters are arranged at least at part of the intersecting points, and the corresponding transverse heating wires and longitudinal heating wires at the intersecting points are electrically connected with the heaters;
the control module is respectively and electrically connected with the transverse heating wires and the longitudinal heating wires, and controls the transverse heating wires and the longitudinal heating wires to be sequentially powered on and powered off, and when the power is on, the transverse heating wires, the longitudinal heating wires and the corresponding heaters form loops so that the corresponding heaters heat the corresponding columns;
the temperature sensor is electrically connected with the control module, the temperature sensor is used for detecting the temperature inside the cylinder, the temperature sensor is used for transmitting detected signals to the control module, and the temperature of the heater is controlled by the control module.
Optionally, when one of the transverse heating wires is electrified, the rest of the transverse heating wires are not electrified, and the longitudinal heating wires are electrified in sequence, so that the heater heats the cylinder one by one.
Optionally, when one of the transverse heating wires is energized, the rest of the transverse heating wires are not energized, and a plurality of the longitudinal heating wires are energized, so that the heaters heat the column body in groups.
Optionally, the energizing durations of the plurality of transverse heating wires are different or the energizing durations of the plurality of longitudinal heating wires are different, so that the heating power between the heaters is different.
Optionally, the auxiliary heating module is detachably connected with the temperature control module.
Optionally, adjacent columns are connected through a bearing table, and the bearing table is used for supporting the auxiliary heating module.
Optionally, the top of the column is higher than the height of the bearing table.
Optionally, a lightening hole is formed on the bearing table.
Optionally, the through holes on the auxiliary heating module are distributed in a matrix manner in an m×n manner, wherein m is not less than 2, and n is not less than 2.
The utility model also provides a technical scheme that:
a PCR instrument, comprising: the matrix type temperature control auxiliary heating device for the PCR instrument is positioned above the heat dissipation device;
the heater of the matrix type temperature control auxiliary heating device for the PCR instrument has different heating powers.
Compared with the prior art, the utility model has the beneficial effects that:
1. the matrix type temperature control auxiliary heating device for the PCR instrument comprises a temperature control module, an auxiliary heating module, a control module and a temperature sensor, wherein a containing groove on the temperature control module is used for placing a sample tube, so that the temperature of the sample tube can be adjusted in the containing groove, through holes distributed in a matrix form are formed in the auxiliary heating module, the sample tube can penetrate through the through holes and extend into the containing groove when the matrix type temperature control auxiliary heating device for the PCR instrument is installed, a plurality of transverse heating wires and a plurality of longitudinal heating wires are further arranged on the auxiliary heating module, the transverse heating wires and the longitudinal heating wires are distributed in a crisscross manner and form a plurality of intersecting points, heaters are arranged at least at part of the intersecting points, and the transverse heating wires and the longitudinal heating wires at the corresponding intersecting points are electrically connected with the heaters, at the moment, which is equivalent to the heaters are arranged at the periphery of the through holes, and each heater just corresponds to one column body and is arranged beside the column body when the auxiliary heating module is connected with the temperature control module; the control module controls the transverse heating wires and the longitudinal heating wires to be sequentially powered on and powered off, and when the power is on, the transverse heating wires, the longitudinal heating wires and the corresponding heaters form loops so that the corresponding heaters heat the column body; when the heater heats, the column body can be heated through heat transfer, so that the heat is transferred to the sample tubes in the column body, the purpose of auxiliary heating of the sample tubes is achieved, and therefore temperature compensation can be carried out on the sample tubes, and the problem that temperature uniformity among a plurality of sample tubes is poor is solved; the temperature sensor is used for detecting the temperature inside the column body, namely the temperature of the sample tube can be detected, then the temperature sensor can transmit detected signals to the control module, and the temperature of the heater is controlled by the control module; when the temperature control module heats samples to be tested in the sample tubes, and when the temperature sensor senses that the internal temperature of part of the cylinders does not reach the preset temperature, the control module controls the transverse heating wires and the longitudinal heating wires of the auxiliary heating module to be sequentially powered on and powered off so that the corresponding heaters heat the corresponding cylinders, and the auxiliary heating of the sample tubes is completed through the auxiliary heating module, so that the temperature uniformity performance among the sample tubes is improved.
2. When the transverse heating wire and the longitudinal heating wire of the matrix type temperature control auxiliary heating device for the PCR instrument are communicated with the heater, the heater is started; after at least one of the transverse heating wire and the longitudinal heating wire is disconnected from the heater, the heater is turned off, so that the control module can control the on-off of the heater by controlling the transverse heating wire and the longitudinal heating wire, the use is convenient, and the arrangement modes of the transverse heating wire and the longitudinal heating wire are tidy.
3. The adjacent columns in the matrix type temperature control auxiliary heating device for the PCR instrument are connected through the bearing table, the bearing table is used for supporting the auxiliary heating module, the height of the bearing table determines the position of the heater relative to the sample tube, and the position of the bearing table can be changed according to actual use requirements.
4. According to the matrix type temperature control auxiliary heating device for the PCR instrument, the bearing table is provided with the lightening holes, and the lightening holes can lighten the weight of the whole temperature control module.
5. The control module for the matrix type temperature control auxiliary heating device of the PCR instrument is also used for controlling the temperature of the temperature control module, so that the control module can control the temperature of the cylinder and can play a main heating role, when the temperature sensor senses that the internal temperature of most or all cylinders is lower, the control module can control the temperature of the temperature control module to raise the temperature of all cylinders, when the temperature sensor senses that the internal temperature of a few cylinders is lower, the control module can control the temperature of the corresponding heater to enable the heat generated by the heater to be transferred to the cylinders, and the temperature of the cylinders is raised to the target temperature, so that the internal temperatures of a plurality of cylinders are uniform, further, the temperature uniformity of a plurality of sample tubes at the same moment is better, and finally, the accuracy of the detection results of the sample tubes is better.
Additional aspects and advantages of the utility model will be set forth in part in the description which follows and, in part, will be obvious from the description, or may be learned by practice of the utility model.
Drawings
FIG. 1 is a schematic diagram of a matrix-type temperature-controlled auxiliary heating device for a PCR instrument according to the present utility model;
FIG. 2 is a schematic diagram of a first configuration of an auxiliary heating module of a matrix-type temperature-controlled auxiliary heating apparatus for a PCR instrument according to the present utility model;
FIG. 3 is a schematic diagram of a first view angle of a temperature control module of a matrix type temperature control auxiliary heating device for a PCR instrument according to the present utility model;
FIG. 4 is a schematic view of the structure of a sample tube;
FIG. 5 is a schematic diagram showing the circuit connection of the auxiliary heating module of the matrix-type temperature control auxiliary heating device for the PCR instrument;
FIG. 6 is a schematic diagram of a second view angle of a temperature control module of a matrix-type temperature control auxiliary heating device for a PCR instrument according to the present utility model;
FIG. 7 is a schematic diagram showing a second structure of an auxiliary heating module of the matrix-type temperature-controlled auxiliary heating apparatus for a PCR instrument according to the present utility model;
FIG. 8 is a schematic diagram of the structure of a PCR apparatus according to the present utility model.
In the figure:
1. a temperature control module; 11. a column; 111. a receiving groove; 12. a carrying platform; 121. a lightening hole; 13. a mounting groove; 2. an auxiliary heating module; 21. a through hole; 22. a heater; 23. a transverse heating wire; 24. a longitudinal heating wire; 3. a sample tube; 4. a heat sink.
Detailed Description
The following description of the embodiments of the present utility model will be made clearly and completely with reference to the accompanying drawings, in which it is apparent that the embodiments described are only some embodiments of the present utility model, but not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the utility model without making any inventive effort, are intended to be within the scope of the utility model.
In the present utility model, unless explicitly specified and limited otherwise, the terms "mounted," "connected," "secured," and the like are to be construed broadly and may be, for example, fixedly connected, detachably connected, or integrally connected; can be mechanically or electrically connected; can be directly connected or indirectly connected through an intermediate medium, and can be communication between two elements. The specific meaning of the above terms in the present utility model can be understood by those of ordinary skill in the art according to the specific circumstances.
In the prior art, when a sample to be tested is subjected to in vitro DNA replication, the DNA is denatured at a high temperature (about 95 ℃ and also called denaturation temperature), double chains are opened to become single chains, at a low temperature (about 60 ℃ and also called renaturation temperature), the single chains of the DNA are combined with primers in the sample to be tested according to the base complementary pairing principle, and at the optimal reaction temperature (about 72 ℃ and also called extension temperature) of the polymerase, the DNA is replicated by means of the polymerase, in the process, a temperature control module can control the denaturation temperature, renaturation temperature and extension temperature, so that the sample to be tested can be subjected to in vitro DNA replication, but when the sample to be tested is subjected to heating and heating or refrigerating and cooling treatment, the temperature difference exists among a plurality of accommodating holes on a temperature control block of the temperature control module, and the temperature uniformity is poor, so that in the actual operation process, a large temperature difference exists among a plurality of sample tubes to be tested, the temperature uniformity difference exists among a plurality of sample tubes, and finally the temperature uniformity among a plurality of samples to be tested is poor, and the experimental result is influenced.
Therefore, the embodiment provides a matrix type temperature control auxiliary heating device for a PCR instrument, which solves the problem of poor temperature uniformity among samples to be tested in the PCR experiment process.
Referring to fig. 1, an embodiment of the present disclosure provides a matrix-type temperature-controlled auxiliary heating apparatus for a PCR instrument, including a temperature control module 1, an auxiliary heating module 2, a control module, and a temperature sensor. As shown in fig. 1, the temperature control module 1 comprises a plurality of columns 11, and a containing groove 111 for placing the sample tube 3 is formed in the column 11, so that the sample tube 3 adjusts the temperature in the containing groove 111; as shown in fig. 1 and fig. 2, the auxiliary heating module 2 is connected to the temperature control module 1, specifically, the auxiliary heating module 2 is provided with a plurality of through holes 21 distributed in matrix, the column 11 penetrates through the through holes 21, so that the auxiliary heating module 2 is connected to the temperature control module 1, the auxiliary heating module 2 is further provided with a plurality of transverse heating wires 23 and a plurality of longitudinal heating wires 24, the transverse heating wires 23 are located at one side of a row of through holes 21, one transverse heating wire 23 corresponds to a row of through holes 21, the longitudinal heating wires 24 are located at one side of a row of through holes 21, one longitudinal heating wire 24 corresponds to a row of through holes 21, the transverse heating wires 23 and the longitudinal heating wires 24 are staggered to form intersecting points, at least part of intersecting points are provided with heaters 22, at least part of the transverse heating wires 23 and the longitudinal heating wires 24 at the corresponding intersecting points are electrically connected with the heaters 22, at this time, the heater 22 is equivalent to be arranged at the periphery of the through holes 21, and when the auxiliary heating module 2 is connected to the temperature control module 1, each heater 22 corresponds to exactly one column 11 and is arranged beside the column 11; the control module is respectively and electrically connected with the plurality of transverse heating wires and the plurality of longitudinal heating wires, and controls the plurality of transverse heating wires 23 and the plurality of longitudinal heating wires 24 to be sequentially powered on and powered off, and when the power is on, the transverse heating wires 23, the longitudinal heating wires 24 and the corresponding heaters 22 form loops so that the corresponding heaters 22 heat the corresponding columns 11.
It should be noted that the intersection point formed by the staggered arrangement of the transverse heating wires 23 and the longitudinal heating wires 24 is only a virtual intersection point where the transverse heating wires 23 and the longitudinal heating wires 24 are not connected, and is not a circuit welding point where the transverse heating wires 23 and the longitudinal heating wires 24 are electrically connected together, and the heater 22 is disposed at the virtual intersection point, and the heater 22 is electrically connected to the transverse heating wires 23 and the longitudinal heating wires 24 at the same point, respectively, as shown in fig. 5.
When the auxiliary heating module 2 is not connected with the temperature control module 1, as shown in fig. 3 and 4, the sample tubes 3 can be directly embedded in the column 11 of the temperature control module 1, and the control module or other heating devices can control the temperature control module 1 to perform main heating on the sample tubes 3, however, in this mode, temperature differences exist between the plurality of sample tubes 3, so that the temperature uniformity is poor, and the experimental result is affected. And this application is through setting up auxiliary heating module 2, after auxiliary heating module 2 is connected with temperature control module 1 and accomplishes, be the state as shown in fig. 1, at this moment, hold the sample pipe 3 of sample that awaits measuring and run through hole 21 and stretch into in the holding tank 111 of cylinder 11, thereby accomplish the placing of sample pipe 3, because the periphery of every through hole 21 all encloses and establish heater 22, therefore all enclose the periphery of every cylinder 11 and establish heater 22, when heater 22 heats, can heat cylinder 11 through heat transfer, and then with the sample pipe 3 in the heat transfer cylinder 11, play the purpose to the auxiliary heating of sample pipe 3, thereby can carry out temperature compensation to sample pipe 3, with the relatively poor problem of temperature homogeneity between a plurality of sample pipes 3.
The temperature sensor is electrically connected with the control module and is used for detecting the temperature inside the column 11, namely, the temperature of the sample tubes 3 can be detected, then the temperature sensor can transmit detected signals to the control module, the control module is used for controlling the temperature of the heater 22, namely, when the temperature control module 1 heats up samples to be detected in the sample tubes 3, and when the temperature sensor senses that the internal temperature of part of the column 11 does not reach the preset temperature (such as denaturation temperature), the control module controls the transverse heating wires 23 and the longitudinal heating wires 24 of the auxiliary heating module 2 to be sequentially powered on and powered off, so that the corresponding column 11 is heated by the corresponding heater 22, the auxiliary heating of the sample tubes 3 is completed through the auxiliary heating module 2, and the temperature uniformity performance among the sample tubes 3 is improved.
The principle of auxiliary heating of the auxiliary heating module 2 is described in detail below.
As shown in fig. 5, a schematic diagram of a connection circuit of the lateral heating wire 23, the longitudinal heating wire 24 and the heater 22 of the auxiliary heating module 2 is shown, wherein one end of the lateral heating wire 23 is used for being electrically connected or disconnected with the control module, the other end of the lateral heating wire is not connected with a circuit, one end of the longitudinal heating wire is used for being electrically connected or disconnected with the control module, and the other end of the longitudinal heating wire is not connected with the circuit, wherein the control module internally comprises a control program and a power supply, the positive electrode of the control program controls the power supply to be communicated with the lateral heating wire 23, and the negative electrode of the control power supply is communicated with the longitudinal heating wire 24, so that the lateral heating wire 23, the longitudinal heating wire 24 and the corresponding heater 22 form a loop, the heater 22 heats the corresponding column 11, the temperature of the column 11 is raised to the target temperature, the internal temperatures of the columns 11 are uniform, and the temperature uniformity of the sample tubes 3 at the same time is good; after the control module controls the disconnection of at least one of the transverse heating wire 23 and the longitudinal heating wire 24 and the heater 22, the heater 22 is turned off, so that the control module can control the on-off of the heater 22 by controlling the transverse heating wire 23 and the longitudinal heating wire 24, and the on-off of the plurality of heaters 22 can be controlled by means of a very small number of heating wires by controlling the connection mode of the transverse heating wire 23 and the longitudinal heating wire 24 in a longitudinal-transverse arrangement and the control mode of the control module, thereby saving the cost and being more convenient to operate.
Various auxiliary heating modes of the auxiliary heating module 2 are specifically described below.
The heating mode is as follows: the plurality of heaters 22 are individually activated and individually heated.
Specifically, in some embodiments, when one of the lateral heating wires 23 is energized, the remaining lateral heating wires 23 are not energized, and the longitudinal heating wires 24 are energized sequentially, so that the heater 22 heats the column 11 one by one (sequentially). For example, the control module controls the first transverse heater wire 23 to be energized, the rest not energized, then the longitudinal heater wires 24 are energized one by one, and only one longitudinal heater wire 24 is energized at a time, i.e., when the first longitudinal heater wire 24 is energized, the rest of the longitudinal heater wires 24 are all de-energized, when the second longitudinal heater wire 24 is energized, the first and other longitudinal heater wires 24 are all de-energized, when the third longitudinal heater wire 24 is energized, the first, second and other pairs of longitudinal heater wires 24 are all de-energized, and so on, regardless of how many longitudinal heater wires 24 have, all are energized in the manner described above. When the heating of the first row of heaters 22 is completed, the second transverse heating wire 23 is electrified, the first transverse heating wire 23 and the other transverse heating wires 23 are powered off, then the longitudinal heating wires 24 are electrified one by one, and only one longitudinal heating wire 24 is electrified at the same time, so that no matter how many transverse heating wires 23 are, the heaters 22 can be powered on or off in the mode, and the heaters 22 can be heated one by one. The current-carrying mode corresponds to that after each transverse heating wire 23 is heated for a period of time, the longitudinal heating wires 24 are all electrified one by one. Through this kind of matrix control by temperature change auxiliary heating device that this application provided, can make a plurality of heaters 22 carry out the individual heating to corresponding cylinder 11 one by one to when avoiding a plurality of heaters 22 to start simultaneously, take place heat transfer between the cylinder 11, with the auxiliary heating effect of influence cylinder 11, thereby improve in the experimentation, the temperature homogeneity between the sample pipe 3 improves the accuracy of experimental result.
The control module controls the on-off sequence of the transverse heating wire 23 and the longitudinal heating wire 24, and the heating power of each heater 22 is recorded and stored in the control program of the control module after design. For example, the order of heating the heaters 22 one by one may be from the upper left corner shown in fig. 5, from left to right, from top to bottom, from the lower right corner shown in fig. 5, from right to left, from bottom to top, or other heating orders, as long as the heaters 22 are ensured to heat the corresponding columns 11 one by one; in addition, the energization time of each of the lateral heating wires 23 or each of the longitudinal heating wires 24 may be different, so that the energization time of each of the heaters 22 is different to change the heating power of each of the heaters 22, thereby further improving the temperature uniformity performance between the sample tubes 3. In another heating mode, the heaters 22 are configured in groups, each group includes at least two heaters 22, and the heaters 22 are started in groups and heated in groups.
Specifically, in some embodiments, when one of the lateral heater wires 23 is energized, the remaining lateral heater wires 23 are not energized, and a plurality of longitudinal heater wires 24 are energized to group the heaters 22 to heat the columns 11. For example, the control module controls the first transverse heating wire 23 to be energized, the rest not energized, then a plurality of longitudinal heating wires 24 are energized, and the plurality of longitudinal heating wires 24 may be grouped such that the longitudinal heating wires 24 may be energized and de-energized in groups, i.e., when the first group of longitudinal heating wires 24 is energized, the rest of the longitudinal heating wires 24 are de-energized, when the second group of longitudinal heating wires 24 is energized, the first group and the other longitudinal heating wires 24 are de-energized, and so on, no matter how many groups the longitudinal heating wires 24 have, the above-described manner is employed to energize and de-energize. When the heating of the first row of heaters 22 is completed, the second transverse heating wires 23 are energized, the first and other transverse heating wires 23 are de-energized, then the longitudinal heating wires 24 are energized in groups, and thus, no matter how many transverse heating wires 23 are, the heaters 22 can be heated in groups by energizing in the manner described above. The energization mode corresponds to that after each of the lateral heating wires 23 is heated for a certain period of time, the longitudinal heating wires 24 are energized in groups.
In other embodiments, when the plurality of lateral heating wires 23 are energized, the remaining lateral heating wires 23 are not energized, and the longitudinal heating wires 24 are energized in sequence to heat the columns 11 in groups of heaters 22. For example, the control module controls the plurality of transverse heating wires 23 to be energized and the rest not energized, and the plurality of transverse heating wires 23 may be grouped so that the transverse heating wires 23 may be energized in groups, then the longitudinal heating wires 24 are energized one by one, and only one longitudinal heating wire 24 is energized at a time, i.e., when a first longitudinal heating wire 24 is energized, the rest of the longitudinal heating wires 24 are de-energized, when a second longitudinal heating wire 24 is energized, the first and other longitudinal heating wires 24 are de-energized, and so on, no matter how many longitudinal heating wires 24 have, the above-described manner is employed. When the heating of the plurality of rows of heaters 22 is completed, the second group of transverse heating wires 23 is energized, the first group of transverse heating wires 23 and the other transverse heating wires 23 are deenergized, then the longitudinal heating wires 24 are energized one by one, and only one longitudinal heating wire 24 is energized at the same time, so that no matter how many transverse heating wires 23 are, the heaters 22 can be heated in groups by energizing in the above manner.
By the matrix type temperature control auxiliary heating device provided by the application, a plurality of heaters 22 can heat the corresponding column 11 at the same time so as to improve the auxiliary heating rate.
In other embodiments, the control module may control the transverse heating wires 23 and the longitudinal heating wires 24 to sequentially heat from outside to inside, at which time the two outer longitudinal heating wires 24 are energized, the transverse heating wires 23 are energized one by one, that is, the outer longitudinal heating wires 24 are energized for a period of time and then disconnected (each time the longitudinal heating wires 24 are energized for a period of time, the transverse heating wires 23 are energized one by one), and then the inner longitudinal heating wires 24 are also energized for a period of time and then disconnected, so as to heat the entire column 11. The heating power of the outer heater 22 may be larger than the heating power of the inner heater 22 during heating.
In some embodiments, the energizing durations of the plurality of lateral heating wires 23 are different or the energizing durations of the plurality of longitudinal heating wires 24 are different, such that the heating power is different between the heaters 24. The control module is mainly used for controlling the transverse heating wire 23 and the longitudinal heating wire 24 to be simultaneously electrified and then not to be simultaneously powered off, so that different heaters 22 can have different heating times, and the non-heating power of different sample tubes 3 is realized.
In the temperature regulation process, the heaters 22 at the intersections are sequentially and electrically heated one by one or are electrically heated one by one in groups so as to perform auxiliary heating on the sample tubes 3 one by one or one by one, thereby enabling the temperature of the sample tubes 3 to reach the same temperature and improving the temperature uniformity among a plurality of sample tubes 3 and samples to be measured.
It should be noted that the above steps only give some possible working processes of the temperature control module 1, and that other working processes of the temperature control module 1 are possible.
The heaters 22 are operated one by one in the optimal scheme, and the purpose of the heaters 22 operated one by one is to further improve the temperature uniformity of the sample tube 3, prevent heat conduction between the two heaters 22 when they are operated simultaneously, thereby affecting the effect of auxiliary heating and further improving the temperature control accuracy.
It should be noted that the heating power of each heater 22 is not uniform when it is operated because the temperature of each sample tube 3 is not uniform when there is a problem of temperature uniformity between columns 11, and the heating power is naturally not uniform when auxiliary heating is performed to each sample tube 3 in order to raise the temperatures of all sample tubes 3 to the same temperature. The control module, when controlling the energization of each longitudinal heating wire 24 and each transverse heating wire 23, designs and executes the energization power according to the heating power required by the sample tubes 3 at the intersection point, and when some of the sample tubes 3 do not need to be subjected to auxiliary heating, either or both of the transverse heating wire 23 and the longitudinal heating wire 24 located at the sample tube 3 are not energized, so that the heating power of the heater 22 at the location is zero.
For example, since the number of the transverse heating wires 23 is 12 and the number of the longitudinal heating wires 24 is 8, 96 intersections are formed, 96 heaters 22 can be designed and distributed in a matrix, and 96 sample tubes 3 are also arranged and distributed in a matrix, which corresponds to the heaters 22 surrounding the sample tubes 3. The control module adopts a Pulse Width Modulation (PWM) technology to control the transverse heating wire 23 and the longitudinal heating wire 24, and is designed to complete one auxiliary heating of 96 sample tubes 3 in 1 second or 2 seconds, so that one auxiliary heating of 96 sample tubes 3 can be completed in a very short time, which approximates to simultaneous auxiliary heating of 96 sample tubes 3, and further improves the temperature uniformity performance among a plurality of sample tubes 3.
The number of the transverse heating wires 23 can be 8, 12 or other, and the number of the longitudinal heating wires 24 can be 8 or other, which can be determined according to the use requirement.
The heater 22 may be a heating resistor.
The material of the column 11 is a heat conductive material, and may be a heat conductive metal or other heat conductive materials.
The auxiliary heating module 2 is detachably connected with the temperature control module 1, so that the auxiliary heating module 2 can be conveniently detached. When in use, the auxiliary heating module 2 can be directly placed on the surface of the temperature control module 1, and can also be fixedly connected with the temperature control module 1.
The through holes on the auxiliary heating module 2 are distributed in a matrix mode in an m×n mode, wherein m is not less than 2, and n is not less than 2. Here, m may be set to 8, 12 or other numbers, n may be set to 8 or other numbers, and may be specifically determined according to the use requirements, for example, two kinds of auxiliary heating modules 2 shown in fig. 2 and 7, and when the auxiliary heating module 2 is the case shown in fig. 7, the auxiliary heating module 2 may be installed at any position of the temperature control module 1, so as to perform auxiliary heating on the column 11 herein.
In some embodiments, as shown in fig. 3, adjacent columns 11 are connected through a carrying platform 12, the carrying platform 12 is used for supporting the auxiliary heating module 2, the height of the carrying platform 12 determines the position of the heater 22 relative to the sample tube 3, and the position of the carrying platform 12 can be changed according to actual use requirements.
The heights of the carrying platforms 12 are different, and the positions of the heaters 22 are different, so that the heaters 22 can be positioned at the upper part, the middle part, the bottom part or other parts of the column 11.
Wherein, the top of cylinder 11 is higher than the height of plummer 12, consequently forms the recess between cylinder 11 and the plummer 12, and when installing auxiliary heating module 2, auxiliary heating module 2 can block into the recess, consequently can play spacing effect. Meanwhile, the auxiliary heating module 2 is clamped in the groove, so that the heater 22 is just attached to the outer wall of the cylinder 11, and heat transfer is easier.
The weight reducing holes 121 are formed in the carrying platform 12, and the weight of the whole temperature control module 1 can be reduced through the arrangement of the weight reducing holes 121, and meanwhile, the cost of raw materials can be saved.
In some embodiments, as shown in fig. 6, the bottom of the temperature control module 1 is provided with a mounting groove 13, the mounting groove 13 is used for placing temperature sensors, and the mounting groove 13 may be provided with a plurality of temperature sensors capable of being mounted to detect the temperature inside the column 11. The temperature sensor is electrically connected with the control module, and the temperature sensor can transmit detected signals to the control module, and the temperature of the temperature control module 1 and/or the heater 22 is controlled by the control module.
The working process of the matrix type temperature control auxiliary heating device for the PCR instrument comprises the following steps:
firstly, the temperature control module 1 and the auxiliary heating module 2 are installed, then the sample tube 3 passes through a through hole 21 on the auxiliary heating module 1 and is finally embedded in a column 11 of the temperature control module 1, then the temperature control module 1 controls the column 11 to be heated to a first target temperature (about 95 ℃) so as to denature DNA, at the moment, a temperature sensor can detect the temperature inside the column 11 and transmit detected temperature signals to the control module, when the first temperature sensor senses that the internal temperature of part of the column 11 does not reach the first target temperature, the control module controls the auxiliary heating module 2 to start heating, so that the heat generated by the heater 22 is transmitted to the column 11, the temperature of the column 11 is increased to the first target temperature, the temperatures inside the columns 11 are uniform, the temperature uniformity of the sample tubes 3 at the same time is good, and finally the accuracy of detection results of the sample tube 3 is good; then the temperature control module 1 is used for cooling to a second target temperature (about 60 ℃) so that the sample to be detected in the sample tube 3 is subjected to DNA replication according to the base complementary pairing principle, and the temperature control process is repeated so as to complete replication and amplification of the sample to be detected.
The heater 22 may be activated singly or in a plurality, and the temperature sensor transmits a signal to the control module according to the signal detected by the temperature sensor, and the control module controls the corresponding transverse heating wire 23 and the corresponding longitudinal heating wire 24 to be electrified, so that the heater 22 at the intersection of the transverse heating wire 23 and the longitudinal heating wire 24 is communicated with the transverse heating wire 23 and the longitudinal heating wire 24, the heater 22 starts heating, and then heat is transmitted to the cylinder 11 around the heater 22, so that the temperature of the cylinder 11 is raised to the first target temperature.
Referring to fig. 8, an embodiment of the present disclosure provides a PCR instrument, including: the heat dissipation device 4 and the matrix type temperature control auxiliary heating device for the PCR instrument are positioned above the heat dissipation device 4, and the heaters 22 of the matrix type temperature control auxiliary heating device for the PCR instrument have different heating powers, so that the problem of poor temperature uniformity is solved.
The structure of the matrix-type temperature control auxiliary heating device for the PCR instrument in the present embodiment is the same as or similar to that described above, and will not be described in detail herein, and reference may be made to the description of the matrix-type temperature control auxiliary heating device for the PCR instrument.
In the description of the present utility model, it should be understood that the orientation or positional relationship indicated is based on the orientation or positional relationship shown in the drawings, and is merely for convenience in describing the present utility model and simplifying the description, and does not indicate or imply that the apparatus or element referred to must have a specific orientation, be configured and operated in a specific orientation, and thus should not be construed as limiting the present utility model.
In the description of the present specification, a description referring to terms "one embodiment," "some embodiments," "examples," "specific examples," or "some examples," etc., means that a particular feature, structure, material, or characteristic described in connection with the embodiment or example is included in at least one embodiment or example of the present utility model. In this specification, schematic representations of the above terms do not necessarily refer to the same embodiments or examples. Furthermore, the particular features, structures, materials, or characteristics described may be combined in any suitable manner in any one or more embodiments or examples.
Although embodiments of the present utility model have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made therein without departing from the principles and spirit of the utility model, the scope of which is defined in the appended claims and their equivalents.
Claims (10)
1. A matrix-type temperature-controlled auxiliary heating device for a PCR instrument, comprising:
the temperature control module comprises a plurality of columns, and a containing groove for placing the sample tube is formed in each column so that the temperature of the sample tube can be adjusted in the containing groove;
the auxiliary heating module is connected with the temperature control module, a plurality of through holes distributed in a matrix form are formed in the auxiliary heating module, the column penetrates through the through holes, a plurality of transverse heating wires and a plurality of longitudinal heating wires are further arranged on the auxiliary heating module, the transverse heating wires are positioned on one side of one row of the through holes, one transverse heating wire corresponds to one row of the through holes, the longitudinal heating wires are positioned on one side of one row of the through holes, one longitudinal heating wire corresponds to one row of the through holes, the transverse heating wires and the longitudinal heating wires are staggered to form intersecting points, heaters are arranged at least at part of the intersecting points, and the corresponding transverse heating wires and longitudinal heating wires at the intersecting points are electrically connected with the heaters;
the control module is respectively and electrically connected with the transverse heating wires and the longitudinal heating wires, and controls the transverse heating wires and the longitudinal heating wires to be sequentially powered on and powered off, and when the power is on, the transverse heating wires, the longitudinal heating wires and the corresponding heaters form loops so that the corresponding heaters heat the corresponding columns;
the temperature sensor is electrically connected with the control module, the temperature sensor is used for detecting the temperature inside the cylinder, the temperature sensor is used for transmitting detected signals to the control module, and the temperature of the heater is controlled by the control module.
2. The matrix-type temperature-control auxiliary heating device for a PCR instrument according to claim 1, wherein when one of the lateral heating wires is energized, the remaining lateral heating wires are not energized, a plurality of the longitudinal heating wires are sequentially energized, and only one of the longitudinal heating wires is energized at the same time, so that the heater heats the corresponding column body one by one.
3. The matrix-type temperature-controlled auxiliary heating device for a PCR instrument according to claim 1, wherein when one of the lateral heating wires is energized, the remaining lateral heating wires are not energized, and at least two of the longitudinal heating wires are energized simultaneously in groups, so that the heaters heat the corresponding column in groups.
4. A matrix-type temperature-controlled auxiliary heating apparatus for a PCR instrument according to claim 2 or 3, wherein the energizing durations of the plurality of lateral heating wires are different or the energizing durations of the plurality of longitudinal heating wires are different, so that the heating powers are different between the heaters.
5. A matrix temperature controlled auxiliary heating apparatus for a PCR instrument according to any one of claims 1-3, wherein the auxiliary heating module is detachably connected to the temperature control module.
6. A matrix temperature controlled auxiliary heating apparatus for a PCR instrument according to any one of claims 1 to 3, wherein adjacent columns are connected by a carrier for supporting the auxiliary heating module.
7. The matrix-type temperature-controlled auxiliary heating apparatus for a PCR instrument as claimed in claim 6, wherein the top of the column is higher than the height of the carrying table.
8. The matrix-type temperature-control auxiliary heating device for a PCR instrument according to claim 6, wherein the bearing table is provided with a lightening hole.
9. The matrix-type temperature-controlled auxiliary heating device for a PCR instrument according to claim 1, wherein the through holes on the auxiliary heating module are distributed in a matrix manner in a manner of m x n, wherein m is not less than 2 and n is not less than 2.
10. A PCR instrument, comprising: a heat sink and a matrix temperature controlled auxiliary heating device for a PCR instrument according to any one of claims 1 to 9, said matrix temperature controlled auxiliary heating device for a PCR instrument being located above said heat sink.
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| Application Number | Priority Date | Filing Date | Title |
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| CN202211425071.7A CN115572675A (en) | 2022-11-15 | 2022-11-15 | Matrix temperature control auxiliary heating device for PCR instrument and PCR instrument |
| CN2022114250717 | 2022-11-15 |
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| CN202211425071.7A Pending CN115572675A (en) | 2022-11-15 | 2022-11-15 | Matrix temperature control auxiliary heating device for PCR instrument and PCR instrument |
| CN202322092173.8U Active CN220550183U (en) | 2022-11-15 | 2023-08-04 | Matrix type temperature control auxiliary heating device for PCR instrument and PCR instrument |
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| JP3112183B2 (en) * | 1991-08-27 | 2000-11-27 | 東海カーボン株式会社 | Flat temperature sensor |
| JPH11287831A (en) * | 1998-04-01 | 1999-10-19 | Canon Inc | Method and device for measuring resistance, and device of electronic source and its manufacture |
| JP3478238B2 (en) * | 2000-04-28 | 2003-12-15 | 富士ゼロックス株式会社 | Array-type electronic component inspection equipment |
| CN200988331Y (en) * | 2006-11-23 | 2007-12-12 | 黄生新 | Matrix type Electric heating table for printing pattern |
| JP5545021B2 (en) * | 2010-05-14 | 2014-07-09 | トヨタ紡織株式会社 | Temperature distribution detection device, human body detection device, and vehicle seat |
| CN101974421B (en) * | 2010-10-26 | 2014-07-23 | 秦荣 | Variable-temperature metal module with precise temperature compensation and temperature compensating method |
| CN210481399U (en) * | 2019-05-30 | 2020-05-08 | 杭州安誉科技有限公司 | Auxiliary heating device for gene amplification instrument module |
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