CN220450184U - Cell supernatant negative pressure collecting bottle - Google Patents
Cell supernatant negative pressure collecting bottle Download PDFInfo
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- CN220450184U CN220450184U CN202321968940.0U CN202321968940U CN220450184U CN 220450184 U CN220450184 U CN 220450184U CN 202321968940 U CN202321968940 U CN 202321968940U CN 220450184 U CN220450184 U CN 220450184U
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- bottle
- liquid suction
- negative pressure
- bottle cap
- cap
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- 239000006228 supernatant Substances 0.000 title claims abstract description 59
- 239000007788 liquid Substances 0.000 claims abstract description 76
- 239000012528 membrane Substances 0.000 claims abstract description 13
- 238000000034 method Methods 0.000 claims abstract description 8
- 230000008569 process Effects 0.000 claims abstract description 7
- 238000001914 filtration Methods 0.000 claims abstract description 6
- 238000003860 storage Methods 0.000 claims description 8
- 239000011148 porous material Substances 0.000 claims description 6
- 238000005213 imbibition Methods 0.000 claims 1
- 238000004321 preservation Methods 0.000 abstract description 9
- 210000004027 cell Anatomy 0.000 description 25
- 230000008901 benefit Effects 0.000 description 6
- 238000004113 cell culture Methods 0.000 description 6
- 239000000463 material Substances 0.000 description 3
- 238000012986 modification Methods 0.000 description 3
- 230000004048 modification Effects 0.000 description 3
- 230000009471 action Effects 0.000 description 2
- 239000010788 consumable waste Substances 0.000 description 2
- 238000001514 detection method Methods 0.000 description 2
- 238000009826 distribution Methods 0.000 description 2
- 239000003344 environmental pollutant Substances 0.000 description 2
- 210000001808 exosome Anatomy 0.000 description 2
- 238000000605 extraction Methods 0.000 description 2
- 231100000719 pollutant Toxicity 0.000 description 2
- 241000894006 Bacteria Species 0.000 description 1
- 241001391944 Commicarpus scandens Species 0.000 description 1
- 230000004888 barrier function Effects 0.000 description 1
- 239000000919 ceramic Substances 0.000 description 1
- 238000004140 cleaning Methods 0.000 description 1
- 238000010276 construction Methods 0.000 description 1
- 238000011109 contamination Methods 0.000 description 1
- 239000012228 culture supernatant Substances 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 239000011152 fibreglass Substances 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 230000005484 gravity Effects 0.000 description 1
- 239000003102 growth factor Substances 0.000 description 1
- 238000001802 infusion Methods 0.000 description 1
- 238000002955 isolation Methods 0.000 description 1
- 239000004033 plastic Substances 0.000 description 1
- 229920000642 polymer Polymers 0.000 description 1
- 238000003825 pressing Methods 0.000 description 1
- 239000000047 product Substances 0.000 description 1
- 238000005057 refrigeration Methods 0.000 description 1
- 238000007789 sealing Methods 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 210000000130 stem cell Anatomy 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 239000013589 supplement Substances 0.000 description 1
- 210000001519 tissue Anatomy 0.000 description 1
Landscapes
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The utility model provides a cell supernatant negative pressure collecting bottle, which comprises a bottle body, a collecting bottle cap, a preserving bottle cap, a liquid suction hose, a rubber cap and a one-way filtering piece, wherein: the collecting bottle cap can be detachably arranged above the opening of the bottle body, wherein the collecting bottle cap is provided with a liquid suction port and an air exhaust port, and the interior of the bottle body is in a negative pressure state in the liquid suction process; the preservation bottle cap can be detachably arranged above the opening of the bottle body in a way of replacing the collection bottle cap, so that a closed space is formed between the preservation bottle cap and the bottle body; the first end of the liquid suction hose is connected with the liquid suction port, and the second end of the liquid suction hose is used for sucking the supernatant; the rubber cap is detachably arranged above the exhaust port; the unidirectional filter spare is located the inboard of gas vent, and unidirectional filter spare includes unidirectional filter spare intake duct, disc structure and unidirectional filter spare air outlet channel, and disc structure inside has unidirectional filter membrane, and disc structure is fixed at the top of collecting bottle lid. The utility model can safely, conveniently, efficiently and massively collect the cell supernatant.
Description
Technical Field
The utility model relates to the technical field of biomedical experimental instruments, in particular to a cell supernatant negative pressure collecting bottle.
Background
Cell culture is a common experimental means in biomedical science, and the culture product includes cell tissue mass and supernatant. The supernatant can reflect the cell state, and the components such as various growth factors and exosomes contained in the supernatant (especially the supernatant of stem cell culture) can play various roles in medical treatment and cosmetology. This often requires isolation and extraction from the supernatant of a large number of cell cultures followed by concentration.
The traditional way to collect cell supernatant is to aspirate the supernatant in a culture vessel using a pipette, and then move the supernatant into a collection tube or bottle. Such an operation needs to be performed in an open manner in a clean bench, and the cover of the culture container and the cover of the collection tube or bottle need to be repeatedly opened, which is liable to cause contamination. Moreover, due to the limited range of pipettes, the pipettes need to be replaced frequently, and consumable materials of the pipettes are wasted.
Disclosure of Invention
In view of the above, the present utility model provides a negative pressure cell supernatant collection bottle capable of safely, conveniently, efficiently and massively collecting supernatant.
The utility model provides a cell supernatant negative pressure collecting bottle, which comprises a bottle body, a collecting bottle cap, a preserving bottle cap, a liquid suction hose, a rubber cap and a one-way filtering piece, wherein: the collecting bottle cap can be detachably arranged above the opening of the bottle body, wherein the collecting bottle cap is provided with a liquid suction opening and an air exhaust opening, and the interior of the bottle body is in a negative pressure state in the liquid suction process; the storage bottle cap can be detachably arranged above the opening of the bottle body in a way of replacing the collecting bottle cap, so that a closed space is formed by the storage bottle cap and the bottle body; the first end of the liquid suction hose is connected with the liquid suction port, and the second end of the liquid suction hose is used for sucking supernatant; the rubber cap is detachably arranged above the exhaust port; the unidirectional filter spare is located the inboard of gas vent, unidirectional filter spare includes unidirectional filter spare intake duct, disc structure and unidirectional filter spare air outlet channel, the disc structure is inside to have unidirectional filter membrane, the disc structure is fixed the top of collecting bottle lid.
Optionally, the device further comprises a liquid suction diversion short pipe, wherein the liquid suction diversion short pipe is arranged between the liquid suction hose and the liquid suction port, and part of the liquid suction diversion short pipe is positioned in the closed space.
Optionally, the method further comprises: and the flow rate control device is arranged on the middle section of the liquid suction hose.
Optionally, the liquid suction port is located at the side of the collecting bottle cap, and the air exhaust port is located at the top of the collecting bottle cap; or, the liquid suction port is positioned at the top of the collecting bottle cap, and the air exhaust port is positioned at the side of the collecting bottle cap.
Optionally, the unidirectional filtration membrane is a unidirectional filtration membrane having a pore size of 0.22 microns.
Optionally, a unidirectionally moveable valve is also included, the unidirectionally moveable valve being located within the liquid intake.
Optionally, the bottle body has a scale.
Optionally, the bottle body is provided with a holding part which is convenient for a user to hold.
Optionally, the second end of the pipette hose is provided with a tapered suction head.
The cell supernatant negative pressure collecting bottle provided by the utility model has the advantages that the inside of the bottle body is in a negative pressure state in the working process, so that under the action of the external atmospheric pressure, the supernatant in the culture container flows into the bottle body from the liquid suction hose, and the cell supernatant negative pressure collecting bottle in the embodiment of the utility model has at least the following advantages:
(1) The cover of the collecting bottle does not need to be repeatedly opened, the cell culture container also needs to be opened once, the operation is simple and efficient, and the pollution caused by repeated uncovering is avoided;
(2) A pipette is not required, so that consumable waste is avoided;
(3) Transferring the collected supernatant without using a pipette, without dropping the supernatant, without wasting the collected supernatant, and keeping the operation table clean;
(4) The bottle body can be a large-volume container, and can collect the supernatant efficiently and on a large scale.
Drawings
The drawings are included to provide a better understanding of the utility model and are not to be construed as unduly limiting the utility model. Wherein:
FIG. 1 is a general schematic of a negative pressure cell supernatant collection bottle according to an embodiment of the present utility model;
FIG. 2 is an enlarged view of a portion of the body, collection bottle cap and rubber cap of FIG. 1;
FIG. 3 is a schematic cross-sectional view of the body and collection bottle cap of FIG. 1;
FIG. 4 is a schematic perspective view of a preservation bottle cap in a negative pressure cell supernatant collection bottle according to an embodiment of the present utility model;
FIG. 5 is a schematic perspective view of a one-way filter in a negative pressure cell supernatant collection bottle according to an embodiment of the present utility model.
Wherein: 1. a bottle body; 2. collecting bottle caps; 3. preserving the bottle cap; 4. a scale; 5. a grip portion; 6. an exhaust port; 7. a rubber cap; 8. a first end of the liquid suction hose; 81. a sharp portion; 82. a through hole; 9. a liquid suction port; 10. a liquid suction hose; 11. a one-way filter; 11a, a one-way filter inlet channel; 11b, a disc structure; 11c, one-way filter outlet channels; 12. a liquid suction diversion short pipe; 13. conical suction head; 14. and a flow rate control device.
Detailed Description
Exemplary embodiments of the present utility model will now be described with reference to the accompanying drawings, in which various details of embodiments of the present utility model are included to facilitate understanding, and are to be considered merely exemplary. Accordingly, those of ordinary skill in the art will recognize that various changes and modifications of the embodiments described herein can be made without departing from the scope and spirit of the utility model. Descriptions of well-known functions and constructions are omitted in the following description for clarity and conciseness.
FIG. 1 is a schematic view of a negative pressure cell supernatant collection bottle (the preservation bottle cap 3 is omitted from drawing) according to an embodiment of the present utility model; FIG. 2 is an enlarged view of a portion of the body, collection bottle cap and rubber cap of FIG. 1; fig. 3 is a schematic cross-sectional view of the body and collection bottle cap of fig. 1.
As shown in fig. 1, the liquid collection container of the cell supernatant negative pressure collection bottle mainly comprises a bottle body 1, a collection bottle cap 2, a preservation bottle cap 3, a liquid suction hose 10, a rubber cap 7 and a one-way filter 11.
The body 1 is generally cylindrical, but this is for example only. In other embodiments, the shape may be a substantially square column. The bottle body 1 is made of sterile plastic, and has the advantage of being not easy to break compared with the traditional glass material. Preferably, the body 1 has a scale 4 to facilitate the user's reading of the volume of supernatant collected. The bottle body 1 can also be provided with a holding part 5, so that the bottle body is convenient to hold and is not easy to slide off. The holding part 5 can be a groove with an arc-shaped or fold-line-shaped section around the circumference of the bottle body, and can also be a handle or a lifting ring.
The collecting bottle cap 2 can be detachably disposed over the opening of the bottle body 1. For example, the collecting bottle cap 2 is connected with the opening of the bottle body 1 by screw threads. The collection bottle cap 2 has a liquid suction port 9 and a gas discharge port 6. As shown in fig. 2, the liquid suction port 9 is located at the side of the collection bottle cap 2, and the air discharge port 6 is located at the top of the collection bottle cap 2. In other embodiments of the present utility model, the liquid suction port 9 may be located at the top of the collection bottle cap 2, and the air discharge port 6 may be located at the side of the collection bottle cap 2.
The storage bottle cap 3 can be detachably arranged above the opening of the bottle body 1 in place of the collecting bottle cap 2, so that the storage bottle cap 3 and the bottle body 1 form a closed space. The collecting bottle cap 2 and the preserving bottle cap 3 are not simultaneously mounted on the bottle body 1, but alternatively are used. In particular, the holding cap 3 is adapted to the shape and size of the opening of the body 1 and can be detachably connected, for example by means of a screw connection. As shown in fig. 4, the preservation bottle cap 3 is cylindrical, and the side wall is provided with vertical line anti-slip lines, so that a user can apply force to twist. After the collecting bottle cap 2 is matched with the bottle body 1 to complete the collecting function, the collecting bottle cap 2 is replaced by the preservation bottle cap 3 to seal the bottle body 1, and then the whole cell supernatant negative pressure collecting bottle is put in a refrigerator for temporary storage or directly sent to a detection place.
The first end of the liquid suction hose 10 is connected with the liquid suction port 9, and the second end of the liquid suction hose 10 is used for sucking the supernatant liquid. A rubber cap 7 is detachably provided over the exhaust port 6.
A rubber cap 7 is detachably provided over the exhaust port 6. When the rubber cap 7 is detached, an external negative pressure device can be connected with the air outlet 6 to supplement the negative pressure in the bottle.
The cell supernatant negative pressure collecting bottle of the embodiment of the utility model is a pretreated negative pressure bottle, and a rubber cap 7 is blocked at the position of the air outlet 6 to help the bottle body 1 to maintain negative pressure. In the process of collecting liquid, the rubber cap 7 is not required to be opened initially, and in the use process, if the atmospheric pressure is insufficient, the rubber cap 7 can be detached and then an external negative pressure device is connected to assist in maintaining the negative pressure environment in the bottle.
The liquid suction port 9 preferably incorporates a movable one-way valve (not shown) which allows liquid to flow into the bottle but not back, and is externally closed to maintain a vacuum environment within the bottle. In the case of a one-way valve, the first end 8 of the liquid suction hose can be provided with a sharp part 81 and a through hole 82 (similar to the connection part of the liquid suction hose and the infusion bottle) is arranged on the front end surface, so that the first end 8 of the liquid suction hose can easily break through the sealing part on the inner side of the liquid suction port 9, and can allow liquid to pass through.
The cell supernatant negative pressure collecting bottle can further comprise a liquid suction guide short pipe 12, wherein the liquid suction guide short pipe 12 is arranged on the inner side of the liquid suction opening 9 (the inner side is one side closer to the central position of the bottle body), and part of the liquid suction guide short pipe 12 is positioned in a closed space in the bottle. The provision of the liquid suction and guide short tube 12 can enable the sucked supernatant liquid to drop into the bottle body 1 more stably, but not flow down along the inner wall of the bottle body 1 due to gravity, so that the possibility that the supernatant liquid is stained with the other parts of the collecting bottle cap 2 is reduced, and the cleaning is facilitated.
The one-way filter 11 in the cell supernatant negative pressure collecting bottle is located inside the exhaust port 6 (inside means the side closer to the center of the bottle body). The unidirectional filter 11 includes a unidirectional filter inlet channel 11a, a disc structure 11b, and a unidirectional filter outlet channel 11c. The disc structure 11b has a unidirectional filter membrane inside, and the disc structure 11b is fixed on top of the collecting bottle cap 2. As shown in fig. 5, the disc structure 11b has a unidirectional filter membrane with a pore size of 0.22um inside, the unidirectional filter inlet channel 11a is provided below the disc structure 11b, and the unidirectional filter outlet channel 11c is provided above the disc structure 11b. The unidirectional filter 11 is fixed inside the top of the collecting bottle cap 2, and specifically, a rubber binding with a specific shape is arranged inside the top of the collecting bottle cap 2, and the rubber binding can be provided with a groove or rubber threads so as to be matched with the disc structure 11b of the unidirectional filter.
Through setting up one-way filter 11, can let the inside of body 1 and external negative pressure equipment between form a barrier, gaseous can only follow in the bottle to external negative pressure equipment, if remain in the external negative pressure equipment pollutants such as bacterium and then can't retrograde entering bottle to can effectively avoid the pollutant to fall into the bottle.
The unidirectional filter 11 is typically made of a material such as a polymer, fiberglass or ceramic. The pore size, shape, distribution, etc. of the unidirectional filter 11 can be controlled by a specific process. The most common is a breathable unidirectional filter, and the principle of the breathable unidirectional filter is that the size and the distribution mode of gas molecules are utilized, so that the gas molecules can freely diffuse through the pores of the membrane on one side of the membrane, and the gas molecules cannot pass through the pores of the membrane on the other side of the membrane, thereby realizing the unidirectional permeation effect of the gas. Preferably, a 0.22 micron sized unidirectional filter 11 is used which has the advantage of being technically mature, versatile and readily available.
In order to better aspirate the cell supernatant, the second end of the pipetting hose 10 is provided with a conical tip 13. The conical tip 13 can be used directly with a conventional pipette tip with a small tip opening so that it can be reached into the corner of the container holding the liquid to be aspirated, thereby collecting as much of the cell supernatant as possible.
Before use, the cell supernatant negative pressure collecting bottle is checked whether all parts are tightly connected and airtight. The first end of the liquid sucking hose 10 is inserted into the liquid sucking port 9, and the inner seal is pierced so that the liquid sucking hose 10 is communicated with the liquid sucking port 9. The flow rate control device 14 is adjusted to set the liquid flow rate to 0. The second end of the mounted liquid suction hose 10 is extended below the liquid level of the cells to be sucked, the flow rate control device 14 is gently adjusted to make the flow rate of the liquid suitable, and the cell culture supernatant is sucked by utilizing the negative pressure in the bottle body 1. After the collection, the collection bottle cap 2 and the parts such as the liquid suction hose 10 connected with the collection bottle cap are removed and replaced by the storage bottle cap 3. The bottle body 1 and the preservation bottle cap 3 are put into a refrigeration environment with the temperature of 4 ℃ for temporary preservation or sent to the next stage for separation and extraction or biochemical detection of active factors or exosomes.
The cell supernatant negative pressure collection bottle can further comprise a flow rate control device 14 arranged on the middle section of the liquid suction hose 10, and the flow rate control device 14 can adjust the flow cross section area to finally control the flow rate of liquid by adjusting the degree of pressing the liquid suction hose 10, so that the vacuum environment in the bottle can be maintained conveniently.
The cell supernatant negative pressure collecting bottle provided by the utility model has the advantages that the inside of the bottle body is in a negative pressure environment, so that under the action of the external atmospheric pressure, the supernatant in the culture container flows into the bottle from the liquid suction hose, and the cell supernatant negative pressure collecting bottle provided by the embodiment of the utility model has at least the following advantages:
(1) The cover of the collecting bottle does not need to be repeatedly opened, the cell culture container also needs to be opened once, the operation is simple and efficient, and the pollution caused by repeated uncovering is avoided;
(2) A pipette is not required, so that consumable waste is avoided;
(3) Transferring the collected supernatant without using a pipette, without dropping the supernatant, without wasting the collected supernatant, and keeping the operation table clean;
(4) The bottle body can be a large-volume container, and can collect the supernatant efficiently and on a large scale.
The above embodiments do not limit the scope of the present utility model. It will be apparent to those skilled in the art that various modifications, combinations, sub-combinations and alternatives can occur depending upon design requirements and other factors. Any modifications, equivalent substitutions and improvements made within the spirit and principles of the present utility model should be included in the scope of the present utility model.
Claims (9)
1. The utility model provides a cell supernatant negative pressure collecting bottle which characterized in that, includes body, collecting bottle lid, preserve bottle lid, imbibition hose, rubber cap and one-way filter, wherein:
the collecting bottle cap can be detachably arranged above the opening of the bottle body, wherein the collecting bottle cap is provided with a liquid suction opening and an air exhaust opening, and the interior of the bottle body is in a negative pressure state in the liquid suction process;
the storage bottle cap can be detachably arranged above the opening of the bottle body in a way of replacing the collecting bottle cap, so that a closed space is formed by the storage bottle cap and the bottle body;
the first end of the liquid suction hose is connected with the liquid suction port, and the second end of the liquid suction hose is used for sucking supernatant;
the rubber cap is detachably arranged above the exhaust port;
the unidirectional filter spare is located the inboard of gas vent, unidirectional filter spare includes unidirectional filter spare intake duct, disc structure and unidirectional filter spare air outlet channel, the disc structure is inside to have unidirectional filter membrane, the disc structure is fixed the top of collecting bottle lid.
2. The negative pressure cell supernatant collection bottle of claim 1, further comprising a liquid suction flow conduit disposed between the liquid suction hose and the liquid suction port, a portion of the liquid suction flow conduit being located within the enclosed space.
3. The negative pressure cell supernatant collection bottle of claim 1, further comprising: and the flow rate control device is arranged on the middle section of the liquid suction hose.
4. The negative pressure cell supernatant collection bottle of claim 1, wherein the liquid suction port is located laterally of the collection bottle cap and the gas discharge port is located at the top of the collection bottle cap; or, the liquid suction port is positioned at the top of the collecting bottle cap, and the air exhaust port is positioned at the side of the collecting bottle cap.
5. The negative pressure cell supernatant collection bottle of claim 1, wherein the one-way filtration membrane is a one-way filtration membrane having a pore size of 0.22 microns.
6. The negative pressure cell supernatant collection bottle of claim 1, further comprising a one-way movable valve positioned within the pipetting opening.
7. The negative pressure cell supernatant collection bottle of any one of claims 1 to 6 wherein the body has graduations.
8. The negative pressure cell supernatant collection bottle of any one of claims 1 to 6 wherein the body has a grip portion for convenient gripping by a user.
9. The negative pressure cell supernatant collection bottle of any one of claims 1 to 6 wherein the second end of the pipetting hose is provided with a tapered tip.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202321968940.0U CN220450184U (en) | 2023-07-25 | 2023-07-25 | Cell supernatant negative pressure collecting bottle |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202321968940.0U CN220450184U (en) | 2023-07-25 | 2023-07-25 | Cell supernatant negative pressure collecting bottle |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN220450184U true CN220450184U (en) | 2024-02-06 |
Family
ID=89733579
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202321968940.0U Active CN220450184U (en) | 2023-07-25 | 2023-07-25 | Cell supernatant negative pressure collecting bottle |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN220450184U (en) |
-
2023
- 2023-07-25 CN CN202321968940.0U patent/CN220450184U/en active Active
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