CN220425231U - Mixing stirring device for antibody diluent - Google Patents
Mixing stirring device for antibody diluent Download PDFInfo
- Publication number
- CN220425231U CN220425231U CN202321433479.9U CN202321433479U CN220425231U CN 220425231 U CN220425231 U CN 220425231U CN 202321433479 U CN202321433479 U CN 202321433479U CN 220425231 U CN220425231 U CN 220425231U
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- mixing
- stirring device
- mixing tank
- stirring
- piece
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- 238000002156 mixing Methods 0.000 title claims abstract description 93
- 238000003756 stirring Methods 0.000 title claims abstract description 66
- 239000003085 diluting agent Substances 0.000 title claims abstract description 34
- 238000013016 damping Methods 0.000 claims abstract description 27
- 230000005540 biological transmission Effects 0.000 claims description 15
- 238000007789 sealing Methods 0.000 claims description 7
- 239000011550 stock solution Substances 0.000 abstract description 29
- 230000032683 aging Effects 0.000 abstract description 7
- 230000000694 effects Effects 0.000 abstract description 7
- 230000000903 blocking effect Effects 0.000 abstract description 4
- 238000010790 dilution Methods 0.000 abstract description 3
- 239000012895 dilution Substances 0.000 abstract description 3
- 230000009467 reduction Effects 0.000 abstract description 2
- 239000007788 liquid Substances 0.000 description 9
- 230000003139 buffering effect Effects 0.000 description 7
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 6
- 239000000463 material Substances 0.000 description 4
- 238000000034 method Methods 0.000 description 3
- 230000008569 process Effects 0.000 description 3
- 239000002994 raw material Substances 0.000 description 3
- 108091003079 Bovine Serum Albumin Proteins 0.000 description 2
- 229940098773 bovine serum albumin Drugs 0.000 description 2
- 235000018102 proteins Nutrition 0.000 description 2
- 108090000623 proteins and genes Proteins 0.000 description 2
- 102000004169 proteins and genes Human genes 0.000 description 2
- 108010010803 Gelatin Proteins 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 239000001888 Peptone Substances 0.000 description 1
- 108010080698 Peptones Proteins 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 230000004075 alteration Effects 0.000 description 1
- 239000000427 antigen Substances 0.000 description 1
- 108091007433 antigens Proteins 0.000 description 1
- 102000036639 antigens Human genes 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 239000005018 casein Substances 0.000 description 1
- BECPQYXYKAMYBN-UHFFFAOYSA-N casein, tech. Chemical compound NCCCCC(C(O)=O)N=C(O)C(CC(O)=O)N=C(O)C(CCC(O)=N)N=C(O)C(CC(C)C)N=C(O)C(CCC(O)=O)N=C(O)C(CC(O)=O)N=C(O)C(CCC(O)=O)N=C(O)C(C(C)O)N=C(O)C(CCC(O)=N)N=C(O)C(CCC(O)=N)N=C(O)C(CCC(O)=N)N=C(O)C(CCC(O)=O)N=C(O)C(CCC(O)=O)N=C(O)C(COP(O)(O)=O)N=C(O)C(CCC(O)=N)N=C(O)C(N)CC1=CC=CC=C1 BECPQYXYKAMYBN-UHFFFAOYSA-N 0.000 description 1
- 235000021240 caseins Nutrition 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 239000012530 fluid Substances 0.000 description 1
- 229920000159 gelatin Polymers 0.000 description 1
- 239000008273 gelatin Substances 0.000 description 1
- 235000019322 gelatine Nutrition 0.000 description 1
- 235000011852 gelatine desserts Nutrition 0.000 description 1
- 238000003119 immunoblot Methods 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 235000019319 peptone Nutrition 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 230000001681 protective effect Effects 0.000 description 1
- 210000002966 serum Anatomy 0.000 description 1
- 235000020183 skimmed milk Nutrition 0.000 description 1
- 230000000638 stimulation Effects 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
Landscapes
- Mixers With Rotating Receptacles And Mixers With Vibration Mechanisms (AREA)
Abstract
The utility model discloses a mixing and stirring device for an antibody diluent, which relates to the technical field of antibody dilution and stirring and comprises a mixing tank, wherein a stirring assembly for stirring and mixing stock solution in the mixing tank is arranged in the mixing tank, a feed pipe and an electromagnetic valve are respectively arranged on two sides of the mixing tank, two first springs and a first damping cylinder are arranged in the mixing tank, a blocking piece is arranged at the other ends of the first springs and the first damping cylinder, the blocking piece abuts against one end of the feed pipe, and a sliding piece is arranged in the mixing tank in a sliding manner. According to the utility model, the mixing and stirring device of the antibody diluent is convenient to use, has more functions, can buffer the stock solution injected into the mixing tank, not only improves the stability of the mixing and stirring device, but also can help to slow down the ageing and damage rate of equipment, and simultaneously has a certain noise reduction effect.
Description
Technical Field
The utility model relates to the technical field of antibody dilution stirring, in particular to a mixing and stirring device for an antibody diluent.
Background
The antibody is protein with protective effect generated by the stimulation of antigen, the antibody diluent is used for dilution and preparation of primary antibody and secondary antibody in immunoblotting detection, the body diluent is usually prepared from protein such as skimmed milk, bovine Serum Albumin (BSA), serum, casein, animal gelatin, plant peptone and the like, and a mixing and stirring device is needed for preparing the antibody diluent.
However, most of the prior mixing and stirring devices are not provided with a liquid buffer structure, and the pump body is usually used for conveying raw material liquid when the raw material liquid is introduced into the mixing and stirring device, but the impact force of the liquid under the action of the water pump is large when the water pump pumps the raw material liquid into the equipment, so that the stability of the mixing and stirring device is reduced, larger noise is generated, and the equipment aging and damage probability can be accelerated even for a long time.
For example, the prior Chinese patent (publication No. CN 212283821U) discloses a mixing stirring device for a diluent, a driving device is adopted to drive a connecting shaft in a stirring container to lift, and then a material lifting assembly in the stirring container is driven to lift, and in the lifting process of the connecting shaft and the stirring container, the material lifting assembly can roll materials in the stirring container and realize lifting of partial materials.
The mixing and stirring device of the diluent is only provided with a stirring structure in the mixing and stirring device, but the stirring structure is an essential structure of the existing mixing and stirring device, and the mixing and stirring device of the diluent is not provided with any structure for buffering liquid in the mixing and stirring device of the diluent, so that the liquid conveyed by the water pump has larger impact force, and the stability of equipment and the probability of ageing and damage of the equipment are reduced.
Disclosure of Invention
The utility model provides a mixing and stirring device for an antibody diluent, which solves the technical problem that most of the existing mixing and stirring devices for the antibody diluent cannot buffer a stock solution entering equipment.
In order to solve the technical problems, the mixing and stirring device for the antibody diluent comprises a mixing tank, wherein a stirring assembly is arranged in the mixing tank and used for stirring and mixing stock solution in the mixing tank, a feeding pipe and an electromagnetic valve are respectively arranged on two sides of the mixing tank, two first springs and a first damping cylinder are symmetrically arranged in the mixing tank, a blocking piece is arranged at the other ends of the first springs and the first damping cylinder and abuts against one end of the feeding pipe, and the first springs are movably sleeved on the first damping cylinder.
Preferably, the mixing tank is provided with a sliding part in a sliding manner, a buffer part is arranged below the sliding part, the buffer part comprises two sliding blocks, a second spring and a second damping cylinder, a sliding rail is arranged at the bottom of the sliding part, the sliding blocks are connected in the sliding rail in a sliding manner, two ends of the second spring and two ends of the second damping cylinder are respectively fixedly connected with the sliding blocks and the inner wall of the sliding rail, and the sliding blocks are connected with the inner bottom wall of the mixing tank.
Preferably, the bottom of slider is provided with the mount pad, be provided with two fixing bases in the blending tank, the mount pad rotation is provided with the commentaries on classics piece, the other end of commentaries on classics piece rotates the setting in the fixing base.
Preferably, a first pin shaft is arranged in the mounting seat, a second pin shaft is arranged in the fixing seat, and two ends of the rotating piece are respectively and rotatably sleeved on the first pin shaft and the second pin shaft.
Preferably, the stirring assembly comprises a fixed plate, a motor, a transmission assembly, two rotating shafts and a plurality of stirring rods, wherein the fixed plate is arranged in the mixing tank, the rotating shafts are rotationally connected to the bottom of the fixed plate, the stirring rods are arranged on the rotating shafts, the motor is arranged at the top of the fixed plate, a driving shaft of the motor is in transmission connection with one end of one rotating shaft, and the transmission assembly is connected with the rotating shafts.
Preferably, the transmission assembly comprises a transmission chain and two chain wheels, wherein the chain wheels are fixedly sleeved on the rotating shaft, and the transmission chain is connected to the two chain wheels in a meshed mode.
Preferably, the bottom of the mixing tank is provided with a plurality of underframes.
Preferably, sealing strips are arranged on the periphery of the sliding piece and are propped against the inner wall of the mixing tank, and the sealing strips are made of rubber.
Compared with the related art, the mixing and stirring device for the antibody diluent has the following beneficial effects:
1. according to the utility model, after the stock solution is conveyed into the feeding pipe by the water pump, the stock solution firstly collides on the baffle member, so that the baffle member stretches the first spring to stretch, and when the stock solution stops conveying, the first spring is matched with the first damping cylinder to rebound slowly, so that the effect of buffering the conveying stock solution can be realized, the stability of the mixing and stirring device is improved, and the aging and damage rate of equipment is slowed down.
2. According to the utility model, when the stock solution enters the mixing tank and falls down, the sliding piece can slide downwards at first, the two sliding blocks are mutually separated and compress the second spring in the process, and when the stock solution stops falling down, the second spring is slowly rebounded by matching with the second damping cylinder, so that the effect of buffering the stock solution can be further realized, the stability of the equipment is further improved, and the device has a certain noise reduction function.
Drawings
FIG. 1 is a schematic perspective view of the exterior of a mixing and stirring device for antibody diluents;
FIG. 2 is a schematic plan view of the inside of a mixing and stirring device for antibody diluents;
FIG. 3 is an enlarged view of FIG. 2 at A;
fig. 4 is an enlarged view at B in fig. 2.
Reference numerals in the drawings: 1. a mixing tank; 2. a feed pipe; 3. an electromagnetic valve; 4. a first spring; 5. a first damping cylinder; 6. a blocking member; 7. a slider; 8. a slide block; 9. a mounting base; 10. a fixing seat; 11. a rotating member; 12. a second spring; 13. a second damping cylinder; 14. a fixing plate; 15. a rotating shaft; 16. a stirring rod; 17. a motor; 18. a sprocket; 19. a drive chain; 20. a chassis.
Detailed Description
Embodiments of the present utility model are described in detail below.
Examples of the embodiments are illustrated in the accompanying drawings, wherein like or similar reference numerals refer to like or similar elements or elements having like or similar functionality throughout. The embodiments described below by referring to the drawings are exemplary only for explaining the present utility model and are not to be construed as limiting the present utility model.
Example 1
The mixing and stirring device for the antibody diluent comprises a mixing tank 1, wherein a stirring assembly is arranged in the mixing tank 1 and used for stirring and mixing raw liquid in the mixing tank 1, a feed pipe 2 and an electromagnetic valve 3 are respectively arranged on two sides of the mixing tank 1, two first springs 4 and a first damping cylinder 5 are symmetrically arranged in the mixing tank 1, a baffle piece 6 is arranged at the other ends of the first springs 4 and the first damping cylinder 5, the baffle piece 6 is propped against one end of the feed pipe 2, and the first springs 4 are movably sleeved on the first damping cylinder 5;
the stirring subassembly includes fixed plate 14, motor 17, drive assembly, two pivots 15 and a plurality of puddler 16, fixed plate 14 sets up in mixing tank 1, pivot 15 rotates the bottom of connecting at fixed plate 14, puddler 16 sets up in pivot 15, motor 17 sets up at the top of fixed plate 14, and motor 17's drive shaft is connected with one end transmission of one of them pivot 15, drive assembly is connected with pivot 15, drive assembly includes drive chain 19 and two sprocket 18, sprocket 18 is fixed cup joints in pivot 15, drive chain 19 meshing is connected on two sprocket 18.
During specific implementation, the stock solution of the antibody diluent is firstly conveyed into the feed pipe 2 by using an external water pump, then the stock solution is impacted on the baffle member 6, the baffle member 6 stretches the first spring 4 to stretch, when the stock solution stops conveying, the first spring 4 is matched with the first damping cylinder 5 to enable the first spring 4 to rebound slowly, the effect of buffering the conveying stock solution can be achieved, stability of the mixing stirring device is improved, the ageing and damage rate of equipment can be slowed down, then the motor 17 is started, the transmission chain 19 drives the two chain wheels 18 and the rotating shaft 15 to rotate, and the stirring rods 16 move to stir and mix the stock solution, so that the antibody diluent can be prepared.
The bottom of the mixing tank 1 is provided with a plurality of underframes 20. The mixing and stirring device can be elevated, so that people can conveniently take the antibody diluent.
For the electromagnetic valve 3, the flow direction of the valve is perpendicular to the fluid direction, and the electromagnetic valve can only be fully opened and fully closed, and the principle is that: when the electromagnetic valve is electrified, the electromagnetic force is generated by the electromagnetic valve coil, the closing piece is lifted from the valve seat, when the valve is opened and the power is cut off, the electromagnetic force disappears, the elastic force presses the closing piece on the valve seat, and the valve is closed.
Example two
The mixing and stirring device for the antibody diluent comprises a mixing tank 1, wherein a stirring assembly is arranged in the mixing tank 1 and used for stirring and mixing raw liquid in the mixing tank 1, a feed pipe 2 and an electromagnetic valve 3 are respectively arranged on two sides of the mixing tank 1, two first springs 4 and a first damping cylinder 5 are symmetrically arranged in the mixing tank 1, a baffle piece 6 is arranged at the other ends of the first springs 4 and the first damping cylinder 5, the baffle piece 6 is propped against one end of the feed pipe 2, and the first springs 4 are movably sleeved on the first damping cylinder 5;
the inner sliding of the mixing tank 1 is provided with a sliding part 7, the buffer part is arranged below the sliding part 7 and comprises two sliding blocks 8, a second spring 12 and a second damping cylinder 13, the bottom of the sliding part 7 is provided with a sliding rail, the sliding blocks 8 are in sliding connection with the sliding rail, two ends of the second spring 12 and the second damping cylinder 13 are respectively fixedly connected with the sliding blocks 8 and the inner wall of the sliding rail, the sliding blocks 8 are connected with the inner bottom wall of the mixing tank 1, the bottom of the sliding blocks 8 is provided with a mounting seat 9, two fixing seats 10 are arranged in the mixing tank 1, a rotating part 11 is rotationally arranged in the fixing seats 10, a first pin shaft is arranged in the mounting seat 9, a second pin shaft is arranged in the fixing seats 10, and two ends of the rotating part 11 are rotationally sleeved on the first pin shaft and the second pin shaft respectively.
In particular, during implementation, according to the principle in the first embodiment, the buffer treatment can be performed when the stock solution enters the mixing tank 1, which not only helps to improve the stability of the mixing and stirring device, but also helps to slow down the aging and damage rate of the equipment, in addition, after the stock solution enters the mixing tank 1 and falls down, the stock solution can strike the sliding piece 7, so that the sliding piece 7 slides downwards, at this time, the two sliding blocks 8 can be mutually separated and compress the second spring 12, and when the stock solution stops falling down, the second spring 12 can rebound slowly in cooperation with the second damping cylinder 13, so that the effect of buffering the stock solution can be further realized.
Sealing strips are arranged on the periphery of the sliding piece 7 and are propped against the inner wall of the mixing tank 1, and the sealing strips are made of rubber. Because the rubber has certain elasticity, the sealing strip can fill the gap between the sliding piece 7 and the inner wall of the mixing tank 1, and the tightness is improved.
In order to facilitate the control of the switch of the motor 17 by personnel, a controller not shown in the figure is arranged at the rear side of the mixing tank 1, so that the switch of the motor 17 can be conveniently operated by personnel.
For the controller, by operating the controller, the controller can send an electrical signal to the motor 17 and activate it, thereby achieving the purpose of conveniently controlling the motor 17, and since the controller is the prior art, it is not described in the present specification too much.
Working principle:
when the device is used, the stock solution of the antibody diluent is firstly conveyed into the feed pipe 2 by using an external water pump, then the stock solution is impacted on the baffle 6, the baffle 6 stretches the first spring 4 to stretch, when the stock solution stops conveying, the first spring 4 is matched with the first damping cylinder 5 to rebound slowly, the effect of buffering the conveying stock solution can be realized, the stability of the mixing stirring device can be improved, the aging and damage rate of the device can be slowed down, in the process, the stock solution falls in the mixing tank 1 through the feed pipe 2 and impacts the slide 7, the slide 7 slides downwards, at the moment, the two slide blocks 8 are mutually far away and compress the second spring 12, when the stock solution stops falling, the second damping cylinder 13 is matched with the second spring 12 to rebound slowly, the effect of buffering the stock solution can be further realized, then the motor 17 is started, the transmission chain 19 is driven to rotate the two chain wheels 18 and the rotating shaft 15, the plurality of stirring rods 16 are moved to mix the stock solution, the antibody diluent can be prepared, and the electromagnetic valve 3 is started, and the antibody diluent can be discharged.
Although embodiments of the present utility model have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made therein without departing from the principles and spirit of the utility model, the scope of which is defined in the appended claims and their equivalents.
Claims (8)
1. The utility model provides a mixing stirring device of antibody diluent, includes blending tank (1), be provided with in blending tank (1) and be used for carrying out the stirring subassembly that stirs and mix to the stoste in blending tank (1), the both sides of blending tank (1) are provided with inlet pipe (2) and electromagnetic valve (3) respectively, a serial communication port, be provided with two first springs (4) and first damping jar (5) in blending tank (1), the other end of first spring (4) and first damping jar (5) is provided with fender piece (6), fender piece (6) offsets with the one end of inlet pipe (2).
2. The mixing and stirring device for antibody diluents according to claim 1, characterized in that a sliding piece (7) is arranged in the mixing tank (1), a buffer piece is arranged below the sliding piece (7), the buffer piece comprises two sliding blocks (8), a second spring (12) and a second damping cylinder (13), a sliding rail is arranged at the bottom of the sliding piece (7), the sliding blocks (8) are slidably connected in the sliding rail, two ends of the second spring (12) and the second damping cylinder (13) are respectively connected with the sliding blocks (8) and the sliding rail, and the sliding blocks (8) are connected with the inner bottom wall of the mixing tank (1).
3. The mixing and stirring device for antibody diluents according to claim 2, characterized in that a mounting seat (9) is arranged at the bottom of the sliding block (8), two fixing seats (10) are arranged in the mixing tank (1), a rotating piece (11) is rotationally arranged in the mounting seat (9), and the other end of the rotating piece (11) is rotationally arranged in the fixing seat (10).
4. A mixing and stirring device for antibody diluents according to claim 3, characterized in that the mounting base (9) and the fixing base (10) are respectively provided with a first pin and a second pin, and two ends of the rotating member (11) are respectively and rotatably sleeved on the first pin and the second pin.
5. The mixing and stirring device for antibody diluents according to claim 1, characterized in that the stirring assembly comprises a fixed plate (14), a motor (17), a transmission assembly and a plurality of stirring rods (16), wherein the fixed plate (14) is arranged in the mixing tank (1) and the bottom of the fixed plate is rotationally connected with two rotating shafts (15), the stirring rods (16) are arranged on the rotating shafts (15), and the motor (17) is arranged at the top of the fixed plate (14) and is in transmission connection with one of the rotating shafts (15), and the transmission assembly is connected with the rotating shafts (15).
6. The mixing and stirring device for antibody diluents according to claim 5, wherein the transmission assembly comprises a transmission chain (19) and two chain wheels (18), the chain wheels (18) are fixedly sleeved on the rotating shaft (15), and the transmission chain (19) is in meshed connection with the two chain wheels (18).
7. Mixing and stirring device for antibody diluents according to claim 1, characterized in that the bottom of the mixing tank (1) is provided with a plurality of underframes (20).
8. The mixing and stirring device for antibody diluents according to claim 2, wherein sealing strips are arranged on the periphery of the sliding piece (7), and the sealing strips are abutted against the inner wall of the mixing tank (1).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202321433479.9U CN220425231U (en) | 2023-06-07 | 2023-06-07 | Mixing stirring device for antibody diluent |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202321433479.9U CN220425231U (en) | 2023-06-07 | 2023-06-07 | Mixing stirring device for antibody diluent |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN220425231U true CN220425231U (en) | 2024-02-02 |
Family
ID=89700807
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202321433479.9U Active CN220425231U (en) | 2023-06-07 | 2023-06-07 | Mixing stirring device for antibody diluent |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN220425231U (en) |
-
2023
- 2023-06-07 CN CN202321433479.9U patent/CN220425231U/en active Active
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