CN220413422U - Full-automatic suspension cell culture bag suit - Google Patents
Full-automatic suspension cell culture bag suit Download PDFInfo
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- CN220413422U CN220413422U CN202321844622.3U CN202321844622U CN220413422U CN 220413422 U CN220413422 U CN 220413422U CN 202321844622 U CN202321844622 U CN 202321844622U CN 220413422 U CN220413422 U CN 220413422U
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- bag
- cell
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- 238000004113 cell culture Methods 0.000 title claims abstract description 60
- 239000000725 suspension Substances 0.000 title claims abstract description 24
- 239000007788 liquid Substances 0.000 claims abstract description 78
- 230000020411 cell activation Effects 0.000 claims abstract description 45
- 239000002699 waste material Substances 0.000 claims abstract description 23
- 239000001963 growth medium Substances 0.000 claims description 17
- 238000002347 injection Methods 0.000 claims description 15
- 239000007924 injection Substances 0.000 claims description 15
- 239000011248 coating agent Substances 0.000 claims description 11
- 238000000576 coating method Methods 0.000 claims description 11
- 239000012530 fluid Substances 0.000 claims description 8
- 239000000463 material Substances 0.000 claims description 7
- 238000001514 detection method Methods 0.000 claims description 6
- 238000005070 sampling Methods 0.000 claims description 5
- 238000002835 absorbance Methods 0.000 claims description 3
- 230000003213 activating effect Effects 0.000 claims description 3
- 229920000669 heparin Polymers 0.000 claims description 3
- ZFGMDIBRIDKWMY-PASTXAENSA-N heparin Chemical compound CC(O)=N[C@@H]1[C@@H](O)[C@H](O)[C@@H](COS(O)(=O)=O)O[C@@H]1O[C@@H]1[C@@H](C(O)=O)O[C@@H](O[C@H]2[C@@H]([C@@H](OS(O)(=O)=O)[C@@H](O[C@@H]3[C@@H](OC(O)[C@H](OS(O)(=O)=O)[C@H]3O)C(O)=O)O[C@@H]2O)CS(O)(=O)=O)[C@H](O)[C@H]1O ZFGMDIBRIDKWMY-PASTXAENSA-N 0.000 claims description 3
- 229960001008 heparin sodium Drugs 0.000 claims description 3
- 229920001296 polysiloxane Polymers 0.000 claims description 3
- 210000004027 cell Anatomy 0.000 description 52
- 238000000034 method Methods 0.000 description 7
- 230000008569 process Effects 0.000 description 4
- 230000009471 action Effects 0.000 description 3
- 239000002609 medium Substances 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 239000000243 solution Substances 0.000 description 2
- 241000894006 Bacteria Species 0.000 description 1
- 230000004075 alteration Effects 0.000 description 1
- 230000004663 cell proliferation Effects 0.000 description 1
- 238000012864 cross contamination Methods 0.000 description 1
- 238000012136 culture method Methods 0.000 description 1
- 238000012258 culturing Methods 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 230000002349 favourable effect Effects 0.000 description 1
- 208000015181 infectious disease Diseases 0.000 description 1
- 238000011081 inoculation Methods 0.000 description 1
- 210000000822 natural killer cell Anatomy 0.000 description 1
- 231100001083 no cytotoxicity Toxicity 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 230000001502 supplementing effect Effects 0.000 description 1
- 230000001988 toxicity Effects 0.000 description 1
- 231100000419 toxicity Toxicity 0.000 description 1
Landscapes
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The utility model provides a full-automatic suspension cell culture bag set, which comprises a primary cell storage bag, an initial cell activation bag, a waste liquid bag and a cell culture bag, wherein all bag bodies are connected through a three-way joint and a plurality of pipelines.
Description
Technical Field
The utility model relates to the field of cell culture, in particular to a culture bag set for culturing suspension cells, and particularly relates to a bag body structure with different purposes and a related connection mode.
Background
Conventional suspension cell (e.g., NK cells, 293, CHO, K562) culture is mainly performed using a cell culture flask and a cell culture bag, by adding initial cells/inoculations and corresponding media to the cell culture flask or cell culture bag, a cell culture environment is formed, and growth and propagation are maintained under appropriate circumstances. Currently, when the initial amount of cells is low, the culture is performed using a cell culture flask, and when the cell number is sufficient, the cells are transferred to a new culture bag for culture until the desired cell number is expanded. The culture method mainly uses manual operation, and the transfer operation of liquid and culture medium is required to be carried out for many times, so that the operation is complicated, and the risk of bacteria infection is easy to increase.
Disclosure of Invention
In view of the technical problems set forth in the background art, the present utility model provides a kit for fully automatic suspension cell activation culture, so as to solve at least one technical problem set forth in the background art.
In order to achieve the above purpose, the present utility model provides the following technical solutions:
the utility model relates to a full-automatic suspension cell activation culture set, wherein the cell culture bag set comprises:
a primary cell storage bag for holding primary cells, the primary cell storage bag comprising a primary cell storage bag body, an injection port, and a transfer port;
the initial cell activation bag is used for activating initial suspension cells and comprises an initial cell activation bag main body, a coating liquid injection port, a culture medium, a cell liquid inlet and a liquid transfer port;
the waste liquid bag is used for collecting waste liquid in the initial cell activation bag and comprises a waste liquid bag main body and a liquid inlet;
the cell culture bag is used for carrying out large-volume cell culture and comprises a cell culture bag main body, a liquid inlet and a sampling port;
the primary cell storage bag is connected with the culture medium and the cell liquid inlet of the primary cell activation bag through pipelines, and the liquid outlet of the primary cell activation bag is connected with the liquid inlet of the waste liquid bag and the liquid inlet of the cell culture bag through pipelines respectively.
As a further preferable embodiment, at least one of the primary cell storage bag injection port and the primary cell activation bag coating liquid injection port adopts a heparin sodium cap.
As a further preferable technical scheme, the cell culture bag further comprises a cell density detection port, wherein the cell density detection port is made of a light-transmitting material, and can detect the cell density through the absorbance of the liquid in the culture bag.
As a further preferable technical scheme, the cell activation bag further comprises four-way connectors, 3 connectors in the four-way connectors are respectively connected with 3 culture medium liquid inlet pipelines, and a 4 th connector of the four-way connectors is connected with a culture medium and a cell liquid inlet of the initial cell activation bag through pipelines.
As a further preferable technical scheme, the culture medium cell activation bag further comprises a three-way joint A, a pipeline used for connecting the rotary outlet of the primary cell storage bag, a pipeline connected with the 4 th joint of the four-way joint, and a culture medium and cell liquid inlet pipeline of the primary cell activation bag.
As a further preferable technical scheme, the device further comprises a three-way joint B for connecting the liquid transfer outlet pipeline of the initial cell activation bag, the liquid inlet pipeline of the waste liquid bag and the liquid inlet pipeline of the cell culture bag.
As a further preferable technical scheme, at least one of the primary cell storage bag transfer outlet, the culture medium and cell liquid inlet and liquid transfer outlet of the initial cell activation bag, and the liquid inlet of the cell culture bag is connected with a pipeline by a luer connector.
As a further preferable technical scheme, the pipeline adopts a silicone tube.
As a further preferable technical scheme, at least one bag body of the primary cell storage bag, the primary cell activation bag, the waste liquid bag and the cell culture bag is made of FEP waterproof breathable film materials.
As a further preferable embodiment, the volume capacity of the cell culture bag is 2L or more.
The utility model solves the defect that the existing suspension cell culture needs to manually transfer liquid for multiple times, provides a full-automatic suspension cell activation culture suit system, ensures the sterile environment of cell culture, can be matched with equipment to observe the cell density under the condition of not sampling, and overcomes the risk of cross contamination of cells in manual operation engineering.
Drawings
FIG. 1 is a schematic diagram of a fully automated suspension cell culture bag kit according to an embodiment of the utility model.
In the figure: 1-primary cell storage bag, 101-primary cell storage bag main body, 102-injection port, 103-transfer port, 2-primary cell activation bag, 201-primary cell activation bag main body, 202-coating liquid injection port, 203-culture medium and cell liquid inlet port, 204-liquid transfer port, 3-waste liquid bag, 301-waste liquid bag main body, 302-liquid inlet port, 4-cell culture bag, 401-cell culture bag main body, 402-liquid inlet port, 403-sampling port, 404-cell density detection port, 501-507-pipeline, 601-three-way joint A, 602-three-way joint B, 701-four-way joint.
Detailed Description
Features and exemplary embodiments of various aspects of the present utility model are described in detail below, and in order to make the objectives, technical solutions, and advantages of the present utility model more apparent, the present utility model is described in further detail below with reference to the accompanying drawings and examples. It should be understood that the specific embodiments described herein are merely configured to explain the present patent and are not configured to limit the present patent. It will be apparent to one skilled in the art that the present utility model may be practiced without some of these specific details. The following description of the embodiments is merely intended to provide a better understanding of the present utility model by showing examples of the present utility model.
It is noted that relational terms such as first and second, and the like are used solely to distinguish one entity or action from another entity or action without necessarily requiring or implying any actual such relationship or order between such entities or actions. Moreover, the terms "comprises," "comprising," or any other variation thereof, are intended to cover a non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements does not include only those elements but may include other elements not expressly listed or inherent to such process, method, article, or apparatus. Without further limitation, an element defined by the phrase "comprising … …" does not exclude the presence of other like elements in a process, method, article or apparatus that comprises the element.
Referring to FIG. 1, a full-automatic suspension cell culture bag set according to the present utility model comprises a primary cell storage bag 1, a primary cell activation bag 2, a waste liquid bag 3 and a cell culture bag 4. Preferably, the primary cell storage bag 1, the primary cell activation bag 2, the waste liquid bag 3 and the cell culture bag 4 are made of FEP waterproof and breathable film materials, the FEP waterproof and breathable film has stable chemical properties, no cytotoxicity and tissue toxicity, is favorable for cell proliferation, is transparent, and is easy to microscopic observation.
Wherein the primary cell storage bag 1 is used for storing primary cells, and the volume capacity is preferably 20ml, the primary cell storage bag 1 comprises a primary cell storage bag main body 101, an injection port 102 and a transfer outlet 103. The primary cell activation bag 2 is used for activating primary suspension cells, and comprises a primary cell activation bag main body 201, a coating liquid injection port 202, a culture medium and cell liquid inlet 203 and a liquid transfer port 204. The above-described waste liquid bag 3 is for collecting waste liquid in an initial cell activation bag, and includes a waste liquid bag main body 301 and a liquid inlet 302. The cell culture bag 4 is used for large-volume cell culture, and preferably has a volume capacity of 2L or more, and includes a cell culture bag body 401, a liquid inlet 402, and a sampling port 403.
The primary cell storage bag 1, the primary cell activation bag 2, the waste liquid bag 3 and the cell culture bag 4 are connected through a three-way joint A601, a three-way joint B602 and a plurality of pipelines 501-507, and the pipelines 501-507 are preferably made of silicone tubes.
Specifically, the outlet 103 of the primary cell storage bag 1 is connected to the medium and cell feed port 203 of the primary cell activation bag 2 through the lines 501, 502, and the liquid outlet 204 of the primary cell activation bag 2 is connected to the feed port 302 of the waste liquid bag 3 and the cell culture bag feed port 402 through the lines 503, 504, 505, respectively.
Wherein lines 501 and 502 are connected by tee joint a 601 and lines 503, 504, 505 are connected by tee joint B602.
Further, the primary cell storage bag 1 injection port 102 and the primary cell activation bag 2 coating liquid injection port 202 are heparin sodium caps. The injection port is a channel for manually adding primary cells into the bag body, so that the contact between the interior of the bag body and the external environment is reduced, and the pollution risk is reduced. The filling opening is a channel for manually adding coating liquid, so that the liquid can be directly added into the bag body, the loss of the coating liquid in a pipeline is avoided, more effective coating of the bag body material is facilitated, and the initial cell activation effect is improved.
Further, the cell culture bag 4 further comprises a cell density detection port 404, and the cell density detection port 404 is made of a light-transmitting material, so that the cell density can be detected by the absorbance of the liquid in the culture bag.
Further, the cell culture bag set further comprises a four-way connector 701, wherein 3 connectors in the four-way connector 701 are respectively connected with 3 culture medium liquid inlet pipelines 506, and a 4 th connector of the four-way connector is connected with the culture medium and the cell liquid inlet 203 of the initial cell activation bag 2 through pipelines 507 and 502 and a three-way connector A601.
Further, the three-way joint A601 connects the line 501 connected to the rotation outlet 103 of the primary cell storage bag 1, the line 507 connected to the 4 th joint of the four-way joint 701, and the medium and cell inlet line 502 of the primary cell activation bag 2.
The three-way joint B602 connects the liquid transfer outlet line 503 of the initial cell activation bag 2, the waste liquid bag inlet line 504, and the cell culture bag inlet line 505.
Further, the primary cell storage bag transfer port 103, the culture medium inlet 203 and the liquid transfer port 204 of the primary cell activation bag, and the liquid inlet 402 of the cell culture bag are connected to the pipeline by luer connectors.
In the using process of the cell culture bag set product provided by the utility model, only the used culture medium is connected with the liquid inlet, primary cells are injected into the cell culture bag, and the antibody coating liquid is injected into the initial cell activation bag, so that the preparation work in the earlier stage is realized. The whole set of bag body is placed in an automatic culture workstation, and the transfer of cells in the bag body and the culture medium fluid supplementing operation are carried out through the interconnection between the pipelines and the bag body. Greatly reduces the steps of manual operation and improves the whole cell culture efficiency.
It will be apparent to those skilled in the art that various modifications and variations can be made to the embodiments of the present utility model without departing from the spirit and scope of the utility model. Thus, it is intended that the present utility model also include such modifications and alterations insofar as they come within the scope of the appended claims or the equivalents thereof.
Claims (10)
1. A fully automated suspension cell culture bag set, comprising:
a primary cell storage bag for holding primary cells, the primary cell storage bag comprising a primary cell storage bag body, an injection port, and a transfer port;
the initial cell activation bag is used for activating initial suspension cells and comprises an initial cell activation bag main body, a coating liquid injection port, a culture medium, a cell liquid inlet and a liquid transfer port;
the waste liquid bag is used for collecting waste liquid in the initial cell activation bag and comprises a waste liquid bag main body and a liquid inlet;
the cell culture bag is used for carrying out large-volume cell culture and comprises a cell culture bag main body, a liquid inlet and a sampling port;
the primary cell storage bag is connected with the culture medium and the cell liquid inlet of the primary cell activation bag through pipelines, and the liquid outlet of the primary cell activation bag is connected with the liquid inlet of the waste liquid bag and the liquid inlet of the cell culture bag through pipelines respectively.
2. The fully automated suspension cell culture bag set of claim 1 wherein at least one of the primary cell storage bag injection port and the primary cell activation bag coating fluid injection port employs a heparin sodium cap.
3. The fully automated suspension cell culture bag set of claim 2 wherein the cell culture bag further comprises a cell density detection port made of a light transmissive material, the cell density being detectable by absorbance of a liquid in the culture bag.
4. The fully automatic suspension cell culture bag set of claim 1 further comprising four-way connectors, wherein 3 connectors in the four-way connectors are respectively connected with 3 media feed liquid pipelines, and the 4 th connector of the four-way connectors is connected with the media and the cell feed liquid port of the initial cell activation bag through pipelines.
5. The fully automated suspension cell culture bag set of claim 4 further comprising a three-way connector a for connecting the tubing connected to the swivel outlet of the primary cell storage bag, the tubing connected to the 4 th connector of the four-way connector, and the media and cell feed inlet tubing of the primary cell activation bag.
6. The fully automated suspension cell culture bag set of claim 1 further comprising a three-way connector B for connecting the fluid transfer outlet line of the initial cell activation bag, the waste bag fluid inlet line, and the cell culture bag fluid inlet line.
7. The fully automated suspension cell culture bag set of claim 6 wherein at least one of the primary cell storage bag transfer port, the media and cell fluid inlet and fluid transfer port of the primary cell activation bag, and the fluid inlet of the cell culture bag is connected to tubing using a luer fitting.
8. The fully automated suspension cell culture bag set of any one of claims 1-7 wherein the tubing is silicone tubing.
9. The fully automated suspension cell culture bag set of any one of claims 1-7 wherein at least one of the primary cell storage bag, primary cell activation bag, waste bag and cell culture bag is made of FEP waterproof breathable film material.
10. The fully automated suspension cell culture bag set of any one of claims 1-7 wherein the cell culture bag has a volumetric capacity of 2L and greater.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202321844622.3U CN220413422U (en) | 2023-07-14 | 2023-07-14 | Full-automatic suspension cell culture bag suit |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202321844622.3U CN220413422U (en) | 2023-07-14 | 2023-07-14 | Full-automatic suspension cell culture bag suit |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN220413422U true CN220413422U (en) | 2024-01-30 |
Family
ID=89657558
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202321844622.3U Active CN220413422U (en) | 2023-07-14 | 2023-07-14 | Full-automatic suspension cell culture bag suit |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN220413422U (en) |
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2023
- 2023-07-14 CN CN202321844622.3U patent/CN220413422U/en active Active
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