CN220357316U

CN220357316U - Normal and inverted observation microscope

Info

Publication number: CN220357316U
Application number: CN202322037175.7U
Authority: CN
Inventors: 朱爱巧; 张任之
Original assignee: Shengsitai Instrument Suzhou Co ltd
Current assignee: Shengsitai Instrument Suzhou Co ltd
Priority date: 2023-07-31
Filing date: 2023-07-31
Publication date: 2024-01-16
Anticipated expiration: 2033-07-31

Abstract

The utility model belongs to the technical field of biological instruments, and particularly relates to a normal and inverted observation microscope, wherein a supporting plate is vertically arranged on a base, and a lens frame can be hinged at the upper end of the supporting plate in a left-right rotating manner; the objective table can be arranged on the lens holder in a vertical sliding manner, is perpendicular to the optical axis of the objective lens acquisition module, and can realize direct conversion between the inverted microscope and the inverted microscope by turning the lens holder left and right, so that the use is convenient. The lifting of the objective table is regulated by the lifting regulating mechanism, so that quick focusing can be performed, and the imaging requirements of an inverted microscope and the inverted microscope are met. The image acquisition module directly acquires the image information of the objective acquisition module, removes the eyepiece structure of the conventional microscope, directly images the camera in real time through a display screen or a computer, has a simpler structure, and is easy to switch the positive and negative microscope.

Description

Normal and inverted observation microscope

Technical Field

The utility model belongs to the technical field of biological instruments, and particularly relates to a normal and inverted observation microscope.

Background

Upright biomicroscopes are the most common biomicroscopes in laboratories and classrooms, with the objective lens changer facing downward and the stage below the objective lens. When observing an object, the object to be observed is placed on the object stage, the objective lens is close to the glass slide from above to observe, the working distance is relatively short, and the observation section is suitable for a forward biological microscope.

The objective of the inverted microscope is up and the stage is above the objective. This microscope is most suitable when observing living cells, because the working distance of the forward biological microscope is short, and there is no way to observe living cells in the culture dish. The culture dish can be observed by the inverted microscope only by placing the culture dish on the object stage, and the living cells in the culture dish can be easily observed due to the fact that the light path of the inverted microscope is reversed and the working distance of the condenser is long.

In order to realize that a microscope has two functions of upright and inverted, chinese patent document CN201621461708.8 (201621461708.8) discloses a biological microscope, which includes: the device comprises a base, an ocular and an objective lens; the ocular lens is arranged on the base through an ocular lens cone, and a hollow rotating shaft is arranged in the middle of the ocular lens cone; an L-shaped objective lens barrel is arranged on the rotating shaft; an objective lens is arranged at one end of the objective lens barrel, which is far away from the rotating shaft, and the objective lens faces the extension line of the rotating shaft; the object lens cone can rotate by taking the rotating shaft as an axis, and the object lens cone are internally provided with a reflection lens group for reflecting the field of view of the objective lens to the ocular lens; the base is also provided with a bracket, and the bracket is provided with an objective table; an illuminating lamp is arranged beside the objective lens. The above patent realizes the normal observation and the inverted observation by rotating the objective lens, and even can realize the oblique observation, thereby being suitable for different culture vessels. However, when the angle of the objective lens is changed, the objective table and the LED illumination plate are required to be detached from the bracket for reordering, so that the structure is inconvenient to use. In addition, the L-shaped objective lens barrel increases the light transmission distance between the object and the ocular lens, and causes loss of imaging quality of the objective lens. And when the objective lens rotates below the object, the transmission illumination white light illumination unit needs to be disassembled, and the illumination angle of the white light illumination unit cannot be adjusted rotationally around the object, so that the use is further inconvenient.

Disclosure of Invention

The utility model aims to provide a normal and inverted observation microscope, wherein an objective lens acquisition module, a light source module, an objective table, a graph acquisition module and a lifting adjusting mechanism are arranged on a lens holder, the lens holder can be hinged to the upper end of a supporting plate in a left-right rotating manner, and the lens holder can be turned left and right to realize the rapid conversion of the inverted microscope and an inverted microscope.

In order to achieve the above purpose, the present utility model provides the following technical solutions: a normal and inverted observation microscope comprises a base, a supporting plate, a lens holder, an objective lens acquisition module, a light source module, an objective table, a graph acquisition module and a lifting adjusting mechanism, wherein the objective lens acquisition module, the light source module, the objective table, the graph acquisition module and the lifting adjusting mechanism are arranged on the lens holder;

the supporting plate is vertically arranged on the base, and the spectacle frame can be hinged to the upper end of the supporting plate in a left-right rotating manner;

the objective table is arranged on the lens holder in a vertically sliding manner, is perpendicular to the optical axis of the objective lens acquisition module, and is used for adjusting the lifting of the objective table;

the light source module is used for providing illumination light for optical imaging of the objective lens acquisition module;

the image acquisition module is arranged on the lens frame and is communicated with the optical path of the objective acquisition module, and the image acquisition module is used for acquiring the image information of the objective acquisition module.

The technical scheme of the utility model is as follows: the light source module comprises a white light illumination unit;

the transmitted illumination light of the white light illumination unit irradiates an object on the object stage and then is transmitted to the object lens acquisition module. The white light illumination unit provides illumination for a common optical microscope that observes an object with a lens combination magnified several hundred to 1000 times.

The technical scheme of the utility model is as follows: the light source module comprises an excitation block module and a fluorescent lighting unit;

the excitation block module is positioned between the pattern acquisition module and the objective acquisition module, and the fluorescent lighting unit is connected with the excitation block module;

the fluorescent illumination light of the fluorescent illumination unit sequentially passes through the excitation block module and the objective lens acquisition module and irradiates on an object on the object table, and the light emitted by the object on the object table is sequentially transmitted to the pattern acquisition module through the objective lens acquisition module and the excitation block module. The fluorescence microscope uses ultraviolet rays as a light source to irradiate the object to be inspected so as to emit fluorescence, and then the shape and the position of the object are observed under the microscope. Fluorescence microscopy is used to study the absorption, transport, distribution of chemical substances, localization of intracellular substances, etc. Some substances in the cells, such as chlorophyll and the like, can fluoresce when irradiated by ultraviolet rays; while some other substances cannot fluoresce by themselves, fluorescent microscopy is one of the tools for qualitative and quantitative studies of such substances, if they are stained with fluorescent dyes or fluorescent antibodies, they fluoresce upon irradiation with ultraviolet light. In order to realize the function of a fluorescence microscope, an excitation block module and a fluorescence illumination unit are arranged on an objective lens acquisition module, and fluorescence illumination light emitted by the fluorescence illumination unit is reflected into the objective lens acquisition module at right angles after passing through the excitation block module and then reaches an object on an objective table; the object can emit light in addition, passes through the excitation block module after being collected by the objective lens collection module, is collected and received by the graph collection module, and then is transmitted to the PC end to display images.

The technical scheme of the utility model is as follows: the supporting plate can be horizontally and rotatably arranged on the base. Through the horizontal rotation backup pad on the base, can adjust the angle of use of microscope at any time to satisfy double or many people and visit altogether. Double or multiple people in the same sample under the same visual field can observe in the right or inverted mode (such as a microscope is arranged on the two sides of a table top and double sitting), and compared with the conventional double co-viewing, the method is more suitable for direct adjustment of the microscope (such as a focusing wheel and a visual field area) by an observer.

The technical scheme of the utility model is as follows: the lifting adjusting mechanism comprises a coarse focusing spiral and a fine focusing spiral which are rotatably arranged on the lens frame;

the objective table is in sliding connection with a sliding rail vertically arranged on the mirror bracket, the coarse focusing screw and the fine focusing screw are respectively in linkage with the objective table, and the objective table is enabled to slide up and down along the sliding rail by rotating the coarse focusing screw or the fine focusing screw. The coarse focusing spiral and the fine focusing spiral are respectively rotated to quickly or finely adjust the upper and lower positions of the objective table, so that the focusing device is suitable for different focusing speeds.

The technical scheme of the utility model is as follows: the object stage comprises an XY-axis two-dimensional moving platform. The XY-axis two-dimensional moving platform is arranged, so that the position of an object on the object stage is adjusted along the X axis or the Y axis in the horizontal direction, and the object is positioned in the observation visual field range.

The technical scheme of the utility model is as follows: the XY-axis two-dimensional moving platform comprises a bearing plate, an X-direction moving plate and a Y-direction moving plate, wherein the bearing plate, the X-direction moving plate and the Y-direction moving plate all adopt thin plate structures;

the X-direction moving plate is arranged on the bearing plate in a back-and-forth sliding way, and the Y-direction moving plate is arranged on the X-direction moving plate in a left-and-right sliding way;

the bearing plate is provided with a first through hole, the X-direction moving plate is provided with a second through hole, the Y-direction moving plate is provided with a third through hole, the second through hole and the third through hole are matched with the first through hole, and the second through hole, the third through hole and the first through hole all adopt a macroporous structure. Because the bearing plate, the X-direction moving plate and the Y-direction moving plate all adopt thin plate structures, the thickness of the objective table can be reduced, and the objective table is prevented from colliding with the periphery of other parts in the focal length adjusting process. The second through hole and the third through hole are matched with the first through hole, the second through hole, the third through hole and the first through hole are all of macroporous structures, and the second through hole, the third through hole and the first through hole are used as light holes, and objects are placed above the second through hole or above the third through hole respectively in the process of upright and inverted observation so as to meet the adjustment requirement of the visual field range.

The technical scheme of the utility model is as follows: the XY axis two-dimensional moving platform further comprises an XY object stage adjusting handle which is parallel to the bearing plate. The XY stage adjusting handle of the XY axis two-dimensional moving platform is designed by adopting a horizontal knob, and the relative position of the XY stage adjusting handle and a user is kept unchanged in the processes of overturning the spectacle frame left and right and horizontally rotating the supporting plate, so that the XY stage adjusting handle is more suitable for personnel to operate; conventional vertical handles can be inconvenient to use.

The technical scheme of the utility model is as follows: the graph acquisition module comprises a camera which is in communication connection with the computer end.

Compared with the prior art, the utility model has the beneficial effects that: the utility model vertically arranges the supporting plate on the base and hinges the spectacle frame on the upper end of the supporting plate in a way of left and right rotation; the objective table can be arranged on the lens holder in a vertical sliding manner, is perpendicular to the optical axis of the objective lens acquisition module, and can realize direct conversion between the inverted microscope and the inverted microscope by turning the lens holder left and right, so that the use is convenient.

The lifting of the objective table is regulated by the lifting regulating mechanism, so that quick focusing can be performed, and the imaging requirements of an inverted microscope and the inverted microscope are met.

The image acquisition module directly acquires the image information of the objective acquisition module, removes the eyepiece structure of the conventional microscope, directly images the camera in real time through a display screen or a computer, has a simpler structure, and is easy to switch the positive and negative microscope.

Drawings

FIG. 1 is a schematic view of the structure of an inverted viewing microscope according to the present utility model;

FIG. 2 is a perspective view of an inverted viewing microscope according to the present utility model;

FIG. 3 is a schematic view of a stage according to the present utility model;

in the figure: 1 base, 2 support plate, 3 lens holder, 4 objective lens acquisition module, 5 objective table, 6 graph acquisition module;

the device comprises a white light lighting unit 7, an excitation block module 8, a fluorescent lighting unit 9, a coarse focusing spiral 10 and a fine focusing spiral 11;

a 51 bearing plate, a 52X-direction moving plate, a 53Y-direction moving plate and a 54XY stage adjusting handle;

511 first via, 521 second via, 531 third via.

Detailed Description

The following describes specific embodiments of the present utility model in detail with reference to the drawings.

Example 1

As shown in fig. 1, a normal and inverted observation microscope comprises a base 1, a support plate 2, a lens holder 3, an objective lens acquisition module 4, a light source module, an objective table 5, a graph acquisition module 6 and a lifting adjusting mechanism, wherein the objective lens acquisition module 4, the light source module, the objective table 5, the graph acquisition module 6 and the lifting adjusting mechanism are arranged on the lens holder 3.

The support plate 2 is vertically arranged on the base 1, and the support plate 2 can be horizontally arranged on the base 1 in a 360-degree rotation mode. The spectacle frame 3 can be hinged at the upper end of the supporting plate 2 in a left-right rotating way.

The objective table 5 is arranged on the lens holder 3 in a vertically sliding manner, the objective table 5 is perpendicular to the optical axis of the objective lens acquisition module 4, and the lifting adjusting mechanism is used for adjusting the lifting of the objective table 5.

The light source module is used for providing illumination light for optical imaging of the objective lens acquisition module 4. The image acquisition module 6 is arranged on the lens frame 3 and is communicated with the optical path of the objective acquisition module 4, and the image acquisition module 6 is used for acquiring the image information of the objective acquisition module 4.

The light source module comprises a white light illumination unit 7. Specifically, the mirror holder 3 includes U type shell, and backup pad 2 upper end detachable is provided with the pivot, U type shell intermediate position rotatable setting is in the pivot, objective collection module 4 and white light lighting element 7 set up respectively at the both ends of U type shell, objective table 5 sets up between objective collection module 4 and white light lighting element 7.

The transmitted illumination light of the white light illumination unit 7 irradiates the object on the object stage 5 and then is transmitted to the objective lens acquisition module 4. The objective lens collection module 4 includes an objective lens changer and an objective lens provided on the objective lens changer, and selects objective lenses of different magnification by rotating the objective lens changer. The white light illumination unit 7 includes a white light source and a condenser lens, which can be correspondingly matched according to the magnification of the lens of the objective lens, i.e., a condenser lens converter and a condenser lens disposed on the condenser lens converter are disposed in the condenser lens, and a suitable condenser lens is selected by rotating the condenser lens converter.

The image acquisition module 6 is connected with a PC end, namely a computer, the PC end is used for displaying and storing images acquired by the image acquisition module 6, and the PC end can also control an electric module of the inverted observation microscope according to the needs. The transmitted illumination light emitted by the white light illumination unit 7 passes through the object on the object stage 5 after being focused by the condenser, then passes through the objective lens of the objective lens acquisition module 4, is acquired and received by the graph acquisition module 6, and then is transmitted to the PC end to display an image. The graphic acquisition module 6 specifically comprises a camera which is in communication connection with a computer end. The camera is arranged inside the U-shaped shell.

As shown in fig. 2, the elevation adjustment mechanism includes a coarse focusing screw 10 and a fine focusing screw 11 rotatably provided on the frame 3.

The objective table 5 is in sliding connection with a sliding rail vertically arranged on the mirror bracket 3, the coarse focusing spiral 10 and the fine focusing spiral 11 are respectively arranged in linkage with the objective table 5, and the objective table 5 vertically slides along the sliding rail by rotating the coarse focusing spiral 10 or the fine focusing spiral 11. The lifting speed of the coarse focusing spiral 10 on the object stage 5 is greater than the lifting speed of the fine focusing spiral 11 on the object stage 5.

The stage 5 includes an XY-axis two-dimensional moving stage.

Specifically, as shown in fig. 3, the XY-axis two-dimensional moving platform includes a carrying plate 51, an X-direction moving plate 52, and a Y-direction moving plate 53.

The X-direction moving plate 52 is slidably disposed on the carrier plate 51, the Y-direction moving plate 53 is slidably disposed on the X-direction moving plate 52, and both the X-direction moving plate 52 and the Y-direction moving plate 53 are located in the edge of the carrier plate 51 within the range of the adjustment travel. Because the X-direction moving plate 52 and the Y-direction moving plate 53 are both positioned in the edge of the bearing plate 51 in the range of the adjusting stroke, the excessive space occupied by the object stage 5 in the process of adjusting the field of view can be effectively avoided, and the microscope structure is more compact.

The bearing plate 51 is provided with a first through hole 511, the X-direction moving plate 52 is provided with a second through hole 521, the Y-direction moving plate 53 is provided with a third through hole 531, and the second through hole 521 and the third through hole 531 are matched with the first through hole 511. The carrying plate 51, the X-direction moving plate 52 and the Y-direction moving plate 53 all adopt thin plate structures, the second through hole 521, the third through hole 531 and the first through hole 511 all adopt macroporous structures, specifically rectangular holes, and the second through hole 521 and the third through hole 531 occupy more than 70% of the area of the respective plates. The objective table 5 is designed to be thin and large in hole, after the eyeglass frame 3 is overturned left and right, an observation sample is placed on the objective table 5, so that the XY displacement range (the observed sample visual field range) of the sample can be ensured to meet the observation requirement, and the objective table 5 is prevented from colliding with the periphery of other parts in the focusing process.

The XY-axis two-dimensional moving platform further includes an XY stage adjustment handle 54 parallel to the carrier plate 51.

Example 2

As shown in fig. 1, the light source module is different from embodiment 1 in that it includes an excitation block module 8 and a fluorescent lighting unit 9. The excitation block module 8 and the fluorescent lighting unit 9 are both arranged inside the U-shaped housing.

The excitation block module 8 is located between the pattern acquisition module 6 and the objective acquisition module 4, and the fluorescent lighting unit 9 is connected with the excitation block module 8. The excitation block module 8 comprises an excitation block converter and excitation blocks arranged on the excitation block converter, wherein the excitation block converter is provided with gaps, namely, in order to ensure that the transmission illumination light emitted by the white light illumination unit 7 passes through, different excitation blocks can be selected by rotating the excitation block converter, and the gaps can be switched to realize the illumination of the transmission illumination light.

The fluorescence illumination light of the fluorescence illumination unit 9 sequentially irradiates on the object table 5 through the excitation block module 8 and the object lens acquisition module 4, and the light emitted by the object on the object table 5 sequentially passes through the object lens acquisition module 4 and the excitation block module 8 and is transmitted to the pattern acquisition module 6. The fluorescent illumination light emitted by the fluorescent illumination unit 9 is reflected by the excitation block of the excitation block module 8 at right angles to enter the objective lens of the objective acquisition module 4 and then reaches the object on the objective table 5; the object emits light, passes through the excitation block of the excitation block module 8 after being collected by the objective lens of the objective lens collecting module 4, is collected and received by the pattern collecting module 6, and is then transmitted to the PC end to display an image.

Claims

1. A positive inversion viewing microscope, characterized by: the device comprises a base (1), a supporting plate (2), a lens holder (3), an objective lens acquisition module (4) arranged on the lens holder (3), a light source module, an objective table (5), a graph acquisition module (6) and a lifting adjusting mechanism;

the support plate (2) is vertically arranged on the base (1), and the spectacle frame (3) can be hinged at the upper end of the support plate (2) in a left-right rotating way;

the objective table (5) is arranged on the lens holder (3) in a vertically sliding manner, the objective table (5) is perpendicular to the optical axis of the objective lens acquisition module (4), and the lifting adjusting mechanism is used for adjusting the lifting of the objective table (5);

the light source module is used for providing illumination light for optical imaging of the objective lens acquisition module (4);

the image acquisition module (6) is arranged on the lens frame (3) and is communicated with the optical path of the objective acquisition module (4), and the image acquisition module (6) is used for acquiring the image information of the objective acquisition module (4).

2. The positive inverted viewing microscope of claim 1, wherein: the light source module comprises a white light illumination unit (7);

the transmitted illumination light of the white light illumination unit (7) irradiates an object on the object stage (5) and then is transmitted to the object lens acquisition module (4).

3. The positive inverted viewing microscope of claim 1, wherein: the light source module comprises an excitation block module (8) and a fluorescent lighting unit (9);

the excitation block module (8) is positioned between the pattern acquisition module (6) and the objective acquisition module (4), and the fluorescent lighting unit (9) is connected with the excitation block module (8);

the fluorescent illumination light of the fluorescent illumination unit (9) sequentially passes through the excitation block module (8) and the objective lens acquisition module (4) to irradiate on an object on the object table (5), and the light emitted by the object on the object table (5) sequentially passes through the objective lens acquisition module (4) and the excitation block module (8) to be transmitted to the pattern acquisition module (6).

4. The positive inverted viewing microscope of claim 1, wherein: the supporting plate (2) can be horizontally and rotatably arranged on the base (1).

5. The positive inverted viewing microscope of claim 1, wherein: the lifting adjusting mechanism comprises a coarse focusing spiral (10) and a fine focusing spiral (11) which are rotatably arranged on the lens frame (3);

the objective table (5) is in sliding connection with a sliding rail vertically arranged on the mirror bracket (3), the coarse focusing spiral (10) and the fine focusing spiral (11) are respectively arranged in linkage with the objective table (5), and the objective table (5) vertically slides along the sliding rail by rotating the coarse focusing spiral (10) or the fine focusing spiral (11).

6. The positive inverted viewing microscope of claim 1, wherein: the object stage (5) comprises an XY-axis two-dimensional moving platform.

7. The positive inverted viewing microscope of claim 6, wherein: the XY-axis two-dimensional moving platform comprises a bearing plate (51), an X-direction moving plate (52) and a Y-direction moving plate (53), wherein the bearing plate (51), the X-direction moving plate (52) and the Y-direction moving plate (53) are all of thin plate structures;

the X-direction moving plate (52) is arranged on the bearing plate (51) in a back-and-forth sliding way, and the Y-direction moving plate (53) is arranged on the X-direction moving plate (52) in a left-and-right sliding way;

the bearing plate (51) is provided with a first through hole (511), the X-direction moving plate (52) is provided with a second through hole (521), the Y-direction moving plate (53) is provided with a third through hole (531), the second through hole (521) and the third through hole (531) are matched with the first through hole (511), and the second through hole (521), the third through hole (531) and the first through hole (511) all adopt macroporous structures.

8. The positive inverted viewing microscope of claim 7, wherein: the XY-axis two-dimensional moving platform further comprises an XY stage adjusting handle (54) which is parallel to the bearing plate (51).

9. The positive inverted viewing microscope of claim 1, wherein: the graph acquisition module (6) comprises a camera which is in communication connection with a computer end.