CN220246140U - Induced pluripotent stem cell culture device - Google Patents
Induced pluripotent stem cell culture device Download PDFInfo
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- CN220246140U CN220246140U CN202321745014.7U CN202321745014U CN220246140U CN 220246140 U CN220246140 U CN 220246140U CN 202321745014 U CN202321745014 U CN 202321745014U CN 220246140 U CN220246140 U CN 220246140U
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- 210000004263 induced pluripotent stem cell Anatomy 0.000 title claims abstract description 19
- 238000004113 cell culture Methods 0.000 title claims abstract description 18
- 239000001963 growth medium Substances 0.000 claims abstract description 28
- 210000004027 cell Anatomy 0.000 claims abstract description 17
- 230000005540 biological transmission Effects 0.000 claims description 7
- 238000013016 damping Methods 0.000 claims description 4
- 230000003044 adaptive effect Effects 0.000 claims description 2
- 239000012530 fluid Substances 0.000 claims description 2
- 238000007789 sealing Methods 0.000 claims description 2
- 230000008878 coupling Effects 0.000 claims 1
- 238000010168 coupling process Methods 0.000 claims 1
- 238000005859 coupling reaction Methods 0.000 claims 1
- 238000010008 shearing Methods 0.000 abstract description 3
- 239000002609 medium Substances 0.000 description 4
- 230000005484 gravity Effects 0.000 description 3
- 230000003068 static effect Effects 0.000 description 3
- 230000008859 change Effects 0.000 description 2
- 239000004205 dimethyl polysiloxane Substances 0.000 description 2
- 230000007774 longterm Effects 0.000 description 2
- 238000000034 method Methods 0.000 description 2
- 229920000435 poly(dimethylsiloxane) Polymers 0.000 description 2
- 230000008569 process Effects 0.000 description 2
- 210000000130 stem cell Anatomy 0.000 description 2
- 239000002699 waste material Substances 0.000 description 2
- 108091003079 Bovine Serum Albumin Proteins 0.000 description 1
- 230000001464 adherent effect Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000015556 catabolic process Effects 0.000 description 1
- 230000010261 cell growth Effects 0.000 description 1
- 230000036755 cellular response Effects 0.000 description 1
- 238000011109 contamination Methods 0.000 description 1
- 239000012531 culture fluid Substances 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000006731 degradation reaction Methods 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 239000012091 fetal bovine serum Substances 0.000 description 1
- 238000001727 in vivo Methods 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 230000000813 microbial effect Effects 0.000 description 1
- 230000003278 mimic effect Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 210000000056 organ Anatomy 0.000 description 1
- 230000002093 peripheral effect Effects 0.000 description 1
- -1 polydimethylsiloxane Polymers 0.000 description 1
- 229960005322 streptomycin Drugs 0.000 description 1
- 239000000758 substrate Substances 0.000 description 1
- 239000010891 toxic waste Substances 0.000 description 1
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- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The utility model belongs to the technical field of cell culture, and discloses an induced pluripotent stem cell culture device, which comprises a microfluidic culture device arranged on a workbench, wherein the microfluidic culture device comprises a main channel communicated with a culture medium output tank and a culture medium receiving tank, a plurality of culture chambers are arranged on the main channel, each culture chamber is a cylindrical chamber, and a cell inlet and a cell outlet are respectively arranged on two sides of each culture chamber; the workbench comprises a table top and a driving box body which is hinged with the table top, one end of the table top is hinged with the driving box body through a fixed shaft, the other end of the table top is hinged with the driving box body through a sleeving device, the middle deflection sleeving device end of the table top is hinged with a driving rod of a driving device arranged in the driving box body through a supporting rod, and the top of the driving box body is provided with a hollowed-out groove which is matched with a driving rod running track; the bottom of the table top is also provided with an inclination sensor. The utility model does not generate high shearing force, continuously provides fresh culture medium, and solves the culture problem in the prior art.
Description
Technical Field
The utility model belongs to the technical field of cell culture, and particularly relates to an induced pluripotent stem cell culture device.
Background
Induced pluripotent stem cell cultures are typically performed under static conditions. Cells grow as adherent colonies on various planar substrates and periodically replace the medium, however, conventional static culture may not be able to determine the actual cellular response because it is unable to control and mimic the microenvironment of complex organs. The main drawbacks of static culture are the instability of the cell environment, due to the consumption of growth medium by the cells and the exudation of waste in the culture chamber, leading to toxic waste, dead cells, nutrient depletion and damage to the environment. Although daily medium changes refresh the environment degradation, this is also a sudden and dramatic change, which can put stress on the cells.
Microfluidic culture is now commonly used instead of traditional cell culture methods, which can maintain a stable culture environment, provide continuous nutrition and remove waste from the culture chamber. However, microfluidic systems mainly employ pump-driven systems or gravity-driven systems; although pump-driven microfluidics have high performance, including accurate flow rates, rapid media changes, high shear stress, and high pressure, they are expensive and complex and may require specialized manufacturing; however, such systems are not suitable for routine stem cell experiments, as these require portability, multi-condition setup flexibility and long-term stability; the microfluidics should be portable, movable to a clean bench, medium change without microbial contamination, and move to CO 2 In an incubator, cells are kept under the same conditions in vivo and moved to microscopic viewing conditions; maintaining a microfluidic system using pumps and peripheral tubes in a closed and sterile system may make the experiment more readyDifficult and complex. Thus, pump drive systems lack portability, flexibility, and stability.
While gravity driven systems, while having some portability and flexibility, are not suitable for long term culture because the flow of culture fluid is slowed down over several hours. The time required for the culture of induced pluripotent stem cells is relatively long, and it often takes a day to several days, and thus, the gravity-driven system is not suitable for the culture of induced pluripotent stem cells.
Disclosure of Invention
The technical problems to be solved by the utility model are as follows: the induced pluripotent stem cell culture device overcomes the defects of the prior art, can be suitable for stem cell culture without high shear stress, and solves the culture problem of induced pluripotent stem cells.
In order to solve the technical problems, the technical scheme of the utility model is as follows:
the induced pluripotent stem cell culture device comprises a microfluidic culture device arranged on a workbench, wherein the microfluidic culture device comprises a main channel communicated with a culture medium output tank and a culture medium receiving tank, a plurality of culture chambers are arranged on the main channel, each culture chamber is a cylindrical chamber, and a cell inlet and a cell outlet are respectively arranged outwards from arc tops on two sides of the culture chamber, which are perpendicular to the main channel; the workbench comprises a table top and a driving box body which is hinged with the table top, one end of the table top is hinged with the driving box body through a fixed shaft, the other end of the table top is hinged with the driving box body through a sleeving device, and the middle deflection sleeving device end of the table top is hinged with a driving rod of a driving device arranged in the driving box body through a supporting rod; the top of the driving box body is provided with a hollowed-out groove which is matched with the driving rod running track; the bottom of the table top is also provided with an inclination sensor, and the inclination sensor and the driving device are electrically connected to an automatic control system.
Preferably, the microfluidic culture device is placed on the surface of a workbench.
Preferably, four culture chambers are arranged on the main channel, and the tops of the culture chambers are respectively provided with an adaptive top cover.
Preferably, a culture plate is arranged in the culture chamber. The culture plate is a PDMS (polydimethylsiloxane) culture plate.
Furthermore, the culture plate is a 96-hole culture plate, and the side walls of the holes are respectively provided with openings (which are convenient for culture medium circulation).
Preferably, the sleeving device comprises a sleeve, one end of the sleeve is sealed, the other end of the sleeve is connected with a sliding shaft in a sliding manner, and the other end of the sliding shaft is hinged to one end of the table top; the sealing end of the sleeve is hinged to the top of the driving box body; a damping spring is arranged in the sleeve.
Preferably, the driving device comprises a forward and reverse rotating motor, the forward and reverse rotating motor is connected with a screw rod (for driving the screw rod to rotate) through a coupler, a transmission block is connected to the screw rod in a threaded manner, and a driving rod is arranged at the upper part of the transmission block; the forward and reverse rotation motor is electrically connected to the automatic control system.
Preferably, the inclination sensor is an inclination sensor.
Preferably, the automatic control system is a PLC automatic control system, the automatic control system is provided with a control panel, and the control panel is arranged on the side wall of the driving box body.
Due to the adoption of the technical scheme, the utility model has the beneficial effects that:
according to the utility model, the table top is controlled by the workbench to incline slowly, so that the culture medium in the culture medium output tank flows into the culture medium receiving tank slowly, and the culture medium passes through the culture chamber in the flowing process to update the culture medium on the culture plate attached with the cells to be cultured, which is arranged in the culture chamber, so that the culture medium does not generate high shearing force, can continuously provide fresh culture medium, and solves the culture problem of the existing induced pluripotent stem cells.
Drawings
FIG. 1 is a schematic diagram of the structure of the present utility model;
FIG. 2 is a schematic top view of a microfluidic culture device according to the utility model;
in the figure, 1, a culture medium output tank; 2. a medium receiving tank; 3. a main channel; 4. a culture chamber; 5. a cell inlet; 6. a cell outlet; 7. a table top; 8. driving the box body; 9. a support rod; 10. a driving rod; 11. an inclination sensor; 12. a sleeve; 13. a slide shaft; 14. a damping spring; 15. a forward and reverse rotation motor; 16. a screw rod; 17. and a transmission block.
Detailed Description
The utility model is further illustrated in the following, in conjunction with the accompanying drawings and examples.
Embodiment one:
as shown in fig. 1 and 2, an induced pluripotent stem cell culture apparatus comprises a microfluidic culture apparatus (not shown) arranged on a workbench (not shown), wherein the microfluidic culture apparatus comprises a main channel 3 which is communicated with a culture medium output tank 1 and a culture medium receiving tank 2, four culture chambers 4 are arranged on the main channel 3, each culture chamber 4 is a cylindrical chamber, and a cell inlet 5 and a cell outlet 6 are respectively arranged outwards from arc tops of two sides of the culture chamber 4 which are perpendicular to the main channel 3; the workbench comprises a table top 7 and a driving box body 8 which is hinged with the table top 7, one end of the table top 7 is hinged with the driving box body 8 through a fixed shaft, the other end of the table top 7 is hinged with the driving box body 8 through a sleeving device (not shown), the middle deflection sleeving device end of the table top 7 is hinged with a driving rod 10 of a driving device (not shown) arranged in the driving box body 8 through a supporting rod 9, and the top of the driving box body 8 is provided with a hollowed-out groove (not shown) which is matched with the running track of the driving rod 10; the bottom of the table top 7 is also provided with an inclination sensor 11, both the inclination sensor 11 and the drive means being electrically connected to an automatic control system (not shown).
The sleeving device comprises a sleeve 12, one section of the sleeve 12 is sealed, the other end of the sleeve is connected with a sliding shaft 13 in a sliding manner, and the other end of the sliding shaft 13 is hinged to one end of the table top 7; the sealed end of the sleeve 12 is hinged to the top of the drive box 8; a damping spring 14 is arranged in the sleeve 12; the driving device comprises a forward and reverse rotation motor 15, the forward and reverse rotation motor 15 is connected with a screw rod 16 through a coupler (not shown), a transmission block 17 is connected to the screw rod 16 in a threaded manner, and a driving rod 10 is arranged on the upper portion of the transmission block 17.
In practical use, taking culture of human induced pluripotent stem cells (human iPSC) as an example, firstly preparing a culture medium which is Dulbecco modified Eagle culture medium containing 10% fetal bovine serum and 1% penicillin-streptomycin; pretreatment of human iPSC was conventionalAfter treatment in this manner, the plates coated with the human iPSC to be cultivated (suspended in AK02N broth) are placed in the respective cultivation chambers 4, and the microfluidic cultivation device is placed in CO 2 After the iPSC is attached to the surface of a culture plate in an incubator for 30 minutes, the micro-fluid culture device is taken out and placed on a table top 7 of a workbench, a culture medium is filled into a culture medium output tank 1, a driving device is started, the end of the culture medium output tank 1 is slowly lifted up (the lifting angle per day is not more than seven degrees) according to a set speed, the culture medium in the culture medium output tank 1 slowly flows into a culture medium receiving tank 2 through a main channel 3 and a culture chamber 4, so that the human iPSC on the culture plate in the culture chamber 4 can obtain fresh culture medium at any time, and the influence of shearing force caused by liquid flow is avoided, and the smooth culture is ensured. After 2-3 days of culture, the culture plate is taken out for detection, and the culture is stopped when the cell growth reaches the requirement, so that the primary culture process is completed.
It is to be understood that these examples are illustrative of the present utility model and are not intended to limit the scope of the present utility model. Further, it is understood that various changes and modifications may be made by those skilled in the art after reading the teachings of the present utility model, and such equivalents are intended to fall within the scope of the claims appended hereto.
Claims (6)
1. An induced pluripotent stem cell culture device, characterized in that: the micro-fluid culture device comprises a main channel which is communicated with a culture medium output tank and a culture medium receiving tank, wherein the main channel is provided with a plurality of culture chambers, each culture chamber is a cylindrical chamber, and a cell inlet and a cell outlet are respectively arranged outwards from arc tops at two sides of the culture chamber which are vertical to the main channel; the workbench comprises a table top and a driving box body which is hinged with the table top, one end of the table top is hinged with the driving box body through a fixed shaft, the other end of the table top is hinged with the driving box body through a sleeving device, and the middle deflection sleeving device end of the table top is hinged with a driving rod of a driving device arranged in the driving box body through a supporting rod; the top of the driving box body is provided with a hollowed-out groove matched with the driving rod running track; and the bottom of the table top is also provided with an inclination sensor, and the inclination sensor and the driving device are electrically connected to an automatic control system.
2. The induced pluripotent stem cell culture apparatus of claim 1, wherein: the microfluidic culture device is placed on the top of a workbench.
3. The induced pluripotent stem cell culture apparatus of claim 1, wherein: four culture chambers are arranged on the main channel, and the tops of the culture chambers are respectively provided with an adaptive top cover.
4. The induced pluripotent stem cell culture apparatus of claim 1, wherein: a culture plate is arranged in the culture chamber.
5. The induced pluripotent stem cell culture apparatus of claim 1, wherein: the sleeving device comprises a sleeve, one end of the sleeve is sealed, the other end of the sleeve is connected with a sliding shaft in a sliding manner, and the other end of the sliding shaft is hinged to one end of the table top; the sealing end of the sleeve is hinged to the top of the driving box body; and a damping spring is arranged in the sleeve.
6. The induced pluripotent stem cell culture apparatus of claim 1, wherein: the driving device comprises a forward and reverse rotating motor, the forward and reverse rotating motor is connected with a screw rod through a coupling, a transmission block is connected to the screw rod in a threaded manner, and a driving rod is arranged on the upper portion of the transmission block.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202321745014.7U CN220246140U (en) | 2023-07-05 | 2023-07-05 | Induced pluripotent stem cell culture device |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202321745014.7U CN220246140U (en) | 2023-07-05 | 2023-07-05 | Induced pluripotent stem cell culture device |
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| Publication Number | Publication Date |
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| CN220246140U true CN220246140U (en) | 2023-12-26 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN202321745014.7U Active CN220246140U (en) | 2023-07-05 | 2023-07-05 | Induced pluripotent stem cell culture device |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN117683634A (en) * | 2024-01-31 | 2024-03-12 | 山东格林医学科技有限公司 | Stem cell culture device and method for diabetes treatment |
-
2023
- 2023-07-05 CN CN202321745014.7U patent/CN220246140U/en active Active
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN117683634A (en) * | 2024-01-31 | 2024-03-12 | 山东格林医学科技有限公司 | Stem cell culture device and method for diabetes treatment |
| CN117683634B (en) * | 2024-01-31 | 2024-05-07 | 山东格林医学科技有限公司 | Stem cell culture device and method for diabetes treatment |
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