CN220166152U - Culture apparatus is used in preparation of glutamine transaminase - Google Patents
Culture apparatus is used in preparation of glutamine transaminase Download PDFInfo
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- CN220166152U CN220166152U CN202321617739.8U CN202321617739U CN220166152U CN 220166152 U CN220166152 U CN 220166152U CN 202321617739 U CN202321617739 U CN 202321617739U CN 220166152 U CN220166152 U CN 220166152U
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- stirring
- glutamine transaminase
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- 108010010779 glutamine-pyruvate aminotransferase Proteins 0.000 title claims abstract description 32
- 238000002360 preparation method Methods 0.000 title claims description 8
- 238000003756 stirring Methods 0.000 claims abstract description 46
- 238000010438 heat treatment Methods 0.000 claims abstract description 25
- 238000007790 scraping Methods 0.000 claims abstract description 12
- 230000010261 cell growth Effects 0.000 abstract description 7
- 230000019522 cellular metabolic process Effects 0.000 abstract description 7
- 235000015097 nutrients Nutrition 0.000 abstract description 7
- 239000000126 substance Substances 0.000 abstract description 4
- 238000012258 culturing Methods 0.000 abstract 1
- 230000000694 effects Effects 0.000 description 3
- 239000007788 liquid Substances 0.000 description 3
- 238000012545 processing Methods 0.000 description 3
- 102000004190 Enzymes Human genes 0.000 description 2
- 108090000790 Enzymes Proteins 0.000 description 2
- 125000002485 formyl group Chemical group [H]C(*)=O 0.000 description 2
- 238000000034 method Methods 0.000 description 2
- 238000002156 mixing Methods 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 238000012544 monitoring process Methods 0.000 description 2
- 102000003839 Human Proteins Human genes 0.000 description 1
- 108090000144 Human Proteins Proteins 0.000 description 1
- CKLJMWTZIZZHCS-REOHCLBHSA-N L-aspartic acid Chemical compound OC(=O)[C@@H](N)CC(O)=O CKLJMWTZIZZHCS-REOHCLBHSA-N 0.000 description 1
- KSMRODHGGIIXDV-YFKPBYRVSA-N N-acetyl-L-glutamine Chemical compound CC(=O)N[C@H](C(O)=O)CCC(N)=O KSMRODHGGIIXDV-YFKPBYRVSA-N 0.000 description 1
- 125000002777 acetyl group Chemical group [H]C([H])([H])C(*)=O 0.000 description 1
- 238000013019 agitation Methods 0.000 description 1
- 235000003704 aspartic acid Nutrition 0.000 description 1
- 238000003556 assay Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- OQFSQFPPLPISGP-UHFFFAOYSA-N beta-carboxyaspartic acid Natural products OC(=O)C(N)C(C(O)=O)C(O)=O OQFSQFPPLPISGP-UHFFFAOYSA-N 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 230000037149 energy metabolism Effects 0.000 description 1
- 239000003292 glue Substances 0.000 description 1
- ZDXPYRJPNDTMRX-UHFFFAOYSA-N glutamine Natural products OC(=O)C(N)CCC(N)=O ZDXPYRJPNDTMRX-UHFFFAOYSA-N 0.000 description 1
- 238000011534 incubation Methods 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 238000001243 protein synthesis Methods 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 230000014616 translation Effects 0.000 description 1
Landscapes
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The utility model is suitable for the technical field of culture devices, and provides a culture device for preparing glutamine transaminase, which comprises a heating component, a stirring component, a scraping component and a basic component, wherein the heating component comprises a threaded rod, the outer wall of the threaded rod is in threaded connection with a heating device, the top end of the threaded rod is fixedly connected with a secondary belt pulley, both sides of the outer wall of the threaded rod are respectively and rotatably connected with a limiting block, the stirring component comprises an L-shaped fixing plate, the top end of the L-shaped fixing plate is fixedly connected with a motor, the driving end of the motor is fixedly connected with a stirring shaft, the top of the outer wall of the stirring shaft is fixedly connected with a main belt pulley, the device solves the problem that during culturing of glutamine transaminase, stirring is lack, so that internal nutrient substances are unevenly distributed, thereby affecting growth and metabolism of cells, and achieving that the stirring shaft rotates with stirring blades, so that nutrient substances in a culture box are evenly distributed, and simultaneously, cell growth and metabolism can be promoted.
Description
Technical Field
The utility model relates to the technical field of culture devices, in particular to a culture device for preparing glutamine transaminase.
Background
Glutamine transaminase is an enzyme whose function is to convert glutamine to aspartic acid and formyl to N-acetylglutamine. In this process formyl groups are transferred to coa to form acetyl coa, and the presence of glutamine transaminase plays an important role in human protein synthesis and energy metabolism, an enzyme that is also commonly used in clinical diagnostic assays and bioscience research.
Currently, the culture devices on the market lack agitation in the cultivation of glutamine transaminase, resulting in uneven distribution of nutrients inside, thereby affecting the growth and metabolism of cells.
Disclosure of Invention
Aiming at the defects existing in the prior art, the utility model aims to provide the culture device for preparing the glutamine transaminase, which is used for enabling a stirring shaft to rotate along with stirring blades, so that nutrient substances in a culture box body are uniformly distributed, and meanwhile, the growth and metabolism of cells can be promoted, thereby being beneficial to the generation of the glutamine transaminase.
In order to achieve the above purpose, the present utility model provides the following technical solutions:
the culture device for preparing the glutamine transaminase comprises a heating component, a stirring component, a scraping component and a basic component, wherein the heating component comprises a threaded rod, the outer wall of the threaded rod is in threaded connection with the heating device, the top end of the threaded rod is fixedly connected with a secondary belt pulley, and two sides of the outer wall of the threaded rod are both rotationally connected with limiting blocks;
the stirring assembly comprises an L-shaped fixing plate, a motor is fixedly connected to the top end of the L-shaped fixing plate, a stirring shaft is fixedly connected to the driving end of the motor, a main belt pulley is fixedly connected to the top of the outer wall of the stirring shaft, and a plurality of stirring blades are fixedly connected to the outer wall of the stirring shaft;
the scraping assembly comprises a U-shaped rod, an electric push rod is fixedly connected to the middle of the inner wall of the top end of the U-shaped rod, and an annular scraping plate is fixedly connected to the bottom end of the U-shaped rod.
The utility model is further provided with: the rear end of the limiting block is fixedly connected to the front ends of the cover plate and the incubator body respectively, and the inner wall of the heating device is slidably connected to the outer wall of the incubator body.
The utility model is further provided with: the bottom end of the L-shaped fixing plate is fixedly connected to the top end of the cover plate, and the outer wall of the stirring shaft is rotationally connected to the inner wall of the cover plate corresponding to the lower side of the main belt pulley.
The utility model is further provided with: the electric putter bottom fixed connection is in apron top rear side, U-shaped pole outer wall sliding connection is in the apron bottom.
The utility model is further provided with: the outer wall of the annular scraping plate is arranged on the inner wall of the incubator body.
The utility model is further provided with: the main belt pulley is connected with the limiting block through a belt.
The utility model has the advantages that:
1. in the preparation process, a proper amount of liquid is required to be poured into the incubator body through the feeding pipe, at the moment, the motor is started to drive the stirring shaft to rotate, the stirring shaft drives the stirring blades to rotate, so that nutrient substances in the incubator body are uniformly distributed, and meanwhile, cell growth and metabolism can be promoted, so that glutamine transaminase generation is facilitated, the stirring shaft drives the main belt pulley to rotate during stirring, the main belt pulley drives the auxiliary belt pulley to rotate through the belt, the auxiliary belt pulley drives the threaded rod to rotate, and the heating device moves up and down due to the threaded effect of the threaded rod, and simultaneously, the inside of the incubator body is stirred, so that uniform heating can be performed in the incubator body.
2. By starting the electric push rod to move downwards along with the U-shaped rod, the U-shaped rod moves downwards along with the annular scraping plate, so that glutamine transaminase adhered on the inner wall of the incubator body is scraped, and the next preparation of glutamine transaminase is prevented from being influenced.
Drawings
FIG. 1 is a top perspective view of the right front side of a culture apparatus for producing glutamine transaminase of the present utility model;
FIG. 2 is a schematic perspective view showing the sectional structure of an incubator body of the culture apparatus for producing glutamine transaminase of the present utility model;
FIG. 3 is a schematic perspective view showing a cross-sectional structure of a cover plate of a culture apparatus for producing glutamine transaminase of the present utility model;
in the figure: 1. a heating assembly; 101. a heating device; 102. a threaded rod; 103. a limiting block; 104. a secondary pulley; 2. a stirring assembly; 201. a main pulley; 202. an L-shaped fixing plate; 203. a motor; 204. stirring the leaves; 205. a stirring shaft; 3. a scraping assembly; 301. a U-shaped rod; 302. an electric push rod; 303. an annular scraping plate; 4. a base assembly; 401. a culture box body; 402. a cover plate; 403. a temperature sensor; 404. a control panel; 405. and feeding the material pipe.
Detailed Description
It should be noted that, without conflict, the embodiments of the present utility model and features of the embodiments may be combined with each other. The utility model will be described in detail below with reference to the drawings in connection with embodiments.
It is noted that all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this utility model belongs unless otherwise indicated.
In the present utility model, unless otherwise indicated, the terms "upper" and "lower" are used generally with respect to the directions shown in the drawings, or with respect to the vertical, vertical or gravitational directions; also, for ease of understanding and description, "left, right" is generally directed to the left, right as shown in the drawings; "inner and outer" refer to inner and outer relative to the outline of the components themselves, but the above-described orientation terms are not intended to limit the present utility model.
Referring to fig. 1-3, the present utility model provides the following technical solutions:
the culture device for preparing the glutamine transaminase comprises a heating component 1, a stirring component 2, a scraping component 3 and a basic component 4, wherein the heating component 1 comprises a threaded rod 102, the outer wall of the threaded rod 102 is in threaded connection with a heating device 101, the top end of the threaded rod 102 is fixedly connected with a secondary belt pulley 104, and two sides of the outer wall of the threaded rod 102 are both in rotary connection with limiting blocks 103;
the stirring assembly 2 comprises an L-shaped fixing plate 202, a motor 203 is fixedly connected to the top end of the L-shaped fixing plate 202, a stirring shaft 205 is fixedly connected to the driving end of the motor 203, a main belt pulley 201 is fixedly connected to the top of the outer wall of the stirring shaft 205, and a plurality of stirring blades 204 are fixedly connected to the outer wall of the stirring shaft 205;
the basic assembly 4 comprises an incubator body 401, a cover plate 402 is fixedly connected to the top end of the incubator body 401, a temperature sensor 403 is fixedly connected to the right side of the top end of the cover plate 402, a control panel 404 is fixedly connected to the front side of the top end of the cover plate 402, corresponding to the temperature sensor 403, and a feeding pipe 405 is fixedly connected to the left side of the top end of the cover plate 402.
In addition, in the preparation process, a proper amount of liquid needs to be poured into the incubator 401 through the feeding pipe 405, at this time, the motor 203 is started to rotate with the stirring shaft 205, the stirring shaft 205 is started to rotate with the stirring blade 204, so that nutrients in the incubator 401 are uniformly distributed, and meanwhile, cell growth and metabolism can be promoted, so that glutamine transaminase generation is facilitated, in the stirring process, the stirring shaft 205 is started to rotate with the main belt pulley 201, the main belt pulley 201 is started to rotate with the auxiliary belt pulley 104 through the belt, the auxiliary belt pulley 104 is started to rotate with the threaded rod 102, the heating device 101 moves up and down due to the threaded effect of the threaded rod 102, and meanwhile, the interior of the incubator 401 is stirred, so that uniform heating can be performed in the incubator 401, the temperature in the incubator 401 is detected through the temperature sensor 403 in the heating process, and the detected result is transmitted to the control panel 404 for real-time monitoring.
Further explaining, strike-off subassembly 3 includes U-shaped pole 301, and U-shaped pole 301 top inner wall middle part fixedly connected with electric putter 302, U-shaped pole 301 bottom fixedly connected with annular scraper blade 303, and through starting electric putter 302 and carrying U-shaped pole 301 to move down, U-shaped pole 301 carries annular scraper blade 303 to move down, strike off the glutamine transaminase that glues on the inner wall of incubator 401, avoid influencing the preparation of glutamine transaminase next time.
Further explaining, stopper 103 rear end fixed connection is at apron 402 and incubator body 401 front end respectively, carry out spacing processing through apron 402 and incubator body 401 to stopper 103, heating device 101 inner wall sliding connection is at incubator body 401 outer wall, carry out spacing to heating device 101 through incubator body 401, L shape fixed plate 202 bottom fixed connection is on the apron 402 top, L shape fixed plate 202 and apron 402 are together fixed, the downside rotation that the (mixing) shaft 205 outer wall corresponds main belt pulley 201 is connected at the apron 402 inner wall, the apron 402 carries out spacing processing to the (mixing) shaft 205), electric putter 302 bottom fixed connection is on apron 402 top rear side, apron 402 carries out spacing processing to electric putter 302, U-shaped pole 301 outer wall sliding connection is in the apron 402 bottom, annular scraper blade 303 outer wall sets up at incubator body 401 inner wall, main belt pulley 201 and stopper 103 pass through the belt and link to each other.
The working principle of the culture device for preparing glutamine transaminase provided by the utility model is as follows: firstly, a proper amount of liquid needs to be poured into the incubator 401 through the feeding pipe 405 in the preparation process, at this time, the motor 203 is started to rotate with the stirring shaft 205, the stirring shaft 205 is started to rotate with the stirring blade 204, so that nutrients in the incubator 401 are uniformly distributed, and meanwhile, cell growth and metabolism can be promoted, so that glutamine transaminase generation is facilitated, the stirring shaft 205 is started to rotate with the main belt pulley 201, the main belt pulley 201 is started to rotate with the auxiliary belt pulley 104 through the belt, the auxiliary belt pulley 104 is started to rotate with the threaded rod 102, the heating device 101 moves up and down due to the threaded effect of the threaded rod 102, meanwhile, the interior of the incubator 401 is stirred, so that the interior of the incubator 401 can be uniformly heated, the temperature in the incubator 401 is detected through the temperature sensor 403 in the heating process, the detected result is transmitted to the control panel 404, real-time monitoring is performed, after the incubation is finished, the electric push rod 302 is started to move downwards with the U-shaped rod 301 to move downwards, the annular scraper 303 is started, and glutamine transaminase adhered on the inner wall of the incubator 401 is scraped down, so that the glutamine transaminase is prevented from being prepared.
It will be apparent that the embodiments described above are merely some, but not all, embodiments of the utility model. All other embodiments, which can be made by those skilled in the art based on the embodiments of the present utility model without making any inventive effort, shall fall within the scope of the present utility model.
It is noted that the terminology used herein is for the purpose of describing particular embodiments only and is not intended to be limiting of exemplary embodiments according to the present utility model. As used herein, the singular is also intended to include the plural unless the context clearly indicates otherwise, and furthermore, it is to be understood that the terms "comprises" and/or "comprising" when used in this specification are taken to specify the presence of stated features, steps, operations, devices, components, and/or combinations thereof.
It should be noted that the terms "first," "second," and the like in the description and the claims of the present utility model and the above figures are used for distinguishing between similar objects and not necessarily for describing a particular sequential or chronological order. It is to be understood that the data so used may be interchanged where appropriate such that embodiments of the utility model described herein may be implemented in sequences other than those illustrated or otherwise described herein.
The above description is only of the preferred embodiments of the present utility model and is not intended to limit the present utility model, but various modifications and variations can be made to the present utility model by those skilled in the art. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present utility model should be included in the protection scope of the present utility model.
Claims (7)
1. The utility model provides a glutamine transaminase preparation is with culture apparatus, includes heating element (1), stirring element (2), strike-off subassembly (3) and basic component (4), its characterized in that: the heating assembly (1) comprises a threaded rod (102), the outer wall of the threaded rod (102) is in threaded connection with a heating device (101), the top end of the threaded rod (102) is fixedly connected with an auxiliary belt pulley (104), and two sides of the outer wall of the threaded rod (102) are both rotationally connected with limiting blocks (103);
the stirring assembly (2) comprises an L-shaped fixing plate (202), a motor (203) is fixedly connected to the top end of the L-shaped fixing plate (202), a stirring shaft (205) is fixedly connected to the driving end of the motor (203), a main belt pulley (201) is fixedly connected to the top of the outer wall of the stirring shaft (205), and a plurality of stirring blades (204) are fixedly connected to the outer wall of the stirring shaft (205);
the scraping assembly (3) comprises a U-shaped rod (301), an electric push rod (302) is fixedly connected to the middle of the inner wall of the top end of the U-shaped rod (301), and an annular scraping plate (303) is fixedly connected to the bottom end of the U-shaped rod (301).
2. The culture apparatus for producing glutamine transaminase according to claim 1, wherein: the basic assembly (4) comprises an incubator body (401), a cover plate (402) is fixedly connected to the top end of the incubator body (401), a temperature sensor (403) is fixedly connected to the right side of the top end of the cover plate (402), a control panel (404) is fixedly connected to the front side of the top end of the cover plate (402) corresponding to the temperature sensor (403), and a feeding pipe (405) is fixedly connected to the left side of the top end of the cover plate (402).
3. The culture apparatus for producing glutamine transaminase according to claim 1, wherein: the rear end of the limiting block (103) is fixedly connected to the front ends of the cover plate (402) and the incubator body (401) respectively, and the inner wall of the heating device (101) is slidably connected to the outer wall of the incubator body (401).
4. The culture apparatus for producing glutamine transaminase according to claim 1, wherein: the bottom end of the L-shaped fixing plate (202) is fixedly connected to the top end of the cover plate (402), and the outer wall of the stirring shaft (205) is rotatably connected to the inner wall of the cover plate (402) corresponding to the lower side of the main belt pulley (201).
5. The culture apparatus for producing glutamine transaminase according to claim 1, wherein: the bottom end of the electric push rod (302) is fixedly connected to the rear side of the top end of the cover plate (402), and the outer wall of the U-shaped rod (301) is slidably connected to the bottom end of the cover plate (402).
6. The culture apparatus for producing glutamine transaminase according to claim 1, wherein: the outer wall of the annular scraping plate (303) is arranged on the inner wall of the incubator body (401).
7. The culture apparatus for producing glutamine transaminase according to claim 1, wherein: the main belt pulley (201) is connected with the limiting block (103) through a belt.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202321617739.8U CN220166152U (en) | 2023-06-26 | 2023-06-26 | Culture apparatus is used in preparation of glutamine transaminase |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202321617739.8U CN220166152U (en) | 2023-06-26 | 2023-06-26 | Culture apparatus is used in preparation of glutamine transaminase |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN220166152U true CN220166152U (en) | 2023-12-12 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202321617739.8U Active CN220166152U (en) | 2023-06-26 | 2023-06-26 | Culture apparatus is used in preparation of glutamine transaminase |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN220166152U (en) |
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2023
- 2023-06-26 CN CN202321617739.8U patent/CN220166152U/en active Active
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