CN220132223U

CN220132223U - Protoplast separator

Info

Publication number: CN220132223U
Application number: CN202321612554.8U
Authority: CN
Inventors: 李婷怡; 马福利; 孙俊; 孙其宝; 周军永; 陆丽娟
Original assignee: Anhui Agricultural University AHAU; Institute of Gardening of Anhui Academy Agricultural Sciences
Current assignee: Anhui Agricultural University AHAU; Institute of Gardening of Anhui Academy Agricultural Sciences
Priority date: 2023-06-25
Filing date: 2023-06-25
Publication date: 2023-12-05
Anticipated expiration: 2033-06-25

Abstract

The utility model is applicable to the technical field of cell treatment, and provides a protoplast separator which comprises a box body, wherein a reaction tank is rotatably arranged in an inner cavity of the upper part of the box body in a supporting way; the reaction tank is provided with an opening; a stirring assembly is also arranged in the reaction tank; the lower inner cavity of the tank body is a waste liquid collecting cavity, a shape recovery tank is fixedly supported in the waste liquid collecting cavity, and an opening of the shape recovery tank is positioned right below the reaction tank; one end of the inner cavity of the reaction tank is also provided with a microporous filter screen, and the bottom of the reaction tank is provided with a second discharge outlet corresponding to the microporous filter screen. The protoplast separator provided by the utility model is convenient for separating the plasma wall of plant cells, and has good separation effect and convenient operation.

Description

Protoplast separator

Technical Field

The utility model belongs to the technical field of cell treatment, and particularly relates to a protoplast separator.

Background

Plant cell protoplasts are protoplasts obtained by removing plant cell walls and holding the protoplasts in a suitable permeate.

Since plant cell protoplasts contain very abundant plant proteins and can increase the nutrition of the product, the plant cell protoplasts are widely used as products processed from plants, and are applied to various foods, feeds, medicinal materials, nourishments and the like, and the cell walls of general plants contain cellulose and are very tough, so that the plant proteins in the protoplasts cannot be effectively utilized, and the cell walls need to be separated to obtain the plant cell protoplasts capable of increasing the nutrition of the product.

Disclosure of Invention

An object of an embodiment of the present utility model is to provide a protoplast separator, which aims to solve the technical problems set forth in the background art.

The embodiment of the utility model is realized in such a way that the protoplast separator comprises a box body, wherein a reaction tank is rotatably arranged in an inner cavity of the upper part of the box body in a supporting way;

the reaction tank is provided with an opening;

a stirring assembly is also arranged in the reaction tank;

the lower inner cavity of the tank body is a waste liquid collecting cavity, a shape recovery tank is fixedly supported in the waste liquid collecting cavity, and an opening of the shape recovery tank is positioned right below the reaction tank;

a microporous filter screen is further arranged at one end of the inner cavity of the reaction tank, and a second discharge port corresponding to the microporous filter screen is arranged at the bottom of the reaction tank;

the box body is also provided with a second motor for driving the reaction tank to rotate.

Further, a raw material inlet and a treatment fluid inlet are formed in the top plate of the box body, and the raw material inlet and the treatment fluid inlet are located above the opening.

Further, stirring subassembly is including rotating the puddler of locating in the retort, stirring subassembly is still including being used for the drive the rotatory first motor of puddler, first motor is installed on the box, the coaxial support rotation of output shaft of first motor stretches into in the retort, and be located first motor output shaft in the retort with puddler fixed connection.

Further, the output shaft of the second motor is coaxially connected with the end part of the reaction tank, so that the reaction tank can rotate for a certain angle, the opening of the reaction tank faces downwards, and the flat plant cell solution in the reaction tank falls into the form recovery box.

Further, the second discharge port is offset from the opening of the form recovery tank so that the waste liquid discharged through the second discharge port falls directly into the waste liquid collection chamber.

Further, a third discharge port positioned right above the opening of the form recovery box is also arranged at the bottom of the reaction tank, and a valve is also arranged on the third discharge port, so that the opening of the reaction tank is downwards aligned to the opening of the form recovery box by utilizing the overturning of the reaction tank, and the flat plant cell solution in the reaction tank can fall into the form recovery box conveniently; the residual cell solution in the reaction tank can be discharged into the morphology recovery tank by opening the valve of the third discharge port.

Furthermore, the tank body is also provided with a clear water tank, the clear water tank is connected with a water injection pipe, and the other end of the water injection pipe stretches into the form recovery tank to supplement clear water into the form recovery tank.

Further, a first exhaust port connected with the waste liquid collecting cavity is arranged on one side of the bottom of the box body, and a valve is also arranged on the first exhaust port.

Further, a discharge pipe is arranged at the bottom of the box body, one end of the discharge pipe is connected with the shape recovery box and is used for discharging the cells which are full after infiltration recovery in the shape recovery box; the other end of the discharge pipe extends out of the box body; the discharge pipe is also provided with a discharge valve.

Further, a control panel is further arranged on the outer wall of the box body, and the control panel is electrically connected with the first motor and the second motor and used for controlling the starting of the first motor and the second motor.

Compared with the prior art, the protoplast separator provided by the utility model has the following technical advantages:

firstly, separating the cytoplasm of the plant cells by the solution in the reaction tank with the concentration higher than that of cytoplasm, and degrading the cell walls by the cellulase to leave flat plant cells without the cell walls;

secondly, clear water is stored in the form recovery box, so that after the flat plant cells subjected to the plasma wall separation in the reaction tank enter the form recovery box, the cells are recovered to be full through the penetration of the clear water.

Drawings

FIG. 1 is a view in the tank of a protoplast separator according to the utility model;

FIG. 2 is an external view of a tank of a protoplast separator according to the utility model;

FIG. 3 is a schematic structural view of a reaction tank in the protoplast separator according to the utility model;

fig. 4 is a schematic structural diagram of a morphological recovery box in a protoplast separator according to the utility model.

In fig. 1-4: 100. a case; 101. a raw material inlet; 102. a treatment fluid inlet; 103. a support leg; 104. a first discharge port; 105. a waste liquid collection chamber; 200. a control panel; 300. a reaction tank; 301. a first motor; 302. a second discharge port; 303. a third discharge port; 304. a second motor; 305. a stirring rod; 306. a microporous filter screen; 400. a shape recovery box; 401. a discharge pipe; 402. a discharge valve; 500. a clean water tank; 501. and a water injection pipe.

Detailed Description

The present utility model will be described in further detail with reference to the drawings and examples, in order to make the objects, technical solutions and advantages of the present utility model more apparent. It should be understood that the specific embodiments described herein are for purposes of illustration only and are not intended to limit the scope of the utility model.

Specific implementations of the utility model are described in detail below in connection with specific embodiments.

As shown in fig. 1-2, in the embodiment of the present utility model, a protoplast separator comprises a tank 100, a reaction tank 300 is rotatably supported in an upper inner cavity of the tank 100, an opening is formed on the reaction tank 300, a raw material inlet 101 and a treatment liquid inlet 102 are formed on a top plate of the tank 100, and the raw material inlet 101 and the treatment liquid inlet 102 are located above the opening, so that a plant cell solution and a treatment liquid to be treated can be added into the reaction tank 300 through the raw material inlet 101 and the treatment liquid inlet 102, and the treatment liquid comprises cellulase and a sodium chloride solution with a concentration of 1%.

Preferably, in the embodiment of the present utility model, a stirring assembly is further disposed in the reaction tank 300, and the stirring assembly is used for stirring and mixing the plant cell solution and the treatment solution, and the concentration of the solution in the reaction tank 300 is higher than that of cytoplasm to realize the separation of the cytoplasm of the plant cell, and then the cellulase degrades the cell wall thereof, so as to leave the flat plant cell without the cell wall.

In the embodiment of the present utility model, as shown in fig. 1 and 3, the stirring assembly includes a stirring rod 305 rotatably disposed in a reaction tank 300, the stirring assembly further includes a first motor 301 for driving the stirring rod 305 to rotate, the first motor 301 is mounted on the box 100, an output shaft of the first motor 301 is coaxially supported and rotatably extends into the reaction tank 300, and an output shaft of the first motor 301 disposed in the reaction tank 300 is fixedly connected with the stirring rod 305.

With continued reference to fig. 1 to fig. 4, in the embodiment of the present utility model, the lower cavity of the tank body 100 is a waste liquid collecting cavity 105, a shape recovery tank 400 is fixedly supported in the waste liquid collecting cavity 105, an opening of the shape recovery tank 400 is located directly below the reaction tank 300, and clear water is stored in the shape recovery tank 400, so that after the flat plant cells separated from the plasma wall in the reaction tank 300 enter the shape recovery tank 400, the cells are recovered to be full through infiltration of the clear water.

With continued reference to fig. 1 and fig. 3, in an embodiment of the present utility model, the case 100 is further provided with a second motor 304 for driving the reaction tank 300 to rotate, where an output shaft of the second motor 304 is coaxially connected to an end of the reaction tank 300, so that after the reaction tank 300 can rotate for a certain angle, an opening of the reaction tank 300 faces downward, so that the flat plant cell solution in the reaction tank 300 falls into the shape recovery box 400.

Further, as shown in fig. 1 and 3, in the embodiment of the present utility model, a micro-pore filter screen 306 is further disposed at one end of the inner cavity of the reaction tank 300, a second outlet 302 corresponding to the micro-pore filter screen 306 is disposed at the bottom of the reaction tank 300, a corresponding valve is disposed on the second outlet 302, and the disposed micro-pore filter screen 306 filters the solution in the reaction tank 300, so that the waste liquid is discharged through the second outlet 302 and falls into the waste liquid collecting cavity 105.

Preferably, in the embodiment of the present utility model, the second discharge port 302 is offset from the opening of the shape recovery tank 400, so that the waste liquid discharged through the second discharge port 302 falls directly into the waste liquid collection chamber 105.

With continued reference to fig. 1, in the embodiment of the present utility model, a third outlet 303 is further provided at the bottom of the reaction tank 300 and is located directly above the opening of the shape recovery tank 400, and a valve is also provided on the third outlet 303, so that when the reaction tank 300 is turned over, the opening of the reaction tank 300 is aligned downward to the opening of the shape recovery tank 400, and after the flat plant cell solution in the reaction tank 300 falls into the shape recovery tank 400, the flat plant cell solution is easily dropped into the shape recovery tank 400; the residual cell solution in the reaction tank 300 may be discharged into the morphology recovery tank 400 by opening a valve of the third discharge port 303.

As shown in fig. 1 and 2, in the embodiment of the present utility model, a clean water tank 500 is further disposed on the tank 100, a water injection pipe 501 is disposed on the clean water tank 500 in a connecting manner, and the other end of the water injection pipe 501 extends into the shape recovery tank 400, so as to supplement clean water in the shape recovery tank 400.

Further, in the embodiment of the present utility model, the four corners of the bottom of the box 100 are provided with the supporting legs 103;

a first discharge outlet 104 connected with the waste liquid collecting cavity 105 is arranged at one side of the bottom of the box body 100, and a valve is also arranged on the first discharge outlet 104;

a discharge pipe 401 is further disposed at the bottom of the case 100, and one end of the discharge pipe 401 is connected to the shape recovery case 400, so as to discharge the cells, which are recovered and filled by infiltration, in the shape recovery case 400; the other end of the discharge pipe 401 extends out of the box body 100; the discharge pipe 401 is further provided with a discharge valve 402.

Further, as shown in fig. 1, in the embodiment of the present utility model, a control panel 200 is further disposed on the outer wall of the case 100, and the control panel 200 is electrically connected to the first motor 301 and the second motor 304, so as to control the starting of the first motor 301 and the second motor 304.

Although embodiments of the utility model have been disclosed above, they are not limited to the use listed in the specification and embodiments. It can be applied to various fields suitable for the present utility model. Additional modifications will readily occur to those skilled in the art. Therefore, the utility model is not to be limited to the specific details and illustrations shown and described herein, without departing from the general concepts defined in the claims and their equivalents.

Claims

1. A protoplast separator comprising a tank (100);

a reaction tank (300) is rotatably supported in the inner cavity of the upper part of the box body (100);

the reaction tank (300) is provided with an opening;

a stirring assembly is further arranged in the reaction tank (300);

the lower inner cavity of the box body (100) is a waste liquid collecting cavity (105), a form recovery box (400) is fixedly supported in the waste liquid collecting cavity (105), and an opening of the form recovery box (400) is positioned right below the reaction tank (300);

one end of the inner cavity of the reaction tank (300) is also provided with a microporous filter screen (306), and the bottom of the reaction tank (300) is provided with a second discharge outlet (302) corresponding to the microporous filter screen (306).

2. A protoplast separator as claimed in claim 1, wherein a feed inlet (101) and a treatment fluid inlet (102) are provided in a ceiling of said tank (100), said feed inlet (101) and said treatment fluid inlet (102) being both located above said opening.

3. A protoplast separator as claimed in claim 2, wherein the agitator assembly comprises an agitator bar (305) rotatably arranged in the reaction tank (300), and wherein the agitator assembly further comprises a first motor (301) for driving the agitator bar (305) in rotation.

4. A protoplast separator as claimed in claim 3, wherein a second motor (304) for driving the reaction tank (300) to rotate is further provided on the box (100), and an output shaft of the second motor (304) is coaxially connected to an end of the reaction tank (300).

5. A protoplast separator as claimed in claim 3, wherein said second outlet (302) is offset from the opening of said shape recovery box (400).

6. A protoplast separator as claimed in claim 3 or 5, wherein the bottom of said reaction tank (300) is further provided with a third discharge port (303) located directly above the opening of said shape recovery tank (400).

7. The protoplast separator according to any of claims 2-5, wherein a clear water tank (500) is further provided on the tank body (100), a water injection pipe (501) is connected to the clear water tank (500), and the other end of the water injection pipe (501) extends into the shape recovery tank (400).

8. A protoplast separator as claimed in claim 7, wherein a bottom side of said box (100) is provided with a first discharge port (104) connected to said waste collection chamber (105).

9. The protoplast separator of claim 8, wherein a bottom of the box (100) is further provided with a discharge pipe (401), and wherein one end of the discharge pipe (401) is connected to the shape recovery box (400); the other end of the discharge pipe (401) extends out of the box body (100); the discharge pipe (401) is also provided with a discharge valve (402).

10. The protoplast separator as claimed in claim 4, wherein a control panel (200) is further provided on an outer wall of the box (100), and the control panel (200) is electrically connected to the first motor (301) and the second motor (304).