CN220012637U - Sampling device of fluorescence PCR appearance - Google Patents

Abstract

The utility model discloses a sampling device of a fluorescence PCR instrument, which relates to the technical field of sampling devices of fluorescence PCR instruments and comprises an outer tube, wherein a baffle is nested and installed on the outer wall of the outer tube, a piston rod is nested and installed on the top of the outer tube, a top plate is installed on the top of the piston rod, connecting rods are symmetrically installed at the bottom of the top plate and penetrate through the baffle, return springs are nested and installed on the outer wall of each connecting rod, one ends of the return springs are attached to the bottom of the top plate, the other ends of the return springs are attached to the top of the baffle, limiting blocks are fixedly installed at the bottom of each connecting rod, threaded holes are symmetrically formed in the bottom of the top plate, threaded rods are welded at the top of each connecting rod, and the threaded rods are in threaded connection with the threaded holes.

Description

Sampling device of fluorescence PCR appearance

Technical Field

The utility model relates to the technical field of sampling devices of fluorescent PCR instruments, in particular to a sampling device of a fluorescent PCR instrument.

Background

The real-time fluorescence quantitative PCR instrument consists of a fluorescence quantitative system and a computer, wherein the fluorescence quantitative system is used for monitoring fluorescence in a circulation process, the computer connected with real-time equipment is used for collecting fluorescence data, the data is displayed in a form of a chart through developed real-time analysis software, and the original data is plotted as a chart of fluorescence intensity relative to the circulation number. After the original data is collected, analysis can be started, software of the real-time equipment can enable the collected data to be subjected to normalization processing to make up for differences of background fluorescence, and after normalization, threshold level can be set, namely the level of fluorescence data is analyzed, and a PCR instrument needs to sample before sample detection.

Through retrieving, chinese patent grant number CN 214781850U discloses a sampling device for fluorescence quantitative PCR appearance, relate to fluorescence quantitative PCR appearance's sampling device technical field, concretely a sampling device for fluorescence quantitative PCR appearance, through sliding movable block in the sleeve outside, make movable block slide to corresponding scale position, then make movable block fixed in position through tightening the locating screw, thereby make movable rod can only upwards move the locating disk and support the movable block bottom, thereby control the distance that push-pull block drove the piston rod upwards pull, thereby can control device's sample extraction volume according to the scale, can prevent effectively that the extraction of sample is extravagant, current sampling device needs both hands to take at the in-process of taking a sample and takes a sample, such sampling method is comparatively troublesome.

Disclosure of Invention

Aiming at the defects of the prior art, the utility model provides a sampling device of a fluorescence PCR instrument, which solves the problem that the existing sampling device needs to be taken by two hands for sampling in the sampling process, and the sampling mode is troublesome.

In order to achieve the above purpose, the utility model is realized by the following technical scheme: the utility model provides a sampling device of fluorescence PCR appearance, includes the outer tube, the baffle is installed to outer tube outer wall nest, the piston rod is installed to outer tube top nest, the roof is installed at the piston rod top, the connecting rod is installed to roof bottom symmetry, the connecting rod runs through the baffle, reset spring is all installed in the equal nest of connecting rod outer wall.

Preferably, one end of the reset spring is attached to the bottom of the top plate, the other end of the reset spring is attached to the top of the baffle, and the bottom of the connecting rod is fixedly provided with a limiting block.

Preferably, the screw holes are symmetrically formed in the bottom of the top plate, the threaded rods are welded at the tops of the connecting rods, the threaded rods are connected with the screw holes in a threaded mode, the through holes are symmetrically formed in the tops of the baffles, the connecting rods penetrate through the through holes, and the diameter of the limiting block is larger than that of the through holes.

Preferably, the scale plate is installed to outer wall symmetry of outer tube, spacing hole has been seted up at the baffle top, the outer tube is installed to spacing hole inside gomphosis, spacing groove has been seted up to spacing hole both sides, spacing groove runs through the baffle.

Preferably, extension plates are fixedly installed on two sides of the baffle, a mounting hole is formed in the top of the outer tube, a piston rod is installed in the mounting hole in a jogged mode, the bottom of the outer tube is connected with a connecting tube in a threaded mode, and a needle is installed at the bottom of the connecting tube.

The utility model provides a sampling device of a fluorescence PCR instrument. The beneficial effects are as follows:

according to the sampling device of the fluorescent PCR instrument, the top plate and the piston rod can automatically move through the setting of the reset spring, so that sampling operation can be performed by only one hand in the sampling process, and the convenience of device use is greatly improved.

Drawings

FIG. 1 is a schematic view of the overall structure of the present utility model;

FIG. 2 is a schematic view of a baffle structure according to the present utility model;

FIG. 3 is a schematic view of the bottom structure of the top plate of the present utility model;

FIG. 4 is a schematic view of the structure of the outer tube of the present utility model.

In the figure, 1, an outer tube; 2. a piston rod; 3. a top plate; 4. a connecting rod; 5. a return spring; 6. a baffle; 7. an extension plate; 8. a limiting block; 9. a scale plate; 10. a connecting pipe; 11. a needle; 12. a through hole; 13. a limiting hole; 14. a limit groove; 15. a threaded hole; 16. a threaded rod; 17. and (5) mounting holes.

Detailed Description

The following description of the embodiments of the present utility model will be made clearly and completely with reference to the accompanying drawings, in which it is apparent that the embodiments described are only some embodiments of the present utility model, but not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the utility model without making any inventive effort, are intended to be within the scope of the utility model.

Referring to fig. 1-4, the embodiment of the present utility model provides a technical solution: the utility model provides a sampling device of fluorescence PCR appearance, includes outer tube 1, outer tube 1 outer wall nested installs baffle 6, outer tube 1 top nested installs piston rod 2, roof 3 is installed at piston rod 2 top, connecting rod 4 is installed to roof 3 bottom symmetry, connecting rod 4 runs through baffle 6, all nested reset spring 5 of installing of connecting rod 4 outer wall, reset spring 5's setting makes piston rod 2 and roof 3 can realize automatic re-setting.

The laminating of reset spring 5 one end is in roof 3 bottom, the laminating of reset spring 5 other end is at baffle 6 top, the equal fixed mounting of connecting rod 4 bottom has stopper 8, and roof 3 and baffle 6 set up and provide a support for reset spring 5, and the circumstances of the inside roll-off of through-hole 12 can be prevented to stopper 8's setting simultaneously connecting rod 4.

Screw holes 15 have been seted up to roof 3 bottom symmetry, threaded rod 16 has all been welded at connecting rod 4 top, threaded rod 16 threaded connection screw hole 15, through-hole 12 has been seted up to baffle 6 top symmetry, connecting rod 4 runs through-hole 12, stopper 8 diameter is greater than the diameter of through-hole 12, and threaded rod 16's installation has been made things convenient for in the setting of screw hole 15, and stopper 8 can play a spacing effect that stops simultaneously.

The scale plate 9 is symmetrically arranged on the outer wall of the outer tube 1, the limit hole 13 is formed in the top of the baffle plate 6, the outer tube 1 is arranged in the limit hole 13 in a jogged mode, the limit grooves 14 are formed in two sides of the limit hole 13, the limit grooves 14 penetrate through the baffle plate 6, scales are carved on the scale plate 9, the baffle plate 6 is conveniently arranged on the outer wall of the outer tube 1 due to the arrangement of the limit hole 13, and meanwhile the scale plate 9 penetrates through the baffle plate 6 due to the arrangement of the limit grooves 14.

The baffle 6 both sides are all fixed mounting has extension board 7, mounting hole 17 has been seted up at outer tube 1 top, the piston rod 2 is installed to the inside gomphosis of mounting hole 17, outer tube 1 bottom threaded connection connecting pipe 10, needle 11 is installed to connecting pipe 10 bottom, connecting pipe 10 threaded connection outer tube 1, can conveniently change connecting pipe 10 like this, and the setting of extension board 7 has made things convenient for operating personnel's hand.

Working principle: in the use, rotate baffle 6 at first and make the spacing groove 14 on the baffle 6 align with scale plate 9, then remove baffle 6 to the position that needs to extract the scale, then rotatory outer tube 1, make spacing groove 14 not align with scale plate 9, then index finger and the middle finger on one hand of operating personnel place respectively in baffle 6 both sides extension board 7 bottoms that set up, then push down roof 3 through the thumb, the in-process that roof 3 moved down drives bottom-mounted connecting rod 4 and moves down, then form the compression to reset spring 5, loosen the thumb of pressing roof 3 after squeezing suitable position, reset spring 5 jack-up roof 3 under the circumstances of deformation, roof 3 drives bottom-mounted piston rod 2 and moves up and accomplish the absorption to the liquid.

The utility model relates to a 1, an outer tube; 2. a piston rod; 3. a top plate; 4. a connecting rod; 5. a return spring; 6. a baffle; 7. an extension plate; 8. a limiting block; 9. a scale plate; 10. a connecting pipe; 11. a needle; 12. a through hole; 13. a limiting hole; 14. a limit groove; 15. a threaded hole; 16. a threaded rod; 17. the mounting holes and the components are all universal standard components or components known to the skilled person, and the structure and the principle of the mounting holes and the components are all known to the skilled person through technical manuals or through routine experimental methods.

While the fundamental and principal features of the utility model and advantages of the utility model have been shown and described, it will be apparent to those skilled in the art that the utility model is not limited to the details of the foregoing exemplary embodiments, but may be embodied in other specific forms without departing from the spirit or essential characteristics thereof. The present embodiments are, therefore, to be considered in all respects as illustrative and not restrictive, the scope of the utility model being indicated by the appended claims rather than by the foregoing description, and all changes which come within the meaning and range of equivalency of the claims are therefore intended to be embraced therein. Any reference sign in a claim should not be construed as limiting the claim concerned.

Furthermore, it should be understood that although the present disclosure describes embodiments, not every embodiment is provided with a separate embodiment, and that this description is provided for clarity only, and that the disclosure is not limited to the embodiments described in detail below, and that the embodiments described in the examples may be combined as appropriate to form other embodiments that will be apparent to those skilled in the art.

Claims (5)

1. The utility model provides a sampling device of fluorescence PCR appearance, includes outer tube (1), its characterized in that: the novel outer tube is characterized in that a baffle (6) is installed on the outer wall of the outer tube (1) in a nested mode, a piston rod (2) is installed on the top of the outer tube (1) in a nested mode, a top plate (3) is installed on the top of the piston rod (2), connecting rods (4) are symmetrically installed at the bottom of the top plate (3), the connecting rods (4) penetrate through the baffle (6), and reset springs (5) are installed on the outer wall of each connecting rod (4) in a nested mode.

2. The sampling device of a fluorescent PCR instrument of claim 1, wherein: one end of the reset spring (5) is attached to the bottom of the top plate (3), the other end of the reset spring (5) is attached to the top of the baffle plate (6), and the bottom of the connecting rod (4) is fixedly provided with a limiting block (8).

3. The sampling device of a fluorescent PCR instrument of claim 2, wherein: threaded holes (15) are symmetrically formed in the bottom of the top plate (3), threaded rods (16) are welded at the tops of the connecting rods (4), the threaded rods (16) are in threaded connection with the threaded holes (15), through holes (12) are symmetrically formed in the tops of the baffles (6), the connecting rods (4) penetrate through the through holes (12), and the diameter of the limiting block (8) is larger than that of the through holes (12).

4. The sampling device of a fluorescent PCR instrument of claim 1, wherein: the outer tube (1) outer wall symmetry is installed scale plate (9), spacing hole (13) have been seted up at baffle (6) top, outer tube (1) are installed to spacing hole (13) inside gomphosis, spacing groove (14) have been seted up to spacing hole (13) both sides, spacing groove (14) run through baffle (6).

5. The sampling device of a fluorescent PCR instrument of claim 1, wherein: the novel medical device is characterized in that extension plates (7) are fixedly mounted on two sides of the baffle (6), mounting holes (17) are formed in the top of the outer tube (1), piston rods (2) are mounted in the mounting holes (17) in a jogged mode, the bottoms of the outer tube (1) are connected with connecting tubes (10) through threads, and needles (11) are mounted at the bottoms of the connecting tubes (10).