CN219930081U - Disposable cell culture utensil and cell culture device - Google Patents

Abstract

The utility model discloses a disposable cell culture apparatus, which comprises a transfer container, a culture container and a pipeline for communicating the transfer container and the culture container, wherein the transfer container is provided with a ventilation connecting part for enabling the inside of the transfer container to be ventilated to generate positive pressure or negative pressure, and the pipeline is provided with a first butt joint part for connecting an initial container and a second butt joint part for connecting a culture solution container. The utility model also discloses a cell culture device, including installation panel and foretell disposable cell culture utensil, a plurality of installation department, a plurality of pinch valve and a plurality of solid pipe fitting on the installation panel, transfer container demountable installation is on the installation department, and the pipeline is fixed on solid pipe fitting, and the pinch valve presss from both sides to locate on corresponding pipe position. The disposable cell culture apparatus and the cell culture device have the advantages of avoiding cell damage in the feeding and conveying processes, greatly improving the cell yield and the cell activity, and the like.

Description

Disposable cell culture utensil and cell culture device

Technical Field

The utility model relates to the technical field of cell medicine preparation, in particular to a disposable cell culture device and a cell culture apparatus.

Background

Cells may be cultured under specific conditions in a laboratory or industrial setting for various purposes. Typically, cells are grown in a closed vessel and covered with a solution, also referred to as a culture medium, which may provide necessary nutrients and other supplements for cell growth. The means for cell culture include a flat circular dish or laboratory flask. As cells grow and proliferate, they consume nutrients in the cell culture broth and produce waste byproducts. For this reason, cell culture medium must be added periodically to allow the cells to continue to thrive.

In the process of culturing, cells are continuously amplified, and the culture medium is also continuously increased, so that the existing cell culture method needs to continuously replace a culture bottle with larger capacity so as to ensure that the cells have proper living space. And is usually manually operated during replacement, with the risk of contamination during operation. For example, repeatedly opening a cell culture system and moving cell culture vessels between laboratory stations may expose cells to contamination. Furthermore, each operation performed manually is expensive and is susceptible to contamination (or cell damage) because the operator does not follow the proper procedure. Furthermore, determining when to change media or when to passaging cells is typically performed according to a predetermined schedule, which may not be optimal. Compliance with the set schedule may result in additional (and possibly unnecessary) use of laminar flow hoods (which consume significant amounts of energy to operate and are therefore costly).

In order to solve the problem of easy contamination of manual operations, there is a conventional cell culture line in which cells are cultured in a culture vessel having a variable volume, for example, the line of application No. cn201710729873. X. The pipeline can be cultivated only by being installed under specific equipment, and the equipment effectively solves the problem of easy pollution in the process of manually replacing the cultivation container, but faces some new problems: 1. the peristaltic pump is adopted to easily damage cells in the process of conveying the cells, the peristaltic pump can squeeze the cells during conveying, deformation and rupture of the cells are easy to be caused, and the cell activity rate is greatly influenced; 2. the culture vessel of the pipeline can be used for centrifugation, which means that cell centrifugation and cell culture are carried out on the same equipment, however, cell centrifugation usually only needs a short process, cell culture needs a period of weeks or even months, and the equipment cannot be centrifuged in the process of cell culture, so that the utilization rate of the equipment is low.

Disclosure of Invention

The utility model aims to solve the technical problem of overcoming the defects of the prior art and providing a disposable cell culture device and a cell culture apparatus which can avoid cell damage in the feeding and conveying process and greatly improve the cell yield and the cell activity.

In order to solve the technical problems, the utility model adopts the following technical scheme:

the disposable cell culture apparatus comprises a transfer container, a culture container and a pipeline for communicating the transfer container and the culture container, wherein the transfer container is provided with a ventilation connecting part for enabling the inside of the transfer container to be ventilated to generate positive pressure or negative pressure, and the pipeline is provided with a first butt joint part for connecting an initial container and a second butt joint part for connecting a culture solution container.

As a further improvement of the above technical scheme:

the first butt joint portion is butt jointed with an initial container, and/or the second butt joint portion is butt jointed with a culture solution container.

The pipeline is provided with a third butt joint part for connecting the collecting container.

The third butt joint part is butt jointed with a collecting container.

The collection container is provided with a sampling interface and/or a first gas filter.

And a demagnetizing bead assembly is arranged on a pipeline for communicating the collecting container with the initial container.

The pipeline is provided with a fourth butt joint part for connecting the sampling container.

The fourth butt joint part is in butt joint with a sampling container.

A counter for counting is arranged between the culture container and the transfer container.

The pipeline is provided with a fifth butt joint part for connecting the waste liquid container.

The fifth butt joint part is butt-jointed with a waste liquid container.

A waste liquid pipeline for discharging waste liquid is arranged between the culture container and the transfer container.

The pipeline is connected with the bottom of the transfer container.

The pipeline is also connected with the top of the transfer container.

The ventilation connecting part is arranged on the side wall or the top wall of the transfer container.

The ventilation connecting part is connected with a positive-negative pressure device, the positive-negative pressure device comprises a second gas filter and a gas pipe, and the second gas filter is communicated with the transfer container through the gas pipe.

The transfer container is a hard container.

The pipeline is provided with a sixth butt joint part for leakage detection.

The culture vessel has at least two inner sides of different areas, which form respective culture surfaces for culturing different volumes of cell solution.

The utility model provides a cell culture device, includes installation panel and foretell disposable cell culture utensil, a plurality of installation department, a plurality of pinch valve and a plurality of solid pipe fitting on the installation panel, transfer container demountable installation is on the installation department, the pipeline is fixed on solid pipe fitting, the pinch valve presss from both sides to locate on the corresponding pipe position.

Compared with the prior art, the utility model has the advantages that:

when the disposable cell culture apparatus is used, the disposable cell culture apparatus is arranged on the installation panel, the pinch valve on the installation panel is clamped on the corresponding pipe position, and the on-off of the corresponding section of pipeline is controlled through the pinch valve. In the cell culture process, connecting an initial container containing cell liquid with a first butt joint part, connecting a culture liquid container containing culture liquid with a second butt joint part, and connecting a positive-negative pressure device with a ventilation connection part; the method comprises the steps of clamping and disconnecting pipelines between an initial container and a transfer container, between a culture solution container and the transfer container and between the culture solution container and the transfer container through a pinch valve, pumping air from the transfer container through a positive-negative pressure device to generate negative pressure, releasing and opening the pipelines between the initial container and the transfer container and/or between the culture solution container and the transfer container through the pinch valve, enabling cell solution in the initial container and/or culture solution in the culture solution container to enter the transfer container under the action of the negative pressure, clamping and disconnecting the pipelines between the initial container and the transfer container, between the culture solution container and the transfer container through the pinch valve, releasing and opening the pipelines between the transfer container and the culture container, inflating the transfer container through a positive-negative pressure device to generate positive pressure, and enabling materials in the transfer container to enter the culture container. Therefore, materials (cell liquid and culture liquid) do not directly pass through the positive and negative pressure device in the feeding process, are not subjected to repeated extrusion action of the positive and negative pressure device, avoid cell damage in the feeding and conveying process, and greatly improve the cell yield and the cell activity.

The cell culture device of the utility model has the advantages that the disposable cell culture device is detachably arranged on the mounting panel, and the disposable cell culture device is scrapped after being used. Therefore, the pinch valve is not disposable with the disposable cell culture apparatus, can be used for multiple times, and saves cost.

Drawings

FIG. 1 is a schematic diagram of the structure of a disposable cell culture apparatus of the present utility model.

FIG. 2 is a schematic view showing the structure of a culture vessel of the disposable cell culture vessel of the present utility model.

FIG. 3 is a view showing the state of use of the disposable cell culture apparatus of the present utility model.

FIG. 4 is a diagram showing the cooperation of the disposable cell culture apparatus of the present utility model with a peristaltic pump.

Fig. 5 is an enlarged view of fig. 4 a.

FIG. 6 is a packaging view of the disposable cell culture apparatus of the present utility model.

FIG. 7 is a schematic structural view of a packing bag of the disposable cell culture apparatus of the present utility model.

FIG. 8 is a side view of the structure of the cell culture apparatus of the utility model.

The reference numerals in the drawings denote:

1. a transfer container; 10. a pipeline; 101. a sixth butt joint part; 11. a ventilation connection; 12. packaging bags; 13. a peristaltic pump; 2. a culture vessel; 20. a waste liquid pipeline; 3. an initial container; 31. a first butt joint part; 4. a culture medium container; 41. a second butt joint part; 5. a collection container; 51. a third butt joint part; 52. a sampling interface; 53. a first gas filter; 54. a demagnetizing bead assembly; 6. a sampling container; 61. a fourth butt joint part; 7. positive and negative pressure devices; 70. an air pipe; 71. a second gas filter; 80. a counter; 9. installing a panel; 91. a mounting part; 92. a pinch valve; 93. and fixing the pipe fitting.

Detailed Description

The utility model will be described in further detail with reference to the drawings and the specific examples.

Embodiment one:

fig. 1 to 7 show an embodiment of the disposable cell culture vessel of the present utility model, which comprises a relay vessel 1, a culture vessel 2, a pipe 10 for connecting the relay vessel 1 and the culture vessel 2, the relay vessel 1 being provided with a ventilation connection part 11 for ventilating the inside thereof to generate positive pressure or negative pressure, the pipe 10 being provided with a first docking part 31 for connecting the initial vessel 3 and a second docking part 41 for connecting the culture vessel 4.

When in use, the disposable cell culture apparatus is arranged on the installation panel 9 shown in fig. 4, the pinch valve 92 on the installation panel 9 is clamped on the corresponding pipe position, and the on-off of the pipeline 10 of the corresponding section is controlled through the pinch valve 92. In the cell culture process, connecting an initial container 3 containing cell liquid with a first butt joint part 31, connecting a culture liquid container 4 containing culture liquid with a second butt joint part 41, and connecting a positive and negative pressure device 7 with an aeration connecting part 11 in a sterile environment; the pipe 10 between the initial container 3 and the transfer container 1, the culture solution container 4 and the transfer container 1, and the pipe 10 between the culture solution container 2 and the transfer container 1 are clamped and disconnected through the pipe clamping valve 92, then the transfer container 1 is pumped by the positive and negative pressure device 7 to generate negative pressure, then the pipe 10 between the initial container 3 and the transfer container 1 and/or between the culture solution container 4 and the transfer container 1 is released and opened through the pipe clamping valve 92, under the negative pressure effect, the cell solution in the initial container 3 and/or the culture solution in the culture solution container 4 enter the transfer container 1, then the pipe 10 between the initial container 3 and the transfer container 1, the culture solution container 4 and the transfer container 1 is clamped and disconnected through the pipe clamping valve 92, the pipe 10 between the transfer container 1 and the culture container 2 is released and opened, and then the transfer container 1 is inflated by the positive and negative pressure device 7 to generate positive pressure, so that the materials of the transfer container 1 enter the culture container 2. In this way, materials (cell liquid and culture liquid) do not directly pass through the positive and negative pressure device 7 in the feeding process, are not subjected to repeated extrusion action of the positive and negative pressure device 7, avoid cell damage in the feeding and conveying process, and greatly improve the cell yield and the cell activity.

In this embodiment, the first docking portion 31 is docked with the initial container 3, so that, in the subsequent use, only the cell liquid needs to be injected into the initial container 3, and the initial container 3 capable of docking with the first docking portion 31 does not need to be prepared; and/or, the second docking portion 41 is docked with the culture medium container 4, so that, at the time of subsequent use, only the culture medium needs to be injected into the culture medium container 4, and the culture medium container 4 capable of docking with the second docking portion 41 does not need to be prepared.

In this embodiment, the line 10 is provided with a third docking portion 51 for connecting the collection container 5.

When in use, the collecting container 5 is connected with the third butt joint part 51 under the sterile environment, so after the cell culture of the culture container 2 is completed, the pipeline 10 between the initial container 3 and the transfer container 1, the culture liquid container 4 and the transfer container 1, the pipeline 10 between the collecting container 5 and the transfer container 1, the pipeline 1 between the culture container 2 and the transfer container 1 are clamped and closed through the pipe clamping valve 92, then the negative pressure is generated by pumping the transfer container 1 through the positive and negative pressure device 7, then the pipeline 10 between the culture container 2 and the transfer container 1 is released and opened through the pipe clamping valve 92, the cell in the culture container 2 enters the transfer container 1 under the negative pressure, then the pipeline 10 between the culture container 2 and the transfer container 1 is clamped and disconnected through the pipe clamping valve 92, the pipeline 10 between the transfer container 1 and the collecting container 5 is released and opened, and then the transfer container 1 is inflated through the positive and negative pressure device 7 to generate positive pressure, so that the material of the transfer container 1 enters the collecting container 5. Therefore, materials (cell liquid and culture liquid) do not directly pass through the positive and negative pressure device 7 in the discharging process, are not subjected to repeated extrusion action of the positive and negative pressure device 7, avoid cell damage in the discharging and conveying process, and greatly improve the cell yield and the cell activity.

In the present embodiment, the third docking portion 51 is docked with the collection container 5. In this way, there is no need to prepare a collection container 5 which can be docked with the third docking portion 51 at a later time of use.

In this embodiment, the collection container 5 is provided with a sampling interface 52 and/or a first gas filter 53. Sampling detection is facilitated by the provision of sampling interface 52. By providing a first gas filter 53 for balancing the gas pressure in the collection vessel 5.

In this embodiment, the collection vessel 5 is provided with a demagnetizing bead assembly 54 on the pipe 10 communicating with the initial vessel 3. Since the beads are generally mixed in the cell liquid contained in the culture vessel 2, the beads need to be removed during discharging, and therefore, a degaussing bead assembly 54 is provided on the pipe 10 communicating the collection vessel 5 with the initial vessel 3 to remove the beads in the cells.

In this embodiment, the line 10 is provided with a fourth docking portion 61 for connecting the sampling vessel 6. In the culture process of the culture container 2, the sampling container 6 is in butt joint with the fourth butt joint part 61, and the intermittent time length is used for sampling and detecting the culture container 2, so that the effect of monitoring the culture condition is achieved.

In the present embodiment, the fourth docking portion 61 is docked with the sampling vessel 6. In this way, it is not necessary to prepare the sampling vessel 6 which can be docked with the fourth docking portion 61 at the time of subsequent use.

In this embodiment, a counter 80 for counting is provided between the culture vessel 2 and the relay vessel 1. During sampling of the sampling vessel 6, the number of cells passing through is counted by a counter 80 for real-time monitoring of the culture process.

In this embodiment, the pipeline 10 is provided with a fifth docking portion for connecting to a waste liquid container. And the waste liquid container is connected with the fifth butt joint part, so that the waste liquid container can be used for collecting waste liquid in and after the culture process.

In this embodiment, the fifth docking portion is docked with the waste liquid container. In this way, in the subsequent use, it is not necessary to prepare a waste liquid container which can be docked with the fifth docking portion. The waste liquid container can be replaced by the culture liquid container 4, because the residual culture liquid in the culture liquid container 4 can not be reused generally and can only be treated as waste liquid, thus the waste liquid container is not required to be reset, and the cost is saved. Meanwhile, the fifth docking portion may be replaced with the second docking portion 41.

In this embodiment, a waste liquid line 20 for discharging waste liquid is provided between the culture vessel 2 and the relay vessel 1. The waste liquid line 20 is used to discharge the supernatant in the culture vessel 2.

In this embodiment, the pipeline 10 is connected to the bottom of the transfer vessel 1.

In this embodiment, the pipeline 10 is also connected to the top of the transfer container 1.

The pipe 10 connects the top and bottom of the relay container 1, since the culture container 2 needs both liquid feed (cells flow from the initial container 3 into the culture container 2) and liquid discharge (cells flow from the culture container 2 into the collection container 5), when liquid feed, liquid enters the relay container 1 from the initial container 3 through the pipe 10 and the top of the relay container 1, then enters the culture container 2 through the bottom of the relay container 1 and the pipe 10, and when liquid discharge, liquid enters the relay container 1 from the culture container 2 through the pipe 10 and the top of the relay container 1, then enters the collection container 5 through the bottom of the relay container 1 and the pipe 10, so that liquid is prevented from entering from the bottom of the relay container 1, regardless of whether liquid feed or liquid discharge is performed from the top of the relay container 1. If the liquid from the culture vessel 2 enters from the bottom of the transfer vessel 1, a large amount of bubbles are finally sucked into the transfer vessel 1, and the bursting of the bubbles adversely affects the cells.

In this embodiment, the ventilation connection 11 is provided on a side wall or a top wall of the transfer container 1. Thus, during the positive pressure process, gas enters from the side wall or the top wall of the transfer container 1, for example, the ventilation connection part 11 is arranged at the bottom of the transfer container 1, and when the transfer container 1 is filled with liquid, the liquid can enter the positive-negative pressure device 7 and even leak through the ventilation connection part 11.

In the present embodiment, the ventilation connection portion 11 is connected with the positive-negative pressure device 7, and the positive-negative pressure device 7 includes a second gas filter 71 and a gas pipe 70, and the second gas filter 71 communicates with the relay container 1 through the gas pipe 70. When in use, the air pipe 70 is arranged in the peristaltic pump 13 (as shown in fig. 4 and 5), the peristaltic pump 13 can be ventilated or pumped to the transfer container 1 through the air pipe 70 when in operation, so that positive pressure or negative pressure in the transfer container 1 is realized, the second air filter 71 is used for filtering impurities in air to prevent internal pollution of the culture appliance, and in addition, particles possibly occur when the peristaltic pump 13 clamps the air pipe 70 when in operation, the second air filter 71 can be arranged between the peristaltic pump 13 and the ventilation connecting part 11.

In this embodiment, the transfer container 1 is a rigid container. In this way, the relay container 1 is not deformed in the positive and negative pressure state.

In this embodiment, the pipe 10 is provided with a sixth interface 101 for leak detection. The sixth abutting portion 101 can detect whether the pipe 10 is leaked or not and the sealing condition of the pipe 10, and the test is generally completed by the sixth abutting portion 101 before the disposable culture instrument is assembled and shipped.

In this embodiment, the culture vessel 2 has at least two inner sides of different areas, and the inner sides of different areas form respective culture surfaces for culturing different volumes of cell solutions.

Along with the increase of cell solution volume, through rotating culture vessel 2, bear cell solution to the cultivation face of corresponding area on cultivate, make the degree of depth of cell solution be unlikely to too low, prevent the evaporation of cell solution very fast, also be unlikely to too high, prevent that cell density is too low, the expansion speed is slow and the cell in the middle of the cell solution can't contact with the air, satisfy the cell solution's of volume continuous increase cultivate demand, improved the cell culture effect.

The disposable cell culture apparatus of this embodiment can be assembled manually or by machine in a clean workshop, then packaged with a sealed packaging bag, sterilized and shipped after packaging, and when in use, a user only needs to butt-joint a container filled with a solution in a sterile environment or inject the solution into an existing container, and then install the container on the installation panel 9.

In this embodiment, as shown in FIGS. 6 and 7, the disposable cell culture apparatus further comprises a packing bag 12, and each container and the tube 10 are packed in the packing bag 12.

In this embodiment, the pipe 10 and the container may be connected by a Luer (Luer) or a two-way connector, that is, the connection portion is a Luer (Luer) or a two-way connector, or the pipe 10 and the container may be connected by a connection device, for example, a hot-melt welding machine.

Embodiment two:

FIG. 8 shows an embodiment of the cell culture apparatus of the present utility model, which comprises a mounting panel 9 and the disposable cell culture instrument of the first embodiment, wherein a plurality of mounting portions 91, a plurality of pinch valves 92 and a plurality of fixing pipes 93 are provided on the mounting panel 9, the relay container 1 is detachably mounted on the mounting portion 91, the pipeline 10 is fixed on the fixing pipes 93, and the pinch valves 92 are clamped on the corresponding pipe positions. The disposable cell culture apparatus is detachably arranged on the mounting panel 9, and is scrapped after being used. The culture vessel 2, the initial vessel 3, the culture medium vessel 4, and the like are provided in the culture apparatus or on a hanger. Thus, pinch valve 92 is not disposable with a disposable cell culture apparatus, can be used multiple times, and saves cost.

While the utility model has been described in terms of preferred embodiments, it is not intended to be limiting. Many possible variations and modifications of the disclosed technology can be made by anyone skilled in the art, or equivalent embodiments with equivalent variations can be made, without departing from the scope of the utility model. Therefore, any simple modification, equivalent variation and modification of the above embodiments according to the technical substance of the present utility model shall fall within the scope of the technical solution of the present utility model.

Claims (20)

1. A disposable cell culture apparatus, characterized in that: the culture medium comprises a transfer container (1), a culture container (2) and a pipeline (10) for communicating the transfer container (1) and the culture container (2), wherein the transfer container (1) is provided with a ventilation connecting part (11) for enabling the inside of the transfer container to be ventilated to generate positive pressure or negative pressure, and the pipeline (10) is provided with a first butt joint part (31) for connecting an initial container (3) and a second butt joint part (41) for connecting a culture medium container (4).

2. The disposable cell culture apparatus of claim 1 wherein: the first docking portion (31) is docked with an initial container (3) and/or the second docking portion (41) is docked with a culture medium container (4).

3. The disposable cell culture apparatus of claim 1 wherein: the pipeline (10) is provided with a third butt joint part (51) for connecting the collecting container (5).

4. A disposable cell culture apparatus according to claim 3, wherein: the third butt joint part (51) is butt-jointed with a collecting container (5).

5. The disposable cell culture apparatus of claim 4 wherein: the collection container (5) is provided with a sampling interface (52) and/or a first gas filter (53).

6. The disposable cell culture apparatus of claim 4 wherein: the pipeline (10) for communicating the collecting container (5) with the initial container (3) is provided with a demagnetizing bead assembly (54).

7. The disposable cell culture apparatus of claim 1 wherein: the pipeline (10) is provided with a fourth butt joint part (61) for connecting the sampling container (6).

8. The disposable cell culture apparatus of claim 7 wherein: the fourth butt joint part (61) is butt-jointed with a sampling container (6).

9. The disposable cell culture apparatus of claim 1 wherein: a counter (80) for counting is arranged between the culture container (2) and the transfer container (1).

10. The disposable cell culture apparatus of claim 1 wherein: the pipeline (10) is provided with a fifth butt joint part for connecting a waste liquid container.

11. The disposable cell culture apparatus of claim 10 wherein: the fifth butt joint part is butt-jointed with a waste liquid container.

12. The disposable cell culture apparatus of claim 10 wherein: a waste liquid pipeline (20) for discharging waste liquid is arranged between the culture container (2) and the transfer container (1).

13. The disposable cell culture apparatus of claim 1 wherein: the pipeline (10) is connected with the bottom of the transfer container (1).

14. The disposable cell culture apparatus of claim 13 wherein: the pipeline (10) is also connected with the top of the transfer container (1).

15. The disposable cell culture apparatus of any one of claims 1 to 14 wherein: the ventilation connecting part (11) is arranged on the side wall or the top wall of the transfer container (1).

16. The disposable cell culture apparatus of any one of claims 1 to 14 wherein: the ventilation connecting part (11) is connected with a positive and negative pressure device (7), the positive and negative pressure device (7) comprises a second gas filter (71) and a gas pipe (70), and the second gas filter (71) is communicated with the transfer container (1) through the gas pipe (70).

17. The disposable cell culture apparatus of any one of claims 1 to 14 wherein: the transfer container (1) is a hard container.

18. The disposable cell culture apparatus of any one of claims 1 to 14 wherein: the pipeline (10) is provided with a sixth butt joint part (101) for leakage detection.

19. The disposable cell culture apparatus of any one of claims 1 to 14 wherein: the culture vessel (2) has at least two inner sides of different areas, which form respective culture surfaces for culturing different volumes of cell solution.

20. A cell culture apparatus, characterized in that: the disposable cell culture apparatus comprises a mounting panel (9) and any one of claims 1 to 19, wherein a plurality of mounting parts (91), a plurality of pinch valves (92) and a plurality of fixed pipe fittings (93) are arranged on the mounting panel (9), the transit container (1) is detachably arranged on the mounting parts (91), the pipeline (10) is fixed on the fixed pipe fittings (93), and the pinch valves (92) are clamped on corresponding pipe positions.