CN219689718U - Microorganism interpretation instrument - Google Patents

Abstract

The utility model belongs to the field of microorganism detection devices, and relates to a microorganism interpretation instrument which can be suitable for microorganism interpretation of a quantitative tray and a plate and has wide application. The microorganism interpretation instrument comprises a box body, wherein a light path cavity is formed in the box body to form at least two light paths, so that interpretation of at least two quantitative trays and plates is met, functions of the microorganism interpretation instrument are diversified, and the application range is expanded.

Description

Microorganism interpretation instrument

Technical Field

The utility model belongs to the field of microorganism detection devices, and relates to a microorganism interpretation instrument which can be suitable for microorganism interpretation of a quantitative tray and a plate and has wide application.

Background

The total number of bacterial colonies is an overall indicator of the microbial contamination level, and the current method for detecting the total number of bacterial colonies is mainly plate count method, namely, the total number of bacterial colonies which can grow on a common nutrient agar plate after being cultured for 48 hours at 37 ℃ under the aerobic condition. The plate count method, which was used for 30 years before 1985, included the enzyme substrate method (simple method) to detect the total number of colonies in water in EPA9215E, and the method mainly uses simple discs for sample culture and enzymatic reaction. The coliform is a kind of coliform which is mostly existed in the excrement of warm-blooded animals, the places where human beings frequently move and the places where the excrement is polluted, and the pollution of human and animal excrement to the external environment is the main cause of the coliform in the nature. Coliform is one of the important indicators for evaluating the hygienic qualities of drinking water and food. GB/T5750-2006 Standard test method for Drinking Water, enzyme substrate method is added to detect coliform group. The enzyme substrate method is also called quantitative Tray method (Quanti-Tray) which is a novel method for measuring coliform in water, the principle is based on the principle that bacterial specific enzyme reacts with substrate to generate yellow fluorescence reaction, after 24 hours of culture, the final colony count is obtained through statistical analysis, and the colony count of 2419MPN/mL can be detected under the condition of no dilution.

The apparatus for identifying and reading the number of microorganisms involved in the plate count method, the simple method and the quantitative plate method is called a "microorganism reader", and the conventional microorganism reader cannot be used for simultaneously identifying a plurality of quantitative plates and plates involved in the plate count method, the simple method and the quantitative plate method, and has limited functions and application range.

Disclosure of Invention

The inventor creatively performs reasonable design and layout on the light source in the microbial interpretation instrument, forms a light path cavity in the box body of the microbial interpretation instrument, and forms at least two light paths so as to meet the interpretation of at least two quantitative trays and plates, so that the microbial interpretation instrument has diversified functions and expanded application range. Based on the above, the embodiment of the utility model at least discloses the following technical scheme:

(1) The embodiment of the utility model discloses a multipurpose microorganism interpretation instrument, which comprises the following components:

the light transmission device comprises a shell, wherein a light transmission chamber is formed in the shell, and comprises a bright chamber and a dark chamber;

a light source configured to transmit a composite light in the darkroom and/or transmit a fluorescent light in the darkroom;

the carrier assembly is movably arranged in the light transmission chamber and is provided with a plurality of carrying parts and a plurality of light transmission parts, the carrying parts are used for carrying a quantitative tray or a plate for detecting microorganisms and carrying the compound light transmitted in the bright chamber or the fluorescence transmitted in the dark chamber, and the light transmission parts are used for transmitting the compound light and/or the fluorescence reflected and/or transmitted by the quantitative tray or the plate; and

and the photographing component is provided with an image pickup end extending into the light transmission chamber, and the image pickup end is used for picking up composite light or fluorescence transmitted and/or reflected by the quantitative tray or the plate at the bearing part.

(2) The embodiment of the utility model discloses a microorganism interpretation instrument suitable for a quantitative tray and a plate, which comprises the following steps:

the box body is internally provided with a light path cavity, and the light path cavity is provided with a first end and a second end;

the light source is configured in the light path cavity, and light emitted by the light source forms at least two different light paths between the first end and the second end;

a tray assembly for carrying a quantitative tray or plate for detecting microorganisms, the tray assembly being movably disposed between the first end and the second end, the tray assembly having a light-transmitting portion in the light path; and

and the photographing component is configured at the second end and is used for photographing the composite light and/or fluorescence transmitted and/or emitted by the quantitative tray or the plate.

The multipurpose microorganism interpretation instrument or the microorganism interpretation instrument applicable to the quantitative tray and the plate provided by the embodiment of the utility model can simultaneously meet the interpretation of the quantitative tray and the plate, such as the interpretation of a 97-hole quantitative tray, a 51-hole quantitative tray or a 96-hole quantitative tray, a simple disc, an EesyDisc plate or a common plate, has strong practicability, can save a large amount of cost for the microorganism interpretation process, has accurate interpretation results and is not influenced.

Drawings

Fig. 1 is a schematic plan view of a multipurpose microorganism reader according to an embodiment of the present utility model.

Fig. 2 is a schematic plan view of a multipurpose microorganism reader according to an embodiment of the present utility model.

Fig. 3 is a schematic plan view of a multipurpose microorganism reader according to an embodiment of the present utility model.

Fig. 4 is a schematic plan view of a multipurpose microorganism reader according to an embodiment of the present utility model.

Fig. 5 is a schematic plan view of a multipurpose microorganism reader according to an embodiment of the present utility model.

Fig. 6 is a schematic plan view of a carrier assembly according to an embodiment of the present utility model.

Fig. 7 is a schematic perspective view of an adapter according to an embodiment of the present utility model.

FIG. 8 is a schematic diagram showing a perspective structure of a microbiological assay for quantifying trays and plates according to an embodiment of the present utility model; comprises an integral assembly drawing and a perspective drawing after the box body is disassembled.

FIG. 9 is a schematic diagram showing a perspective structure of a microbiological assay for quantifying trays and plates according to an embodiment of the present utility model; comprises a tray component, a light source, a photographing component and a part of box body structure diagram.

Fig. 10 is a schematic diagram showing a perspective structure of a microorganism assay suitable for use in a quantitative tray and a plate according to an embodiment of the present utility model.

Fig. 11 is a schematic perspective view of a tray frame according to an embodiment of the present utility model.

Fig. 12 is a schematic diagram of a three-dimensional structure of an aptamer according to an embodiment of the utility model.

Fig. 13 is a schematic plan view of a multipurpose microorganism reader according to an embodiment of the present utility model.

Fig. 14 is a schematic perspective view of a tray frame according to an embodiment of the present utility model.

Fig. 15 is a schematic perspective view of a tray frame according to an embodiment of the present utility model.

Fig. 16 is a schematic perspective view of a tray frame according to an embodiment of the present utility model.

Fig. 17 is a schematic diagram of a three-dimensional structure of an aptamer according to an embodiment of the utility model.

Reference numerals: the light source device comprises a housing 100, a light transmission chamber 101, a light chamber 102, a darkroom 103, a light reflection layer 104, a light absorption layer 105, a first housing 100a, a second housing 100b, a flexible member 100c, two rollers 100d, a light source 200, a first light source 201, a second light source 202, a carrier assembly 300, a carrying part 301, a first placing groove 3011, a second placing groove 3012, a light transmitting part 302, a circular-like part 3021, a middle part 3022, an adapter 303, a first inclined surface 3030, a first arc-shaped edge 3031, a photographing assembly 400, and an image capturing end 401.

The light path module comprises a box 700, a light path cavity 701, a first end 702, a second end 703, a light reflecting layer 704, a light absorbing layer 705, a tray assembly 800, a light transmitting hole 801, a round-like hole 8010, a middle hole 8011, a tray frame 802, a limit hole 8020, an adapter 803, a second inclined plane 8030, a second arc edge 8031, a limit post 8032, a bearing groove 804, a first bearing groove 8040, a second bearing groove 8041, a third bearing groove 8042, a light path 900, a first light path 901 and a second light path 902.

Detailed Description

In order to make the objects, technical solutions and advantages of the present utility model more apparent, the present utility model will be described in further detail with reference to the following examples. It should be understood that the specific embodiments described herein are for purposes of illustration only and are not intended to limit the scope of the utility model.

Various exemplary embodiments will now be described more fully with reference to the accompanying drawings, in which some exemplary embodiments are shown. In the figures, the thickness of lines, layers and/or regions may be exaggerated for clarity.

Thus, while the exemplary embodiments are capable of various modifications and alternative forms, embodiments thereof are shown by way of example in the drawings and will herein be described in detail. It should be understood, however, by those skilled in the art, that the exemplary embodiments are not limited to the particular forms disclosed, but on the contrary, the exemplary embodiments are to cover all modifications, equivalents, and alternatives falling within the scope of the utility model. Throughout the description of the drawings, the same reference numerals refer to the same or like elements.

It will be understood that when an element is referred to as being "connected" to another element, it can be directly connected or coupled to the other element or intervening elements may be present. In contrast, when an element is referred to as being "directly connected" to another element, there are no intervening elements present. Other expressions used to describe the relationship between elements may be interpreted in a similar manner (e.g., "between …" and "directly between …", "adjacent" and "directly adjacent", etc.).

The terminology used herein is for the purpose of describing particular embodiments only and is not intended to be limiting of example embodiments. As used herein, the singular forms "a", "an" and "the" are intended to include the plural forms as well, unless the context clearly indicates otherwise. It will be further understood that the terms "comprises," "comprising," "includes," and/or "including," when used herein, specify the presence of stated features, integers, steps, operations, elements, and/or components, but do not preclude the presence or addition of one or more other features, integers, steps, operations, elements, components, and/or groups thereof.

Unless otherwise defined, all terms (including technical and scientific terms) used herein have the same meaning as commonly understood by one of ordinary skill in the art to which example embodiments belong. It will be further understood that terms, such as those defined in commonly used dictionaries, should be interpreted as having a meaning that is consistent with their meaning in the context of the relevant art and will not be interpreted in an idealized or overly formal sense unless expressly so defined herein.

It will be appreciated by those skilled in the art that the term "and/or" as used herein is merely one association relationship describing associated objects, meaning that there may be three relationships, e.g., a and/or B, may represent: a exists alone, A and B exist together, and B exists alone. In addition, the character "/" herein generally indicates that the front and rear associated objects are an "or" relationship.

It should be noted that, the terms "upper", "lower", "left", "right", and the like in the embodiments of the present utility model are described in terms of the angles shown in the drawings, and should not be construed as limiting the embodiments of the present utility model. In the context of this document, it will also be understood that when an element is referred to as being "on" or "under" another element, it can be directly on the other element or be indirectly on the other element or under via intervening elements. In describing embodiments of the present utility model, it should be understood that the terms "first," "second," "third," "fourth," "fifth," and the like are used merely to distinguish between descriptions and should not be construed as indicating or implying relative importance.

As shown in fig. 1, an embodiment of the present utility model discloses a multipurpose microorganism reading apparatus, which comprises a housing 100, a light source 200, a carrying assembly 300 and a photographing assembly 400. The housing 100 has a light transmission chamber 101 formed therein, and the light transmission chamber 101 includes a bright chamber 102 and a dark chamber 103. The light source 200 is configured to emit a composite light into the light chamber 102 and/or to emit fluorescent light into the dark chamber 103. The carrier assembly 300 is movably disposed in the light transmission chamber 101, the carrier assembly 300 has a plurality of carrying parts 301 and a plurality of light transmission parts 302, the carrying parts 301 are used for carrying a quantitative tray or a plate for detecting microorganisms and carrying the compound light transmitted in the light chamber 102 or the fluorescent light transmitted in the dark chamber 103, and the light transmission parts 302 are used for transmitting the compound light and/or the fluorescent light reflected and/or transmitted by the quantitative tray or the plate. The photographing assembly 400 has an image capturing end 401 extending into the light transmission chamber 101, the image capturing end 401 for capturing the composite light or fluorescence transmitted and/or reflected by the dosing disc or plate at the carrier 301.

It should be understood by those skilled in the art that "configured" herein should be understood that the component is mounted, connected or placed at a certain location or connected with other components, and the mounting or connection may be a static connection or a dynamic connection, where the static connection includes a fixed connection, an indirect fixed connection through other intermediate components, and the dynamic connection includes a sliding connection, a rotating connection, etc., and the specific connection manner is not limited. In some embodiments, "configured" includes a fixed connection, such as by a connection (e.g., a screw, etc.), or a weld. In some embodiments, "movably disposed" includes a sliding connection, a rolling connection, or a rotating connection, such as a sliding connection by a rail, for example, the rail sliding connection may be a manual driving sliding movement or an electric driving sliding movement, and the sliding connection manner is not particularly limited.

The multipurpose microorganism interpretation instrument provided by the embodiment of the utility model can form a bright room or a dark room in the microorganism interpretation instrument or form the bright room and the dark room at the same time, has stronger adaptability to a quantitative tray or a plate which needs compound light and/or fluorescence for microorganism interpretation, enriches the application of the microorganism interpretation instrument, realizes standardization and digitization of microorganism detection in different types of quantitative trays or plates, has the advantages of simple and rapid operation and expandable function, and can effectively improve the working efficiency of detection personnel and the accuracy of detection results.

In some embodiments, the light source 200 comprises a first light source 201 for emitting a composite light and a second light source 202 for emitting fluorescence light; at least one first light source 201 is disposed in the light chamber 102, and at least one second light source 202 is disposed in the dark chamber 103. As will be appreciated by those skilled in the art, the composite light emitted by the first light source 201 can create a bright field light environment within the bright room 102 to meet the detection and interpretation requirements of a quantification disk or plate carried on the carrier 301. Likewise, the composite light from the second light source 202 can create a dark field light environment within the darkroom 103 to meet the detection and interpretation requirements of the quantitation plate or dish carried on the carrier 301.

In some embodiments, as shown in fig. 1-3, the light chamber 102 is contiguous with the dark chamber 103. The housing 100 includes a light reflecting layer 104 formed in the light chamber 102 and a light absorbing layer 105 formed in the dark chamber 103, the light reflecting layer 104 being located around the first light source 201, the light absorbing layer 105 being located around the second light source 202. Those skilled in the art will appreciate that the term "meet" herein refers to the edges of two parts (e.g., light chamber 102 and dark chamber 103) that coincide, are in contact, are in abutment, or are in close proximity to each other. For example, the light reflecting layer 104 encloses the light chamber 102, the light absorbing layer encloses the dark chamber 103, a separation line is formed at the junction of the light chamber 102 and the dark chamber 103, the first light source 201 extends to the center of the light chamber 102, and the second light source 202 extends to the center of the dark chamber 103. When a bright field light environment needs to be formed, the first light source 201 is turned on, the first light source 201 emits composite light, the composite light is reflected by the direct light and the light reflection layer 104 to form bright field light, the bright field light irradiates the quantitative tray or the plate on the bearing part 301, and the light reflected or transmitted by the quantitative tray or the plate is taken by the image taking end 410, so that the interpretation of microorganisms in the quantitative tray or the plate is realized. Similarly, when a dark field light environment needs to be formed, the second light source 202 is turned on, the second light source 202 emits fluorescence, and the fluorescence is absorbed and reflected by the direct light and light absorbing layer 105 to form dark field fluorescence, which irradiates onto the quantifying tray or the plate on the carrying portion 301, and the light reflected or transmitted by the quantifying tray or the plate is taken by the image taking end 410, so that the important microorganism interpretation of the quantifying tray or the plate is realized.

In some embodiments, as shown in fig. 4-5, the housing 100 includes a switchable light reflecting layer 104 and a light absorbing layer 105; the light reflection layer 104 is used for forming the bright room 104, and the light absorption layer 105 is used for forming the dark room 103; the first light source 201 can transmit the composite light in the light chamber 102, and the second light source 202 can transmit the fluorescent light in the dark chamber 103. It will be understood by those skilled in the art that the term "inter-switching" herein refers to the fact that two parts (e.g. the light reflecting layer 104 and the light absorbing layer 105) need only be used one within the light transmitting chamber 101 and the other can be hidden when detection of a dosing disc or plate is required, while the other can be configured into the light transmitting chamber 101 by some action when the other part is required, such that only one or both parts are required within the light transmitting chamber 101.

For example, as shown in fig. 4 to 5, the housing 100 includes a first housing 100a, a second housing 100b, a flexible member 100c, two rollers 100d, and two motors (not shown). The second housing 100b is sleeved in the first housing 100a, the second housing 100b is made of transparent material, and the second housing 100b encloses the light transmission chamber 101. The two motors are respectively connected with the two rollers 100d to drive the two rollers 100d to rotate. The flexible member 100c is annularly abutted in a sleeve joint gap between the second housing 100b and the first housing 100a, and the flexible member 100c has a light reflection layer 104 and a light absorption layer 105 arranged along a circumferential direction of the annular light transmission chamber 101. One end of the flexible member 100c is connected to one of the rollers 100d, and the other end is connected to the other roller 100d after being looped along a sleeve joint gap between the second housing 100b and the first housing 100 a. In this way, turning on one of the motors can rotate one of the rollers 100d, so that the flexible member 100c moves between the first housing 100a and the second housing 100b, and the light reflection layer 104 and the light absorption layer 105 of the flexible member 100c abutting on the second housing 100b are switched. In some embodiments, the flexible member 100c includes a base material sewn with cloth or fiber, a light reflecting layer 104 and a light absorbing layer 105 attached to one side thereof abutting against the second housing 100 b.

For example, the light reflecting layer 104 may be various kinds of tinfoil (e.g., orange peel tinfoil), various types of reflective films (e.g., lens embedded reflective film, lens sealed reflective film, prism reflective film, etc.), various types of retroreflective materials (e.g., glass bead type retroreflective material-retroreflective cloth, retroreflective lattice, etc.), but is not limited to a particular material. Optionally, the light reflective layer 104 has a reflectance of between 20 and 70%, optionally 20%, 21%, 22%, 23%, 24%, 25%, 26%, 27%, 28%, 29%, 30%, 31%, 32%, 33%, 34%, 35%, 36%, 37%, 38%, 39%, 40%, 41%, 42%, 43%, 44%, 45%, 46%, 47%, 48%, 49%, 50%, 51%, 52%, 53%, 54%, 55%, 56%, 57%, 58%, 59%, 60%, 61%, 62%, 63%, 64%, 65%, 66%, 67%, 68%, 69%, 70%, or a value whose reflectance is substantially similar (substantially similar means within 1%).

For example, the light absorbing layer 105 may be various light absorbing cloths (e.g., black fleece), various light absorbing films (e.g., an Acktar Black light absorbing film in israel). Optionally, the light absorption layer 105 has a reflectance of between 1 and 5%, optionally 1%, 2%, 3%, 4%, 5%, or a value substantially similar to the reflectance (substantially similar means within 1%).

In some embodiments, the carrier assembly 300 is movably disposed within the light chamber 102. It should be understood by those skilled in the art that disposing the carrier assembly 300 in the light chamber 102 does not limit the light of the dark chamber 103 from being irradiated onto the weighing tray or plate placed on the carrying portion 301 through the light transmitting portion 302.

In some embodiments, the carrier assembly 300 is movably disposed within the darkroom 103. It should be understood by those skilled in the art that the carrier assembly 300 is disposed in the darkroom 103, and the light of the darkroom 102 is not limited to being irradiated onto the weighing tray or the plate placed on the carrying portion 301 through the light transmitting portion 302.

In some embodiments, the carrier assembly 300 is movably disposed at the junction of the light chamber 102 and the dark chamber 103. It will be appreciated by those skilled in the art that in these embodiments, either the light of the light chamber 102 or the light of the dark chamber 103 can be irradiated onto the dosing disc or plate placed on the carrying portion 301 via the light-transmitting portion 302.

In some embodiments, as shown in fig. 7, the carrier assembly 300 further includes a plurality of adapters 303, the adapters 303 cooperating with the carrier 301 such that the metering disc or plate is placed in the light chamber 102, or the dark chamber 103, or the junction of the light chamber 102 and the dark chamber 103. It will be appreciated by those skilled in the art that different sizes and shapes of metering discs or plates may be accommodated by portions of the edges of the translucent portion 302. However, to accommodate the contours and shapes of a wider variety of metering discs or plates, in some embodiments, as shown in FIG. 6, the light transmissive portion 302 is configured as two circular-like portions 3021 and an intermediate portion 3022 connecting the two circular-like portions. The adapter 303 not only enables different kinds of quantitative trays or plates to be fixedly placed on the bearing portion 301, but also enables the to-be-detected portion (for example, each small hole in the 97 quantitative tray) of the quantitative tray or plate to face the light-transmitting portion 302 so as to facilitate light passing. In some embodiments, two quasi-circular portions 3021 may be used to detect and judge two identical or different plates at the same time, while the adapter 303 is used to cover the middle portion 3022 to adjust and fix the position of the two planes 600 and to prevent light from being transmitted from the middle portion 3022.

In some embodiments, as shown in fig. 6 to 7, the carrier 301 is formed with a first placing groove 3011 adapted to a dosing disc, and the adapter 303 has a first inclined surface 3030, the first inclined surface 3030 being used to support the dosing disc placed in the first placing groove 3011. The culture solution for detecting the substrate containing the enzymatic reaction is contained in the quantitative tray, if a small amount of bubbles are mixed in the quantitative tray during filling, the color judgment of the quantitative tray is affected when the quantitative tray is horizontally placed for detection, one end of the quantitative tray is lifted by the first inclined plane 3030, the surface of the quantitative tray is at the same angle as the first inclined plane 3030, so that the bubbles in the center of the quantitative tray float to the highest point of each small hole and are positioned at the edge of the small hole, and the color of the small hole or the interpretation of microorganisms in the small hole by the bubbles is reduced or avoided.

In some embodiments, as shown in fig. 6-7, the carrier 301 is formed with a second slot 3012 for accommodating a plate, and the adapter 303 has a first arcuate edge 3031 for accommodating a plate arc, the first arcuate edge 3031 being for abutting against a plate arc edge placed in the second slot 3012. To accommodate various circular dishes of varying sizes and to enable the dish placed in the second slot 3012 to remain centered in the light-transmitting portion 302, the first arcuate edge 3031 can serve this purpose.

The embodiment of the utility model discloses a microorganism reader suitable for a quantitative tray and a plate, as shown in fig. 8 to 10, comprising a box 700, a light source 200, a tray assembly 800 and a photographing assembly 400. The housing 700 has an optical path cavity 701 formed therein, the optical path cavity 701 having a first end 702 and a second end 703. The light source 200 is disposed within the light path cavity 701, and light emitted by the light source 200 forms at least two different light paths 900 between the first end 702 and the second end 703. A tray assembly 800 for carrying a quantitative tray or plate for detecting microorganisms, the tray assembly 800 being movably disposed between the first end 702 and the second end 703, the tray assembly 800 having a light transmission hole 801 in an optical path; the photographing assembly 400 is configured at the second end 703 for photographing the composite light or fluorescence transmitted and/or emitted by the quantitative tray or plate.

The microbial interpretation instrument suitable for the quantitative tray and the plate provided by the embodiment of the utility model can meet the detection and interpretation requirements of the quantitative tray or the plate for microbial interpretation by compound light and/or fluorescence through forming the light path cavity with at least two different light paths in the microbial interpretation instrument, for example, the interpretation requirements of a 97-hole quantitative tray, a 51-hole quantitative tray or a 96-hole quantitative tray, a simple disc, an EesyDisc plate or a common plate are met, the practicability is strong, a large amount of cost can be saved for the microbial interpretation process, and the interpretation result is accurate and is not influenced.

In some embodiments, as shown in fig. 10, the optical path 900 includes a first optical path 901 (dashed line in the figure) and a second optical path 902 (dashed line in the figure) that are incident from two parallel surfaces of a quantitative tray or plate carried on the tray assembly 800, the first optical path 901 having a composite light formed therein, and the second optical path 902 having a fluorescent light formed therein.

In some embodiments, the inner wall of the case 700 is formed with a light reflecting layer 704 for reflecting the composite light and/or fluorescence emitted from the light source 200, and a light absorbing layer 705 for absorbing the composite light and/or fluorescence emitted from the light source 200; wherein the light reflecting layer 704 is connected with the light absorbing layer 705.

In some embodiments, as shown in fig. 8-12, the light source 200 includes a first light source 201 for emitting a composite light and a second light source 202 for emitting fluorescence. The first light source 201 has a plurality of first light sources, and is disposed at the first end 702, the second end 703, and the junction between the light reflecting layer 704 and the light absorbing layer 705. The second light source 202 is disposed at the junction of the light reflecting layer 704 and the light absorbing layer 705. The first light source 201 may be in the form of a long bar, a ring, or the like, and the shape thereof is not particularly limited.

In some embodiments, the first light source 201 may be a tungsten halogen lamp, a metal halide lamp, a high pressure sodium lamp, an LED light source, a xenon lamp, a mercury lamp, an electroluminescent light source, a light emitting diode, a carbon arc lamp, an incandescent lamp, or a low pressure sodium lamp, without limitation. In some embodiments, the second light source 202 is a fluorescent light source, such as blue fluorescence, violet fluorescence, or yellow fluorescence, and in one embodiment, the second light source 202 is a fluorescent lamp that emits a 365nm light source. For example, the second light source 202 is selected from a first/second/third/fourth generation ALPD fluorescent laser light source, an SSI fluorescent laser light source, an LED light source.

For example, when the first light source 201 is turned on, the first light source 201 emits a composite light, the composite light is reflected by the direct light and the reflective layer 704 to form bright field light, and the bright field light irradiates the quantifying tray or the plate on the tray assembly 800, and the light reflected or transmitted by the quantifying tray or the plate is acquired by the photographing assembly 800, so that the interpretation of the microorganisms in the quantifying tray or the plate is realized. Similarly, when the second light source 202 is turned on, the second light source 202 emits fluorescence, and bright field light is formed after reflection by the direct light and the light absorption layer 705 and irradiates the quantitative tray or the plate on the tray assembly 800, and the light reflected or transmitted by the quantitative tray or the plate is acquired by the photographing assembly 800, so that the interpretation of microorganisms in the quantitative tray or the plate is realized.

For example, the light reflecting layer 704 may be various kinds of tinfoil (e.g., orange peel tinfoil), various types of light reflecting films (e.g., lens embedded type light reflecting film, lens sealed type light reflecting film, prism type light reflecting film, etc.), various types of light reflecting materials (e.g., glass bead type light reflecting material-light reflecting cloth, light reflecting lattice, etc.), but is not limited to a particular material. Optionally, the light reflective layer 104 has a reflectance of between 20 and 70%, optionally 21%, 22%, 23%, 24%, 25%, 26%, 27%, 28%, 29%, 30%, 31%, 32%, 33%, 34%, 35%, 36%, 37%, 38%, 39%, 40%, 41%, 42%, 43%, 44%, 45%, 46%, 47%, 48%, 49%, 50%, 51%, 52%, 53%, 54%, 55%, 56%, 57%, 58%, 59%, 60%, 61%, 62%, 63%, 64%, 65%, 66%, 67%, 68%, 69%, or a value where the reflectance is substantially similar (substantially similar means within 1%).

For example, the light-absorbing layer 705 may be various kinds of light-absorbing cloths (e.g., black flannel), various kinds of light-absorbing films (e.g., an aktar Black light-absorbing film in israel). Optionally, the light absorption layer 705 has a reflectance of between 1 and 5%, optionally 2%, 3%, 4%, or a value where the reflectance is substantially similar (approximately similar means within 1%).

In one embodiment, as shown in FIG. 13, a light reflecting layer 704 is formed in the optical path cavity 701 on a side near the second end 703, and a light absorbing layer 705 is formed in the optical path cavity 701 on a side near the first end 702. The tray assembly 800 is movably disposed at the light reflecting layer 704 and the light absorbing layer 705, and the first light source 201 at the first end 702 generates a composite light (e.g. an incandescent lamp emits a composite light) to a dosing tray or a flat panel on the tray assembly 800, and a part of the composite light is directly transmitted through the dosing tray or the flat panel, and is reflected by the light reflecting layer 704 and then is directed to the dosing tray or the flat panel, so as to form a first light path 901.

In one embodiment, as shown in fig. 13, the first light source 201 at the second end 703 generates a combined light (e.g., a combined light from an incandescent lamp) to a metering disc or dish on the tray assembly 800, and a portion of the combined light is directed through the metering disc or dish, reflected by the reflective layer 704, and directed toward the metering disc or dish to form the first light path 901.

In one embodiment, as shown in FIG. 13, the second light source 202, where the light reflecting layer 704 meets the light absorbing layer 705, generates a compound light (e.g., ultraviolet light fluoresces) to the dosing plate or plate on the tray assembly 800, a portion of which is directed through the dosing plate or plate to form a second light path 902.

In one embodiment, as shown in fig. 13, a tray assembly 800 is movably disposed at the second end 703. The first light source 201 at the first end 702 emits a combined light thereto forming a first light path 901 or the first light source 202 below the tray assembly 800 at the second end 703 emits a combined light to the tray assembly 800 forming a first light path 901. The second light source 202, at the junction of the light reflecting layer 704 and the light absorbing layer 705, emits fluorescence to the tray assembly 800 to form a second light path 902.

In some embodiments, as shown in fig. 14-17, the tray assembly 800 includes a tray rack 802 and at least one aptamer 803. The tray frame 802 is provided with at least one light hole 801 in the center and at least one bearing groove 804 in the periphery, the light hole 801 participates in forming a first light path 901 and a second light path 902, and the bearing groove 804 bears a quantitative tray or plate for detecting microorganisms.

In some embodiments, as shown in fig. 14-16, the tray 802 forms at least one limiting hole 8020 on the periphery of the carrying groove 804. As shown in fig. 17, the adapter 803 has at least one retention post 8032 for insertion into the retention aperture 8020 and for restraining or securing a metering disc or plate in the carrier slot 804.

In some embodiments, as shown in fig. 14 to 16, the tray frame 802 has a plurality of kinds of carrying grooves 804, the plurality of kinds of carrying grooves 804 are sequentially stacked in the thickness direction of the tray frame 802, and the projected area of the plurality of kinds of carrying grooves 804 in the thickness direction of the tray frame 802 is sequentially reduced.

In some embodiments, as shown in fig. 14-16, the tray rack 802 has a first carrying trough 8040 for carrying and holding a 97-well, 51-well, or 96-well quantitation plate of microorganisms. As shown in fig. 17, the at least one aptamer 803 has a second inclined surface 8030, and the second inclined surface 8030 is used for supporting a 97-hole dosing disc, a 51-hole dosing disc or a 96-hole dosing disc placed in the first carrying groove 8040. For example, the 97-hole, 51-hole or 96-hole quantitative tray is in a substantially flat plate shape, the first carrying groove 8040 is also in a substantially flat plate space, the adapter 803 can be placed at one end of the first carrying groove 8040, then the quantitative tray is placed, and the trapezoidal second inclined surface 8030 is used for lifting one end of the quantitative tray, so that bubbles are less lifted to the small hole edge of the quantitative tray, and the influence on judgment is reduced. In some embodiments, the aptamer 803 only needs to have a second inclined plane 8030, and the three-dimensional shape of the aptamer 803 is not limited, and one side of the aptamer 803 is projected to be a trapezoid, which is integrally long.

In some embodiments, as shown in fig. 14-16, the tray rack 802 has a second bearing slot 8041 for bearing and securing the simple puck. As shown in fig. 17, the at least one aptamer 803 has a second arcuate edge 8031 that conforms to a simple circular arc, the second arcuate edge 8031 for abutting a simple circular arc edge placed within a second carrier groove 8041. In some embodiments, the adaptor 803 may have a second arcuate edge 8031, without limitation to its specific shape.

In some embodiments, as shown in fig. 14-16, the tray rack 802 has a third carrying slot 8042 for carrying and holding eiesyidisc plates and plain plates. At least one adapter 803 is used to snap onto the light aperture 801 to adapt the eesydish and the regular dish to the light aperture 801. In some embodiments, the adaptor 803 is suitable for EesyDisc plate and common plate placed in the light hole 801, and its specific shape is not limited.

In some embodiments, as shown in fig. 14-16, to accommodate the detection and judgment of 97-well, 51-well, 96-well, simple, eesydisk, and plain plates simultaneously, the tray rack 802 is generally flat-plate shaped. The first bearing groove 8040 has a flat plate shape as a whole. The second bearing groove 8041 and the third bearing groove 8042 are both circular. The second bearing groove 8041 has a planar projected area larger than that of the third bearing groove 8042. In some embodiments, the second bearing channel 8041 and the third bearing channel 8042 can be at the same thickness position of the tray frame 802 or at different thickness positions.

In some embodiments, as shown in fig. 14-16, the tray frame 802 is provided with at least two light holes 801, each light hole 801 includes two circular-like holes 8010 and a middle hole 8011 connecting the two circular-like holes 8010, the two circular-like holes 8010 can detect and judge two identical or different dishes at the same time, and the adapter 803 is used to cover the middle hole 8011 to adjust and fix the positions of the two planes 600, and is used to prevent light from being transmitted from the middle hole 8011. As shown in fig. 9 to 12, the light transmission holes 801 may be two or three, one including the circular-like holes 8010 and the middle holes 8011, and one including only the circular-like holes 8011.

In some embodiments, the circular-like hole 8010 is used for detecting and interpreting the simple disk, and the second carrying groove 8041 and the circular-like hole 8010 are located at the same thickness position of the tray frame 802.

In some embodiments, the middle hole 8011 is used for detection and interpretation of eesydisk and regular plate, and the third bearing groove 8042 is located at the same thickness position of the tray frame 802 as the middle hole 8011.

In one application example, a 97-hole dosing disc is placed on the first carrying groove 8040 (as shown in fig. 14), and an adapter 803 with a bevel is placed in the first carrying groove to lift one end of the 97-hole dosing disc, and then the tray assembly 800 is moved to the optical path cavity 701; turning on the first light source 201 at the second end 703, and turning off the first light source 201 after reading the yellow image appearing in the 97-hole quantification disk; and then turning on the second light source 202 at the joint of the reflecting layer 704 and the light absorbing layer 705 to read the blue fluorescent image presented in the 97-hole quantitative disc, thus completing the image shooting of the 97-hole quantitative disc. It will be appreciated by those skilled in the art that image recognition and interpretation is performed by a conventional photographing assembly 400 (biometric camera, image analysis device, such as a CCD camera) internal to the microbial interpretation instrument.

In one application example, 4 simple disks are placed on 4 circular-like holes 8010, and simultaneously an adapter 803 as shown in fig. 17 is placed to cover the middle hole 8011, and then the tray assembly 800 is moved into the optical path cavity 701; and turning on the second light source 202 at the joint of the reflecting layer 704 and the light absorbing layer 705 to read the blue fluorescent image presented in the 97-hole quantitative disc, thus finishing the image shooting of the 97-hole quantitative disc.

In one application example, as shown in fig. 12, the aptamer 803 is placed on 4 circular-like holes 8010, and an EesyDisc plate or a normal plate is placed on the aptamer 803; simultaneously, the adaptor 803 shown in fig. 17 is put in to cover the middle hole 8011, and then the tray assembly 800 is moved into the optical path cavity 701; the first light source 201 at the first end 702 is turned on to read the image presented in the eesydish or normal dish.

The present utility model is not limited to the above-mentioned embodiments, and any changes or substitutions that can be easily understood by those skilled in the art within the technical scope of the present utility model are intended to be included in the scope of the present utility model.

Claims (16)

1. A multipurpose microbial interpretation instrument, comprising:

the light transmission device comprises a shell, wherein a light transmission chamber is formed in the shell, and comprises a bright chamber and a dark chamber;

a light source configured to emit a composite light into the light room and/or to emit a fluorescent light into the dark room;

the carrier assembly is movably arranged in the light transmission chamber and is provided with a plurality of carrying parts and a plurality of light transmission parts, the carrying parts are used for carrying a quantitative tray or a plate for detecting microorganisms and carrying the compound light transmitted in the bright chamber or the fluorescence transmitted in the dark chamber, and the light transmission parts are used for transmitting the compound light and/or the fluorescence reflected and/or transmitted by the quantitative tray or the plate; and

and the photographing component is provided with an image pickup end extending into the light transmission chamber, and the image pickup end is used for picking up composite light or fluorescence transmitted and/or reflected by the quantitative tray or the plate at the bearing part.

2. The multipurpose microbial identifier of claim 1, wherein the light source comprises a first light source for emitting the composite light and a second light source for emitting the fluorescent light;

at least one of the first light sources is disposed in the bright room, and at least one of the second light sources is disposed in the dark room.

3. The multi-purpose microorganism reader according to claim 2, wherein,

the bright room is connected with the dark room;

the shell comprises a light reflection layer formed in the bright room and a light absorption layer formed in the dark room, wherein the light reflection layer is positioned around the first light source, and the light absorption layer is positioned around the second light source.

4. The multi-purpose microorganism reader according to claim 2, wherein,

the shell comprises a light reflecting layer and a light absorbing layer which can be switched with each other;

the light reflection layer is used for forming the bright room, and the light absorption layer is used for forming the dark room;

the first light source can transmit composite light in the darkroom, and the second light source can transmit fluorescence in the darkroom.

5. The multipurpose microbial interpretation of claim 4, wherein the housing comprises:

a first housing;

the second shell is sleeved in the first shell and made of transparent materials, and the second shell is enclosed into the light transmission chamber;

a roller;

the motor is connected with the roller and drives the roller to rotate;

the end part of the flexible piece is connected with the rolling shaft, the middle part of the flexible piece is connected with the second shell and surrounds the sleeving gap of the first shell, and the middle part of the flexible piece is provided with the light reflection layer and the light absorption layer which are distributed along the peripheral direction of the light transmission chamber.

6. The multipurpose microbial identifier of claim 2, wherein the cargo assembly is movably disposed within the light chamber, or within the dark chamber, or at a junction of the light chamber and the dark chamber.

7. The multipurpose microbial identifier of claim 1, wherein the carrier assembly further comprises a plurality of adapters that cooperate with the carrier to place the plate or the metering disc in the light chamber, or in the dark chamber, or at the junction of the light chamber and the dark chamber.

8. The multipurpose microbial interpretation instrument of claim 7, wherein the carrier is formed with a first placement groove adapted to the dosing plate, the adapter having a bevel for supporting the dosing plate placed in the first placement groove.

9. The multipurpose microbial identifier of claim 7, wherein the carrier is formed with a second placement groove compatible with the plate, the adapter having an arcuate edge compatible with the plate arc for abutting against the plate arc edge placed in the second placement groove.

10. Microorganism reader suitable for use in quantitative trays and plates, comprising:

the box body is internally provided with a light path cavity, and the light path cavity is provided with a first end and a second end;

the light source is configured in the light path cavity, and light emitted by the light source forms at least two different light paths between the first end and the second end;

a tray assembly for carrying a quantitative tray or plate for detecting microorganisms, the tray assembly being movably disposed between the first and second ends, the tray assembly having a light-transmitting aperture in the light path; and

and the photographing component is configured at the second end and is used for photographing the composite light and/or fluorescence transmitted and/or emitted by the quantitative tray or the plate.

11. The microbial interpretation instrument of claim 10, the optical path comprising a first optical path into which the composite light is formed and a second optical path into which the fluorescent light is formed from two parallel surfaces of a quantitative tray or plate carried on the tray assembly.

12. The microbial interpretation apparatus of claim 10, the housing inner wall being formed with:

a light reflecting layer for reflecting the composite light and/or fluorescence emitted by the light source; and

a light absorbing layer for absorbing the composite light and/or fluorescence emitted by the light source;

wherein, the reflecting layer is connected with the light absorption layer.

13. The microbial interpretation apparatus of claim 12, the light source comprising:

the first light sources are used for emitting composite light and are respectively arranged at the first end, the second end and the joint of the reflecting layer and the light absorption layer;

and the second light source is used for emitting fluorescence and is configured at the joint of the reflecting layer and the light absorption layer.

14. The microbial interpretation instrument of claim 11, the tray assembly comprising:

the tray frame is provided with at least one light hole in the center and at least one bearing groove in the periphery, the light holes participate in forming the first light path and the second light path, the bearing groove bears a quantitative tray or a plate for detecting microorganisms, and the tray frame forms at least one limiting hole in the periphery of the bearing groove;

at least one aptamer having at least one limiting post for inserting into the limiting hole and restraining or fixing the quantitative tray or plate in the carrying groove.

15. The microorganism reading apparatus according to claim 14, wherein the tray frame has a plurality of kinds of carrying grooves, the plurality of kinds of carrying grooves are sequentially stacked in a thickness direction of the tray frame, and a projected area of the plurality of kinds of carrying grooves in the thickness direction of the tray frame is sequentially reduced.

16. The microbial interpretation instrument of claim 14, wherein the tray frame has a first carrying slot for carrying and holding a 97-well, 51-well or 96-well quantitative tray of microorganisms; at least one of the adaptors has a ramp for supporting a 97-hole, 51-hole or 96-hole dosing disc placed in the first bearing channel;

the tray frame is provided with a second bearing groove for bearing and fixing the simple disc; at least one of the adaptors has an arcuate edge conforming to a simple circular arc for abutting a simple circular arc edge disposed within the second load-bearing slot;

the tray frame is provided with a third bearing groove for bearing and fixing the EesyDisc plate and the common plate; at least one of the adaptors is adapted to snap-fit over the light-transmitting aperture such that the eesydish and the normal dish are adapted to the light-transmitting aperture.