CN219449689U - Cell filtration device - Google Patents
Cell filtration device Download PDFInfo
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- CN219449689U CN219449689U CN202320524023.7U CN202320524023U CN219449689U CN 219449689 U CN219449689 U CN 219449689U CN 202320524023 U CN202320524023 U CN 202320524023U CN 219449689 U CN219449689 U CN 219449689U
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- filter
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- sized filter
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
- Y02E60/00—Enabling technologies; Technologies with a potential or indirect contribution to GHG emissions mitigation
- Y02E60/10—Energy storage using batteries
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Abstract
The utility model discloses a cell filtration device, which comprises a filter shell, a filter head and a filter head, wherein the filter shell is provided with an installation cavity with an upper opening; the micron-sized filter is provided with a filter cavity with a lower opening, the micron-sized filter is sleeved in the installation cavity in a clearance way, and the lower opening of the micron-sized filter is sealed and buckled on the bottom wall of the installation cavity; a gland, which is sealed on the upper opening of the filter shell in an adapting way, and the lower surface of the gland is buckled on the top wall of the micron-sized filter; the connecting block is fixedly connected to the bottom wall of the mounting cavity, and an internal thread connecting hole is formed in the top wall of the connecting block; the fixed screw rod passes through the gland and the micron-sized filter in a sealing way, and the lower end of the fixed screw rod is in fit and screwed connection with the internal thread connecting hole; the liquid inlet pipe is arranged on the pressure cover, and the inner end of the liquid inlet pipe is communicated with the mounting cavity at the outer side of the micron-sized filter; the liquid discharge pipe is arranged on the bottom wall of the filter shell, and the inner end of the liquid discharge pipe is communicated with the filter cavity. The utility model has the advantages that the utility model can be easily connected to a harvesting liquid pipeline for in vitro cell culture, continuously filters impurities in the harvesting liquid and harvest cell suspension.
Description
Technical Field
The utility model relates to the technical field of in-vitro cell culture, in particular to a cell filtration device.
Background
In vitro cell culture is the most common technique in cell biology research and is an essential important link of cloning technology.
At present, in-vitro cell culture is most commonly carried out by adopting a centrifugal method for filtering and harvesting cells when the cells are harvested, the centrifugal method is to pour culture harvest liquid into a centrifugal machine, impurities and cell suspension are separated by utilizing rotation of the centrifugal machine, the impurities are heavy and can be submerged into the bottom of the centrifugal machine, the cell suspension is light and is positioned on the upper layer of the centrifugal machine, and then the cell suspension on the upper layer is poured out to carry out subsequent treatments such as concentration and clarification, so that the harvesting of the cells is completed.
The centrifugation method is suitable for small culture harvest liquid amount, and when the culture harvest liquid amount is large, the adverse factors such as poor centrifugation time, more replacement steps, easy bacterial contamination caused by exposure to air, aerosol generation and the like exist, so that the cell harvest efficiency is low, and the cell pollution is serious. Therefore, the existing method can directly adopt a centrifugal method or directly use a cell filter screen for a small amount of culture harvest liquid, but for large and medium-sized enterprises or scientific research institutions with large culture harvest liquid acquisition amount, the efficiency of in-vitro cell culture is greatly influenced by carrying out cell harvest step by step and in a small amount.
Disclosure of Invention
The utility model aims to provide a cell filtration device.
In order to achieve the above purpose, the present utility model may adopt the following technical scheme:
the cell filtration device of the present utility model comprises:
a filter housing having an upper open mounting cavity;
the micron-sized filter is provided with a filter cavity with a lower opening, the micron-sized filter is sleeved in the mounting cavity in a clearance manner, and the lower opening of the micron-sized filter is sealed and buckled on the bottom wall of the mounting cavity;
a gland, which is sealed on the upper opening of the filter shell in an adaptive way, and the lower surface of the gland is buckled on the top wall of the micron-sized filter;
the connecting block is fixedly connected to the bottom wall of the installation cavity at the inner side of the micron-sized filter, and an internal thread connecting hole is formed in the top wall of the connecting block;
the fixed screw rod sequentially passes through the gland and the micron-sized filter in a sealing way from top to bottom, and the lower end of the fixed screw rod is in fit and screwed connection with the internal thread connecting hole;
the liquid inlet pipe is arranged on the gland, and the inner end of the liquid inlet pipe is communicated with the mounting cavity at the outer side of the micron-sized filter; a kind of electronic device with high-pressure air-conditioning system
The liquid draining pipe is arranged on the bottom wall of the filter shell, and the inner end of the liquid draining pipe is communicated with the filter cavity.
The utility model has the advantages of simple structure, convenient use and low processing cost. Through setting up feed liquor pipe and the fluid-discharge tube of setting on filter housing diapire on pressing the lid, can easily connect on the result liquid pipeline of external cell culture, and then the clearance suit is in the micron-sized filter of filter housing's installation cavity, continues to filter the impurity in the result liquid, results cell suspension, the efficiency of result cell when improving external cell culture, satisfies the large and medium-sized enterprise of a large amount of result liquid or scientific research unit to the demand of cell results.
Preferably, the filter housing and the micron-sized filter are in cylindrical structures, and the filter housing, the micron-sized filter, the gland, the connecting block, the internal threaded connecting hole and the fixing screw are coaxially arranged so as to facilitate processing and subsequent assembly before use.
Preferably, a first annular groove is formed in the bottom wall of the installation cavity at the joint of the lower opening edge of the micron-sized filter and the lower opening edge of the micron-sized filter, and a first O-shaped sealing ring is arranged in the first annular groove to form effective sealing between the lower opening edge of the micron-sized filter and the bottom wall of the installation cavity of the filter shell, so that effective separation between the installation cavity outside the micron-sized filter and the filter cavity inside the micron-sized filter is ensured.
Preferably, a second annular groove is formed in the bottom wall of the gland at the joint of the top wall of the micron-sized filter, the center of the second annular groove is consistent with the center of the bottom wall of the gland, and a second O-shaped sealing ring is arranged in the second annular groove to form effective sealing at the joint of the gland and the micron-sized filter.
Preferably, a third annular groove is formed in the outer side wall of the gland along the circumferential direction, and a third O-shaped sealing ring is arranged in the third annular groove, so that effective sealing between the gland and the filter shell is formed, and the tightness of the installation cavity is ensured.
Preferably, the center of the top wall of the micron-sized filter is upwards protruded to form a clamping table, a clamping groove which is matched and clamped with the clamping table is formed in the center of the lower surface of the gland, and the gland and the micron-sized filter can be more accurate and convenient to assemble through the clamping of the clamping table and the clamping groove.
Preferably, a fourth annular groove is formed in the inner wall of the through hole in the center of the clamping table, which is attached to the fixing screw, along the circumferential direction, a fourth O-shaped sealing ring is arranged in the fourth annular groove, so that the clamping table of the micron-sized filter is effectively sealed at the attachment position of the clamping table and the fixing screw, and the sealing performance of the filter cavity is ensured.
Preferably, an end is coaxially arranged on the fixing screw rod positioned at the outer side of the gland, and a group of mutually symmetrical clamping planes are formed on one group of opposite side walls of the end, so that the fixing screw rod can be easily penetrated through the gland and the micron-sized filter by adopting a clamping spanner and is screwed and fixed on a connecting block on the bottom wall of the mounting cavity, and further fixation of the micron-sized filter can be easily realized.
Drawings
Fig. 1 is a schematic structural view of the present utility model.
Fig. 2 is an axial side view (exploded state) of fig. 1.
Detailed Description
The following description of the embodiments of the present utility model will be made clearly and fully with reference to the accompanying drawings, in which it is evident that the embodiments described are only some, but not all embodiments of the utility model. All other embodiments, which can be made by those skilled in the art based on the embodiments of the utility model without making any inventive effort, are intended to be within the scope of the utility model.
It should be noted that all directional indicators (such as up, down, left, right, front, and rear … …) in the embodiments of the present utility model are merely used to explain the relative positional relationship, movement, etc. between the components in a particular posture (as shown in the drawings), and if the particular posture is changed, the directional indicator is changed accordingly.
Furthermore, descriptions such as those referred to as "first," "second," and the like, are provided for descriptive purposes only and are not to be construed as indicating or implying a relative importance or implying an order of magnitude of the indicated technical features in the present disclosure. Thus, a feature defining "a first" or "a second" may explicitly or implicitly include at least one such feature.
As shown in fig. 1 and 2, the cell filtration device of the present utility model comprises a filter housing 1, a micron filter 2, a gland 3, a connecting block 4, a fixing screw 5, a liquid inlet pipe 6 and a liquid outlet pipe 7.
Wherein the filter housing 1 has an upper open mounting chamber 8; the micron-sized filter 2 is provided with a filter cavity 9 with a lower opening, and is sleeved in the installation cavity 8 of the filter shell 1 in a clearance manner, and the lower opening of the micron-sized filter 2 is sealed and buckled on the bottom wall of the installation cavity 8; the gland 3 is matched and sealed on the upper opening of the filter housing 1, and the lower surface of the gland 3 is buckled on the top wall of the micron-sized filter 2, so that the micron-sized filter 2 is tightly sealed in the mounting cavity 8 of the filter housing 1; the connecting block 4 is fixedly connected to the bottom wall of the installation cavity 8 at the inner side of the micron-sized filter 2, and is specifically integrally formed with the filter shell 1, and an internal thread connecting hole 10 is formed in the top wall of the connecting block 4; the fixed screw 5 sequentially passes through the gland 3 and the micron-sized filter 2 in a sealing way from top to bottom, and the lower end of the fixed screw 5 is in fit and screwed connection with the internal thread connecting hole 10, so that the gland 3 and the micron-sized filter 2 are further fixed; the liquid inlet pipe 6 is arranged on the gland 3, the outer end of the liquid inlet pipe is used for connecting the liquid outlet end of the culture harvest liquid of the in-vitro cell culture, and the inner end of the liquid inlet pipe is communicated with the mounting cavity 8 at the outer side of the micron-sized filter 2, so that the culture harvest liquid of the in-vitro cell culture is directly introduced into the filter shell 1; the liquid discharge pipe 7 is arranged on the bottom wall of the filter housing 1, the outer end of the liquid discharge pipe 7 is used as the final end of the harvested cell suspension, the connectable upper hose is butted in the collecting bottle, and the inner end of the liquid discharge pipe 7 is communicated with the filter cavity 9, so that culture harvest liquid filtered by the micron-sized filter 2 flows into the collecting bottle from the liquid discharge pipe 7, and the subsequent concentration, clarification and other treatments of the cell harvest liquid are facilitated. Through setting up feed liquor pipe 6 on gland 3 and setting up fluid-discharge tube 7 on filter housing 1 diapire, can easily connect on the result liquid pipeline of in vitro cell culture, and then the clearance suit is in the micron order filter 3 in the installation cavity 8 of filter housing 1, continues the impurity of filtering in the result liquid, results cell suspension, the efficiency of result cell when improving in vitro cell culture, satisfies the demand of large and medium-sized enterprise or scientific research unit of a large amount of result liquid to the cell harvest.
Specifically, the filter housing 1 and the micron-sized filter 2 may have cylindrical tubular structures, and the filter housing 1, the micron-sized filter 2, the gland 3, the connecting block 4, the internal threaded connection hole 10 and the fixing screw 5 are coaxially arranged, so as to facilitate processing and assembly. In addition, in order to improve the tightness of the whole cell filtration device, a first annular groove 11 is formed in the bottom wall of the installation cavity 8 at the joint part with the lower opening edge of the micron-sized filter 2, and a first O-shaped sealing ring 12 is arranged in the first annular groove 11; a second annular groove 13 is formed in the bottom wall of the gland 3 at the joint of the top wall of the micron-sized filter 2, the center of the second annular groove 13 is consistent with the center of the bottom wall of the gland 3, and a second O-shaped sealing ring 14 is arranged in the second annular groove 13; a third annular groove 15 is formed in the outer side wall of the gland 3 along the circumferential direction, and a third O-shaped sealing ring 16 is arranged in the third annular groove 15. Furthermore, in order to facilitate the rapid and accurate assembly of the gland 3 and the micron-sized filter 2, a clamping table 17 is formed by upwards protruding the center of the top wall of the micron-sized filter 2 during actual processing, correspondingly, a clamping groove 18 which is matched and clamped with the clamping table 17 is formed in the center of the lower surface of the gland 3, and the gland 3 and the micron-sized filter 2 can be more accurate and convenient during assembly through the matching and clamping of the clamping table 17 and the clamping groove 18; at this time, the fixing screw 5 should vertically pass through the center of the clamping table 17, and a fourth annular groove 19 is formed on the inner wall of the through hole at the center of the clamping table 17 attached to the fixing screw 5 along the circumferential direction, and a fourth O-ring 20 is disposed in the fourth annular groove 19.
Through the arrangement of the first annular groove 11 and the first O-shaped sealing ring 12, effective sealing between the lower opening edge of the micron-sized filter 2 and the bottom wall of the installation cavity 8 of the filter shell 1 is formed, and effective separation between the installation cavity 8 outside the micron-sized filter 2 and the filter cavity 9 inside the micron-sized filter 2 is ensured; through the arrangement of the second annular groove 13 and the second O-shaped sealing ring 14, effective sealing at the joint of the gland 3 and the micron-sized filter 2 is formed; through the arrangement of the third annular groove 15 and the third O-shaped sealing ring 16, effective sealing between the gland 3 and the filter shell 1 is formed, and the tightness of the installation cavity 8 is ensured; through the arrangement of the fourth annular groove 19 and the fourth O-shaped sealing ring 20, effective sealing is formed at the joint of the clamping table 17 of the micron-sized filter 2 and the fixed screw 5, and the tightness of the filter cavity 9 is ensured; and the tightness of the whole cell filtration device after assembly is effectively improved.
In addition, in order to facilitate the assembly of the fixing screw 5, an end head 21 may be coaxially disposed on the fixing screw 5 located outside the gland 3, and a set of opposite side walls of the end head 21 are provided with mutually symmetrical clamping planes 22, so that the fixing screw 5 can be easily penetrated through the gland 3 and the micron-sized filter 2 by using a clamping wrench, and is screwed and fixed on the connecting block 4 on the bottom wall of the mounting cavity 8, so that further fixation of the micron-sized filter 2 can be easily realized.
In actual assembly, first, the first O-ring 12 is assembled in the first annular groove 11, the second O-ring 14 is assembled in the second annular groove 13, the third O-ring 16 is assembled in the third annular groove 15, and the fourth O-ring 20 is assembled in the fourth annular groove 19; then the micron-sized filter 2 is sleeved in the mounting cavity 8 of the filter shell 1 with the opening downwards, so that the lower opening edge of the micron-sized filter 2 is pressed on the first O-shaped sealing ring 12; then the gland 3 is buckled on the upper opening of the filter shell 1; finally, the fixing screw rod 5 passes through the gland 3 and the micron-sized filter 2 from top to bottom and is screwed on the internal thread connecting hole 10 at the center of the top surface of the connecting block 4, so that the gland 3 and the micron-sized filter 2 are further fixed, and the cell filtration device is assembled. After the assembly is completed, the cell culture device can be butted to a harvest liquid pipeline of in-vitro cell culture, namely, the harvest liquid pipeline of in-vitro cell culture is butted to the outer end of the liquid inlet pipe 6, a peristaltic pump is arranged on the harvest liquid pipeline, and meanwhile, the outer end of the liquid discharge pipe 7 is connected with a hose so as to facilitate harvesting of cell suspension.
Claims (8)
1. A cell filtration device, characterized in that: comprising
A filter housing having an upper open mounting cavity;
the micron-sized filter is provided with a filter cavity with a lower opening, the micron-sized filter is sleeved in the mounting cavity in a clearance manner, and the lower opening of the micron-sized filter is sealed and buckled on the bottom wall of the mounting cavity;
a gland, which is sealed on the upper opening of the filter shell in an adaptive way, and the lower surface of the gland is buckled on the top wall of the micron-sized filter;
the connecting block is fixedly connected to the bottom wall of the installation cavity at the inner side of the micron-sized filter, and an internal thread connecting hole is formed in the top wall of the connecting block;
the fixed screw rod sequentially passes through the gland and the micron-sized filter in a sealing way from top to bottom, and the lower end of the fixed screw rod is in fit and screwed connection with the internal thread connecting hole;
the liquid inlet pipe is arranged on the gland, and the inner end of the liquid inlet pipe is communicated with the mounting cavity at the outer side of the micron-sized filter; a kind of electronic device with high-pressure air-conditioning system
The liquid draining pipe is arranged on the bottom wall of the filter shell, and the inner end of the liquid draining pipe is communicated with the filter cavity.
2. The cell filtration device of claim 1, wherein: the filter shell and the micron-sized filter are of cylindrical structures, and the filter shell, the micron-sized filter, the gland, the connecting block, the internal thread connecting hole and the fixing screw are coaxially arranged.
3. The cell filtration device of claim 2, wherein: the bottom wall of the installation cavity at the joint of the lower opening edge of the micron-sized filter is provided with a first annular groove, and a first O-shaped sealing ring is arranged in the first annular groove.
4. The cell filtration device of claim 2, wherein: a second annular groove is formed in the bottom wall of the gland at the joint of the top wall of the micron-sized filter, the center of the second annular groove is consistent with the center of the bottom wall of the gland, and a second O-shaped sealing ring is arranged in the second annular groove.
5. The cell filtration device of claim 2, wherein: a third annular groove is formed in the outer side wall of the gland along the circumferential direction, and a third O-shaped sealing ring is arranged in the third annular groove.
6. The cell filtration device of claim 2, wherein: the center of the top wall of the micron-sized filter is upwards protruded to form a clamping table, and a clamping groove which is matched and clamped with the clamping table is formed in the center of the lower surface of the gland.
7. The cell filtration device of claim 6, wherein: and a fourth annular groove is formed in the inner wall of the perforation at the center of the clamping table, which is attached to the fixing screw, along the circumferential direction, and a fourth O-shaped sealing ring is arranged in the fourth annular groove.
8. The cell filtration device of claim 1, wherein: and one end head is coaxially arranged on the fixing screw rod positioned at the outer side of the gland, and a group of opposite side walls of the end head are provided with clamping planes which are symmetrical to each other.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202320524023.7U CN219449689U (en) | 2023-03-17 | 2023-03-17 | Cell filtration device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202320524023.7U CN219449689U (en) | 2023-03-17 | 2023-03-17 | Cell filtration device |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN219449689U true CN219449689U (en) | 2023-08-01 |
Family
ID=87379892
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202320524023.7U Active CN219449689U (en) | 2023-03-17 | 2023-03-17 | Cell filtration device |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN219449689U (en) |
-
2023
- 2023-03-17 CN CN202320524023.7U patent/CN219449689U/en active Active
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