CN219447773U - Transfer tank for biological sample transfer - Google Patents

Abstract

A transfer pot for biological sample transfer, comprising: the transfer tank is characterized by further comprising: a carrying plate frame for placing the freezing box, wherein the carrying plate frame is fixed below the cover body, an opening is formed in the center of the cover body, and the freezing box is placed on the object carrying plate frame through the opening; the plurality of cold guide columns are fixed below the object carrying plate frame; and the heat insulation layer is filled around the plurality of cold guide columns. The utility model solves the problems of reducing the consumption of the refrigerant and improving the accurate positioning of the sample rack in the running process of the biological sample.

Description

Transfer tank for biological sample transfer

Technical Field

The utility model relates to the technical field of deep low-temperature biological sample storage and transportation, in particular to a transfer tank for biological sample transfer.

Background

With the development of biopharmaceutical technology and the gradual progress of biological sample analysis technology, the requirements on the quality of biological samples are increasing. Generally, a biological sample is subjected to a cryogenic or ultra-low temperature treatment before being stored, and a transfer step is performed after the treatment is completed. During the transfer process, the activity and quality of the biological sample are damaged by improper treatment. A transfer container is often required to safely transfer the biological sample.

The cell biology research shows that the storage temperature of the frozen cells is preferably controlled below minus 130 ℃ all the time, water in the frozen cells can not form crystals, and the cell structure can not be damaged, thereby ensuring the quality of the frozen cells to the maximum extent and leading the survival rate of the recovered cells to be higher. In addition, studies have shown that intracellular metabolism is almost completely stopped when the temperature is reduced to-130 ℃, and that the highly ordered structural state inside the cell is not disturbed, and that intracellular proteins, enzymes and other organelles are not destroyed. After resuscitating, the frozen cells not only have normal cell morphology, but also maintain the whole biological characteristics of the cells. Therefore, the access to the cells in the frozen state must be ensured to be always in an ultralow temperature environment below-130 ℃, and the required quality can be ensured only when the stored frozen cells are subjected to future scientific research or treatment feedback. Once the temperature of the frozen cells is raised from-196 ℃ to a temperature near vitrification, i.e., above-130 ℃, slow and continuous changes occur inside the cell cryopreservation solution, and recrystallization occurs. Most cells form ice crystals intracellularly at-5℃to-60℃and cause cell death, so-5℃to-60℃is called the dangerous temperature zone of the cell.

In the prior art, a heat preservation container filled with liquid nitrogen is generally used as a transit container of a biological sample, and although the low-temperature environment can be ensured, a sample rack of the freezing box cannot realize accurate positioning, so that the biological sample is very inconvenient to take and lift, and the biological sample in the freezing box is easy to damage due to transportation under a long distance or jolt state. Moreover, the existing transfer container needs to be continuously supplemented with liquid nitrogen refrigerant to maintain the low-temperature living environment of the biological sample, and the consumption of the liquid nitrogen refrigerant is very large.

Disclosure of Invention

The utility model aims to solve the problems that the consumption of the refrigerant for maintaining low temperature in the traditional transfer tank for transferring biological samples is overlarge, and a sample rack for placing a freezing box cannot realize accurate positioning.

A transfer pot for biological sample transfer of the present utility model comprises: the transfer tank is characterized by further comprising:

a carrying plate frame for placing the freezing box, wherein the carrying plate frame is fixed below the cover body, an opening is formed in the center of the cover body, and the freezing box is placed on the object carrying plate frame through the opening;

the plurality of cold guide columns are fixed below the object carrying plate frame;

the heat insulation layer is filled around the plurality of cold guide columns;

the tank body is of a double-layer structure, a vacuum heat-preserving cavity is formed between the outer layer wall and the inner layer wall of the tank body, the plurality of cold guide columns and the object carrying plate frame are both positioned in the tank body, a refrigerant is arranged below the object carrying plate frame, and the heat-preserving layer is made of porous materials and is used for absorbing the refrigerant and storing cold energy.

Preferably, the refrigerant is liquid nitrogen, the liquid nitrogen permeates into the heat insulation layer, and a preset gap is reserved between the heat insulation layer and the object carrying plate frame.

Preferably, the object carrying plate frame is fixedly connected with the cover body through a plurality of support columns, the object carrying plate frame comprises a bottom plate and a plurality of boss structures, a cold conducting through hole is formed in the center of the bottom plate, the boss structures are arranged on one side, facing the cover body, of the bottom plate, and the shape and the size of an inner outline surrounded by the boss structures are matched with those of the freezing storage box;

the plurality of boss structures comprise a cold guide column connecting structure, a support column connecting structure and a limiting guide structure, two chamfer structures are respectively arranged on one side of the freezing storage box, and a guide inclined plane of the limiting guide structure is matched with the chamfer structures of the freezing storage box.

Preferably, two lifting lugs are symmetrically arranged at the outer edge of the tank body, and a notch is formed in the bottom of each lifting lug; the transfer pot further comprises: and the handle is movably connected with the lifting lug, and two ends of the handle are respectively provided with a bending part used for being clamped into the notch.

Preferably, a heat insulation film is arranged on the inner surface of the outer layer wall of the tank body, and heat insulation cotton is arranged on the heat insulation film.

Preferably, the bottom of the inner layer wall of the tank body is of a downward-protruding arc-shaped structure.

Preferably, the thermal conductivity of the cover body is smaller than that of the cold guide column.

Preferably, the thermal conductivity of the cover body is 0.02-0.05 w/(m×k), and the thermal conductivity of the cold guide column is 200-300 w/(m×k).

Preferably, the thermal conductivity of the support column is smaller than the thermal conductivity of the cold guide column.

Preferably, the thermal conductivity of the support column is 0.02-0.05 w/(m×k).

Based on the technical scheme, the heat-insulating layer is filled around the cold guide column with larger heat conductivity, so that the heat-insulating layer can transfer the temperature of the refrigerant to the object carrying plate frame for placing the biological sample to the greatest extent, and the biological sample can be always kept under a low-temperature environment condition, so that the consumption of the refrigerant is reduced; and the bottom plate of the object carrying plate frame is provided with the cold guide through holes so that the cold air is directly contacted with the frozen storage box, and the cold guide efficiency is improved. In addition, the bottom of the lifting lug is provided with the notch, and the two ends of the handle are provided with the bending parts, so that the handle is fixed by clamping the bending parts into the notch, and further the transfer tank is effectively prevented from shaking and suddenly falling in the operation process.

Drawings

FIG. 1 is a perspective view of a transfer pot for biological sample transfer according to the present utility model;

FIG. 2 is a front view of a transfer pot for biological sample transfer according to the present utility model;

FIG. 3 is a side view of a transfer pot for biological sample transfer according to the present utility model;

FIG. 4 is a top view of a transfer pot for biological sample transfer according to the present utility model;

FIG. 5 is a side cross-sectional view of a transfer pot for biological sample transfer according to the present utility model;

FIG. 6 is an enlarged schematic view at A in FIG. 2;

FIG. 7 is a perspective view of a carrier rack in a transfer pot for biological sample transfer in accordance with the present utility model;

fig. 8 is a perspective view of a handle in a transfer pot for transferring biological samples according to the present utility model.

Detailed Description

The utility model will be further described with reference to the accompanying drawings.

As shown in fig. 1 to 6, the present utility model, namely, a transfer pot for biological sample transfer, includes:

a vacuum heat preservation cavity 11 is formed between the outer layer wall and the inner layer wall of the tank body 1 with the double-layer structure;

a cover 2;

the object carrying plate frame 4 is positioned in the tank body 1 and used for placing the freezing box 100, is arranged in the tank body 1 and is fixedly connected below the cover body 2 through a plurality of support columns 3;

a plurality of cold guide columns 5 which are positioned in the tank body 1 and are connected below the object carrying plate frame 4 and used for immersing the refrigerant 12, the heat insulation layers 6 are filled around the cold guide columns, and the heat insulation layers 6 are made of porous materials and used for absorbing the refrigerant 12 and storing cold energy;

two lifting lugs 7 symmetrically arranged at the outer edge of the tank body 1; and

a handle 8 movably connected with the lifting lug 7;

specifically, as shown in fig. 7, the carrier rack 4 includes: the bottom plate 42 and a plurality of boss structures 41, bottom plate 42 and a plurality of boss structure 41 integrated into one piece set up, the center of bottom plate 42 is provided with leads cold through hole 421, the temperature of year thing grillage 4 below refrigerant 12 accessible leads cold through hole 421 and directly transmits to the cryopreserved box 100, thereby improved and lead cold efficiency, a plurality of boss structures 41 include lead cold post connection structure 411, support column connection structure 412 and spacing guide structure 413, lead cold post 5 through lead cold post connection structure 411 and year thing grillage 4 fixed connection, support column 3 passes through support column connection structure 412 and year thing grillage 4 fixed connection, be provided with the direction inclined plane on the spacing guide structure 413, the direction inclined plane is used for cooperating the chamfer structure of cryopreserved box 100, avoid the misplacement direction of cryopreserved box 100.

Referring to fig. 4 and 7 in combination, a plurality of boss structures 41 of the object carrying rack 4 are disposed on one side of the bottom plate 42 facing the cover 2, the shape and size of an inner contour surrounded by the boss structures 41 are matched with those of the freezing box 100, and among four corners of the freezing box 100, two adjacent corners are right angles, two other adjacent corners are provided with chamfer structures (i.e. two chamfer structures are respectively disposed on one side of the freezing box 100), and guiding inclined planes of two limiting guide structures 413 are matched with two chamfer structures of the freezing box 100, so that the setting of the boss structures 41 facilitates rapid lifting and accurate positioning of the freezing box 100.

As shown in fig. 5, a notch 71 is formed at the bottom of each lifting lug 7; the refrigerant 12 is liquid nitrogen or dry ice, the liquid nitrogen permeates into the heat preservation layer 6, and a preset gap is reserved between the heat preservation layer 6 and the object carrying plate frame 4.

As shown in fig. 8, two ends of the handle 8 are respectively provided with a bending part 81 for being clamped into the notch 71, and two lower parts of two sides of the handle 8 are respectively provided with a waist hole 82; as shown in fig. 5-6, the handle 8 and the lifting lug 7 can be movably connected by penetrating the screw 9 into the waist hole 82, and when the handle 8 is lifted upwards, the bending part 81 can be clamped into the notch 71 of the lifting lug 7 to be fixed, so that the stability of the transfer pot during transferring is improved.

In this embodiment, the tank 1 has a cylindrical structure with a hollow interior and an open upper end, a heat insulation film (not shown) (such as a mirror aluminum foil) is disposed on the inner surface of the outer wall, heat insulation cotton 10 for heat insulation is disposed on the heat insulation film, and the bottom of the inner wall of the tank 1 has a downward protruding arc structure, so as to avoid deformation during vacuumizing, and increase the accommodating space of the refrigerant 12.

In this embodiment, the thickness of the inner wall of the tank body 1 is uniform, and the upper surface and the lower surface of the bottom of the inner wall are both arc surfaces protruding downward. In other embodiments, the thickness of the inner wall of the can body 1 may be non-uniform, for example, the bottom of the inner wall is set to have a thin middle and a thick edge, the upper surface of the inner wall is still a downwardly convex arc surface, and the lower surface of the bottom of the inner wall is a plane.

In this embodiment, the cover 2 is disposed at the opening at the upper end of the can 1, an opening is disposed at the center of the cover 2, the freezing box 100 is placed on the carrier rack 4 through the opening, the cover 2 is made of a material with a smaller thermal conductivity, and the thermal conductivity ranges from 0.02 w/(m×k) to 0.05 w/(m×k). Similarly, the support columns 3 are also made of a material with a low thermal conductivity, and the thermal conductivity is in the range of 0.02-0.05 w/(m×k). Differently, the thermal conductivity of the cold leg 5 is greater than that of the cover 2 and the support leg 3, with a thermal conductivity ranging from 200 to 300 w/(m×k).

In this embodiment, the heat-insulating layer 6 is made of porous materials such as polyurethane and foam copper as the liquid nitrogen adsorption layer, and is filled around the cold-conducting column 5, so as to realize the heat-insulating and cold-accumulating effects around the cold-conducting column 5.

In this embodiment, the heat-insulating layer 6 is horizontally arranged and has a certain thickness and hardness, and a flowing space of liquid nitrogen is formed between the heat-insulating layer 6 and the arc structure at the bottom of the tank body 1.

In this embodiment, the transfer pot for transferring biological samples further includes an outer cover (not shown in the drawing), which is disposed on the cover 2 and fixedly screwed to the cover 2 or the pot 1. When the biological sample needs long-time storage or transportation, the external environment is isolated by covering the outer cover, so that a closed storage space is formed, and the rapid dissipation of the cold in the transfer tank is avoided.

The application process of the utility model is as follows:

firstly, vacuumizing a vacuum heat preservation cavity 11 formed between an inner layer wall and an outer layer wall of a tank body 1 to reduce heat convection, heat conduction and heat dissipation, coating a heat insulation film on the inner surface of the outer layer wall of the tank body 1, simultaneously placing heat preservation cotton 10 on the heat insulation film, placing a refrigerant 12 (such as dry ice, liquid nitrogen and the like) below a plate carrying frame 4, wherein the liquid level requirement of the refrigerant 12 is lower than the bottom of the plate carrying frame 4 so as to avoid the refrigerant 12 from directly contacting the plate carrying frame 4, thereby not only easily maintaining environmental conditions such as constant humidity and sterility required by biological samples 101 in a freezing box 100, but also improving the utilization rate of the refrigerant 12 and reducing the consumption of the refrigerant 12;

then, the freezing box 100 with the biological sample 101 is placed on the article carrying plate frame 4 from the opening of the cover body 2, and in the process, the article carrying plate frame 4 is provided with the limit guide structure 413 with the guide inclined plane, the cold guide column connecting structure 411 and the support column connecting structure 412, so that the freezing box 100 can be easily placed, taken and fixed, and the freezing box 100 cannot shake on the article carrying plate frame 4 at will;

finally, the handle 8 is lifted vertically, the tank body 1 sinks under the influence of gravity, and at the moment, the bending part 81 of the handle 8 is clamped into the notch 71 at the bottom of the lifting lug 7, so that the transfer tank is not easy to shake in the running process, the risk caused by the sudden landing of the biological sample 101 is reduced, and the overall stability is improved.

In the transferring process, the cold conducting through hole 421 is formed in the middle of the object carrying plate frame 4, so that the temperature can be more directly transferred to the biological sample 101 through the volatilization of the refrigerant 12 at the bottom of the tank body 1, thereby greatly improving the cold conducting efficiency and effectively maintaining the low temperature of the internal environment of the tank body 1; in addition, as the heat conductivity of the cold guide column 5 is larger, and the heat insulation layer 6 is filled around the cold guide column 5, the temperature can be timely transmitted to the biological sample 101 through the loading plate frame 4 by the cold guide column 5, so that the biological sample 101 is always in a low-temperature environment; the biological sample 101 can be well provided with a stable and safe deep low-temperature environment in the two modes; moreover, the support column 3 is made of a material with lower heat conductivity, so that the heat transferred from the outside of the tank body 1 to the object carrying plate frame 4 can be reduced, and the heat exchange between the cover body 2 with lower heat conductivity and the outside air can be well reduced.

Experiments prove that the time for heating the existing transfer container (without the structures such as the cold guide column 5, the heat preservation layer 6 and the like) for biological samples from-196 ℃ to-130 ℃ is generally 2 hours, and the transfer container for transferring biological samples provided by the embodiment has longer heating time and is heated to-130 ℃ from-196 ℃ to more than 6 hours under the same capacity and the same condition. Compared with the prior art, the transfer tank of this application sets up thermal-insulated membrane and heat preservation cotton 10 on the outer wall, sets up cold guide post 5 and heat preservation 6 on the inlayer wall to cooperate with the cold through hole 421 of carrying the thing grillage 4, make carrying thing grillage 4 and carrying the biological sample temperature on the thing grillage 4 lower, consequently can effectively reduce the consumption of refrigerant.

In summary, the utility model has the following advantages:

1. the biological sample can be always kept under a low-temperature environment condition, so that the consumption of the refrigerant is effectively reduced;

2. the placement, extraction and accurate positioning of the freezing box are convenient, and the safety of biological samples in the freezing box in the transportation process is effectively improved;

3. the tank body is kept stable in the transferring process, and the risk caused by sudden landing of the biological sample is reduced;

4. the vacuum tank body is smaller in quality, so that the environment of safety conditions required by biological samples, such as constant humidity sterile conditions, is easier to maintain, and manual transportation is facilitated.

The above embodiments are only for illustrating the technical solution of the present utility model, and not for limiting the same; although the utility model has been described in detail with reference to the foregoing embodiments, it will be understood by those of ordinary skill in the art that: the technical scheme described in the foregoing embodiments can be modified or some or all of the technical features thereof can be replaced by equivalents; such modifications and substitutions do not depart from the spirit of the utility model.

Claims (10)

1. A transfer pot for biological sample transfer, comprising: the transfer tank is characterized by further comprising:

a carrying plate frame for placing the freezing box, wherein the carrying plate frame is fixed below the cover body, an opening is formed in the center of the cover body, and the freezing box is placed on the object carrying plate frame through the opening;

the plurality of cold guide columns are fixed below the object carrying plate frame;

the heat insulation layer is filled around the plurality of cold guide columns;

the tank body is of a double-layer structure, a vacuum heat-preserving cavity is formed between the outer layer wall and the inner layer wall of the tank body, the plurality of cold guide columns and the object carrying plate frame are both positioned in the tank body, a refrigerant is arranged below the object carrying plate frame, and the heat-preserving layer is made of porous materials and is used for absorbing the refrigerant and storing cold energy.

2. The transfer pot for biological sample transfer according to claim 1, wherein the refrigerant is liquid nitrogen, the liquid nitrogen is infiltrated into the heat preservation layer, and a preset gap is formed between the heat preservation layer and the carrier plate frame.

3. The transfer pot for transferring biological samples according to claim 1, wherein the carrier plate frame is fixedly connected with the cover body through a plurality of support columns, the carrier plate frame comprises a bottom plate and a plurality of boss structures, a cooling through hole is formed in the center of the bottom plate, the boss structures are arranged on one side of the bottom plate, which faces the cover body, and the shape and the size of an inner outline surrounded by the boss structures are matched with those of the freezing box;

the plurality of boss structures comprise a cold guide column connecting structure, a support column connecting structure and a limiting guide structure, two chamfer structures are respectively arranged on one side of the freezing storage box, and a guide inclined plane of the limiting guide structure is matched with the chamfer structures of the freezing storage box.

4. The transfer pot for biological sample transfer according to claim 1, wherein two lifting lugs are symmetrically arranged at the outer edge of the pot body, and a notch is formed at the bottom of each lifting lug; the transfer pot further comprises: and the handle is movably connected with the lifting lug, and two ends of the handle are respectively provided with a bending part used for being clamped into the notch.

5. The transfer pot for transferring biological samples according to claim 1, wherein a heat insulation film is arranged on the inner surface of the outer layer wall of the pot body, and heat insulation cotton is arranged on the heat insulation film.

6. The transfer pot for biological sample transfer according to claim 1, wherein the bottom of the inner wall of the pot body is of a downwardly convex arc-shaped structure.

7. The transfer pot for biological sample transfer of claim 1, wherein the thermal conductivity of the cover is less than the thermal conductivity of the cold leg.

8. The transfer pot for biological sample transfer according to claim 7, wherein the thermal conductivity of the cover is 0.02-0.05 w/(m x k), and the thermal conductivity of the cold leg is 200-300 w/(m x k).

9. A transfer pot for biological sample transfer according to claim 3, wherein the support column has a thermal conductivity less than the thermal conductivity of the cold leg.

10. The transfer pot for biological sample transfer according to claim 9, wherein the support column has a thermal conductivity of 0.02-0.05 w/(m x k).