CN219363667U - Embryo thawing and transplanting culture dish combination - Google Patents

Embryo thawing and transplanting culture dish combination Download PDF

Info

Publication number
CN219363667U
CN219363667U CN202320934977.5U CN202320934977U CN219363667U CN 219363667 U CN219363667 U CN 219363667U CN 202320934977 U CN202320934977 U CN 202320934977U CN 219363667 U CN219363667 U CN 219363667U
Authority
CN
China
Prior art keywords
dish body
embryo
fixedly connected
dish
combination
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
CN202320934977.5U
Other languages
Chinese (zh)
Inventor
宁眺
李晶
李地艳
奎华
冉金山
余俊
刘铃艺
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Kunming University
Original Assignee
Kunming University
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Kunming University filed Critical Kunming University
Priority to CN202320934977.5U priority Critical patent/CN219363667U/en
Application granted granted Critical
Publication of CN219363667U publication Critical patent/CN219363667U/en
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02PCLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P60/00Technologies relating to agriculture, livestock or agroalimentary industries
    • Y02P60/20Reduction of greenhouse gas [GHG] emissions in agriculture, e.g. CO2
    • Y02P60/21Dinitrogen oxide [N2O], e.g. using aquaponics, hydroponics or efficiency measures

Landscapes

  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

The utility model relates to the technical field of auxiliary reproduction, and discloses an embryo thawing and transplanting culture dish combination, which comprises an outer dish body, wherein an inner dish body is fixedly connected with the inner part of the outer dish body, two sliding rods are fixedly connected with the inner wall of the outer dish body, sliding blocks are connected with the outer parts Zhou Jun of the two sliding rods in a sliding manner, a rotating shaft is rotatably connected with the middle parts of the two sliding blocks, a fixing plate is fixedly connected with the periphery of the rotating shaft, a pulling piece is fixedly connected with the bottom of the fixing plate, a cover plate is arranged at the upper part of the outer dish body, a plurality of first notches are formed at the edge of the upper part of the outer dish body, an inner cover ring is fixedly connected with the inner part of the cover plate, and a groove is formed between the cover plate and the inner cover ring. According to the utility model, under the mutual matching of the structures of the slide bar, the slide block, the rotating shaft, the fixed plate, the poking plate and the like, the liquid paraffin of the inner dish body higher than the edge opening can be scraped, so that the liquid paraffin remained on the surface of the embryo is reduced, the adhesion and the residue of the embryo are reduced, and the risk in embryo transfer is further reduced.

Description

Embryo thawing and transplanting culture dish combination
Technical Field
The utility model relates to the technical field of assisted reproduction, in particular to an embryo thawing and transplanting culture dish combination.
Background
Embryo assisted reproduction is to freeze and preserve an embryo, then to perform in vitro fertilization through an embryo transfer technology, and when the embryo needs to be used, the embryo needs to undergo a series of processes of thawing, laser assisted hatching and embryo transfer, and after the embryo is thawed, the embryo needs to be placed in a culture dish for laser assisted hatching.
After the auxiliary incubation of laser is accomplished, need draw the embryo through the transfer line, but can exist the liquid paraffin that is used for the culture in the culture dish, these liquid paraffin can get into the transfer line together with the embryo, can increase embryo adhesion, remain in the risk in the pipe, after the embryo thawing, need place the embryo and observe for a period of time under normal atmospheric temperature, thereby know the activity of embryo, the leakproofness of current culture dish is relatively poor simultaneously, when keeping stand the observation to the embryo, it enters into the culture dish to cause outside bacterium easily, thereby cause the inside infection of embryo culture dish, influence embryo's quality and survival rate, for this reason propose embryo thawing transfer culture dish combination and solve above-mentioned problem.
Disclosure of Invention
The utility model aims to solve the defects in the prior art and provides an embryo thawing and transplanting culture dish combination.
In order to achieve the above purpose, the present utility model adopts the following technical scheme: embryo thawing transplants culture dish combination, including outer ware body, the internal fixedly connected with internal ware body of outer ware, two slide bars of external wall fixedly connected with of outer ware, two Zhou Jun sliding connection has the slider outside the slide bar, two the slider middle part rotates and is connected with the pivot, pivot periphery fixedly connected with fixed plate, fixed plate bottom fixedly connected with plectrum, external ware body upper portion is provided with the apron.
As a further description of the above technical solution:
the inner cover is characterized in that a plurality of first notches are formed in the edge opening on the upper portion of the outer dish body, the inner cover ring is fixedly connected inside the cover plate, a groove is formed between the cover plate and the inner cover ring, the outer dish body is arranged in the middle of the groove, and a plurality of second notches are formed in the edge opening on the bottom of the inner cover ring.
As a further description of the above technical solution:
the middle part of the inner dish body is connected with a plurality of plugboards in a sliding way, and a sealing groove and a sealing gasket are arranged at the joint between the plugboards and the inner dish body.
As a further description of the above technical solution:
the inner part of the outer dish body is fixedly connected with a plurality of secondary dish bodies II and primary dish bodies I, and the secondary dish bodies I and the secondary dish bodies II are different in size.
As a further description of the above technical solution:
the edge opening at the upper part of the inner dish body is lower than the edge opening of the outer dish body, and the poking plate is positioned above the inner dish body.
As a further description of the above technical solution:
the outer ring is fixedly connected to the bottom of the outer periphery of the outer dish body, and anti-skid patterns are arranged on the outer side of the outer ring.
As a further description of the above technical solution:
the two ends of the rotating shaft penetrate through the sliding block and are fixedly connected with rotating plates.
As a further description of the above technical solution:
the outer dish body, the outer ring, the secondary dish body II and the inner dish body are integrally formed, and the edge of the bottom of the inner dish body is not contacted with the bottom of the outer dish body.
The utility model has the following beneficial effects:
according to the utility model, under the mutual matching of the structures of the slide bar, the slide block, the rotating shaft, the fixed plate, the poking plate and the like, the liquid paraffin of the inner dish body higher than the edge opening can be scraped, so that the liquid paraffin remained on the surface of the embryo is reduced, the adhesion and the residue of the embryo are reduced, and the risk in embryo transfer is further reduced.
According to the utility model, the upper part of the outer dish body can be plugged under the mutual matching of the cover plate, the groove, the inner cover ring, the notch II, the notch I and the like, so that the gap for entering outside air is reduced, the probability of entering virus and bacteria into the inner dish body is reduced, and the survival rate of embryos is improved.
Drawings
FIG. 1 is a schematic perspective view of an embryo thawing transfer culture dish assembly according to the present utility model;
FIG. 2 is a schematic diagram of the structure of the inner vessel body of the embryo thawing transfer culture vessel assembly according to the present utility model;
FIG. 3 is a schematic diagram of a cover plate of an embryo thawing transfer culture dish assembly according to the present utility model;
FIG. 4 is a schematic diagram of a pulling plate of an embryo thawing transfer culture dish assembly according to the present utility model;
fig. 5 is a sectional view showing the structure of an embryo thawing transfer culture dish assembly according to the present utility model.
Legend description:
1. an outer vessel body; 2. an outer ring; 3. a cover plate; 4. an inner dish body; 5. inserting plate; 6. a slide bar; 7. a slide block; 8. a rotating shaft; 9. a fixing plate; 10. a pulling piece; 11. a rotating plate; 12. a first secondary dish body; 13. secondary dish body II; 14. a notch I; 15. an inner cover ring; 16. a groove; 17. a second notch; 18. anti-skid lines.
Detailed Description
The following description of the embodiments of the present utility model will be made clearly and completely with reference to the accompanying drawings, in which it is apparent that the embodiments described are only some embodiments of the present utility model, but not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the utility model without making any inventive effort, are intended to be within the scope of the utility model.
Referring to fig. 1-5, one embodiment provided by the present utility model is: embryo thawing transplants culture dish combination, including outer ware body 1, the inside fixedly connected with inner ware body 4 of outer ware body 1, thawing and transplanting the operation are carried out to the embryo through inner ware body 4, outer ware body 1 inner wall fixedly connected with two slide bars 6, restrict slide bar 7's position through slide bar 6, zhou Jun sliding connection has slide 7 outside two slide bars 6, two slide bar 7 middle part rotations are connected with pivot 8, can drive fixed plate 9 through pivot 8 and rotate, thereby adjust the inclination of plectrum 10, with this adjustment and inner ware body 4's contact point, pivot 8 periphery fixedly connected with fixed plate 9, fixed plate 9 bottom fixedly connected with plectrum 10, to the inside additional culture solution that adds of inner ware body 4, make the liquid level of culture solution be higher than inner ware body 4's edge mouth, under the effect of tension, the culture solution can be slightly higher than inner ware body 4 edge mouth, then move through stirring fixed plate 9 drive plectrum 10, can scrape the culture solution that inner ware body 4 is higher than edge mouth, because liquid paraffin can drive fixed plate 9 and rotate, thereby, can carry the embryo in the inner ware body 1 is carried in the inner ware body 1 again in the volume of removing paraffin, and then the inner ware body is reduced, and the inner ware is carried in the volume is more than the volume 1, and the inner ware is reduced and the inner ware is carried in the volume is more than the volume 1, and is more than the whole and is more than reduced and more than the embryo is more than high than 1.
The upper edge of the outer dish body 1 is provided with a plurality of notches I14, after the cover plate 3 covers the outer dish body 1, external air can enter the inner part of the outer dish body 1 from the notches I14, the inner part of the cover plate 3 is fixedly connected with an inner cover ring 15, a groove 16 is formed between the cover plate 3 and the inner cover ring 15, the outer dish body 1 is arranged in the middle of the groove 16, the outer dish body 1 is shielded by the inner cover ring 15, so that the moving distance of the external air is increased, the entry amount of dust and bacteria carried in the air is reduced, the bottom edge of the inner cover ring 15 is provided with a plurality of notches II 17, the air can enter the inner part of the outer dish body 1 from the notches II 17, so that the air entering gap is reduced, the probability of entering the inner dish body 4 by virus bacteria is reduced, the survival rate of the embryo is improved, a plurality of plugboards 5 are slidingly connected in the middle part of the inner dish body 4, a sealing groove and a sealing gasket are arranged at the joint between the plugboard 5 and the inner dish body 4, the outer side of the inner dish body 4 can be opened through the plugboard 5, so that the culture solution in the inner dish body 4 enters the outer dish body 1 from the plugboard 5, the liquid level of the culture solution in the inner dish body 4 is reduced, the inner part of the outer dish body 1 is fixedly connected with a plurality of secondary dish bodies II 13 and 12, the secondary dish bodies II and 12 are different in size, embryos can be temporarily stored through the secondary dish bodies II and 12, then transplanting operation is carried out through the inner dish body 4, the secondary dish bodies II and 12 are different in size and used for classifying different embryo, the edge opening at the upper part of the inner dish body 4 is lower than the edge opening of the outer dish body 1, the poking piece 10 is positioned above the inner dish body 4, the poking piece 10 can be driven to move through the poking fixing plate 9, the culture solution of the inner dish body 4 which is higher than the floating edge opening can be scraped, due to the fact that liquid paraffin is on the surface of the culture solution, can scrape the liquid paraffin and scrape into the outer ware body 1 inside when getting, can further reduce embryo surface remaining liquid paraffin, and then when transplanting the embryo, reduce the adhesion and the residue of embryo, and then reduce the risk in the embryo transfer, outer ware body 1 periphery bottom fixedly connected with outer loop 2, the outer loop 2 outside is provided with anti-skidding line 18, can be convenient for take the culture dish through anti-skidding line 18, slider 7 and fixedly connected with revolving plate 11 are run through at the both ends of pivot 8, can conveniently rotate fixed plate 9 through revolving plate 11, thereby distance between quick adjustment plectrum 10 and the interior ware body 4, outer ware body 1, outer loop 2, inferior ware body two 13, interior ware body 4 integrated into one piece, increase the intensity between the structure, and interior ware body 4 bottom border and the interior bottom of outer ware body 1 are contactless, reduce the direct conduction of heat, thereby ensure the temperature rise to the inside cultivation of interior ware body 4 more even.
Working principle: when in use, the culture solution is added into the outer dish body 1 and the inner dish body 4, then the embryo to be thawed is placed into the inner dish body 4, the heated culture solution is added between the outer dish body 1 and the inner dish body 4, the heat of the heated culture solution is conducted into the inner dish body 4 in a water bath mode, so that the temperature of the culture solution in the inner dish body 4 is raised, the thawing of the embryo is completed, a gap exists between the outer dish body 1 and the bottom of the inner dish body 4, the direct conduction of the heat is reduced, the temperature rise of the culture in the inner dish body 4 is ensured to be more uniform, after the thawing, the embryo can be moved into the secondary dish body 1 or the secondary dish body 13 by using an egg transferring needle, then the culture solution is additionally added into the inner dish body 4, the liquid level of the culture solution is higher than the edge of the inner dish body 4, under the action of tension, the culture solution can be slightly higher than the edge of the inner dish body 4, then the stirring plate 9 is stirred to drive the stirring plate 10 to move, the culture solution of the inner dish body 4 higher than the edge can be scraped, the liquid paraffin floats on the surface of the culture solution, the liquid paraffin can be scraped into the inner part of the outer dish body 1 during scraping, the inserting plate 5 and the bottom of the inner dish body 4 are opened by pumping the inserting plate 5, the liquid level of the culture solution in the inner dish body 4 can be lowered, then the embryo in the first secondary dish body 12 or the second secondary dish body 13 is moved into the inner dish body 4 again, after the liquid paraffin on the surface of the embryo enters into the inner dish body 4, the liquid paraffin can be dispersed under the action of the culture solution, then the liquid paraffin is separated by the method, so that the liquid paraffin remained on the surface of the embryo can be further reduced, and the adhesion and the residue of the embryo can be reduced during embryo transplantation, and then reduce the risk in embryo transfer, make its use more convenient, simultaneously, when keeping stand to observe the embryo, cover plate 3 lid in outer ware body 1 upper portion, then place outer ware body 1 along the mouth at recess 16 middle part, thereby accomplish the seal to the culture dish, outside air can enter into inside the ware body 4 from breach one 14 and breach two 17 simultaneously, with this space that reduces the air and get into, reduce the probability that the virus bacterium enters into inside ware body 4, thereby improve the survival rate of embryo.
Finally, it should be noted that: the foregoing description is only illustrative of the preferred embodiments of the present utility model, and although the present utility model has been described in detail with reference to the foregoing embodiments, it will be apparent to those skilled in the art that modifications may be made to the embodiments described, or equivalents may be substituted for elements thereof, and any modifications, equivalents, improvements or changes may be made without departing from the spirit and principles of the present utility model.

Claims (8)

1. Embryo thawing transplants culture dish combination, including outer ware body (1), its characterized in that: the novel multifunctional household dish is characterized in that the inner dish body (4) is fixedly connected with the inner dish body (1), the two sliding rods (6) are fixedly connected with the inner wall of the outer dish body (1), the two sliding rods (6) are externally Zhou Jun and slidably connected with the sliding blocks (7), the rotating shafts (8) are rotatably connected with the middle parts of the sliding blocks (7), the fixing plates (9) are fixedly connected with the periphery of the rotating shafts (8), the pulling plates (10) are fixedly connected with the bottoms of the fixing plates (9), and the cover plates (3) are arranged on the upper parts of the outer dish body (1).
2. The combination of embryo defrost transfer dishes according to claim 1, wherein: the novel inner cover plate is characterized in that a plurality of first notches (14) are formed in the edge opening on the upper portion of the outer plate body (1), an inner cover ring (15) is fixedly connected inside the cover plate (3), a groove (16) is formed between the cover plate (3) and the inner cover ring (15), the outer plate body (1) is arranged in the middle of the groove (16), and a plurality of second notches (17) are formed in the edge opening on the bottom of the inner cover ring (15).
3. The combination of embryo defrost transfer dishes according to claim 1, wherein: the middle part of the inner dish body (4) is slidably connected with a plurality of plugboards (5), and a sealing groove and a sealing gasket are arranged at the joint between the plugboards (5) and the inner dish body (4).
4. The combination of embryo defrost transfer dishes according to claim 1, wherein: the inner part of the outer dish body (1) is fixedly connected with a plurality of second dish bodies (13) and first dish bodies (12), and the first dish bodies (12) and the second dish bodies (13) are different in size.
5. The combination of embryo defrost transfer dishes according to claim 1, wherein: the edge opening at the upper part of the inner dish body (4) is lower than the edge opening of the outer dish body (1), and the poking plate (10) is positioned above the inner dish body (4).
6. The combination of embryo defrost transfer dishes according to claim 1, wherein: the outer ring (2) is fixedly connected to the bottom of the periphery of the outer dish body (1), and anti-skid patterns (18) are arranged on the outer side of the outer ring (2).
7. The combination of embryo defrost transfer dishes according to claim 1, wherein: the two ends of the rotating shaft (8) penetrate through the sliding block (7) and are fixedly connected with rotating plates (11).
8. The combination of embryo defrost transfer dishes according to claim 6, wherein: the outer dish body (1), the outer ring (2), the secondary dish body (13) and the inner dish body (4) are integrally formed, and the bottom edge of the inner dish body (4) is not contacted with the inner bottom of the outer dish body (1).
CN202320934977.5U 2023-04-24 2023-04-24 Embryo thawing and transplanting culture dish combination Active CN219363667U (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN202320934977.5U CN219363667U (en) 2023-04-24 2023-04-24 Embryo thawing and transplanting culture dish combination

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN202320934977.5U CN219363667U (en) 2023-04-24 2023-04-24 Embryo thawing and transplanting culture dish combination

Publications (1)

Publication Number Publication Date
CN219363667U true CN219363667U (en) 2023-07-18

Family

ID=87144736

Family Applications (1)

Application Number Title Priority Date Filing Date
CN202320934977.5U Active CN219363667U (en) 2023-04-24 2023-04-24 Embryo thawing and transplanting culture dish combination

Country Status (1)

Country Link
CN (1) CN219363667U (en)

Similar Documents

Publication Publication Date Title
CN219363667U (en) Embryo thawing and transplanting culture dish combination
CN106852292B (en) High density aquaculture pond suspended solid collection device
CN213044815U (en) Multilayer three-dimensional fly maggot breeding device
CN217136221U (en) Seed breeding is with contrast cultivation device
CN110885258A (en) Fermentation device using cultivation waste as raw material
CN108552102A (en) A kind of automatic breeding unit
CN208770886U (en) Integrated suspension freezing concentrating device
CN220977009U (en) Carbon dioxide incubator convenient to maintenance
CN214244471U (en) Embryo culture and operation integrated device
CN107155980A (en) Triplophysa cultivates hatching apparatus
CN209527542U (en) A kind of zebra fish fertilized egg collection device
CN108377952A (en) A kind of aquaculture pond excrement cleaning plant used for aquiculture
CN208667686U (en) A kind of fermented tea installation for fermenting
CN216567455U (en) Novel edible mushroom liquefaction fermentation equipment
CN220284037U (en) Lactic acid bacteria fermentation tank
CN220745964U (en) Amplifying device for stem cells
CN205874452U (en) Move plant cell culture device
CN216845873U (en) Cooling tower capable of easily cleaning algae breeding in tower
CN220441567U (en) Pet transfer box
CN219146476U (en) Mandarin fish breeds case
CN219297472U (en) Mould culture apparatus
CN217628335U (en) Novel facility suitable for photosynthetic bacteria automated production
CN212697249U (en) A drainage device that is used for rice shrimp to breed altogether
CN219091225U (en) Ecological rice-fish symbiotic circulating water equipment
CN213963470U (en) Gynaecology uses tumour sample storage box

Legal Events

Date Code Title Description
GR01 Patent grant
GR01 Patent grant