CN219363662U - Up-down magnetic stirring type bioreactor - Google Patents

Abstract

The utility model discloses an up-down magnetic stirring type bioreactor for solving the problem of insufficient sealing effect of a driving mode in the field of cell culture or bio-pharmacy, which comprises the following components: the device comprises a liquid storage container, an upper magnetic stirring assembly and a lower magnetic stirring assembly; the upper magnetic stirring assembly and the lower magnetic stirring assembly both comprise: a seal housing assembly; the first magnetic force rotating piece is arranged in the sealing shell assembly; the second magnetic force rotating piece is arranged outside the sealing shell assembly and is provided with stirring paddles; the driving assembly is connected with the first magnetic rotating piece and can drive the first magnetic rotating piece to rotate, and the first magnetic rotating piece can drive the second magnetic rotating piece to rotate. The mode of driving the second magnetic force rotating piece through the magnetic force of the first magnetic force rotating piece is used for isolating the power part and the transmission part of the stirring part from the space in the liquid storage container better, so that the problem of insufficient sealing effect in the existing mechanical driving mode is avoided.

Description

Up-down magnetic stirring type bioreactor

Technical Field

The utility model relates to the field of cell culture or bio-pharmaceuticals, in particular to an up-down magnetic stirring type bioreactor.

Background

Cell culture refers to a method of simulating in vitro an in vivo environment to survive, grow, reproduce and maintain primary structure and function.

The bioreactor is a production experimental device for providing a reaction environment for cell culture, pharmaceutical production, animal and plant tissue culture, microbial fermentation production, biological product preparation, sewage treatment and other experiments, and is sometimes also called a fermenter or reactor.

With the continuous development of cell culture technology, the wide application of bioreactors has been a major trend in the fields of cell culture and pharmacy, and with the continuous improvement of requirements for safety, convenience, high efficiency and the like of cell culture processes in the fields, the application of disposable systems has been over 30 years old, in China, the application of disposable systems is relatively late in starting, but has developed rapidly in recent years, a batch of biopharmaceutical enterprises have introduced disposable systems into pharmaceutical processes, and at present, the disposable systems in the pharmaceutical fields have been widely applied to the processes of cell culture/fermentation, preparation and storage of culture media and buffer solutions, and to the processes of storage and transfer of process materials, freeze thawing of final products, raw solutions and semi-finished products, filling, sampling and the like.

Disposable bioreactors are often used in disposable systems, and most of the containers of disposable bioreactors are bags, and most of the containers in the market have a capacity of 3L to 2000L, and also have reactors with larger capacities, such as 6000L.

At present, the general disposable bioreactor generally adopts a stirring assembly to realize the sealing between the parts of the stirring assembly and the container in a mechanical sealing mode, and the sealing mode easily causes leakage of culture solution in the container, so that the normal use of the reactor is affected, and the influence of cultured cells on the outside, such as bacteria contamination risk, cannot be effectively controlled.

Disclosure of Invention

The utility model provides a bioreactor, which aims at solving at least one of the technical problems existing in the prior art.

The technical scheme of the utility model provides an upper and lower magnetic stirring type bioreactor, which comprises the following components:

the device comprises a liquid storage container, an upper magnetic stirring assembly and a lower magnetic stirring assembly;

the upper magnetic stirring assembly and the lower magnetic stirring assembly both comprise:

a seal housing assembly;

the first magnetic force rotating piece is arranged in the sealing shell assembly;

the second magnetic force rotating piece is arranged outside the sealing shell assembly and is provided with stirring paddles;

the driving assembly is connected with the first magnetic rotating piece and can drive the first magnetic rotating piece to rotate, and the first magnetic rotating piece can drive the second magnetic rotating piece to rotate;

the sealing shell component of the upper magnetic stirring component is connected with the inner wall of the upper end of the liquid storage container, and the sealing shell component of the lower magnetic stirring component is connected with the inner wall of the lower end of the liquid storage container.

Further, the seal housing assembly includes:

a first tube body;

the installation shell is connected with the second end of the first pipe body, the first magnetic force rotating piece is arranged in the installation shell, and the second magnetic force rotating piece is sleeved on the first pipe body or the installation shell;

the first end of the first pipe body of the upper magnetic stirring assembly is connected with the inner wall of the upper end of the liquid storage container, and the first end of the first pipe body of the lower magnetic stirring assembly is connected with the inner wall of the lower end of the liquid storage container.

Further, a top disk is arranged at the first end of the first pipe body of the upper magnetic stirring assembly and is connected with the inner wall of the upper end of the liquid storage container in a hot melting mode;

the first end of the first pipe body of the lower magnetic stirring assembly is provided with an aeration disc, and the aeration disc is connected with the inner wall of the lower end of the liquid storage container in a hot melting mode.

Further, the drive assembly includes:

the middle part of the driving shaft is arranged in the first pipe body, and the second end of the driving shaft is inserted into the installation shell and connected with the first magnetic force rotating piece.

Further, the upper magnetic stirring assembly and the lower magnetic stirring assembly each further comprise:

the middle part of the driving shaft is arranged in the second pipe body.

Further, the drive assembly further comprises:

the first end of the second pipe body is connected with the mounting plate in a threaded mode, and the second end of the second pipe body is connected with the mounting shell in a threaded mode;

the driving piece is arranged on the mounting plate, the first end of the driving shaft penetrates through the mounting plate and is connected with the driving piece, and the driving piece can drive the driving shaft to rotate;

the first end of the driving shaft is provided with a connecting sleeve, and the output end of the driving piece is connected with the connecting sleeve in a key connection mode.

Further, the first tube body is a silicone tube;

the first end of the installation shell comprises an installation tube part, and the second magnetic force rotating piece is sleeved on the installation tube part;

the seal housing assembly further includes:

the first end of the middle connecting pipe is connected with the second end of the silicone tube, and the second end of the middle connecting pipe is connected with the mounting tube part;

the second magnetic rotating piece of the upper magnetic stirring assembly is supported by the mounting pipe portion of the upper magnetic stirring assembly, and the second magnetic rotating piece of the lower magnetic stirring assembly is supported by the middle connecting pipe of the lower magnetic stirring assembly.

Further, the first end of the middle connecting pipe is provided with an annular convex buckle, and the annular convex buckle can be buckled into the second end of the silicone tube;

the second end of the middle connecting pipe is connected with the mounting pipe part in a threaded mode.

Further, the first magnetic rotator includes:

the first rotating shell is arranged in the mounting shell;

the first magnetic piece is arranged in the first rotating shell;

the first end of the first rotating shell is connected with the inner wall of the first end of the mounting shell in a bearing way;

the second end of the first rotating shell is connected with the inner wall of the second end of the mounting shell in a bearing mode.

Further, the second magnetic rotator includes:

the second rotating shell is sleeved on the mounting shell;

the second magnetic piece is arranged in the second rotary shell;

the second rotating shell is connected with the mounting shell through a bearing mode.

The beneficial effects of the utility model are as follows:

according to the up-down magnetic stirring type bioreactor, the second magnetic rotating piece is driven by the magnetic force of the first magnetic rotating piece, and the power part and the transmission part of the stirring part are isolated from the space in the liquid storage container better, so that the problem of insufficient sealing effect in the existing mechanical driving mode is avoided;

the upper magnetic stirring assembly and the lower magnetic stirring assembly are used for covering different stirring spaces of the large-capacity bioreactor such as 6000L and the like, so that the culture solution in the lower part of the liquid storage container can be ensured to fully react, the culture solution in the upper part of the liquid storage container can be ensured to fully react, the stirring effect is better, the stirring range is larger, the traditional through driving shaft can be omitted, the liquid storage container is convenient to fold, the packaging, the transportation and the field installation of the bioreactor are convenient, no metal material can be selected for isolating the materials in and out of the storage container, the negative influence of metal on the inside of the bioreactor is avoided, and the bioreactor is easier to realize a sterile environment.

Drawings

FIG. 1 is a schematic diagram of an embodiment of an up-down magnetic stirring bioreactor;

FIG. 2 is a schematic structural view of a magnetic stirring assembly embodiment at a cross-sectional view 1;

FIG. 3 is a schematic structural view of a magnetic stirring assembly embodiment at a cross-sectional view 2;

fig. 4 is an enlarged schematic view of the portion a in fig. 3.

Reference numerals: the liquid storage container 100, the vent disc 110, the first tube 210, the top disc 220, the mounting plate 230, the mounting housing 300, the mounting tube portion 310, the first magnetic rotator 400, the driving shaft 510, the connecting sleeve 520, the second magnetic rotator 600, the stirring blade 700, the second tube 800, the intermediate connecting tube 910, and the annular protruding buckle 920.

Detailed Description

The following description will describe several embodiments of the present utility model, including the embodiments corresponding to the accompanying drawings, it being understood that the drawings are for aiding in the understanding of the technical features and technical solutions of the present utility model, and should not be construed as limiting the scope of the present utility model.

The conception, specific structure and technical effects of the present utility model will be clearly and completely described in the following with reference to the embodiments and the drawings, to fully understand the objects, aspects and effects of the present utility model. It should be noted that, in the case of no conflict, the embodiments and features in the embodiments may be combined with each other.

It should be noted that, unless explicitly defined otherwise, when a certain feature is referred to as being "fixed," "connected," or "mounted" on another feature, it may be directly fixed or connected to the other feature or may be indirectly fixed or connected to the other feature, and the terms such as "fixed," "connected," or "mounted" should be interpreted broadly, so that those skilled in the art can reasonably determine the specific meaning of the terms in the present utility model in combination with the specific contents of the technical scheme.

It should be noted that, the description of the orientation or positional relationship indicated by the upper, lower, left, right, top, bottom, front, rear, inner, outer, first end, second end, etc. used in the present utility model is based on the orientation or positional relationship of the drawings or the embodiments, only for convenience of describing the present utility model and simplifying the description, and does not indicate or imply that the apparatus or elements referred to must have a specific orientation, be configured and operated in a specific orientation, and thus should not be construed as limiting the present utility model.

It is noted that the term "and/or" as used in the present utility model includes any combination of one or more of the listed items, meaning one or more, meaning at least two, greater than, less than, exceeding, etc. are understood to exclude this number, and the above, below, within, etc. are understood to include this number.

The rotation conversion rotating member defined in the utility model can rotate around the axis of the rotation conversion rotating member and can be driven to rotate by other power members or transmission members.

It should be noted that, if the first and second descriptions are only used for distinguishing technical features in the present utility model, the description should not be construed as indicating or implying relative importance or implying the number of the indicated technical features or implying the precedence relationship of the indicated technical features.

It is to be understood that all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art unless explicitly defined otherwise. The terminology used in the description presented herein is for the purpose of describing particular embodiments only and is not intended to be limiting of the utility model.

The basic embodiment of the present utility model provides an up-down magnetic stirring bioreactor, referring to fig. 1 to 4, comprising:

the device comprises a liquid storage container, an upper magnetic stirring assembly and a lower magnetic stirring assembly;

the upper magnetic stirring assembly and the lower magnetic stirring assembly both comprise:

a seal housing assembly;

the first magnetic force rotating piece is arranged in the sealing shell assembly;

the second magnetic force rotating piece is arranged outside the sealing shell assembly and is provided with stirring paddles;

the driving assembly is connected with the first magnetic rotating piece and can drive the first magnetic rotating piece to rotate, and the first magnetic rotating piece can drive the second magnetic rotating piece to rotate;

the sealing shell component of the upper magnetic stirring component is connected with the inner wall of the upper end of the liquid storage container, and the sealing shell component of the lower magnetic stirring component is connected with the inner wall of the lower end of the liquid storage container.

The sealed shell assembly can be regarded as including first body, middle connecting pipe and the upper concept of installation casing, can set up first magnetic force rotation piece including, at the second magnetic force rotation piece of setting up, make two magnetic force rotation pieces separate, the rethread can be regarded as including drive shaft, drive piece and the drive assembly of the upper concept of mounting panel and drive first magnetic force rotation piece rotation, and can drive second magnetic force rotation piece rotation through first magnetic force rotation piece rotation, can realize the isolation installation of second magnetic force rotation piece and drive assembly, when making second magnetic force rotation piece can normally stir the culture solution in the stock solution container, second magnetic force rotation piece and drive assembly can not influence the inside of stock solution container under sealed shell assembly's isolation effect.

Different stirring spaces of the large-capacity bioreactor such as 6000L are covered through the upper magnetic stirring assembly and the lower magnetic stirring assembly, the stirring effect is better, the stirring range is larger, a traditional through driving shaft can be omitted, the liquid storage container is convenient to fold, and the bioreactor is convenient to package, transport and install on site.

Development of the basic embodiment referring to fig. 1-4, a magnetic stirring assembly comprises:

the first pipe body 210 is arranged in the liquid storage container 100, a first end of the first pipe body 210 is connected with the inner wall of the liquid storage container 100, further, the first pipe body 210 and the liquid storage container 100 can be both provided with deformable structures, wherein the liquid storage container 100 can be formed by heat sealing high-barrier multilayer co-extrusion films meeting pharmaceutical standards, and the liquid storage container 100 can be folded for convenient transportation and installation;

the installation housing 300 is connected to the second end of the first pipe body 210, the installation housing 300 is used for protecting the first magnetic rotating member 400, and further, the installation housing 300 may include two parts, one part is used for internally installing the first magnetic rotating member 400, and the other part is used for connecting the first pipe body 210 and installing the second magnetic rotating member 600;

the first magnetic force rotating member 400 is disposed in the installation housing 300;

the driving shaft 510, the middle part of which is disposed in the first pipe body 210, and the second end of the driving shaft 510 is inserted into the mounting housing 300 and connected with the first magnetic rotating member 400, it is understood that the middle part is not limited to the middle position, and it is understood that all or part of the middle area of the two ends is the middle part, and the second end of the driving shaft 510 can be connected with the first magnetic rotating member 400 by a general transmission connection manner such as a key connection manner, a screw connection manner, etc.;

the second magnetic rotating member 600 is sleeved on the first pipe body 210 or the mounting shell 300, the second magnetic rotating member 600 is provided with a stirring blade 700, further, the stirring blade 700 can be integrally formed with the shell of the second magnetic rotating member 600, and can also be arranged on the second magnetic rotating member 600 through a general mounting and fixing structure, and the second magnetic rotating member 600 can be positioned on one axial side of the first magnetic rotating member 400, and can also be positioned on one radial side of the first magnetic rotating member 400;

the rotation of the driving shaft 510 can drive the first magnetic rotator 400 to rotate, and the rotation of the first magnetic rotator 400 can drive the second magnetic rotator 600 to rotate.

The bioreactor of the basic embodiment drives the first magnetic rotating member 400 to rotate through the driving shaft 510, and drives the second magnetic rotating member 600 to rotate by utilizing the magnetic action of the first magnetic rotating member 400, and the inner cavities of the driving shaft 510 and the liquid storage container 100 are isolated by the first pipe body 210 matched with the installation shell 300, so that the bacterial contamination risk caused by the traditional mechanical driving and stirring and the influence of the metal parts of the driving part on the inside of the liquid storage container 100 are avoided, the sterility assurance capability of the bioreactor is improved, and the liquid storage container 100 can be conveniently folded and packaged and can conveniently meet the irradiation sterilization requirement.

Through the structural arrangement of the bioreactor of the basic embodiment, the driving shaft 510 can be free from being arranged into a long stirring shaft structure, so that the liquid storage container 100 can be folded and transported, and the installation process can ensure the tightness of the liquid storage container 100 after the components in the liquid storage container 100 such as the first pipe body 210, the installation shell 300, the first magnetic rotating member 400, the second magnetic rotating member 600 and the like are arranged, so that the driving shaft 510 is inserted into the first pipe body 210, and the driving shaft 510 is connected with the first magnetic rotating member 400 in a transmission way.

The stirring blade is driven by a magnetic driving mode, so that the biological cell culture of each capacity can be met, the damage to cells is reduced under the condition of low-shear force stirring, and meanwhile, the effect of high mixing is achieved under the condition of low-rotation-speed stirring. In particular 6000L bioreactor is able to culture more cells in the same cell culture time.

Development of the basic embodiment referring to fig. 2-4, the magnetic stirring assembly further comprises:

the second tube 800 is disposed in the middle of the first tube 210, and the driving shaft 510 is disposed in the middle of the second tube 800, which is understood to be not limited to a middle position, but may be understood to be a middle part of all or part of the middle of two ends, and the second tube 800 is disposed to facilitate protection of the driving shaft 510 and also facilitate filling of a gap between the driving shaft 510 and the first tube 210.

2-4, the first tube 210 is a silicone tube, the second tube 800 is a stainless steel tube, the silicone tube is configured to facilitate folding of the liquid storage container 100, the silicone tube is also a preferred material for isolating the liquid storage container 100 from the driving shaft 510, the stainless steel tube is configured to facilitate the soft silicone tube to be straightened as much as possible by installing the stainless steel tube before installing the driving shaft 510, so that the driving shaft 510 is installed and positioned more accurately when the driving shaft 510 is installed, and the first magnetic rotating member 400 can be connected more conveniently.

It will be appreciated that the first tube may be made of other flexible materials that can meet the cell culture requirements, and the second tube may be made of other rigid materials that can meet the cell culture requirements.

Further development of the basic embodiment, referring to fig. 2-4, the magnetic stirring assembly further comprises:

a mounting plate 230 disposed outside the liquid storage container 100;

the first end of the second tube 800 is connected with the mounting plate 230 in a threaded manner, the second end of the second tube 800 is connected with the mounting housing 300 in a threaded manner, the second tube 800 is fixed in a threaded manner at both ends, the mounting and positioning of the driving shaft 510 can be completed better, the mounting space of the driving shaft 510 can be well defined by limiting the inner pipe diameter of the second tube 800 to be slightly larger than the shaft diameter of the driving shaft 510 in consideration of the variability of the silicone pipe, and the driving shaft 510 is connected with the first magnetic rotating member 400 conveniently.

It will be appreciated that the two ends of the second tube may be connected to the mounting plate and the mounting housing by a universal fixing means such as a snap fit means.

Development of the basic embodiment referring to fig. 2-4, the magnetic stirring assembly further comprises:

the driving piece is arranged outside the liquid storage container 100, the driving piece is arranged on the mounting plate 230, the driving piece is particularly arranged at the first end of the mounting plate 230, the first end of the driving shaft 510 penetrates through the mounting plate 230 and is connected with the driving piece, the first end of the driving shaft 510 penetrates through the liquid storage container 100 and the mounting plate 230 and is connected with the driving piece, the driving piece can drive the driving shaft 510 to rotate, the driving piece can adopt a motor, particularly can be a gear motor, the driving piece can be arranged on the outer wall of the liquid storage container 100 in a screw thread mode and other general mounting modes, the driving piece is arranged to drive the driving shaft 510 to rotate outside the liquid storage container 100, and accordingly the first magnetic force rotating piece 400 and the second magnetic force rotating piece 600 are driven to rotate, and the driving piece is arranged outside to facilitate installation.

Further development of the basic embodiment, referring to fig. 2-3, a first end of the driving shaft 510 is provided with a connecting sleeve 520, an output end of the driving member can be connected with the connecting sleeve 520 in a key connection manner, the arrangement of the connecting sleeve 520 is convenient for transmission connection between the driving shaft 510 and the driving member, a second end of the connecting sleeve 520 is fixed at the first end of the driving shaft 510, a groove is arranged at the first end of the connecting sleeve 520, a key slot can be arranged in the groove, an output shaft of the driving member can be inserted into the groove, and a key on the output shaft is matched with the key slot.

It will be appreciated that the drive member and the drive shaft may be connected by other common drive connections.

1-4, a top plate 220 is arranged at a first end of the first tube body 210 of the upper magnetic stirring assembly, the top plate 220 is connected with an inner wall of an upper end of the liquid storage container 100 in a hot melting mode, and the top plate 220 is convenient for fixing the first tube body 210 on the liquid storage container 100 and also convenient for realizing sealing connection between the first end of the first tube body 210 and the inner wall of the upper end of the liquid storage container 100.

It is understood that the upper end wall surface of the liquid storage container is arranged between the top disc of the upper magnetic stirring assembly and the mounting plate of the upper magnetic stirring assembly, and other general sealing connection modes can be adopted between the top disc and the upper end inner wall of the liquid storage container.

The first end of the first pipe body 210 of the lower magnetic stirring assembly is provided with the ventilation disc 110, the ventilation disc 110 is connected with the inner wall of the lower end of the liquid storage container 100 in a hot melting mode, the ventilation disc 110 is convenient to ventilate at the bottom of the liquid storage container 100, and because the ventilation disc 110 is of an original structure on the liquid storage container 100, the ventilation disc 110 can be directly used for replacing a top disc under the requirement that the magnetic stirring assembly is arranged at the lower end of the liquid storage container 100, the magnetic stirring assembly is directly arranged on the ventilation disc 110, the structure is convenient to simplify, the installation of the magnetic stirring assembly is also convenient, and other general sealing connection modes can be adopted between the ventilation disc 110 and the inner wall of the liquid storage container 100.

It is understood that the top disk of the first end of the first tube body is in sealing connection with the inner wall of the liquid storage container, the second end of the first tube body is in sealing connection with the first end of the intermediate connecting tube, the second end of the intermediate connecting tube is in sealing connection with the mounting tube part of the first end of the mounting shell, and the first tube body, the intermediate connecting tube and the mounting shell are matched with each other to isolate the inner cavity of the liquid storage container from the mounting space of the second tube body and the driving shaft.

Development of the basic embodiment referring to fig. 2-4, the first tube 210 is a silicone tube;

the first end of the installation housing 300 includes an installation tube portion 310, the installation tube portion 310 is connected to the second end of the silicone tube, and the second magnetic rotator 600 is sleeved on the installation tube portion 310. The arrangement of the mounting tube portion 310 facilitates the connection of the silicone tube and also facilitates the arrangement of the second magnetic rotator 600.

Further development of the basic embodiment, referring to fig. 2-4, the magnetic stirring assembly further comprises:

the second end of silicone tube is connected to the first end of intermediate connection pipe 910, and installation tube portion 310 is connected to the second end of intermediate connection pipe 910, and the setting of intermediate connection pipe 910 is convenient for sealing connection silicone tube and installation tube portion 310, is convenient for guarantee the leakproofness.

2-4, the first end of the intermediate connecting pipe 910 is provided with an annular convex buckle 920, the annular convex buckle 920 can be buckled into the second end of the silicone tube, and the annular convex buckle 920 is arranged into the second end of the silicone tube in an extrusion manner, so that sealing connection is realized;

the second end of the intermediate connection pipe 910 is screwed to the mounting pipe portion 310 to achieve a sealed connection.

It will be appreciated that the second magnetic rotating member of the upper magnetic stirring assembly is supported by the mounting tube portion of the upper magnetic stirring assembly and the second magnetic rotating member of the lower magnetic stirring assembly is supported by the intermediate connecting tube of the lower magnetic stirring assembly.

Further, the upper magnetic stirring assembly and the lower magnetic stirring assembly are integrally symmetrical; the second magnetic rotating piece of the upper magnetic stirring assembly can be installed and buckled in a groove formed between the installation pipe part and the middle connecting pipe, and the weight of the second magnetic rotating piece of the upper magnetic stirring assembly is supported by the installation pipe part so that the installation of the second magnetic rotating piece is more stable; the second magnetic force rotating piece of the lower magnetic force stirring assembly can be installed and buckled in a groove formed between the installation pipe part and the middle connecting pipe, and the weight of the second magnetic force rotating piece of the lower magnetic force stirring assembly is supported through the middle connecting pipe so that the installation of the second magnetic force rotating piece is firmer.

It is understood that the two ends of the middle connecting pipe can be connected with the silicone pipe and the mounting pipe part by ultrasonic welding, adding a sealing ring and other general sealing connection modes.

It will be appreciated that the radially outer wall of the mounting tube portion may be provided with an annular recess for mounting the second magnetic rotator, or the second magnetic rotator may be mounted by means of a stepped surface on the mounting tube portion cooperating with a space between the second ends of the intermediate connection tubes.

Development of the basic embodiment referring to fig. 2-4, the first magnetic rotator 400 includes:

the first rotating housing is connected with the driving shaft 510, and a threaded hole or a key slot can be arranged at the axial center of the first rotating housing to be matched with the second end of the driving shaft 510;

the first magnetic piece is arranged in the first rotating shell, the inner cavity of the first rotating shell can be annular, and the shape of the first magnetic piece is annular correspondingly. The first rotating housing is capable of protecting the first magnetic member.

It will be appreciated that the first rotary housing may be provided as a two-part housing cap connection facilitating the provision of the first magnetic member internally.

It will be appreciated that the first magnetic rotator may also be formed directly from a magnetic member.

Further development of the basic embodiment, referring to fig. 2-4, the first end of the first rotating housing is connected to the inner wall of the first end of the mounting housing 300 by means of a bearing;

the second end of the first rotating housing is bearing-wise connected to the inner wall of the second end of the mounting housing 300. The bearing type connection enables the first rotating housing to rotate more stably relative to the mounting housing 300, and the two sets of bearings cooperate to enable the first rotating housing not to move in the axial direction during rotation.

It will be appreciated that the outer wall of the first rotating housing and the inner wall of the mounting housing may be provided with stepped surfaces or grooves for bearing mounting.

2-4, the second magnetic rotator 600 includes:

the second rotating shell is sleeved on the mounting shell 300;

the second magnetic part is arranged in the second rotating shell, the inner cavity of the second rotating shell can be annular, the shape of the second magnetic part is annular, the radial section of the second magnetic part can be U-shaped, and the inner wall of the first end of the second rotating shell can be provided with an annular bulge which can be arranged in the second magnetic part. The second rotating housing is capable of protecting the second magnetic member.

It will be appreciated that the second rotary housing may be provided as a two part housing cap connection facilitating the provision of the second magnetic member internally.

It will be appreciated that the second magnetic rotator may also be formed directly from a magnetic member.

It will be appreciated that the magnetic element may be a general purpose magnetic element, and in particular may be a magnet.

Further development of the basic embodiment, referring to fig. 2-4, the second rotating housing is bearing-wise connected to the mounting housing 300. The bearing-wise connection enables the second rotating housing to spin more stably relative to the mounting housing 300.

It will be appreciated that the outer wall of the second rotating housing and the inner wall of the mounting housing may be provided with stepped surfaces or grooves for the mounting of the mating bearings.

2-4, the first end of the drive shaft 510 extends out of the reservoir 100, and the first end of the drive shaft 510 is connected to the outer wall of the reservoir 100 via a bearing, which enables the drive shaft 510 to spin more stably relative to the reservoir 100. A boss may be disposed on a portion of the outer wall of the liquid storage container 100 through which the driving shaft 510 is disposed, a first end of the driving shaft 510 passes through the boss, an annular groove may be disposed at a first end of the boss, a bearing may be disposed in the annular groove, and two ends of the bearing are respectively connected to the driving shaft 510 and an inner sidewall of the annular groove.

The expansion of basic embodiment, the stock solution container is the stock solution bag of deformable, and the stock solution bag can adopt the high separation multilayer that accords with pharmaceutical standard to crowd the membrane heat seal to form, and the folding transportation of being convenient for is also convenient for install, is convenient for regard as disposable bioreactor, has saved the clearance work after once cultivateing the completion, and the mounted position is nimble, and the cost is lower.

Development of the basic embodiment, the bioreactor further comprises:

the liquid inlet pipe group is connected with the first end of the liquid storage container;

the liquid drain pipe group is connected with the second end of the liquid storage container;

the air inlet pipe group is connected with the first end and/or the second end of the liquid storage container;

a gas exhaust pipe group connected with the first end and/or the second end of the liquid storage container;

the sampling tube group is connected with the second end of the liquid storage container;

the bubble generating device is arranged in the liquid storage container and is connected with the air charging port on the liquid storage container.

The liquid inlet pipe group can comprise a feeding pipe group, one end of the feeding pipe group is connected with a feeding port of the liquid storage container, and the other end of the feeding pipe group is connected with an external feeding device; the liquid inlet pipe group can also comprise a material supplementing pipe group, one end of the material supplementing pipe group is connected with a material feeding port of the liquid storage container, and the other end of the material supplementing pipe group is connected with an external material supplementing device. The liquid inlet pipe group is used for inputting materials into the liquid storage container according to the requirements.

One end of the liquid drain pipe group is connected with a liquid drain port of the liquid storage container, and the other end is connected with an external harvesting device. The drain pipe group can be used for draining waste liquid and also can be used for harvesting culture liquid.

The air inlet pipe group and the air exhaust pipe group form an air exhaust pipe group which is used for supplying air or exhausting air into the liquid storage container according to requirements.

One end of the sampling tube group is connected with a sampling port of the liquid storage container, and the other end is connected with an external sampling device. The sampling tube set is used for sampling in the liquid storage container.

The bubble generating device is used for generating bubbles in the liquid storage container.

A sterilizing and filtering device can be arranged in the pipeline of the ventilation pipe group to clean the gas passing through the liquid storage container.

The liquid inlet pipe group, the liquid outlet pipe group, the ventilation pipe group, the sampling pipe group and the like are matched, and an environment for culturing requirements is provided in the cell culturing process.

It is noted that terms like "one embodiment," "some embodiments," "base embodiments," "extended embodiments," and the like may be used throughout this specification to describe several embodiments of the utility model, as a particular feature, structure, material, or characteristic of the several embodiments may be combined without departing from the principles and spirit of the present utility model.

While there has been shown and described what is considered to be certain embodiments of the present utility model, it is to be understood that the utility model is not limited to the above-described embodiments, but is to be accorded the widest scope consistent with the principles and novel features of the present utility model.

Claims (10)

1. The utility model provides a magnetic stirring formula bioreactor from top to bottom which characterized in that includes:

a liquid storage container (100), an upper magnetic stirring assembly and a lower magnetic stirring assembly;

the upper magnetic stirring assembly and the lower magnetic stirring assembly each comprise:

a seal housing assembly;

a first magnetic rotator (400) disposed within the sealed housing assembly;

the second magnetic force rotating piece (600) is arranged outside the sealing shell assembly, and stirring paddles (700) are arranged on the second magnetic force rotating piece (600);

the driving assembly is connected with the first magnetic rotating piece (400) and can drive the first magnetic rotating piece (400) to rotate, and the first magnetic rotating piece (400) can drive the second magnetic rotating piece (600) to rotate by rotating;

the sealing shell component of the upper magnetic stirring component is connected with the inner wall of the upper end of the liquid storage container (100), and the sealing shell component of the lower magnetic stirring component is connected with the inner wall of the lower end of the liquid storage container (100).

2. The up-down magnetic stirring bioreactor of claim 1, wherein the sealed housing assembly comprises:

a first tube (210);

the installation shell (300) is connected with the second end of the first pipe body (210), the first magnetic force rotating piece (400) is arranged in the installation shell (300), and the second magnetic force rotating piece (600) is sleeved on the first pipe body (210) or the installation shell (300);

the first end of the first pipe body (210) of the upper magnetic stirring assembly is connected with the inner wall of the upper end of the liquid storage container (100), and the first end of the first pipe body (210) of the lower magnetic stirring assembly is connected with the inner wall of the lower end of the liquid storage container (100).

3. The up-down magnetic stirring bioreactor as set forth in claim 2, wherein a top plate (220) is disposed at a first end of the first tube body (210) of the upper magnetic stirring assembly, and the top plate (220) is connected to an inner wall of an upper end of the liquid storage container (100) in a hot melting manner;

the first end of the first pipe body (210) of the lower magnetic stirring assembly is provided with an air vent disc (110), and the air vent disc (110) is connected with the inner wall of the lower end of the liquid storage container (100) in a hot melting mode.

4. The up-down magnetic stirring bioreactor of claim 2, wherein the drive assembly comprises:

the middle part of the driving shaft (510) is arranged in the first pipe body (210), and the second end of the driving shaft (510) is inserted into the mounting shell (300) and is connected with the first magnetic force rotating piece (400).

5. The up-down magnetic stirring bioreactor of claim 4, wherein the upper magnetic stirring assembly and the lower magnetic stirring assembly each further comprise:

the middle part of the second pipe body (800) is arranged in the first pipe body (210), and the middle part of the driving shaft (510) is arranged in the second pipe body (800).

6. The up-down magnetic stirring bioreactor of claim 5, wherein the drive assembly further comprises:

the first end of the second pipe body (800) is connected with the mounting plate (230) in a threaded mode, and the second end of the second pipe body (800) is connected with the mounting shell (300) in a threaded mode;

the driving piece is arranged on the mounting plate (230), a first end of the driving shaft (510) penetrates through the mounting plate (230) and is connected with the driving piece, and the driving piece can drive the driving shaft (510) to rotate;

the first end of the driving shaft (510) is provided with a connecting sleeve (520), and the output end of the driving piece is connected with the connecting sleeve (520) in a key connection mode.

7. The up-down magnetic stirring bioreactor as set forth in claim 2, wherein the first tube (210) is a silicone tube;

the first end of the installation shell (300) comprises an installation pipe part (310), and the second magnetic force rotating piece (600) is sleeved on the installation pipe part (310);

the seal housing assembly further includes:

an intermediate connection tube (910), a first end of which is connected to a second end of the silicone tube, the second end of the intermediate connection tube (910) being connected to the mounting tube portion (310);

the second magnetic rotating member (600) of the upper magnetic stirring assembly is supported by the mounting pipe portion of the upper magnetic stirring assembly, and the second magnetic rotating member (600) of the lower magnetic stirring assembly is supported by the intermediate connecting pipe (910) of the lower magnetic stirring assembly.

8. The up-down magnetic stirring bioreactor as set forth in claim 7, wherein a first end of the intermediate connecting tube (910) is provided with an annular male buckle (920), the annular male buckle (920) being capable of buckling into a second end of the silicone tube;

the second end of the intermediate connection tube (910) is connected to the mounting tube portion (310) by means of a screw thread.

9. The up-down magnetic stirring bioreactor as set forth in claim 2, wherein the first magnetic rotator (400) includes:

a first rotary housing provided in the installation housing (300);

the first magnetic piece is arranged in the first rotating shell;

the first end of the first rotating shell is connected with the inner wall of the first end of the mounting shell (300) in a bearing way;

the second end of the first rotating housing is connected with the inner wall of the second end of the mounting housing (300) in a bearing mode.

10. The up-down magnetic stirring bioreactor of claim 2, wherein the second magnetic rotator (600) comprises:

the second rotating shell is sleeved on the mounting shell (300);

the second magnetic piece is arranged in the second rotating shell;

the second rotating housing is connected to the mounting housing (300) via bearings.