CN219272678U - Stem cell derivative filter equipment - Google Patents
Stem cell derivative filter equipment Download PDFInfo
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- CN219272678U CN219272678U CN202320294040.6U CN202320294040U CN219272678U CN 219272678 U CN219272678 U CN 219272678U CN 202320294040 U CN202320294040 U CN 202320294040U CN 219272678 U CN219272678 U CN 219272678U
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Abstract
The utility model discloses a stem cell derivative filtering device, which relates to the technical field of filtering devices and comprises a storage bottle, a feed pump, a buffer bottle, a second filter membrane component and a third filter membrane component which are sequentially connected, wherein the upper end of the storage bottle is provided with a first filter component, the lower end of the buffer bottle is detachably connected with a first connecting piece, the lower end of the first connecting piece is detachably connected with the first filter membrane component, the second filter membrane component is detachably connected with a second connecting piece, the third filter membrane component is detachably connected with a third connecting piece, the first connecting piece, the second connecting piece and the third connecting piece are detachably connected with a fixed bracket, and filter membrane box slots for placing filter membrane boxes are formed in the second filter membrane component and the third filter membrane component. The stem cell derivative realizes primary filtration in the first filter assembly, secondary filtration is realized again in the second filter assembly and the third filter assembly, and the stem cell derivative meets the filtration and purification requirements after three times of filtration.
Description
Technical Field
The utility model relates to the technical field of filter devices, in particular to a stem cell derivative filter device.
Background
As stem cell therapy has come into the general field of view, it brings new therapeutic promise for many rare families, but there are many limitations due to the limited survival time and high cost of stem cells entering the body. On the other hand, since stem cells secrete a variety of growth factors and cytokines during culture, they are involved in inducing angiogenesis, reducing inflammation, promoting cell migration and differentiation, and collagen production and remodeling. Thus, treatment using cell culture supernatants containing these bioactive molecules rather than cell therapy appears to be a safer, cheaper approach. However, how to efficiently and completely isolate stem cells and culture supernatants thereof is a problem to be solved at present. In the existing separation technology, a centrifugal method is generally adopted, and cell fragments, cells and culture supernatant are separated through different centrifugal forces/centrifugal rotation speeds/centrifugal times, but the separation mode has high manual participation, needs to be filled and collected continuously, and has high macromolecular impurity residues.
Chinese patent with publication number CN215939273U and date 2022.03.04 discloses a filtering and separating device for cell supernatant, comprising a pre-loading container, a filtering assembly and a collecting container which are detachably connected in sequence from top to bottom; the filter assembly comprises a hollow block, a conduit is arranged in the hollow block, two ends of the conduit are respectively communicated with the preassembling container and the collecting container so as to form a filter channel, a filter screen capable of placing a filter membrane is arranged at one end of the conduit, an interface is arranged at one side of the hollow block and is communicated with the interior of the hollow block, an air suction piece can be connected to the interface, and an air outlet channel is formed between the interface, the interior of the hollow block and the collecting container. The separation mode can not realize the purpose of one-time separation and purification, and is not beneficial to the mass production of stem cell derivatives (including cell supernatant, secretion groups, cytokines, exosomes and exosomes).
Disclosure of Invention
The utility model aims to solve the problem that the stem cell derivative cannot be separated and purified once, and provides a stem cell derivative filtering device, after stem cells are cultured, the stem cells are stood, non-stem cell liquid sequentially enters a buffer bottle, a second filter membrane assembly and a third filter membrane assembly through a first filter assembly and a feed pump, the stem cell derivative is subjected to primary filtration in the first filter assembly, the second filter membrane assembly and the third filter membrane assembly are subjected to secondary filtration again, and the stem cell derivative meets the filtration and purification requirements after being subjected to tertiary filtration; the buffer bottle, the first connecting piece and the second filter membrane component are sequentially detachably connected, so that the buffer bottle, the second filter membrane component and the first connecting piece can be detached more conveniently, and the first connecting piece, the second connecting piece and the third connecting piece are detachably connected with the fixed support to fix the buffer bottle, the second filter membrane component and the third filter membrane component; the filter membrane is placed in the filter membrane box, and the filter membrane box is placed in second filter membrane subassembly and third filter membrane subassembly, conveniently changes the filter membrane.
The utility model aims at realizing the following technical scheme:
the utility model provides a stem cell derivative filter equipment, includes consecutive storage bottle, charge-in pump, buffer bottle, second filter membrane subassembly and third filter membrane subassembly, storage bottle upper end is provided with first filter component, the buffer bottle lower extreme can be dismantled with first connecting piece and be connected, first connecting piece lower extreme can be dismantled with first filter membrane subassembly and be connected, second filter membrane subassembly can be dismantled with the second connecting piece and be connected, third filter membrane subassembly can be dismantled with the third connecting piece and be connected, first connecting piece, second connecting piece and third connecting piece can all be dismantled with the fixed bolster and be connected, all be provided with the filter membrane box slot that is used for placing the filter membrane box on second filter membrane subassembly and the third filter membrane subassembly.
Preferably, a sealing gasket is arranged around the filter membrane box.
Preferably, a sealing groove matched with the sealing gasket is arranged around the filter membrane box slot.
Preferably, the feed pump is a peristaltic pump.
Preferably, grooves are formed in the lower ends of the storage bottle and the filtrate bottle.
Preferably, the second filter membrane assembly is connected with a third filter membrane assembly through a first drain pipe, and the third filter membrane assembly is connected with a filtrate bottle through a second drain pipe.
Preferably, the air outlets of the first liquid draining pipe fitting and the second liquid draining pipe fitting are connected with a vacuum pump.
Preferably, the lower end of the filtrate bottle is connected with a liquid outlet pipe.
Preferably, the left side and the right side of the first connecting piece, the second connecting piece and the third connecting piece are respectively and fixedly connected with the support in a detachable mode.
Preferably, a pressure sensor and a flowmeter are arranged between the buffer bottle and the feeding pump.
The beneficial effects of this technical scheme are as follows:
1. according to the stem cell derivative filtering device provided by the utility model, after stem cells are cultured, the stem cells are kept stand, the stem cell derivatives sequentially enter the buffer bottle, the second filter membrane assembly and the third filter membrane assembly through the first filter assembly, the stem cell derivatives are subjected to primary filtration in the first filter assembly, the second filter membrane assembly and the third filter membrane assembly are subjected to secondary filtration again, and the stem cell derivatives meet the filtering and purifying requirements after being subjected to tertiary filtration; the buffer bottle, the first connecting piece and the second filter membrane component are sequentially detachably connected, so that the buffer bottle, the second filter membrane component and the first connecting piece can be detached more conveniently, and the first connecting piece, the second connecting piece and the third connecting piece are detachably connected with the fixed support to fix the buffer bottle, the second filter membrane component and the third filter membrane component; the filter membrane is placed in the filter membrane box, and the filter membrane box is placed in second filter membrane subassembly and third filter membrane subassembly, conveniently changes the filter membrane.
2. According to the stem cell derivative filtering device provided by the utility model, the sealing gasket and the sealing groove are arranged, so that the sealing performance of the filter membrane box and the filter membrane box slot is better.
3. The stem cell derivative filtering device provided by the utility model can ensure the micro constant feeding of cell supernatant by using the peristaltic pump.
4. The stem cell derivative filtering device provided by the utility model has the advantages that the arrangement of the grooves reduces the residual of stem cell derivatives and filtrate in the storage bottle and the filtrate bottle.
5. According to the stem cell derivative filtering device provided by the utility model, the left side and the right side of the first connecting piece, the second connecting piece and the third connecting piece are detachably connected through the fixing brackets, so that the buffer bottle, the second filter membrane component and the third filter membrane component are firmer than the fixing brackets.
6. According to the stem cell derivative filtering device provided by the utility model, the pressure sensor is arranged between the buffer bottle and the feed pump, so that the pressure at the rear end of the feed pump can be monitored in real time.
Drawings
FIG. 1 is a schematic diagram of the structure of the present utility model;
FIG. 2 is a schematic diagram of the structure of a first filter membrane module and a second filter membrane module according to the present utility model;
FIG. 3 is a schematic view showing the connection of the first, second and third connectors to the fixing bracket according to the present utility model;
in the figure: 1. a storage bottle; 2. a feed pump; 3. a buffer bottle; 4. a first filter membrane assembly; 5. a second filter membrane assembly; 6. a first connector; 7. a second connector; 8. a fixed bracket; 9. a third connecting member; 10. a vacuum pump; 11. a groove; 12. a first drain pipe; 13. a filtrate bottle; 14. a liquid outlet pipe; 15. a pressure sensor; 16. a filter cartridge; 17. a filter membrane box slot; 18. a sealing gasket; 19. sealing grooves; 20. a second drain pipe fitting; 21. a flow meter; 22. a first filter assembly.
Detailed Description
The present utility model will be described in further detail with reference to examples, but embodiments of the present utility model are not limited thereto.
It is noted that when an element is referred to as being "mounted," "secured," or "disposed" on another element, it can be directly on the other element or intervening elements may also be present. When an element is referred to as being "connected to" another element, it can be directly connected to the other element or intervening elements may also be present.
It should be noted that, in the embodiments of the present utility model, terms such as left, right, up, and down are merely relative concepts or references to normal use states of the product, and should not be construed as limiting.
Example 1
As shown in fig. 1-3, a stem cell derivative filtering device comprises a storage bottle 1, a feed pump 2, a buffer bottle 3, a second filter membrane component 4 and a third filter membrane component 5 which are sequentially connected, wherein a first filter component 22 is arranged at the upper end of the storage bottle 1, the first filter component 22 comprises a filter support and filter paper placed in the filter support, the filter support is hung on the bottle mouth of the storage bottle through a hanging ring, the lower end of the buffer bottle 3 is detachably connected with a first connecting piece 6, the lower end of the first connecting piece 6 is detachably connected with a second filter membrane component 4, first external threads are respectively arranged at the lower end of the buffer bottle 3 and the lower end of the first connecting piece 6, first internal threads matched with the first external threads are respectively arranged at the upper end of the first connecting piece 6 and the upper end of the second filter membrane component 4, the second membrane component 4 is detachably connected with a second connecting piece 7, the lower end of the second filter membrane component 4 is provided with a second external thread, the upper end of the second connecting piece 7 is provided with a second internal thread matched with the second external thread, the third filter membrane component 5 is detachably connected with the third connecting piece 9, the lower end of the third filter membrane component 5 is provided with a third external thread, the upper end of the third connecting piece 9 is provided with a third internal thread matched with the third external thread, the first connecting piece 6, the second connecting piece 7 and the third connecting piece 9 are detachably connected with the fixed bracket 8, the side walls of the first connecting piece 6, the second connecting piece 7 and the third connecting piece 9 are provided with a fourth internal thread, the bracket on the fixed bracket 8 is close to the first connecting piece 6, the second connecting piece 7 and the third connecting piece 9 and is provided with a fourth external thread matched with the fourth internal thread, the second filter membrane component 4 and the third filter membrane component 5 are respectively provided with a filter membrane box slot 17 for placing a filter membrane box 16, the upper end of the filter membrane box 16 is opened, the lower end of the filter membrane box 16 is provided with a plurality of suction holes, the filter membrane box 16 is internally provided with a filter membrane, the filter membrane is a microporous filter membrane, and the filter membrane is made of mixed cellulose, has a diameter of 50mm and a pore diameter of 0.22 mu m. After stem cells are cultured, standing, and sequentially entering a buffer bottle 3, a second filter membrane assembly 4 and a third filter membrane assembly 5 through a first filter assembly 22 and a feed pump 2, wherein the stem cell derivatives realize primary filtration in the first filter assembly 22, and secondary filtration in the second filter membrane assembly 4 and the third filter membrane assembly 5, so that the stem cell derivatives meet the filtration and purification requirements after three times of filtration; the buffer bottle 3, the first connecting piece 6 and the second filter membrane component 4 are sequentially detachably connected, so that the buffer bottle 3, the second filter membrane component 4 and the first connecting piece 6 are more convenient to detach, and the first connecting piece 6, the second connecting piece 7 and the third connecting piece 9 are detachably connected with the fixed bracket 8 to fix the buffer bottle 3, the second filter membrane component 4 and the third filter membrane component 5; the filter membrane is placed in the filter membrane box 16, and the filter membrane box 16 is placed in the second filter membrane component 4 and the third filter membrane component 5, so that the filter membrane can be replaced conveniently.
Example 2
The difference between this embodiment and the embodiment is that: the buffer bottle 3 lower extreme and first connecting piece 6 lower extreme all are provided with first plug connector, first connecting piece 6 upper end and second filter membrane module 4 upper end all are provided with the first slot with first plug connector assorted, second filter membrane module 4 lower extreme is provided with the second plug connector, second connecting piece 7 upper end is provided with the second slot with second plug connector assorted, third filter membrane module 5 lower extreme is provided with the third plug connector, third connecting piece 9 upper end is provided with the third slot with third plug connector assorted, first connecting piece 6, second connecting piece 7 and third connecting piece 9 lateral wall all are provided with the fourth slot, support on the fixed bolster 8 is close to first connecting piece 6, second connecting piece 7 and third connecting piece 9 and holds and is provided with the fourth plug connector with fourth slot assorted.
The periphery of the filter membrane box 16 is provided with a sealing gasket 18, and the periphery of the filter membrane box slot 17 is provided with a sealing groove 19 matched with the sealing gasket 18. The filter membrane box 16 is made of sand core materials, so that the tightness of the filter membrane box 16 and the filter membrane box slot 17 is better.
Wherein, the feeding pump 2 adopts a peristaltic pump, and the use of the peristaltic pump can ensure the trace constant feeding of the stem cell derivative.
Wherein, storage bottle 1 and filtrate bottle 13 lower extreme all set up recess 11, and the setting of recess 11 reduces stem cell derivative and filtrate and remains in storage bottle 1 and filtrate bottle 13.
The second filter membrane component 4 is connected with the third filter membrane component 5 through the first liquid draining pipe fitting 12, the third filter membrane component 5 is connected with the filtrate bottle 13 through the second liquid draining pipe fitting 20, the first liquid draining pipe fitting 12 and the second liquid draining pipe fitting 20 respectively comprise a sleeve outer pipe and a sleeve inner pipe, the sleeve outer pipe and the sleeve inner pipe respectively are connected with the lower end of the second filter membrane component 4 or the lower end of the third filter membrane component 5, the length of the sleeve inner pipe is lower than that of the sleeve outer pipe, an air outlet is formed in the sleeve outer pipe, and the position of the air outlet is higher than that of the sleeve inner pipe.
The air outlets of the first liquid draining pipe fitting 12 and the second liquid draining pipe fitting 20 are connected with the vacuum pump 10, the vacuum pump 10 is connected with the first liquid draining pipe fitting 12 and the second liquid draining pipe fitting 20 through a suction pipe, and a cut-off valve and a pressure regulating valve are arranged on the suction pipe and used for regulating pressure and relieving pressure.
The lower end of the filtrate bottle 13 is connected with a liquid outlet pipe 14, and scales are arranged on the filtrate bottle 13 to detect the amount of filtrate in the filtrate bottle 13; the liquid outlet pipe 14 is provided with a liquid outlet valve, and the liquid outlet valve has the functions of liquid discharging and pressure relief.
Wherein, the left and right sides of the first connecting piece 6, the second connecting piece 7 and the third connecting piece 9 are respectively connected with the fixed brackets 8 in a detachable way. The first connecting piece 6, the second connecting piece 7 and the third connecting piece 9 are detachably connected through the fixing support 8 on the left side and the right side, so that the buffer bottle 3, the second filter membrane assembly 4 and the third filter membrane assembly 5 are firmer than the fixing support 8.
Wherein, be provided with pressure sensor 15 and flowmeter 21 between buffer bottle 3 and the charge pump 2, but real-time supervision charge pump 2 rear end pressure and flow.
Before using, connect buffer bottle 3, first connecting piece 6, second filter membrane subassembly 4, first flowing back pipe fitting 12, third filter membrane subassembly 5, second flowing back pipe fitting 20 and filtrate bottle 13 in proper order, realize with fixed bolster 8's fixed through first connecting piece 6, second connecting piece 7 and third connecting piece 9 afterwards, the stem cell is kept still after cultivateing, afterwards, the stem cell derivative is poured in storage bottle 1, the stem cell derivative realizes once filtering through first filter module 22, start vacuum pump 10 and charge-in pump 2, charge-in pump 2 makes the stem cell derivative flow through buffer bottle 3 in proper order, second filter membrane subassembly 4 and third filter membrane subassembly 5, the stem cell derivative realizes secondary filtration at second filter membrane subassembly 4 and third filter membrane subassembly 5, the stem cell derivative satisfies filtration and purification demand after passing through the tertiary filtration.
The foregoing description is only a preferred embodiment of the present utility model, and is not intended to limit the present utility model in any way, and any simple modification, equivalent variation, etc. of the above embodiment according to the technical matter of the present utility model fall within the scope of the present utility model.
Claims (10)
1. A stem cell derivative filter device, characterized in that: including consecutive storage bottle (1), feed pump (2), buffer bottle (3), second filter membrane subassembly (4) and third filter membrane subassembly (5), storage bottle (1) upper end is provided with first filter component (22), buffer bottle (3) lower extreme can dismantle with first connecting piece (6) and be connected, first connecting piece (6) lower extreme can dismantle with second filter membrane subassembly (4) and be connected, second filter membrane subassembly (4) can dismantle with second connecting piece (7) and be connected, third filter membrane subassembly (5) can dismantle with third connecting piece (9) and be connected, first connecting piece (6), second connecting piece (7) and third connecting piece (9) can dismantle with fixed bolster (8) and be connected, all be provided with on second filter membrane subassembly (4) and third filter membrane subassembly (5) and be used for placing filter membrane box slot (17) of membrane box (16).
2. A stem cell derivative filter device according to claim 1, wherein: and sealing gaskets (18) are arranged around the filter membrane box (16).
3. A stem cell derivative filter device according to claim 2, wherein: and sealing grooves (19) matched with the sealing gaskets (18) are formed in the periphery of the filter membrane box slots (17).
4. A stem cell derivative filter device according to claim 3, wherein: the feeding pump (2) adopts a peristaltic pump.
5. The stem cell derivative filter device of claim 4, wherein: the lower ends of the storage bottle (1) and the filtrate bottle (13) are provided with grooves (11).
6. The stem cell derivative filter device of claim 5, wherein: the second filter membrane component (4) is connected with the third filter membrane component (5) through the first liquid draining pipe fitting (12), and the third filter membrane component (5) is connected with the filtrate bottle (13) through the second liquid draining pipe fitting (20).
7. The stem cell derivative filter device of claim 6, wherein: the air outlets of the first liquid draining pipe fitting (12) and the second liquid draining pipe fitting (20) are connected with the vacuum pump (10).
8. The stem cell derivative filter device of claim 7, wherein: the lower end of the filtrate bottle (13) is connected with a liquid outlet pipe (14).
9. The stem cell derivative filter device of claim 8, wherein: the left side and the right side of the first connecting piece (6), the second connecting piece (7) and the third connecting piece (9) are respectively and fixedly connected with the support (8) in a detachable mode.
10. A stem cell derivative filter device according to claim 1 or 9, wherein: a pressure sensor (15) and a flowmeter (21) are arranged between the buffer bottle (3) and the feeding pump (2).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202320294040.6U CN219272678U (en) | 2023-02-23 | 2023-02-23 | Stem cell derivative filter equipment |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202320294040.6U CN219272678U (en) | 2023-02-23 | 2023-02-23 | Stem cell derivative filter equipment |
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| Publication Number | Publication Date |
|---|---|
| CN219272678U true CN219272678U (en) | 2023-06-30 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202320294040.6U Active CN219272678U (en) | 2023-02-23 | 2023-02-23 | Stem cell derivative filter equipment |
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| Country | Link |
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| CN (1) | CN219272678U (en) |
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- 2023-02-23 CN CN202320294040.6U patent/CN219272678U/en active Active
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