CN219239213U

CN219239213U - Cover removing device of immunoaffinity column

Info

Publication number: CN219239213U
Application number: CN202223182061.3U
Authority: CN
Inventors: 于秋香; 刘凯; 徐同超; 刘红兵
Original assignee: Qingdao Praepang Bioengineering Co ltd
Current assignee: Qingdao Praepang Bioengineering Co ltd
Priority date: 2022-11-30
Filing date: 2022-11-30
Publication date: 2023-06-23
Anticipated expiration: 2032-11-30

Abstract

The application relates to a cap removing device of an immunoaffinity column, which comprises a fixed plate, a clamping mechanism and a driving mechanism; the clamping mechanism comprises a bearing plate, a clamp and a power piece; the driving mechanism is arranged on the fixed plate and used for driving the bearing plate to do reciprocating motion along the vertical direction; the clamp and the power piece are positioned on the bearing plate, the clamp is connected with the power piece, and the power piece is used for driving the clamp to do reciprocating motion along the horizontal direction; the clamp consists of a vertical plate and two clamping plates horizontally and fixedly arranged on the vertical plate; the distance between the two clamping plates is smaller than the diameter of the immunoaffinity column cover plug and larger than the diameter of the immunoaffinity column mouth. The uncapping device can replace manual uncapping, avoids pollution of samples, and can improve working efficiency.

Description

Cover removing device of immunoaffinity column

Technical Field

The application relates to the technical field of biological instruments, in particular to a cap removing device of an immunoaffinity column.

Background

Immunoaffinity columns are commonly referred to as immunoaffinity chromatography columns, often simply referred to as affinity columns, immunoaffinity columns, affinity chromatography columns, and the like; the immunoaffinity column is to make an affinity adsorbent after the antibody is covalently combined with the inert microbeads, then to load the column, then to pass the antigen solution through the immunoaffinity column, and the non-target compound flows down along the column, finally to elute the antigen with an elution buffer solution, thus obtaining the purified antigen. Pure sample extracts can be obtained by utilizing the high specificity of immunoaffinity columns.

Taking aflatoxin extracted from corn as an example, the extraction method comprises the following steps: weighing a crushed sample in a centrifuge tube, adding methanol-water (7:3) for extraction, and carrying out vortex mixing, homogenization, centrifugation and filtration to obtain filtrate; after combining the immunoaffinity column with the upper sample tube, placing the immunoaffinity column on an operation frame, and moving the immunoaffinity column into filtrate to enable a target object (aflatoxin) to be adsorbed on an antibody in a concentrated way; then removing the cover plug of the immunoaffinity column, and washing by using leacheate to remove impurities in the column; finally, after elution by eluent, aflatoxin is obtained.

In practical operation, the removal of the immunoaffinity column plug often requires manual implementation by an operator, which on the one hand easily causes sample pollution and on the other hand also causes the reduction of working efficiency.

Disclosure of Invention

In order to solve the technical problem, the application provides a cap removing device of an immunoaffinity column.

The cover removing device of the immunoaffinity column comprises a fixed plate, a clamping mechanism and a driving mechanism;

the clamping mechanism comprises a bearing plate, a clamp and a power piece;

the driving mechanism is arranged on the fixed plate and used for driving the bearing plate to do reciprocating motion along the vertical direction;

the clamp and the power piece are positioned on the bearing plate, the clamp is connected with the power piece, and the power piece is used for driving the clamp to do reciprocating motion along the horizontal direction;

the clamp consists of a vertical plate and two clamping plates horizontally and fixedly arranged on the vertical plate; the distance between the two clamping plates is smaller than the diameter of the immunoaffinity column cover plug and larger than the diameter of the immunoaffinity column mouth.

By adopting the technical scheme, after the immunoaffinity column and the upper sampling tube are combined, the immunoaffinity column and the upper sampling tube are placed on an operation frame; and then placing the immunoaffinity column on one side of the cap removing device, and moving the position of the immunoaffinity column to enable the immunoaffinity column to be positioned between the two clamping plate horizontal extension lines of the clamp. Starting a driving mechanism to drive the clamp to move upwards, and stopping moving in the vertical direction when the two clamping plates move between the column tube and the cover plug of the immunoaffinity column; then the power piece drives the clamp to move towards the immunoaffinity column, so that a column mouth of the immunoaffinity column is positioned between the two clamping plates, and the cover plug is positioned below the two clamping plates; and then starting the driving mechanism again to drive the clamp to move downwards, so that the cover plug of the immunoaffinity column is pulled down and removed, and the cover removing work is completed. Can replace the manual work to take off the lid through the device that takes off of this application, avoid the pollution of sample to can improve work efficiency.

Optionally, a clamping block is fixedly arranged on the bearing plate, and a chute is arranged on the vertical plate; the vertical plate is connected to the clamping block in a sliding manner along the horizontal direction through the sliding groove.

Optionally, the power piece includes the electro-magnet that moves along the horizontal direction, electro-magnet fixed connection is in the riser.

By adopting the technical scheme, after the electromagnet is electrified, the clamp can be driven to move towards the direction of the immunoaffinity column, so that the immunoaffinity column is positioned between the clamps; after the electromagnet is powered off, the electromagnet can drive the clamp to reset, so that the clamp can move along the horizontal direction.

Optionally, the power piece further comprises a fixed block, the fixed block is fixedly arranged on the bearing plate, and a guide rail is arranged on the fixed block; the electromagnet moves horizontally along the guide rail.

Through adopting above-mentioned technical scheme, the electro-magnet removes along the guide rail on the fixed block, can increase the stability that the electro-magnet removed.

Optionally, the driving mechanism comprises a motor and a screw nut; the bottom of the screw nut is connected to the motor, and the top of the screw nut is connected to the bearing plate.

Through adopting above-mentioned technical scheme, start the motor, the motor passes through screw-nut drive and holds the board and reciprocate to realize the reciprocating of anchor clamps.

Optionally, a connecting plate is arranged on the fixed plate, and a sliding rail is arranged on the connecting plate along the vertical direction; the side wall of the bearing plate is fixedly provided with a sliding block which is connected with the sliding rail in a sliding way.

Through adopting above-mentioned technical scheme, the setting of slider and slide rail can increase the stability that the joint board removed.

Optionally, the cover removing device further comprises a photoelectric sensor and a baffle;

the photoelectric sensor is arranged on the fixed plate and is positioned below the bearing plate;

the baffle is arranged at one end of the bearing plate far away from the connecting plate and is used for being matched with the photoelectric sensor to control the positive and negative rotation of the motor.

Through adopting above-mentioned technical scheme, photoelectric sensor adopts correlation formula photoelectric sensor, and it cooperates with the separation blade, when accepting the board downwardly moving, the separation blade can block photoelectric sensor's light path to realize the output of signal.

In summary, the present application has the following beneficial effects:

the utility model discloses a cooperation through actuating mechanism and anchor clamps can replace the manual work, realizes that mechanical automation takes off the lid, both can avoid the pollution of sample, can improve work efficiency again.

Drawings

Fig. 1 is a schematic overall structure of an embodiment of the present application.

Fig. 2 is a schematic view of the overall structure of the clamping mechanism.

FIG. 3 is a schematic structural diagram of an immunoaffinity column.

In the figure: 1. a fixing plate; 12. a support block; 2. a driving mechanism; 21. a motor; 22. a screw nut; 3. a clamping mechanism; 31. a receiving plate; 311. a clamping block; 312. a slide block; 313. a discharge port; 32. a clamp; 321. a riser; 3211. a chute; 322. a clamping plate; 33. a power member; 331. an electromagnet; 332. a fixed block; 3321. a guide rail; 4. a connecting plate; 41. a slide rail; 5. discharging a hopper; 6. a photoelectric sensor; 61. a transmitting end; 62. a receiving end; 7. a baffle; 8. an immunoaffinity column; 81. a column tube; 82. a pillar mouth; 83. and (5) covering and plugging.

Detailed Description

The present application is further described below with reference to the drawings and examples.

The embodiment of the application discloses a cap removing device of an immunoaffinity column. Referring to fig. 3, the immunoaffinity column 8 includes a column tube 81, a column nozzle 82 and a cover plug 83 from top to bottom.

Referring to fig. 1 and 2, the cover removing device of the immunoaffinity column 8 comprises a fixed plate 1, a driving mechanism 2 arranged on the fixed plate 1, a clamping mechanism 3 connected with the driving mechanism 2, a connecting plate 4 connected with the fixed plate 1 through bolts and a discharging hopper 5 arranged on one side of the clamping mechanism 3.

The driving mechanism 2 includes a motor 21 fixedly connected to the fixing plate 1 by bolts and a screw nut 22 connected to the motor 21. The clamping mechanism 3 comprises a bearing plate 31, a clamp 32 and a power piece 33; the receiving plate 31 is connected to an end of the lead screw nut 22 remote from the motor 21. After the motor 21 is started, the lead screw nut 22 can be driven to move, so that the receiving plate 31 connected to the lead screw nut 22 is driven to reciprocate in the vertical direction.

The clamp 32 is composed of a vertical plate 321 and two clamping plates 322 horizontally and fixedly arranged on the vertical plate 321, and the distance between the two clamping plates 322 is smaller than the diameter of the immunoaffinity column 8 cover plug 83 and larger than the diameter of the immunoaffinity column 8 nozzle 82. The bearing plate 31 is fixedly provided with a clamping block 311, the vertical plate 321 is provided with a sliding groove 3211, and the vertical plate 321 is connected to the clamping block 311 in a sliding manner along the horizontal direction through the sliding groove 3211. The power unit 33 includes an electromagnet 331 and a fixed block 332, the fixed block 332 is fixedly connected to the receiving plate 31 by a bolt, a guide rail 3321 is provided on the fixed block 332, and the electromagnet 331 moves horizontally along the guide rail 3321. One end of the electromagnet 331 is fixedly connected to the vertical plate 321 of the clamp 32, and after the electromagnet 331 is electrified, the electromagnet 331 drives the clamp 32 to move towards the direction close to the connecting plate 4; after the electromagnet 331 is powered off, the electromagnet 331 resets, and drives the clamp 32 to move in a direction away from the connecting plate 4.

The fixing plate 1 is fixedly connected with a Z-shaped supporting block 12 through bolts, and the supporting block 12 is provided with a photoelectric sensor 6 positioned below the bearing plate 31; the photoelectric sensor 6 is a correlation type photoelectric sensor 6 in a U shape and comprises a transmitting end 61 and a receiving end 62; the baffle 7 is fixedly arranged at one end of the receiving plate 31 far away from the connecting plate 4, and the baffle 7 is positioned above the photoelectric sensor 6 and matched with the photoelectric sensor 6 for judging the initial value of the uncapping operation. The side wall bolt connection of the bearing plate 31 near one side of the connecting plate 4 is provided with a U-shaped sliding block 312, the connecting plate 4 is fixedly provided with a sliding rail 41, and the bearing plate 31 is connected with the sliding rail 41 of the connecting plate 4 in a sliding manner along the vertical direction through the sliding block 312. The receiving plate 31 is provided with a discharge hole 313 below the clamp 32, the discharge hopper 5 is obliquely downward and fixedly connected to the receiving plate 31, and the top end of the discharge hopper 5 is communicated with the discharge hole 313.

The working principle of the uncapping device is as follows: after the immunoaffinity column 8 is combined with the upper sample tube, the immunoaffinity column is placed on an operation frame, and then the operation frame is placed on one side of the connecting plate 4 far away from the receiving plate 31; the position of the operating frame is adjusted so that the immunoaffinity column 8 is positioned between the horizontal extension lines of the two clamping plates 322. Before formally uncapping, an initialization test of the uncapping position is performed: starting the motor 21, driving the bearing plate 31 to move upwards by the motor 21 through the screw nut 22, then driving the bearing plate 31 to move downwards by the motor 21 through the screw nut 22, stopping the movement of the bearing plate 31 when the blocking piece 7 starts to block the opposite-type photoelectric sensor 6, and taking the initial value of a cover removing program, namely, a zero point; when the descending distance and the ascending distance of the receiving plate 31 are consistent, the uncapping test is normal.

After the initial value of the uncapping program is determined, the formal uncapping work can be performed. Starting the motor 21, wherein the motor 21 drives the bearing plate 31 to move upwards through the screw nut 22, and stopping moving when the clamping plate 322 is positioned between the column tube 81 and the cover plug 83 of the immunoaffinity column 8; then, the electromagnet 331 is electrified, so that the electromagnet 331 drives the clamp 32 to move towards the direction approaching the connecting plate 4, and the movement is stopped when the nozzle 82 of the immunoaffinity column 8 is positioned between the two clamping plates 322; the motor 21 is started again, the motor 21 drives the receiving plate 31 to move downwards through the screw nut 22, and the clamp 32 moves downwards along with the motor, so that the cover plug 83 of the immunoaffinity column 8 is pulled downwards to be removed, and the removed cover plug 83 passes through a discharge hole 313 on the receiving plate 31 to discharge along the discharge hopper 5. In the process of downward movement of the receiving plate 31, when the blocking piece 7 is inserted between the transmitting end 61 and the receiving end 62 of the photoelectric sensor 6, the light path is blocked, at this time, the downward movement of the receiving plate 31 is stopped, and the motor 21 is controlled to perform forward rotation and reverse rotation through the change of the signal of the photoelectric sensor 6. Through the uncapping device of this application, can realize automatic uncapping to immunoaffinity post 8, improve work efficiency greatly.

The foregoing are all preferred embodiments of the present application, and are not intended to limit the scope of the present application in any way, therefore: all equivalent changes in structure, shape and principle of this application should be covered in the protection scope of this application.

Claims

1. The device for removing the cover of the immunoaffinity column is characterized by comprising a fixed plate (1), a clamping mechanism (3) and a driving mechanism (2);

the clamping mechanism (3) comprises a bearing plate (31), a clamp (32) and a power piece (33);

the driving mechanism (2) is arranged on the fixed plate (1) and used for driving the bearing plate (31) to do reciprocating motion along the vertical direction;

the clamp (32) and the power piece (33) are positioned on the bearing plate (31), the clamp (32) is connected to the power piece (33), and the power piece (33) is used for driving the clamp (32) to reciprocate along the horizontal direction;

the clamp (32) consists of a vertical plate (321) and two clamping plates (322) horizontally and fixedly arranged on the vertical plate (321); the distance between the two clamping plates (322) is smaller than the diameter of the immunoaffinity column (8) cover plug (83) and larger than the diameter of the immunoaffinity column (8) column mouth (82);

the power piece (33) comprises an electromagnet (331) moving along the horizontal direction, and the electromagnet (331) is fixedly connected to the vertical plate (321).

2. The device for removing the cover of the immunoaffinity column according to claim 1, wherein a clamping block (311) is fixedly arranged on the receiving plate (31), and a sliding groove (3211) is arranged on the vertical plate (321); the vertical plate (321) is connected to the clamping block (311) in a sliding manner along the horizontal direction through the sliding groove (3211).

3. The device for removing a cover of an immunoaffinity column according to claim 1, wherein the power piece (33) further comprises a fixed block (332), the fixed block (332) is fixedly arranged on the bearing plate (31), and a guide rail (3321) is arranged on the fixed block (332); the electromagnet (331) moves horizontally along the guide rail (3321).

4. The device for uncapping an immunoaffinity column according to claim 1, wherein the drive mechanism (2) comprises a motor (21) and a screw nut (22); the bottom end of the screw nut (22) is connected to the motor (21), and the top end of the screw nut (22) is connected to the bearing plate (31).

5. The device for removing the cover of the immunoaffinity column according to claim 1, wherein the fixing plate (1) is provided with a connecting plate (4), and the connecting plate (4) is provided with a sliding rail (41) along the vertical direction; a sliding block (312) which is connected with the sliding rail (41) in a sliding way is fixedly arranged on the side wall of the bearing plate (31).

6. The device for uncapping an immunoaffinity column according to claim 2, wherein the uncapping device further comprises a photosensor (6) and a baffle (7);

the photoelectric sensor (6) is arranged on the fixed plate (1) and is positioned below the bearing plate (31);

the baffle (7) is arranged at one end of the bearing plate (31) far away from the connecting plate (4) and is used for being matched with the photoelectric sensor (6) to control the forward and reverse rotation of the motor (21).

7. The device for removing the cover of the immunoaffinity column according to claim 1, wherein the receiving plate (31) is provided with a discharge hole (313) below the clamp (32), the receiving plate (31) is provided with a discharge hopper (5), and the top end of the discharge hopper (5) is communicated with the discharge hole (313).