CN219084760U - Dry chemical reagent strip - Google Patents

Abstract

The utility model belongs to the technical field of medical detection, and discloses a dry chemical reagent strip which sequentially comprises an upper cover body, a sample distribution layer, a filter layer, a sample transfer layer, a reaction test layer and a lower shell from top to bottom. The upper cover body is provided with a sample adding hole; the sample distribution layer comprises a sample distribution groove, a plurality of sample distribution holes and a sample distribution runner, wherein the sample distribution holes are communicated with the sample distribution groove through the sample distribution runner, and the sample distribution groove is opposite to the sample adding holes; the sample transfer layer comprises a transfer groove and a transfer hole which are communicated with each other; the reaction test layer is provided with a plurality of reaction areas, the lower shell is arranged below the reaction test layer, and a plurality of detection holes are formed in the lower shell. The dry chemical reagent strip has the advantages that the whole structure is simple, the production and the manufacture are convenient, the dripped samples can be distributed, the aim of multiple detection of a group of samples is fulfilled, and the filtered blood samples can be transferred, so that the detection holes and the sample adding holes can be arranged in a staggered manner, the influence of residual red blood cells on the filter layer on the detection result can be avoided, and the detection accuracy is improved.

Description

Dry chemical reagent strip

Technical Field

The utility model relates to the technical field of medical detection, in particular to a dry chemical reagent strip.

Background

In clinical test analysis, the results of a plurality of clinical test items can be obtained rapidly by using blood, urine or other body fluids for clinical analysis and test, and the method is one of the most accurate and direct methods for diagnosis by doctors. Dry chemistry is also called solid phase chemistry, which adopts a solid phase reagent technology of multilayer films, only a liquid sample is directly added into a reagent carrier solidified in a special structure, namely, a dry chemical reagent, water in the sample is taken as a solvent, the reagent solidified on the carrier is dissolved and then is subjected to chemical reaction with a component to be detected in the sample, and finally, an optical instrument is utilized to determine the concentration of the detected substance through the intensity of reflected light.

At present, the existing dry chemical reagent strip can realize single-item detection, namely, one-time dropping of a sample can only enter the single-item reaction detection, and a few reagent strips capable of carrying out multi-item reaction detection are mostly complex in structure, and a diffusion layer is often adopted to realize sample adding of a plurality of detection items through capillary phenomenon, such as a dry chemical test strip for simultaneously detecting three blood lipid items in patent CN201721097465, so that the loss of the sample is increased, the stability of sample adding quantity is poor, and the unstable problem of detection results is caused. Moreover, the sample adding hole and the detection hole of the existing dry chemical reagent strip are mostly arranged in the same direction or coaxially, and when the detection hole is used for detecting and observing by using an optical instrument, the residual red blood cells in the dry chemical reagent strip can interfere detection optical signals, so that the accuracy of detection results is affected.

Disclosure of Invention

The utility model aims to provide a dry chemical reagent strip which has high detection accuracy and can realize multiple detection of samples.

To achieve the purpose, the utility model adopts the following technical scheme:

a dry chemical reagent strip comprising, in order from top to bottom:

the upper cover body is provided with a sample adding hole;

the sample distribution layer comprises a sample distribution groove, a plurality of sample distribution holes and a sample distribution runner, wherein the sample distribution holes are communicated with the sample distribution groove through the sample distribution runner, and the sample distribution groove is opposite to the sample adding holes;

a filter layer configured to filter a sample;

the sample transfer layer comprises a transfer groove and a transfer hole which are communicated, and the transfer groove is arranged below the sample distribution hole;

the reaction test layer is arranged below the sample transfer layer, and a plurality of reaction areas are arranged in one-to-one correspondence with the sample distribution holes;

the lower shell is arranged below the reaction test layer and is provided with a plurality of detection holes.

Optionally, the ratio of the length to the width of the sample preparation flow channels is equal.

Optionally, the upper cover, the sample distribution layer, the filter layer, the sample transfer layer, the reaction test layer and the lower housing are adhered and fixed by an adhesive.

Optionally, the adhesive comprises a pressure sensitive adhesive.

Optionally, a plurality of first liquid blocking rings are arranged on the filter layer, the plurality of first liquid blocking rings are opposite to the sample distribution holes one by one, and samples flow into the first liquid blocking rings through the sample distribution holes.

Optionally, the inner wall of the first liquid blocking ring is coated with a hydrophobic layer.

Optionally, the reaction test layer includes the reaction layer and the color development layer of range upon range of, all be provided with a plurality of second liquid blocking ring on reaction layer and the color development layer, a plurality of second liquid blocking ring with a plurality of transfer hole is just right one by one.

Optionally, the inner wall of the second liquid blocking ring is coated with a hydrophobic layer.

Optionally, the upper cover and the lower housing are made of a light-impermeable material.

Optionally, the lower shell is provided with anti-skid stripes.

The utility model has the beneficial effects that:

the dry chemical reagent strip has a simple integral structure, is convenient to produce and manufacture, can distribute dripped samples by the sample distribution layer of the dry chemical reagent strip, so that the samples are distributed into a plurality of groups of small samples, each group of small samples can react with a reaction area of the reaction test layer for color development, and the aim of multi-item detection of one group of samples can be realized. The sample transfer layer of dry chemical reagent strip can shift the blood sample after straining for detection hole and application of sample hole can dislocation set, use optical instrument to detect when observing from the detection hole, can avoid remaining red blood cell on the filter layer to the influence of testing result, do benefit to the accuracy that promotes the detection.

Drawings

FIG. 1 is a schematic diagram of a dry chemical reagent strip according to an embodiment of the present utility model;

fig. 2 is a schematic structural view of a sample distribution layer according to an embodiment of the present utility model.

In the figure:

1. an upper cover; 11. a sample adding hole;

2. a sample distribution layer; 21. a sample preparing hole; 22. a sample preparing groove; 23. sample preparation flow channels;

3. a filter layer; 31. a first liquid blocking ring;

4. a sample transfer layer; 41. a transfer tank; 42. a transfer hole;

5. a reaction test layer; 51. a reaction layer; 52. a color development layer; 53. a second liquid blocking ring;

6. a lower housing; 61. a detection hole; 62. anti-skid stripes.

Detailed Description

Embodiments of the present utility model are described in detail below, examples of which are illustrated in the accompanying drawings, wherein like or similar reference numerals refer to like or similar parts throughout, or parts having like or similar functions. The embodiments described below by referring to the drawings are illustrative and intended to explain the present utility model and should not be construed as limiting the utility model.

In the description of the present utility model, unless explicitly stated and limited otherwise, the terms "connected," "connected," and "fixed" are to be interpreted broadly, as for example, they may be fixedly connected, or may be detachably connected, or may be electrically connected, or may be directly connected, or may be indirectly connected through an intermediary, or may be in communication with one another in two elements or in an interaction relationship between two elements. The specific meaning of the above terms in the present utility model can be understood by those of ordinary skill in the art according to the specific circumstances.

In the description of the present utility model, unless explicitly stated and limited otherwise, a first feature "above" or "below" a second feature may include the first feature and the second feature being in direct contact, or may include the first feature and the second feature not being in direct contact but being in contact by another feature therebetween. Moreover, a first feature being "above," "over" and "on" a second feature includes the first feature being directly above and obliquely above the second feature, or simply indicating that the first feature is higher in level than the second feature. The first feature being "under", "below" and "beneath" the second feature includes the first feature being directly under and obliquely below the second feature, or simply means that the first feature is less level than the second feature.

In the description of the present embodiment, the terms "upper", "lower", "left", "right", and the like are orientation or positional relationships based on those shown in the drawings, merely for convenience of description and simplicity of operation, and do not indicate or imply that the apparatus or elements referred to must have a specific orientation, be constructed and operated in a specific orientation, and thus should not be construed as limiting the utility model. Furthermore, the terms "first," "second," and the like, are used merely for distinguishing between descriptions and not for distinguishing between them.

The technical scheme of the utility model is further described below by the specific embodiments with reference to the accompanying drawings.

The utility model provides a dry chemical reagent strip, which sequentially comprises an upper cover body 1, a sample distribution layer 2, a filter layer 3, a sample transfer layer 4, a reaction test layer 5 and a lower shell 6 from top to bottom as shown in fig. 1. The upper cover body 1 is provided with a sample adding hole 11. The sample distribution layer 2 comprises a sample distribution groove 22, a plurality of sample distribution holes 21 and sample distribution flow channels 23, wherein the sample distribution holes 21 are communicated with the sample distribution groove 22 through the sample distribution flow channels 23, and the sample distribution groove 22 is opposite to the sample adding holes 11. The filter layer 3 is configured to filter the sample. The sample transfer layer 4 includes a transfer well 41 and a transfer hole 42 which are communicated, and the transfer well 41 is placed under the sample preparation hole 21. The reaction test layer is arranged below the sample transfer layer 4, a plurality of reaction areas are arranged on the reaction test layer 5 in one-to-one correspondence with the sample distribution holes 21, the lower shell 6 is arranged below the reaction test layer 5, and a plurality of detection holes 61 are arranged on the lower shell 6.

Specifically, as shown in fig. 1, the upper cover 1 is provided with a circular sample loading hole 11, and the sample distribution layer 2 includes a sample distribution groove 22, a plurality of sample distribution holes 21 and a sample distribution flow channel 23. In this embodiment, four groups of sample preparing holes 21 and sample preparing channels 23 are provided, the sample preparing holes 21 are in a through hole structure, the sample preparing grooves 22 are in a round groove structure with a closed bottom surface, the sample preparing holes 21 and the sample preparing grooves 22 are communicated through the sample preparing channels 23, the sample preparing grooves 22 are opposite to the sample adding holes 11, blood samples are dripped into the sample preparing grooves 22 through the sample adding holes 11, and due to capillary action, the blood samples can flow in the direction of the sample preparing holes 21 after being divided into four parts through the sample preparing channels 23, and the sample distributing layer 2 is in a microfluidic structure and cannot lose samples due to sample diffusion. The filter layer 3 is a porous material membrane, specifically, a blood filter membrane, such as a glass fiber blood filter membrane, a polyester fiber blood filter membrane, and the like, and can filter substances in the blood sample, such as cells, which are easy to interfere with the detection result, and filter red blood cells in the blood sample. The filtered blood sample further falls into the sample transfer layer 4, the sample transfer layer 4 is made of hydrophilic and liquid-impermeable materials, a transfer groove 41 and a transfer hole 42 which are communicated are formed in the sample transfer layer 4, the transfer groove 41 is of a round groove structure with a closed bottom, the transfer hole 42 is of a through hole structure, the transfer groove 41 and the transfer hole 42 are communicated, the filtered blood sample drops into the transfer groove 41, drops into the reaction test layer 5 below the transfer hole 42, a plurality of reaction areas are formed in the reaction test layer 5, the reaction areas are specifically areas coated with various reaction reagents, the reaction test layer 5 is coated with various reaction reagents, the reaction reagents can chemically react with the filtered blood sample and develop colors, for example, in the application, four different reaction reagents can be coated on the reaction test layer 5, namely, the reaction reagents below each transfer hole 42 are different, the effect of multiple detection can be achieved simultaneously, after the sample and the reaction reagents are fully reflected, the reaction color development of the reaction instrument is observed through the detection holes 61 on the lower shell 6 by using an optical instrument.

The sample distribution layer 2 in this embodiment can distribute the dropped samples, so that the samples are distributed into multiple groups of small samples, each group of small samples can react with the reaction test layer to develop color, multiple detection purposes of one group of samples can be achieved, furthermore, the sample transfer layer 4 can transfer the filtered blood samples, so that the detection holes 61 and the sample adding holes 11 can be arranged in a staggered manner, and when the optical instrument is used for detecting and observing from the detection holes 61, the influence of residual red blood cells on the filter layer 3 on the detection result can be avoided, the interference of the red blood cells on detection optical signals is eliminated, and the detection accuracy is improved.

Preferably, in this embodiment, the blood sample can be equally divided into multiple groups of small samples by the sample distribution layer 2, as shown in fig. 2, in this embodiment, four sample distribution holes 21 are provided on the sample distribution layer 2, and sample distribution grooves 22 are provided at middle positions of the sample distribution holes B and the sample distribution holes C, so that the volumes of the samples flowing into the sample distribution holes 21 by the sample distribution grooves 22 are equal, and when the sample distribution channels 23 are designed, the flow resistance coefficients a of the sample distribution channels 23 are equal, that is, the ratio of the lengths and the widths of the sample distribution channels 23 is equal, and in this embodiment, the lengths of the sample distribution channels 23 of the sample distribution holes a and the sample distribution holes D are 6mm, and the widths of the sample distribution channels 23 are 2mm; the length of the sample preparation flow channel 23 of the sample preparation hole B and the sample preparation hole C is 3mm, the width is 1mm, and the resistance coefficient a of the sample preparation hole A, B, C, D is 3, so that the blood sample in the sample preparation groove 22 can equally flow into the sample preparation hole A, B, C, D, and the detection accuracy is improved.

Preferably, as shown in fig. 1, a plurality of first liquid blocking rings 31 are formed on the filtering layer 3 in this embodiment, the plurality of first liquid blocking rings 31 are opposite to the plurality of sample preparing holes 21 one by one, the sample flows into the first liquid blocking rings 31 through the sample preparing holes 21, the first liquid blocking rings 31 can resist the dripped blood sample, prevent the blood sample from diffusing to the outer edge, and reduce the residual quantity of the sample on the filtering layer 3. Preventing inaccurate detection caused by less blood sample. Further, the inner wall of the first liquid blocking ring 31 can be optionally coated with a hydrophobic layer, so that the diffusion range of the sample can be further effectively controlled, and the residual quantity of the sample can be reduced.

Alternatively, as shown in fig. 1, the reaction test layer 5 of the present embodiment includes a laminated reaction layer 51 and a color development layer 52, various kinds of reaction reagents are coated on the reaction layer 51, various kinds of color development reagents are coated on the color development layer 52, the filtered blood sample is dripped onto the reaction layer 51 through the transfer hole 42, and after chemical reaction with the corresponding reaction reagent on the reaction layer 51, the blood sample falls onto the color development layer 52, and further color development reaction with the color development reagent occurs on the color development layer 52. A plurality of second liquid blocking rings 53 are arranged on the reaction layer 51 and the color developing layer 52, the second liquid blocking rings 53 are opposite to the transfer holes 42 one by one, and the second liquid blocking rings 53 and the reaction layer 51 or the color developing layer 52 are integrally formed and play a role in preventing sample diffusion in the same way as the first liquid blocking rings 31. Further, the inner wall of the second liquid blocking ring 53 is coated with a hydrophobic layer, which is favorable for further effectively controlling the diffusion range, reducing the residual quantity, solving the problem of crosstalk between the second liquid blocking rings 53 and improving the detection accuracy. In addition, the reaction layer 51 and the color development layer 52 may be laminated as one layer, including but not limited to the structure shown in the drawings of the present embodiment.

Optionally, the upper cover 1 and the lower housing 6 are made of a light-impermeable material, in particular a black plastic piece, so as to reduce the influence of ambient light on the detection result. Further, the lower casing 6 may further be provided with anti-slip strips 62, which is beneficial to hand holding and prevents easy slipping.

Optionally, the upper cover 1, the sample distribution layer 2, the filter layer 3, the sample transfer layer 4, the reaction test layer 5 and the lower casing 6 in this embodiment are adhered and fixed by an adhesive, so that a complex connection structure design is avoided, the cost is low, and the connection speed is high. Specifically, the adhesive piece can be selected from pressure-sensitive adhesive, all layers of the dry chemical reagent strip are overlapped together in sequence in multiple layers, all layers are tightly adhered together by the pressure-sensitive adhesive, rapid adhesion of all layers is realized, and batch production is facilitated. Moreover, since the number of dry reagent strips in this embodiment is large, in actual production and processing, the upper cover 1, the sample distribution layer 2, the sample transfer layer 4 and the lower housing 6 may be produced and manufactured by injection molding, and a plurality of parts may be produced and manufactured in batch at a time, and weak connection is adopted between the parts, and later separated by cutting. The filtering layer 3, the reaction layer 51 and the color developing layer 52 are printed by a semiconductor printing technology and are perforated at specific positions, so that later gluing is facilitated.

It is to be understood that the above examples of the present utility model are provided for clarity of illustration only and are not limiting of the embodiments of the present utility model. Other variations or modifications of the above teachings will be apparent to those of ordinary skill in the art. It is not necessary here nor is it exhaustive of all embodiments. Any modification, equivalent replacement, improvement, etc. which come within the spirit and principles of the utility model are desired to be protected by the following claims.

Claims (10)

1. A dry chemical reagent strip comprising, in order from top to bottom:

the upper cover body (1), the upper cover body (1) is provided with a sample adding hole (11);

the sample distribution layer (2), the sample distribution layer (2) comprises a sample distribution groove (22), a plurality of sample distribution holes (21) and a sample distribution runner (23), the sample distribution holes (21) are communicated with the sample distribution groove (22) through the sample distribution runner (23), and the sample distribution groove (22) is opposite to the sample adding hole (11);

a filter layer (3) configured to filter a sample;

a sample transfer layer (4), wherein the sample transfer layer (4) comprises a transfer groove (41) and a transfer hole (42) which are communicated, and the transfer groove (41) is arranged below the sample preparation hole (21);

the reaction test layers (5) are arranged below the sample transfer layer (4), and a plurality of reaction areas are arranged in one-to-one correspondence between the reaction test layers (5) and the sample distribution holes (21);

and a lower shell (6) arranged below the reaction test layer (5), wherein a plurality of detection holes (61) are formed in the lower shell (6).

2. Dry chemical reagent strip according to claim 1, characterized in that the ratio of the length to the width of a plurality of sample application channels (23) is equal.

3. The dry reagent strip according to claim 1, wherein the upper cover (1), the sample distribution layer (2), the filter layer (3), the sample transfer layer (4), the reaction test layer (5) and the lower housing (6) are bonded and fixed by an adhesive.

4. A dry reagent strip as claimed in claim 3 wherein the adhesive comprises a pressure sensitive adhesive.

5. The dry chemical reagent strip according to claim 1, wherein the filter layer (3) is provided with a plurality of first liquid blocking rings (31), the plurality of first liquid blocking rings (31) are opposite to the plurality of sample preparing holes (21), and a sample flows into the first liquid blocking rings (31) through the sample preparing holes (21).

6. The dry reagent strip according to claim 5, wherein the inner wall of the first liquid barrier ring (31) is coated with a hydrophobic layer.

7. The dry chemical reagent strip according to claim 1, wherein the reaction test layer (5) comprises a laminated reaction layer (51) and a color development layer (52), a plurality of second liquid blocking rings (53) are arranged on the reaction layer (51) and the color development layer (52), and the second liquid blocking rings (53) are opposite to the transfer holes (42) one by one.

8. The dry reagent strip of claim 7, wherein the inner wall of the second liquid barrier ring (53) is coated with a hydrophobic layer.

9. Dry reagent strip according to any of claims 1-8, characterized in that the upper cover (1) and the lower housing (6) are made of a light-tight material.

10. Dry reagent strip according to any of claims 1-8, characterized in that the lower housing (6) is provided with anti-slip strips (62).

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