CN219058997U - Small storage clamp for cell culture bag - Google Patents
Small storage clamp for cell culture bag Download PDFInfo
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- CN219058997U CN219058997U CN202223313179.5U CN202223313179U CN219058997U CN 219058997 U CN219058997 U CN 219058997U CN 202223313179 U CN202223313179 U CN 202223313179U CN 219058997 U CN219058997 U CN 219058997U
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- cell culture
- culture bag
- fixing plate
- rotating shaft
- small storage
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Abstract
The utility model discloses a small storage clamp for a cell culture bag, which comprises the cell culture bag and further comprises a fixing plate with a U-shaped cross section, wherein notches are formed in the end parts of the fixing plate, a rotating shaft is arranged in the notches, the rotating shaft penetrates through the fixing plate and rotates relative to the fixing plate, a cylinder fixedly connected with the rotating shaft is arranged on the rotating shaft, elastic layers abutted against the cylinder are arranged on the periphery of the cylinder, different elastic layers are abutted against each other, a clamping structure is arranged on the fixing plate and used for clamping the fixing plate and the cell culture bag together, and the device can realize the adjustment of the size of a closed area of the cell culture bag, is suitable for cell culture work of different scales and ensures the cell culture effect.
Description
Technical Field
The utility model relates to the field of cell culture, in particular to a small storage clamp for a cell culture bag.
Background
Suspension cells are cells that grow in suspension in a culture medium, such as lymphocytes, independent of the surface of the support. In the conventional suspension cell culture process, fresh culture medium needs to be periodically supplemented to maintain a culture environment suitable for cell growth. The culture containers are sequentially used according to the normal use sequence of the process: multi-well plates (e.g., 96-well plates, 24-well plates, 6-well plates), culture flasks (e.g., T25 flask, T75 flask, T175 flask), cell culture bags (e.g., 1L culture bag, 2L culture bag, 3L culture bag, 10L culture bag, etc.). Most of the culture staff use a culture flask in the initial stage of culture, and the open culture increases the probability of cell pollution and the culture cost. In view of this, culture bags are used in the early stage of technological culture and in the whole culture process, and are generally prepared from materials with waterproof and breathable films, so that the risk of cell pollution can be reduced and the culture cost can be reduced by using the culture bags. However, the volume of the cell culture bag is a fixed size, and in the cell culture process, not only is the density of the cell suspension required, but also the actual contact area of the cell culture bag is required.
Disclosure of Invention
The small storage clamp for the cell culture bag aims at solving the technical problems that the size of a closed area of the cell culture bag can be adjusted, the small storage clamp is applicable to cell culture work of different scales, and the cell culture effect is guaranteed.
The utility model provides a small storage clamp for a cell culture bag, which comprises the cell culture bag and further comprises fixing plates with U-shaped cross sections, wherein notches are formed in the end parts of the fixing plates, rotating shafts are arranged in the notches, pass through the fixing plates, rotate relative to the fixing plates, are provided with cylinders fixedly connected with the rotating shafts, elastic layers abutted against the cylinders are arranged on the peripheries of the cylinders, different elastic layers are abutted against each other, and clamping structures are arranged on the fixing plates and used for clamping the fixing plates and the cell culture bag together.
Preferably, the clamping structure comprises a fixing rod, the fixing rods fixedly connected with the fixing plate are arranged on two sides of the fixing plate, a U-shaped piece is arranged on the fixing rod, the fixing rod penetrates through the end portion of the U-shaped piece, the U-shaped piece rotates relative to the fixing rod, a clamping groove is formed in the inner wall of the fixing plate, and the U-shaped piece is used for clamping the cell culture bag into the clamping groove.
Preferably, one end of the rotating shaft is provided with a fixed block, and the other end of the rotating shaft is provided with a movable block detachably connected with the fixed block.
Preferably, an external thread is arranged at one end of the rotating shaft, a thread groove is arranged on the movable block, and the rotating shaft is in threaded connection with the thread groove through the external thread.
Preferably, a through hole is formed in the end portion of the U-shaped piece, and the fixing rod penetrates through the through hole.
Preferably, the surface of the U-shaped piece is provided with a plurality of protruding parts.
The utility model has the beneficial effects that:
according to the small storage clamp for the cell culture bag, one end of the cell culture bag to be used is inserted between the elastic layers on different cylinders, then the cell culture bag is pulled towards the direction of the fixing plate, or the cylinders are pushed to move towards the direction of the cell culture bag, namely, opposite movement between the cell culture bag and the fixing plate is achieved until the cylinders are close to the inlet of the cell culture bag, so that the first step is completed, then the inlet of the cell culture bag is continuously filled with cell culture liquid with a required volume, then the cell culture bag or the fixing plate is pulled, so that the cell culture bag and the fixing plate are reversely moved, the closed culture space of the cell culture bag is stably increased, after the cell culture bag is increased to the required volume, the fixing plate and the cell culture bag are combined together through the clamping structure, so that relative movement between the cell culture bag and the fixing plate is avoided, further, the volume of the cell culture bag is enabled to be further avoided, then the whole culture bag is not to be easily replaced, the whole culture structure is further required, the cell culture bag is further required to be replaced, the air-permeable, the cell culture bag is further required to be fully replaced, and the cell culture device is further required to be used, the whole culture structure is further required to be replaced, the cell culture is further is convenient, the cell culture is air-permeable, the whole culture bag is required to be completely, and the cell culture bag is replaced, and the cell culture structure is completely is required to be completely, and the cell culture is in the cell culture bag is in a cell culture bag, and the cell culture bag is in a cell culture bag, and a cell culture bag.
Drawings
In order to more clearly illustrate the technical solutions of the embodiments of the present utility model, the drawings required for the description of the embodiments will be briefly described below, it being obvious that the drawings in the following description are only preferred embodiments of the present utility model, and that other drawings may be obtained according to these drawings without inventive effort for a person skilled in the art.
FIG. 1 is a perspective view of a first embodiment of a compact receiving clip for a cell culture bag of the present utility model;
FIG. 2 is a perspective view of a second embodiment of a compact receiving clip for a cell culture bag according to the present utility model;
FIG. 3 is a top view of a second embodiment of a compact receiving clip for a cell culture bag according to the utility model;
FIG. 4 is an enlarged partial schematic view of FIG. 3A of a second embodiment of a small storage clip for a cell culture bag according to the utility model.
In the figure, 1 is a cell culture bag, 2 is a fixed plate, 3 is a notch, 4 is a rotating shaft, 5 is a cylinder, 6 is an elastic layer, 7 is a fixed rod, 8 is a U-shaped piece, 9 is a clamping groove, 10 is a fixed block, 11 is a movable block, 12 is an external thread, 13 is a thread groove, 14 is a through hole, and 15 is a protruding part.
Detailed Description
For a better understanding of the technical content of the present utility model, specific examples are provided below and the present utility model is further described with reference to the accompanying drawings.
Example 1
Referring to fig. 1, the utility model provides a small storage clamp for a cell culture bag, comprising a cell culture bag 1, and further comprising a fixing plate 2 with a U-shaped cross section, wherein the end part of the fixing plate 2 is provided with a notch 3, a rotating shaft 4 is arranged in the notch 3, the rotating shaft 4 penetrates through the fixing plate 2, the rotating shaft 4 rotates relative to the fixing plate 2, a cylinder 5 fixedly connected with the rotating shaft 4 is arranged on the rotating shaft 4, an elastic layer 6 abutted with the periphery of the cylinder 5 is arranged on the periphery of the cylinder, different elastic layers 6 are abutted with each other, a clamping structure is arranged on the fixing plate 2, the clamping structure is used for clamping the fixing plate 2 and the cell culture bag 1 together, one end of the cell culture bag 1 to be used is inserted between the elastic layers 6 on different cylinders 5, then the cell culture bag 1 is pulled towards the direction of the fixing plate 2, or pushing the cylinder 5 to move towards the cell culture bag 1, namely, realizing opposite movement between the cell culture bag 1 and the fixed plate 2 until the cylinder 5 approaches to the inlet of the cell culture bag 1, thereby completing the first step, then continuously filling the inlet of the cell culture bag 1 with a required volume of cell culture solution, then pulling the cell culture bag 1 or the fixed plate 2, so that the cell culture bag 1 and the fixed plate 2 move reversely, thereby stably increasing the closed culture space of the cell culture bag 1, after the cell culture bag 1 is increased to the required volume, the fixed plate 2 and the cell culture bag 1 can be clamped together through the clamping structure, thereby avoiding the relative movement between the cell culture bag 1 and the fixed plate 2, and then make the volume of the sealed culture space of cell culture bag 1 stable, do benefit to cell culture to accomplish the second step, when needs further enlarge the cultivation scale, only need with the chucking structure is packed up, makes cell culture bag 1 with fixed plate 2 no longer is in the same place, then repeat the operation of second step can, because cell culture bag 1 can ventilate, guarantees cell culture bag 1 is interior cultivate cell suspension can fully exchange with gas, does benefit to the cultivation of cell, can adjust the cultivation scale according to actual need simultaneously, and the suitability is stronger, and this device overall structure is simple moreover, easily the going on of follow-up clean disinfection work.
Specifically, the chucking structure includes dead lever 7, the both sides of fixed plate 2 all are equipped with its fixed connection dead lever 7, be equipped with U-shaped piece 8 on the dead lever 7, dead lever 7 passes the tip of U-shaped piece 8, U-shaped piece 8 is relative dead lever 7 rotates, the inner wall of fixed plate 2 is equipped with draw-in groove 9, U-shaped piece 8 be used for with cell culture bag 1 card is gone into in draw-in groove 9, for avoiding cell culture bag 1 appears rocking and leads to cell culture bag 1's culture space grow, influences the area of contact of cell suspension and air, and then easily appears the relatively poor problem of cell culture effect, will cell culture bag 1 with when fixed plate 2 is in the same place, can compress tightly and will cell culture bag 1 card is gone into draw-in groove 9, thereby avoid cell culture bag 1 can move about at will, but also can be through with the cooperation each other between the elastic layer 6 in order to realize keeping cell culture bag 1's space is big stable.
Example two
Referring to fig. 2 to 4, the difference between the present embodiment and the first embodiment is that a fixed block 10 is disposed at one end of the rotating shaft 4, a movable block 11 detachably connected to the other end of the rotating shaft 4 is disposed at the other end of the rotating shaft, and when the elastic layer 6 loses elasticity or is partially recessed irreversibly due to long-term use, the elastic layer 6 can be replaced conveniently by detaching the movable block 11, and the operation is simple and convenient.
Specifically, an external thread 12 is arranged at one end of the rotating shaft 4, a thread groove 13 is arranged on the movable block 11, the rotating shaft 4 is in threaded connection with the thread groove 13 through the external thread 12, and the movable block 11 is convenient to detach from the rotating shaft 4 through the connection between the external thread 12 on the rotating shaft 4 and the thread groove 13.
Specifically, the end of the U-shaped member 8 is provided with a through hole 14, the fixing rod 7 passes through the through hole 14, and the U-shaped member 8 can be conveniently rotated by matching the through hole 14 on the U-shaped member 8 with the fixing rod 7, so that the U-shaped member 8 can be lifted off and pressed into the clamping groove 9 according to actual needs.
Specifically, the surface of the U-shaped member 8 is provided with a plurality of protruding portions 15, and the protruding portions 15 may be made of an elastic material, so that the U-shaped member 8 can further compress the cell culture bag 1 and the clamping groove 9 under the action of the protruding portions 15, and friction force between the cell culture bag 1 and the clamping groove 9 is increased, so as to avoid separation between the cell culture bag 1 and the clamping groove 9.
The foregoing description of the preferred embodiments of the utility model is not intended to be limiting, but rather is intended to cover all modifications, equivalents, alternatives, and improvements that fall within the spirit and scope of the utility model.
Claims (6)
1. The small storage clamp for the cell culture bag comprises a cell culture bag and is characterized by further comprising a fixing plate with a U-shaped cross section, wherein the end part of the fixing plate is provided with a notch, a rotating shaft is arranged in the notch, the rotating shaft penetrates through the fixing plate, the rotating shaft rotates relative to the fixing plate, a cylinder fixedly connected with the rotating shaft is arranged on the rotating shaft, an elastic layer abutted against the cylinder is arranged on the periphery of the cylinder, different elastic layers are abutted against each other, and a clamping structure is arranged on the fixing plate and used for clamping the fixing plate and the cell culture bag together.
2. The small storage clamp for a cell culture bag according to claim 1, wherein the clamping structure comprises fixing rods, the fixing rods fixedly connected with the fixing rods are arranged on two sides of the fixing plate, U-shaped pieces are arranged on the fixing rods, the fixing rods penetrate through the end parts of the U-shaped pieces, the U-shaped pieces rotate relative to the fixing rods, clamping grooves are formed in the inner walls of the fixing plate, and the U-shaped pieces are used for clamping the cell culture bag into the clamping grooves.
3. The small-sized receiving clip for a cell culture bag according to claim 1, wherein one end of the rotation shaft is provided with a fixed block, and the other end of the rotation shaft is provided with a movable block detachably connected thereto.
4. A small storage clamp for a cell culture bag according to claim 3, wherein one end of the rotating shaft is provided with an external thread, the movable block is provided with a thread groove, and the rotating shaft is in threaded connection with the thread groove through the external thread.
5. The small storage clip for a cell culture bag of claim 2, wherein the end of the U-shaped member is provided with a through hole through which the fixing rod passes.
6. The small storage clip for cell culture bags of claim 2, wherein the surface of the U-shaped member is provided with a plurality of protrusions.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN202223313179.5U CN219058997U (en) | 2022-12-11 | 2022-12-11 | Small storage clamp for cell culture bag |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN202223313179.5U CN219058997U (en) | 2022-12-11 | 2022-12-11 | Small storage clamp for cell culture bag |
Publications (1)
Publication Number | Publication Date |
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CN219058997U true CN219058997U (en) | 2023-05-23 |
Family
ID=86346728
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
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CN202223313179.5U Active CN219058997U (en) | 2022-12-11 | 2022-12-11 | Small storage clamp for cell culture bag |
Country Status (1)
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CN (1) | CN219058997U (en) |
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2022
- 2022-12-11 CN CN202223313179.5U patent/CN219058997U/en active Active
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