CN219016675U

CN219016675U - Laser pulse dispersion compensating device

Info

Publication number: CN219016675U
Application number: CN202222502662.1U
Authority: CN
Inventors: 李敏; 唐星; 王斯博; 王艳; 周镇乔; 吕晶; 贾宏博; 陈月岩
Original assignee: Suzhou Institute of Biomedical Engineering and Technology of CAS
Current assignee: Suzhou Institute of Biomedical Engineering and Technology of CAS
Priority date: 2022-09-21
Filing date: 2022-09-21
Publication date: 2023-05-12
Anticipated expiration: 2032-09-21

Abstract

The utility model discloses a laser pulse dispersion compensation device, which belongs to the field of two-photon microscopic imaging instruments, wherein a first adjusting device drives a first movable reflector to rotate relative to a bracket so as to adjust the angle of the first movable reflector relative to the bracket, a second adjusting device drives a second movable reflector to rotate and/or move relative to the bracket so as to adjust the position and/or angle of the second movable reflector relative to the bracket, and a movable reflector component is positioned between two dispersion compensation components.

Description

Laser pulse dispersion compensating device

Technical Field

The utility model relates to the field of two-photon microscopic imaging instruments, in particular to a laser pulse dispersion compensation device.

Background

Two-photon microscopy is of great importance in the field of biology, especially in the study of neurobiology. The two-photon microscope is an indispensable tool in three-dimensional imaging of living organism tissues, and is one of the best instruments for researching the active signals of the living organism. The introduced material dispersion causes the femtosecond laser to generate serious pulse broadening, and the laser power density in unit volume of unit time is rapidly reduced along with the serious pulse broadening, whether the optical elements are used in a large amount in a conventional two-photon microscope or the optical fibers in a miniature two-photon microscope. Since the excitation efficiency of two-photon fluorescence is proportional to the square of the instantaneous power of the pulse laser, and further, the fluorescence excitation efficiency is greatly reduced due to the broadening of the femtosecond laser pulse caused by material dispersion, dispersion compensation of the femtosecond laser is essential for obtaining high-quality fluorescence imaging.

In order to adapt to different application scenes and experimental conditions, optical elements used by each two-photon microscopic imaging system are not identical, the dispersion quantity generated in the imaging system is not a fixed value, and the stretching degree of the femtosecond laser pulse changes with the system. However, there are certain limitations to the dispersion compensation units used in most two-photon microscopy imaging systems in the market: (1) The compensation amount of a dispersion compensation unit used in the femtosecond two-photon microscopic imaging system is fixed and cannot be adjusted; (2) unique usage scenario, lack of flexibility.

Disclosure of Invention

In order to overcome the defects in the prior art, one of the purposes of the utility model is to provide a laser pulse dispersion compensation device capable of changing the transmission path of laser in a dispersion compensation structure and adjusting the dispersion amount of laser pulse compensation.

One of the purposes of the utility model is realized by adopting the following technical scheme:

the utility model provides a laser pulse dispersion compensation arrangement, includes support, laser alignment structure, dispersion compensation structure, first adjusting device and second adjusting device, the laser alignment structure is including the incidence aligner that sets gradually, movable mirror subassembly, fixed mirror subassembly and exit alignment ware, the movable mirror subassembly includes first movable mirror and second movable mirror, the fixed mirror subassembly includes two fixed mirror units, incidence aligner, exit alignment ware and two fixed mirror units are fixed in respectively the support, first movable mirror installs in first adjusting device, first adjusting device drives first movable mirror rotates with respect to the support in order to adjust first movable mirror's angle with respect to the support, second movable mirror installs in second adjusting device, second adjusting device drives second movable mirror rotates and/or removes with respect to the support in order to adjust second movable mirror's position and/or angle with respect to the support, the first movable mirror is installed in first movable mirror adjusting device, the dispersion compensation structure includes two dispersion compensation components are located between the dispersion compensation subassembly.

Further, the first adjusting device comprises a first mounting seat, a first worm and a first turbine, the first worm is rotatably mounted on the first mounting seat, the first turbine is meshed with the first worm, and the first movable reflector is mounted on the first worm.

Further, the support includes mounting panel, support column and bottom plate, the support column both ends respectively with the mounting panel with the bottom plate is fixed, first mount pad is fixed in the bottom plate, the mounting panel is equipped with movable groove, first movable reflector is located in the movable groove.

Further, the second adjusting device comprises a second mounting seat, a movable adjusting piece and a rotation adjusting assembly, the movable adjusting piece is mounted on the second mounting seat, the rotation adjusting assembly is connected with the movable adjusting piece, and the second movable reflecting mirror is mounted on the rotation adjusting assembly.

Further, the movable adjusting piece is a screw rod.

Further, the rotation adjusting assembly comprises a third mounting seat, a second worm and a second turbine, the third mounting seat is connected with the movement adjusting piece, the second worm is rotatably mounted on the third mounting seat, the second turbine is meshed with the second worm, and the second movable reflecting mirror is mounted on the second turbine.

Furthermore, the laser alignment structure further comprises an incidence aligner and an emergent aligner, wherein the incidence aligner and the emergent aligner are fixed on the support, the incidence aligner is used for calibrating the transmission direction of the incident laser, and the emergent aligner is used for calibrating the transmission direction of the emergent laser, so that the incident laser and the emergent laser are positioned on the same straight line.

Further, the first movable mirror is less distant from the incident aligner than the second movable mirror.

Further, the dispersion compensation assembly includes a prism and a grating, the prism being secured to the grating, the prism being oriented toward the movable mirror assembly.

Further, the dispersion compensation component further comprises a fourth adapter, the grating is mounted on the fourth adapter, the support is provided with a first mounting groove, and the fourth adapter is mounted in the first mounting groove.

Compared with the prior art, the first movable reflector of the laser pulse dispersion compensation device is arranged on the first adjusting device, the first adjusting device drives the first movable reflector to rotate relative to the bracket so as to adjust the angle of the first movable reflector relative to the bracket, the second movable reflector is arranged on the second adjusting device, the second adjusting device drives the second movable reflector to rotate and/or move relative to the bracket so as to adjust the position and/or angle of the second movable reflector relative to the bracket, the dispersion compensation structure comprises two dispersion compensation components, the movable reflector components are positioned between the two dispersion compensation components, and through the design, the first adjusting device and the second adjusting device adjust the positions and/or angles of the first movable reflector and the second movable reflector to control the angle of incidence of laser to the dispersion compensation structure, so that the transmission path of the laser in the dispersion compensation structure is changed, and the adjusting device compensates the dispersion amount of the laser pulse, so that the laser pulse dispersion compensation device has wider application scenes; the dispersion compensation device is embedded into the system, so that the system is convenient to apply, can be repeatedly applied, saves funds and saves resources.

Drawings

FIG. 1 is a perspective view of a laser pulse dispersion compensating device of the present utility model;

FIG. 2 is a partial perspective view of the laser pulse dispersion compensation device of FIG. 1;

FIG. 3 is another partial structural perspective view of the laser pulse dispersion compensating device of FIG. 1;

FIG. 4 is a perspective view of a first adjustment device of the laser pulse dispersion compensation device of FIG. 1;

FIG. 5 is a perspective view of a second adjustment device of the laser pulse dispersion compensation device of FIG. 1;

fig. 6 is an optical path diagram of the laser pulse dispersion compensating device of fig. 1.

In the figure: 10. a bracket; 11. a mounting plate; 110. a fixing hole; 111. a first mounting groove; 112. a movable groove; 113. a second mounting groove; 12. a support column; 13. a bottom plate; 20. a laser alignment structure; 21. an incidence aligner; 22. an exit aligner; 23. a movable mirror assembly; 231. a first movable mirror; 232. a first mirror; 233. a first adapter; 234. a second movable mirror; 235. a second mirror; 236. a second adapter; 24. a fixed mirror assembly; 241. a fixed mirror unit; 242. a third mirror; 243. a third adapter; 30. a dispersion compensation structure; 31. a dispersion compensation component; 310. a prism; 311. a grating; 312. a fourth adapter; 40. a first adjusting device; 41. a first mount; 42. a first worm; 43. a first turbine; 50. a second adjusting device; 51. a second mounting base; 52. moving the adjusting member; 53. a rotation adjustment assembly; 530. a third mount; 531. a second worm; 532. and a second turbine.

Detailed Description

The following description of the embodiments of the present utility model will be made clearly and completely with reference to the accompanying drawings, in which it is apparent that the embodiments described are only some embodiments of the present utility model, but not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the utility model without making any inventive effort, are intended to be within the scope of the utility model.

It will be understood that when an element is referred to as being "fixed to" another element, it can be directly on the other element or be present as another intermediate element through which the element is fixed. When an element is referred to as being "connected" to another element, it can be directly connected to the other element or intervening elements may also be present. When an element is referred to as being "disposed on" another element, it can be directly on the other element or intervening elements may also be present. The terms "vertical," "horizontal," "left," "right," and the like are used herein for illustrative purposes only.

Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this utility model belongs. The terminology used herein in the description of the utility model is for the purpose of describing particular embodiments only and is not intended to be limiting of the utility model. The term "and/or" as used herein includes any and all combinations of one or more of the associated listed items.

As shown in fig. 1 to 6, the laser pulse dispersion compensating apparatus of the present utility model includes a bracket 10, a laser alignment structure 20, a dispersion compensating structure 30, a first adjusting device 40, and a second adjusting device 50.

The bracket 10 is used to mount a laser alignment structure 20, a dispersion compensating structure 30, a first adjustment device 40, and a second adjustment device 50. The bracket 10 includes a mounting plate 11, support columns 12, and a base plate 13. Both ends of the support column 12 are fixed to the mounting plate 11 and the bottom plate 13, respectively. The mounting plate 11 is parallel to the base plate 13 and the support columns 12 are perpendicular to the mounting plate 11 and the base plate 13. The mounting plate 11 and the base plate 13 have thus far formed a receiving space for the first adjusting device 40 and the second adjusting device 50. Specifically, the number of the support columns 12 is four, and the four support columns 12 are respectively located at four corners of the mounting plate 11 and the bottom plate 13.

The mounting plate 11 is provided with a fixed hole 110, a first mounting groove 111, a movable groove 112, and a second mounting groove 113. The fixing holes 110 are used to fix the support columns 12. The fixing holes 110 are located at four corners of the mounting plate 11. The first mounting groove 111 is used for mounting the dispersion compensating structure 30. The number of the first mounting grooves 111 is two, and the two first mounting grooves 111 are parallel to each other. The two first mounting grooves 111 mount the two dispersion compensating elements 31, respectively. The movable groove 112 is located between the two first mounting grooves 111, and the movable mirror assembly 23 is located in the movable groove 112 and can rotate and move in the movable groove 112. The movable trough 112 includes two alignment angled slots for initial angular alignment of the first movable mirror 231 and the second movable mirror 234, the initial position alignment angled slots calibrating the initial angles of the first movable mirror 231 and the second movable mirror 234. The second mounting slot 113 is used to mount the fixed mirror assembly 24. The two second mounting grooves 113 are parallel to each other for mounting the two fixed mirror units 241, respectively.

The laser alignment structure 20 includes an entrance aligner 21, an exit aligner 22, a movable mirror assembly 23, and a fixed mirror assembly 24.

The incidence aligner 21 is fixed to the mounting plate 11 and is located at an end of the mounting plate 11. The incident aligner 21 performs alignment of the transmission direction of the incident laser light.

The exit aligner 22 is fixed to the mounting plate 11 and is located at the opposite end of the mounting plate 11. The emission aligner 22 performs alignment of the emission laser in the transmission direction. The incident laser and the emergent laser are positioned on the same straight line.

The movable mirror assembly 23 is used for controlling the angle of incidence of the laser to the dispersion compensation structure 30, changing the transmission path of the laser in the dispersion compensation structure 30, and adjusting the dispersion amount of the laser pulse compensation by the device. The movable mirror assembly 23 includes a first movable mirror 231 and a second movable mirror 234.

The first movable mirror 231 includes a first mirror 232 and a first adapter 233. The first reflecting mirror 232 is embedded in the first adapter 233, and when the first reflecting mirror 232 needs to be replaced, the first reflecting mirror 232 can be detached from the first adapter 233 and a new first reflecting mirror 232 can be installed by pulling and inserting. The first adapter 233 is connected to the first adjusting device 40, so that the first adjusting device 40 drives the first movable mirror 231 to rotate to adjust an angle. The first movable mirror 231 is movably installed in the movable groove 112 and the initial angle is calibrated by the alignment chute.

The second movable mirror 234 includes a second mirror 235 and a second adapter 236. The second mirror 235 is embedded in the second adapter 236. When the second reflecting mirror 235 needs to be replaced, the second reflecting mirror 235 can be detached from the second adapter 236 and a new second reflecting mirror 235 can be installed by pulling and inserting. The second adapter 236 is connected to the second adjusting device 50, and the second adjusting device 50 drives the second adapter 236 to rotate for adjusting an angle and move for adjusting a position. The second movable mirror 234 is movably mounted to the movable slot 112 and is calibrated for initial angle by aligning the chute.

The fixed mirror assembly 24 is placed at a specific angle (the angle is specially valued in order to maintain the transmission direction of the laser light before and after passing through the laser pulse dispersion compensation device), the fixed mirror assembly 24 changes the transmission direction of the laser light passing through the uniform transmission path of the movable mirror assembly 23 twice, the laser light is controlled to be emitted from the emergent aligner 22, and the transmission direction is the same as the incident laser light direction of the incident aligner 21. The fixed mirror assembly 24 includes two fixed mirror units 241, and the two fixed mirror units 241 are respectively installed in the second installation groove 113 and parallel to each other. Each of the fixed mirror units 241 includes a third mirror 242 and a third adapter 243, and the third mirror 242 is mounted to the third adapter 243.

The dispersion compensation structure 30 performs dispersion compensation on the femtosecond laser pulse transmitted by the set optical path trajectory. The dispersion compensating structure 30 comprises two dispersion compensating elements 31. Each dispersion compensating element 31 includes a prism 310, a grating 311, and a fourth adapter 312. The prism 310 is aligned with the upper plane of the grating 311 to form a prismatic grating structure, and the inclined plane of the prism 310 and the line surface of the grating 311 are glued surfaces, the grating 311 of the prismatic grating structure is longer than the prism 310, and the extended part of the grating 311 is inserted (glued) into the fourth adapter 312, and the fourth adapter 312 is embedded into the first mounting groove 111 of the mounting plate 11. Two dispersion compensating modules 31 are located on either side of the movable mirror module 23 and parallel to each other.

The first adjusting device 40 comprises a first mount 41, a first worm 42 and a first worm wheel 43. The first mount 41 is fixed to the bottom plate 13, the first worm 42 is rotatably mounted to the first mount 41, and the first worm wheel 43 is engaged with the first worm 42. The first movable mirror 231 is mounted to the first turbine 43. The first worm 42 rotates to drive the first turbine 43 to rotate, so that the first movable reflector 231 rotates relative to the bracket 10, and the angle of the first movable reflector 231 is adjusted.

The second adjusting device 50 includes a second mount 51, a moving adjusting member 52, and a rotating adjusting assembly 53. The second mount 51 is fixed to the base plate 13, and the movement adjuster 52 is rotatably mounted to the second mount 51. The rotation adjustment assembly 53 includes a third mount 530, a second worm 531, a second worm gear 532. The third mounting seat 530 cooperates with the movement adjusting member 52, so that the movement adjusting member 52 rotates to drive the third mounting seat 530 to translate. In this embodiment, the moving adjusting member 52 is a screw, and the third mounting seat 530 is connected to the screw through a nut. The second worm 531 is rotatably mounted to the third mount 530, the second worm wheel 532 is engaged with the second worm 531, and the second movable mirror 234 is connected to the second worm wheel 532. The movement adjusting piece 52 rotates to drive the second movable mirror 234 to translate the adjusting position, and the second worm 531 rotates to drive the second movable mirror 234 to rotate by an adjusting angle.

When the laser pulse dispersion compensation device of the present utility model is used, as shown in fig. 6, a laser pulse is incident from the incidence aligner 21, and the incidence aligner 21 aligns the transmission direction of the incident laser light. The laser pulse then enters the dispersion compensation component 31 after being reflected by the first movable mirror 231, and at this time, the first movable mirror 231 is used to control the angle of incidence of the laser beam on the dispersion compensation component 31, and change the transmission path of the laser beam on the dispersion compensation component 31, so as to adjust the dispersion amount for compensating the laser pulse. The dispersion compensation component 31 enters another dispersion compensation component 31 after being reflected, the other dispersion compensation component 31 is reflected and then is emitted to the second movable reflector 234, the second movable reflector 234 reflects the laser pulse to the fixed reflector unit 241, the fixed reflector unit 241 reflects the laser pulse to the other fixed reflector unit 241, at this time, the two fixed reflector units 241 change the transmission directions twice, the laser is controlled to be emitted from the emergent aligner 22, and the emergent aligner 22 calibrates the transmission directions of the emergent laser. At this time, the incident laser pulse and the emergent laser pulse are positioned on the same straight line.

When the laser pulse dispersion compensation device is used in different scenes, the dispersion compensation amounts are different, and at the moment, the first worm 42 rotates to drive the first turbine 43 to rotate, so that the first movable reflector 231 rotates relative to the bracket 10, and the angle of the first movable reflector 231 is adjusted. The movement adjusting piece 52 rotates to drive the second movable reflecting mirror 234 to translate and adjust the position, and the second worm 531 rotates to drive the second movable reflecting mirror 234 to rotate and adjust the angle, so that the dispersion compensation amount of the laser pulse dispersion compensation device is changed, and the laser pulse dispersion compensation device is suitable for different scenes.

In addition, the laser pulse dispersion compensation device has compact integral structure, convenient use, repeated application, capital and resource saving.

The foregoing examples illustrate only a few embodiments of the utility model, which are described in detail and are not to be construed as limiting the scope of the utility model. It should be noted that, for those skilled in the art, it is possible to make several modifications and improvements without departing from the concept of the present utility model, which are equivalent to the above embodiments according to the essential technology of the present utility model, and these are all included in the protection scope of the present utility model.

Claims

1. The utility model provides a laser pulse dispersion compensation arrangement, includes support, its characterized in that: the dispersion compensation device comprises a support, a first movable reflector, a second movable reflector, a dispersion compensation structure, a first adjusting device and a second adjusting device, wherein the first adjusting device drives the first movable reflector to rotate relative to the support to adjust the angle of the first movable reflector relative to the support, the second movable reflector is mounted on the second adjusting device, the second adjusting device drives the second movable reflector to rotate and/or move relative to the support to adjust the position and/or angle of the second movable reflector relative to the support, and the first movable reflector is mounted on the first adjusting device and drives the first movable reflector to rotate relative to the second movable reflector to adjust the position and/or angle of the second movable reflector relative to the support.

2. The laser pulse dispersion compensating device of claim 1, wherein: the first adjusting device comprises a first mounting seat, a first worm and a first turbine, the first worm is rotatably mounted on the first mounting seat, the first turbine is meshed with the first worm, and the first movable reflecting mirror is mounted on the first worm.

3. The laser pulse dispersion compensating device of claim 2, wherein: the support comprises a mounting plate, a support column and a bottom plate, wherein two ends of the support column are respectively fixed with the mounting plate and the bottom plate, the first mounting seat is fixed on the bottom plate, the mounting plate is provided with a movable groove, and the first movable reflector is positioned in the movable groove.

4. The laser pulse dispersion compensating device of claim 1, wherein: the second adjusting device comprises a second mounting seat, a movable adjusting piece and a rotation adjusting assembly, wherein the movable adjusting piece is mounted on the second mounting seat, the rotation adjusting assembly is connected with the movable adjusting piece, and the second movable reflecting mirror is mounted on the rotation adjusting assembly.

5. The laser pulse dispersion compensating device of claim 4, wherein: the movable adjusting piece is a screw rod.

6. The laser pulse dispersion compensating device of claim 4, wherein: the rotation adjusting assembly comprises a third mounting seat, a second worm and a second turbine, the third mounting seat is connected with the movable adjusting piece, the second worm is rotatably mounted on the third mounting seat, the second turbine is meshed with the second worm, and the second movable reflecting mirror is mounted on the second turbine.

7. The laser pulse dispersion compensating device according to any one of claims 1 to 6, wherein: the laser alignment structure further comprises an incidence aligner and an emergent aligner, wherein the incidence aligner and the emergent aligner are fixed on the support, the incidence aligner is used for calibrating the transmission direction of incident laser, and the emergent aligner is used for calibrating the transmission direction of emergent laser, so that the incident laser and the emergent laser are positioned on the same straight line.

8. The laser pulse dispersion compensating device of claim 7, wherein: the first movable mirror is less than the second movable mirror is less than the incident aligner.

9. The laser pulse dispersion compensating device according to any one of claims 1 to 6, wherein: the dispersion compensation assembly includes a prism secured to the grating, the prism oriented toward the movable mirror assembly.

10. The laser pulse dispersion compensating device of claim 9, wherein: the dispersion compensation component further comprises a fourth switching piece, the grating is installed in the fourth switching piece, the support is provided with a first installation groove, and the fourth switching piece is installed in the first installation groove.