CN219010295U - Sampling and collecting integrated device of fermentation tank - Google Patents

Abstract

The utility model discloses a sampling and collecting integrated device of a fermentation tank, and relates to the technical field of fermentation tank equipment. The stainless steel sampling pipeline is prolonged, the stainless steel sampling pipeline is tightly attached to the inner side of the air inlet pipeline, and the stainless steel sampling pipeline extends to the central position of the tank bottom while the ventilation of the air inlet is not influenced, so that the problem of incomplete sample collection is effectively solved; the four-way pipe is connected with the sampling pipe, one end of the four-way pipe is connected with the emptying hose, the residual pipe is left when the sampling pipe is emptied, the sampling port is arranged at one end of the four-way pipe, the lengthened sampling pipe is arranged at one side of the sampling pipe, the operation is convenient, the splashing is effectively prevented, and the environmental pollution is less; the sample collection and sampling share one stainless steel pipeline, so that the production cost of a fermentation tank manufacturer is effectively saved, the internal space of the fermentation tank is not occupied, and the space utilization rate is improved; the stainless steel pipeline is reduced in the fermentation tank, so that the sanitary dead angle is reduced, and the risk of fermentation pollution is effectively reduced.

Description

Sampling and collecting integrated device of fermentation tank

Technical Field

The utility model relates to the technical field of fermentation tank equipment, in particular to a sampling and collecting integrated device of a fermentation tank.

Background

The existing glass fermentation tank sampling device and the existing glass fermentation tank sampling device are separated. The sampling device mainly shows the following modes: a stainless steel sampling tube with single function is inserted into the fermentation tank, and the sampling depth does not reach the bottom of the tank; sampling in an emptying mode, and hydraulically returning fermentation liquid in a sampling pipeline into a fermentation tank through air pressure; the function is single, can't satisfy the receipts appearance needs simultaneously. The main change forms of the sample collection device are as follows: a stainless steel pipeline with a single function is inserted into the fermentation tank to be used as a lower tank sample collection, the depth of the pipeline is also the bottom of the tank, and a certain distance is reserved between the pipeline and the bottom of the tank; the first method is as follows: during sampling, due to the fact that the sampling hose outside the tank is short, workers are required to hold the hose and aim at the beaker, the tail gas pipeline is clamped by the hemostatic forceps or the tail gas outlet is plugged by fingers, and fermentation liquor is ejected out under the action of air pressure, so that the sample collection is carried out in the mode. The second method is as follows: the sample collection port is externally connected with a long silica gel hose and is matched with a peristaltic pump to extract and collect fermentation liquor. The third method is as follows: opening an inoculation port, putting a long silica gel hose into a fermentation tank, pumping fermentation liquor into a container by matching with a peristaltic pump, and taking out the hose for cleaning and sterilizing after sample collection is completed.

The first method has the main disadvantages that: 1. because the depth of the sampling pipeline is insufficient and does not reach the center of the tank bottom, fermentation liquor cannot be completely taken out during sample collection, and the sampling pipeline is inconvenient; 2. the flow rate cannot be controlled during sample collection, the flow rate is too slow, the research and development progress is affected, the flow rate is too fast, the container causes splashing, the environment is polluted, and the waste is caused; 3. when receiving the appearance, the sampling port pipeline often length is insufficient, needs to have the staff hold the mouth of pipe always, extravagant manpower.

The second three methods have the main disadvantages: additional equipment is needed to be matched with sample collection, if a peristaltic pump integrated with a fermentation tank is used, the problems of too low rotation speed and insufficient pipe diameter are caused, the sample collection time is prolonged, and the production efficiency is reduced; or extra peristaltic pumps with high rotating speed and large pipe diameters are purchased, so that the investment of research and development equipment is increased, and a certain burden is caused to enterprise operation.

The third method has the main disadvantages that: 1. in the sample collection process, the position of the silica gel hose is changed all the time due to vibration of the peristaltic pump, and a worker is required to adjust along with displacement of the pipeline, otherwise, a fermentation sample cannot be completely collected due to the change of the hose, so that labor cost is increased; 2. after the sampling is finished, the hose is taken out, part of residues exist in the hose and outside the hose, the hose is dripped on the surface of a laboratory table, the laboratory environment is polluted, a cleaning and disinfecting program is added, and the labor is wasted.

The three methods have the common disadvantages that: 1. the sample collection and sampling can not be combined, a sampling pipeline and a sample collection pipeline are required to be respectively arranged, and the production cost of the fermentation tank is increased; 2. with the increase of the pipelines, the more the dead angles are cleaned, the more the pollution possibility is.

Disclosure of Invention

The utility model aims to provide a sampling and collecting integrated device of a fermentation tank, which is used for solving the problems in the background technology.

In order to solve the technical problems, the utility model provides the following technical scheme: the utility model provides a sample and receipts appearance integrated device of fermentation cylinder, includes the glass jar body and air compressor, the inside extension receipts sampling tube and the in-tank air pipeline that is equipped with of glass jar, extension receipts sampling tube one end extends to the inside bottom of glass jar in the jar, extension receipts sampling tube other end extends to the outside of glass jar and is equipped with the cross connection in the jar, the interface connection of cross connection has the sample connection, the interface connection of cross connection has extension receipts appearance hose, air compressor passes through air cleaner and three way connection's interface connection, in-tank air pipeline one end extends to the external side of glass jar and three way connection's interface connection, three way connection passes through the connecting pipe and is connected with the cross connection.

Further, the connecting pipe outer wall cover is equipped with a pipe clamp, extension receipts appearance hose outer wall cover is equipped with No. two pipe clamps.

Further, the inside stirring frame that rotates to be connected that is equipped with of glass jar, glass jar body top is equipped with servo motor, servo motor output shaft and stirring frame top center fixed connection.

Further, the in-tank lengthened sampling pipeline and the in-tank air pipeline are both arranged on one side of the inner part of the glass tank body, and one end of the in-tank air pipeline extends to the lower part of the stirring frame.

Further, a tail gas hose is arranged on one side of the top of the glass tank body, and an infusion roller, a tail gas filter and a tail gas condenser are arranged on the tail gas hose.

Further, the infusion roller is sleeved on the outer wall of the tail gas hose, the infusion roller is arranged between the tail gas filter and the tail gas condenser, and the tail gas condenser is arranged at one end, close to the glass tank body, of the tail gas hose.

Compared with the prior art, the utility model has the following beneficial effects:

1. the utility model is provided with a glass tank body, an air compressor, a lengthened sampling pipe in the tank, an air pipe in the tank and a lengthened sampling hose; the functions of sampling and collecting samples are realized by utilizing a stainless steel pipeline in the fermentation tank; lengthening the sample collection hose, preventing splashing caused by too short pipeline, releasing manpower, and only putting the pipeline into a container without being held by personnel all the time; a second pipe clamp is normally closed and is in a sealing state; loosening the lower tank when collecting samples, and collecting the samples through a sample collecting hose; the sampling pipeline is lengthened in the tank and is clung to the inner side of the air inlet pipeline, and the sampling pipeline extends to the central position of the tank bottom while the ventilation of the air inlet is not influenced; the problem of incomplete sample collection in the lower tank is effectively solved; the transfusion roller is required to be filled with compressed air in the fermentation process, and oxygen is supplied to the thalli and the positive pressure of the tank body is maintained; the infusion roller is additionally arranged at the tail gas hose, and the compressed air at the inner diameter of the tail gas pipeline, namely the air inlet, is extruded, and cannot be normally discharged after being utilized by the thallus reaction; so fermentation broth is through the effect of compressed air atmospheric pressure, reverse follow receives appearance mouth outflow, thereby control tail gas break-make and gas size control receipts appearance switch and velocity of flow, prevent to lead to sample liquid to spray because of the air current is too big, need not additionally to use peristaltic pump.

2. The stainless steel sampling pipeline is prolonged, the stainless steel sampling pipeline is tightly attached to the inner side of the air inlet pipeline, and the stainless steel sampling pipeline extends to the central position of the tank bottom while the ventilation of the air inlet is not influenced, so that the problem of incomplete sample collection is effectively solved; the four-way pipe is connected with the sampling pipe, one end of the four-way pipe is connected with the emptying hose, the residual pipe is left when the sampling pipe is emptied, the sampling port is arranged at one end of the four-way pipe, the lengthened sampling pipe is arranged at one side of the sampling pipe, the operation is convenient, the splashing is effectively prevented, and the environmental pollution is less; the infusion roller is added at the tail gas hose, the sample collection flow rate can be controlled under the action of air pressure, the production efficiency is improved, a peristaltic pump is not required to be additionally used, the sample collection is performed by using self-source energy of the fermentation tank, and the cost of research and development equipment is saved; the sample collection and sampling share one stainless steel pipeline, so that the production cost of a fermentation tank manufacturer is effectively saved, the internal space of the fermentation tank is not occupied, and the space utilization rate is improved; the stainless steel pipeline is reduced in the fermentation tank, so that the sanitary dead angle is reduced, and the risk of fermentation pollution is effectively reduced.

Drawings

The accompanying drawings are included to provide a further understanding of the utility model and are incorporated in and constitute a part of this specification, illustrate the utility model and together with the embodiments of the utility model, serve to explain the utility model. In the drawings:

FIG. 1 is a schematic view of the overall structure of the present utility model;

in the figure: 1. a servo motor; 2. an air compressor; 3. a sampling port; 4. an air filter; 5. a three-way joint; 6. a first pipe clamp; 7. a four-way joint; 8. lengthening the sample collection hose; 9. a second pipe clamp; 10. an in-tank air duct; 11. lengthening a sampling pipeline in the tank; 12. infusion idler wheels; 13. an exhaust gas filter; 14. a tail gas condenser; 15. a stirring rack; 16. a glass tank body.

Detailed Description

The following description of the embodiments of the present utility model will be made clearly and completely with reference to the accompanying drawings, in which it is apparent that the embodiments described are only some embodiments of the present utility model, but not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the utility model without making any inventive effort, are intended to be within the scope of the utility model.

Referring to fig. 1, the present utility model provides the following technical solutions: the utility model provides a sample and receipts appearance integrated device of fermentation cylinder, includes glass jar body 16 and air compressor 2, the inside extension receipts sampling tube 11 and the in-tank air duct 10 that are equipped with of glass jar body 16, the extension receipts sampling tube 11 one end in jar extends to the inside bottom of glass jar body 16, the extension receipts sampling tube 11 other end in jar extends to the outside of glass jar body 16 and is equipped with cross joint 7, the 1 interface of cross joint 7 has sampling port 3, the 2 interface of cross joint 7 has extension receipts appearance hose 8, air compressor 2 passes through air cleaner 4 and the 1 interface connection of three way joint 5, the extension of in-tank air duct 10 one end extends to the outside of glass jar body 16 and the 2 interface connection of three way joint 5, three way joint 5 passes through the connecting pipe and is connected with cross joint 7; the connecting pipe outer wall cover is equipped with pipe clamp No. 6, extension receipts appearance hose 8 outer wall cover is equipped with pipe clamp No. 9.

A stirring frame 15 in rotary connection is arranged in the glass tank 16, a servo motor 1 is arranged above the glass tank 16, and an output shaft of the servo motor 1 is fixedly connected with the center of the top of the stirring frame 15; the in-tank lengthened sampling pipeline 11 and the in-tank air pipeline 10 are both arranged on one side of the interior of the glass tank body 16, and one end of the in-tank air pipeline 10 extends to the lower part of the stirring frame 15; a tail gas hose is arranged on one side of the top of the glass tank body 16, and an infusion roller 12, a tail gas filter 13 and a tail gas condenser 14 are arranged on the tail gas hose; the infusion roller 12 is sleeved on the outer wall of the tail gas hose, the infusion roller 12 is arranged between the tail gas filter 13 and the tail gas condenser 14, and the tail gas condenser 14 is arranged at one end, close to the glass tank 16, of the tail gas hose.

The working principle of the utility model is as follows:

referring to the attached figure 1 of the specification, the utility model carries out stirring treatment on fermentation liquor in the glass tank body 16 by arranging the glass tank body 16, the air compressor 2, the in-tank lengthened sampling pipe 11, the in-tank air pipe 10 and the lengthened sampling hose 8, and driving the stirring frame 15 to carry out rotary motion by the servo motor 1; an air compressor 2 for supplying oxygen to the fermentation cells and maintaining a positive pressure in the tank; the sampling port 3 is matched with a sampler (a disposable threaded injector) by using a threaded positive pressure venous transfusion connector and a normally closed type, so that the tightness is better and the pollution is reduced; air filter 4: one end of the three-way valve is connected with an air compressor, the compressed air is sterilized and filtered through an air filter to obtain sterile compressed air, required air and oxygen are provided for fermentation thalli, the inside of the tank body is kept at positive pressure to prevent fermentation pollution caused by negative pressure, and the other end of the three-way valve is connected with a three-way joint 5; one end of the three-way joint 5 is connected with the air filter 4, the other end is connected with the air pipeline 10 in the tank, and the other end is connected with the four-way joint 7; and a first pipe clamp 6 for controlling the tee joint and the four-way connecting hose, and loosening the pipe clamp after the sampling is finished, so that the residue in the collecting pipeline is returned to the fermentation tank. Waste is reduced, yield is increased, and research and development production cost is saved; the four-way joint 7 is connected with a tee joint at one end, a sampling pipeline in the tank at one end, a sampling port at one end and a lengthened sampling pipeline at one end; the functions of sampling and collecting samples are realized by utilizing a stainless steel pipeline in the fermentation tank; the sample collection hose 8 is lengthened, so that the splashing problem caused by too short pipeline is prevented, meanwhile, the manpower is liberated, the personnel does not need to hold the sample collection hose all the time, and the pipeline is only required to be put into a container; the second pipe clamp 9 is normally closed and is in a sealing state; loosening the lower tank when collecting samples, and collecting the samples through a sample collecting hose; the sampling pipeline 11 is lengthened in the tank and is clung to the inner side of the air inlet pipeline, and the sampling pipeline extends to the central position of the tank bottom while the ventilation of the air inlet is not influenced; the problem of incomplete sample collection in the lower tank is effectively solved; the transfusion roller 12 is used for supplying compressed air to the thalli and maintaining positive pressure of the tank body in the fermentation process; the infusion roller 12 is additionally arranged at the tail gas hose, and the compressed air at the inner diameter of the tail gas pipeline, namely the air inlet, is extruded, and cannot be normally discharged after being utilized by the thallus reaction; the fermentation liquid flows out from the sample collecting port reversely under the action of the air pressure of the compressed air, and the on-off state of tail gas and the gas size of the tail gas are controlled so as to control the sample collecting switch and the flow rate, so that the sample liquid is prevented from being sprayed due to overlarge air flow, and a peristaltic pump is not required to be additionally used;

sampling: taking a disposable sterile threaded injector, inserting the disposable sterile threaded injector into the sampling port 3, extracting a required fermentation liquid sample, taking out the injector, and detecting various parameters of the sample; opening a first pipe clamp 6, and enabling air to pass through a connecting pipe connected between the tee joint and the four joint, so that residual fermentation liquor in a sampling pipe in the fermentation tank returns to the fermentation tank under the action of air pressure, and the residual of a pipeline is avoided; slowly extracting a small amount of residual sample liquid in the outer sampling port hose by using a disposable sterile spiral injector; closing the first pipe clamp 6 during the next sampling, and circulating the actions;

when the sample is collected: closing the first pipe clamp 6 to enable the sampling pipe to be free from air; opening a second pipe clamp 9, and putting the lengthened sample receiving hose 8 into a sample receiving container; moving the infusion roller 12, the tail gas hose is pressed, and the tail gas cannot be normally discharged. At this time, the fermentation liquor is ejected out through the reverse action of the gas; the transfusion roller 12 is regulated, the air flow of the tail gas hose is changed along with the change of the air flow, and the volume of the fermentation liquid ejected from the sample receiving port is also changed along with the change of the air flow, so that the size of the sample output can be controlled, and a proper flow rate is selected until the sample receiving is finished;

the stainless steel sampling pipeline is prolonged, the stainless steel sampling pipeline is tightly attached to the inner side of the air inlet pipeline, and the stainless steel sampling pipeline extends to the central position of the tank bottom while the ventilation of the air inlet is not influenced, so that the problem of incomplete sample collection is effectively solved; the four-way pipe is connected with the sampling pipe, one end of the four-way pipe is connected with the emptying hose, the residual pipe is left when the sampling pipe is emptied, the sampling port is arranged at one end of the four-way pipe, the lengthened sampling pipe is arranged at one side of the sampling pipe, the operation is convenient, the splashing is effectively prevented, and the environmental pollution is less; the infusion roller is added at the tail gas hose, the sample collection flow rate can be controlled under the action of air pressure, the production efficiency is improved, a peristaltic pump is not required to be additionally used, the sample collection is performed by using self-source energy of the fermentation tank, and the cost of research and development equipment is saved; the sample collection and sampling share one stainless steel pipeline, so that the production cost of a fermentation tank manufacturer is effectively saved, the internal space of the fermentation tank is not occupied, and the space utilization rate is improved; the stainless steel pipeline is reduced in the fermentation tank, so that the sanitary dead angle is reduced, and the risk of fermentation pollution is effectively reduced.

Finally, it should be noted that: the foregoing description is only a preferred embodiment of the present utility model, and the present utility model is not limited thereto, but it is to be understood that modifications and equivalents of some of the technical features described in the foregoing embodiments may be made by those skilled in the art, although the present utility model has been described in detail with reference to the foregoing embodiments. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present utility model should be included in the protection scope of the present utility model.

Claims (6)

1. The utility model provides a sample and collection appearance integrated device of fermentation cylinder, includes glass jar body (16) and air compressor (2), its characterized in that: the inside extension of glass jar body (16) is equipped with jar in-tank sampling pipeline (11) and jar air duct (10), jar in-tank extension sampling pipeline (11) one end extends to glass jar body (16) inboard bottom, jar in-tank extension sampling pipeline (11) other end extends to glass jar body (16) outside and is equipped with cross joint (7), the 1 interface connection of cross joint (7) has sample connection (3), the 2 interface connection of cross joint (7) has extension to receive appearance hose (8), air compressor (2) are through 1 interface connection of air cleaner (4) with three way connection (5), jar in-tank air duct (10) one end extends to the 2 interface connection of glass jar body (16) outside and three way connection (5), three way connection (5) are connected with cross joint (7) through the connecting pipe.

2. The fermenter sampling and sampling integrated device according to claim 1, wherein: the outer wall of the connecting pipe is sleeved with a first pipe clamp (6), and the outer wall of the lengthened sample collection hose (8) is sleeved with a second pipe clamp (9).

3. The fermenter sampling and sampling integrated device according to claim 1, wherein: the stirring frame (15) connected in a rotating way is arranged inside the glass tank body (16), a servo motor (1) is arranged above the glass tank body (16), and an output shaft of the servo motor (1) is fixedly connected with the top center of the stirring frame (15).

4. A fermenter sampling and collection integrated device according to claim 3, wherein: the in-tank lengthened sampling pipeline (11) and the in-tank air pipeline (10) are both arranged on one side of the inside of the glass tank body (16), and one end of the in-tank air pipeline (10) extends to the lower part of the stirring frame (15).

5. The fermenter sampling and sampling integrated device according to claim 1, wherein: a tail gas hose is arranged on one side of the top of the glass tank body (16), and an infusion roller (12), a tail gas filter (13) and a tail gas condenser (14) are arranged on the tail gas hose.

6. The integrated sampling and collection device for a fermenter according to claim 5, wherein: the infusion roller (12) is sleeved on the outer wall of the tail gas hose, the infusion roller (12) is arranged between the tail gas filter (13) and the tail gas condenser (14), and the tail gas condenser (14) is arranged at one end, close to the glass tank body (16), of the tail gas hose.

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