CN218811667U - Experimental orifice plate for quantifying scratches of cells - Google Patents
Experimental orifice plate for quantifying scratches of cells Download PDFInfo
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- CN218811667U CN218811667U CN202223321554.0U CN202223321554U CN218811667U CN 218811667 U CN218811667 U CN 218811667U CN 202223321554 U CN202223321554 U CN 202223321554U CN 218811667 U CN218811667 U CN 218811667U
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Abstract
The utility model provides an experiment orifice plate for quantifying cell mar, include: the culture medium fixing device comprises a base, a plurality of holes are formed in the base, one or more detachable partition plates are vertically arranged in each hole from the bottom of each hole to the opening end of each hole, the detachable partition plates are tightly matched and connected with the bottoms of the holes, and a combination area which cannot be penetrated by a culture medium is formed between each detachable partition plate and the bottom of each hole; the height of the detachable partition board is smaller than the depth of the hole, and the width of the detachable partition board is smaller than or equal to the aperture of the hole. The utility model discloses a set up the baffle dismantled of vertical installation inside the hole for the cell can form the mar of regular length, width and degree of depth naturally when growing in the hole, does not need artifical cutting, has improved work efficiency, has guaranteed simultaneously cell mar test time scratch size's each time unified, makes the quantitative calculation more convenient, has also improved the precision of quantitative calculation.
Description
Technical Field
The utility model relates to an experimental ware technical field specifically, relates to an experiment orifice plate for quantifying cell mar.
Background
Cell scratch experiments are a routine method of measuring cell migration movement and repair ability. In the existing cell scratching experiment, after cells grow in a pore plate, a trace gun head or other hard objects are used for scribing in a specified cell growth area for testing, and the method generally causes that the obtained scribing length, width and depth are different, and the calculation accuracy is seriously influenced when quantitative calculation is carried out.
SUMMERY OF THE UTILITY MODEL
The utility model discloses to the above-mentioned not enough that exists among the prior art, provide an experiment orifice plate for quantifying cell mar.
The utility model discloses a realize through following technical scheme.
The utility model provides a pair of an experiment orifice plate for quantifying cell mar, include: the culture medium fixing device comprises a base, a plurality of holes are formed in the base, one or more detachable partition plates are vertically arranged in each hole from the bottom of each hole to the opening end of each hole, the detachable partition plates are tightly matched and connected with the bottoms of the holes, and a combination area which cannot be penetrated by a culture medium is formed between each detachable partition plate and the bottom of each hole; the height of the detachable partition plate is smaller than the depth of the hole, and the width of the detachable partition plate is smaller than or equal to the aperture of the hole.
Optionally, the detachable spacer has a thickness of 500 μm.
Optionally, the bottom in hole be equipped with can dismantle the unanimous mounting groove of baffle quantity, can dismantle the baffle the bottom be equipped with the installation department of mounting groove size looks adaptation, the installation department with interference fit connects between the mounting groove.
Optionally, the number of the detachable partition boards is three, and the three detachable partition boards are arranged in parallel.
Optionally, the plurality of removable baffles are uniform in width and/or height.
Optionally, the detachable partition plate and the bottom of the hole are perpendicular to each other.
Optionally, the assay well plate further comprises a cover, the cover being detachably mounted on the base.
Compared with the prior art, the utility model discloses at least one following beneficial effect has:
the utility model discloses above-mentioned embodiment provides an experiment orifice plate for quantifying cell mar is through the baffle dismantled that sets up vertical installation inside the hole for the cell can form the mar of fixed length, width and degree of depth naturally when growing in the hole, does not need artifical cutting, has improved work efficiency, has guaranteed the unification of mar size when cell mar is experimental every time simultaneously, makes the quantitative calculation more convenient, has also improved the precision of quantitative calculation.
The utility model discloses the experiment orifice plate for quantifying cell mar that above-mentioned embodiment provided, simple structure can realize on the basis that does not change current orifice plate infrastructure promptly, need not be familiar with the orifice plate operation again, suitable popularization.
Drawings
Other features, objects and advantages of the invention will become more apparent upon reading of the detailed description of non-limiting embodiments with reference to the following drawings:
fig. 1 is a top view of an experimental well plate for quantifying cell scratch according to a preferred embodiment of the present invention.
Fig. 2 is a front view of an experimental well plate for quantifying cell scratches according to a preferred embodiment of the present invention; wherein, (a) and (c) are structural schematic diagrams that the width of the detachable clapboard is smaller than the aperture of the hole, and (b) and (d) are structural schematic diagrams that the width of the detachable clapboard is equal to the aperture of the hole.
Fig. 3 is a schematic view of the holes in a preferred embodiment of the present invention.
Fig. 4 is a schematic structural view of a detachable partition plate according to a preferred embodiment of the present invention.
Fig. 5 is a schematic view of the installation of the detachable partition in the hole according to a preferred embodiment of the present invention.
In the figure, 1 is a base, 2 is a hole, 3 is a detachable clapboard, 4 is a cover body, 5 is a mounting groove, and 6 is a mounting part.
Detailed Description
The following is a detailed description of embodiments of the present invention: this embodiment is using the utility model discloses technical scheme carries out under the prerequisite, has given detailed implementation mode and specific operation process. It should be noted that, for those skilled in the art, without departing from the spirit of the present invention, several variations and modifications can be made, which are within the scope of the present invention.
An embodiment of the utility model provides an experiment orifice plate for quantifying cell mar, this experiment orifice plate is through setting up the baffle dismantled of vertical installation inside the hole for the cell can form the mar of fixed length, width and degree of depth naturally when growing in the hole.
As shown in fig. 1, the experimental well plate for quantifying cell scratch provided in this embodiment may include: the culture medium fixing device comprises a base 1, wherein a plurality of holes 2 are formed in the base 1, one or more detachable partition plates 3 are vertically arranged in each hole 2 from the bottom of each hole 2 to the opening end of each hole 2, the detachable partition plates 3 are tightly matched and connected with the bottoms of the holes 2, and a combination area which cannot be penetrated by a culture medium is formed between each detachable partition plate 3 and the bottom of each hole 2; the height of the detachable partition board 3 is less than the depth of the hole 2, and the width of the detachable partition board 3 is less than or equal to the aperture of the hole 2, as shown in fig. 2; by means of the detachable partition 3, the scratch with fixed length, width and depth can be formed when the cell grows in the hole.
In a preferred embodiment, the thickness of the removable partition 3 is 500 μm.
In a preferred embodiment, the bottom of the hole 2 is provided with mounting slots 5 with the same number as the detachable partition plates 3, as shown in fig. 3, the bottom end of the detachable partition plate 3 is provided with a mounting part 6 with the size matched with that of the mounting slot 5, and the mounting part 6 is connected with the mounting slot 5 in an interference fit manner, as shown in fig. 5. Through interference fit between installation department and the mounting groove and be connected for closely cooperate between the bottom in detachable partition board and hole, the unable infiltration of bonding area culture medium of formation, and then make around culture solution and cell can't get into, form the mar of fixed length, width and degree of depth automatically in cell production process.
In a preferred embodiment, there are three removable partitions 3, and the three removable partitions 3 are arranged in parallel with each other. A plurality of detachable partition boards 3 are arranged in the same hole, so that the utilization rate of cells can be increased.
In a preferred embodiment, the widths and/or heights of the plurality of removable spacers 3 may or may not be uniform, and in the quantitative analysis, it is ensured that the thickness of the removable spacers 3 is fixed to 500 μm.
In a preferred embodiment, the removable partition 3 is arranged perpendicular to the bottom of the cavity 2.
In a preferred embodiment, the assay well plate may further comprise a lid 4, the lid 4 being detachably mounted on the base 1.
The utility model discloses an experiment orifice plate for quantifying cell mar that above-mentioned embodiment provided, its theory of operation is:
at the inside vertical installation of hole one can dismantle the baffle, and the cell grows in the hole, and after filling up whole hole, dismantle the baffle, just can form the mar of a fixed length, width and degree of depth naturally on the cell, adopt the utility model discloses the above-mentioned embodiment provides an experiment orifice plate does not need artifical cutting, has improved work efficiency, has guaranteed the unification of mar size when cell mar is experimental each time simultaneously for the quantitative calculation is more convenient, has also improved the precision of quantitative calculation.
In the description of the present invention, it is to be understood that the terms "upper", "lower", "vertical", "horizontal", "top", "bottom", "inner", "outer", etc. indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings, and are merely intended to simplify the description of the present invention, rather than to indicate or imply that the device or element so referred to must have a particular orientation, be constructed and operated in a particular orientation, and thus, should not be construed as limiting the present invention.
The above embodiments of the present invention are not the best known techniques in the art.
The foregoing description of the specific embodiments of the invention has been presented. It is to be understood that the present invention is not limited to the specific embodiments described above, and that various changes and modifications may be made by those skilled in the art within the scope of the appended claims without departing from the spirit of the invention.
Claims (7)
1. An experimental well plate for quantifying cell scratch, comprising: the culture medium base comprises a base (1), wherein a plurality of holes (2) are formed in the base (1), and the culture medium base is characterized in that one or more detachable partition plates (3) are vertically arranged in each hole (2) from the bottom of each hole (2) to the opening end of each hole (2), the detachable partition plates (3) are tightly matched and connected with the bottoms of the holes (2), and a bonding area where a culture medium cannot permeate is formed between the detachable partition plates and the bottoms of the holes (2); the height of the detachable partition plate (3) is smaller than the depth of the hole (2), and the width of the detachable partition plate (3) is smaller than or equal to the aperture of the hole (2).
2. The assay well plate for quantifying cell scratches according to claim 1, wherein the thickness of the removable partition (3) is 500 μm.
3. The utility model is used for quantifying experimental orifice plate of cell mar according to claim 1, characterized in that, the bottom of hole (2) be equipped with can dismantle the unanimous mounting groove (5) of baffle (3) quantity, the bottom of dismantling baffle (3) be equipped with the installation department (6) of mounting groove (5) size looks adaptation, installation department (6) with interference fit is connected between mounting groove (5).
4. The well plate for quantifying cell scratches according to claim 1, wherein the number of said detachable partition plates (3) is three, and three of said detachable partition plates (3) are disposed in parallel with each other.
5. The lab well plate for quantifying cell scratches according to claim 1, wherein the width and/or height of the plurality of removable baffles (3) is uniform.
6. The assay well plate for quantifying cell scratches according to claim 1, wherein the detachable partition (3) is arranged perpendicular to the bottom of the hole (2).
7. The assay well plate for quantifying cell scratches according to any one of claims 1 to 4, further comprising a lid (4), wherein the lid (4) is detachably mounted on the base (1).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202223321554.0U CN218811667U (en) | 2022-12-12 | 2022-12-12 | Experimental orifice plate for quantifying scratches of cells |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202223321554.0U CN218811667U (en) | 2022-12-12 | 2022-12-12 | Experimental orifice plate for quantifying scratches of cells |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN218811667U true CN218811667U (en) | 2023-04-07 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202223321554.0U Active CN218811667U (en) | 2022-12-12 | 2022-12-12 | Experimental orifice plate for quantifying scratches of cells |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN218811667U (en) |
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2022
- 2022-12-12 CN CN202223321554.0U patent/CN218811667U/en active Active
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