CN2186755Y

CN2186755Y - Multipurpose culture plate

Info

Publication number: CN2186755Y
Application number: CN 94227350
Authority: CN
Inventors: 莫兵
Original assignee: Chengdu Institute of Biology of CAS
Current assignee: Chengdu Institute of Biology of CAS
Priority date: 1994-03-25
Filing date: 1994-03-25
Publication date: 1995-01-04
Anticipated expiration: 2004-03-25

Abstract

The utility model relates to a multipurpose culture plate, which solve the problems that the original cell culture plate has inconvenient operation, and is adverse to culture single-cell and is unable to co-culture multiple cells. The multipurpose culture plate is characterized in that at least two straight through hole culture cavities (2) are arranged on the culture plate (1); the bottom of the culture cavity (2) is a screen hole bottom (3) which can be fixed or detachable. The multipurpose culture plate is widely suitable for single cell or organization culture and multiple cells or organizations co-culture, and has the advantages of simple operation, multiple purposes, etc. The utility model is a novel culture plate.

Description

Multipurpose culture plate

The utility model is a kind of multi-usage culture plate, belongs to the further improvement to general Tissue Culture Plate.

Common Tissue Culture Plate commonly used has several bottoms to be fully closed cultivation cave on it, in use the weak point of Cun Zaiing is:

1, when carrying out single cell culture, for preventing the evaporation of nutrient solution, need on the nutrient solution surface, to add one deck mineral oil, therefore to influence the normal gaseous interchange of culture inevitably, culture can only utilize initial nutritive medium growth, can not get replenishing of fresh nutrition; Metabolic refuse also can only be accumulated in the suitable narrow space, can't spread apart, and is unfavorable for the division and the growth of cell.

2, can not be used for the common cultivation of various kinds of cell.

3, can not be used for the common cultivation of tissue culture and multiple tissue.

Also the someone adopts polypropylene screen or poly fat sieve aperture sheet as carrier now, though solved the problem of nutrient solution evaporation and nutritional supplementation and refuse diffusion, can not divide septal cell effectively, is difficult to guarantee unicity.

The purpose of this utility model be to avoid the deficiencies in the prior art and provide a kind of and place culture dish, it cultivates the multi-usage culture plate that cave is the air-permeable bottom, with the needs of the common cultivation of satisfying unicellular or tissue culture and multiple class cell or tissue.

The purpose of this utility model can reach by following measure: have at least two to cultivate caves (2) on the culture plate (1), cultivating cave (2) be clear opening, and the bottom in cultivation cave (2) is (3) at the bottom of the sieve aperture, and screen-aperture is less than the yardstick of culture.

The purpose of this utility model can also reach by following measure: the bottom surface in culture plate (1), each cultivates the outer fluted (7) in cave (2), two porous plates (8) is arranged, its diameter equals the diameter of culture plate (1), its aperture is corresponding one by one with cultivation cave (2) with the position, accompany filter screen (or millipore filtration) between two porous plates, above on porous plate, there is a flange (9) at place, outer along each hole, the height of edge and width match with the degree of depth and the width of the outer groove (7) of cultivation cave (2), and flange (9) can closely be tipped in the groove (7); The height H of cultivating cave (2) is the height h of culture plate (1), on the outer wall of the evagination bottom in cultivation cave positive Luo line (5) is arranged, corresponding to each evagination bottom hole wall of cultivating the cave one cylinder is arranged, cloudy Luo line (6) is arranged on the cylinder inner wall and match with positive Luo line (5) on cultivating cave evagination bottom outer wall, the base mouth diameter d of each cylinder is less than barrel bore D, and filter screen (4) (or filter membrane) places on the base of cylinder.Culture plate preferably adopts transparent material to make.The shape and size of culture plate are advisable can put into culture vessel, and for general culture dish, culture plate is best with the disc-shaped structure.

Description of drawings:

Fig. 1 is the utility model structure iron.

Fig. 2 is embodiment 1 a sieve aperture bear building-up composition.

Fig. 3 is embodiment 2 structure iron.

Fig. 4 is embodiment 2 sieve aperture bear building-up compositions.

Fig. 5 is embodiment 3 culture plate structure iron.

Fig. 6 is embodiment 3 culture plate bottom surface structure iron.

Fig. 7 is the bottom cylindrical structure figure of embodiment 3.

Fig. 8, Fig. 9 are embodiment 4 culture plate structure iron.

Figure 10, Figure 11 are embodiment 4 sieve aperture bear building-up compositions.

The utility model is described in further detail below in conjunction with embodiment:

Embodiment 1, as shown in Figure 1: have 4 to cultivate cave (2) on the culture plate (1), cultivating cave (2) be circular clear opening, with tackiness agent the cellulose acetate millipore filtration (4) of one aperture, 0.65 μ m is sticked on the whole bottom of culture plate (1), (3) (as shown in Figure 2) at the bottom of the formation sieve aperture;

Embodiment 2, as shown in Figure 3: have several round tube holes to cultivate cave (2) on the culture plate (1), with tackiness agent 400 order nylon leaching net sheets are pasted the bottom of cultivating cave (2), (3) (as shown in Figure 4) at the bottom of the formation sieve aperture.

Embodiment 3, as Fig. 5, shown in Figure 6: as to have 4 to cultivate cave (2) on the culture plate (1), cultivate the height h of the height H in cave (2) greater than culture plate (1), on the outer wall of the evagination bottom in cultivation cave positive Luo line (5) is arranged, corresponding to each evagination bottom hole wall of cultivating the cave one cylinder (as shown in Figure 7) is arranged, cloudy Luo line (6) is arranged on the cylinder inner wall and match with positive Luo line (5) on the evagination bottom outer wall, the base mouth diameter d that makes each cylinder is less than barrel bore D, filter screen (4) (or filter membrane) places on the base of cylinder, cylinder is screwed on the hole wall of evagination bottom, cultivation cave, interlock by the Luo line, cultivate evagination bottom, cave hole wall filter screen (4) or filter membrane sheet are pressed on the cylinder base, thereby constitute at the bottom of the detachable sieve aperture.

Embodiment 4, and as Fig. 8, shown in Figure 9: have 4 to cultivate caves (2) on the culture plate (1), the height of cultivating cave (2) equals the height of culture plate (1), in the bottom surface of culture plate (1), the outer that each cultivates cave (2) one groove (7) arranged.Form (as shown in figure 10) by two porous plates (8) at the bottom of the sieve aperture, its diameter equals the diameter of culture plate (1), and its aperture is corresponding one by one with cultivation cave (2) with the position, accompanies filter screen (4) (or millipore filtration) between two porous plates.Above on porous plate, there is a flange (9) at place, outer along each hole, the width of flange and height match with the width and the degree of depth of the outer groove (7) of cultivating cave (2), flange (9) can closely be tipped in the groove (7), thus (3) (as shown in figure 11) at the bottom of the sieve aperture that formation can be for convenience detach.

The utility model is compared prior art and is had following advantage:

Be conducive to single celled cultivation, during use, with the unicellular cultivation cave that places Multipurpose culture plate, whole culture plate being placed to fill culture medium and cultivate has the culture dish of a large amount of this kind cells to cultivate again, each cultivate be in cave one unicellular, each is cultivated cave and communicates with nutrient solution at the bottom of by its sieve aperture, can exchange material and information between the cell, each cell is in one enough among the big cell colony like this, satisfy very simply the requirement of floor cells density, be very beneficial for single celled division and growth. Secondly, because these sieve apertures communicate with a large amount of nutrient solutions in the culture dish, the nutrient solution in the cave can not dry up because of evaporation, therefore do not need that the nutrient solution surface adds the paraffin oil that avoids evaporating in the cave, thereby be conducive to the gas exchange between cell and the environment, the 3rd, because training Nutrient solutions a large amount of in a large amount of sieve apertures of supporting the ware bottom and the culture dish communicate, can obtain continuously replenishing of fresh culture, metabolic waste also can be diffused in the culture dish by sieve aperture simultaneously, thereby obtains dilution, and this obviously is conducive to the g and D of cell.

Be conducive to the multiple types cell or tissue and cultivate altogether, different types of cell or tissue is placed respectively different cultivation cave on the good same Multipurpose culture plate of mark, thereby very simply different types of cell or tissue is separated, it is not polluted. Place culture dish to cultivate Multipurpose culture plate, the chemical substance that different types of cell or tissue produces can be diffused in the culture dish by sieve aperture, see through again sieve aperture and be diffused into cell or tissue, division, differentiation, growth, the metabolism of cell or tissue played a role, realize cultivating altogether. Perhaps a certain purpose cell or tissue is placed the cultivation cave of culture plate, and its hetero-organization or cell are placed culture dish, place culture dish to cultivate culture plate then, can create suitable " ecological environment " for a certain purpose cell or tissue, reach best cultivation purpose.

Under the single cell culture and the situation of cultivating altogether, it is very easy to change the liquid operation, only needs culture plate changed in the culture dish that fills fresh culture to get final product.

If desired, in culture dish, put into little bar magnet, culture dish is placed on the magnetic stirring apparatus, can in culturing process, stir nutrient solution.

Claims

1, a kind of multi-usage culture plate is made up of the cultivation cave on culture plate and its, it is characterized in that having on the culture plate (1) at least two to cultivate caves (2), and cultivating cave (2) is clear opening, is (3) at the bottom of the sieve aperture bottom it, and screen-aperture is less than the yardstick of culture.

2, multi-usage culture plate as claimed in claim 1 is characterized in that (3) are made of filter screen (4) at the bottom of the sieve aperture, and filter screen is 400 orders～500 orders.

3, multi-usage culture plate as claimed in claim 1 is characterized in that being made of millipore filtration (4) at the bottom of the sieve aperture, and the filter membrane aperture is 0.05 μ m～10 μ m.

4, multi-usage culture plate as claimed in claim 1 is characterized in that at the bottom of the sieve aperture be dismountable.

5, as claim 1 or 2 or 3 or 4 described multi-usage culture plates, it is characterized in that cultivating the height h of the height H in cave (2) greater than culture plate (1), on the outer wall of the evagination bottom in cultivation cave positive Luo line (5) is arranged, corresponding to each evagination bottom hole wall of cultivating the cave one cylinder is arranged, cloudy Luo line (6) is arranged on the cylinder inner wall and match with positive Luo line (5) on cultivating cave evagination bottom outer wall, the base mouth diameter d of each cylinder is less than barrel bore D, and filter screen (4) (or filter membrane) places on the base of cylinder.

6, as claim 1 or 2 or 3 or 4 described multi-usage culture plates, it is characterized in that bottom surface in culture plate (1), each cultivates the outer fluted (7) in cave (2), two porous plates (8) is arranged, its diameter equals the diameter of culture plate (1), its aperture and position are corresponding one by one with cultivation cave (2), accompany filter screen (4) (or millipore filtration) between two porous plates (8), above on porous plate, there is flange (9) at place, outer along each hole, the width of flange and height match with the width and the degree of depth of the outer groove (7) of cultivating cave (2), and flange (9) can closely be tipped in the groove (7).

7, multi-usage culture plate as claimed in claim 1 is characterized in that the multi-usage culture plate preferably adopts nontoxic transparent material to make, and its shape and its size are advisable can put into culture vessel.