CN218665869U - Shrouding membrane and PCR hot lid seal membrane structure - Google Patents
Shrouding membrane and PCR hot lid seal membrane structure Download PDFInfo
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- CN218665869U CN218665869U CN202223435295.4U CN202223435295U CN218665869U CN 218665869 U CN218665869 U CN 218665869U CN 202223435295 U CN202223435295 U CN 202223435295U CN 218665869 U CN218665869 U CN 218665869U
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Abstract
The utility model provides a shrouding membrane and PCR heat cover sealing membrane structure. The sealing plate film comprises a pyrolysis viscosity loss adhesive layer, a base material layer and a sealing plate adhesive layer which are sequentially arranged from top to bottom, wherein the pyrolysis viscosity loss temperature of the pyrolysis viscosity loss adhesive layer is 80-95 ℃, and the sealing plate adhesive layer still keeps certain adhesive force at 20-105 ℃. When the sealing membrane is applied to a PCR instrument, the sealing membrane of the sample pore plate can be sealed only by adhering the sealing membrane to the hot cover and closing or pressurizing the hot cover; because the temperature of the hot cover reaches the pyrolysis viscosity losing temperature in the PCR amplification process, after the PCR is finished, the hot cover can be separated from the sealing plate film after being opened; before PCR detects, need not to carry out the membrane sealing operation to the sample orifice plate specially earlier, accomplish membrane sealing and amplification detection in step on the PCR appearance, promoted PCR detection efficiency greatly.
Description
Technical Field
The utility model relates to a seal membrane technical field, specifically be a shrouding membrane and PCR heat blanket seal membrane structure.
Background
In order to prevent the sample from escaping during the detection of the biological sample, it is usually necessary to seal the orifice of the reagent tube containing the sample with a sealing plate membrane. The membrane sealing instrument is an instrument for sealing membranes, and the current membrane sealing step is to firstly virtually place a sealing plate membrane on a sample pore plate and then place the sample pore plate membrane into the membrane sealing instrument for membrane sealing. However, the position of the sealing plate film on the sample pore plate is unstable before the film sealing, and the situation of the displacement of the sealing plate film can occur when the moving process is easily scratched with a film sealing instrument or influenced by air flow, so that the film sealing is unqualified. The unqualified film sealing is torn off and the film sealing process is carried out again, so that samples are easily splashed, and the experiment efficiency is influenced.
In addition, in the commonly used PCR (polymerase chain reaction) detection, it is usually necessary to seal the sample well plate first, and then place the sealed sample well plate into the PCR instrument for amplification detection. For a laboratory, a film sealing instrument and a PCR instrument need to be configured synchronously, and configuration cost is increased. The membrane is sealed firstly and then the detection is carried out, so that the steps are multiple, and the PCR detection efficiency is influenced.
SUMMERY OF THE UTILITY MODEL
In order to overcome the defects of the prior art, the utility model aims to provide a sealing membrane and a PCR hot cap sealing membrane structure.
In order to achieve the above object, the technical solution of the present invention is: the utility model provides a shrouding membrane, includes pyrolysis debonding glue layer, substrate layer and the shrouding glue layer that from top to bottom sets gradually, pyrolysis debonding temperature on pyrolysis debonding glue layer is 80-95 ℃.
By adopting the technical scheme of the utility model, when the sealing film device is applied, before the sealing film, the pyrolysis debonding glue layer of the sealing film is firstly attached to the upper cover plate with the heating function of the sealing film device; when the membrane is sealed, the upper cover plate and the sealing plate are pressed on the upper surface of the pore plate to be sealed under the membrane, and the sealing plate adhesive layer is adhered to the pore plate to be sealed; the temperature of the upper cover plate is continuously raised while the film is sealed, the upper cover plate is moved away after the temperature reaches the viscosity losing temperature, the upper cover plate is separated from the sealing plate film, and the film sealing of the sample pore plate is completed. Because the upper cover plate is more accurate with the position location that the seat was placed to the orifice plate, the shrouding membrane is also more accurate in the attached position on the upper cover plate, and the dislocation can not take place for the shrouding membrane and sample orifice plate like this, has promoted the membrane quality of sealing greatly. When the sealing membrane is applied to a PCR instrument, the sealing membrane of the sample pore plate can be sealed only by adhering the sealing membrane to the hot cover and closing or pressurizing the hot cover; because the temperature of the hot cover reaches the pyrolysis viscosity loss temperature in the PCR amplification process, the hot cover can be separated from the sealing plate film after being opened after the PCR is finished; before PCR detection, the film sealing operation of the sample pore plate is not required to be specially carried out, the film sealing and the amplification detection are synchronously completed on a PCR instrument, and the PCR detection efficiency is greatly improved.
Furthermore, the sealing plate glue layer can maintain certain adhesive force at least in the temperature range of 20-105 ℃.
Further, still include cover in the last release film of pyrolysis debonding glue layer upper surface, and cover in the lower release film of shrouding glue layer lower surface.
Adopt above-mentioned preferred scheme, from the type membrane to protect pyrolysis debonding glue film and shrouding glue film from the top down, convenient turnover.
Further, the upper release film and the lower release film are different in color.
Furthermore, marks for distinguishing the upper surface and the lower surface of the sealing plate film are arranged on the upper release film and the lower release film.
By adopting the preferable scheme, the upper surface and the lower surface of the sealing plate film are conveniently distinguished, and reverse misuse is prevented.
Further, the pyrolysis viscosity loss temperature of the pyrolysis viscosity loss adhesive layer is 90 ℃.
The preferable scheme is more suitable for the use of the hot-cover sealing film of the PCR instrument.
Furthermore, the pyrolysis viscose loss glue layer is composed of a plurality of small pyrolysis viscose loss glue units which are isolated from each other.
Further, the small thermal desorption glue units are in a dot shape, a round shape or a square shape.
Adopt above-mentioned preferred scheme, conveniently when the pad pasting, exhaust, improve the closure plate membrane and cover attached roughness on the heat.
The utility model provides a PCR hot lid envelope structure, includes hot lid, heating element and closing plate membrane, the heating element is installed hot lid upper surface or inlay and locate in the hot lid, the subsides of pyrolysis viscidity glue layer upper surface of closing plate membrane are located the lower surface of hot lid.
By adopting the scheme, when the sealing membrane is applied to a PCR instrument, the sealing membrane of the sample pore plate can be sealed only by sticking the sealing membrane on the hot cover and closing or pressurizing the hot cover; because the temperature of the hot cover reaches the pyrolysis viscosity loss temperature in the PCR amplification process, the hot cover can be separated from the sealing plate film after being opened after the PCR is finished; before PCR detects, need not to carry out the membrane sealing operation to the sample orifice plate specially earlier, accomplish membrane sealing and amplification detection in step on the PCR appearance, promoted PCR detection efficiency greatly.
Furthermore, the heat cover comprises a heat cover main body and a film relay board, the film relay board is detachably mounted on the heat cover main body, and the upper surface of the pyrolysis adhesive loss layer of the sealing board film is attached to the lower surface of the film relay board.
By adopting the preferable scheme, the film-sealing film is positioned and attached through the film-pasting relay board which is arranged separately from the hot cover main body, so that the film-pasting convenience is improved, and the circulation and turnover are convenient.
Further, the lower surface of the film pasting relay board is provided with a benchmark for pasting the sealing board film.
By adopting the preferable scheme, the film pasting alignment is facilitated, and the film sealing position precision is improved.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly described below, it is obvious that the drawings in the following description are only some embodiments of the present invention, and for those skilled in the art, other drawings can be obtained according to these drawings without creative efforts.
FIG. 1 is a cross-sectional view of one embodiment of a closure membrane of the present invention;
FIG. 2 is a cross-sectional view of another embodiment of the closure membrane of the present invention;
FIG. 3 is a top view of another embodiment of the closure membrane of the present invention;
FIG. 4 is a schematic view of the placement of one attachment of the sealing plate film on the bottom surface of the hot lid;
FIG. 5 is a schematic diagram showing the position of a sealing plate film attached to a PCR instrument;
FIG. 6 is a schematic view of another embodiment of the sealing plate film attached to the bottom surface of the hot lid film relay board;
FIG. 7 is a schematic diagram showing the position of another plate sealing film attached to the PCR instrument.
Names of corresponding parts represented by numerals and letters in the drawings:
10-sealing plate film; 11-pyrolytic debonding adhesive layer; 111-small thermal viscose loss unit; 12-a substrate layer; 13-sealing plate glue layer; 14-applying a release film; 15-lower release film; 21-a thermal cover; 211-thermal cap body; 212-film relay board; 2121-reference; 22-a heating element; 23-a pressurizing mechanism; a 24-orifice plate heating seat assembly; 31-well plate.
Detailed Description
The technical solutions in the embodiments of the present invention will be described clearly and completely with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by a person skilled in the art without making creative efforts belong to the protection scope of the present invention.
As shown in fig. 1, a sealing plate film includes a pyrolytic viscosity-loss adhesive layer 11, a substrate layer 12, and a sealing plate adhesive layer 13, which are sequentially disposed from top to bottom, wherein the pyrolytic viscosity-loss temperature of the pyrolytic viscosity-loss adhesive layer 11 is 80-95 ℃.
The beneficial effect of adopting above-mentioned technical scheme is: when the adhesive is applied to a film sealing instrument, before film sealing, the pyrolytic debonding adhesive layer of the sealing plate film is firstly attached to an upper cover plate with a heating function of the film sealing instrument; when the membrane is sealed, the upper cover plate and the sealing plate are pressed on the upper surface of the pore plate to be sealed under the membrane, and the sealing plate adhesive layer is bonded with the pore plate to be sealed; the temperature of the upper cover plate is continuously raised while the film is sealed, the upper cover plate is moved away after the temperature reaches the viscosity losing temperature, the upper cover plate is separated from the sealing plate film, and the film sealing of the sample pore plate is completed. Because the upper cover plate is more accurate with the position location that the seat was placed to the orifice plate, the shrouding membrane is also more accurate in the attached position on the upper cover plate, and the dislocation can not take place for the shrouding membrane and sample orifice plate like this, has promoted the membrane quality of sealing greatly. When the sample pore plate sealing film is applied to a PCR instrument, as shown in FIG. 5, the sealing film of the sample pore plate can be sealed only by adhering the sealing film 10 to the hot cover 21 and closing or pressurizing the hot cover; because the temperature of the hot cover reaches the pyrolysis viscosity loss temperature in the PCR amplification process, the hot cover can be separated from the sealing plate film after being opened after the PCR is finished; before PCR detects, need not to carry out the membrane sealing operation to the sample orifice plate specially earlier, accomplish membrane sealing and amplification detection in step on the PCR appearance, promoted PCR detection efficiency greatly.
The utility model discloses in, the concrete material of pyrolysis adhesive losing layer 11 can be followed prior art and obtained, if adopt the high temperature pyrolysis adhesive losing film that Kunshan right Ricai electronics Limited company sold. Alternatively, a pyrolytic debonding adhesive tape is also disclosed in the patent document with the application publication number of CN 105131860A, wherein a pyrolytic debonding adhesive layer and the principle thereof are described, a heating expandable foaming expandable microsphere foaming agent is embedded in the pyrolytic debonding adhesive layer, the expandable microsphere foaming agent is of a core-shell structure, a shell is a thermoplastic acrylic resin polymer, a core is a microsphere particle composed of alkane gas, the diameter of the particle is 10-45 micrometers, the temperature range of microsphere foaming is 75-260 ℃, and the most suitable microsphere type can be selected according to various processing temperatures and process requirements.
In the present invention, the specific material of the substrate layer 12 is not limited, and can be selected from the prior art, such as PET or other high-strength substrates.
The utility model discloses in, shrouding glue film 13 all keeps certain adhesive force at normal atmospheric temperature and high temperature, still keeps certain adhesive force at 20-105 ℃ at least, and the adhesive force setting of current conventional single face shrouding membrane can be referred to the concrete size of adhesive force. The material of the sealing plate adhesive layer is not limited, and can be selected from the prior art, such as acrylic adhesive glue or other adhesive glue layers with adhesive force.
As shown in fig. 2, in other embodiments of the present invention, the present invention further comprises an upper release film 14 covering the upper surface of the pyrolytic debonding layer 11, and a lower release film 15 covering the lower surface of the sealing plate glue layer 13. The beneficial effect of adopting above-mentioned technical scheme is: the pyrolysis adhesive losing layer and the sealing plate adhesive layer are protected by the upper release film and the lower release film, so that the turnover is convenient.
In other embodiments of the present invention, the upper release film 14 and the lower release film 15 have different colors in order to distinguish the areas where the pyrolytic adhesive loss layer and the sealing plate adhesive layer are located.
In other embodiments of the present invention, the upper release film 14 and the lower release film 15 are provided with marks for distinguishing the upper surface and the lower surface of the sealing plate film.
In other embodiments of the present invention, the pyrolysis debonding temperature of the pyrolysis debonding layer 11 is 90 ℃, which is more suitable for the use of the thermal cover sealing film of the PCR instrument.
In other embodiments of the present invention, as shown in fig. 3, the thermal de-bonding layer 11 is composed of a plurality of thermal de-bonding small units 111 isolated from each other. The shape of the thermal desorption adhesive patch 111 may be dotted, circular, square, etc. The beneficial effect of adopting above-mentioned technical scheme is: the convenience is when the pad pasting, exhausts, improves the attached roughness of closing plate membrane on the hot lid.
As shown in fig. 4 and 5, a PCR thermal cover sealing film structure includes a thermal cover 21, a heating element 22 and a sealing plate film 10, wherein the heating element 22 is installed on the upper surface of the thermal cover 21 or embedded in the thermal cover 21, and the upper surface of the thermal de-bonding adhesive layer 11 of the sealing plate film is attached to the lower surface of the thermal cover 21.
As shown in FIG. 5, the utility model discloses the closure membrane can be used on current PCR appearance, and the concrete structure of PCR appearance is no longer repeated, can follow prior art and acquire. In general, the PCR instrument includes a hot lid assembly including a hot lid 21, a heating element 22, and a pressurizing mechanism 23, and an orifice plate heating holder assembly 24 located below the hot lid assembly. When the sample pore plate sealing film is applied to a PCR instrument, the sealing film of the sample pore plate can be sealed only by adhering the sealing film 10 to the lower surface of the hot cover 21 and closing or pressurizing the hot cover 21; because the temperature of the hot cover 21 reaches the pyrolysis viscosity loss temperature in the PCR amplification process, after the PCR is finished, the hot cover 21 can be separated from the sealing plate film 10 after being opened; before PCR detects, need not to carry out the membrane sealing operation to the sample orifice plate specially earlier, accomplish membrane sealing and amplification detection in step on the PCR appearance, promoted PCR detection efficiency greatly.
As shown in fig. 6 and 7, in other embodiments of the present invention, the thermal cap 21 includes a thermal cap main body 211 and a film relay board 212, the film relay board 212 is detachably mounted on the thermal cap main body 211, and the upper surface of the thermal decomposition release adhesive layer 11 of the sealing plate film is attached to the lower surface of the film relay board 212. The beneficial effect of adopting above-mentioned technical scheme is: the sealing plate film and the film pasting relay board are pasted firstly and then are inserted and matched to the hot cover main body together, so that the film pasting convenience is improved, and the circular turnover is convenient.
As shown in fig. 6, in other embodiments of the present invention, the lower surface of the film relay board 212 is provided with a reference 2121 for attaching the cover film 10. The beneficial effect of adopting above-mentioned technical scheme is: the film pasting alignment is facilitated, and the film sealing position precision is improved.
The above embodiments are only for illustrating the technical conception and the features of the present invention, and the purpose thereof is to enable those skilled in the art to understand the contents of the present invention and implement the present invention, and the protection scope of the present invention can not be limited thereby, and all equivalent changes or modifications made according to the spirit of the present invention should be covered in the protection scope of the present invention.
Claims (10)
1. The sealing plate film is characterized by comprising a pyrolysis adhesive losing layer, a base material layer and a sealing plate adhesive layer which are sequentially arranged from top to bottom, wherein the pyrolysis adhesive losing temperature of the pyrolysis adhesive losing layer is 80-95 ℃.
2. A sealing plate film as claimed in claim 1 further comprising an upper release film covering the upper surface of the pyrolytic debonding adhesive layer and a lower release film covering the lower surface of the sealing plate adhesive layer.
3. A sealing plate film according to claim 2 wherein the upper and lower release films are of different colours.
4. A sealing plate film according to claim 2 wherein the upper and lower release films are provided with indicia for distinguishing the upper and lower surfaces of the sealing plate film.
5. A sealing plate membrane according to claim 1, wherein said layer of pyrolytic debonding glue has a pyrolytic debonding temperature of 90 ℃.
6. A sealing plate film as claimed in claim 1 wherein said layer of thermally debonding adhesive is comprised of a plurality of isolated thermally debonding adhesive cells.
7. A sealing plate film according to claim 6 wherein said thermally debonding adhesive cells are in the form of dots, circles or squares.
8. A PCR hot cap sealing film structure, which comprises a hot cap, a heating element and the sealing film of claim 1, wherein the heating element is mounted on the upper surface of the hot cap or embedded in the hot cap, and the upper surface of the pyrolytic adhesive loss layer of the sealing film is attached to the lower surface of the hot cap.
9. The PCR heat cover sealing film structure of claim 8, wherein the heat cover comprises a heat cover main body and a film relay board, the film relay board is detachably mounted on the heat cover main body, and the upper surface of the pyrolytic release adhesive layer of the sealing film is attached to the lower surface of the film relay board.
10. The PCR heat cover sealing film structure according to claim 9, wherein the lower surface of the film attaching relay board is provided with a reference for attaching a sealing film.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202223435295.4U CN218665869U (en) | 2022-12-21 | 2022-12-21 | Shrouding membrane and PCR hot lid seal membrane structure |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202223435295.4U CN218665869U (en) | 2022-12-21 | 2022-12-21 | Shrouding membrane and PCR hot lid seal membrane structure |
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| CN218665869U true CN218665869U (en) | 2023-03-21 |
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| CN202223435295.4U Active CN218665869U (en) | 2022-12-21 | 2022-12-21 | Shrouding membrane and PCR hot lid seal membrane structure |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115851149A (en) * | 2022-12-21 | 2023-03-28 | 莫纳(武汉)生物科技有限公司 | Sealing plate film and PCR (polymerase chain reaction) hot cap sealing method |
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2022
- 2022-12-21 CN CN202223435295.4U patent/CN218665869U/en active Active
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115851149A (en) * | 2022-12-21 | 2023-03-28 | 莫纳(武汉)生物科技有限公司 | Sealing plate film and PCR (polymerase chain reaction) hot cap sealing method |
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