CN218620864U

CN218620864U - Cell culture box convenient to change culture medium

Info

Publication number: CN218620864U
Application number: CN202222992826.3U
Authority: CN
Inventors: 张晗; 李立东
Original assignee: Beijing Taidong Biotechnology Co ltd
Current assignee: Beijing Taidong Biotechnology Co ltd
Priority date: 2022-11-10
Filing date: 2022-11-10
Publication date: 2023-03-14
Anticipated expiration: 2032-11-10

Abstract

The utility model discloses a cell culture box convenient for replacing culture medium, which comprises a box body, wherein the upper surface of the box body is provided with a culture tank with an upward opening end and a waste liquid tank, a baffle is arranged at the middle position between the culture tank and the waste liquid tank, one end of a culture tube is inserted into the inner wall of the bottom surface of the culture tank, the other end of the culture tube extends to the outer wall of the bottom surface of the box body and is provided with an external thread A, and the external thread A is in threaded connection with a screw cap; the utility model discloses a have the function of carrying out the cultivation to the cell, and can in time change the operation to the culture medium when the culture medium needs to be changed, need not to adopt centrifugal mode to get rid of the culture medium, avoided taking place the damage of cell at the removal culture medium in-process, it is convenient with the equipment still to have realized the dismantlement of this culture box, and has made things convenient for and has carried out the regular cleaning sterilization to culture tank and waste liquid inslot portion, this culture box simple structure and practicality are stronger, are fit for being extensively promoted and use.

Description

Cell culture box convenient to change culture medium

Technical Field

The utility model belongs to the technical field of cell culture, specifically be a cell culture box convenient to change culture medium.

Background

In recent years, the research and development of cell therapy are rapid, and in a plurality of fields including immune cell therapy, stem cell therapy and the like, the in vitro maintenance culture of cells is an indispensable technical means. Lymphocytes are used as suspension cells separated from blood, the operation of in vitro maintenance and amplification culture of the cells is complex, and the adopted cell culture bottle is not suitable for quickly replacing a cell culture medium. Conventional lymphocyte culture generally adopts T25, T75 and T225 cell culture flasks, and the isolated and purified lymphocytes are added into the culture flasks containing the lymphocyte culture medium.

At present, under the condition that the nutrient of a culture medium is insufficient and even exhausted, a cell suspension needs to be taken out to a centrifuge tube, and then lymphocytes and the culture medium are separated by a centrifugal method, so that the purposes of removing the nutrient-deficient culture medium and adding a fresh culture medium are achieved; however, the process of the technology is complicated in culture medium replacement and cell sample operation and treatment, and centrifugation can increase cell damage, so that the technology is not suitable for large-scale industrialized cell culture; for this reason, it is necessary to design a cell culture cassette that facilitates the exchange of culture medium.

Disclosure of Invention

To the above-mentioned circumstances, under the present circumstances at the culture medium nutrient deficiency or even exhaust, need take out the cell suspension to the centrifuging tube, rethread centrifugal method separation lymphocyte and culture medium, in order to reach the purpose of getting rid of the nutrient deficiency culture medium and adding fresh culture medium, but this kind of technique is on culture medium change and cell sample operation handles, and the process is more loaded down with trivial details, and the centrifugation can increase the cell damage, is not suitable for the cell culture problem of extensive industrialization, the utility model provides a cell culture box convenient to change the culture medium, effectual realization has the function of cultivateing the cell, and can in time change the operation to the culture medium when the culture medium needs to be changed, need not to adopt centrifugal mode to get rid of the culture medium, has avoided getting rid of the damage that the cell takes place at the culture medium in-process, has still realized the dismantlement and convenient equipment of this culture box, and has made things convenient for regularly to wash the sterilization culture tank and waste liquid inslot portion, and this culture box simple structure and practicality are stronger.

In order to achieve the above object, the utility model provides a following technical scheme: the utility model provides a cell culture box convenient to change culture medium, comprises a box bod, ascending culture tank of open end and waste liquid groove are seted up to the box body upper surface, just culture tank and waste liquid groove intermediate position are provided with the baffle, it has the one end of culture tube to peg graft on the culture tank bottom surface inner wall, just the other end of culture tube extends to box body bottom surface outer wall and has seted up external screw thread A, external screw thread A threaded connection has the screw cap, waste liquid groove bottom surface inner wall pegs graft has the one end of waste liquid pipe, just the other end of waste liquid pipe extends to box body bottom surface outer wall and has seted up external screw thread B, external screw thread B threaded connection has sealed cap, culture tank and waste liquid groove open end outer wall block are connected with the apron.

Preferably, the outer side wall of the cover plate is provided with a side plate, the outer side wall of the side plate is provided with a connecting hole B, and the outer wall of the bottom surface of the cover plate is provided with a sealing gasket.

Preferably, the outer side wall of the partition plate is provided with a filter membrane, the aperture of the filter membrane is 1-4 micrometers, and the distance between the filter membrane and the inner wall of the bottom surface of the culture tank is smaller than the distance between the filter membrane and the upper surface of the box body.

Preferably, the outer side wall of the box body is provided with a limiting plate, and the outer side wall of the limiting plate is provided with a connecting hole A.

Preferably, the inner wall of the bottom surface of the screw cap is provided with a breathable film, and the pore diameter of the breathable film is 0.45 micrometer.

Preferably, the number of the limiting plates is two, the side plates are located in the middle of the limiting plates, and the connecting holes A and the connecting holes B are detachably connected through external fasteners.

Preferably, the box body right side outer wall is provided with assists the fagging, just assist the fagging and the lateral width of limiting plate the same.

Preferably, the volume of the culture tank is larger than the volume of the waste liquid tank.

Compared with the prior art, the beneficial effects of the utility model are that:

(1) At first can be with sealed bottom surface outer wall card income along culture tank and waste liquid groove open end, and make apron bottom surface outer wall and box body upper surface laminating, meanwhile the curb plate can insert to the limiting plate intermediate position, thereby can rotate external fastener along connecting hole A and connecting hole B and can dismantle the connection between connecting hole A and the connecting hole B, and then just also can dismantle the connection between limiting plate and the curb plate, just so accomplished the sealed operation of culture tank and waste liquid groove, the effectual dismantlement and the equipment that has realized this culture tank are convenient, and made things convenient for and regularly cleaned the sterilization to culture tank and waste liquid inslot portion.

(2) First, the apron upper surface with the culture box is laminated with the holding surface upper surface, the rethread culture tube can add culture medium and cell mixture to the culture tank inside, can seal the culture tube along the rotatory screw cap of external screw thread A after that, make the air can freely get into the culture tank inside along the culture tube and carry out the cell culture operation under the ventilated membrane effect that screw cap bottom surface inner wall was provided with like this, when needs change the culture medium of culture tank inside, at this moment can add the solution that is used for the culture medium infiltration to the waste liquid inslot along the waste liquid pipe inside, the rethread is sealed along the rotatory sealed cap of external screw thread B can be sealed with waste liquid pipe lower extreme, can place the horizontal level of box body along limiting plate and supplementary backup pad afterwards, meanwhile can permeate culture medium waste liquid to the waste liquid inslot portion under the filter membrane effect that the baffle lateral wall was provided with, rethread rotatory sealed and can be discharged the waste liquid of waste liquid inslot by the waste liquid pipe, the effectual device that has realized has the function of carrying out the cultivation to the cell culture, and can in time carry out the operation to the culture medium when the culture medium needs to be changed, the mode that need not to adopt gets rid of getting rid of and get rid of the culture medium, this culture medium is got rid of the centrifugation, this strong practicality and the simple structure of taking place of the simple and the process.

Drawings

The accompanying drawings are included to provide a further understanding of the invention, and are incorporated in and constitute a part of this specification, illustrate embodiments of the invention, and together with the description serve to explain the invention and not to limit the invention. In the drawings:

fig. 1 is a schematic view of the overall structure of the present invention;

FIG. 2 is a schematic structural view of the outer side wall of the box body of the present invention;

FIG. 3 is a schematic view of the internal structure of the culture tank of the present invention;

fig. 4 is a schematic overall front view structure diagram of the present invention;

FIG. 5 is a schematic view of the cover plate structure of the present invention;

in the figure: 1. a box body; 2. a culture tank; 3. a partition plate; 4. a waste liquid tank; 5. a limiting plate; 6. a connecting hole A; 7. auxiliary supporting plates; 8. a waste liquid pipe; 9. an external thread B; 10. a sealing cap; 11. a culture tube; 12. an external thread A; 13. a screw cap; 14. a cover plate; 15. a gasket; 16. a side plate; 17. a connecting hole B; 18. a gas permeable membrane; 19. and (5) filtering the membrane.

Detailed Description

The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments; based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative efforts all belong to the protection scope of the present invention.

Examples

Given by fig. 1-5, the utility model relates to a cell culture box convenient to change culture medium, including box body 1, ascending culture tank 2 of open end and waste liquid groove 4 have been seted up to box body 1 upper surface, just culture tank 2 and waste liquid groove 4 intermediate position are provided with baffle 3, the one end that has culture tube 11 is pegged graft to 2 bottom surface inner walls of culture tank, just culture tube 11's the other end extends to 1 bottom surface outer wall of box body and has seted up external screw thread A12, external screw thread A12 threaded connection has screw cap 13, waste liquid groove 4 bottom surface inner wall is pegged graft there is waste liquid pipe 8's one end, just waste liquid pipe 8's the other end extends to 1 bottom surface outer wall of box body and has seted up external screw thread B9, external screw thread B9 threaded connection has sealing cap 10, culture tank 2 and waste liquid groove 4 open end outer wall block are connected with apron 14, can add the solution that is used for culture medium infiltration to waste liquid groove 4 inside through along waste liquid pipe 8, and the rethread can seal the waste liquid pipe 8 lower extreme with can seal waste liquid pipe 8 sealing plate along external screw thread B9 rotatory sealing cap 10 from and can seal up waste liquid pipe 8 lower extreme, and then can place the horizontal infiltration limiting plate, and this waste liquid groove 3 inside the limiting plate 19 at this waste liquid groove 3.

Specifically, the 14 lateral walls of apron are provided with curb plate 16, just connecting hole B17 has been seted up to the 16 lateral walls of curb plate, 14 bottom surface outer walls of apron are provided with sealed the pad 15, can block the 15 bottom surface outer walls of sealed pad into through along culture tank 2 and waste liquid groove 4 open end to make 14 bottom surface outer walls of apron and the laminating of box body 1 upper surface.

Specifically, the outer side wall of the partition board 3 is provided with a filter membrane 19, the aperture of the filter membrane 19 is 1-4 microns, the distance between the filter membrane 19 and the inner wall of the bottom surface of the culture tank 2 is smaller than the distance between the filter membrane 19 and the upper surface of the box body 1, and the distance between the filter membrane 19 and the inner wall of the bottom surface of the culture tank 2 is smaller than the distance between the filter membrane 19 and the upper surface of the box body 1, so that the culture box can culture cells by utilizing the space inside the culture tank 2 as much as possible.

Specifically, 1 lateral wall of box body is provided with limiting plate 5, just connecting hole A6 has been seted up to 5 lateral walls of limiting plate, thereby can carry out preliminary block between apron 14 and the box body 1 and be connected through inserting curb plate 16 to 5 intermediate positions of limiting plate.

Specifically, the inner wall of the bottom surface of the screw cap 13 is provided with a breathable film 18, the pore diameter of the breathable film 18 is 0.45 microns, and air can freely enter the culture tank 2 along the culture tube 11 to perform cell culture operation under the action of the breathable film 18 arranged on the inner wall of the bottom surface of the screw cap 13.

Specifically, the figure of limiting plate 5 is two, just curb plate 16 is located limiting plate 5 intermediate position, can dismantle the connection through external fastener between connecting hole A6 and the connecting hole B17, thereby can dismantle the connection through rotating external fastener along connecting hole A6 and connecting hole B17 between connecting hole A6 and the connecting hole B17, and then just also can dismantle the connection with having carried out between limiting plate 5 and the curb plate 16, just so accomplished the sealed operation of culture tank 2 and waste liquid groove 4.

Specifically, 1 right side outer wall of box body is provided with assists fagging 7, just assist fagging 7 and limiting plate 5's horizontal width the same, make box body 1 can the level place at the holding surface upper surface through assisting fagging 7 and limiting plate 5's horizontal width the same.

Specifically, the volume of the culture tank 2 is larger than that of the waste liquid tank 4, and the culture tank 2 can exert its function of designing cultured cells as much as possible by making the volume of the culture tank 2 larger than that of the waste liquid tank 4.

When in use, firstly, the outer wall of the bottom surface of the sealing gasket 15 can be clamped along the opening ends of the culture tank 2 and the waste liquid tank 4, and the outer wall of the bottom surface of the cover plate 14 is attached to the upper surface of the box body 1, meanwhile, the side plate 16 can be inserted into the middle position of the limiting plate 5, then, the external fasteners can be rotated along the connecting hole A6 and the connecting hole B17, so that the connecting hole A6 and the connecting hole B17 can be detachably connected, further, the limiting plate 5 and the side plate 16 can be detachably connected, thus, the sealing operation of the culture tank 2 and the waste liquid tank 4 is completed, the culture tank is effectively convenient to disassemble and assemble, the inside of the culture tank 2 and the waste liquid tank 4 is convenient to regularly clean and sterilize, then, the upper surface of the cover plate 14 of the culture tank is attached to the upper surface of the supporting surface, and a culture medium and cell mixture can be added into the culture tank 2 through the culture pipe 11, then the culture tube 11 can be sealed by rotating the screw cap 13 along the external thread A12, thus air can freely enter the culture tank 2 along the culture tube 11 to perform cell culture operation under the action of the air permeable membrane 18 arranged on the inner wall of the bottom surface of the screw cap 13, when the culture medium in the culture tank 2 needs to be replaced, at this time, the solution for medium permeation can be added into the waste liquid tank 4 along the waste liquid pipe 8, then the lower end of the waste liquid pipe 8 can be sealed by rotating the sealing cap 10 along the external thread B9, then the box body 1 can be horizontally arranged along the limiting plate 5 and the auxiliary supporting plate 7, meanwhile, the culture medium waste liquid can be permeated into the waste liquid tank 4 under the action of the filter membrane 19 arranged on the outer side wall of the clapboard 3, then the waste liquid in the waste liquid tank 4 can be discharged by rotating the sealing cap 10 and the waste liquid pipe 8, and the function of cell culture can be effectively realized, and can in time change the operation to the culture medium when the culture medium needs to be changed, need not to adopt centrifugal mode to get rid of the culture medium, avoided getting rid of the damage that the culture medium in-process takes place the cell, this culture box simple structure and practicality are stronger.

The components are all standard parts or parts known to the person skilled in the art, the structure and the principle of which are known to the person skilled in the art from technical manuals or by routine experimentation.

It should be noted that, in this document, relational terms such as first and second, and the like are used solely to distinguish one entity or action from another entity or action without necessarily requiring or implying any actual such relationship or order between such entities or actions. Also, the terms "comprises," "comprising," or any other variation thereof, are intended to cover a non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements does not include only those elements but may include other elements not expressly listed or inherent to such process, method, article, or apparatus.

Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.

Claims

1. A cell culture box convenient to change culture medium, includes box body (1), its characterized in that: the utility model discloses a waste liquid tank, including box body (1), culture tank (2) and waste liquid groove (4), the ascending culture tank of open end (2) and waste liquid groove (4) upper surface are seted up, just culture tank (2) and waste liquid groove (4) intermediate position are provided with baffle (3), it has the one end of culture tube (11) to peg graft in culture tank (2) bottom surface inner wall, just the other end of culture tube (11) extends to box body (1) bottom surface outer wall and has seted up external screw thread A (12), external screw thread A (12) threaded connection has screw cap (13), waste liquid groove (4) bottom surface inner wall is pegged graft and is had the one end of waste liquid pipe (8), just the other end of waste liquid pipe (8) extends to box body (1) bottom surface outer wall and has seted up external screw thread B (9), external screw thread B (9) threaded connection has sealing cap (10), culture tank (2) and waste liquid groove (4) outer wall block are connected with apron (14).

2. A cell culture cassette for facilitating media exchange according to claim 1, wherein: the outer side wall of the cover plate (14) is provided with a side plate (16), a connecting hole B (17) is formed in the outer side wall of the side plate (16), and a sealing gasket (15) is arranged on the outer wall of the bottom surface of the cover plate (14).

3. A cell culture cassette for facilitating media exchange according to claim 1, wherein: the outer side wall of the partition board (3) is provided with a filter membrane (19), the aperture of the filter membrane (19) is 1-4 microns, and the distance between the filter membrane (19) and the inner wall of the bottom surface of the culture tank (2) is smaller than the distance between the filter membrane (19) and the upper surface of the box body (1).

4. A cell culture cassette for facilitating media exchange according to claim 2, wherein: the outer side wall of the box body (1) is provided with a limiting plate (5), and the outer side wall of the limiting plate (5) is provided with a connecting hole A (6).

5. A cell culture cassette for facilitating media exchange according to claim 1, wherein: the inner wall of the bottom surface of the screw cap (13) is provided with a breathable film (18), and the aperture of the breathable film (18) is 0.45 micrometer.

6. A cell culture cassette for facilitating media exchange according to claim 4, wherein: the number of limiting plate (5) is two, just curb plate (16) are located limiting plate (5) intermediate position, can dismantle the connection through external fastener between connecting hole A (6) and connecting hole B (17).

7. A cell culture cassette for facilitating media exchange according to claim 4, wherein: the outer wall of the right side of the box body (1) is provided with an auxiliary supporting plate (7), and the auxiliary supporting plate (7) and the limiting plate (5) are the same in transverse width.

8. A cell culture cassette for facilitating media exchange according to claim 1, wherein: the volume of the culture tank (2) is larger than that of the waste liquid tank (4).