CN218478762U - A supplementary temperature control device for nucleic acid amplification - Google Patents
A supplementary temperature control device for nucleic acid amplification Download PDFInfo
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- CN218478762U CN218478762U CN202222411221.0U CN202222411221U CN218478762U CN 218478762 U CN218478762 U CN 218478762U CN 202222411221 U CN202222411221 U CN 202222411221U CN 218478762 U CN218478762 U CN 218478762U
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Abstract
The utility model discloses an auxiliary temperature control device for nucleic acid amplification, including accuse temperature case, cold water storage cistern and hot-water tank, accuse temperature case fixed mounting is on cold water storage cistern and hot-water tank top, and accuse temperature bottom fixed mounting has driving motor, and driving motor sets up between cold water storage cistern and hot-water tank, and fixed mounting has the device inner box in the accuse temperature case, constitutes the temperature changing chamber between accuse temperature case and the device inner box, and the axis of rotation is installed in the rotation of accuse temperature incasement bottom, and the axis of rotation runs through accuse temperature incasement bottom and device inner box bottom. The utility model discloses a set up cold water circulation mechanism and hot water circulation mechanism, the hot water pumping after the second suction pump will heat through electric heating pipe when needs heat is delivered to the alternating temperature intracavity, blows the heat that the fan leaf produced the hot water and blows to device inner box upper end, guarantees the homogeneity to the test tube heating, and when needs are cooled down, first suction pump is delivered cold water pumping to the alternating temperature intracavity, and stirring paddle leaf is with the water misce bene.
Description
Technical Field
The utility model relates to a gene detection equipment field especially relates to an auxiliary temperature control device for nucleic acid amplification.
Background
At present, the PCR amplification device is used for detecting target nucleic acid molecules, so that the absolute quantitative precision and the dynamic range of DNA can be very high, and the cost and the operation time are greatly reduced. At present, a PCR amplification device discretizes nucleic acid molecules on a micropore array chip board to realize digital amplification, but the existing PCR amplification device lacks an effective temperature control device, cannot control temperature change in a sample amplification process, and has poor temperature uniformity of the nucleic acid molecules at different positions, so that the amplification efficiency is inconsistent.
In view of the above problems, publication No.: CN210261762U discloses a temperature control device for a PCR amplification device, which comprises a thermal cycler, wherein the thermal cycler comprises a base and an upper cover movably mounted on the top of the base. The upper end of base is equipped with the chamber of placing that is used for holding micropore array chip board, the below of placing the chamber is equipped with and is used for adjusting place the temperature control assembly of chamber temperature. The temperature control assembly comprises a heat conduction block which is vertically arranged and is abutted against the bottom of the placing cavity. The upper end of the heat conduction block is provided with a temperature sensor for detecting the temperature of the placing cavity, and the lower end of the heat conduction block is provided with a heater and a radiator which are placed up and down. The utility model discloses can the effective control nucleic acid molecule amplify the temperature, guarantee that being heated of micropore array chip board is even, improve the uniformity of nucleic acid molecule amplification efficiency.
However, in the using process of the device, heat dissipation is mainly performed after heating, the heat dissipation is slow, amplification is influenced, and meanwhile, the heating mode of the device is not uniform enough, so that the amplification conditions of nucleic acids in different test tubes are different. Therefore, it is necessary to provide an auxiliary temperature control device for nucleic acid amplification.
SUMMERY OF THE UTILITY MODEL
The utility model aims at solving the defects in the prior art and providing an auxiliary temperature control device for nucleic acid amplification.
In order to realize the purpose, the utility model adopts the following technical scheme:
the utility model provides an auxiliary temperature control device for nucleic acid amplification, includes accuse temperature case, cold water storage cistern and hot-water tank, accuse temperature case fixed mounting is on cold water storage cistern and hot-water tank top, accuse temperature bottom fixed mounting has driving motor, just driving motor sets up between cold water storage cistern and hot-water tank, fixed mounting has the device inner box in the accuse temperature case, constitute the temperature-variable chamber between accuse temperature case and the device inner box, the axis of rotation is installed in the rotation of accuse temperature incasement bottom, just the axis of rotation runs through accuse temperature incasement bottom and device inner box bottom, the coaxial fixed connection of axis of rotation and driving motor output shaft, fixed mounting has stirring paddle leaf in the temperature-variable chamber in the axis of rotation, fixed mounting has the fan leaf in the device inner box in the axis of rotation, the cold water storage cistern top is provided with cold water circulation mechanism, the hot-water circulation mechanism is provided with on the hot-water tank top.
Preferably, cold water circulation mechanism includes the first suction pump of fixed mounting on cold water storage cistern top, first suction pump is through first inlet tube and the intercommunication of alternating temperature intracavity top, first inlet tube passes through mounting fixed mounting on the accuse temperature lateral wall, first suction pump leads to pipe and cold water storage cistern bottom intercommunication, the cold water storage cistern passes through first communicating pipe intercommunication with the alternating temperature chamber, fixed mounting has first electromagnetism gate valve on the first communicating pipe.
Preferably, hot water circulation mechanism includes the second suction pump of fixed mounting on the hot-water tank top, the second suction pump passes through second inlet tube and the intracavity top intercommunication of alternating temperature, the second inlet tube passes through mounting fixed mounting on accuse temperature case lateral wall, the second suction pump leads to pipe and hot-water tank bottom intercommunication, the hot-water tank passes through second communicating pipe with the alternating temperature chamber and communicates, fixed mounting has the second electromagnetism gate valve on the second communicating pipe.
Preferably, the inner wall of the inner box of the device is fixedly provided with a fixing bulge, the fixing bulge is provided with a reagent support, the reagent support is provided with a plurality of reagent placing holes, and the reagent support is provided with a plurality of air holes.
Preferably, the cover plates are symmetrically and rotatably arranged on two sides of the top end of the temperature control box, the adjacent ends of the cover plates are provided with bulges and notches which are matched with each other, the top end of the cold water tank is provided with a first water inlet, and the top end of the hot water tank is provided with a second water inlet.
Preferably, the inner bottom end of the hot water tank is fixedly provided with an electric heating pipe, and the outer side wall of the cold water tank is fixedly provided with a control panel.
The utility model discloses following beneficial effect has:
through setting up cold water circulation mechanism and hot water circulation mechanism, the hot water pump after will heating through electric heating pipe is pumped to the alternating temperature intracavity to the second suction pump when needs heat, blows the heat that the fan leaf produced the hot water and blows to device inner box upper end, guarantees the homogeneity to the test tube heating, when needs are cooled down, in will hot water carries backheat water tank, first suction pump pumps cold water to the alternating temperature intracavity, stirring paddle leaf is with water misce bene, the fan leaf of blowing can blow off unnecessary heat from the bleeder vent.
Drawings
FIG. 1 is a schematic structural diagram of an auxiliary temperature control device for nucleic acid amplification according to the present invention;
FIG. 2 is a schematic diagram of an external structure of an auxiliary temperature control device for nucleic acid amplification according to the present invention;
fig. 3 is a schematic diagram of the reagent holder of the present invention.
In the figure: 1. a temperature control box; 2. an apparatus inner box; 3. a temperature-variable cavity; 4. a cover plate; 5. a protrusion; 6. a reagent support; 7. a reagent placement well; 8. air holes are formed; 9. a cold water tank; 10. a first water inlet; 11. a first water pump; 12. a fixing member; 13. a first water inlet pipe; 14. a first communication pipe; 15. a first solenoid gate valve; 16. a drive motor; 17. a rotating shaft; 18. a stirring paddle; 19. a blower blade; 20. a hot water tank; 21. a second water inlet; 22. an electric heating tube; 23. a second water pump; 24. a second water inlet pipe; 25. a second communicating pipe; 26. a second solenoid gate valve; 27. a control panel.
Detailed Description
The technical solutions in the embodiments of the present invention will be described clearly and completely with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments.
In the description of the present invention, it should be understood that the terms "upper", "lower", "front", "rear", "left", "right", "top", "bottom", "inner", "outer", and the like indicate orientations or positional relationships based on orientations or positional relationships shown in the drawings, and are only for convenience of description and simplicity of description, but do not indicate or imply that the device or element referred to must have a particular orientation, be constructed and operated in a particular orientation, and therefore, should not be construed as limiting the present invention.
Referring to fig. 1-3, an auxiliary temperature control device for nucleic acid amplification comprises a temperature control box 1, a cold water tank 9 and a hot water tank 20, wherein the temperature control box 1 is fixedly installed at the top ends of the cold water tank 9 and the hot water tank 20, a driving motor 16 is fixedly installed at the bottom end of the temperature control box 1, the driving motor 16 is arranged between the cold water tank 9 and the hot water tank 20, an inner device box 2 is fixedly installed in the temperature control box 1, a temperature-variable cavity 3 is formed between the temperature control box 1 and the inner device box 2, a rotating shaft 17 is rotatably installed at the bottom end of the temperature control box 1 and penetrates through the bottom end of the inner device box 2, the rotating shaft 17 is coaxially and fixedly connected with an output shaft of the driving motor 16, a stirring paddle blade 18 is fixedly installed on the rotating shaft 17 in the temperature-variable cavity 3, a blowing fan blade 19 is fixedly installed on the rotating shaft 17 in the inner device box 2, a cold water circulation mechanism is arranged at the top end of the cold water tank 9, and a hot water circulation mechanism is arranged at the top end of the hot water tank 20.
The cold water circulating mechanism comprises a first water suction pump 11 fixedly installed at the top end of the cold water tank 9, the first water suction pump 11 is communicated with the top end of the temperature changing cavity 3 through a first water inlet pipe 13, the first water inlet pipe 13 is fixedly installed on the outer side wall of the temperature control box 1 through a fixing piece 12, the first water suction pump 11 is communicated with the bottom end of the cold water tank 9 through a water pipe, the cold water tank 9 is communicated with the temperature changing cavity 3 through a first communicating pipe 14, and a first electromagnetic gate valve 15 is fixedly installed on the first communicating pipe 14.
The hot water circulation mechanism comprises a second water suction pump 23 fixedly installed at the top end of the hot water tank 20, the second water suction pump 23 is communicated with the top end of the variable temperature cavity 3 through a second water inlet pipe 24, the second water inlet pipe 24 is fixedly installed on the outer side wall of the temperature control box 1 through a fixing piece 12, the second water suction pump 23 is communicated with the bottom end of the hot water tank 20 through a water pipe, the hot water tank 20 is communicated with the variable temperature cavity 3 through a second communicating pipe 25, and a second electromagnetic gate valve 26 is fixedly installed on the second communicating pipe 25.
The device 2 inner wall fixed mounting of inner box has fixed protrusion 5, has placed reagent on the fixed protrusion 5 and has held in the palm 6, has seted up a plurality of reagents on holding in the palm 6 and has placed hole 7, has seted up a plurality of bleeder vents 8 on the reagent holds in the palm 6.
The cover plates 4 are symmetrically and rotatably arranged on two sides of the top end of the temperature control box 1, the adjacent ends of the cover plates 4 are provided with matched bulges and notches, the top end of the cold water tank 9 is provided with a first water inlet 10, and the top end of the hot water tank 20 is provided with a second water inlet 21.
The electric heating pipe 22 is fixedly installed at the inner bottom end of the hot water tank 20, the control panel 27 is fixedly installed on the outer side wall of the cold water tank 9, and the control panel 27 is electrically connected with the first water suction pump 11, the first electromagnetic gate valve 15, the driving motor 16, the electric heating pipe 22, the second water suction pump 23 and the second electromagnetic gate valve 26.
It should be noted that, the electric control technology of the control panel 27 is a mature technology in the prior art, and is not the technical point of the present invention, and is not described herein again.
The utility model discloses in, place the test tube on reagent holds in the palm reagent on 6 places hole 7, close apron 4, pour into cold water into in cold water tank 9 and the hot-water tank 20 through first water inlet 10 and second water inlet 21, open electric heating pipe 22 through control panel 27, treat to heat suitable temperature with the hot-water tank 20 internal water, start second suction pump 23 and pass through water pipe and second inlet tube 24 and carry to the alternating temperature chamber 3 in, start driving motor 16, make blower fan leaf 19 rotate, carry the heat of bottom to the device inner box 2 upper end in the temperature control box 1, make the heating to reagent more even, when needs cool down, start second electromagnetic gate valve 26, carry the hot water in the alternating temperature chamber 3 back to the hot water tank 20 through second communicating pipe 25 in, close second electromagnetic gate valve 26, start first suction pump 11, carry the cold water in the cold water tank 9 to the alternating temperature chamber 3 through water pipe and first inlet tube 13, blower fan leaf 19 rotates and blows off the heat in the device inner box 2 from alternating temperature chamber 8 bleeder vent.
The above, only be the concrete implementation of the preferred embodiment of the present invention, but the protection scope of the present invention is not limited thereto, and any person skilled in the art is in the technical scope of the present invention, according to the technical solution of the present invention and the utility model, the concept of which is equivalent to replace or change, should be covered within the protection scope of the present invention.
Claims (6)
1. The utility model provides an supplementary temperature control device for nucleic acid amplification, includes accuse temperature case (1), cold water tank (9) and hot-water tank (20), its characterized in that, accuse temperature case (1) fixed mounting is on cold water tank (9) and hot-water tank (20) top, accuse temperature case (1) bottom fixed mounting has driving motor (16), just driving motor (16) set up between cold water tank (9) and hot-water tank (20), fixed mounting has device inner box (2) in accuse temperature case (1), constitute between accuse temperature case (1) and the device inner box (2) and become warm chamber (3), axis of rotation (17) is installed in accuse temperature case (1) bottom rotation, just axis of rotation (17) runs through in accuse temperature case (1) bottom and device inner box (2) bottom, axis of rotation (17) and the coaxial fixed connection of driving motor (16) output shaft, change warm chamber (3) inherent fixed mounting have stirring paddle (18) on axis of rotation (17), the device inner box (2) inherent fixed mounting on axis of rotation (17) is provided with hot-water tank (20) circulation fan mechanism top, cold water tank (9) top is provided with cold water circulation mechanism (20).
2. The auxiliary temperature control device for nucleic acid amplification according to claim 1, wherein the cold water circulation mechanism comprises a first water pump (11) fixedly installed at the top end of a cold water tank (9), the first water pump (11) is communicated with the inner top end of the temperature-changing chamber (3) through a first water inlet pipe (13), the first water inlet pipe (13) is fixedly installed on the outer side wall of the temperature-controlling tank (1) through a fixing member (12), the first water pump (11) is communicated with the bottom end of the cold water tank (9) through a water pipe, the cold water tank (9) is communicated with the temperature-changing chamber (3) through a first communication pipe (14), and a first electromagnetic gate valve (15) is fixedly installed on the first communication pipe (14).
3. The auxiliary temperature control device for nucleic acid amplification according to claim 1, wherein the hot water circulation mechanism comprises a second water pump (23) fixedly installed at the top end of a hot water tank (20), the second water pump (23) is communicated with the inner top end of the temperature-changing chamber (3) through a second water inlet pipe (24), the second water inlet pipe (24) is fixedly installed on the outer side wall of the temperature-controlling tank (1) through a fixing member (12), the second water pump (23) is communicated with the bottom end of the hot water tank (20) through a water pipe, the hot water tank (20) is communicated with the temperature-changing chamber (3) through a second communicating pipe (25), and a second electromagnetic gate valve (26) is fixedly installed on the second communicating pipe (25).
4. The auxiliary temperature control device for nucleic acid amplification according to claim 1, wherein a fixing protrusion (5) is fixedly installed on the inner wall of the device inner box (2), a reagent holder (6) is placed on the fixing protrusion (5), a plurality of reagent placing holes (7) are formed in the reagent holder (6), and a plurality of air holes (8) are formed in the reagent holder (6).
5. The auxiliary temperature control device for nucleic acid amplification according to claim 1, wherein the cover plates (4) are symmetrically and rotatably mounted on two sides of the top end of the temperature control box (1), the adjacent ends of the cover plates (4) are provided with matched bulges and notches, the top end of the cold water tank (9) is provided with a first water inlet (10), and the top end of the hot water tank (20) is provided with a second water inlet (21).
6. The auxiliary temperature control device for nucleic acid amplification according to claim 1, wherein the electric heating tube (22) is fixedly installed at the inner bottom end of the hot water tank (20), and the control panel (27) is fixedly installed at the outer side wall of the cold water tank (9).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202222411221.0U CN218478762U (en) | 2022-09-13 | 2022-09-13 | A supplementary temperature control device for nucleic acid amplification |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202222411221.0U CN218478762U (en) | 2022-09-13 | 2022-09-13 | A supplementary temperature control device for nucleic acid amplification |
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| CN218478762U true CN218478762U (en) | 2023-02-14 |
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| CN202222411221.0U Active CN218478762U (en) | 2022-09-13 | 2022-09-13 | A supplementary temperature control device for nucleic acid amplification |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN117143723A (en) * | 2023-09-05 | 2023-12-01 | 江苏吉诺思美精准医学科技有限公司 | PCR detection device and method |
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2022
- 2022-09-13 CN CN202222411221.0U patent/CN218478762U/en active Active
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN117143723A (en) * | 2023-09-05 | 2023-12-01 | 江苏吉诺思美精准医学科技有限公司 | PCR detection device and method |
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