CN218470746U - Microfluidic analysis kit - Google Patents

Abstract

The utility model relates to a microfluidic analysis kit, which comprises a cover plate, a reaction block and a base block; the reaction block is provided with a reaction cavity and a plurality of liquid storage cavities, a micro-channel is arranged between the reaction cavity and the liquid storage cavities, and the liquid storage cavities are used for storing reagents to be filled into the reaction cavity. The base block is overlapped below the reaction block, the upper surface of the base block can be attached to the lower surface of the reaction block and sealed with the lower surface of the reaction block, a driving assembly is arranged on the base block, and the driving assembly can convey reagents in the liquid storage cavity to the reaction cavity. The cover plate is superposed above the reaction block, the lower surface of the cover plate can be attached to the upper surface of the reaction block and sealed with each other, and the cover plate is provided with a sample adding hole. The modular application of the kit reduces the manufacturing and using cost. All reagents are sealed alone and are preloaded in the liquid storage cavity, and the pressure that improves the liquid storage cavity through drive assembly during the use releases liquid, has effectively improved the antipollution ability of reagent, prevents false negative, improves the repeatability and the accurate nature of detection.

Description

Microfluidic analysis kit

Technical Field

The utility model relates to a micro-fluidic luminous immunodetection technical field especially relates to a micro-fluidic analysis kit.

Background

Currently, there are two main trends of in vitro diagnostic and analytical instruments, one is large laboratory instruments and equipment with integration, automation, high sensitivity and high throughput; the other type is a miniaturized, portable and rapid analysis instrument which can be used for rapid diagnosis on site. According to the requirement of the grading diagnosis, the measurement of the small hospital and the detection center is small, the small hospital and the detection center are not suitable for purchasing expensive large-scale automatic detection equipment, and the small and rapid detection mode is more suitable for the small hospital. Meanwhile, for some special situations, the scene of detection needs to be realized rapidly at the bedside, and the method is more suitable for adopting small-sized equipment. The micro-fluidic chip can integrate basic operation units such as sample preparation, reaction, separation, detection and the like related in the fields of biology, chemistry, medicine and the like on a chip with a micro-scale micro-channel, and then the micro-fluidic chip is placed in detection equipment for reaction and luminescence detection, so that the whole process of reaction and analysis is automatically completed, and a miniaturized and rapid detection mode is met. The micro-fluidic chip has the advantages of less sample consumption, high integration degree of reagents and high analysis speed, and is very suitable for being made into portable rapid detection equipment for field analysis. The detection has high requirement on time, but the existing microfluidic chip has the problems of insufficient reagent reaction, small detection amount, high requirement on the precision of a sensor of an instrument and the like in the using process.

SUMMERY OF THE UTILITY MODEL

Technical problem to be solved

In view of the above-mentioned shortcoming and the inadequacy of prior art, the utility model provides a microfluidic analysis kit, it has solved the technical problem that current microfluidic chip reaction is insufficient, the uniformity is poor, high and control complicacy to the sensor precision requirement of instrument.

(II) technical scheme

In order to achieve the above object, the microfluidic assay kit of the present invention comprises:

a cover plate, a reaction block and a base block;

the reaction block is provided with a reaction cavity and a plurality of liquid storage cavities, a microchannel is arranged between the reaction cavity and each liquid storage cavity, and the liquid storage cavities are used for storing reagents to be filled into the reaction cavity;

the base block is superposed below the reaction block, the upper surface of the base block can be attached to the lower surface of the reaction block and sealed with each other, a driving assembly is arranged on the base block, and the driving assembly can convey the reagent in the liquid storage cavity into the reaction cavity;

the cover plate is arranged above the reaction block in an overlapping mode, the lower surface of the cover plate can be attached to the upper surface of the reaction block and sealed with the upper surface of the reaction block, and sample adding holes are formed in the cover plate and located right above the reaction cavity.

Optionally, the liquid storage cavity penetrates through the upper surface and the lower surface of the reaction block, and an upper end opening of the liquid storage cavity is communicated with an upper end opening of the reaction cavity through the microchannel.

Optionally, the driving assembly includes a plurality of first plungers, a first through hole has all been seted up to the position that corresponds each stock solution chamber on the base block, and is a plurality of first plungers are respectively with a plurality of first through hole one-to-one cover is established, first plunger can be followed the lower surface of reaction piece inserts in the stock solution chamber.

Optionally, a cleaning cavity is further formed in the reaction block and used for storing cleaning liquid;

the cleaning cavity penetrates through the upper surface and the lower surface of the reaction block, and an upper end opening of the cleaning cavity is communicated with an upper end opening of the reaction cavity;

the driving assembly further comprises a second plunger, a second through hole is formed in the position, corresponding to the cleaning cavity, of the base block, the second plunger is sleeved in the second through hole, and the second plunger can be inserted into the cleaning cavity from the lower surface of the reaction block.

Optionally, the reaction block is further provided with a waste liquid cavity, the waste liquid cavity penetrates through the upper surface and the lower surface of the reaction block, and an opening at the upper end of the waste liquid cavity is communicated with an opening at the lower end of the reaction cavity;

the driving assembly further comprises a third plunger, a third through hole is formed in the position, corresponding to the waste liquid cavity, of the base block, the third plunger is sleeved in the third through hole, and the third plunger can be inserted into the waste liquid cavity from the lower surface of the reaction block.

Optionally, at least one microchannel is disposed between each liquid storage cavity and the reaction cavity.

Optionally, the cross section of the microchannel is circular, and the inner diameter of the microchannel is um to um.

Optionally, the microfluidic assay kit further comprises a heating assembly, wherein a plurality of arc-shaped grooves are formed in the lower surface of the reaction block, the plurality of arc-shaped grooves are arranged around the reaction chamber, and the heating assembly is arranged in the arc-shaped grooves.

Optionally, a plurality of the liquid storage cavities are arranged around the reaction cavity in an annular array.

Optionally, the cover plate and the reaction block are both made of transparent materials.

(III) advantageous effects

The modular application of the kit reduces the manufacturing and using cost. All reagents are sealed and preassembled in the liquid storage cavity independently, and liquid is released by improving the pressure of the liquid storage cavity through the driving assembly during use, so that the pollution resistance of the reagents is effectively improved, false negative is prevented, and the repeatability and accuracy of detection are improved. Through differential control of the flow path pressure of the reaction area, the full mixing and reaction of the fluid can be effectively accelerated, and the generation of bubbles can be effectively reduced, so that the detection accuracy and repeatability are improved.

Drawings

Fig. 1 is an exploded view of a microfluidic assay kit according to the present invention;

FIG. 2 is a top view of a reaction block of the microfluidic assay kit of the present invention;

FIG. 3 is a schematic view of a part of the structure of the microfluidic assay kit according to the present invention;

fig. 4 is a top view of a base block of the microfluidic assay kit of the present invention.

[ description of reference ]

1: a cover sheet; 11: a sample application hole;

2: a reaction block; 21: a reaction chamber; 22: a liquid storage cavity; 23: a microchannel; 24: a cleaning chamber; 25: a waste fluid chamber; 26: an arc-shaped slot;

3: a base block; 31: a first plunger; 32: a second plunger; 33: a third plunger; 34: and installing the through hole.

Detailed Description

For a better understanding of the present invention, reference will now be made in detail to the present invention, examples of which are illustrated in the accompanying drawings. In which the terms "upper", "lower", etc. are used herein with reference to the orientation of fig. 1.

While exemplary embodiments of the invention are shown in the drawings, it should be understood that the invention can be embodied in various forms and should not be limited to the embodiments set forth herein. Rather, these embodiments are provided so that this disclosure will be thorough and complete, and will fully convey the scope of the invention to those skilled in the art.

As shown in figures 1 and 2, the utility model provides a microfluidic assay kit, it includes cover plate 1, reaction block 2 and base block 3 of superpose in proper order, through the modular application of kit, reduces preparation and use cost. Reaction chamber 21 and a plurality of stock solution chamber 22 have been seted up on reaction block 2, a plurality of stock solution chamber 22 annular array set up around reaction chamber 21, all be provided with microchannel 23 between each stock solution chamber 22 and the reaction chamber 21, reaction chamber 21 is used for biochemical reaction, stock solution chamber 22 is used for storing the reagent that waits to be annotated, the reagent includes luminescent reagent, liquid enzyme labeling ligand and magnetic bead labeling ligand, wherein, luminescent reagent can be luminol and its derivative etc. liquid enzyme labeling ligand can be horseradish peroxidase etc. magnetic bead labeling ligand is conventional general type reagent, do not specifically limit here. Through the liquid storage cavity 22 that the annular array set up and the pipeline design that adopts microchannel 23 to connect for the kit size is littleer, and the universalization degree is high. After the reaction block 2 is installed on the base block 3, the upper surface of the base block 3 can be attached to the lower surface of the reaction block 2, and the two surfaces are sealed with each other. Correspond each stock solution chamber 22 on the base block 3 and all be provided with drive assembly, drive assembly carries out mixing reaction through the reagent propelling movement in the pressurized mode in with stock solution chamber 22 to reaction chamber 21. The cover plate 1 is superposed above the reaction block 2, and after the cover surface 1 is arranged on the reaction block 2, the lower surface of the cover plate 1 can be attached to the upper surface of the reaction block 2 and the two surfaces are mutually sealed. The cover plate 1 is provided with a sample adding hole 11, after the cover plate 1 is positioned and installed, the sample adding hole 11 is positioned right above the reaction cavity 21, and a sample is dripped into the reaction cavity 21 through the sample adding hole 11. The well 11 is provided with a sealing tape for covering the well 11 to completely seal the reaction chamber 21, and the sealing member is preferably a sealing tape. When in use, the reaction block 2 is firstly stacked on the base block 3, then the reagent to be used and the luminescent reagent are added into the liquid storage cavity 22 according to the measurement required by the reaction, finally the cover plate 1 is stacked on the reaction block 2, and the liquid storage cavity 22 and the reaction cavity 21 are clamped by the base block 3 and the cover plate 1 to form a completely sealed cavity; all reagents are sealed and preassembled in the liquid storage cavity 22 independently, and liquid is released by improving the pressure of the liquid storage cavity 22 through the driving assembly during use, so that the pollution resistance of the reagents is effectively improved, false negative is prevented, and the repeatability and the accuracy of detection are improved. Through differential control of the flow path pressure of the reaction area, the full mixing and reaction of the fluid can be effectively accelerated, and the generation of bubbles can be effectively reduced, so that the detection accuracy and repeatability are improved.

The utility model discloses a drive assembly changes the speed and the stroke of reagent filling, and the pressure change in the control reaction chamber 21 has promoted the motion and the mixture of liquid effectively to it is more abundant to make the reaction. The reagent is quantitatively placed in the liquid storage cavity 22 in advance, so that the use amount of the reaction sample and the reagent is reduced, the precision of the liquid reaction temperature is conveniently controlled, and the sensitivity is effectively improved. By pre-packaging the reagent with accurate quantification and controlling the liquid flux of the micro-channel 23, the accuracy of the reaction reagent amount is improved. The operator only needs to add the detection sample, seal the operation to put the reagent box after sealing into miniaturized detecting instrument, detect and independently accomplish in the reagent box, improved the convenient degree of operation.

As shown in fig. 2 and fig. 3, a plurality of circular through holes arranged around the reaction cavity 21 in an annular array are arranged on the reaction block 2, the circular through holes are clamped by the base block 3 and the cover plate 1 to form a completely sealed cavity, namely, the liquid storage cavity 22 penetrates through the upper surface and the lower surface of the reaction block 2, and the upper end opening of the liquid storage cavity 22 is communicated with the upper end opening of the reaction cavity 21 through the micro-channel 23. When the driving assembly pressurizes the reservoir chamber 22, the reagent in the reservoir chamber 22 flows into the reaction chamber 21 through the micro channel 23. Specifically, the driving assembly comprises a plurality of first plungers 31, a first through hole is formed in the base block 3 corresponding to each liquid storage cavity 22, and the first plungers 31 are sleeved with the first through holes in a one-to-one correspondence mode. After installing reaction block 2 location on base block 3, first through-hole and stock solution chamber 22's position phase-match, first plunger 31 is in initial condition, initial condition's first plunger 31's up end and reaction block 2's lower surface coplane, can insert stock solution chamber 22 with first plunger 31 from reaction block 2's lower surface through the first plunger 31 of propelling movement, and continue to move to reaction block 2's upper surface along stock solution chamber 22's extending direction, thereby improve stock solution chamber 22's atmospheric pressure, and then in the complete propelling movement of the reagent in the stock solution chamber 22 to reaction chamber 21. The utility model discloses can set up a plurality of stock solution chambeies 22 according to the required reagent kind of reaction to the correspondence sets up a plurality of first plungers 31. When reagent is input, the reagent is driven to move by repeatedly pushing and pulling the first plunger piston 31, the stirring function of the sample and the reagent is realized, the mixing efficiency and the reaction efficiency between the sample and the reagent can be effectively improved, and bubbles can be effectively reduced through high-pressure injection, so that the accuracy and the repeatability of detection are improved.

As shown in fig. 2 and fig. 3, a cleaning chamber 24 is further formed on the reaction block 2, and the cleaning chamber 24 is used for storing a cleaning solution. The cleaning cavity 24 penetrates through the upper surface and the lower surface of the reaction block 2, and the upper end opening of the cleaning cavity 24 is communicated with the upper end opening of the reaction cavity 21. The driving assembly further comprises a second plunger 32, a second through hole is formed in the position, corresponding to the cleaning cavity 24, of the base block 3, the second plunger 32 is sleeved in the second through hole, and the second plunger 32 can be inserted into the cleaning cavity 24 from the lower surface of the reaction block 2. The structure of purge chamber 24 is the same as that of reservoir chamber 22, and the initial state of second plunger 32 is the same as that of first plunger 31, and will not be described again.

As shown in fig. 2 and 3, the reaction block 2 is further provided with a waste liquid cavity 25, and the waste liquid cavity 25 is used for collecting waste liquid after detection, so that the waste liquid is collected in a centralized manner, and the convenience in use is improved. The waste liquid cavity 25 penetrates the upper and lower surfaces of the reaction block 2, and an upper end opening of the waste liquid cavity 25 is communicated with a lower end opening of the reaction cavity 21. The driving assembly further comprises a third plunger 33, a third through hole is formed in the position, corresponding to the waste liquid cavity 25, of the base block 3, the third plunger 33 is sleeved in the third through hole, and the third plunger 33 can be inserted into the waste liquid cavity 25 from the lower surface of the reaction block 2. After the reaction block 2 is stacked on the base block 3, the third plunger 33 is in an initial state, and in the initial state, the third plunger 33 is sleeved in the waste liquid cavity 25, the upper end surface of the third plunger 33 is coplanar with the upper surface of the reaction block 2, after detection is finished, the third plunger 33 is pulled downwards, and waste liquid in the reaction cavity 21 is pumped into the waste liquid cavity 25 through negative pressure. The first plunger 31, the second plunger 32 and the third plunger 33 are plunger rods with elastic sealing heads, and the elastic sealing heads are connected with the inner wall of the cavity and the inner wall of the through hole in a sealing and sliding manner.

Preferably, at least one micro-channel 23 is arranged between each liquid storage cavity 22 and the reaction cavity 21, the cross section of the micro-channel 23 is circular, and the inner diameter of the micro-channel 23 is 20 um-100 um.

Preferably, the microfluidic assay kit further comprises a heating assembly for heating the reaction chamber 21. Referring to fig. 3 and 4, a plurality of arc-shaped grooves 26 are formed in the lower surface of the reaction block 2, the arc-shaped grooves 26 are arranged around the reaction chamber 21, the heating assembly is arranged in the arc-shaped grooves 26 and is conventional equipment such as heating wires and heating patches, the heating assembly is close to the reaction chamber 21 as much as possible, and the heating efficiency is effectively improved. Meanwhile, due to the fact that the using amount of the reaction sample and the reagent is reduced, the accuracy of controlling the liquid reaction temperature can be controlled more conveniently due to the improvement of the heating efficiency, and therefore the sensitivity is effectively improved. The base block 3 is also provided with an installation through hole 34 matched with the arc-shaped groove 26, and the installation through hole 34 penetrates through the upper surface and the lower surface of the base block 3 so as to be convenient for installing the heating assembly. Specifically, the heating component is installed in the detection instrument, when the microfluidic assay kit is placed in the detection instrument, the heating component penetrates through the installation through hole 34 and then is sleeved in the arc-shaped groove 26, and the heating temperature is controlled by the detection instrument.

Preferably, the cover plate 1 and the reaction block 2 are made of transparent materials, so that the liquid circulation condition in each cavity can be observed conveniently.

The operation steps are as follows: the reaction block 2 is stacked on the base block 3 with the faces in contact with each other sealed to each other, and the first plunger 31 and the second plunger 32 are pushed to a position where the upper face is coplanar with the lower face of the reaction block 2, and the third plunger 33 is pushed to a position where the upper face is coplanar with the upper face of the reaction block 2. The liquid storage chamber 22 is filled with a predetermined amount of reagent, and the cleaning chamber 24 is filled with a cleaning liquid. The patch 1 was stacked on the reaction block 2 with the surfaces in contact with each other sealed. Opening the sealing member of the sample adding hole 11, adding a sample to be detected into the reaction cavity 21 through the sample adding hole 11, sealing the sample adding hole 11, then putting the microfluidic analysis kit into a detection instrument, installing the heating assembly into the arc-shaped groove 26 in a matching manner, and connecting a driving device in the detection instrument with each plunger in a matching manner. The detection instrument injects the reagent in each liquid storage cavity 22 into the reaction cavity 21 under high pressure according to a preset sequence by controlling the movement of each first plunger 31 to generate a first mixed liquid; high pressure injection can effectively improve mixing efficiency. By repeatedly pushing and pulling the first plunger 31, the liquid in the reaction chamber 21 can move back and forth in the reaction chamber 21 and the liquid storage chamber 22, so that the stirring function of the first mixed liquid is realized, the first mixed liquid is fully mixed and reacted in the reaction chamber 21, and further a second mixed liquid is generated; after the reagent injection is finished, the upper end surface of the first plunger 31 is coplanar with the upper surface of the reaction block 2, so that the second mixed liquid is prevented from being retained in the liquid storage cavity 22. The detection instrument injects the cleaning solution in the cleaning cavity 24 into the reaction cavity 21 by pushing the second plunger 32, so as to clean the second mixed solution; the cleaning liquid can be sufficiently mixed with the second mixed liquid by repeatedly pushing and pulling the second plunger 32. The detecting instrument pushes the first plunger 31 corresponding to the luminescent reagent, inputs the luminescent reagent into the reaction chamber 21, and repeatedly pushes and pulls the first plunger 31, so that the luminescent reagent and the second mixed solution are fully mixed and react to generate a third mixed solution. The detection instrument detects the third mixed solution in the reaction chamber 21. The third plunger 33 is pulled downward to pump the third mixed solution in the reaction chamber 21 to the waste liquid chamber 25. The microfluidic analysis kit is cleaned by splitting the cover plate 1, the reaction block 2 and the base block 3, so that the operation is convenient and fast, and the cleaning is simple.

The utility model discloses a drive assembly changes the speed and the stroke of reagent filling, and the pressure change in the control reaction chamber 21 has promoted the motion and the mixture of liquid effectively to it is more abundant to make the reaction. The reagent is quantitatively placed in the liquid storage cavity 22 in advance, so that the use amount of the reaction sample and the reagent is reduced, the precision of the liquid reaction temperature is conveniently controlled, and the sensitivity is effectively improved. By pre-packaging the reagent with accurate quantification and controlling the liquid flux of the micro-channel 23, the accuracy of the reaction reagent amount is improved. The operating personnel only need add the testing sample, and sealed operation detects independently to accomplish in the reagent box, has improved the convenient degree of operation.

In the description of the present invention, it is to be understood that the terms "first", "second" and "first" are used for descriptive purposes only and are not to be construed as indicating or implying relative importance or to imply that the number of indicated technical features is significant. Thus, a feature defined as "first" or "second" may explicitly or implicitly include one or more of that feature. In the description of the present invention, "a plurality" means two or more unless specifically limited otherwise.

In the present invention, unless otherwise explicitly specified or limited, the terms "mounted," "connected," "secured," and the like are to be construed broadly, e.g., as being fixedly connected, detachably connected, or integrated; can be mechanically or electrically connected; can be directly connected or indirectly connected through an intermediate medium; either as communication within the two elements or as an interactive relationship of the two elements. The specific meaning of the above terms in the present invention can be understood according to specific situations by those skilled in the art.

In the present application, unless otherwise expressly stated or limited, a first feature may be "on" or "under" a second feature, and the first and second features may be in direct contact, or the first and second features may be in indirect contact via an intermediate. Also, a first feature "on," "above," and "over" a second feature may be directly on or obliquely above the second feature, or simply mean that the first feature is at a higher level than the second feature. A first feature being "under," "below," and "beneath" a second feature may be directly under or obliquely under the second feature, or may simply mean that the first feature is at a lower level than the second feature.

In the description of the present specification, the description of "one embodiment", "some embodiments", "examples", "specific examples" or "some examples", etc., means that a particular feature, structure, material, or characteristic described in connection with the embodiment or example is included in at least one embodiment or example of the present invention. In this specification, the schematic representations of the terms used above are not necessarily intended to refer to the same embodiment or example. Furthermore, the particular features, structures, materials, or characteristics described may be combined in any suitable manner in any one or more embodiments or examples. Furthermore, various embodiments or examples and features of different embodiments or examples described in this specification can be combined and combined by one skilled in the art without contradiction.

While embodiments of the present invention have been shown and described, it is to be understood that the above embodiments are exemplary and should not be construed as limiting the present invention, and that modifications, alterations, substitutions and variations may be made to the above embodiments by those of ordinary skill in the art without departing from the scope of the present invention.

Claims (10)

1. A microfluidic assay kit, characterized in that it comprises a cover slip (1), a reaction block (2) and a base block (3);

the reaction block (2) is provided with a reaction cavity (21) and a plurality of liquid storage cavities (22), a microchannel (23) is arranged between the reaction cavity (21) and each liquid storage cavity (22), and the liquid storage cavities (22) are used for storing reagents to be filled into the reaction cavity (21);

the base block (3) is stacked below the reaction block (2), the upper surface of the base block (3) can be attached to the lower surface of the reaction block (2) and sealed with each other, and a driving assembly is arranged on the base block (3) and can convey the reagent in the liquid storage cavity (22) into the reaction cavity (21);

cover plate (1) superpose in the top of reaction piece (2), the lower surface of cover plate (1) can with the upper surface laminating of reaction piece (2) and mutual sealing, sample adding hole (11) have been seted up on cover plate (1), sample adding hole (11) are located directly over reaction chamber (21).

2. The microfluidic assay kit according to claim 1, wherein the reservoir (22) penetrates upper and lower surfaces of the reaction block (2), and an upper opening of the reservoir (22) communicates with an upper opening of the reaction chamber (21) through the microchannel (23).

3. The microfluidic assay kit according to claim 2, wherein the driving assembly comprises a plurality of first plungers (31), a first through hole is formed in the base block (3) at a position corresponding to each of the reservoirs (22), the plurality of first plungers (31) are respectively sleeved with the plurality of first through holes in a one-to-one correspondence manner, and the first plungers (31) can be inserted into the reservoirs (22) from the lower surface of the reaction block (2).

4. The microfluidic assay kit according to any one of claims 1 to 3, wherein the reaction block (2) is further provided with a cleaning chamber (24), and the cleaning chamber (24) is used for storing a cleaning solution;

the cleaning cavity (24) penetrates through the upper surface and the lower surface of the reaction block (2), and an upper end opening of the cleaning cavity (24) is communicated with an upper end opening of the reaction cavity (21);

the driving assembly further comprises a second plunger (32), a second through hole is formed in the position, corresponding to the cleaning cavity (24), of the base block (3), the second plunger (32) is sleeved in the second through hole, and the second plunger (32) can be inserted into the cleaning cavity (24) from the lower surface of the reaction block (2).

5. The microfluidic assay kit according to any one of claims 1 to 3, wherein the reaction block (2) further comprises a waste liquid chamber (25), the waste liquid chamber (25) penetrates through the upper and lower surfaces of the reaction block (2), and an upper opening of the waste liquid chamber (25) is communicated with a lower opening of the reaction chamber (21);

the driving assembly further comprises a third plunger (33), a third through hole is formed in the position, corresponding to the waste liquid cavity (25), of the base block (3), the third plunger (33) is sleeved in the third through hole, and the third plunger (33) can be inserted into the waste liquid cavity (25) from the lower surface of the reaction block (2).

6. Microfluidic assay kit according to any of claims 1 to 3, wherein at least one microchannel (23) is provided between each reservoir (22) and the reaction chamber (21).

7. Microfluidic analytical kit according to claim 6, characterised in that the cross-section of the microchannel (23) is circular and the inner diameter of the microchannel (23) is between 20um and 100um.

8. The microfluidic assay kit according to any of claims 1 to 3, further comprising a heating assembly, wherein the lower surface of the reaction block (2) is provided with a plurality of arc-shaped grooves (26), the plurality of arc-shaped grooves (26) are arranged around the reaction chamber (21), and the heating assembly is arranged in the arc-shaped grooves (26).

9. Microfluidic assay kit according to any of claims 1-3, wherein a plurality of the reservoir chambers (22) are arranged in an annular array around the reaction chamber (21).

10. The microfluidic assay kit according to any of claims 1-3, wherein the cover plate (1) and the reaction block (2) are both made of transparent material.

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