CN218321387U

CN218321387U - Special conical shake flask for culturing PHA (polyhydroxyalkanoate) producing strains

Info

Publication number: CN218321387U
Application number: CN202221299643.7U
Authority: CN
Inventors: 周路; 唐新源; 杨延慧; 牛李杰; 任欢; 张宝新
Original assignee: Yili Chuanning Biotechnology Co ltd
Current assignee: Yili Chuanning Biotechnology Co ltd
Priority date: 2022-05-27
Filing date: 2022-05-27
Publication date: 2023-01-17
Anticipated expiration: 2032-05-27

Abstract

The utility model relates to a microbial cultivation field discloses a special toper bottle that shakes for cultivateing PHA bacterial strain of producing, including the bottle, the bottle is the toper, the bottle lid is connected to the bottleneck department of bottle, and the bleeder vent that link up about the bottle lid top is equipped with the ventilated membrane between bottleneck top and the bottle lid inner wall, the inside wall of bottle is equipped with the baffle, the baffle is linked together with the bottle lateral wall. The utility model discloses simple structure, simple operation, baffle multiplicable shake interior gas-liquid mass transfer ability of bottle have the high efficiency and train the function of producing the PHA bacterial, and the ventilated membrane allows gaseous business turn over bottle, improves the effect that cultivates in-process thallus to oxygen utilization efficiency, enhancement gas-liquid mass transfer greatly, improvement fermentation microenvironment.

Description

Special conical shake flask for culturing PHA (polyhydroxyalkanoate) producing strains

Technical Field

The utility model relates to a microbial cultivation field, concretely relates to a special toper shake bottle for cultivateing and produce PHA bacterial strain.

Background

Polyhydroxyalkanoates (PHA) is a natural biopolymer material, which is a product of bacteria under unbalanced growth conditions, and its physiological function is primarily a storage substance as a carbon source and energy in the body of the bacteria. PHA synthesized by microorganisms has several unique properties, including biodegradability, biocompatibility, piezoelectricity, optical activity, and the like. During the culture of PHA strains, when the growth environment of the strains is lack of certain nutrient substances (such as nitrogen, phosphorus, magnesium and the like) necessary for growth and excessive carbon sources exist, the redox imbalance and the energy and reduction equivalent excess in the bacteria body need to be stored in a substance form to synthesize PHA; obviously, strains capable of accumulating large amounts of PHA are more suitable for the needs of industrial production, and therefore this requires extensive shake flask culture screening of strains in the laboratory. The existing culture shake flask used in a laboratory has the defects of generally simple shake flask result, anoxic shake flask, low gas-liquid mass transfer capacity, small gas-liquid interface area, small specific surface area, small liquid film mass transfer coefficient and the like, so that the culture efficiency of PHA strains is reduced, and the culture requirement of the PHA strains cannot be met.

Chinese patent application No. CN201120355133.2 provides a shake flask for microorganism culture, the shake flask having a baffle on the inner wall, comprising a flask body, and is characterized by further comprising a baffle, one end of the baffle is fixed on the lower part in the flask body and is connected with the flask body into a whole. The shake flask provided by the patent solves the defects of the prior art to a certain extent, but the width D of the baffle is 1/8-1/10 of the average inner diameter of the flask body, the width of the baffle is too narrow, and the surface area of a thin-layer liquid film formed after gas-liquid mass transfer liquid in the shake flask is scratched on the wall surface of the shake flask is too small; the bottle mouth is not provided with a sterile ventilation device, so that the efficiency is improved, excessive oxygen does not enter a shake flask in the earlier stage of shake flask culture, and the thallus can die due to oxygen deficiency.

SUMMERY OF THE UTILITY MODEL

The utility model relates to a special toper bottle that shakes for cultivateing PHA bacterial strain of producing for shake bottle oxygen deficiency, gas-liquid mass transfer ability are lower, gas-liquid interface area is little, liquid film mass transfer coefficient is little scheduling problem among the solution prior art.

In order to realize the technical purpose, the utility model discloses a technical scheme is:

the special conical shake flask for culturing PHA-producing strains comprises a flask body, wherein the flask body is conical, a flask cover is connected to the position of a flask opening of the flask body, air holes which are communicated up and down are formed in the top of the flask cover, a breathable film is arranged between the top of the flask opening and the inner wall of the flask cover, and a baffle is arranged on the inner side wall of the flask body and is connected with the side wall of the flask body into a whole.

Furthermore, the bottle body is made of transparent materials.

Further, the bottle lid is soft cutting ferrule structure, adopts the silica gel material to make.

Further, the bottle mouth thickness is 1.5-2 times of the bottle body thickness, a clamping groove is arranged in the bottle cap, the bottle mouth is clamped in the clamping groove, and the outer wall of the bottle mouth is in interference fit with the clamping groove.

Further, the breathable film is a 0.22 μm sterile hydrophobic film.

Preferably, the height H1 of the bottle body is 1.5-2.0 of the inner diameter D1 of the bottom of the bottle body.

Preferably, the height B1 of the baffle is 1/4-1/6 of the inner diameter D1 of the bottom of the bottle body.

Preferably, the length B2 of the baffle is 1/2-1/3 of the height B1 of the baffle.

Preferably, a gap is reserved between the baffle and the bottle bottom, and the distance H2 between the baffle and the bottle bottom is 1/2-1/4 of the length B2 of the baffle.

Preferably, the number of the baffles is 1-4, and the baffles are uniformly distributed around the circumferential direction of the bottle body.

Compared with the prior art, the beneficial effects of the utility model are that:

the utility model has the advantages of being simple in structure and simple in operation, the multiplicable gas-liquid mass transfer ability of shaking the bottle has the function of high-efficient cultivation production PHA bacterial, still has and improves the effect that cultivates in-process thallus to oxygen utilization efficiency, enhancement gas-liquid mass transfer greatly, improvement fermentation microenvironment. The method can be popularized to flow field analysis of shake flasks of other varieties.

Drawings

In order to more clearly illustrate the technical solutions of the embodiments of the present application, the drawings that are required to be used in the embodiments will be briefly described below, it should be understood that the following drawings only illustrate some embodiments of the present application and therefore should not be considered as limiting the scope, and for those skilled in the art, other related drawings can be obtained from the drawings without inventive effort.

Fig. 1 is a schematic perspective view of an embodiment of the present invention;

FIG. 2 is a cross-sectional view of the embodiment shown in FIG. 1;

fig. 3 is an enlarged view of fig. 2 at the dashed box.

Reference numerals: 1-bottle body, 2-bottle cap, 3-breathable film, 4-bottle mouth and 5-baffle.

Detailed Description

In order to make the objects, technical solutions and advantages of the embodiments of the present application clearer, the technical solutions in the embodiments of the present application will be clearly and completely described below with reference to the drawings in the embodiments of the present application, and it is obvious that the described embodiments are some embodiments of the present application, but not all embodiments. The components of the embodiments of the present application, generally described and illustrated in the figures herein, can be arranged and designed in a wide variety of different configurations. Thus, the following detailed description of the embodiments of the present application, presented in the accompanying drawings, is not intended to limit the scope of the claimed application, but is merely representative of selected embodiments of the application. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present application.

A special tapered shake flask for culturing PHA-producing strains is shown in figures 1 and 2 and comprises a flask body 1, wherein the flask body 1 is tapered, a flask cover 2 is connected to the opening 4 of the flask body 1, air holes which are vertically communicated are formed in the top of the flask cover 2, a breathable film 3 is arranged between the top of the flask opening 4 and the inner wall of the flask cover 2, a baffle 5 is arranged on the inner side wall of the flask body, and the baffle 5 and the side wall of the flask body are connected into a whole.

Preferably, the bottle body 1 is made of transparent materials; the transparent material is one of glass material, PETG material (polyethylene terephthalate-1, 4-cyclohexane dimethanol ester) and polystyrene PS; more preferably, the transparent material is made of glass. Preferably, the bottle cap 2 is of a soft clamping sleeve structure and is made of silica gel. The wall thickness of the bottle mouth is 1.5-2 times of that of the bottle body, a clamping groove is formed in the bottle cap 2, and the bottle mouth is clamped in the clamping groove; the outer wall of the bottle mouth is in interference fit with the clamping groove, as shown in figure 3. Preferably, the breathable film is a 0.22-micron sterile hydrophobic film, and when the bottle mouth is connected with the bottle cap, the breathable film is arranged between the top of the bottle mouth and the top wall of the clamping groove of the bottle cap.

Bottleneck 4 and bottle lid 2 also can adopt threaded connection, and 4 outer walls of bottleneck set up the external screw thread, set up the internal thread in the bottle lid, and the ventilated membrane card is established between bottleneck top and bottle lid inner wall.

Preferably, the height H1 of the bottle body is 1.5-2.0 of the inner diameter D1 of the bottom of the bottle body; the height B1 of the baffle is 1/4-1/6 of the inner diameter D1 of the bottle body; the length B2 of the baffle is 1/2-1/3 of the height B1 of the baffle; a gap is reserved between the baffle and the bottle bottom, and the distance H2 between the baffle and the bottle bottom is 1/2-1/4 of the length B2 of the baffle.

When the bottle is used, the prepared culture medium is quantitatively added into the bottle body 1, the breathable film 3 is covered on the bottle mouth 4, the bottle cap 2 is covered on the breathable film 3 and the bottle mouth 4, and the shake flask is placed in a sterilization chamber for sterilization. After the sterilization is finished, inoculating PHA strains after cooling, certainly, the inoculating process needs to be finished in an aseptic environment, after the inoculation is finished, a shaking bottle is placed on a shaking table for cultivation, when the shaking bottle shakes on a rumor bed, oxygen can enter the bottle body through a breathable film, materials in the shaking bottle cannot generate vortex under the action of a baffle, the gas-liquid mass transfer capacity in the shaking bottle can be increased, the function of efficiently cultivating PHA strains is achieved, and the functions of greatly improving the utilization efficiency of thalli to oxygen in the cultivation process, strengthening the gas-liquid mass transfer and improving the fermentation microenvironment are achieved; if the oxygen demand is low, the permeable membrane can be replaced by an isolation membrane for adjustment.

Of course, the present invention may have other embodiments, and those skilled in the art may make various corresponding changes and modifications according to the present invention without departing from the spirit and the essence of the present invention, and these corresponding changes and modifications should fall within the protection scope of the appended claims.

Claims

1. A special tapered shake flask for culturing PHA-producing strains, which comprises a flask body (1), wherein the flask body (1) is tapered, and is characterized in that: the air-permeable bottle is characterized in that a bottle opening (4) of the bottle body (1) is connected with the bottle cap (2), an air hole which is communicated from top to bottom is formed in the top of the bottle cap (2), an air-permeable film (3) is arranged between the top of the bottle opening (4) and the inner wall of the bottle cap (2), a baffle (5) is arranged on the inner side wall of the bottle body, and the baffle (5) and the side wall of the bottle body are connected into a whole.

2. The specialized conical shake flask for cultivating PHA-producing strains of claim 1, wherein: the bottle body (1) is made of transparent materials.

3. The specialized conical shake flask for the cultivation of PHA-producing strains of claim 1, wherein: bottle lid (2) are soft cutting ferrule structure, adopt the silica gel material to make.

4. The specialized conical shake flask for the cultivation of PHA producing strains of claim 3, wherein: the bottle is characterized in that the thickness of the bottle opening is 1.5-2 times of that of the bottle body, a clamping groove is formed in the bottle cover (2), the bottle opening is clamped in the clamping groove, and the outer wall of the bottle opening is in interference fit with the clamping groove.

5. The specialized conical shake flask for cultivating PHA-producing strains of claim 1, wherein: the breathable film is a 0.22 mu m sterile hydrophobic film.

6. The specialized conical shake flask for cultivating PHA-producing strains of claim 1, wherein: the height H1 of the bottle body is 1.5-2.0 of the inner diameter D1 of the bottom of the bottle body.

7. The specialized conical shake flask for cultivating PHA-producing strains of claim 6, wherein: the height B1 of the baffle is 1/4-1/6 of the inner diameter D1 of the bottom of the bottle body.

8. The specialized conical shake flask for the cultivation of PHA producing strains of claim 7, wherein: the length B2 of the baffle is 1/2-1/3 of the height B1 of the baffle.

9. The specialized conical shake flask for cultivating PHA-producing strains of claim 8, wherein: a gap is reserved between the baffle and the bottle bottom, and the distance H2 between the baffle and the bottle bottom is 1/2-1/4 of the length B2 of the baffle.

10. A specialized conical shake flask for the cultivation of PHA producing strains according to any one of the claims 7 to 9, wherein: the number of the baffle plates is 1-4, and the baffle plates are uniformly distributed around the circumferential direction of the bottle body.