CN218232449U - Culture device and culture system - Google Patents

Abstract

The embodiment of the specification provides a culture device and a culture system. The culture device comprises a culture accommodating cavity and a culture solution accommodating cavity. The culture accommodating cavity is arranged inside the culture solution accommodating cavity. A culture solution flowing channel is formed between the outer wall of the culture accommodating cavity and the inner wall of the culture solution accommodating cavity. The culture accommodating chamber comprises a chamber bottom wall and a chamber side wall enclosing the periphery of the chamber bottom wall. The side wall of the chamber is provided with a film layer at two sides of the culture accommodating cavity along the width direction. The culture liquid flow path flows at one end portion in the longitudinal direction of the culture accommodating chamber, passes through the outside of the film layer on both sides of the culture accommodating chamber, and merges at the other end portion in the longitudinal direction of the culture accommodating chamber. The present specification also provides a culture system comprising at least two culture devices as described above.

Description

Culture device and culture system

Technical Field

The present disclosure relates to the field of medical devices, and more particularly, to a culture apparatus and a culture system.

Background

The existing cell culture chamber products, such as culture dishes, culture plates, culture bottles and the like, are inoculated with cells on the culture surfaces of the materials, and the culture space can only be the space where a two-dimensional plane is located, and cannot be expanded into a three-dimensional space, so that the utilization rate of the culture space is low, and the cell culture chamber products are not suitable for large-batch 3D cell culture (including suspension culture of single dispersed cells and 3D culture supported by a three-dimensional bracket). When the culture solution is updated, on one hand, the cells can be scratched and other external force damages are caused, and on the other hand, the nutrient generated by the cells cannot be trapped by the product.

In the case of cell culture using a 3D culture apparatus, a membrane layer may be provided in a culture chamber of the culture apparatus, and a culture solution may enter and exit the culture chamber through the membrane layer due to selective filtration of the membrane layer, so as to perform stationary culture or perfusion culture on a culture. However, different arrangements of the culture fluid flow channels (for example, the culture fluid flow channels arranged on a single side of the culture chamber) may affect that the culture fluid cannot uniformly permeate into the culture chamber, so that nutrients in the culture chamber are not uniform, and the problem of different cell culture schedules occurs.

SUMMERY OF THE UTILITY MODEL

One of the embodiments of the present specification provides a culture apparatus, characterized in that: comprises a culture accommodating cavity and a culture solution accommodating cavity; the culture accommodating cavity is arranged inside the culture solution accommodating cavity; a culture solution flowing channel is formed between the outer wall of the culture accommodating cavity and the inner wall of the culture solution accommodating cavity; the culture accommodating cavity comprises a cavity bottom wall and a cavity side wall enclosing the periphery of the cavity bottom wall; the side wall of the chamber is provided with film layers at two sides of the culture accommodating cavity along the width direction; wherein the culture solution flow path has a closed structure of "split-flow and confluence", and flows through from one end in the longitudinal direction of the culture accommodating chamber, the outer sides of the membrane layers on both sides of the culture accommodating chamber to the other end in the longitudinal direction of the culture accommodating chamber.

In some embodiments, the culture containment chamber and the culture medium containment chamber are both symmetric about a first axis of symmetry extending along the length of the culture containment chamber.

In some embodiments, the culture solution accommodating cavity is provided with a culture solution perfusion inlet and a culture solution perfusion outlet; the culture solution perfusion inlet and the culture solution perfusion outlet are respectively arranged at two ends of the culture solution accommodating cavity along the length direction and are both positioned on the first symmetrical shaft.

In some embodiments, at least one end of the chamber sidewall of the culture-accommodating chamber in the length direction is provided with a flow-guiding edge; the structure of the diversion edge angles is symmetrical along the length direction of the culture accommodating cavity; the angle point of the flow guide edge angle extends outwards along the length direction of the culture accommodating cavity.

In some embodiments, a culture access port is provided on a top wall of the culture-accommodating chamber; a sealing cover used for sealing the culture accommodating cavity is arranged on the culture inlet and the culture outlet; the sealing cover is provided with at least one sampling port.

In some embodiments, the inner side wall of the culture access opening is in a hole shape with a large upper end and a small lower end; the lower end part of the sealing cover is provided with an outer bevel edge attached to the inner side wall of the culture access opening.

In some embodiments, the shape of the top wall of the culture containment chamber corresponds to the shape of the upper end cavity opening of the culture medium containment chamber.

In some embodiments, the chamber side wall is provided with mounting holes on both sides in the width direction; the mounting hole is internally provided with a plug-in unit; the film layer is fixedly arranged on the plug-in pulling component.

One of the embodiments of the present disclosure provides a culture system including at least two culture devices as described above.

One of the embodiments of the present specification provides a culture system, wherein the at least two culture devices are arranged in parallel; the culture solution perfusion outlets and the culture solution perfusion inlets of the adjacent culture devices are connected end to end.

Drawings

The present description will be further explained by way of exemplary embodiments, which will be described in detail by way of the accompanying drawings. These embodiments are not intended to be limiting, and in these embodiments like numerals are used to indicate like structures, wherein:

FIG. 1 is a schematic illustration of an explosive structure of a culture device according to some embodiments of the present disclosure;

FIG. 2 is a schematic diagram of a culture containment chamber according to some embodiments of the present description;

FIG. 3 is a schematic liquid flow diagram of a culture liquid flow path of a culture device according to some embodiments of the present disclosure;

FIG. 4 is a schematic longitudinal cross-sectional view in a length direction of a culture device according to some embodiments of the present description;

FIG. 5 is an enlarged schematic view at A in FIG. 4;

FIG. 6 is a schematic diagram of a culture system according to some embodiments of the present disclosure;

FIG. 7 is a schematic structural view of a culture medium accommodating chamber assembly of the culture system according to some embodiments of the present specification;

in the figure: 100. a culture device; 110. a culture-accommodating chamber; 111. a chamber bottom wall; 112. a chamber sidewall; 113. flow guide edges and corners; 114. a chamber top wall; 115. a culture inlet and outlet; 120. a culture solution accommodating chamber; 121. a culture solution perfusion inlet; 122. a culture fluid perfusion outlet; 130. a sealing cover; 131. a sampling port; 132. an outer beveled edge; 133. and (7) connecting rings.

Detailed Description

In order to more clearly illustrate the technical solutions of the embodiments of the present specification, the drawings used in the description of the embodiments will be briefly described below. It is obvious that the drawings in the following description are only examples or embodiments of the present description, and that for a person skilled in the art, the present description can also be applied to other similar scenarios on the basis of these drawings without inventive effort. Unless otherwise apparent from the context, or otherwise indicated, like reference numbers in the figures refer to the same structure or operation.

It should be understood that "mounted," "connected," and "coupled" are intended to be inclusive and mean, for example, that there may be a fixed connection, a removable connection, or an integral connection; can be mechanically or electrically connected; either directly or indirectly through intervening media, or through the communication between two elements. However, other words may be substituted by other expressions if they accomplish the same purpose.

As used in this specification and the appended claims, the terms "a," "an," "the," and/or "the" are not intended to be inclusive in the singular, but rather are intended to be inclusive in the plural, unless the context clearly dictates otherwise. In general, the terms "comprises" and "comprising" are intended to cover only the explicitly identified steps or elements as not constituting an exclusive list and that the method or apparatus may comprise further steps or elements.

In some embodiments, culture refers to a method of causing a culture (e.g., cultured cells) to survive, grow, propagate, and maintain a primary structure and function in a culture chamber that mimics a particular environment (e.g., an environment within a human or animal body, a sterile environment, an environment with a particular temperature, an environment with a particular ph, an environment that meets certain nutritional conditions, etc.). In some embodiments, a culture (e.g., a cultured sample) can be placed in the culture chamber, and nutrient-supplying culture solution can be added to the culture chamber, and the culture can propagate and grow by absorbing the nutrients in the culture solution.

A culture is understood to be an object to be cultivated. In some embodiments, the culture may comprise a sample of cells for cell culture, which may also be referred to as cultured cells.

The culture solution is a substance for providing nutrients required by the growth and the propagation of the culture, and can be prepared by combining different nutrient substances. In the process of culture, the culture solution needs to be loaded or renewed. The efficiency of the culture solution loading or renewal directly affects the culture efficiency. When cell culture is performed using a 3D culture apparatus, a membrane layer may be provided on a culture chamber of the culture apparatus, and a culture solution may enter and exit the culture chamber through the membrane layer by using selective filterability of the membrane layer, so as to perform stationary culture or perfusion culture on a culture. However, different arrangements of the culture fluid flow channels (for example, the culture fluid flow channels arranged on a single side of the culture chamber) may affect that the culture fluid cannot uniformly permeate into the culture chamber, so that nutrients in the culture chamber are not uniform, and the problem of different cell culture schedules occurs. Therefore, how to effectively and uniformly introduce the culture solution into the culture chamber and how to efficiently renew the culture solution without affecting the culture chamber is a problem to be solved.

For the above reasons, some embodiments of the present application provide a culture device including a culture-accommodating chamber and a culture-solution-accommodating chamber. The culture-accommodating chamber is used for accommodating a culture. The culture holds the chamber and sets up in the inside in culture solution holding chamber, forms culture solution flow channel between the outer wall in culture holding chamber and the inner wall in culture solution holding chamber. The chamber lateral wall of culture accommodation chamber is the rete along the width direction both sides in culture accommodation chamber. The culture solution flow channel is symmetrically arranged on the outer sides of the membrane layers on the two sides, so that nutrients can more uniformly enter the culture accommodating cavity from the membrane layers on the two sides when the culture solution is perfused from the culture solution flow channel.

The culture apparatus according to the embodiment of the present specification will be described in detail below with reference to FIGS. 1 to 5. It should be noted that the following examples are only for explaining the present application and do not constitute a limitation to the present application.

FIG. 1 is a schematic diagram of an exploded configuration of a culture device according to some embodiments of the present disclosure; FIG. 2 is a schematic diagram of a culture containment chamber according to some embodiments of the present description; FIG. 3 is a schematic liquid flow diagram of a culture liquid flow path of a culture device according to some embodiments of the present disclosure; FIG. 4 is a schematic longitudinal cross-sectional view of a culture device shown in some embodiments according to the description, taken in the length direction.

In some embodiments, a culture device 100, as shown in FIG. 1, includes a culture accommodating chamber 110 and a culture solution accommodating chamber 120, and the culture accommodating chamber 110 is disposed inside the culture solution accommodating chamber 120. In some embodiments, as shown in FIG. 1, the culture accommodating chamber 110 is placed inside the culture solution accommodating chamber 120 from top to bottom. As shown in fig. 2, the culture accommodating chamber 110 includes a chamber bottom wall 111 and a chamber side wall 112 which encloses the circumferential side of the chamber bottom wall 111; the chamber side wall 112 is provided with film layers on both sides of the culture accommodating chamber 110 in the width direction.

The rete can be the microporous membrane, and the setting of rete has made inside and outside partition with culture holding chamber 110 and culture solution holding chamber 120, can not cause the interference (such as fish tail, pollution etc.) to the cultured cell like this when changing the culture solution. Since the surface of the membrane layer is provided with the micropores, nutrients can permeate from the culture solution containing cavity 120 into the culture containing cavity 110 without affecting the nutrient exchange. In some embodiments, beneficial components such as growth factors and proteins secreted by cells can be trapped in the cell culture cavity by setting the aperture of the membrane layer, and small molecule metabolic wastes and the like are exchanged into the culture solution accommodating cavity and are carried away by culture solution renewal.

The pore diameter of the membrane layer can be different ranges, and the membrane with the pore diameter of 0.1 nm-1 nm can be used for intercepting small molecules, such as sodium ions, glucose, lactic acid, ammonia ions and the like, and is beneficial to the exchange of small molecule nutrient substances and metabolic wastes of a system. The membrane with the aperture of 1 nm-100 nm can be used for intercepting macromolecules such as protein, polysaccharide, DNA and the like, and is used for intercepting and collecting metabolites of a system. 5 nm-1 mu m can trap some particulate matters, latex, micelle and the like, and is used for delivering drugs or factors. The particles with the size of 100nm to 10 mu m can intercept some biological tissues, such as viruses, bacteria, mycoplasma, cells, extracellular exosomes and the like, and are used for intercepting the biological tissues. The biological tissue aggregate with the size of 10-1 cm can be intercepted, such as organoids, cell clusters, embryos, isolated tissues and organs and the like, and is used for intercepting the biological aggregate.

In some embodiments, the three-dimensional space defined by the chamber walls 112 and the film layers on both sides of the interior of the culture-receiving chamber 110 can receive cells for culture, and is suitable for suspension cell culture or 3D culture supported by three-dimensional scaffold materials. The higher the height of the chamber sidewall 112, the more cultured cells can be contained in the three-dimensional space inside. In some embodiments, the cell culture section is vertically or obliquely placed in the culture containing cavity 110 from top to bottom, and both sides of the cell culture section are not in contact with the membrane layer, so that cells on the cell culture section can be cultured. In some embodiments, the culture-accommodating chamber 110 has a long length and a short width, and confines the cultured cells in a narrow longitudinal three-dimensional space, and the cultured cells at different height positions in the longitudinal direction of the membrane layer have the same absorption efficiency of the culture solution to both sides of the membrane layer, and theoretically can be cultured infinitely in the longitudinal space without affecting the absorption efficiency of the culture solution.

A culture fluid flow channel is formed between the outer wall of the culture accommodating chamber 110 and the inner wall of the culture solution accommodating chamber 120, and culture solution can be perfused into the culture solution flow channel. The culture liquid can be exchanged between the culture liquid channel and the inside of the culture accommodating chamber 110 through the film layer provided on the chamber side wall 112 of the culture accommodating chamber 110.

In some embodiments, the culture liquid flow path flows from one end portion in the longitudinal direction of the culture accommodating chamber 110 (in the direction of the arrow A in FIG. 3), passes outside the film layers on both sides of the culture accommodating chamber 110, and merges at the other end portion in the longitudinal direction of the culture accommodating chamber 110. For example, the direction of the flow of the culture solution is indicated by the arrow in FIG. 3. In the perfusion culture mode, it is necessary to continuously pour a new culture solution into the culture solution flow path and take out a part of the culture solution.

In some embodiments, the culture accommodating chamber 110 and the culture solution accommodating chamber 120 are symmetrical about a first axis of symmetry extending along the length direction of the culture accommodating chamber 110. For example, as shown in FIG. 3, the culture-accommodating chamber 110 and the culture-liquid accommodating chamber 120 are vertically symmetrical with respect to at least a first axis of symmetry (e.g., axis of symmetry B). In some embodiments, the culture accommodating chamber 110 and the culture liquid accommodating chamber 120 are symmetrical about a second axis of symmetry extending in the width direction of the culture accommodating chamber 110. For example, as shown in FIG. 3, the culture-accommodating chamber 110 and the culture-liquid-accommodating chamber 120 may be in a bilaterally symmetric structure about a second axis of symmetry (e.g., axis of symmetry C).

In some embodiments, the present specification does not specifically limit the shape, size, and the like of the culture solution accommodating chamber 120. The culture liquid accommodating chamber 120 may be shaped and sized so that the culture accommodating chamber 110 can be accommodated therein and a culture liquid channel can be formed with the culture accommodating chamber 110. In some embodiments, the inner cross-sectional profile shape above the culture liquid accommodating chamber 120 (for placing the part of the culture accommodating chamber 110) may include a hexagon, a rectangle, or the like. For example, in the embodiment shown in FIGS. 1 and 3, the inside contour shape above the culture liquid accommodating chamber 120 is a hexagon.

In some embodiments, the culture medium accommodating chamber 120 is provided with a culture medium perfusion inlet 121 and a culture medium perfusion outlet 122. The culture solution perfusion inlet 121 and the culture solution perfusion outlet 122 are respectively provided at both ends of the culture solution accommodating chamber 120 in the longitudinal direction.

In some embodiments, as shown in FIGS. 3 and 4, the culture solution-accommodating chamber 120 has an inverted T-shaped longitudinal section. In this embodiment, the culture liquid accommodating chamber 120 can be divided into a rectangular accommodating chamber at the lower side and a hexagonal accommodating chamber at the upper side. The culture solution perfusion inlet 121 and the culture solution perfusion outlet 122 can be arranged on the rectangular accommodating cavity and are positioned on two sides of the hexagonal accommodating cavity. As shown in fig. 4, the culture solution perfusion inlet 121 and the culture solution perfusion outlet 122 are vertically disposed on the rectangular housing chamber and communicate with the culture solution flow path inside the culture solution housing chamber 120. The openings of the culture solution perfusion inlet 121 and the culture solution perfusion outlet 122 are both upward and are used for connecting an external culture solution perfusion device.

In some embodiments, the axes of the culture perfusion inlet 121 and the perfusion outlet 122 may also be disposed in the longitudinal direction of the culture medium accommodating chamber 120, and the openings of the culture perfusion inlet 121 and the perfusion outlet 122 are respectively directed to the front and back directions of the longitudinal direction of the culture medium accommodating chamber 120 (the drawing of the embodiment is not shown).

In some embodiments, as shown in fig. 2 and 3, a flow guiding corner 113 is disposed on a side wall of the chamber at an end of the culture accommodating chamber 110 facing the perfusion inlet 121 of the culture solution. The guide rib 113 may be symmetrical in the longitudinal direction of the culture accommodating chamber 110, and a sharp corner of the guide rib 113 faces the axis of the perfusion inlet 121. The culture solution flows into the culture solution accommodating cavity 120 from the culture solution perfusion inlet 121, and is evenly distributed to two sides of the cavity side wall 112 provided with the film layer after meeting the flow guide edge 113 of the culture accommodating cavity 110. The arrangement of the diversion edges 113 ensures that the culture solution is evenly shunted outside the two layers of films when flowing in the culture solution flowing channel, thereby ensuring that nutrients in the culture solution can evenly enter the culture accommodating cavity 110.

In some embodiments, the flow guiding edges 113 may be disposed at both the front and rear ends of the culture accommodating chamber 110 in the length direction, the flow guiding edges 113 at both sides may be symmetrical along the thickness direction of the culture accommodating chamber 110, the culture solution merges at the flow guiding edges 113 near the perfusion outlet 122 of the culture solution, and the flow guiding edges 113 at this position are used to ensure that the culture solution outside the two membrane layers merges at the same flow rate when merging, further ensuring that the nutrients in the culture solution can uniformly enter the culture accommodating chamber 110.

In some embodiments, as shown in FIG. 4, a culture access port 115 is provided on the chamber top wall 114 of the culture-accommodating chamber 110. The culture port 115 is used for seeding and taking out cultured cells. The culture inlet/outlet 115 may be elongated in the longitudinal direction of the culture accommodating chamber 110, and the horizontal cross-sectional area of the culture inlet/outlet 115 is slightly smaller than the horizontal cross-sectional area of the inner cavity of the culture accommodating chamber 110. As large a culture access port 115 as possible is provided for the convenience of seeding and taking out cultured cells during the cell culture process.

A seal cover 130 for sealing the culture accommodating chamber 110 is connected to the culture inlet/outlet 115. The sealing cap 130 is detachably coupled to the culture access port 115.

The sealing cap 130 is provided with at least one sampling port 131. In some embodiments, as shown in FIG. 4, two sampling ports 131 may be provided on the sealing cap 130. In some embodiments, the sampling port 131 may be connected to an external automatic sampling device to achieve automatic sampling.

In some embodiments, as shown in FIG. 5, the inner sidewall of the culture access opening 115 has a hole shape with a large upper end and a small lower end. The lower end of the coupled sealing cap 130 is provided with an outer inclined edge 132 to be adhered to the inner sidewall of the culture gateway 115. In some embodiments, the sealing cover 130 may be made of a material with elasticity, and when the sealing cover 130 is used for sealing the upper opening of the culture access opening 115, the outer inclined edge 132 of the lower end of the sealing cover 130 forms an interference fit with the inner sidewall of the culture access opening 115, so as to ensure the sealing property.

In some embodiments, the sealing cap 130 may further be provided with a connection ring 133 at the outside of the lower end thereof. A catching groove for receiving the upper end of the culture inlet/outlet 115 is formed between the outer inclined edge 132 of the lower end of the sealing cap 130 and the coupling ring 133. When the sealing cap 130 is connected to the culture inlet/outlet 115, the upper end of the culture inlet/outlet 115 is inserted into the locking groove at the lower end of the sealing cap 130, and the outer inclined edge 132 at the lower end of the sealing cap 130 forms an interference fit with the inner side wall of the culture inlet/outlet 115, thereby ensuring sealing performance and connection firmness.

In some embodiments, as shown in FIGS. 1 and 2, the shape of the chamber top wall 114 of the culture accommodating chamber 110 corresponds to the shape of the upper end chamber opening of the culture solution accommodating chamber 120. When the culture accommodating chamber 110 is placed in the culture solution accommodating chamber 120, the chamber top wall 114 of the culture accommodating chamber 110 can be used for covering the upper end chamber opening of the culture solution accommodating chamber 120, so that the culture solution accommodating chamber 120 forms a relatively closed space.

In some embodiments, a sealing strip may be disposed on a portion of the lower end of the top wall 114 of the chamber, which contacts with the upper end surface of the culture solution containing cavity 120, and a corresponding sealing strip slot is formed on the upper end surface of the culture solution containing cavity 120. When the culture accommodating cavity 110 is placed in the culture solution accommodating cavity 120, the sealing strip is embedded into the clamping groove of the sealing strip to enhance the sealing effect.

In some embodiments, the chamber sidewall 112 is provided with mounting holes (not shown) at both sides in the width direction. The mounting hole is internally provided with a plug-in pulling component. The film layer is fixedly arranged on the plug-in unit. The plug-in pulling piece can be connected in the mounting hole in the modes of clamping, sticking, welding and the like. Because different film layers are required to be used in different culture experiments, in the embodiment, the film layers are firstly fixedly installed on the plug-in components, so that the plug-in components can be properly selected to be installed on the side wall 112 of the chamber according to different experiments, and the culture device has stronger applicability.

The present specification also provides a culture system comprising at least two culture devices as described in any of the above embodiments. As shown in FIG. 6, the culture system 200 includes five culture devices 100. In some embodiments, the culture system 200 also includes two, three, four, six, or even more culture devices 100, and the number of culture devices 100 can be selected according to experimental needs.

In some embodiments, at least two culture devices 100 of culture system 200 are arranged side-by-side. As shown in FIG. 6, five culture apparatuses 100 are arranged side by side in the width direction to constitute a culture system 200.

In some embodiments, at least two culture medium accommodating chambers 120 of at least two culture devices 100 may be fixedly coupled together. For example, as shown in FIG. 7, five culture solution accommodating chambers 120 are arranged side by side in the width direction and fixed together to form a whole culture solution accommodating chamber base. In some embodiments, the base of the culture solution containing cavity shown in fig. 7 can be formed by fixedly connecting a plurality of culture solution containing cavities 120 by clamping, adhering, riveting or integral injection molding.

In some embodiments, the perfusion outlet and perfusion inlet of adjacent culture devices of the culture system 200 are connected end-to-end. As shown in FIG. 6, the culture system 200 includes five culture devices 100, and accordingly, there are five perfusion inlets A1, A2, A3, A4, A5 for culture solution and five perfusion outlets B1, B2, B3, B4, B5 for culture solution. The culture solution perfusion outlet B1 and the culture solution perfusion inlet A2 can be connected through a pipeline, the culture solution perfusion outlet B2 and the culture solution perfusion inlet A3 can be connected through a pipeline, the culture solution perfusion outlet B3 and the culture solution perfusion inlet A4 can be connected through a pipeline, and the culture solution perfusion outlet B4 and the culture solution perfusion inlet A5 can be connected through a pipeline. Therefore, the culture medium flows into the culture medium channel in the first culture medium accommodating chamber 120 from the culture medium perfusion inlet A1, flows into the culture medium channel in the second culture medium accommodating chamber 120 from the culture medium perfusion outlet B1 to the culture medium perfusion inlet A2, sequentially passes through the culture medium channels in the third, fourth and fifth culture medium accommodating chambers 120, and finally flows out from the culture medium perfusion outlet B5.

The cell culture can be performed by the culture system 200 including a plurality of culture apparatuses 100, and the culture efficiency can be improved. For example, different kinds of cells using the same culture solution can be simultaneously cultured in different culture apparatuses 100 of the culture system 200.

The beneficial effects that may be brought by the embodiments of the present specification include, but are not limited to: 1) The culture device of the specification is convenient for perfusion culture, can improve culture space and improve cell activity; 2) The cultured cells and the flowing culture solution are separated by the membrane layer, so that the damage of the shearing force brought by the flowing culture solution to the cultured cells can be reduced, and the effect of microscope observation below the culture device cannot be influenced by the arrangement of the membrane layer; 3) The culture accommodating cavity is arranged in the culture solution accommodating cavity to form culture solution flowing channels which are symmetrically distributed on the outer sides of the two film layers, and culture solution flowing in the same flow direction is arranged on the two sides of the film layers, so that the culture solution can be fully exchanged between the inner side and the outer side of the culture accommodating cavity; 4) A larger culture access opening is formed in the culture accommodating cavity, so that operations such as inoculation, collection and the like of cultured cells are facilitated; 5) Set up a sealed lid that is used for inclosed detachable connection at the culture access & exit, be convenient for when opening cultivate cell inoculation and operation such as collection, cover the time and seal culture holding chamber, ensure the cultivation progress of inside culture cell.

Having thus described the basic concept, it will be apparent to those skilled in the art that the foregoing detailed disclosure is to be regarded as illustrative only and not as limiting the present specification. Various modifications, improvements and adaptations to the present description may occur to those skilled in the art, although not explicitly described herein. Such alterations, modifications, and improvements are intended to be suggested in this specification, and are intended to be within the spirit and scope of the exemplary embodiments of this specification.

Also, the description uses specific words to describe embodiments of the description. Reference throughout this specification to "one embodiment," "an embodiment," and/or "some embodiments" means that a particular feature, structure, or characteristic described in connection with at least one embodiment of the specification is included. Therefore, it is emphasized and should be appreciated that two or more references to "an embodiment" or "one embodiment" or "an alternative embodiment" in various places throughout this specification are not necessarily all referring to the same embodiment. Furthermore, certain features, structures, or characteristics may be combined as suitable in one or more embodiments of the specification.

Similarly, it should be noted that in the preceding description of embodiments of the present specification, various features are sometimes grouped together in a single embodiment, figure, or description thereof for the purpose of streamlining the disclosure aiding in the understanding of one or more of the embodiments. This method of disclosure, however, is not intended to imply that more features than are expressly recited in a claim. Indeed, the embodiments may be characterized as having less than all of the features of a single embodiment disclosed above.

Finally, it should be understood that the embodiments described herein are merely illustrative of the principles of the embodiments of the present disclosure. Other variations are also possible within the scope of this description. Thus, by way of example, and not limitation, alternative configurations of the embodiments of the present specification can be seen as consistent with the teachings of the present specification. Accordingly, the embodiments of the present description are not limited to only those embodiments explicitly described and depicted herein.

Claims (10)

1. A culture device, characterized by: comprises a culture accommodating cavity and a culture solution accommodating cavity; the culture accommodating cavity is arranged inside the culture solution accommodating cavity; a culture solution flowing channel is formed between the outer wall of the culture accommodating cavity and the inner wall of the culture solution accommodating cavity;

the culture accommodating cavity comprises a cavity bottom wall and a cavity side wall enclosing the periphery of the cavity bottom wall; the side wall of the chamber is provided with film layers at two sides of the culture accommodating cavity along the width direction;

wherein the culture liquid flow path has a closed structure of "split-confluence" and flows through an end portion of one end in the longitudinal direction of the culture accommodating chamber, the outer sides of the membrane layers on both sides of the culture accommodating chamber, to an end portion of the other end in the longitudinal direction of the culture accommodating chamber.

2. The culture device of claim 1, wherein: the culture accommodating cavity and the culture solution accommodating cavity are symmetrical about a first symmetry axis, and the first symmetry axis extends along the length direction of the culture accommodating cavity.

3. The culture device of claim 2, wherein: the culture solution accommodating cavity is provided with a culture solution perfusion inlet and a culture solution perfusion outlet; the culture solution perfusion inlet and the culture solution perfusion outlet are respectively arranged at two ends of the culture solution accommodating cavity along the length direction and are both positioned on the first symmetry axis.

4. The culture device of claim 1, wherein: at least one end part of the side wall of the chamber of the culture accommodating cavity along the length direction is provided with a flow guide edge angle; the structure of the diversion edge angles is symmetrical along the length direction of the culture accommodating cavity; the angle point of the flow guide edge angle extends outwards along the length direction of the culture accommodating cavity.

5. The culture device of claim 1, wherein: the top wall of the culture accommodating cavity is provided with a culture inlet and a culture outlet; a sealing cover used for sealing the culture accommodating cavity is arranged on the culture inlet and the culture outlet; the sealing cover is provided with at least one sampling port.

6. The culture device of claim 5, wherein: the inner side wall of the culture inlet and outlet is in a hole shape with a large upper end and a small lower end; the lower end part of the sealing cover is provided with an outer bevel edge attached to the inner side wall of the culture access opening.

7. The culture device of claim 1, wherein: the shape of the top wall of the culture accommodating cavity corresponds to the shape of the opening of the upper end cavity of the culture solution accommodating cavity.

8. The culture device of claim 1, wherein: mounting holes are formed in the two sides of the side wall of the chamber in the width direction; the mounting hole is internally provided with a plug-in unit; the film layer is fixedly arranged on the plug-in pulling component.

9. A culture system, characterized by: comprising at least two culture devices according to any one of claims 1 to 8.

10. The culture system of claim 9, wherein: the at least two culture devices are arranged in parallel; the culture solution perfusion outlets and the culture solution perfusion inlets of the adjacent culture devices are connected end to end.

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