CN217946037U - Cell fusion device suitable for monoclonal antibody preparation - Google Patents
Cell fusion device suitable for monoclonal antibody preparation Download PDFInfo
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- CN217946037U CN217946037U CN202221576141.4U CN202221576141U CN217946037U CN 217946037 U CN217946037 U CN 217946037U CN 202221576141 U CN202221576141 U CN 202221576141U CN 217946037 U CN217946037 U CN 217946037U
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Abstract
The application provides a cell fusion device suitable for monoclonal antibody preparation, and belongs to the field of cell fusion devices. The cell fusion device suitable for preparing the monoclonal antibody comprises a box body and a buffer mechanism, wherein a box cover is hinged to the top of the box body, a handle is arranged at the top of the box cover, a sliding groove is formed in the top of a mounting seat, a sliding block is connected with the inner side wall of the sliding groove through a first spring, a movable plate is arranged inside the box body in a sliding mode, rotating rods are hinged to two sides of the bottom of the movable plate, the two rotating rods are respectively connected with the adjacent sliding blocks in a rotating mode, vertical plates are fixed to two sides of the top of the movable plate, ejector rods penetrate through the vertical plates in a threaded mode, a clamping plate is connected to one end, opposite to the ejector rods, of the cell fusion instrument body, the cell fusion instrument body is arranged at the top of the movable plate, and the cell fusion instrument is convenient to protect.
Description
Technical Field
The application relates to the field of cell fusion devices, in particular to a cell fusion device suitable for monoclonal antibody preparation.
Background
The monoclonal antibody technology is an immunological technology, and is characterized by that the single B lymphocyte producing antibody and bone marrow tumor cell are cell-fused so as to obtain hybrid cell capable of producing antibody and limitless proliferation, and the antibody can be produced by using said hybrid cell. The cell fusion technology has been widely applied in the fields of agriculture, medicine, environmental protection and the like, and the main methods for fusion at present mainly comprise a Sendai virus induction method, an electrofusion method and a polyethylene glycol (PEG) induction method. The existing cell fusion device suitable for preparing monoclonal antibody usually comprises a cell fusion instrument, and the cell fusion instrument is usually adopted for operation when the device is used for cell fusion.
However, in the transportation process of the cell fusion device, the cell fusion instrument inside the cell fusion device is easy to shake, so that the cell fusion instrument is easy to collide with other objects inside the box body, and electric elements inside the cell fusion instrument are damaged due to collision, so that the use of the device is influenced.
SUMMERY OF THE UTILITY MODEL
In order to remedy the above deficiencies, the present application provides a cell fusion device suitable for monoclonal antibody production, aiming at improving the above-mentioned problems.
The embodiment of the application provides a cell fusion device suitable for monoclonal antibody preparation, including box and buffer gear, the box top articulates there is the case lid, the case lid top is provided with the handle, case lid bottom one side is provided with the mounting, the mounting with box lateral part swing joint, buffer gear includes mount pad, fly leaf and cell fusion appearance body, the mount pad set up in the box bottom, the spout has been seted up at the mount pad top, it is provided with the slider to slide in the spout, the slider is provided with two, be provided with first spring in the spout, two the slider passes through first spring links to each other, the slider with pass through equally between the spout inside wall first spring links to each other, the fly leaf slide set up in inside the box, fly leaf bottom both sides all articulate has the bull stick, two the bull stick respectively with adjacent nearly the slider rotates to be connected, fly leaf top both sides all are fixed with the riser, two equal screw thread has run through on the riser has the ejector pin, two the relative one end of ejector pin all rotates and is connected with splint, cell fusion appearance body set up in the fly leaf top.
In the above-mentioned implementation process, the cell fusion appearance body is for being used for carrying out the equipment that the cell fuses, and the cell fusion appearance is current equipment, specific theory of operation is not repeated herein much, open the case lid, put the cell fusion appearance body into the fly leaf, the ejector pin of rotating both sides, make both sides ejector pin be close to mutually, and then the splint that drive both sides are close to each other, thereby carry out the centre gripping to the cell fusion appearance body and fix, later cover the case lid, because case lid bottom one side is provided with the mounting, mounting and box lateral part swing joint, so in order to accomplish the process of being connected between case lid and the box through the mounting, when pulling the handle removal, can drive the cell fusion appearance body of box inside and remove, because the fly leaf slides and sets up inside the box, fly leaf bottom both sides all articulate there is the bull stick, two bull sticks respectively with the slider rotation that is close to mutually, because the cell fusion appearance body has been fixed at the fly leaf top, so when the cell fusion appearance body takes place to rock, will drive the fly leaf of its bottom and rock along with it, the bull sticks to take place to rotate this moment, the slider of both sides carries out the motion that the collision after the first spring carries out the flexible effort, thereby the cell fusion appearance body has the buffering of the cell to the cell fusion appearance body and to avoid the cell to take place to rock the trend of the process of the cell, thereby the cell fusion appearance body, thereby the buffering of the cell to the buffering of the cell fusion appearance body, thereby the buffering process of the cell fusion appearance body.
In a specific embodiment, the mounting includes extension board and screw rod, the extension board set up in case lid bottom one side, the screw rod screw thread run through in the extension board, box one side top is provided with the fixed block, fixed block one side is seted up and is supplied the through-hole that the screw rod was pegged graft.
In the implementation process, after the box cover covers the top of the box body, the screw rods are rotated to enter the aligned through holes, so that the connection process between the extension plate and the fixed block is completed, and the fixing process after the box cover covers can be completed simply and conveniently.
In a specific implementation scheme, the box is provided with the slide bar on one side of the diapire, the sliding sleeve is equipped with the sliding sleeve on the slide bar, fly leaf one end is provided with the connecting rod, the connecting rod is kept away from the one end of fly leaf with the sliding sleeve is fixed mutually.
At above-mentioned realization in-process, the sliding sleeve through fly leaf one end slides and cup joints in the setting of slide bar to carry on spacingly to the fly leaf, make the fly leaf only carry out vertical removal, thereby avoid cell fusion appearance body to take place to rock and cause the fly leaf to take place to incline often.
In a specific embodiment, the number of the sliding rods is two, the two sliding rods are symmetrically arranged relative to the mounting seat, and the connecting rod is arranged corresponding to the sliding rods.
In the implementation process, the two sliding rods are correspondingly provided with the two sliding sleeves, the connecting rods on the two sides are respectively connected with the sliding sleeves on the two sides, and through the design, the two ends of the movable plate are vertically limited.
In a specific embodiment, cell fusion appearance body both sides all are provided with the push down subassembly, two the push down subassembly is fixed in two respectively the riser top, the push down subassembly includes electric putter, plectane, stand, locating part and diaphragm, the electric putter output shaft with the plectane links to each other, stand bottom through the bearing with the plectane top rotates to be connected, the locating part is fixed set up in the stand outside, the locating part with plectane top swing joint, the diaphragm is fixed in the stand top, diaphragm bottom one side is provided with the second spring, the second spring bottom is provided with the clamp plate.
In the above-mentioned implementation process, after the cell fusion appearance body is placed at the fly leaf top, flexible through electric putter output shaft, so that the clamp plate is located cell fusion appearance body side top, because the locating part is fixed to be set up in the stand outside, locating part and plectane top swing joint, so rotate the stand to 180 degrees, make the clamp plate be located directly over the cell fusion appearance body, accomplish the fixed of stand position through the locating part, pass through electric putter once more this moment, so that the clamp plate moves down and contacts cell fusion appearance body top, so as to carry on spacingly to the direction of height of cell fusion appearance body, through the setting of second spring position, in order to realize the vertical removal of cell fusion appearance body within a certain range, thereby so that accomplish the buffer process of vertical direction when cell fusion appearance body rocks.
In a specific embodiment, the locating part includes lagging and bolt, the lagging fixed cup joint the stand is outside, threaded mounting has on the lagging the bolt, the plectane top has seted up and has supplied the round hole that the bolt was pegged graft, the round hole is provided with two, two the round hole about the plectane center is the symmetry setting.
In the implementation process, the bolt is vertically aligned with the round hole on one side, the upright post is rotated to 180 degrees through the sleeve plate, the bolt is vertically aligned with the round hole on the other side, and the bolt is rotated to enter the aligned round holes so as to complete the fixation of the position of the upright post after rotation.
In a specific embodiment, the second springs are provided in a plurality, and the second springs are uniformly distributed between the transverse plate and the pressing plate.
In the implementation process, the second springs are uniformly distributed, so that the pressing plate is always parallel to the transverse plate when moving.
In a specific embodiment, the two clamping plates are provided with anti-skid pads at the opposite ends.
In the implementation process, the anti-slip pad is arranged so as to increase the friction force when the splint is in contact with the cell fusion instrument.
In a specific embodiment, an anti-slip sleeve is fixedly sleeved on the handle.
In the implementation process, the anti-slip sleeve is fixedly sleeved outside the handle, and the handle is conveniently and better held by hands through the arrangement of the anti-slip sleeve.
In a specific embodiment, the front end of the box body is provided with a viewing window.
In the implementation process, the observation window is arranged so as to observe the condition of the cell fusion instrument body in the box body.
Drawings
In order to more clearly illustrate the technical solutions of the embodiments of the present application, the drawings that are required to be used in the embodiments will be briefly described below, it should be understood that the following drawings only illustrate some examples of the present application and therefore should not be considered as limiting the scope, and that those skilled in the art can also derive other related drawings based on these drawings without inventive effort.
FIG. 1 is a schematic diagram of a cell fusion apparatus suitable for preparing monoclonal antibodies according to an embodiment of the present disclosure;
FIG. 2 is a front cross-sectional view of a case provided in an embodiment of the present application;
FIG. 3 is a schematic structural diagram of a damping mechanism according to an embodiment of the present disclosure;
FIG. 4 is an enlarged view of A in FIG. 3 provided by an embodiment of the present application;
FIG. 5 is a top view of the circular plate of FIG. 4 according to an embodiment of the present disclosure;
fig. 6 is a front view of the exterior of the housing provided in an embodiment of the present application.
In the figure: 100-a box body; 110-box cover; 120-a handle; 121-anti-slip cover; 130-a fixture; 131-an extension plate; 132-a screw; 140-fixed block; 150-a slide bar; 151-a sliding sleeve; 160-observation window; 200-a buffer mechanism; 210-a mount; 211-a chute; 212-a slider; 213-a first spring; 220-a movable plate; 221-rotating rods; 222-a riser; 223-a top rod; 224-a splint; 225-connecting rod; 230-cell fusion instrument body; 240-a hold down assembly; 241-an electric push rod; 242-circular plate; 2421-round hole; 243-upright post; 244-a stop; 2441-sheathing; 2442-bolt; 245-a transverse plate; 2451-a second spring; 2452 pressing plate.
Detailed Description
The technical solutions in the embodiments of the present application will be described below with reference to the drawings in the embodiments of the present application.
To make the objects, technical solutions and advantages of the embodiments of the present application clearer, the technical solutions of the embodiments of the present application will be clearly and completely described below with reference to the drawings in the embodiments of the present application, and it is obvious that the described embodiments are some embodiments of the present application, but not all embodiments. All other embodiments obtained by a person of ordinary skill in the art without any inventive work based on the embodiments in the present application are within the scope of protection of the present application.
Referring to fig. 1-6, the present application provides a cell fusion device suitable for monoclonal antibody preparation, including a box 100 and a buffer mechanism 200, the top of the box 100 is hinged to a box cover 110, the top of the box cover 110 is provided with a handle 120, one side of the bottom of the box cover 110 is provided with a fixing member 130, the fixing member 130 is movably connected to the side of the box 100, the buffer mechanism 200 includes a mounting seat 210, a movable plate 220 and a cell fusion instrument body 230, the mounting seat 210 is disposed at the bottom of the box 100, the top of the mounting seat 210 is provided with a sliding groove 211, the sliding groove 211 is slidably provided with two sliding blocks 212, the sliding blocks 212 are disposed in the sliding groove 211, a first spring 213 is disposed in the sliding groove 211, the two sliding blocks 212 are connected to each other through the first spring 213, the sliding blocks 212 are also connected to the inner side wall of the sliding groove 211, the movable plate 220 is slidably disposed in the box 100, both sides of the bottom of the movable plate 220 are hinged to rotating rods 221, the two rotating rods 221 are respectively rotatably connected to the adjacent sliding blocks 212, both sides of the top of the movable plate 220 are fixed to vertical plates 222, two vertical plates 222 are threaded through ejector rods 223, one end of the opposite ends of the two ejector rods 223 are rotatably connected to each other, and the clamp plate 224, and the cell fusion instrument body 230 is disposed at the top of the movable plate 220.
In some embodiments, the fixing member 130 includes an extending plate 131 and a screw 132, the extending plate 131 is disposed at one side of the bottom of the case cover 110, the screw 132 is threaded through the extending plate 131, a fixing block 140 is disposed at a top of one side of the case body 100, a through hole for the screw 132 to be inserted is disposed at one side of the fixing block 140, and after the case cover 110 covers the top of the case body 100, the screw 132 is rotated to enter the aligned through hole (not shown in the through hole), thereby completing a connection process between the extending plate 131 and the fixing block 140, which can more easily complete a fixing process after the case cover 110 covers.
In some specific embodiments, a sliding rod 150 is disposed on one side of the bottom wall of the box 100, a sliding sleeve 151 is slidably sleeved on the sliding rod 150, a connecting rod 225 is disposed at one end of the movable plate 220, one end of the connecting rod 225 far away from the movable plate 220 is fixed to the sliding sleeve 151, and the sliding sleeve 151 at one end of the movable plate 220 is slidably sleeved on the sliding rod 150 to limit the movable plate 220, so that the movable plate 220 can only vertically move, thereby preventing the movable plate 220 from tilting when the cell fusion instrument body 230 rocks.
In some specific embodiments, two sliding rods 150 are provided, two sliding rods 150 are symmetrically arranged with respect to the mounting base 210, the connecting rod 225 is arranged corresponding to the sliding rods 150, two sliding sleeves 151 are correspondingly arranged on the two sliding rods 150, and the connecting rods 225 on both sides are respectively connected with the sliding sleeves 151 on both sides, so that both ends of the movable plate 220 are vertically limited.
In some specific embodiments, the cell fusion instrument body 230 is provided with pressing assemblies 240 at both sides, the two pressing assemblies 240 are respectively fixed on the tops of the two vertical plates 222, each pressing assembly 240 includes an electric push rod 241, a circular plate 242, an upright 243, a limiting member 244 and a horizontal plate 245, an output shaft of the electric push rod 241 is connected with the circular plate 242, the bottom of the upright 243 is rotatably connected with the top of the circular plate 242 through a bearing, the limiting member 244 is fixedly arranged outside the upright 243, the limiting member 244 is movably connected with the top of the circular plate 242, a second spring 2451 is arranged on one side of the bottom of the horizontal plate 245, a pressing plate 2452 is arranged at the bottom of the second spring 2451, when the cell fusion instrument body 230 is placed on the top of the movable plate 220, the pressing plate 2452 is positioned right above the cell fusion instrument body 230 through the extension and retraction of the output shaft of the electric push rod 241, the position of the upright 230 is fixed through the limiting member 244, the limiting member 244 is movably connected with the top of the upright 243, the limiting member 244 is rotated to 180 degrees, so that the upright 243 is positioned right above the cell fusion instrument body 230, and the electric push rod 241 is moved to the lower plate 230, and the cell fusion instrument body is moved to buffer the cell fusion instrument body 230, so that the cell fusion instrument body 230 is vertically moved in a cell fusion process, and the cell fusion instrument body 230, thereby achieving a cell fusion process.
In some specific embodiments, the limiting member 244 includes a sleeve plate 2441 and a bolt 2442, the sleeve plate 2441 is fixedly fastened to the outside of the upright 243, the bolt 2442 is threadedly mounted on the sleeve plate 2441, a circular hole 2421 for the bolt 2442 to be inserted into is formed at the top of the circular plate 242, two circular holes 2421 are formed in the circular holes 2421, the two circular holes 2421 are symmetrically arranged with respect to the center of the circular plate 242, the bolt 2442 is initially vertically aligned with the circular hole 2421 on one side, the upright 243 is rotated to 180 degrees through the sleeve plate 2441, so that the bolt 2442 is vertically aligned with the circular hole 2421 on the other side, and the bolt 2442 is rotated into the aligned circular holes 2421, so as to complete the fixing of the position where the upright 243 rotates.
In some specific embodiments, the number of the second springs 2451 is several, and several second springs 2451 are uniformly distributed between the cross plate 245 and the pressing plate 2452, so that the pressing plate 2452 is always parallel to the cross plate 245 when moving through the arrangement of the second springs 2451 uniformly distributed.
In some embodiments, the opposing ends of the two clamping plates 224 are each provided with a non-slip pad, which is configured to increase the friction between the clamping plates 224 and the cell fusion instrument body 230 during contact.
In some specific embodiments, the anti-slip sleeve 121 is fixedly sleeved on the handle 120, and the anti-slip sleeve 121 is fixedly sleeved outside the handle 120, so that a human hand can better hold the handle 120 through the arrangement of the anti-slip sleeve 121.
In some embodiments, the front end of the casing 100 is provided with an observation window 160, and the observation window 160 is arranged to observe the condition of the cell fusion instrument body 230 inside the casing 100.
The working principle of the cell fusion device suitable for preparing the monoclonal antibody is as follows: the cell fusion instrument body 230 is an apparatus for cell fusion, the cell fusion instrument is an existing apparatus, the specific working principle is not described herein, the box cover 110 is opened, the cell fusion instrument body 230 is placed on the movable plate 220, the ejector rods 223 at two sides are rotated to make the ejector rods 223 at two sides approach each other, and then the clamping plates 224 at two sides approach each other, so as to clamp and fix the cell fusion instrument body 230, then the box cover 110 is covered, because the fixing member 130 is arranged at one side of the bottom of the box cover 110, the fixing member 130 is movably connected with the side portion of the box body 100, so that the connection process between the box cover 110 and the box body 100 is completed through the fixing member 130, when the handle 120 is pulled to move, the cell fusion instrument body 230 inside the box body 100 can be driven to move, because the movable plate 220 is slidably arranged inside the box body 100, the rotating rods 221 are hinged at two sides of the bottom of the movable plate 220, the two rotating rods 221 are respectively rotatably connected with the adjacent sliders 212, because the cell fusion instrument body 230 is fixed at the top of the movable plate 220, when the cell fusion instrument body 230 shakes, the movable plate 220 rotates, so as to avoid the cell fusion instrument body 230 from moving by the first telescopic spring 213, and the first telescopic spring 213, thereby avoiding the cell fusion instrument body 230 from moving.
It should be noted that the specific model specifications of the cell fusion instrument body 230 and the electric push rod 241 need to be determined by type selection according to the actual specification of the device, and the specific type selection calculation method adopts the prior art, so detailed description is omitted.
The power supply and the principle of the cell fusion instrument body 230 and the electric push rod 241 are clear to those skilled in the art, and will not be described in detail herein.
The above description is only an example of the present application and is not intended to limit the scope of the present application, and various modifications and changes may be made to the present application by those skilled in the art. Any modification, equivalent replacement, improvement and the like made within the spirit and principle of the present application shall be included in the protection scope of the present application. It should be noted that: like reference numbers and letters refer to like items in the following figures, and thus, once an item is defined in one figure, it need not be further defined and explained in subsequent figures.
The above description is only for the specific embodiments of the present application, but the scope of the present application is not limited thereto, and any person skilled in the art can easily conceive of the changes or substitutions within the technical scope of the present application, and shall be covered by the scope of the present application. Therefore, the protection scope of the present application shall be subject to the protection scope of the claims.
Claims (10)
1. A cell fusion device suitable for monoclonal antibody preparation, comprising
The box body (100), the top of the box body (100) is hinged with a box cover (110), the top of the box cover (110) is provided with a handle (120), one side of the bottom of the box cover (110) is provided with a fixing piece (130), and the fixing piece (130) is movably connected with the side part of the box body (100);
the buffer mechanism (200) comprises a mounting seat (210), a movable plate (220) and a cell fusion instrument body (230), the mounting seat (210) is arranged at the bottom in the box body (100), a sliding groove (211) is formed in the top of the mounting seat (210), sliding blocks (212) are arranged in the sliding groove (211), two sliding blocks (212) are arranged in the sliding groove (211), a first spring (213) is arranged in the sliding groove (211), the two sliding blocks (212) are connected through the first spring (213), the sliding blocks (212) are connected with the inner side wall of the sliding groove (211) through the first spring (213), the movable plate (220) is arranged in the box body (100) in a sliding mode, rotating rods (221) are hinged to two sides of the bottom of the movable plate (220), the two rotating rods (221) are respectively connected with the adjacent sliding blocks (212) in a rotating mode, vertical plates (222) are fixed to two sides of the top of the movable plate (220), push rods (223) penetrate through threads, one end, opposite to one end of each rotating rod (223), and the cell fusion instrument body (230) is arranged on the top of the movable plate (220).
2. The cell fusion device suitable for monoclonal antibody preparation according to claim 1, wherein the fixing member (130) comprises an extension plate (131) and a screw (132), the extension plate (131) is disposed on one side of the bottom of the case cover (110), the screw (132) is threaded through the extension plate (131), a fixing block (140) is disposed on the top of one side of the case body (100), and a through hole for the screw (132) to be inserted is formed on one side of the fixing block (140).
3. The cell fusion device suitable for preparing monoclonal antibodies according to claim 1, wherein a sliding rod (150) is disposed on one side of the bottom wall of the box (100), a sliding sleeve (151) is slidably disposed on the sliding rod (150), a connecting rod (225) is disposed at one end of the movable plate (220), and one end of the connecting rod (225) away from the movable plate (220) is fixed to the sliding sleeve (151).
4. The cell fusion device for preparing monoclonal antibodies according to claim 3, wherein the number of the slide bars (150) is two, the two slide bars (150) are symmetrically arranged about the mounting seat (210), and the connecting rods (225) are arranged corresponding to the slide bars (150).
5. The cell fusion device suitable for preparing a monoclonal antibody according to claim 1, wherein the cell fusion instrument body (230) is provided with pressing assemblies (240) at two sides, the two pressing assemblies (240) are respectively fixed on the tops of the two vertical plates (222), the pressing assemblies (240) comprise an electric push rod (241), a circular plate (242), an upright post (243), a limiting member (244) and a transverse plate (245), an output shaft of the electric push rod (241) is connected with the circular plate (242), the bottom of the upright post (243) is rotatably connected with the top of the circular plate (242) through a bearing, the limiting member (244) is fixedly arranged outside the upright post (243), the limiting member (244) is movably connected with the top of the circular plate (242), the transverse plate (245) is fixed on the top of the upright post (243), one side of the bottom of the transverse plate (245) is provided with a second spring (2451), and the bottom of the second spring (2451) is provided with a pressing plate (2452).
6. The cell fusion device suitable for monoclonal antibody preparation according to claim 5, wherein the limiting member (244) comprises a sleeve plate (2441) and a bolt (2442), the sleeve plate (2441) is fixedly sleeved outside the upright column (243), the bolt (2442) is installed on the sleeve plate (2441) in a threaded manner, a circular hole (2421) for the bolt (2442) to be inserted is formed in the top of the circular plate (242), two circular holes (2421) are formed in the circular hole (2421), and the two circular holes (2421) are symmetrically arranged with respect to the center of the circular plate (242).
7. The cell fusion device for preparing monoclonal antibody of claim 5, wherein the second springs (2451) are provided in a plurality, and the plurality of second springs (2451) are uniformly distributed between the transverse plate (245) and the pressing plate (2452).
8. The cell fusion device for preparing monoclonal antibodies of claim 1, wherein the two clamping plates (224) are provided with anti-slip pads at opposite ends.
9. The cell fusion device for preparing monoclonal antibodies of claim 1, wherein the handle (120) is fixedly sleeved with an anti-slip sleeve (121).
10. The cell fusion apparatus for preparing monoclonal antibodies according to claim 1, wherein the front end of the housing (100) is provided with an observation window (160).
Priority Applications (1)
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CN202221576141.4U CN217946037U (en) | 2022-06-22 | 2022-06-22 | Cell fusion device suitable for monoclonal antibody preparation |
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CN202221576141.4U CN217946037U (en) | 2022-06-22 | 2022-06-22 | Cell fusion device suitable for monoclonal antibody preparation |
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CN202221576141.4U Active CN217946037U (en) | 2022-06-22 | 2022-06-22 | Cell fusion device suitable for monoclonal antibody preparation |
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