CN217884712U

CN217884712U - Be used for sterile shake flask device of tissue culture explant

Info

Publication number: CN217884712U
Application number: CN202221731217.6U
Authority: CN
Inventors: 张彩玲; 李佩顺; 沈洪宽; 邢立伟; 韩超慧; 张乐宇; 薛锦远; 李潇驰
Original assignee: HEILONGJIANG AGRICULTURAL ECONOMY VOCATIONAL COLLEGE
Current assignee: HEILONGJIANG AGRICULTURAL ECONOMY VOCATIONAL COLLEGE
Priority date: 2022-07-07
Filing date: 2022-07-07
Publication date: 2022-11-25
Anticipated expiration: 2032-07-07

Abstract

The utility model provides a shake bottle device for tissue culture explant disinfection relates to plant tissue culture technical field, include base (1) and fix bottle fixture and actuating mechanism on base (1), bottle fixture includes basal disc (2), locking dish (3) and clamping jaw (4), basal disc (2) and locking dish (3) are the disk body of keeping flat, basal disc (2) are equipped with around the centre of a circle and are radial align to grid's track (5), be equipped with on track (5) along slider (6) of its removal, clamping jaw (4) are vertical respectively to be fixed on slider (6), locking dish (3) equipartition have spout (7) that one end corresponding with clamping jaw (4) quantity asymptotes to its centre of a circle, locking dish (3) rotationally are connected with the axle center with basal disc (2), clamping jaw (4) pass from spout (7) of locking dish (3). Has the advantages of simple structure, reasonable design, convenient use, quick clamping, low cost, even shaking, high efficiency and the like.

Description

Be used for sterile shake flask device of tissue culture explant

Technical Field

The utility model relates to a plant tissue culture technical field, concretely relates to a shake bottle device for tissue culture explant is disinfected.

Background

Plant tissue culture refers to a technique of isolating desired tissues, organs or cells, protoplasts, etc. from a plant body, and culturing under artificially controlled conditions by aseptic manipulation to obtain regenerated whole plants or produce other products having economic value. The process of tissue culture disinfection and the like often requires the operation of shaking the bottle so that the plant tissue in the bottle is fully contacted with the effective ingredients in the solution. The shake flasks have different durations according to different requirements, some are only a little, and some are as long as tens, twenty-few minutes or even longer. The process of manually shaking the bottle is time consuming and labor intensive, and the operator cannot free his hands to do other things. The existing bottle shaking device is usually designed only for a bottle with a specific shape, and the application range is narrow. Or the existing shake flask device is too complex in structure and high in use cost.

Disclosure of Invention

The utility model discloses the technical problem that will solve is: in response to the above problems, a shake flask apparatus for tissue culture is provided.

The technical solution of the utility model is that: the utility model provides a shake bottle device for tissue culture explant disinfection, includes the base and fixes bottle fixture and actuating mechanism on the base, and actuating mechanism drive bottle fixture operation, bottle fixture includes basal disc, locking dish and clamping jaw, and basal disc and locking dish are the disk body of keeping flat, and the basal disc is equipped with the track that is radial align to grid around the centre of a circle, is equipped with the slider that moves along it on the track, and the clamping jaw is vertical to be fixed on the slider respectively, and the locking dish equipartition has the spout that one end corresponding with clamping jaw quantity asymptotes to its centre of a circle, and the locking dish rotationally is connected with the same axle center with the basal disc, and the clamping jaw passes from the spout of locking dish, rotates the locking dish and can promote the clamping jaw through the spout and move to central direction simultaneously.

The technical effects of the utility model are that: has the advantages of simple structure, reasonable design, convenient use, quick clamping, low cost, uniform shaking, high efficiency and the like. The fitting with proper fixing function can be selected to correspond to the bottle bodies with different shapes, and the application range is wide. The fixed fittings can adopt the silica gel material that elasticity effect is good, makes its shape suitably corresponding in certain extent adjust to satisfy more bottlenecks of different specifications. The shaking process can be completed instead of manual work, so that the manpower resource is saved, and the operator can vacate hands to carry out other working steps.

Drawings

FIG. 1 is a schematic view of the three-dimensional structure of the cylindrical bottle holding state of the present invention;

FIG. 2 is a schematic structural view of the base plate and the clamping jaw part of the present invention;

FIG. 3 is a schematic sectional view of the locking plate of the present invention;

FIG. 4 is a schematic view of the three-dimensional structure of the bottle body clamping mechanism of the present invention in a state of clamping a conical bottle;

fig. 5 is a schematic structural view of the bottle body clamping mechanism and the driving mechanism of the present invention.

Detailed Description

As shown in fig. 1 to 5, a shake flask device for tissue culture explant sterilization comprises a base 1, and a bottle body clamping mechanism and a driving mechanism which are fixed on the base 1, wherein the driving mechanism drives the bottle body clamping mechanism to operate, the bottle body clamping mechanism comprises a base disc 2, a locking disc 3 and clamping jaws 4, the base disc 2 and the locking disc 3 are flat disc bodies, the base disc 2 is provided with tracks 5 which are radially and uniformly arranged around the center of a circle, the tracks 5 are provided with sliding blocks 6 which move along the tracks, the clamping jaws 4 are respectively and vertically fixed on the sliding blocks 6, the locking disc 3 is uniformly provided with sliding grooves 7, one ends of the sliding grooves 7 are gradually close to the center of the clamping jaws 4, the locking disc 3 is rotatably connected with the base disc 2 in a coaxial manner, the clamping jaws 4 penetrate through the sliding grooves 7 of the locking disc 3, and the rotating locking disc 3 can push the clamping jaws 4 to move towards the center direction through the sliding grooves 7, so that bottles arranged on the base disc 2 and the locking disc 3 can be clamped. The driving device drives the base plate 2 to rotate in the direction corresponding to the direction of the sliding groove 7 which is gradually close to the direction of the circle center, and the problem that the clamping jaw 4 is loosened can be avoided during rotation. The number of the holding jaws 4 is not less than 3.

The edge of the locking disc 3 is provided with a surrounding edge 8 which extends downwards and covers the edge of the base disc 2. Upon rotation, the peripheral edge 8 of the locking disc 3 engages with the edge of the base disc 2, thereby limiting rotation of the locking disc 3 about the base disc 2. The two can also be connected by a short axis.

The clamping jaw 4 is sleeved with a rubber sleeve 9. The lower end of the rubber sleeve 9 can be abutted to the locking disc 3, so that the locking disc 3 is limited.

The fixing accessory 10 for assisting the bottle body to be fixed is arranged between the clamping jaws 4, the fixing accessory 10 is composed of a ring sleeve for sleeving the bottle body and connecting parts, the number of the connecting parts is corresponding to that of the clamping jaws, one end of each connecting part of the fixing accessory 10 is fixedly connected with the ring sleeve, and the other end of each connecting part is provided with a round hole for sleeving the clamping jaws. The fixing fitting 10 is used after the clamping jaw 4 is tightly attached to the bottle body (the bottom of the conical bottle), and is sleeved on the upper portion of the bottle body and connected with the clamping jaw 4 to play a role in assisting in fixing the bottle body.

The fixing fitting 10 is made of an elastic rubber material and has good shape adaptability.

The driving mechanism comprises a rotating shaft 11 which is eccentrically fixed at the bottom of the base plate and is vertically arranged, and a driving motor 12 and a speed reducer 13 which are connected with the rotating shaft 11. A lower appropriate shaking speed can be generated by the reducer 13.

An annular cam 14 is arranged on a rotating shaft 11 at the bottom of the base plate 2, a protruding part of the cam 14 is arranged on the lower end face of the cam 14 and protrudes downwards, a roller 15 is arranged below the cam 14 and abuts against the lower end face of the cam 14, the roller 15 is rotatably fixed on the base 1, the rotating shaft 11 is fixed on the base 1 through a sliding sleeve 16 and can rotate and slide up and down along the sliding sleeve 16, and the rotating shaft 11 is connected with a driving motor 12 and a speed reducer 13 through a pair of gears 17 which are meshed and can slide up and down relatively. When the base disc 2 rotates under the driving of the driving motor 12, the shaking motion is generated under the action of the eccentric shaft, and the up-and-down displacement is generated under the action of the cam 14 and the roller 15, so that the shaking motion meeting the requirements is superposed.

Claims

1. The utility model provides a shake bottle device for tissue culture explant disinfection, include base (1) and fix bottle fixture and actuating mechanism on base (1), actuating mechanism drive bottle fixture operation, a serial communication port, bottle fixture includes base disc (2), locking dish (3) and clamping jaw (4), base disc (2) and locking dish (3) are the disk body of keeping flat, base disc (2) are equipped with and encircle the centre of a circle and are radial align to grid's track (5), be equipped with on track (5) along slider (6) of its removal, clamping jaw (4) vertical fixing respectively on slider (6), locking dish (3) equipartition have with clamping jaw (4) quantity corresponding one end to its centre of a circle approximate spout (7), locking dish (3) rotationally with base disc (2) coaxial coupling, clamping jaw (4) pass from spout (7) of locking dish (3), it can promote clamping jaw (4) through spout (7) and move to the central direction simultaneously to rotate locking dish (3).

2. A shake flask device for sterilization of tissue culture explants according to claim 1, characterized in that the edge of the locking disk (3) is provided with a downwardly protruding skirt (8) that covers the edge of the basal disk (2).

3. A shake flask device for tissue culture explant sterilization according to claim 1, wherein the jaws (4) are covered with rubber sleeves (9).

4. A shake flask device for sterilization of explants for tissue culture according to claim 1, 2 or 3, characterized in that between the clamping jaws (4) there is a fixing fitting (10) for assisting the fixation of the bottle, the fixing fitting (10) is composed of a collar for fitting over the bottle and a number of connections corresponding to the number of the clamping jaws, one end of the connection of the fixing fitting (10) is fixedly connected to the collar and the other end is provided with a round hole for fitting over the clamping jaw.

5. A shake flask device for tissue culture explant sterilization according to claim 4, wherein the fixation fitting (10) is made of an elastic rubber material.

6. A shake flask apparatus for tissue culture explant sterilization according to claim 1, wherein the drive mechanism comprises a shaft (11) eccentrically fixed to the bottom of the basal disc and a drive motor (12) and speed reducer (13) connected to the shaft (11).

7. A shake flask device for tissue culture explant sterilization according to claim 6, wherein the rotating shaft (11) at the bottom of the basal disc (2) is provided with a circular cam (14), the convex part of the cam (14) is arranged on the lower end surface and protrudes downwards, a roller (15) is arranged below the cam (14) and abuts on the lower end surface of the cam (14), the roller (15) is rotatably fixed on the base (1), the rotating shaft (11) is fixed on the base (1) through a sliding sleeve (16) and can rotate and slide up and down along the sliding sleeve (16), and the rotating shaft (11) is connected with the driving motor (12) and the speed reducer (13) through a pair of meshed gears (17) which can slide up and down relatively.