CN217868916U - 3D cell culture chip subassembly - Google Patents
3D cell culture chip subassembly Download PDFInfo
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- CN217868916U CN217868916U CN202222019177.9U CN202222019177U CN217868916U CN 217868916 U CN217868916 U CN 217868916U CN 202222019177 U CN202222019177 U CN 202222019177U CN 217868916 U CN217868916 U CN 217868916U
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Abstract
The utility model relates to a 3D cell culture chip subassembly, include: the chip comprises a chip body and a chip support, wherein the chip body comprises a core plate and a plurality of liquid storage tanks; the chip support is provided with a core plate mounting hole; the core plate is detachably connected in the core plate mounting hole. The utility model discloses a set up the cell culture groove that is used for cultivateing the cell adhesion at a face of core to at the fixed liquid reserve tank of another face of core, can constitute the 3D cell culture chip of a commonality, cultivate simultaneously cell and culture solution and arrange two faces of core in branch, be convenient for add respectively and cultivate cell and culture solution, thereby reduce cross contamination, reduce experimental operating procedure, and when rotating the core about the horizontal direction, can provide the static pressure for the liquid reserve tank, promote its inside culture solution to flow in the cell culture groove.
Description
Technical Field
The utility model relates to a cell culture technical field, in particular to 3D cell culture chip subassembly.
Background
Researchers are developing human organ micro-scale models on plastic chips and consider this technique, called organ chips, as a substitute for animal models. While this goal remains remote, the technology is gaining increasing attention as large pharmaceutical companies begin to use these in vitro systems in drug development. The support of organ chips alleges that the chips are closer to the actual model of the human body than flat thin-layer cells grown in culture dishes, and are more useful for drug development and testing than animal models. For example, a pulmonary chip is composed of a layer of cells, one side bathed in a blood-like culture medium and the other side exposed to air, and connected to a machine that stretches and compresses the cells to mimic respiratory motion. As research progresses, a variety of different organ chips have been developed, including, liver, intestine, lung, blood vessels, blood brain barrier, kidney, etc. Based on the above, more integrated multi-organ chips, even human body chips, have become one of the main development directions of organ chips.
Although researchers have designed a variety of multi-organ chips, these chips still dominate the flow channel, and thus require the injection of cell suspension into the microchannel during cell seeding. This operation adds some operational complexity to the user, which causes problems such as uneven cell seeding and cross contamination. In addition, at present, the organ chip mainly uses elastic material PDMS as a main material, the production period is long, the cost is high, meanwhile, the chip also needs complex operations such as sealing, punching and intubation, and the existing organ chip is basically developed in a targeted manner, so that the flexibility of experimental design is greatly limited by the special chip, and meanwhile, the cost of chip design and preparation is increased.
Therefore, how to design a universal 3D cell culture chip assembly is a problem that needs to be solved by those skilled in the art.
SUMMERY OF THE UTILITY MODEL
The utility model provides a 3D cell culture chip subassembly solves the current organ chip preparation with high costs, the complicated and relatively poor technical problem of commonality of operation.
The utility model provides a technical scheme as follows of above-mentioned technical problem, a 3D cell culture chip subassembly, include: a chip body and a chip support,
the chip body comprises a core plate and a plurality of liquid storage tanks, wherein a plurality of cell culture grooves for culturing cells are formed in one plate surface of the core plate; the liquid storage tanks are respectively fixed on the other plate surface of the core plate corresponding to the two ends of the cell culture tanks, and culture solution inlets and outlets communicated with the liquid storage tanks are formed in the bottoms of the cell culture tanks;
the chip support is provided with a core plate mounting hole; the core plate is detachably connected in the core plate mounting hole.
The utility model has the advantages that: through set up the cell culture groove that is used for cultivateing the cell adhesion at a face of core to at the fixed liquid storage tank of another face of core, can constitute the 3D cell culture chip of a commonality, two faces of core are arranged respectively in to cell and culture solution of cultivateing simultaneously, are convenient for add respectively and cultivate cell and culture solution, thereby reduce cross contamination, reduce experimental operating procedure, and when rotating the core, can provide static pressure for the liquid storage tank, promote its inside culture solution to flow in the cell culture groove.
On the basis of the technical scheme, the utility model discloses can also do following improvement.
The culture medium feeding device further comprises a plurality of liquid injection cylinders, wherein the liquid injection cylinders are respectively positioned in the liquid storage pools, and are fixed at the bottoms of the liquid storage pools corresponding to the peripheral sides of the culture medium inlet and the culture medium outlet.
Furthermore, a plurality of pipette tip placing notches are formed in the inner wall, close to the pool opening, of the liquid storage pools along the radial direction of the inner wall, and penetrate through the pool opening end face of the liquid storage pools.
The pipette head placing notch is designed, so that the pipette head of the pipette can be placed at the pipette head placing notch, and the placing stability of the pipette is improved.
Further, the height of the plurality of liquid storage tanks is as follows: 5mm-15mm, the inner hole diameter is: 6mm-10mm; the heights of the liquid injection cylinders are as follows: 3mm-5mm, and the inner diameter of the liquid injection cylinders is 0.5-1.5mm.
The cell culture device further comprises a communicating groove and a plurality of blocking columns, wherein the cell culture grooves are symmetrically and radially arranged and communicated with two ends of the communicating groove; the blocking columns are positioned in the communicating groove and are arranged into a plurality of strips at intervals, and the cell culture grooves with two symmetrically arranged ends are communicated by the strips.
Further, the width D2 of the communication groove is larger than the width D1 of the cell culture groove.
The cell culture tank has the further beneficial effects that the cell culture tanks are symmetrically and radially arranged and are communicated with two ends of the communicating tank, the communicating tank is internally provided with a plurality of strips at intervals, and the gap between every two blocking columns is an area for inoculating cells.
Furthermore, the cross section of each blocking column is circular, triangular, quadrilateral, pentagonal or teardrop-shaped, the height of each blocking column is 0.1-2mm, and the interval between every two adjacent blocking columns is 0.1-0.5 mm.
Further, the chip bracket comprises a chip mounting plate, a plurality of annular supporting plates and a plurality of elastic limit buckles,
the other plate surface of the core plate is provided with an installation groove; the liquid storage tanks are fixed at the bottoms of the mounting grooves; the chip mounting plate is provided with a plurality of core plate mounting holes penetrating through two plate surfaces of the chip mounting plate; the annular supporting plates are close to one plate surface of the chip mounting plate and are fixed on the inner wall of the core plate mounting hole in a surrounding mode; the elastic limit buckles are fixed in the middle of the hole walls of the core plate mounting holes at intervals; insert the core mounting hole one face edge of core with correspond the annular support board butt and the edge of its another face mounting groove notch and a plurality of the butt of elasticity limit buckle.
The core plate is inserted into the chip mounting hole, and the annular supporting plate and the elastic limiting buckle are adopted to limit the two plate surfaces of the core plate, so that the core plate can be conveniently fixed or detached.
The annular support plate is characterized by further comprising a plurality of elastic shifting sheets and a plurality of bulges, wherein the elastic shifting sheets are respectively fixed on the annular support plates in parallel; the plurality of bulges are respectively fixed on the end surfaces of the plurality of elastic shifting pieces close to the core plate.
The core plate ejection device has the further beneficial effects that the elastic shifting sheet and the bulge are pushed towards the core plate, so that the core plate can be ejected out, and the core plate is convenient to disassemble.
Further, the chip body and the chip support are made of PMMA (polymethyl methacrylate), PS (polystyrene), PC (polycarbonate), PET (polyethylene terephthalate), COC (chip on chip) or COP (coefficient of performance).
The chip body and the chip support are made of PMMA (polymethyl methacrylate), PS (polystyrene), PC (polycarbonate), PET (polyethylene terephthalate), COC (chip on chip) or COP (coefficient of performance), the core plate and the liquid storage tanks of the chip body can be integrally formed, and the chip mounting plate, the annular support plates and the elastic limit buckles of the chip support can also be integrally formed, so that the manufacturing cost of the 3D cell culture chip assembly is reduced, and the manufacturing difficulty of the 3D cell culture chip assembly is reduced.
Further, the length of each cell culture groove is 1.5cm-3.5cm, the width is 0.1mm-2mm, and the depth is 0.2mm-2mm.
Drawings
Fig. 1 is a schematic view of a three-dimensional structure of a chip body angle 1 in a 3D cell culture chip assembly according to the present invention;
FIG. 2 is an enlarged view of a portion of the structure at A in FIG. 1;
FIG. 3 is a schematic view of an angle 2 three-dimensional structure of a chip body in a 3D cell culture chip assembly according to the present invention;
FIG. 4 is a schematic diagram of the structure of a chip holder in a 3D cell culture chip assembly of the present invention.
In the drawings, the components represented by the respective reference numerals are listed below:
1. chip body, 11, core board, 111, cell culture groove, 112, mounting groove, 12, liquid storage tank, 121, pipette tip place the breach, 13, block post, 14, intercommunication groove, 2, chip support, 21, chip mounting panel, 211, core board mounting hole, 22, annular backup pad, 23, elasticity spacing knot, 24, elasticity plectrum, 25, arch.
Detailed Description
The principles and features of the present invention are described below in conjunction with the following drawings, the examples given are only intended to illustrate the present invention and are not intended to limit the scope of the present invention.
As shown in fig. 1, a 3D cell culture chip assembly, comprising: a chip body 1 and a chip support 2,
the chip body 1 comprises a core plate 11 and a plurality of liquid storage tanks 12, wherein a plurality of cell culture grooves 111 for culturing cells are formed in one plate surface of the core plate 11; the liquid storage tanks 12 are respectively fixed on the other plate surface of the core plate 11 corresponding to two ends of the cell culture tanks 111, and culture solution inlets and outlets communicated with the liquid storage tanks 12 are formed at the bottoms of the cell culture tanks 111;
the chip support 2 is provided with a core plate mounting hole 211; the core 11 is detachably attached in the core mounting hole 211.
In some embodiments, a plurality of liquid injection cylinders may be further included, and the plurality of liquid injection cylinders are respectively located inside the plurality of liquid storage pools 12 and fixed on the bottom of the liquid storage pools 12 corresponding to the outer peripheral sides of the culture solution inlets and outlets.
In some embodiments, the inner wall of the plurality of reservoirs 12 near the reservoir ports thereof may be provided with pipette tip placement notches 121 radially outward thereof, and the pipette tip placement notches 121 penetrate the port end faces of the reservoirs 12.
In some embodiments, the plurality of reservoirs 12 are cylindrical in configuration and may have a height of: 5mm-15mm, the inner hole diameter can be: 6mm-10mm; the height of the plurality of liquid injection cylinders can be as follows: 3mm-5mm, and the inner diameter of the liquid injection cylinders is 0.5-1.5mm.
In some embodiments, a communication groove 14 and a plurality of blocking pillars 13 may be further included, and the plurality of cell culture grooves 111 are symmetrically and radially arranged and communicated with two ends of the communication groove 14; the plurality of barrier pillars 13 are positioned in the communicating groove 14 and arranged at intervals in a plurality of strips, and the plurality of strips communicate the cell culture grooves 111 symmetrically arranged at both ends.
In some embodiments, the cross-section of the barrier pillars 13 may be circular, triangular, quadrilateral, pentagonal, or teardrop-shaped, the height of the barrier pillars 13 is 0.1-2mm, and the interval between two adjacent barrier pillars 13 is 0.1-0.5 mm.
In some embodiments, the chip holder 2 may include a chip mounting plate 21, a plurality of ring-shaped supporting plates 22 and a plurality of elastic retaining buckles 23,
the other plate surface of the core plate 11 is provided with a mounting groove 112; a plurality of liquid storage tanks 12 are fixed at the bottom of the mounting groove 112; the chip mounting plate 21 is provided with a plurality of core plate mounting holes 211 penetrating through two plate surfaces of the chip mounting plate; a plurality of annular supporting plates 22 are close to one plate surface of the chip mounting plate 21 and are fixed on the inner wall of the core plate mounting hole 211 in a surrounding manner; the elastic limit buckles 23 are fixed at the middle parts of the hole walls of the core plate mounting holes 211 at intervals; the edge of one plate surface of the core plate 11 inserted into the core plate mounting hole 211 is abutted with the corresponding annular support plate 22, and the edge of the notch of the mounting groove 112 of the other plate surface is abutted with the plurality of elastic limit buckles 23.
In some embodiments, a plurality of elastic pulling pieces 24 and a plurality of protrusions 25 may be further included, and the plurality of elastic pulling pieces 24 are respectively fixed on the plurality of annular supporting plates 22 in parallel; a plurality of protrusions 25 are respectively fixed on the end surfaces of the plurality of elastic pulling pieces 24 close to the core plate 11.
In some embodiments, the chip body 1 and the chip support 2 may be made of PMMA, PS, PC, PET, COC, or COP.
In some embodiments, each of the plurality of cell culture channels 111 may have a length of 1.5cm to 3.5cm, a width of 0.1mm to 2mm, and a depth of 0.5mm to 2mm.
The above description is only for the preferred embodiment of the present invention, and should not be construed as limiting the present invention, and any modifications, equivalent replacements, improvements, etc. made within the spirit and principle of the present invention should be included within the protection scope of the present invention.
Claims (10)
1. A 3D cell culture chip assembly, comprising: a chip body (1) and a chip bracket (2),
the chip body (1) comprises a core plate (11) and a plurality of liquid storage tanks (12), wherein a plurality of cell culture grooves (111) for culturing cells are formed in one plate surface of the core plate (11); the liquid storage tanks (12) are respectively fixed on the other plate surface of the core plate (11) corresponding to the two ends of the cell culture tanks (111), and culture solution inlets and outlets communicated with the liquid storage tanks (12) are formed at the bottoms of the cell culture tanks (111);
the chip support (2) is provided with a core plate mounting hole (211); the core plate (11) is detachably connected in the core plate mounting hole (211).
2. The 3D cell culture chip assembly according to claim 1, further comprising a plurality of liquid injection cylinders, wherein the plurality of liquid injection cylinders are respectively positioned inside the plurality of liquid storage pools (12) and are fixed on the bottom of the liquid storage pools (12) corresponding to the outer peripheral sides of the culture liquid inlet and outlet.
3. A 3D cell culture chip assembly according to claim 2, wherein a plurality of the reservoirs (12) are provided with pipette tip placement notches (121) radially outwardly along the inner wall of the reservoir (12) near the mouth thereof, and the pipette tip placement notches (121) extend through the mouth end face of the reservoirs (12).
4. A 3D cell culture chip assembly according to claim 2, wherein the plurality of reservoirs (12) are cylindrical in shape and have a height of: 5mm-15mm, the inner hole diameter is: 6mm-10mm; the height of a plurality of liquid injection cylinders is as follows: 3mm-5mm; the inner diameter of the liquid injection cylinders is 0.5-1.5mm.
5. The 3D cell culture chip assembly according to claim 1, further comprising a communication groove (14) and a plurality of blocking pillars (13), wherein the cell culture grooves (111) are symmetrically and radially arranged and communicated with each other corresponding to two ends of the communication groove (14); the blocking columns (13) are positioned in the communication groove (14) and are arranged into a plurality of strips at intervals, and the cell culture grooves (111) which are symmetrically arranged at two ends are communicated by the strips.
6. A3D cell culture chip assembly according to claim 5, wherein the cross section of the blocking pillars (13) is circular, triangular, quadrangular, pentagonal or teardrop-shaped, the height of the blocking pillars (13) is 0.1-2mm, and the interval between every two adjacent blocking pillars (13) is 0.1-0.5 mm.
7. The 3D cell culture chip assembly according to claim 1, wherein the chip holder (2) comprises a chip mounting plate (21), a plurality of annular support plates (22) and a plurality of elastic retaining buckles (23),
the other plate surface of the core plate (11) is provided with a mounting groove (112); a plurality of liquid storage tanks (12) are fixed at the bottoms of the mounting grooves (112); the chip mounting plate (21) is provided with a plurality of core plate mounting holes (211) penetrating through two plate surfaces of the chip mounting plate; the annular supporting plates (22) are close to one plate surface of the chip mounting plate (21) and are fixed on the inner wall of the core plate mounting hole (211) in a surrounding mode; the elastic limit buckles (23) are fixed at the middle parts of the hole walls of the core plate mounting holes (211) at intervals; the edge of one plate surface of the core plate (11) inserted into the core plate mounting hole (211) is abutted with the corresponding annular support plate (22), and the edge of the notch of the mounting groove (112) of the other plate surface is abutted with the elastic limit buckles (23).
8. A3D cell culture chip assembly according to claim 7, further comprising a plurality of resilient tabs (24) and a plurality of protrusions (25), wherein the plurality of resilient tabs (24) are respectively fixed in parallel on the plurality of annular support plates (22); the bulges (25) are respectively fixed on the end surfaces of the elastic shifting sheets (24) close to the core plate (11).
9. The 3D cell culture chip assembly according to claim 1, wherein the chip body (1) and the chip holder (2) are made of PMMA, PS, PC, PET, COC or COP.
10. A 3D cell culture chip assembly according to claim 1, wherein the plurality of cell culture channels (111) each have a length of 1.5cm to 3.5cm, a width of 0.1mm to 2mm and a depth of 0.5mm to 2mm.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202222019177.9U CN217868916U (en) | 2022-08-02 | 2022-08-02 | 3D cell culture chip subassembly |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202222019177.9U CN217868916U (en) | 2022-08-02 | 2022-08-02 | 3D cell culture chip subassembly |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN217868916U true CN217868916U (en) | 2022-11-22 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202222019177.9U Active CN217868916U (en) | 2022-08-02 | 2022-08-02 | 3D cell culture chip subassembly |
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| CN (1) | CN217868916U (en) |
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- 2022-08-02 CN CN202222019177.9U patent/CN217868916U/en active Active
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Effective date of registration: 20230817 Address after: No. 4 Jiangping Road, Xiannv Town, Jiangdu District, Yangzhou City, Jiangsu Province, 225200 Patentee after: Yangzhou Weiquan Core Intelligent Manufacturing Technology Co.,Ltd. Address before: 518000 unit 704, unit 3, building 1, dabaihui life and Health Industrial Park, 2028 Shenyan Road, Tiandong community, Haishan street, Yantian District, Shenzhen City, Guangdong Province Patentee before: Shenzhen Xirui Biotechnology Co.,Ltd. |