CN217809357U - Device for rapidly collecting aspergillus mycelium for extracting RNA (ribonucleic acid) through liquid fermentation - Google Patents
Device for rapidly collecting aspergillus mycelium for extracting RNA (ribonucleic acid) through liquid fermentation Download PDFInfo
- Publication number
- CN217809357U CN217809357U CN202221835109.3U CN202221835109U CN217809357U CN 217809357 U CN217809357 U CN 217809357U CN 202221835109 U CN202221835109 U CN 202221835109U CN 217809357 U CN217809357 U CN 217809357U
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- Prior art keywords
- circular plastic
- magnet
- plastic casing
- mycelium
- rigid coupling
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- Expired - Fee Related
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- 241000228212 Aspergillus Species 0.000 title claims abstract description 18
- 238000000855 fermentation Methods 0.000 title claims abstract description 16
- 230000004151 fermentation Effects 0.000 title claims abstract description 16
- 239000007788 liquid Substances 0.000 title claims abstract description 15
- 229920002477 rna polymer Polymers 0.000 title claims description 35
- 230000008878 coupling Effects 0.000 claims abstract description 15
- 238000010168 coupling process Methods 0.000 claims abstract description 15
- 238000005859 coupling reaction Methods 0.000 claims abstract description 15
- 230000005540 biological transmission Effects 0.000 claims abstract description 13
- 239000007787 solid Substances 0.000 claims abstract description 7
- 238000000605 extraction Methods 0.000 abstract description 12
- 238000004140 cleaning Methods 0.000 description 11
- 238000000034 method Methods 0.000 description 10
- 241000894006 Bacteria Species 0.000 description 4
- 241000233866 Fungi Species 0.000 description 4
- 238000002123 RNA extraction Methods 0.000 description 4
- 239000001963 growth medium Substances 0.000 description 4
- 239000007853 buffer solution Substances 0.000 description 3
- 238000004904 shortening Methods 0.000 description 3
- 239000000243 solution Substances 0.000 description 3
- 230000007547 defect Effects 0.000 description 2
- 230000002035 prolonged effect Effects 0.000 description 2
- 230000004075 alteration Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000005119 centrifugation Methods 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 239000011159 matrix material Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 239000002689 soil Substances 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 235000021419 vinegar Nutrition 0.000 description 1
- 239000000052 vinegar Substances 0.000 description 1
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- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The utility model discloses a device that aspergillus mycelium is used for drawing RNA is collected fast in liquid fermentation belongs to biological extraction technical field, including circular plastic casing, circular plastic casing's top surface is rotated and is connected with the cap, and the surface rigid coupling on circular plastic casing top has the dog, circular plastic casing's internal surface is hugged closely has embedded funnel, and the both ends of circular plastic casing surface are fixed the cup joint respectively and are had solid fixed ring and ring rail, be provided with magnet one between solid fixed ring and the ring rail, the both ends difference rigid coupling of magnet one has motor and slide bar, the other end rigid coupling of slide bar has the slide button, the rigid coupling has the transmission shaft on the output of motor. This draw device of RNA can shorten the collection time of mycelium to shorten the cycle that the RNA drawed, improved extraction efficiency, and can clear up circular plastic casing fast, not only improved clean efficiency, still can guarantee clean quality.
Description
Technical Field
The utility model relates to a biological extraction technical field specifically is a device that liquid fermentation collects aspergillus mycelium fast and is used for extracting RNA.
Background
Aspergillus, which is an important strain in the fermentation industry and the food processing industry, is utilized to nearly 60 kinds of strains which are widely distributed on grains, air, soil and various organic articles and mainly used for brewing wine and making vinegar, and mycelium, namely an aggregate of hyphae, is criss-cross, has various shapes and diversity, is spread and extended in a matrix or on a culture medium and repeatedly branches to form hypha groups;
the device of general extraction RNA on market can't collect aspergillus mycelium fast, make the collection time extension of mycelium, thereby the cycle of RNA extraction has been prolonged, extraction efficiency has been reduced, simultaneously can't completely cut off external miscellaneous fungus, make the RNA quality of extraction reduce, and the device of general extraction RNA can't clean the inner wall fast, cleaning efficiency has not only been reduced, still easily influence clean quality, the problem of device defect has been brought, for this, we propose a device that aspergillus mycelium is used for extracting RNA is collected fast in liquid fermentation.
SUMMERY OF THE UTILITY MODEL
An object of the utility model is to provide a device that aspergillus mycelium is used for extracting RNA is collected fast in liquid state fermentation, the device that generally draws RNA that provides in solving above-mentioned background art can't collect aspergillus mycelium fast, make the collecting time extension of mycelium, thereby the cycle that RNA drawed has been prolonged, the extraction efficiency has been reduced, simultaneously can't completely cut off external miscellaneous fungus, make the RNA quality of drawing reduce, and generally draw the device of RNA can't clean inner wall fast, cleaning efficiency has not only been reduced, still easily influence clean quality, the problem of device defect has been brought.
In order to achieve the above object, the utility model provides a following technical scheme: the utility model provides a device that aspergillus silk body is used for extracting RNA is collected fast in liquid state fermentation, includes circular plastic casing, the top surface of circular plastic casing rotates and is connected with the cap, and the surface rigid coupling on circular plastic casing top has the dog, the internal surface of circular plastic casing is hugged closely has embedded funnel, and the both ends of circular plastic casing surface are fixed the cup joint respectively and are had solid fixed ring and ring rail, gu be provided with magnet one between fixed ring and the ring rail, the both ends of magnet one rigid coupling respectively have motor and slide bar, the other end rigid coupling of slide bar has slide button, the rigid coupling has the transmission shaft on the output of motor, the other end fixed cup joint of transmission shaft has the gear, the surface mesh of gear is connected with annular rack, the internal surface sliding connection of circular plastic casing has the brush-holder, the opposite side rigid coupling of brush-holder has the carriage release lever, magnet two has been cup jointed to the carriage release lever internal fixation.
Preferably, the first magnet is slidably connected with the outer surface of the circular plastic shell, the top end of the sliding rod is slidably sleeved with the annular guide rail, and the sliding button is slidably connected with the top surface of the annular guide rail.
Preferably, the annular rack is fixedly connected with the top surface of the fixing ring, and the motor drives the magnet to move through the transmission shaft, the gear and the annular rack.
Preferably, the first magnet and the second magnet attract each other, and the moving rod and the brush rod are respectively and symmetrically arranged about a bisecting plane of the circular plastic shell.
Preferably, the diapire of embedded funnel has seted up the hole, the hole is provided with ten, and the diameter of hole is 1mm.
Preferably, the cross section of the stopper is arranged in an L shape, and the inner surface of the stopper is embedded and sleeved with the shell cover.
Compared with the prior art, the beneficial effects of the utility model are that:
the device for extracting the RNA seals the circular plastic shell by matching the baffle block with the shell cover, so that external sundry bacteria are prevented from entering the circular plastic shell, the RNA of sundry bacteria is prevented from being mixed into the RNA of the mycelium, the quality of the subsequently extracted RNA is ensured, the mycelium can be collected by lifting the embedded funnel, and the mycelium can be quickly washed by adding a buffer solution into the embedded funnel so as to remove a culture medium, the whole operation process can be finished within 1 minute, compared with a method for centrifugally collecting the mycelium and rinsing, the device can save 14 minutes, the purpose of shortening the collection time is achieved, the period of RNA extraction is shortened, and the extraction efficiency is improved;
this draw device of RNA drives gear revolve through the transmission shaft on the motor, and the annular rack who is connected with gear engagement is fixed, make the gear remove around the internal surface of annular rack, thereby indirectly drive motor and magnet one and slide, make magnet one rotate around the surface of circular plastic casing under slide bar and ring rail's direction, and magnet one and magnet inter attraction, make magnet two follow magnet one and remove, and then drive brush-holder stud and the carriage release lever slip above that, make the brush-holder stud scrape the internal surface of circular plastic casing, reach the purpose of clearing up circular plastic casing fast, cleaning efficiency has not only been improved, still can guarantee cleaning quality.
Drawings
FIG. 1 is a schematic view of the overall structure of the present invention;
FIG. 2 is an enlarged view of a portion A of FIG. 1;
FIG. 3 is an enlarged view of the portion B of FIG. 1;
fig. 4 is a cross-sectional view of the circular plastic casing of the present invention.
In the figure: 1. a circular plastic housing; 2. a shell cover; 3. a stopper; 4. a funnel is embedded; 5. a fixing ring; 6. an annular rack; 7. a gear; 8. a drive shaft; 9. a motor; 10. a first magnet; 11. a slide bar; 12. a slide button; 13. an annular guide rail; 14. a brush bar; 15. a travel bar; 16. and a second magnet.
Detailed Description
The technical solutions in the embodiments of the present invention will be described clearly and completely with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative work belong to the protection scope of the present invention.
Example 1:
referring to fig. 1, the present invention provides a technical solution: the utility model provides a device that aspergillus mycelium is used for extracting RNA is collected fast in liquid state fermentation, including circular plastic casing 1, the top surface of circular plastic casing 1 is rotated and is connected with cap 2, and the surface rigid coupling on 1 top of circular plastic casing has dog 3, the internal surface of circular plastic casing 1 is hugged closely has embedded funnel 4, and the both ends of 1 surface of circular plastic casing are fixed the cup jointing respectively and are had solid fixed ring 5 and ring rail 13, the hole has been seted up to embedded funnel 4's diapire, the hole is provided with ten, and the diameter of hole is 1mm, the transversal L type setting of personally submitting of dog 3, and the internal surface of dog 3 cup joints with 2 gomphosis of cap.
It is specific, collect the in-process of mycelium, rotate cap 2 on the circular plastic casing 1, make cap 2 and dog 3 separate, thereby expose embedded funnel 4 in the circular plastic casing 1, this in-process, accessible dog 3 cooperation cap 2 seals circular plastic casing 1, avoid external miscellaneous fungus to get into circular plastic casing 1, thereby the RNA of miscellaneous fungus has been avoided sneaking into in the RNA of mycelium, the RNA quality of follow-up extraction has been guaranteed, mention embedded funnel 4 again, can collect the mycelium, it can wash the mycelium to add the buffer solution in the embedded funnel 4 again fast, so as to get rid of the culture medium, whole operation process can be accomplished in 1 minute, for the method of centrifugation collection mycelium and rinsing, can practice thrift 14 minutes, reach the purpose of shortening the collection time, thereby the cycle of RNA extraction has been shortened, the extraction efficiency is improved.
Example 2:
referring to fig. 1-4, the present invention provides a technical solution: a device for rapidly collecting aspergillus filaments for extracting RNA through liquid fermentation is characterized in that a first magnet 10 is arranged between a fixed ring 5 and an annular guide rail 13, a motor 9 and a slide rod 11 are fixedly connected to two ends of the first magnet 10 respectively, a slide button 12 is fixedly connected to the other end of the slide rod 11, a transmission shaft 8 is fixedly connected to the output end of the motor 9, a gear 7 is fixedly sleeved at the other end of the transmission shaft 8, an annular rack 6 is connected to the outer surface of the gear 7 in a meshed mode, a brush rod 14 is slidably connected to the inner surface of a circular plastic shell 1, a moving rod 15 is fixedly connected to the other side of the brush rod 14, a second magnet 16 is fixedly sleeved in the moving rod 15, the first magnet 10 is slidably connected to the outer surface of the circular plastic shell 1, the top end of the slide rod 11 is slidably sleeved with the annular guide rail 13, the slide button 12 is slidably connected to the top surface of the annular guide rail 13, the annular rack 6 is fixedly connected to the top surface of the fixed ring 5, the motor 9 drives the first magnet 10 to move through the transmission shaft 8, the gear 7 and the annular rack 6, the first magnet 10 and the second magnet 16 attract each other, and the moving rod 15 and the brush rod 14 are symmetrically arranged about the bisecting plane of the circular plastic shell 1.
Specifically, in the process of cleaning the circular plastic shell 1, the motor 9 on the magnet one 10 is started, so that the transmission shaft 8 on the motor 9 drives the gear 7 to rotate, the annular rack 6 in meshed connection with the gear 7 is fixed, the gear 7 moves around the inner surface of the annular rack 6, the motor 9 and the magnet one 10 are indirectly driven to slide, the magnet one 10 rotates around the outer surface of the circular plastic shell 1 under the guidance of the sliding rod 11 and the annular guide rail 13, the magnet one 10 and the magnet two 16 attract each other, the magnet two 16 moves along with the magnet one 10, and the brush rod 14 and the moving rod 15 on the magnet one 16 are driven to slide, so that the brush rod 14 scrapes the inner surface of the circular plastic shell 1, the purpose of quickly cleaning the circular plastic shell 1 is achieved, the cleaning efficiency is improved, and the cleaning quality is guaranteed.
The working principle is as follows: when mycelium needs to be collected, the shell cover 2 on the circular plastic shell 1 is rotated, so that the shell cover 2 is separated from the stopper 3, the embedded funnel 4 in the circular plastic shell 1 is exposed, in the process, the circular plastic shell 1 can be sealed by matching the stopper 3 with the shell cover 2, external sundry bacteria are prevented from entering the circular plastic shell 1, RNA of sundry bacteria is prevented from being mixed into RNA of the mycelium, the quality of the subsequently extracted RNA is ensured, the mycelium can be collected by lifting the embedded funnel 4, the mycelium can be quickly washed by adding buffer solution into the embedded funnel 4, a culture medium is removed conveniently, the whole operation process can be finished in 1 minute, and compared with a method for centrifugally collecting the mycelium and rinsing, 14 minutes can be saved, the purpose of shortening the collection time is achieved, the cycle of RNA extraction is shortened, the extraction efficiency is improved, when the circular plastic shell 1 needs to be cleaned, the motor 9 on the magnet I10 is started, the transmission shaft 8 on the motor 9 drives the gear 7 to rotate, the annular rack 6 which is meshed with the gear 7 is fixed, the gear 7 moves around the inner surface of the annular rack 6, the motor 9 and the magnet I10 are indirectly driven to slide, the magnet I10 rotates around the outer surface of the circular plastic shell 1 under the guidance of the sliding rod 11 and the annular guide rail 13, the magnet I10 and the magnet II 16 attract each other, the magnet II 16 moves along with the magnet I10, the brush rod 14 and the moving rod 15 on the magnet I are driven to slide, the brush rod 14 scrapes the inner surface of the circular plastic shell 1, the purpose of quickly cleaning the circular plastic shell 1 is achieved, the cleaning efficiency is improved, the cleaning quality can be guaranteed, and the operation is completed.
Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.
Claims (6)
1. The utility model provides a device that liquid state fermentation collected aspergillus mycelium fast and is used for extracting RNA, includes circular plastic casing (1), its characterized in that: the top surface of circular plastic casing (1) rotates and is connected with cap (2), and the surface rigid coupling on circular plastic casing (1) top has dog (3), the internal surface of circular plastic casing (1) is hugged closely has embedded funnel (4), and the both ends of circular plastic casing (1) surface are fixed fixedly respectively and have been cup jointed solid fixed ring (5) and ring rail (13), be provided with magnet (10) between solid fixed ring (5) and ring rail (13), the both ends of magnet (10) are the rigid coupling respectively have motor (9) and slide bar (11), the other end rigid coupling of slide bar (11) has slide button (12), the rigid coupling has transmission shaft (8) on the output of motor (9), the other end fixed cup joints of transmission shaft (8) has gear (7), the surface meshing of gear (7) is connected with annular rack (6), the internal surface sliding connection of circular plastic casing (1) has brush-holder (14), the opposite side of brush-holder (14) has movable rod (15), movable rod (15) internal fixation has cup jointed magnet two (16).
2. The device for rapidly collecting aspergillus mycelium for extracting RNA (ribonucleic acid) by liquid fermentation as claimed in claim 1, wherein: the magnet I (10) is connected with the outer surface of the circular plastic shell (1) in a sliding mode, the top end of the sliding rod (11) is connected with the annular guide rail (13) in a sliding sleeved mode, and the sliding button (12) is connected with the top face of the annular guide rail (13) in a sliding mode.
3. The device for rapidly collecting aspergillus mycelium for extracting RNA through liquid fermentation according to claim 1, is characterized in that: the top surface rigid coupling of annular rack (6) and solid fixed ring (5), motor (9) drive magnet (10) through transmission shaft (8), gear (7) and annular rack (6) and remove.
4. The device for rapidly collecting aspergillus mycelium for extracting RNA through liquid fermentation according to claim 1, is characterized in that: the first magnet (10) and the second magnet (16) attract each other, and the moving rod (15) and the brush rod (14) are respectively arranged symmetrically relative to a bisecting plane of the circular plastic shell (1).
5. The device for rapidly collecting aspergillus mycelium for extracting RNA through liquid fermentation according to claim 1, is characterized in that: the bottom wall of the embedded funnel (4) is provided with ten holes, and the diameter of each hole is 1mm.
6. The device for rapidly collecting aspergillus mycelium for extracting RNA through liquid fermentation according to claim 1, is characterized in that: the cross section of the stop block (3) is arranged in an L shape, and the inner surface of the stop block (3) is embedded and sleeved with the shell cover (2).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202221835109.3U CN217809357U (en) | 2022-07-18 | 2022-07-18 | Device for rapidly collecting aspergillus mycelium for extracting RNA (ribonucleic acid) through liquid fermentation |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202221835109.3U CN217809357U (en) | 2022-07-18 | 2022-07-18 | Device for rapidly collecting aspergillus mycelium for extracting RNA (ribonucleic acid) through liquid fermentation |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN217809357U true CN217809357U (en) | 2022-11-15 |
Family
ID=83966777
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202221835109.3U Expired - Fee Related CN217809357U (en) | 2022-07-18 | 2022-07-18 | Device for rapidly collecting aspergillus mycelium for extracting RNA (ribonucleic acid) through liquid fermentation |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN217809357U (en) |
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2022
- 2022-07-18 CN CN202221835109.3U patent/CN217809357U/en not_active Expired - Fee Related
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20221115 |