CN217786685U - Sample preparation device for cytokine - Google Patents

Abstract

The utility model relates to a cytokine sample preparation technical field, in particular to cytokine's sample preparation facilities, including the automatic sample injector subassembly of the sample that awaits measuring, the sample transmission subassembly that awaits measuring, sample reaction vessel subassembly, cytokine reagent transmission subassembly, diluent transmission subassembly, sample mixing subassembly, magnetic separation needle subassembly and magnetic separation magnet subassembly. Compared with the prior art, the utility model discloses a quality at the reaction sequence of sample and reagent is guaranteed to cytokine's sample preparation facilities, avoids any artificial intervention completely, eliminates operating personnel to expose in the biological risk of the easy infection of contact high risk. An operator only needs to put a sample container to be detected into a sample container rack to be detected, place the sample container rack on an automatic sample injector assembly of the sample to be detected, obtain a prepared sample to be detected according to a set flow, and then perform flow analysis and detection.

Description

Sample preparation device for cytokine

[ technical field ] A method for producing a semiconductor device

The utility model relates to a cytokine sample preparation technical field, in particular to cytokine's sample preparation facilities.

[ background ] A method for producing a semiconductor device

Flow Cytometry (FCM) is a flow cytometer that is used to count and quantify various biological and physical and biochemical characteristics of blood, various body fluids, bone marrow, biopsy materials, single cell suspensions of animals and plants, paraffin-embedded tissues, including cells, platelets, organelles, sperm, microorganisms, and artificially synthesized microspheres, etc.

With the progress and popularization of scientific technology, flow analyzers gradually move into the field of medical clinical application, and the current flow analyzer technology plays an important role in the assessment of human cellular immune function and the diagnosis and treatment of various blood diseases and tumors, and plays a key role in the diagnosis, disease condition monitoring, prognosis judgment and medication opportunity assessment of diseases such as AIDS, leukemia and tumors.

Before the flow analysis detection is carried out, necessary processing and preparation must be carried out on a sample to be detected, wherein the quality of the preparation of the sample to be detected directly influences the effectiveness and accuracy of data detection.

At present, a sample to be measured of a flow analyzer is usually processed by a human before measurement, and the preparation process is very complicated and roughly as follows:

firstly adding a sample to be detected into a flow tube (sample reaction container), then manually sucking magnetic beads (reagent B) into the flow tube, holding the flow tube by hand, putting the flow tube on a vortex mixing instrument for mixing, putting the flow tube into a dark box for carrying out first reaction incubation, and carrying out first magnetic separation after the incubation is finished to suck supernatant; and manually adding a labeled antibody (reagent A), putting the hand-held flow type tube on a vortex instrument, mixing uniformly again, putting the hand-held flow type tube in a dark box for carrying out secondary reaction incubation, carrying out secondary magnetic separation after incubation is finished, removing supernatant, manually adding a magnetic bead diluent, mixing uniformly, carrying out magnetic separation again, removing supernatant, adding the magnetic bead diluent, mixing uniformly, and finishing the preparation of the sample.

Manual operation, from uncapping the sample, to carrying out magnetic separation and absorbing supernatant etc. the sample exposes in the air, and the operator especially is the quick-witted infectious sample of accuse center, and the frequent contact high risk is easy, and when vortex mixing, the liquid level vibrates and can lead to the sample to splash especially, and the operator has high biological risk.

The manual operation steps are complicated and error is easy to occur, and different operators have inconsistent use habits on operating pipettors and mixing vortex mixing instruments, so that the risk of inconsistent flow detection results is often caused.

In summary, the existing manual sample preparation method has the defects of susceptibility to infection of operators, complicated steps, poor result caused by inconsistent habits of the operators, easy fatigue of the operators when a large number of samples are processed and the like, and particularly, the flow-type detection result is inaccurate due to lack of operation experience or unskilled performance of the operators in small and medium hospitals or institutions on manual sample preparation, so the existing manual sample preparation needs to be improved.

[ Utility model ] A method for manufacturing a semiconductor device

In order to overcome the above problems, the present invention provides a device for preparing cytokine samples, which can effectively solve the above problems.

The utility model provides an above-mentioned technical problem provide a technical scheme is: the device comprises an automatic sample injector component for a sample to be detected, a sample transmission component for the sample to be detected, a sample reaction container component, a cytokine reagent transmission component, a diluent transmission component, a sample blending component, a magnetic separation needle component and a magnetic separation magnet component; the sample reaction container assembly is positioned at the rear side of the sample autosampler assembly to be detected, and the sample transmission assembly to be detected is positioned above the sample autosampler assembly to be detected and the sample reaction container assembly; the cytokine reagent assembly is positioned at one side of the sample reaction container assembly, and the cytokine reagent transmission assembly is positioned behind the cytokine reagent assembly and the sample reaction container assembly; the diluent assembly is positioned on one side of the sample reaction container assembly, the diluent transmission assembly is positioned on one side of the diluent assembly, and the sample blending assembly is positioned on one side of the sample reaction container assembly; the magnetic separation needle assembly is arranged above the sample reaction container assembly, and the magnetic separation magnet assembly is arranged below the sample reaction container assembly and used for magnetic separation.

Preferably, the magnetic separation needle assembly comprises a magnetic separation needle and a needle driving assembly, and the magnetic separation needle is driven by the needle driving assembly to move up and down; the magnetic separation magnet assembly comprises a magnetic separation magnet and a magnet driving assembly, wherein the magnetic separation magnet rotates under the action of the magnet driving assembly, so that one corner of the magnetic separation magnet contacts the bottom of a sample tube in the sample reaction container assembly to adsorb target magnetic microspheres.

Preferably, be provided with on the sample auto-sampler subassembly that awaits measuring that there is or not identification position, sample to be measured suction position and the Y to the sensor that targets in place of bar code scanning and sample container to be measured, the Y is to the sensor that targets in place setting in the inboard edge of sample auto-sampler subassembly that awaits measuring, and bar code scanning and sample container to be measured have or not identification position to set up in Y to the sensor left side that targets in place, and sample to be measured suction position sets up in bar code scanning and sample container to be measured have or not identification position left side.

Preferably, the diluent assembly includes a diluent container a and a diluent rotary gate, the diluent container a being hung on the diluent rotary gate, the diluent rotary gate being located at one side of the sample reaction container assembly.

Preferably, the diluent transfer assembly comprises a transfer pump a and a diluent transfer needle, which are located behind the sample reaction vessel assembly.

Preferably, the sample reaction vessel assembly comprises a sample reaction vessel carrier mechanism and a two-dimensional movement mechanism, the sample reaction vessel carrier mechanism being provided on the two-dimensional movement mechanism.

Preferably, the two-dimensional movement mechanism comprises a rotary driving mechanism and a horizontal driving mechanism, the sample reaction vessel bracket mechanism is arranged on the rotary driving mechanism, and the rotary driving mechanism is arranged on the horizontal driving mechanism.

Preferably, the cytokine reagent assembly is provided with a cytokine reagent storage container bracket, a rack and a movement mechanism, the cytokine reagent storage container bracket is arranged on the movement mechanism, the cytokine reagent storage container bracket is provided with horizontally arranged slotted holes, and a cytokine reagent kit is placed in the slotted holes; the cytokine kit comprises at least two storage containers, microspheres are stored in the storage containers on the outer sides of the storage containers, a pair of fins is arranged at the bottoms of the storage containers, the lower ends of the storage containers are connected with gears, and the gears are in clutch connection with racks.

Preferably, the sample mixing subassembly includes mixing rotary driving mechanism, the vertical actuating mechanism of mixing jack-up, mixing top, diluent transmission needle and diluent needle belt cleaning device, mixing rotary driving mechanism locates on the vertical actuating mechanism of mixing jack-up, the mixing top is located on the mixing rotary driving mechanism, diluent needle belt cleaning device is located mixing top.

Preferably, the diluent needle cleaning device is sleeved on the diluent transmission needle, and the diluent transmission needle is fixed on the flow type sample blending assembly; the diluent transmission needle, the diluent needle cleaning device and the blending ejector are located on the same axis, and the diluent needle cleaning device and the blending ejector are installed on the same guide rail.

Compared with the prior art, the utility model discloses a cytokine's sample preparation facilities can realize carrying out the mixing to the sample that awaits measuring automatically, acquire the sample that awaits measuring from the sample container that awaits measuring automatically, the sample that will await measuring adds the sample reaction vessel that awaits measuring automatically, and automatic required multiple STREAMING reagent that adds, carry out the mixing to the mixture of sample and various antibody reagents that awaits measuring automatically, guarantee the quality at the reaction process of sample and reagent, avoid any manual intervention completely, eliminate operating personnel and expose the biological risk of the easy infection of contact high risk. An operator only needs to put a sample container to be detected into a sample container rack to be detected, place the sample container rack on an automatic sample injector assembly of the sample to be detected, obtain a prepared sample to be detected according to a set flow, and then perform flow analysis and detection.

[ description of the drawings ]

FIG. 1 is a perspective view of a cytokine sample preparation device of the present invention;

FIG. 2 is a schematic plan view of the cytokine sample preparation device of the present invention;

FIG. 3 is a plan view of the sample injector assembly for cytokine samples according to the present invention;

FIG. 4 is a schematic diagram of a sample transport assembly of the cytokine sample preparation device of the present invention;

FIG. 5 is a schematic view of a sample reaction vessel assembly of the cytokine sample preparation device of the present invention;

FIG. 6 is a schematic view of a cytokine reagent delivery assembly of the cytokine sample preparation device of the present invention;

FIG. 7 is a schematic diagram of a cytokine sample mixing assembly of the cytokine sample preparation device of the present invention;

FIG. 8 is a schematic view of the cytokine reagent mixing assembly of the cytokine sample preparation device of the present invention;

FIG. 9 is a schematic view of a magnetic cytokine separation needle assembly of the cytokine sample preparation device of the present invention;

FIG. 10 is a schematic view of the magnetic cytokine separating magnet assembly of the device for preparing cytokine samples according to the present invention;

reference is made to the accompanying drawings in which:

100-an autosampler assembly of a sample to be measured;

110-sample container rack to be tested;

120-sample container to be tested;

130-bar code scanning and the existence of identification positions of the sample container to be detected;

140-a sample suction position to be measured;

150-Y-direction in-position sensor;

200-a sample reaction vessel assembly;

201-horizontal movement trajectory of sample reaction vessel rack;

202-placing and taking a sample reaction container;

203-sample reaction vessel rack working position;

204-the rotation direction of the working position of the sample reaction vessel frame;

205-adding a sample to be detected into a reaction container;

206-cytokine reagent needle application position;

207-mixing in a reaction container, and adding a dilution liquid level;

208-sample reaction vessel;

209-magnetic separation position;

210-a sample reaction vessel assembly rotation mechanism;

220-sample reaction vessel assembly horizontal mechanism;

230-sample reaction vessel carrier mechanism;

240-sample reaction vessel rack position detection optocoupler;

250-sample reaction vessel rack;

300-a cytokine reagent component;

301-direction of movement of cytokine reagent assembly;

302-reagent B uptake site;

303-cytokine reagent storage container;

304-cytokine reagent storage container holder;

305 — reagent a uptake site;

306-kit;

307-a kit cup holder;

308-a cassette-in-place encoder;

309-cartridge-in-place motor;

310-longitudinal cartridge guide rails;

311, uniformly mixing a kit rack;

312 — reagent component holding plate;

401-cleaning station for sample needle

402-reagent needle cleaning station

500-a cytokine reagent delivery assembly;

501-reagent needle rotation track;

510-rocker arm lifting mechanism;

511-rocker arm lifting mechanism motor;

520-a rocker arm rotation mechanism;

521-a rocker arm rotation mechanism motor;

530-cytokine reagent needle;

600-a sample blending assembly;

610-a sample blending rotating motor;

620-sample mixing lifting motor;

630-sample mixing ejector rod;

640-diluent delivery needle;

650-diluent needle cleaning device;

700-a sample to be tested transport assembly;

710-a motor before and after the sample to be tested is transmitted;

720-a sample suction assembly to be tested;

721, a sample needle lifting motor for sucking and spitting a sample to be detected;

722-sample needle up-down slide block seat for sucking and spitting sample to be measured;

723-sample needle for sucking and spitting sample to be measured;

800-diluent revolving door;

810-diluent container a;

900-transfer pump;

910 — transfer pump a;

1010-a magnetic separation needle assembly;

1011-mounting plate of magnetic separation needle assembly;

1012-magnetic separation needle mounting;

1013-magnetic separation needle;

1014-magnetic separation needle motor;

1020-a magnetic separation magnet assembly;

1021-a magnetically separated magnet assembly mounting plate;

1022-a magnetically split magnet motor;

1023-magnetic separation magnet fixing piece;

1024 — magnetic separation magnet.

[ detailed description ] embodiments

In order to make the objects, technical solutions and advantages of the present invention more clearly understood, the present invention will be further described in detail with reference to the accompanying drawings and the following embodiments. It should be understood that the specific embodiments described herein are for purposes of illustration only and are not intended to limit the invention.

It should be noted that all directional indications (such as up, down, left, right, front, back, \8230;) in embodiments of the invention are limited to relative positions on a given view, not absolute positions.

In addition, descriptions in the present application as to "first", "second", and the like are for descriptive purposes only and are not to be construed as indicating or implying relative importance or implicit to the number of technical features indicated. Thus, a feature defined as "first" or "second" may explicitly or implicitly include at least one of the feature. In the description of the present invention, "a plurality" means at least two, e.g., two, three, etc., unless explicitly defined otherwise.

Referring to fig. 1 and 2, the device for preparing a cytokine sample according to the present invention includes an auto sampler assembly 100 for a sample to be tested, a sample transfer assembly 700 for a sample to be tested, a sample reaction container assembly 200, a cytokine reagent assembly 300, a cytokine reagent transfer assembly 500, a diluent assembly, a diluent transfer assembly, a sample mixing assembly 600, a magnetic separation needle assembly 1010, and a magnetic separation magnet assembly 1020.

The sample autosampler subassembly 100 that awaits measuring locates the front side, sample reaction vessel subassembly 200 is located the sample autosampler subassembly 100 rear side that awaits measuring, the sample transmission subassembly 700 that awaits measuring is located the sample autosampler subassembly 100 that awaits measuring and sample reaction vessel subassembly 200 top.

The cytokine reagent assembly 300 is positioned at one side of the sample reaction vessel assembly 200, and the cytokine reagent transfer assembly 500 is positioned behind the cytokine reagent assembly 300 and the sample reaction vessel assembly 200. The sample transport assembly 700 is used to add the sample to be tested in the sample autosampler assembly 100 to the sample reaction vessel assembly 200. The cytokine reagent delivery assembly 500 is used to add cytokine reagent a and reagent B of the cytokine reagent assembly 300 to the sample reaction vessel assembly 200. The diluent assembly is located at one side of the sample reaction vessel assembly 200, the diluent transmission assembly is located at one side of the diluent assembly, and the diluent assembly is used for adding the diluent in the diluent assembly into the sample reaction vessel assembly 200. The sample mixing assembly 600 is located at one side of the sample reaction container assembly 200, and the sample mixing assembly 600 is used for uniformly mixing the added sample to be tested and the cytokine reagent a in the cytokine sample reaction container assembly, or the added sample to be tested and the cytokine reagent B, and the diluent. The magnetic separation needle assembly 1010 is disposed above the sample reaction container assembly 200, and the magnetic separation magnet assembly 1020 is disposed below the sample reaction container assembly 200 for magnetic separation.

The diluent assembly includes a diluent container a810 and a diluent rotary gate 800, the diluent container a810 is hung on the diluent rotary gate 800, and the diluent rotary gate 800 is located at one side of the sample reaction container assembly 200.

The diluent transfer assembly includes a transfer pump a910 and a diluent transfer needle 640, and the transfer pump a910 and the diluent transfer needle 640 are located behind the sample reaction vessel assembly 200.

Referring to fig. 3, a sample container rack 110 to be tested is placed on the automatic sample injector assembly 100, a sample container 120 to be tested is placed on the sample container rack 110, and a sample to be tested is placed in the sample container 120. The sample container 120 to be tested is an unclosed blood collection tube.

One or more sample container holders 110 to be tested are placed on the auto-sampler component 100, and the sample container holders 110 to be tested move on the auto-sampler component 100 to be tested and enter from the right side and exit from the left side. One or more sample containers 120 to be tested are placed on the sample container rack 110. The automatic sample injector assembly 100 for sample to be measured moves the sample container rack 110 to be measured in the Y direction first, and then moves in the X direction. The Y-direction motion is a translation of the entire gantry along the Y-direction. During the movement along the X direction, the sample transfer assembly 700 is adapted to suck the sample to be measured by moving the position of the sample container 120 to be measured one at a time.

The automatic sample injector component 100 for the samples to be detected is provided with a bar code scanning and sample container to be detected with or without identification positions 130, a sample suction position 140 for the samples to be detected and a Y-direction in-place sensor 150, the Y-direction in-place sensor 150 is arranged at the inner side edge of the automatic sample injector component 100 for the samples to be detected, the bar code scanning and sample container to be detected with or without identification positions 130 are arranged at the left side of the Y-direction in-place sensor 150, and the sample suction position 140 for the samples to be detected is arranged at the left side of the bar code scanning and sample container to be detected with or without identification positions 130. After the sample container rack 110 to be tested moves to the inner side along the Y direction, the Y-direction in-place sensor 150 is triggered, and the sample container rack 110 to be tested moves along the X direction, and moves one position of the sample container 120 to be tested along the X direction each time. Each sample container 120 is moved to the barcode scanning and identification position 130 for detecting the presence or absence of test tubes and barcode scanning. The sample container rack 110 then moves a position of the sample container 120 along the X direction to the sample suction position 140, where the sample transport assembly 700 samples the sample container 120. Each sample container 120 to be tested is driven by the sample container rack 110 to be tested to sequentially pass through the barcode scanning, the sample container to be tested identification position 130 and the sample suction position 140. A sample container rack pusher (not shown) is disposed on the left side of the to-be-tested sample autosampler assembly 100, the to-be-tested sample container rack 110 moves to the left side of the to-be-tested sample autosampler assembly 100 along the X direction, the to-be-tested sample container rack 110 is driven to leave the X direction by the rotation of the to-be-tested sample container rack pusher (not shown) along the R direction, and the to-be-tested sample container rack pusher (not shown) resets along the R direction after each ejection of one to-be-tested sample container rack 110. The sample container 120 moves from the barcode scanning and sample container presence/absence identification position 130 to the sample suction position 140 after moving one sample container 120 position in the X direction.

Referring to fig. 4, the sample transmission assembly 700 includes a two-dimensional beam, the two-dimensional beam is provided with a sample absorption assembly 720 to be detected and a sample transmission front-back motor 710 to be detected, and the sample transmission front-back motor 710 drives the sample absorption assembly 720 to be detected to move back and forth along the two-dimensional beam through a synchronous wheel and a synchronous belt. The sample sucking component 720 is used for sucking a sample.

The sample sucking and spitting device comprises a sample sucking and spitting component 720 to be detected, and is characterized in that a sample needle up-and-down sliding block seat 722 for sucking and spitting a sample to be detected is arranged on the sample sucking and spitting component 720 to be detected in a sliding mode, the sample needle up-and-down sliding block seat 722 for sucking and spitting the sample to be detected can slide up and down on the sample sucking and spitting component 720 to be detected, a sample needle 723 for sucking and spitting the sample to be detected is fixed on the sample needle up-and-down sliding block seat 722 for sucking and spitting the sample to be detected, a transmission pump 900 is arranged on one side of the transmission pump A910, and the sample needle 723 for sucking and spitting the sample to be detected is connected with the transmission pump 900 through a pipeline. The sample sucking and spitting assembly 720 is provided with a sample needle lifting motor 721 for sucking and spitting a sample to be detected, and the sample needle lifting motor 721 for sucking and spitting the sample to be detected drives the sample needle upper and lower slide block seat 722 for sucking and spitting the sample to be detected to slide up and down.

In operation, the sample sucking assembly 720 is driven by the motor 710 before and after the sample is transferred to move the sample sucking and spitting needle 723 for the sample to be tested to a position directly above the sample sucking site 140. The sample needle lifting motor 721 for sample sucking and spitting drives the sample needle upper and lower slider seat 722 for sample sucking and spitting to move downwards, the sample needle upper and lower slider seat 722 for sample sucking and spitting to be detected drives the sample needle 723 for sample sucking and spitting to enter the container of the sample container 120 to be detected, the sample needle 723 for sample sucking and spitting to be detected runs below the liquid level of the sample container 120 to be detected, and the transfer pump 900 connected with the sample needle 723 for sample sucking and spitting to be detected sucks the sample in the sample container 120 to be detected. After the sample needle 723 for sucking and spitting a sample to be detected runs to the upper position, the sample needle 723 for sucking and spitting a sample to be detected is driven by the motor 710 before and after the sample to be detected is transmitted, so that the sample needle 723 for sucking and spitting a sample to be detected moves to a position right above the sample adding reaction container position 205 of the sample reaction container assembly 200 (as shown in fig. 2). The sample needle 723 for sucking and spitting the sample to be detected is driven to move downwards by the sample needle lifting motor 721 for sucking and spitting the sample to be detected, and the transmission pump 900 connected with the sample needle 723 for sucking and spitting the sample to be detected transmits the sample to be detected to the flow type sample reaction container 208 on the sample adding reaction container position 205.

Referring to fig. 5, the sample reaction vessel assembly 200 includes a sample reaction vessel holder mechanism 230 and a two-dimensional movement mechanism, and the sample reaction vessel holder mechanism 230 is disposed on the two-dimensional movement mechanism. The two-dimensional movement mechanism comprises a rotary driving mechanism 210 and a horizontal driving mechanism 220, the sample reaction vessel bracket mechanism 230 is arranged on the rotary driving mechanism 210, and the rotary driving mechanism 210 is arranged on the horizontal driving mechanism 220.

The sample reaction container bracket mechanism 230 is provided with a sample reaction container structure, the sample reaction container structure is provided with a sample reaction container rack 250, the sample reaction container rack 250 is provided with a plurality of annularly arranged holes, and the plurality of annularly arranged holes can be inserted into the sample reaction containers 208.

During operation, the horizontal driving mechanism 220 drives the rotary driving mechanism 210 to move left and right. As shown in fig. 2, the sample reaction vessel rack 250 is placed at the sample reaction vessel rack placing position 202, and moves along the sample reaction vessel rack horizontal movement track 201 to the sample reaction vessel rack operating position 203.

Referring to fig. 2 and 5, the rotation driving mechanism 210 drives the sample reaction vessel rack 250 disposed thereon to rotate along the rotation direction 204 of the working position of the sample reaction vessel rack. The sample reaction vessel bracket mechanism 230 is further provided with a sample reaction vessel bracket position detection optocoupler 240 for detecting the movement position of the sample reaction vessel bracket 250.

Referring to fig. 2, the sample reaction vessel rack 250 has four working positions when in the working position 203: a sample to be tested is added into the reaction container 205, a cytokine reagent needle adding position 206, a magnetic separation position 209 and a reaction container mixing and diluting liquid level 207, and the sample reaction container 208 to be tested at any position of the sample reaction container rack 250 along the working position rotation direction 204 of the sample reaction container rack can be switched among four stations.

Referring to fig. 1 and 2, the cytokine reagent assembly 300 is provided with a cytokine reagent storage container bracket 304, a rack, and a moving mechanism, the cytokine reagent storage container bracket 304 is provided on the moving mechanism, the cytokine reagent storage container bracket 304 is provided with horizontally arranged slots, and the cytokine reagent kit 306 is placed in the slots. The cytokine kit 306 has at least two storage containers 303, the microspheres are stored in the outer storage container 303, the bottom of the container is provided with a pair of fins, the lower end of the container is connected with a gear, and the gear is in clutch with a rack. The reagent kit 306 reciprocates back and forth under the action of the motion mechanism, the rack is fixed, the gear rotates forwards and backwards, and the microspheres are mixed uniformly under the action of the bottom fins of the container. The reagent boxes 306 are under the action of the motion mechanism, so that the reagent in each reagent box has a unique reagent sucking position.

The cytokine reagent assembly 300 is provided with a cytokine reagent needle aspiration site, which is a reagent B aspiration site 302 and a reagent a aspiration site 305. The moving mechanism adopts a linear reciprocating mechanism, the cytokine reagent storage container bracket 304 reciprocates according to the moving direction 301 of the cytokine reagent assembly in fig. 2, and the cytokine reagent storage container 303 placed in any arrangement of slots can move to the reagent B suction position 302 or the reagent a suction position 305. Reagent a in cytokine reagent reservoir 303 is an antibody reagent and reagent B is an encoded microsphere reagent.

The cytokine reagent assembly 300 is further provided with a temperature control mechanism for controlling the temperature of the reagent a and the reagent B in the cytokine reagent storage container 303 so as to ensure the long-term activity of the reagents.

Referring to fig. 8, the cytokine reagent assembly 300 includes a reagent kit 306, a reagent kit cup holder 307, a reagent kit in-place encoder 308, a reagent kit in-place motor 309, a reagent kit longitudinal guide rail 310, a reagent kit blending rack 311 and a reagent kit fixing plate 312, wherein the reagent kit cup holder 307 is mounted on the reagent kit longitudinal guide rail 310, the guide rail 310 is mounted on the reagent kit fixing plate 312, the reagent kit in-place motor 309 and the reagent kit in-place encoder 308 are mounted on the reagent kit fixing plate 312, and the reagent kit in-place motor 309 can drive the reagent kit cup holder 307 to reciprocate longitudinally along the reagent kit longitudinal guide rail 310; the reagent box mixing rack 311 is arranged on the reagent box fixing plate 312, the reagent box 306 is arranged on the reagent box cup holder 307, a mixing gear is arranged at the reagent box magnetic bead position and meshed with the reagent box mixing rack 311, and when the reagent box in-place motor 309 drives the reagent box cup holder 307 to do longitudinal reciprocating motion along the reagent box longitudinal guide rail 310, the reagent box mixing rack 311 drives the reagent box magnetic bead position to rotate and mix uniformly. When taking the reagent, the reagent box in-place motor 309 drives the reagent box 306 in place, and the reagent box in-place encoder 308 feeds back to ensure that the reagent box is in place and accurate.

Referring to fig. 6, the cytokine reagent transfer assembly 500 includes a cytokine reagent needle 530, a rocker arm lifting mechanism 510 and a rocker arm rotating mechanism 520, wherein the rocker arm lifting mechanism 510 and the rocker arm rotating mechanism 520 form a three-dimensional movement mechanism in the vertical and horizontal directions, and the cytokine reagent needle 530 is connected to the transfer pump 900 through a pipe. The rocker arm rotating mechanism 520 and the rocker arm lifting mechanism 510 are arranged on the frame of the cytokine reagent transmission assembly 500, the cytokine reagent transmission assembly 500 further comprises a rocker arm lifting mechanism motor 511 and a rocker arm rotating mechanism motor 521, the rocker arm lifting mechanism motor 511 drives the rocker arm lifting mechanism 510 to lift, and the rocker arm rotating mechanism motor 521 drives the rocker arm rotating mechanism 520 to rotate. The cytokine reagent needle 530 is provided on the swing arm rotating mechanism 520, and the cytokine reagent needle 530 can be simultaneously lifted and rotated.

Referring to fig. 2, in operation, the cytokine reagent needle 530 may move along the reagent needle rotation track 501, the cytokine reagent needle 530 rotates from the reagent needle cleaning position 402 (initial position) to the reagent B aspiration position 302 or the reagent a aspiration position 305, and then moves downward, the cytokine reagent needle 530 enters the cytokine reagent storage container 303 to perform liquid level detection, the cytokine reagent needle 530 is lowered below the liquid level, and the cytokine reagent a or the reagent B is aspirated by the transfer pump 900 under negative pressure. Cytokine reagent needle 530 moves upward and then rotates to cytokine reagent needle loading position 206, cytokine reagent needle 530 moves downward, and the reagent needle approaches the bottom of sample reaction container 208 to be tested, and cytokine reagent a or reagent B is transferred into sample reaction container 208 to be tested by transfer pump 900. The cytokine reagent needle 530 moves upward and then rotates to the reagent needle cleaning position 402, and the cytokine reagent needle 530 moves downward and enters the cleaning tank to be cleaned and dried. The cytokine reagent needle 530 moves upward to be restored to the original position. Adding the sample to be detected at the position 205 of the sample adding reaction container, adding the cytokine reagent B at the position 206 of the cytokine reagent needle, rotating to the reaction container, mixing uniformly, and adding the dilution liquid level 207.

Referring to fig. 7, the sample blending assembly 600 includes a blending rotation driving mechanism 610, a blending jacking vertical driving mechanism 620, a blending jack 630, a diluent transmission needle 640, and a diluent needle cleaning device 650, wherein the blending rotation driving mechanism 610 is disposed on the blending jacking vertical driving mechanism 620, the blending jack 630 is disposed on the blending rotation driving mechanism 610, and the diluent needle cleaning device 650 is disposed above the blending jack 630 and has a spring to pull the diluent needle cleaning device 650 downward. The diluent needle cleaning device 650 is sleeved on the diluent transmission needle 640, and the diluent transmission needle 640 is fixed on the frame of the flow sample mixing assembly 600. Diluent transmission needle 640, diluent needle belt cleaning device 650 and mixing top 630 are located same axis, diluent needle belt cleaning device 650 and mixing top 630 are installed on same root guide rail, can follow same root guide rail up-and-down motion.

Referring to fig. 2 and fig. 7, in operation, the mixing ejector 630 ejects the sample reaction container 208 located at the position of the mixing and diluting liquid level 207 of the reaction container, the diluent needle cleaning device 650 clamps the upper opening of the sample reaction container 208, the sample reaction container 208 to be tested is ejected out of the sample reaction container holder 250, the sample reaction container 208 is clamped between the diluent needle cleaning device 650 and the mixing ejector 630, and the mixing rotation driving mechanism 610 drives the sample reaction container 208 to rotate and mix the sample to be tested and the cytokine reagent B. After completion of the mixing, the mixing tip 630 moves downward, and the mixing tip 630 and the diluent needle cleaning device 650 clamp the sample reaction container 208 and return to the sample reaction container rack 250. After the sample to be tested and the cytokine reagent B are added and mixed uniformly in sequence, the sample reaction container 208 containing the mixture of the sample to be tested and the cytokine reagent B is placed still on the sample reaction container rack 250 for incubation.

The magnetic separation needle assembly 1010 comprises a magnetic separation needle 1013 and a needle driving assembly, wherein the magnetic separation needle 1013 is driven by the needle driving assembly to move up and down; the magnetic separation magnet assembly 1020 comprises a magnetic separation magnet 1024 and a magnet driving assembly, wherein the magnetic separation magnet 1024 rotates under the action of the magnet driving assembly, so that one corner of the magnetic separation magnet 1024 contacts the bottom of a sample tube in the sample reaction container assembly 200 to adsorb target magnetic microspheres. The magnet separation needle 1013 contacts the liquid level, and the magnetic separation needle 1013 sucks the liquid in the sample tube in the process of descending until the needle contacts the bottom of the sample tube, and the needle sucks up all the liquid, and the magnetic separation needle 1013 moves up and out of the sample tube under the action of the needle driving component. The magnetic separation magnet 1024 rotates around the fixed shaft under the action of the magnet driving assembly, so that the magnetic separation magnet 1024 rotates back to the default initial position (the position where the magnet is parallel to the sample tube).

Referring to fig. 9, the magnetic separation needle assembly 1010 includes a magnetic separation needle assembly mounting plate 1011, a magnetic separation needle mounting member 1012, a magnetic separation needle 1013, and a magnetic separation needle motor 1014, forming a one-dimensional movement mechanism in a vertical direction. The magnetic separation needle mounting piece 1012 is arranged on a vertical guide rail slide block of a magnetic separation needle assembly mounting plate 1011; the magnetic separation needle 1013 is installed on the magnetic separation needle installation part 1012, and the magnetic separation needle 1013 can be driven by the magnetic separation needle motor 1014 to move up and down to perform the downward liquid-drawing action of the magnetic separation needle.

Referring to fig. 10, the magnetic separation magnet assembly 1020 includes a magnetic separation magnet assembly mounting plate 1021, a magnetic separation magnet motor 1022, a magnetic separation magnet fixing member 1023 and a magnetic separation magnet 1024, which form a one-dimensional rotation mechanism with a vertical plane. The magnetic separation magnet motor 1022 is mounted on the magnetic separation magnet assembly mounting plate 1021; the magnetic separation magnet fixing member 1023 is installed on the magnetic separation magnet motor 1022; the magnetic separating magnet 1024 is mounted on the magnetic separating magnet fixing member 1023. Drive magnetic separation magnet mounting 1023 through magnetic separation magnet motor 1022 and rotate to move up during magnetic separation and realize magnetic separation magnet 1024 and test tube contact magnetic separation, drive magnetic separation magnet mounting 1023 through magnetic separation magnet motor 1022 and rotate to move down when magnetic separation is accomplished and realize the action that magnetic separation magnet 1024 and test tube kept away from. During magnetic separation, the magnetic separation magnet motor 1022 drives the magnetic separation magnet 1024 to move upwards to contact the magnetic separation sample tube, and after the magnetic separation is carried out for 15-200s, the magnetic separation needle motor 1014 drives the magnetic separation needle 1013 to move downwards to enter the magnetic separation sample tube to suck out supernatant. Then magnetic separation needle motor 1014 drives magnetic separation needle 1013 to move up and out of sample tube, and magnetic separation magnet motor 1022 drives magnetic separation magnet fixing member 1023 to rotate and move down to realize the action of separating magnetic separation magnet 1024 from the test tube. Thereby completing a magnetic separation cleaning action, and then transferring the sample tube to the sample mixing assembly 600 for dilution liquid.

Referring to fig. 2, after the magnetic separation, the sample reaction container 208 containing the mixture of the sample to be measured and the cytokine reagent B is moved to the cytokine reagent needle loading position 206, and the cytokine reagent a is loaded through the cytokine reagent needle 530 and then mixed uniformly as described above. After the mixing, the sample reaction container 208 containing the sample to be tested, the cytokine reagent a and the reagent B is returned to the flow sample reaction container rack 250 for incubation.

After incubation, the sample reaction container 208 is transferred to a magnetic separation magnet assembly 1020 for magnetic separation to perform microspherical magnetic adsorption, and after the microspherical magnetic adsorption, supernatant is adsorbed in a magnetic separation needle assembly 1010.

The sample reaction vessel 208 after the magnetic separation is run to the reaction vessel and mixed, the diluent level 207 is added, and the diluent is added and mixed through the diluent needle cleaning device 650.

After mixing, the sample reaction container 208 is transferred to a magnetic separation magnet assembly 1020 for microsphere magnetic adsorption, and after microsphere magnetic adsorption, supernatant is adsorbed in a magnetic separation needle assembly 1010.

And (3) operating the sample reaction container 208 after the magnetic separation to the reaction container, uniformly mixing, adding a diluent at a diluent level 207, and adding the diluent through 650 and uniformly mixing. After all the sample reaction vessels 208 are completely filled with the diluent and mixed, the sample reaction vessel rack 250 is retracted to the sample reaction vessel rack pick-and-place position 202 along the sample reaction vessel rack horizontal movement track 201.

The utility model discloses a cytokine's sample preparation facilities can acquire the sample that awaits measuring from the sample container that awaits measuring automatically, will await measuring the sample and add the sample reaction vessel that awaits measuring automatically to automatically, add required multiple cytokine reagent, automatically carry out the mixing to the mixture of sample and various antibody reagents that awaits measuring, guarantee at the reaction of sample and reagent and the quality of hatching the process, avoid any artificial intervention, eliminate during operating personnel exposes in the biological risk of contacting the easy infection of high risk.

The utility model discloses reagent magnetic bead adopts the reciprocal mixing mode of straight line, and occupation space is little, and mixing effect homogeneous. The utility model discloses reagent targets in place and adopts motor and encoder cooperation, and the precision that targets in place is high, good reproducibility. The utility model discloses test tube magnetic separation and needle inhale waste liquid action mutually noninterfere, have fast, the reliability is high, can realize the advantage of test tube intraductal magnetic separation.

The cytokine reagent storage container 303 is provided with one or more vertically arranged reagent containers, and the cytokine reagent a and the reagent B are contained in the reagent containers.

The utility model discloses still including the washing pond that is used for wasing cytokine reagent transmission needle, it is located cytokine reagent transmission assembly 500 next door to wash the pond.

The diluent transmission assembly is provided with a diluent sample adding needle, a diluent sample adding moving mechanism and a sample adding needle cleaning device, and further, the diluent sample adding needle is arranged on the diluent sample adding moving mechanism.

The utility model discloses what key to solve is providing a full-automatic cytokine sample preparation facilities that awaits measuring for flow analysis, obtain the sample that awaits measuring from the sample container that awaits measuring automatically, the sample that awaits measuring is added to the sample reaction vessel that awaits measuring automatically, and add required multiple reagent automatically, mix the mixture of the sample that awaits measuring and various antibody reagent automatically, guarantee at the reaction of sample and reagent and the quality of hatching process, avoid any artificial intervention completely, eliminate operating personnel and expose among the biological risk of contacting the easy infection of high risk. Adopt the utility model discloses replace manual, the application of sample precision is high, the uniformity is good, and the user only need add the sample that awaits measuring, the sample container that awaits measuring, reagent A and B, adopts the utility model discloses a cytokine sample preparation device can accomplish the whole sample preparation of operation according to the flow and the method of setting for are automatic.

Compared with the prior art, the utility model discloses a cytokine's sample preparation facilities can realize carrying out the mixing to the sample that awaits measuring automatically, acquire the sample that awaits measuring from the sample container that awaits measuring automatically, the sample that awaits measuring is added to the sample reaction vessel that awaits measuring automatically, and add required multiple STREAMING reagent automatically, mix to the mixture of sample that awaits measuring and various antibody reagents automatically, guarantee at the quality of the reaction process of sample and reagent, avoid any artificial intervention completely, eliminate operating personnel and expose in the biological risk of the easy infection of contact high risk. An operator only needs to put a sample container to be detected into the sample container rack to be detected, put the sample container rack on the sample autosampler assembly to be detected, obtain a prepared sample to be detected according to a set flow, and then perform flow analysis and detection.

The above description is only for the preferred embodiment of the present invention, and not intended to limit the scope of the present invention, and all modifications made within the spirit of the present invention, equivalent replacements and improvements should be included in the scope of the present invention.

Claims (10)

1. A sample preparation device of cell factors is characterized by comprising an automatic sample injector component of a sample to be detected, a transmission component of the sample to be detected, a sample reaction container component, a cell factor reagent transmission component, a diluent transmission component, a sample blending component, a magnetic separation needle component and a magnetic separation magnet component;

the sample transmission assembly is positioned above the sample autosampler assembly to be detected and the sample reaction container assembly;

the cytokine reagent assembly is positioned at one side of the sample reaction container assembly, and the cytokine reagent transmission assembly is positioned behind the cytokine reagent assembly and the sample reaction container assembly;

the diluent assembly is positioned on one side of the sample reaction container assembly, the diluent transmission assembly is positioned on one side of the diluent assembly, and the sample blending assembly is positioned on one side of the sample reaction container assembly;

the magnetic separation needle assembly is arranged above the sample reaction container assembly, and the magnetic separation magnet assembly is arranged below the sample reaction container assembly and used for magnetic separation.

2. The cytokine sample preparation device according to claim 1, wherein the magnetic separation needle assembly comprises a magnetic separation needle and a needle driving assembly, and the magnetic separation needle is moved up and down by the needle driving assembly; the magnetic separation magnet assembly comprises a magnetic separation magnet and a magnet driving assembly, wherein the magnetic separation magnet rotates under the action of the magnet driving assembly, so that one corner of the magnetic separation magnet contacts the bottom of a sample tube in the sample reaction container assembly to adsorb target magnetic microspheres.

3. The apparatus for preparing a sample of cytokines according to claim 1, wherein the auto-sampler for samples to be tested has a barcode scanning and presence/absence of an identification site of a sample container to be tested, a sample aspirating site for samples to be tested, and a Y-direction in-place sensor, the Y-direction in-place sensor is disposed at an inner edge of the auto-sampler for samples to be tested, the barcode scanning and presence/absence of an identification site of a sample container to be tested is disposed at a left side of the Y-direction in-place sensor, and the sample aspirating site for samples to be tested is disposed at a left side of the barcode scanning and presence/absence of an identification site of a sample container to be tested.

4. The sample preparation device for cytokines according to claim 1, wherein the diluent module comprises a diluent container a and a diluent rotary gate, the diluent container a is suspended from the diluent rotary gate, and the diluent rotary gate is disposed at one side of the sample reaction container module.

5. The sample preparation device for cytokines of claim 1 wherein the diluent transfer assembly includes a transfer pump a and a diluent transfer needle, the transfer pump a and the diluent transfer needle being located behind the sample reaction vessel assembly.

6. The sample preparation device for cytokines according to claim 1, wherein the sample reaction vessel assembly includes a sample reaction vessel holder mechanism and a two-dimensional movement mechanism, the sample reaction vessel holder mechanism being provided on the two-dimensional movement mechanism.

7. The sample preparation device for cytokines according to claim 6, wherein the two-dimensional movement mechanism comprises a rotary driving mechanism and a horizontal driving mechanism, the sample reaction vessel holder mechanism is disposed on the rotary driving mechanism, and the rotary driving mechanism is disposed on the horizontal driving mechanism.

8. The apparatus for preparing cytokine samples according to claim 1, wherein the cytokine reagent assembly comprises a cytokine reagent storage container holder, a rack, and a moving mechanism, the cytokine reagent storage container holder is disposed on the moving mechanism, the cytokine reagent storage container holder is provided with horizontally arranged slots, and cytokine reagent kits are disposed in the slots; the cytokine kit comprises at least two storage containers, microspheres are stored in the storage containers on the outer sides of the storage containers, a pair of fins are arranged at the bottoms of the storage containers, the lower ends of the storage containers are connected with gears, and the gears are in clutch with racks.

9. The cytokine sample preparation device of claim 1, wherein the sample mixing assembly comprises a mixing rotary driving mechanism, a mixing jacking vertical driving mechanism, a mixing jack, a diluent delivery needle and a diluent needle cleaning device, the mixing rotary driving mechanism is arranged on the mixing jacking vertical driving mechanism, the mixing jack is arranged on the mixing rotary driving mechanism, and the diluent needle cleaning device is arranged above the mixing jack.

10. The cytokine sample preparation device of claim 9, wherein the diluent needle cleaning device is sleeved on the diluent delivery needle, and the diluent delivery needle is fixed on the flow sample mixing assembly; the diluent transmission needle, the diluent needle cleaning device and the mixing jack are located on the same axis, and the diluent needle cleaning device and the mixing jack are installed on the same guide rail.

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